Ndc80 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndc80
Entrez Gene ID	32316
Full Name	CG9938 gene product from transcript CG9938-RA
Synonyms	CG9938,Dmel\CG9938,Hec1,NDC80,dNdc80,dmNdc80,hec1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 12A8-12A8

mRNA and Protein(s)

mRNA	Protein	Name
NM_132674.3	NP_572902.1	Ndc80

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

kinetochores movement depends on SPC105R, can occur in the absence of NDC80, and is antagonized by plus-end directed forces from the CENP-E motor.
Title: Lateral and End-On Kinetochore Attachments Are Coordinated to Achieve Bi-orientation in Drosophila Oocytes.

The following Ndc80 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndc80 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01519	NM_132674.3 Latest version!	Drosophila melanogaster Ndc80 (Ndc80), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndc80 ( NM_132674.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572902.1 Drosophila melanogaster Ndc80 (Ndc80), mRNA.

Price & Availability↑

CloneID	OFa01519
Clone ID Related Accession (Same CDS sequence)	NM_132674.3
Accession Version	NM_132674.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ndc80
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132674.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCACC TGATGCCCCG GCGGACCAGC GAGTTCCGTG GCGATGTACT GGAGGAGCGA 
GGCACTGCCG ATAAGCGGCA AACCAGGGCA ACAAAGCGGA GCGCAGCCAC GCACACTCCG 
TCCTCCAGCC AGGCGGCACC CAGAAGGCCG TCCGCATCGC GTCTGCCGCA GCCGCTGAAC 
TTGGAGCGCG ATCGGGCCAG TCAAATGGGC GTGACGCCCA AGCGAGGCCA CCGGTTTGTG 
CCCTCCACTG CGGAGCGCGC CATTGCGCCA CACTCCGACA AGAAATGGGT GGCGGAGAGG 
GCACAGCAGA TCCTGGAATA CCTACATGGC ATTCAGAACA GCGAGGCGCC CACTGGACTT 
ATTGCGGATC TATTCAGTCG GCCCGGGGGT TTGCGACACA TGACCATCAA GCAATTTGTT 
TCCATTCTCA ACTTTATGTT CCACCACATC TGGCGAAATC GCGTGACAGT GGGCCAGAAC 
CATGTCGAGG ATATTACCAG CGCAATGCAA AAGCTACAGT ATCCTTATCA GGTGAACAAA 
TCATGGCTTG TATCGCCAAC AACACAGCAT TCATTCGGCC ATGTGATCGT TCTGCTGGAC 
TTTCTCATGG ACTTTTTACC GCCTCTGCCC TCGTCGGATG TGGTGGAGGA GGAGTTTCCG 
TTTATGGAAA CCATGGAGCA GCCCAGCTCG TATCTGAATA GCATGCACTG CGAGTCGACG 
ACCATCATGT CCACCACACA GGCGCACGCC ATTCAGCTAG ATGAAGACGT CAATGGTCTG 
CTCTTCGAAG AGGCTAGCAA ATGCATTGCC TTGTGGGATC AGGAGCTTAC CAATGAGGAG 
GCCAAGCTGC AGGCGGAGAC CAGGGATCAG GTGATCAGCA AGAAGTGTGA TTTGCCCGAT 
CGCAAAGCCT TAGACCAGCT AATCGGCGAT CTGAAGACGA AACTACAGCA ACTGGAGAAT 
AAGTTGCATG ATCCCAGTGA AGACAATAAA CTGAACAAAC TGGAACGTCT TACCAAAGAG 
CACGATCAGC TTGCCCAACA GCTAGCTGCC AGTGAAGAGG AGCTGATCAA GAAACTGAAG 
TTATACGAAC AGCTGTCTGC CCAGGTAGAA GAAAAACAGT CTAATCTCCG CCAGCAAATG 
AAGTATGAAA AGCGTCTGGA GCAGGCGGTT AACAGGCAGA AGTTCTCCGC CCAGCAGCTA 
AAGGAGCTTC AAATGAAGTG CGACGATATG GAAAACTACT CCAAGGCTTA TGAGCGCCAG 
GTGAAGGAGG TGTCCGAGCT GGAGCTGCAT CAGCAGGTGA TGCTGTCGCG CGCCAAGCAA 
AAGCAACTGG ATTCCGTTGA GGTCTTTAAC TCGCACGTGC GCCACCTGAG CATGGACCCG 
GTGATTTGTG GCCTGATAAA GAGCGGCATT GGCCAGCAAT CGGACCTTAC TCTGCCACTA 
AATCCCAATC AAGAGGACAT CTCGGAGAGG GTGCAGTGCC TGGAGTTGCT GGGCAAACTT 
CTGCAACAGC ACCGCCAGCA GAATATCGAT CGGCGTCAGA TGTTGGACAA GCAGGTGGCC 
AAGGTAAAGT CTGATTCCAT CGAGTTAGAC ACCGAAATCG CCACCATGGA CTCTCAGTTG 
CGGGCGCAGA AACAGCGTCT GACCAAAATG GAGGCCAGTT ATCGGACCAA GCGCGACATG 
CAGGCGCAGC ACAGGCAACA GCTGCTCGAG GATCAGTACG ACCAGACCGC CCGTCTGGAT 
GAGTTGGAGA AGAGGGAGAA GCAGGCGCTG GAAAAGCTGG AAGCCAGCAA GCAACGGAAC 
GAGGATCTAC TCACCGCTGC TGAGCAGTTT CAGGAGCAGG ATCTCAAGGC CCGCCAAGAT 
CGCCTGGAAA AATGCGAGCA GAAGCTGGCC AAAGCGGAGG ACCAGCTGCT CGCCGTGGAA 
TCAAAGGTCA ACGCGACTCA GGCCAAGCTG AACGAGGTGG AGCAAAAGGT GTACTCCGCC 
AAGCTGCCAT CCTTTCAGCC CGTGCTAGAT GCCATCAAGA ATCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572902.1
CDS	98..2125
Translation	MSHLMPRRTSEFRGDVLEERGTADKRQTRATKRSAATHTPSSSQAAPRRPSASRLPQPLNLERDRASQMGVTPKRGHRFVPSTAERAIAPHSDKKWVAERAQQILEYLHGIQNSEAPTGLIADLFSRPGGLRHMTIKQFVSILNFMFHHIWRNRVTVGQNHVEDITSAMQKLQYPYQVNKSWLVSPTTQHSFGHVIVLLDFLMDFLPPLPSSDVVEEEFPFMETMEQPSSYLNSMHCESTTIMSTTQAHAIQLDEDVNGLLFEEASKCIALWDQELTNEEAKLQAETRDQVISKKCDLPDRKALDQLIGDLKTKLQQLENKLHDPSEDNKLNKLERLTKEHDQLAQQLAASEEELIKKLKLYEQLSAQVEEKQSNLRQQMKYEKRLEQAVNRQKFSAQQLKELQMKCDDMENYSKAYERQVKEVSELELHQQVMLSRAKQKQLDSVEVFNSHVRHLSMDPVICGLIKSGIGQQSDLTLPLNPNQEDISERVQCLELLGKLLQQHRQQNIDRRQMLDKQVAKVKSDSIELDTEIATMDSQLRAQKQRLTKMEASYRTKRDMQAQHRQQLLEDQYDQTARLDELEKREKQALEKLEASKQRNEDLLTAAEQFQEQDLKARQDRLEKCEQKLAKAEDQLLAVESKVNATQAKLNEVEQKVYSAKLPSFQPVLDAIKNH

Target ORF information:

RefSeq Version	NM_132674.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ndc80 (Ndc80), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132674.3

ORF Insert Sequence:

ATGTCGCACC TGATGCCCCG GCGGACCAGC GAGTTCCGTG GCGATGTACT GGAGGAGCGA 
GGCACTGCCG ATAAGCGGCA AACCAGGGCA ACAAAGCGGA GCGCAGCCAC GCACACTCCG 
TCCTCCAGCC AGGCGGCACC CAGAAGGCCG TCCGCATCGC GTCTGCCGCA GCCGCTGAAC 
TTGGAGCGCG ATCGGGCCAG TCAAATGGGC GTGACGCCCA AGCGAGGCCA CCGGTTTGTG 
CCCTCCACTG CGGAGCGCGC CATTGCGCCA CACTCCGACA AGAAATGGGT GGCGGAGAGG 
GCACAGCAGA TCCTGGAATA CCTACATGGC ATTCAGAACA GCGAGGCGCC CACTGGACTT 
ATTGCGGATC TATTCAGTCG GCCCGGGGGT TTGCGACACA TGACCATCAA GCAATTTGTT 
TCCATTCTCA ACTTTATGTT CCACCACATC TGGCGAAATC GCGTGACAGT GGGCCAGAAC 
CATGTCGAGG ATATTACCAG CGCAATGCAA AAGCTACAGT ATCCTTATCA GGTGAACAAA 
TCATGGCTTG TATCGCCAAC AACACAGCAT TCATTCGGCC ATGTGATCGT TCTGCTGGAC 
TTTCTCATGG ACTTTTTACC GCCTCTGCCC TCGTCGGATG TGGTGGAGGA GGAGTTTCCG 
TTTATGGAAA CCATGGAGCA GCCCAGCTCG TATCTGAATA GCATGCACTG CGAGTCGACG 
ACCATCATGT CCACCACACA GGCGCACGCC ATTCAGCTAG ATGAAGACGT CAATGGTCTG 
CTCTTCGAAG AGGCTAGCAA ATGCATTGCC TTGTGGGATC AGGAGCTTAC CAATGAGGAG 
GCCAAGCTGC AGGCGGAGAC CAGGGATCAG GTGATCAGCA AGAAGTGTGA TTTGCCCGAT 
CGCAAAGCCT TAGACCAGCT AATCGGCGAT CTGAAGACGA AACTACAGCA ACTGGAGAAT 
AAGTTGCATG ATCCCAGTGA AGACAATAAA CTGAACAAAC TGGAACGTCT TACCAAAGAG 
CACGATCAGC TTGCCCAACA GCTAGCTGCC AGTGAAGAGG AGCTGATCAA GAAACTGAAG 
TTATACGAAC AGCTGTCTGC CCAGGTAGAA GAAAAACAGT CTAATCTCCG CCAGCAAATG 
AAGTATGAAA AGCGTCTGGA GCAGGCGGTT AACAGGCAGA AGTTCTCCGC CCAGCAGCTA 
AAGGAGCTTC AAATGAAGTG CGACGATATG GAAAACTACT CCAAGGCTTA TGAGCGCCAG 
GTGAAGGAGG TGTCCGAGCT GGAGCTGCAT CAGCAGGTGA TGCTGTCGCG CGCCAAGCAA 
AAGCAACTGG ATTCCGTTGA GGTCTTTAAC TCGCACGTGC GCCACCTGAG CATGGACCCG 
GTGATTTGTG GCCTGATAAA GAGCGGCATT GGCCAGCAAT CGGACCTTAC TCTGCCACTA 
AATCCCAATC AAGAGGACAT CTCGGAGAGG GTGCAGTGCC TGGAGTTGCT GGGCAAACTT 
CTGCAACAGC ACCGCCAGCA GAATATCGAT CGGCGTCAGA TGTTGGACAA GCAGGTGGCC 
AAGGTAAAGT CTGATTCCAT CGAGTTAGAC ACCGAAATCG CCACCATGGA CTCTCAGTTG 
CGGGCGCAGA AACAGCGTCT GACCAAAATG GAGGCCAGTT ATCGGACCAA GCGCGACATG 
CAGGCGCAGC ACAGGCAACA GCTGCTCGAG GATCAGTACG ACCAGACCGC CCGTCTGGAT 
GAGTTGGAGA AGAGGGAGAA GCAGGCGCTG GAAAAGCTGG AAGCCAGCAA GCAACGGAAC 
GAGGATCTAC TCACCGCTGC TGAGCAGTTT CAGGAGCAGG ATCTCAAGGC CCGCCAAGAT 
CGCCTGGAAA AATGCGAGCA GAAGCTGGCC AAAGCGGAGG ACCAGCTGCT CGCCGTGGAA 
TCAAAGGTCA ACGCGACTCA GGCCAAGCTG AACGAGGTGG AGCAAAAGGT GTACTCCGCC 
AAGCTGCCAT CCTTTCAGCC CGTGCTAGAT GCCATCAAGA ATCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ndc80 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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