CG43313 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG43313
Entrez Gene ID	32259
Full Name	CG43313 gene product from transcript CG43313-RB
Synonyms	CG12714,CG4351,Dmel\CG43313,Dmel_CG12714,Dmel_CG4351,dCHPF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11D11-11E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298255.1	NP_001285184.1	uncharacterized protein, isoform B
NM_132625.3	NP_572853.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Chpf	NP_001005906.1
Danio rerio (zebrafish)	chpfa	XP_002663375.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001050	XP_318249.4
Canis lupus familiaris (dog)	CHPF	XP_003640243.1
Drosophila melanogaster (fruit fly)	CG43313	NP_572853.2
Bos taurus (cattle)	CHPF	NP_001192680.1
Mus musculus (house mouse)	Chpf	NP_001001566.1
Danio rerio (zebrafish)	LOC794660	XP_001923418.1
Caenorhabditis elegans (roundworm)	mig-22	NP_498934.1
Homo sapiens (human)	CHPF	NP_078812.2
Pan troglodytes (chimpanzee)	CHPF	XP_003309517.1
	CHPF	XP_001104808.1
Gallus gallus (chicken)	CHPF	XP_001232317.2
Xenopus tropicalis (tropical clawed frog)	chpf	XP_002942593.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG43313 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG43313 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01456	NM_132625.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG43313), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01456	NM_001298255.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG43313), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG43313 ( NM_001298255.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285184.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG43313), mRNA.

Price & Availability↑

CloneID	OFa01456
Clone ID Related Accession (Same CDS sequence)	NM_001298255.1 , NM_132625.3
Accession Version	NM_001298255.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGAGGC CCTTTACAGC TTTGAATCGC AATCACTACT TCGTCATCGG CCTGCTCCTG 
GGCCTCCTGC TCAGCTGGCA CATTCCGGAG GACATCTGGG AGGAGGAGTG TCCCGAGGAG 
GCCGCCGAGA ATCTGCTAAT CGAGCGATTT GGCCAGGAGT TCGAGCCCCA CTTGAACCTG 
ATCAACAAGC CGCTGGCGGC CAAGAAGCCG GTGAAAAATG TGATTCGACC GCGATACTAC 
TCCTCAGAGT TGGGTATACG GGAGAAGCTC TTCATCGGGG TGATGACCTC GCAGGAGCAC 
ATCAACACCT TTGCGACGGC CTTCAACCGC ACCACCGCCC ACCTGGTGAA CAAGATCAAG 
TTCTTCATTT ATGCGGACAG TGTAAAGACC AACTACAAGC TGAAGAACAT CGTGGGATTT 
ACGGACACGC GGGAGAGTCG CCGGCCATTC CACGTGGTCA AGTATATTGC GGACAACTAT 
CTGGATGAGT ACGATTATTT CCTGTTGGTG CCCGATACCG TCTACGTGGA TGCGCGTAAG 
CTGGTGAAGT TGCTCTATCA CATGAGCATC ACCTTTGACC TGTACATGGG TGGCGCACGC 
ATCGGCCTGG ATCCTTCGGG TGGCGGTGCC TCCGCCGATG GCCAGTCGAA TGAACCGCCG 
GCGAACGAGG AGGAGGCACC CGGAGCCAGT GATCGCAACT ATTGCTCCTT GGAGGCGGGC 
ATTCTGCTCA GCAGCAGCGT CATACGCAAG ATGCGCAACA ATCTGGAGCG CTGCGTCCGG 
ATCGGAAGCA CCAGTGACCA TAGTGTCAAC ATCGGGCGGT GCGTCAAGTA CGCCAGTCGG 
GTGGCCGGAT GCCAGGAGAG CTTTCAGGGC ATGCGGCAAT TTAGCTACGC CTTGGATGCG 
CCGGGTCGTC GTCATCGCGA ATTCAGCGAA TTGGCCAAAG AGGAGGCCTT CCGCAATGCC 
AGCACCGTGT ATCCCGTCCA GACGCCGGAG GACTTCTACC GATTGCATGC CTACTACTCC 
AAGCACCATC TGGAGAAGGT GCAGGAGCGG GGCTATGCTC TAGAGCAGAA ATCGTACCGG 
ATTGCCAATG GGAGTATATC GAACAAGATT CTCGAGATCC GGTGGCCATT GGGCGTGCCG 
CCACCTAGTG CGCCGGAGAC GCGTCACGAC ATCCTCACGT GGCAGCTGCT CAATGGGACG 
CACAACTTTC TGCCCAATGG CAATGCGGAG CATGCGGTGG CAACGCTGTC GCGGATCGAG 
GCACAGGACT TTGCCAAAGT CCTGGAGATA GCACTACAGT ATGCCGCACT TAAGCATCCG 
CGCCTGAGCT ACCACAGTCT GCACAGTGCC TATCGGAAGT TCGATGCCAC ACGCGGCATG 
GACTACCAGC TGCATCTCAA TCTGCAGGAG GGATCGGGTC GCAGTCGCCG GCTCGTGATC 
AAGAGCTTCG AGGTGGTTAA GCCACTGGGT CGCGTGGAGG TGGTGCCCTC GCCCTACGTC 
ACCGAGAGCA CAAGAATTGC CATGTTGGTG CCCGCCTTTG AGCACCAGGT GCCGGATGCT 
CTGCTGTTTG TGGAACAGTA CGAAAGGATT TGTATGCAAA ATCAGGACAA CACATTCCTG 
TTGCTGATCT TCATGTACCG ACTGGAGTCG CCCAGCAAGG GCGACGAGGA TCCCTTCAAA 
GCACTGAAGA CACTGGCCCT TGACTTGTCA TCTAAGTACA AGACCGATGG CTCTCGGATC 
GCCTGGGTGT CCATCCGGCT GCCGGAGCAG CTGAGCGAAC CGGTGGATCC GCAGAGCTGG 
CTGCTGCACG CCTCCATGTA TGGACCACGC CAGCTTCTCA GTCTGGTGGT TGCGGATCTG 
GCACTGCCCA AACTGGGCCT CGAATCCCTC GTGCTGCTGG CCACGCCAGG CATGGTCTTC 
AAGGCGGACT TTCTGAATCG CGTGCGCATG AACACGATAC AGGGCTTCCA GGTGTACGCA 
CCAATCGGCT TCCAGATGTA CCCATGCCGC TGGGCGCATT TCTGCCGTGA GTGCGACACC 
TGCGACGTGA GCCAGAGCAG CGGTTACTTC GATCGCCATA ACCACGACGT GATCGCTTTC 
TATAGTCGGG ATTATGTCCA GGCTCGCAAG CTCTTACATC CGCAGGGACT GCCGATCATA 
CGCAGCGACC TGGACATCGA CCAGCTGCTG CTGCAGCCTG GCGAGGAGAC GCGACCACCG 
GGCGTGGAAA GCATTCTCGA TATGTTCGTG GCGGCACAGC ACTCGGTGCA TATACTGCGC 
GGAGTGGAAC CGAATTTGCG CTTCGGCCAG GATGTGCGGA ATCACTTGGC ACGCGGAGGA 
ACGCTGCCGC AGAGCGTGCC GGAACGCTGC GGTCGGGAGC AGTGCATCCA CCTGGCGTCG 
CGCAAGCAGA TCGGCGACGC CATAATACGC TACGAGGACA AAAGTATTCT ACACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285184.1
CDS	620..3079
Translation	MRRPFTALNRNHYFVIGLLLGLLLSWHIPEDIWEEECPEEAAENLLIERFGQEFEPHLNLINKPLAAKKPVKNVIRPRYYSSELGIREKLFIGVMTSQEHINTFATAFNRTTAHLVNKIKFFIYADSVKTNYKLKNIVGFTDTRESRRPFHVVKYIADNYLDEYDYFLLVPDTVYVDARKLVKLLYHMSITFDLYMGGARIGLDPSGGGASADGQSNEPPANEEEAPGASDRNYCSLEAGILLSSSVIRKMRNNLERCVRIGSTSDHSVNIGRCVKYASRVAGCQESFQGMRQFSYALDAPGRRHREFSELAKEEAFRNASTVYPVQTPEDFYRLHAYYSKHHLEKVQERGYALEQKSYRIANGSISNKILEIRWPLGVPPPSAPETRHDILTWQLLNGTHNFLPNGNAEHAVATLSRIEAQDFAKVLEIALQYAALKHPRLSYHSLHSAYRKFDATRGMDYQLHLNLQEGSGRSRRLVIKSFEVVKPLGRVEVVPSPYVTESTRIAMLVPAFEHQVPDALLFVEQYERICMQNQDNTFLLLIFMYRLESPSKGDEDPFKALKTLALDLSSKYKTDGSRIAWVSIRLPEQLSEPVDPQSWLLHASMYGPRQLLSLVVADLALPKLGLESLVLLATPGMVFKADFLNRVRMNTIQGFQVYAPIGFQMYPCRWAHFCRECDTCDVSQSSGYFDRHNHDVIAFYSRDYVQARKLLHPQGLPIIRSDLDIDQLLLQPGEETRPPGVESILDMFVAAQHSVHILRGVEPNLRFGQDVRNHLARGGTLPQSVPERCGREQCIHLASRKQIGDAIIRYEDKSILHK

Target ORF information:

RefSeq Version	NM_001298255.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG43313), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298255.1

ORF Insert Sequence:

ATGCGGAGGC CCTTTACAGC TTTGAATCGC AATCACTACT TCGTCATCGG CCTGCTCCTG 
GGCCTCCTGC TCAGCTGGCA CATTCCGGAG GACATCTGGG AGGAGGAGTG TCCCGAGGAG 
GCCGCCGAGA ATCTGCTAAT CGAGCGATTT GGCCAGGAGT TCGAGCCCCA CTTGAACCTG 
ATCAACAAGC CGCTGGCGGC CAAGAAGCCG GTGAAAAATG TGATTCGACC GCGATACTAC 
TCCTCAGAGT TGGGTATACG GGAGAAGCTC TTCATCGGGG TGATGACCTC GCAGGAGCAC 
ATCAACACCT TTGCGACGGC CTTCAACCGC ACCACCGCCC ACCTGGTGAA CAAGATCAAG 
TTCTTCATTT ATGCGGACAG TGTAAAGACC AACTACAAGC TGAAGAACAT CGTGGGATTT 
ACGGACACGC GGGAGAGTCG CCGGCCATTC CACGTGGTCA AGTATATTGC GGACAACTAT 
CTGGATGAGT ACGATTATTT CCTGTTGGTG CCCGATACCG TCTACGTGGA TGCGCGTAAG 
CTGGTGAAGT TGCTCTATCA CATGAGCATC ACCTTTGACC TGTACATGGG TGGCGCACGC 
ATCGGCCTGG ATCCTTCGGG TGGCGGTGCC TCCGCCGATG GCCAGTCGAA TGAACCGCCG 
GCGAACGAGG AGGAGGCACC CGGAGCCAGT GATCGCAACT ATTGCTCCTT GGAGGCGGGC 
ATTCTGCTCA GCAGCAGCGT CATACGCAAG ATGCGCAACA ATCTGGAGCG CTGCGTCCGG 
ATCGGAAGCA CCAGTGACCA TAGTGTCAAC ATCGGGCGGT GCGTCAAGTA CGCCAGTCGG 
GTGGCCGGAT GCCAGGAGAG CTTTCAGGGC ATGCGGCAAT TTAGCTACGC CTTGGATGCG 
CCGGGTCGTC GTCATCGCGA ATTCAGCGAA TTGGCCAAAG AGGAGGCCTT CCGCAATGCC 
AGCACCGTGT ATCCCGTCCA GACGCCGGAG GACTTCTACC GATTGCATGC CTACTACTCC 
AAGCACCATC TGGAGAAGGT GCAGGAGCGG GGCTATGCTC TAGAGCAGAA ATCGTACCGG 
ATTGCCAATG GGAGTATATC GAACAAGATT CTCGAGATCC GGTGGCCATT GGGCGTGCCG 
CCACCTAGTG CGCCGGAGAC GCGTCACGAC ATCCTCACGT GGCAGCTGCT CAATGGGACG 
CACAACTTTC TGCCCAATGG CAATGCGGAG CATGCGGTGG CAACGCTGTC GCGGATCGAG 
GCACAGGACT TTGCCAAAGT CCTGGAGATA GCACTACAGT ATGCCGCACT TAAGCATCCG 
CGCCTGAGCT ACCACAGTCT GCACAGTGCC TATCGGAAGT TCGATGCCAC ACGCGGCATG 
GACTACCAGC TGCATCTCAA TCTGCAGGAG GGATCGGGTC GCAGTCGCCG GCTCGTGATC 
AAGAGCTTCG AGGTGGTTAA GCCACTGGGT CGCGTGGAGG TGGTGCCCTC GCCCTACGTC 
ACCGAGAGCA CAAGAATTGC CATGTTGGTG CCCGCCTTTG AGCACCAGGT GCCGGATGCT 
CTGCTGTTTG TGGAACAGTA CGAAAGGATT TGTATGCAAA ATCAGGACAA CACATTCCTG 
TTGCTGATCT TCATGTACCG ACTGGAGTCG CCCAGCAAGG GCGACGAGGA TCCCTTCAAA 
GCACTGAAGA CACTGGCCCT TGACTTGTCA TCTAAGTACA AGACCGATGG CTCTCGGATC 
GCCTGGGTGT CCATCCGGCT GCCGGAGCAG CTGAGCGAAC CGGTGGATCC GCAGAGCTGG 
CTGCTGCACG CCTCCATGTA TGGACCACGC CAGCTTCTCA GTCTGGTGGT TGCGGATCTG 
GCACTGCCCA AACTGGGCCT CGAATCCCTC GTGCTGCTGG CCACGCCAGG CATGGTCTTC 
AAGGCGGACT TTCTGAATCG CGTGCGCATG AACACGATAC AGGGCTTCCA GGTGTACGCA 
CCAATCGGCT TCCAGATGTA CCCATGCCGC TGGGCGCATT TCTGCCGTGA GTGCGACACC 
TGCGACGTGA GCCAGAGCAG CGGTTACTTC GATCGCCATA ACCACGACGT GATCGCTTTC 
TATAGTCGGG ATTATGTCCA GGCTCGCAAG CTCTTACATC CGCAGGGACT GCCGATCATA 
CGCAGCGACC TGGACATCGA CCAGCTGCTG CTGCAGCCTG GCGAGGAGAC GCGACCACCG 
GGCGTGGAAA GCATTCTCGA TATGTTCGTG GCGGCACAGC ACTCGGTGCA TATACTGCGC 
GGAGTGGAAC CGAATTTGCG CTTCGGCCAG GATGTGCGGA ATCACTTGGC ACGCGGAGGA 
ACGCTGCCGC AGAGCGTGCC GGAACGCTGC GGTCGGGAGC AGTGCATCCA CCTGGCGTCG 
CGCAAGCAGA TCGGCGACGC CATAATACGC TACGAGGACA AAAGTATTCT ACACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG43313 ( NM_132625.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572853.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG43313), mRNA.

Price & Availability↑

CloneID	OFa01456
Clone ID Related Accession (Same CDS sequence)	NM_001298255.1 , NM_132625.3
Accession Version	NM_132625.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2460bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132625.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGAGGC CCTTTACAGC TTTGAATCGC AATCACTACT TCGTCATCGG CCTGCTCCTG 
GGCCTCCTGC TCAGCTGGCA CATTCCGGAG GACATCTGGG AGGAGGAGTG TCCCGAGGAG 
GCCGCCGAGA ATCTGCTAAT CGAGCGATTT GGCCAGGAGT TCGAGCCCCA CTTGAACCTG 
ATCAACAAGC CGCTGGCGGC CAAGAAGCCG GTGAAAAATG TGATTCGACC GCGATACTAC 
TCCTCAGAGT TGGGTATACG GGAGAAGCTC TTCATCGGGG TGATGACCTC GCAGGAGCAC 
ATCAACACCT TTGCGACGGC CTTCAACCGC ACCACCGCCC ACCTGGTGAA CAAGATCAAG 
TTCTTCATTT ATGCGGACAG TGTAAAGACC AACTACAAGC TGAAGAACAT CGTGGGATTT 
ACGGACACGC GGGAGAGTCG CCGGCCATTC CACGTGGTCA AGTATATTGC GGACAACTAT 
CTGGATGAGT ACGATTATTT CCTGTTGGTG CCCGATACCG TCTACGTGGA TGCGCGTAAG 
CTGGTGAAGT TGCTCTATCA CATGAGCATC ACCTTTGACC TGTACATGGG TGGCGCACGC 
ATCGGCCTGG ATCCTTCGGG TGGCGGTGCC TCCGCCGATG GCCAGTCGAA TGAACCGCCG 
GCGAACGAGG AGGAGGCACC CGGAGCCAGT GATCGCAACT ATTGCTCCTT GGAGGCGGGC 
ATTCTGCTCA GCAGCAGCGT CATACGCAAG ATGCGCAACA ATCTGGAGCG CTGCGTCCGG 
ATCGGAAGCA CCAGTGACCA TAGTGTCAAC ATCGGGCGGT GCGTCAAGTA CGCCAGTCGG 
GTGGCCGGAT GCCAGGAGAG CTTTCAGGGC ATGCGGCAAT TTAGCTACGC CTTGGATGCG 
CCGGGTCGTC GTCATCGCGA ATTCAGCGAA TTGGCCAAAG AGGAGGCCTT CCGCAATGCC 
AGCACCGTGT ATCCCGTCCA GACGCCGGAG GACTTCTACC GATTGCATGC CTACTACTCC 
AAGCACCATC TGGAGAAGGT GCAGGAGCGG GGCTATGCTC TAGAGCAGAA ATCGTACCGG 
ATTGCCAATG GGAGTATATC GAACAAGATT CTCGAGATCC GGTGGCCATT GGGCGTGCCG 
CCACCTAGTG CGCCGGAGAC GCGTCACGAC ATCCTCACGT GGCAGCTGCT CAATGGGACG 
CACAACTTTC TGCCCAATGG CAATGCGGAG CATGCGGTGG CAACGCTGTC GCGGATCGAG 
GCACAGGACT TTGCCAAAGT CCTGGAGATA GCACTACAGT ATGCCGCACT TAAGCATCCG 
CGCCTGAGCT ACCACAGTCT GCACAGTGCC TATCGGAAGT TCGATGCCAC ACGCGGCATG 
GACTACCAGC TGCATCTCAA TCTGCAGGAG GGATCGGGTC GCAGTCGCCG GCTCGTGATC 
AAGAGCTTCG AGGTGGTTAA GCCACTGGGT CGCGTGGAGG TGGTGCCCTC GCCCTACGTC 
ACCGAGAGCA CAAGAATTGC CATGTTGGTG CCCGCCTTTG AGCACCAGGT GCCGGATGCT 
CTGCTGTTTG TGGAACAGTA CGAAAGGATT TGTATGCAAA ATCAGGACAA CACATTCCTG 
TTGCTGATCT TCATGTACCG ACTGGAGTCG CCCAGCAAGG GCGACGAGGA TCCCTTCAAA 
GCACTGAAGA CACTGGCCCT TGACTTGTCA TCTAAGTACA AGACCGATGG CTCTCGGATC 
GCCTGGGTGT CCATCCGGCT GCCGGAGCAG CTGAGCGAAC CGGTGGATCC GCAGAGCTGG 
CTGCTGCACG CCTCCATGTA TGGACCACGC CAGCTTCTCA GTCTGGTGGT TGCGGATCTG 
GCACTGCCCA AACTGGGCCT CGAATCCCTC GTGCTGCTGG CCACGCCAGG CATGGTCTTC 
AAGGCGGACT TTCTGAATCG CGTGCGCATG AACACGATAC AGGGCTTCCA GGTGTACGCA 
CCAATCGGCT TCCAGATGTA CCCATGCCGC TGGGCGCATT TCTGCCGTGA GTGCGACACC 
TGCGACGTGA GCCAGAGCAG CGGTTACTTC GATCGCCATA ACCACGACGT GATCGCTTTC 
TATAGTCGGG ATTATGTCCA GGCTCGCAAG CTCTTACATC CGCAGGGACT GCCGATCATA 
CGCAGCGACC TGGACATCGA CCAGCTGCTG CTGCAGCCTG GCGAGGAGAC GCGACCACCG 
GGCGTGGAAA GCATTCTCGA TATGTTCGTG GCGGCACAGC ACTCGGTGCA TATACTGCGC 
GGAGTGGAAC CGAATTTGCG CTTCGGCCAG GATGTGCGGA ATCACTTGGC ACGCGGAGGA 
ACGCTGCCGC AGAGCGTGCC GGAACGCTGC GGTCGGGAGC AGTGCATCCA CCTGGCGTCG 
CGCAAGCAGA TCGGCGACGC CATAATACGC TACGAGGACA AAAGTATTCT ACACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572853.2
CDS	620..3079
Translation	MRRPFTALNRNHYFVIGLLLGLLLSWHIPEDIWEEECPEEAAENLLIERFGQEFEPHLNLINKPLAAKKPVKNVIRPRYYSSELGIREKLFIGVMTSQEHINTFATAFNRTTAHLVNKIKFFIYADSVKTNYKLKNIVGFTDTRESRRPFHVVKYIADNYLDEYDYFLLVPDTVYVDARKLVKLLYHMSITFDLYMGGARIGLDPSGGGASADGQSNEPPANEEEAPGASDRNYCSLEAGILLSSSVIRKMRNNLERCVRIGSTSDHSVNIGRCVKYASRVAGCQESFQGMRQFSYALDAPGRRHREFSELAKEEAFRNASTVYPVQTPEDFYRLHAYYSKHHLEKVQERGYALEQKSYRIANGSISNKILEIRWPLGVPPPSAPETRHDILTWQLLNGTHNFLPNGNAEHAVATLSRIEAQDFAKVLEIALQYAALKHPRLSYHSLHSAYRKFDATRGMDYQLHLNLQEGSGRSRRLVIKSFEVVKPLGRVEVVPSPYVTESTRIAMLVPAFEHQVPDALLFVEQYERICMQNQDNTFLLLIFMYRLESPSKGDEDPFKALKTLALDLSSKYKTDGSRIAWVSIRLPEQLSEPVDPQSWLLHASMYGPRQLLSLVVADLALPKLGLESLVLLATPGMVFKADFLNRVRMNTIQGFQVYAPIGFQMYPCRWAHFCRECDTCDVSQSSGYFDRHNHDVIAFYSRDYVQARKLLHPQGLPIIRSDLDIDQLLLQPGEETRPPGVESILDMFVAAQHSVHILRGVEPNLRFGQDVRNHLARGGTLPQSVPERCGREQCIHLASRKQIGDAIIRYEDKSILHK

Target ORF information:

RefSeq Version	NM_132625.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG43313), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132625.3

ORF Insert Sequence:

ATGCGGAGGC CCTTTACAGC TTTGAATCGC AATCACTACT TCGTCATCGG CCTGCTCCTG 
GGCCTCCTGC TCAGCTGGCA CATTCCGGAG GACATCTGGG AGGAGGAGTG TCCCGAGGAG 
GCCGCCGAGA ATCTGCTAAT CGAGCGATTT GGCCAGGAGT TCGAGCCCCA CTTGAACCTG 
ATCAACAAGC CGCTGGCGGC CAAGAAGCCG GTGAAAAATG TGATTCGACC GCGATACTAC 
TCCTCAGAGT TGGGTATACG GGAGAAGCTC TTCATCGGGG TGATGACCTC GCAGGAGCAC 
ATCAACACCT TTGCGACGGC CTTCAACCGC ACCACCGCCC ACCTGGTGAA CAAGATCAAG 
TTCTTCATTT ATGCGGACAG TGTAAAGACC AACTACAAGC TGAAGAACAT CGTGGGATTT 
ACGGACACGC GGGAGAGTCG CCGGCCATTC CACGTGGTCA AGTATATTGC GGACAACTAT 
CTGGATGAGT ACGATTATTT CCTGTTGGTG CCCGATACCG TCTACGTGGA TGCGCGTAAG 
CTGGTGAAGT TGCTCTATCA CATGAGCATC ACCTTTGACC TGTACATGGG TGGCGCACGC 
ATCGGCCTGG ATCCTTCGGG TGGCGGTGCC TCCGCCGATG GCCAGTCGAA TGAACCGCCG 
GCGAACGAGG AGGAGGCACC CGGAGCCAGT GATCGCAACT ATTGCTCCTT GGAGGCGGGC 
ATTCTGCTCA GCAGCAGCGT CATACGCAAG ATGCGCAACA ATCTGGAGCG CTGCGTCCGG 
ATCGGAAGCA CCAGTGACCA TAGTGTCAAC ATCGGGCGGT GCGTCAAGTA CGCCAGTCGG 
GTGGCCGGAT GCCAGGAGAG CTTTCAGGGC ATGCGGCAAT TTAGCTACGC CTTGGATGCG 
CCGGGTCGTC GTCATCGCGA ATTCAGCGAA TTGGCCAAAG AGGAGGCCTT CCGCAATGCC 
AGCACCGTGT ATCCCGTCCA GACGCCGGAG GACTTCTACC GATTGCATGC CTACTACTCC 
AAGCACCATC TGGAGAAGGT GCAGGAGCGG GGCTATGCTC TAGAGCAGAA ATCGTACCGG 
ATTGCCAATG GGAGTATATC GAACAAGATT CTCGAGATCC GGTGGCCATT GGGCGTGCCG 
CCACCTAGTG CGCCGGAGAC GCGTCACGAC ATCCTCACGT GGCAGCTGCT CAATGGGACG 
CACAACTTTC TGCCCAATGG CAATGCGGAG CATGCGGTGG CAACGCTGTC GCGGATCGAG 
GCACAGGACT TTGCCAAAGT CCTGGAGATA GCACTACAGT ATGCCGCACT TAAGCATCCG 
CGCCTGAGCT ACCACAGTCT GCACAGTGCC TATCGGAAGT TCGATGCCAC ACGCGGCATG 
GACTACCAGC TGCATCTCAA TCTGCAGGAG GGATCGGGTC GCAGTCGCCG GCTCGTGATC 
AAGAGCTTCG AGGTGGTTAA GCCACTGGGT CGCGTGGAGG TGGTGCCCTC GCCCTACGTC 
ACCGAGAGCA CAAGAATTGC CATGTTGGTG CCCGCCTTTG AGCACCAGGT GCCGGATGCT 
CTGCTGTTTG TGGAACAGTA CGAAAGGATT TGTATGCAAA ATCAGGACAA CACATTCCTG 
TTGCTGATCT TCATGTACCG ACTGGAGTCG CCCAGCAAGG GCGACGAGGA TCCCTTCAAA 
GCACTGAAGA CACTGGCCCT TGACTTGTCA TCTAAGTACA AGACCGATGG CTCTCGGATC 
GCCTGGGTGT CCATCCGGCT GCCGGAGCAG CTGAGCGAAC CGGTGGATCC GCAGAGCTGG 
CTGCTGCACG CCTCCATGTA TGGACCACGC CAGCTTCTCA GTCTGGTGGT TGCGGATCTG 
GCACTGCCCA AACTGGGCCT CGAATCCCTC GTGCTGCTGG CCACGCCAGG CATGGTCTTC 
AAGGCGGACT TTCTGAATCG CGTGCGCATG AACACGATAC AGGGCTTCCA GGTGTACGCA 
CCAATCGGCT TCCAGATGTA CCCATGCCGC TGGGCGCATT TCTGCCGTGA GTGCGACACC 
TGCGACGTGA GCCAGAGCAG CGGTTACTTC GATCGCCATA ACCACGACGT GATCGCTTTC 
TATAGTCGGG ATTATGTCCA GGCTCGCAAG CTCTTACATC CGCAGGGACT GCCGATCATA 
CGCAGCGACC TGGACATCGA CCAGCTGCTG CTGCAGCCTG GCGAGGAGAC GCGACCACCG 
GGCGTGGAAA GCATTCTCGA TATGTTCGTG GCGGCACAGC ACTCGGTGCA TATACTGCGC 
GGAGTGGAAC CGAATTTGCG CTTCGGCCAG GATGTGCGGA ATCACTTGGC ACGCGGAGGA 
ACGCTGCCGC AGAGCGTGCC GGAACGCTGC GGTCGGGAGC AGTGCATCCA CCTGGCGTCG 
CGCAAGCAGA TCGGCGACGC CATAATACGC TACGAGGACA AAAGTATTCT ACACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG43313 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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