Pde9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde9
Entrez Gene ID	32233
Full Name	Phosphodiesterase 9
Synonyms	CG1627,CG32648,CG3754,CG3761,CG3765,CG42276,Dmel\CG42276,Dmel_CG32648,Dmel_CG3754,PDE9
General protein information	Preferred Names Phosphodiesterase 9 Names CG42276 gene product from transcript CG42276-RF CG42276-PB CG42276-PD CG42276-PE CG42276-PF Pde9-PB Pde9-PD Pde9-PE Pde9-PF phosphodiesterase 9
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11C4-11D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272566.1	NP_001259495.1	phosphodiesterase 9, isoform E
NM_132606.5	NP_572834.2	phosphodiesterase 9, isoform B
NM_167340.5	NP_727644.3	phosphodiesterase 9, isoform D
NM_001272567.2	NP_001259496.1	phosphodiesterase 9, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila genome encodes five novel PDE genes in addition to dunce. Predicted PDE sequences of Drosophila show highly conserved critical domains when compared with human PDEs.
Title: Cyclic nucleotide phosphodiesterases in Drosophila melanogaster.

The following Pde9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01431	NM_132606.5 Latest version!	Drosophila melanogaster phosphodiesterase 9, transcript variant B (Pde9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa01431	NM_001272566.1 Latest version!	Drosophila melanogaster phosphodiesterase 9, transcript variant E (Pde9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa01431	NM_001272567.2 Latest version!	Drosophila melanogaster phosphodiesterase 9, transcript variant F (Pde9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OFa01432	NM_167340.5 Latest version!	Drosophila melanogaster phosphodiesterase 9, transcript variant D (Pde9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde9 ( NM_001272566.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259495.1 Drosophila melanogaster phosphodiesterase 9, transcript variant E (Pde9), mRNA.

Price & Availability↑

CloneID	OFa01431
Clone ID Related Accession (Same CDS sequence)	NM_001272566.1 , NM_132606.5 , NM_001272567.2
Accession Version	NM_001272566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphodiesterase 9, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259495.1
CDS	1249..5829
Translation	MYQDSGCSSSSSRRGSSSAAAATSTAATAAETAAAAAATTTSSSDEETLETLTIITTTITDSDIHATTTTTTVIASGTATTAAAAATTATLATLATLASNSDIEDSSPVESEDSEECEYIEIDCQTTAQEGTSPSGGSSSSGNAVLLQRSSNNLQQQQQQQQQQQQHLQLQLQQADERLLRKIGYIASRVRCLSKYYGDFRLVNPYSARRQRRQLQEILLRHSIFDPGKEHQRAIVLAAATAAIAGPLAAIDCVCGSSSISLEAVSSRSRSSELEDDHHEQDQVQEEQEQEQHQGDTEDNEEEQEHPSEKPERTDIEEEPPPAIAVTTTTTATVRRKSSSSSTISGTATVSGSVSVSASASQSFCSNNINLLEELLIQFYEEQDHRSNLTVIRHHHHQQHQQHQQQQRITNNNNNNNNCNSIQNNNNMSNPAATAAATATPSVEQPATSGTTNIHLQPTSLPDGSDNPRRRRASDCSAVQAALQNQLHCITLKNNNCGKAMPFHRGSCGAAGEHLLAANSIANRATIILSKSCSNVDGDATATAATALSSTVGGGIGGIGGSIKMRASATDIETNALNGARDAIVASNNGNNGNNGNNGNNEYSILQLNNTIIQCHFNDDDFRALVKDLKRKVEYTERMNWLCLSKRPLGPPHRKSSLPKHQEVKRRFLEICDTTFSEEVRAALRLPAFDSYEWSDADVIHLMQTMFVELGFIEKFSIPVDTLREWLYEVYKHYNEVPFHNFRHCFCVAQMMYAITRQANLLSRLGDLECLILLVSCICHDLDHPGYNNIYQINARTELALRYNDISPLENHHCSIAFRLLEHPECNIFKNFSRDTFNNIREGIIRCILATDMARHNEILTQFMEITPIFDYSNRAHINLLCMILIKVADISNEARPMDVAEPWLDRLLQEFFAQSAAEKSEGLPVTPFMDPDKVSKPGSQVRFIGLVLLPLFEALGELVPELTELIIIPVRIALEYYRRLNDAQTKTRKSVADSNTSATSDSNSGTIDSNAAMVSTPGGASDKLSLDKGQGNSQGSGGGGGGGGGGGAGGGTGSGCGSNAAGSVSPQMPRSGSGISVKSRRSIPSQKSASRTSVDEPGGMASELHDLPEGSESGDSETATEVDVAEKTSKFKVDTEGSSNRSKSSHSTSRKSSREKRPSMIGELCSSGGGQRIRNSYGNIHGYHSNRCHFGNNRAVSLDQYSSAGNNRRLSDGLPQVISDSNVFYGRHNRSSTETTVAVGNPQDTNANTNHPVGCQLKELLARTEADSDGEGDGNGREDKKIPLVIPSMPQLATSSNGNISPTLVVTEQILPSNGSTRSSASSGRGGSGVPGGSGGSGMPGPSAGSGSSWKSRLRQFSDYFSFSFDKSNKRFGSTRSSPCPGSNSSSGRTNNNANGLGENQDGLGAGGGIKPGMCCTTITNSSGSTVKGETRGGTAGAGGGALTTMTTGNDAHQRHRAYSLDVPGMMRYSSNDSSRHPSNNTLQSAGGGAGLTTGLEVTAQRVPPSLSVEMGLASGSSSEAGPKI

Target ORF information:

RefSeq Version	NM_001272566.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphodiesterase 9, transcript variant E (Pde9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272566.1

ORF Insert Sequence:

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde9 ( NM_132606.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572834.2 Drosophila melanogaster phosphodiesterase 9, transcript variant B (Pde9), mRNA.

Price & Availability↑

CloneID	OFa01431
Clone ID Related Accession (Same CDS sequence)	NM_001272566.1 , NM_132606.5 , NM_001272567.2
Accession Version	NM_132606.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphodiesterase 9, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132606.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572834.2
CDS	1384..5964
Translation	MYQDSGCSSSSSRRGSSSAAAATSTAATAAETAAAAAATTTSSSDEETLETLTIITTTITDSDIHATTTTTTVIASGTATTAAAAATTATLATLATLASNSDIEDSSPVESEDSEECEYIEIDCQTTAQEGTSPSGGSSSSGNAVLLQRSSNNLQQQQQQQQQQQQHLQLQLQQADERLLRKIGYIASRVRCLSKYYGDFRLVNPYSARRQRRQLQEILLRHSIFDPGKEHQRAIVLAAATAAIAGPLAAIDCVCGSSSISLEAVSSRSRSSELEDDHHEQDQVQEEQEQEQHQGDTEDNEEEQEHPSEKPERTDIEEEPPPAIAVTTTTTATVRRKSSSSSTISGTATVSGSVSVSASASQSFCSNNINLLEELLIQFYEEQDHRSNLTVIRHHHHQQHQQHQQQQRITNNNNNNNNCNSIQNNNNMSNPAATAAATATPSVEQPATSGTTNIHLQPTSLPDGSDNPRRRRASDCSAVQAALQNQLHCITLKNNNCGKAMPFHRGSCGAAGEHLLAANSIANRATIILSKSCSNVDGDATATAATALSSTVGGGIGGIGGSIKMRASATDIETNALNGARDAIVASNNGNNGNNGNNGNNEYSILQLNNTIIQCHFNDDDFRALVKDLKRKVEYTERMNWLCLSKRPLGPPHRKSSLPKHQEVKRRFLEICDTTFSEEVRAALRLPAFDSYEWSDADVIHLMQTMFVELGFIEKFSIPVDTLREWLYEVYKHYNEVPFHNFRHCFCVAQMMYAITRQANLLSRLGDLECLILLVSCICHDLDHPGYNNIYQINARTELALRYNDISPLENHHCSIAFRLLEHPECNIFKNFSRDTFNNIREGIIRCILATDMARHNEILTQFMEITPIFDYSNRAHINLLCMILIKVADISNEARPMDVAEPWLDRLLQEFFAQSAAEKSEGLPVTPFMDPDKVSKPGSQVRFIGLVLLPLFEALGELVPELTELIIIPVRIALEYYRRLNDAQTKTRKSVADSNTSATSDSNSGTIDSNAAMVSTPGGASDKLSLDKGQGNSQGSGGGGGGGGGGGAGGGTGSGCGSNAAGSVSPQMPRSGSGISVKSRRSIPSQKSASRTSVDEPGGMASELHDLPEGSESGDSETATEVDVAEKTSKFKVDTEGSSNRSKSSHSTSRKSSREKRPSMIGELCSSGGGQRIRNSYGNIHGYHSNRCHFGNNRAVSLDQYSSAGNNRRLSDGLPQVISDSNVFYGRHNRSSTETTVAVGNPQDTNANTNHPVGCQLKELLARTEADSDGEGDGNGREDKKIPLVIPSMPQLATSSNGNISPTLVVTEQILPSNGSTRSSASSGRGGSGVPGGSGGSGMPGPSAGSGSSWKSRLRQFSDYFSFSFDKSNKRFGSTRSSPCPGSNSSSGRTNNNANGLGENQDGLGAGGGIKPGMCCTTITNSSGSTVKGETRGGTAGAGGGALTTMTTGNDAHQRHRAYSLDVPGMMRYSSNDSSRHPSNNTLQSAGGGAGLTTGLEVTAQRVPPSLSVEMGLASGSSSEAGPKI

Target ORF information:

RefSeq Version	NM_132606.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphodiesterase 9, transcript variant B (Pde9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132606.5

ORF Insert Sequence:

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde9 ( NM_001272567.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259496.1 Drosophila melanogaster phosphodiesterase 9, transcript variant F (Pde9), mRNA.

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CloneID	OFa01431
Clone ID Related Accession (Same CDS sequence)	NM_001272566.1 , NM_132606.5 , NM_001272567.2
Accession Version	NM_001272567.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphodiesterase 9, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272567.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259496.1
CDS	1384..5964
Translation	MYQDSGCSSSSSRRGSSSAAAATSTAATAAETAAAAAATTTSSSDEETLETLTIITTTITDSDIHATTTTTTVIASGTATTAAAAATTATLATLATLASNSDIEDSSPVESEDSEECEYIEIDCQTTAQEGTSPSGGSSSSGNAVLLQRSSNNLQQQQQQQQQQQQHLQLQLQQADERLLRKIGYIASRVRCLSKYYGDFRLVNPYSARRQRRQLQEILLRHSIFDPGKEHQRAIVLAAATAAIAGPLAAIDCVCGSSSISLEAVSSRSRSSELEDDHHEQDQVQEEQEQEQHQGDTEDNEEEQEHPSEKPERTDIEEEPPPAIAVTTTTTATVRRKSSSSSTISGTATVSGSVSVSASASQSFCSNNINLLEELLIQFYEEQDHRSNLTVIRHHHHQQHQQHQQQQRITNNNNNNNNCNSIQNNNNMSNPAATAAATATPSVEQPATSGTTNIHLQPTSLPDGSDNPRRRRASDCSAVQAALQNQLHCITLKNNNCGKAMPFHRGSCGAAGEHLLAANSIANRATIILSKSCSNVDGDATATAATALSSTVGGGIGGIGGSIKMRASATDIETNALNGARDAIVASNNGNNGNNGNNGNNEYSILQLNNTIIQCHFNDDDFRALVKDLKRKVEYTERMNWLCLSKRPLGPPHRKSSLPKHQEVKRRFLEICDTTFSEEVRAALRLPAFDSYEWSDADVIHLMQTMFVELGFIEKFSIPVDTLREWLYEVYKHYNEVPFHNFRHCFCVAQMMYAITRQANLLSRLGDLECLILLVSCICHDLDHPGYNNIYQINARTELALRYNDISPLENHHCSIAFRLLEHPECNIFKNFSRDTFNNIREGIIRCILATDMARHNEILTQFMEITPIFDYSNRAHINLLCMILIKVADISNEARPMDVAEPWLDRLLQEFFAQSAAEKSEGLPVTPFMDPDKVSKPGSQVRFIGLVLLPLFEALGELVPELTELIIIPVRIALEYYRRLNDAQTKTRKSVADSNTSATSDSNSGTIDSNAAMVSTPGGASDKLSLDKGQGNSQGSGGGGGGGGGGGAGGGTGSGCGSNAAGSVSPQMPRSGSGISVKSRRSIPSQKSASRTSVDEPGGMASELHDLPEGSESGDSETATEVDVAEKTSKFKVDTEGSSNRSKSSHSTSRKSSREKRPSMIGELCSSGGGQRIRNSYGNIHGYHSNRCHFGNNRAVSLDQYSSAGNNRRLSDGLPQVISDSNVFYGRHNRSSTETTVAVGNPQDTNANTNHPVGCQLKELLARTEADSDGEGDGNGREDKKIPLVIPSMPQLATSSNGNISPTLVVTEQILPSNGSTRSSASSGRGGSGVPGGSGGSGMPGPSAGSGSSWKSRLRQFSDYFSFSFDKSNKRFGSTRSSPCPGSNSSSGRTNNNANGLGENQDGLGAGGGIKPGMCCTTITNSSGSTVKGETRGGTAGAGGGALTTMTTGNDAHQRHRAYSLDVPGMMRYSSNDSSRHPSNNTLQSAGGGAGLTTGLEVTAQRVPPSLSVEMGLASGSSSEAGPKI

Target ORF information:

RefSeq Version	NM_001272567.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphodiesterase 9, transcript variant F (Pde9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272567.2

ORF Insert Sequence:

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde9 ( NM_167340.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727644.3 Drosophila melanogaster phosphodiesterase 9, transcript variant D (Pde9), mRNA.

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CloneID	OFa01432
Clone ID Related Accession (Same CDS sequence)	NM_167340.5
Accession Version	NM_167340.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphodiesterase 9, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167340.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG ACAACAGGGG AGCACCAGGC AGGATGCGGT AGCCATTAGC 
AAGGGCTACG ACGGGGCCGT TGAGCAACAG GAGGAGGAGG AGGAGGAGGA GGATGAGCAG 
GAGGACGATG ACGAGGATGA GGGACGCGAG GAGCACCGCC AGCTGCAGCG GCATCAGCAG 
CAGCCGGACG CTTCGTCCAG CTGCGAGGTG TCCTCCATGT CGATGACCGT GTGGTCGGCG 
ATTGGTCAGC GTTTGAACGC CCTCGTCTGC CATTGCTGCG AACGCAAATT ACCGGAACCA 
GAAAATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727644.3
CDS	1384..6255
Translation	MYQDSGCSSSSSRRGSSSAAAATSTAATAAETAAAAAATTTSSSDEETLETLTIITTTITDSDIHATTTTTTVIASGTATTAAAAATTATLATLATLASNSDIEDSSPVESEDSEECEYIEIDCQTTAQEGTSPSGGSSSSGNAVLLQRSSNNLQQQQQQQQQQQQHLQLQLQQADERLLRKIGYIASRVRCLSKYYGDFRLVNPYSARRQRRQLQEILLRHSIFDPGKEHQRAIVLAAATAAIAGPLAAIDCVCGSSSISLEAVSSRSRSSELEDDHHEQDQVQEEQEQEQHQGDTEDNEEEQEHPSEKPERTDIEEEPPPAIAVTTTTTATVRRKSSSSSTISGTATVSGSVSVSASASQSFCSNNINLLEELLIQFYEEQDHRSNLTVIRHHHHQQHQQHQQQQRITNNNNNNNNCNSIQNNNNMSNPAATAAATATPSVEQPATSGTTNIHLQPTSLPDGSDNPRRRRASDCSAVQAALQNQLHCITLKNNNCGKAMPFHRGSCGAAGEHLLAANSIANRATIILSKSCSNVDGDATATAATALSSTVGGGIGGIGGSIKMRASATDIETNALNGARDAIVASNNGNNGNNGNNGNNEYSILQLNNTIIQCHFNDDDFRALVKDLKRKVEYTERMNWLCLSKRPLGPPHRKSSLPKHQEVKRRFLEICDTTFSEEVRAALRLPAFDSYEWSDADVIHLMQTMFVELGFIEKFSIPVDTLREWLYEVYKHYNEVPFHNFRHCFCVAQMMYAITRQANLLSRLGDLECLILLVSCICHDLDHPGYNNIYQINARTELALRYNDISPLENHHCSIAFRLLEHPECNIFKNFSRDTFNNIREGIIRCILATDMARHNEILTQFMEITPIFDYSNRAHINLLCMILIKVADISNEARPMDVAEPWLDRLLQEFFAQSAAEKSEGLPVTPFMDPDKVSKPGSQVRFIGLVLLPLFEALGELVPELTELIIIPVRIALEYYRRLNDAQTKTRKSVADSNTSATSDSNSGTIDSNAAMVSTPGGASDKLSLDKGQGNSQGSGGGGGGGGGGGAGGGTGSGCGSNAAGSVSPQMPRSGSGISVKSRRSIPSQKSASRTSVDEPGGMASELHDLPEGSESGDSETATEVDVAEKTSKFKVDTEGSSNRSKSSHSTSRKSSREKRPSMIGELCSSGGGQRIRNSYGNIHGYHSNRCHFGNNRAVSLDQYSSAGNNRRLSDGLPQVISDSNVFYGRHNRSSTETTVAVGNPQDTNANTNHPVGCQLKELLARTEADSDGEGDGNGREDKKIPLVIPSMPQLATSSNGNISPTLVVTEQILPSNGSTRSSASSGRGGSGVPGGSGGSGMPGPSAGSGSSWKSRLRQFSDYFSFSFDKSNKRFGSTRSSPCPGSNSSSGRTNNNANGLGENQDGLGAGGGIKPGMCCTTITNSSGSTVKGETRGGTAGAGGGALTTMTTGNDAHQRHRAYSLDVPGMMRYSSNDSSRHPSNNTLQSAGGGAGLTTGLEVTAQRVPPSLSVEMGLASGSSSEAGPKI*QQGSTRQDAVAISKGYDGAVEQQEEEEEEEDEQEDDDEDEGREEHRQLQRHQQQPDASSSCEVSSMSMTVWSAIGQRLNALVCHCCERKLPEPENV

Target ORF information:

RefSeq Version	NM_167340.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphodiesterase 9, transcript variant D (Pde9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167340.5

ORF Insert Sequence:

ATGTACCAAG ACAGCGGCTG CAGCAGCAGT AGCAGTCGAC GCGGCAGCAG CAGTGCAGCA 
GCAGCAACAT CTACAGCAGC AACTGCAGCA GAAACTGCAG CAGCAGCAGC AGCAACAACA 
ACCAGCAGCA GCGACGAGGA AACACTCGAG ACTCTGACGA TTATAACGAC CACCATCACC 
GATAGCGATA TCCATGCGAC CACAACAACT ACGACGGTTA TAGCCAGCGG TACGGCGACA 
ACAGCGGCAG CGGCAGCGAC AACAGCAACA CTTGCCACAC TTGCAACACT CGCCAGCAAC 
AGCGACATCG AGGACAGCAG TCCGGTGGAG AGCGAGGATA GCGAGGAGTG CGAGTATATC 
GAGATCGATT GCCAGACGAC CGCCCAGGAA GGCACTTCGC CAAGTGGCGG CAGCAGCAGC 
AGCGGCAATG CGGTCTTGCT GCAACGCAGC AGCAATAACC TCCAGCAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA TTTGCAACTG CAGCTGCAGC AGGCGGACGA GCGATTGTTG 
CGCAAAATCG GCTATATTGC ATCGCGTGTG CGCTGCCTGT CCAAGTACTA CGGTGACTTC 
CGCCTGGTGA ATCCGTACAG TGCCCGCCGG CAGAGGCGCC AATTGCAGGA GATCCTGCTG 
CGGCACTCGA TCTTCGATCC TGGCAAGGAG CACCAGCGGG CGATTGTCTT GGCCGCGGCA 
ACAGCGGCAA TTGCCGGTCC TCTGGCGGCC ATCGATTGCG TCTGCGGCAG CAGTAGCATC 
AGTTTAGAGG CAGTCAGCAG CAGAAGTCGC AGCAGTGAGC TGGAGGACGA TCATCACGAG 
CAGGATCAGG TGCAGGAGGA GCAGGAGCAG GAGCAGCATC AGGGGGATAC CGAGGATAAC 
GAAGAGGAGC AAGAGCATCC GAGTGAAAAG CCAGAGAGAA CAGACATTGA GGAGGAGCCA 
CCGCCGGCGA TTGCAGTGAC CACCACCACA ACGGCCACCG TGCGCCGGAA GTCCTCGAGC 
AGTTCCACAA TCAGTGGCAC GGCCACCGTT TCCGGTTCCG TTTCGGTATC GGCCTCCGCC 
TCTCAATCCT TTTGCTCCAA CAACATCAAT CTGCTCGAGG AGCTGCTCAT TCAGTTCTAC 
GAGGAGCAGG ATCATCGCAG CAACCTAACG GTGATCCGAC ATCATCATCA TCAGCAGCAC 
CAGCAGCACC AGCAGCAGCA AAGAATCACC AACAATAATA ACAACAATAA CAATTGCAAC 
AGCATACAGA ACAACAACAA CATGTCGAAT CCTGCGGCTA CAGCGGCAGC AACAGCAACG 
CCATCAGTGG AACAACCGGC AACATCGGGC ACCACCAACA TCCACTTGCA ACCCACCTCC 
CTGCCCGATG GCAGCGATAA TCCGCGCCGC CGCCGGGCCA GCGATTGCTC AGCGGTGCAG 
GCCGCTCTCC AGAATCAGCT GCACTGCATC ACGCTGAAGA ACAACAATTG CGGCAAGGCG 
ATGCCCTTCC ATCGCGGCAG TTGCGGCGCC GCCGGTGAGC ATCTGTTGGC CGCCAATTCG 
ATAGCTAATC GTGCCACCAT TATTTTGAGT AAATCATGCA GCAATGTGGA TGGTGATGCC 
ACTGCCACAG CGGCAACGGC ACTAAGTTCA ACGGTTGGCG GCGGCATCGG TGGCATTGGT 
GGCAGTATAA AAATGCGTGC CAGTGCCACA GATATCGAGA CGAATGCATT GAACGGTGCC 
CGGGATGCCA TTGTTGCATC GAATAACGGT AATAACGGCA ATAACGGAAA TAACGGGAAC 
AACGAGTACA GCATCCTGCA GCTGAACAAC ACGATCATCC AGTGCCACTT CAACGACGAT 
GACTTTCGCG CCCTGGTCAA GGACCTCAAG CGCAAGGTCG AGTACACGGA GCGCATGAAC 
TGGCTATGTC TCTCCAAACG ACCGCTGGGC CCGCCGCATC GTAAAAGCAG TCTACCAAAG 
CATCAGGAGG TGAAGCGTCG CTTCCTGGAA ATTTGTGATA CGACCTTCTC GGAGGAGGTG 
AGGGCCGCCC TACGCCTGCC GGCCTTCGAT TCCTACGAGT GGAGCGATGC CGATGTCATC 
CATCTCATGC AGACCATGTT CGTCGAGCTG GGATTCATTG AGAAGTTCAG CATTCCGGTG 
GACACGCTGC GCGAGTGGCT CTATGAGGTC TACAAGCACT ACAACGAGGT GCCCTTCCAC 
AACTTTCGGC ACTGCTTCTG CGTTGCCCAG ATGATGTATG CCATAACGCG CCAGGCGAAC 
TTGCTGAGTC GCCTGGGCGA CCTAGAGTGT CTGATCCTGT TGGTGTCCTG CATTTGCCAC 
GACCTCGACC ATCCTGGCTA CAATAATATC TACCAGATCA ATGCCCGCAC CGAGCTGGCA 
CTCAGATATA ATGACATCTC ACCGCTGGAG AATCACCATT GTTCGATAGC GTTCCGCCTG 
CTGGAACATC CCGAATGCAA TATATTCAAG AACTTTAGCC GCGACACCTT CAATAACATT 
CGCGAGGGCA TCATCCGGTG CATTTTGGCC ACGGACATGG CCCGTCACAA TGAAATCCTC 
ACGCAGTTTA TGGAGATAAC GCCCATCTTC GACTATAGCA ATCGTGCCCA CATCAATTTG 
CTCTGCATGA TCCTGATCAA AGTGGCGGAC ATTTCGAACG AGGCGCGACC CATGGACGTG 
GCCGAGCCCT GGCTGGACCG CCTGCTCCAG GAGTTCTTTG CCCAGAGCGC AGCCGAGAAG 
TCCGAGGGAT TGCCGGTGAC GCCGTTCATG GATCCGGACA AGGTCAGCAA GCCGGGCTCG 
CAGGTGCGCT TCATCGGACT CGTTCTCCTG CCACTATTCG AGGCTCTAGG CGAACTGGTG 
CCGGAGCTAA CCGAGCTCAT CATCATACCC GTGCGCATCG CCCTAGAGTA CTACAGGCGC 
CTCAACGATG CCCAGACAAA AACGCGCAAA TCGGTGGCGG ACAGCAATAC GTCCGCAACC 
TCCGATAGCA ACAGCGGCAC CATCGATTCC AATGCGGCGA TGGTCAGCAC ACCGGGAGGC 
GCCAGCGATA AGCTCAGCTT GGACAAGGGA CAGGGCAATT CGCAGGGCTC CGGCGGCGGA 
GGAGGCGGAG GAGGAGGAGG AGGTGCCGGT GGTGGGACAG GATCTGGATG CGGCAGCAAT 
GCCGCTGGTA GCGTATCGCC ACAAATGCCA CGCTCCGGAT CCGGGATCAG TGTTAAGTCG 
CGACGCAGCA TTCCATCGCA AAAGTCCGCC TCGAGGACGT CCGTGGATGA GCCCGGAGGA 
ATGGCGTCGG AGCTCCACGA TTTGCCGGAG GGCAGCGAGA GCGGTGATTC GGAAACGGCC 
ACCGAAGTTG ATGTGGCCGA GAAGACGTCC AAGTTCAAGG TGGACACCGA GGGCAGCAGC 
AACCGGAGCA AGTCCTCGCA CTCGACCTCC CGGAAGTCCT CTCGCGAGAA GCGTCCCTCG 
ATGATCGGTG AACTGTGCAG CAGTGGCGGT GGCCAGCGAA TCCGGAACTC GTACGGCAAC 
ATCCACGGCT ATCACAGCAA TCGCTGTCAT TTTGGCAACA ATCGGGCGGT TAGCCTGGAT 
CAGTATAGTA GTGCCGGTAA CAATCGCCGC CTCTCGGACG GACTGCCGCA GGTGATCTCG 
GACAGCAATG TTTTCTACGG CCGGCACAAT CGGAGCAGCA CGGAGACCAC TGTTGCTGTT 
GGCAATCCGC AGGATACCAA TGCGAATACC AATCATCCGG TCGGATGTCA GCTGAAAGAG 
CTATTGGCCC GCACCGAAGC GGACTCGGAT GGTGAGGGCG ATGGCAATGG CAGAGAGGAC 
AAAAAGATTC CATTGGTCAT ACCGTCGATG CCGCAGCTGG CCACATCATC GAACGGTAAC 
ATATCGCCGA CACTGGTGGT CACCGAGCAA ATCCTGCCCA GCAACGGCAG TACACGGAGC 
AGCGCCAGCA GTGGCCGTGG CGGCTCCGGC GTACCTGGAG GATCGGGTGG CTCTGGGATG 
CCTGGACCAT CGGCCGGTTC TGGTAGTTCA TGGAAATCGA GACTGCGCCA GTTCTCCGAT 
TACTTTAGCT TCAGCTTTGA TAAGAGCAAC AAGCGCTTTG GCAGCACACG TAGTAGTCCG 
TGTCCCGGTT CCAATTCCAG TTCGGGACGG ACCAATAACA ATGCCAATGG ATTGGGTGAA 
AATCAGGACG GATTGGGAGC AGGAGGCGGT ATAAAGCCGG GCATGTGCTG CACCACCATC 
ACCAATAGCT CAGGATCCAC GGTAAAAGGC GAGACGAGAG GAGGTACCGC CGGTGCAGGC 
GGCGGTGCTC TGACCACCAT GACCACCGGA AATGATGCCC ATCAACGCCA TCGCGCCTAT 
AGCCTGGATG TGCCCGGCAT GATGAGATAC TCCTCCAACG ACAGCAGTCG CCATCCCAGC 
AACAATACGC TTCAGAGTGC GGGTGGCGGT GCTGGTTTGA CCACCGGATT AGAGGTCACT 
GCCCAACGAG TGCCGCCCAG TTTGAGCGTT GAAATGGGCC TGGCCAGCGG CTCATCATCA 
GAGGCGGGCC CAAAGATTTG ACAACAGGGG AGCACCAGGC AGGATGCGGT AGCCATTAGC 
AAGGGCTACG ACGGGGCCGT TGAGCAACAG GAGGAGGAGG AGGAGGAGGA GGATGAGCAG 
GAGGACGATG ACGAGGATGA GGGACGCGAG GAGCACCGCC AGCTGCAGCG GCATCAGCAG 
CAGCCGGACG CTTCGTCCAG CTGCGAGGTG TCCTCCATGT CGATGACCGT GTGGTCGGCG 
ATTGGTCAGC GTTTGAACGC CCTCGTCTGC CATTGCTGCG AACGCAAATT ACCGGAACCA 
GAAAATGTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pde9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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