Agpat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Agpat1
Entrez Gene ID	32230
Full Name	CG3812 gene product from transcript CG3812-RB
Synonyms	AGPAT1,AGPAT2,Dmel\CG3812
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11B16-11C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272565.1	NP_001259494.1	1-Acylglycerol-3-phosphate O-acyltransferase 1, isoform C
NM_167337.2	NP_727641.1	1-Acylglycerol-3-phosphate O-acyltransferase 1, isoform B
NM_132600.2	NP_572828.1	1-Acylglycerol-3-phosphate O-acyltransferase 1, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AGPAT1	XP_001163919.2
Drosophila melanogaster (fruit fly)	CG3812	NP_727641.1
Bos taurus (cattle)	AGPAT1	NP_803484.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002021	XP_321034.5
Xenopus tropicalis (tropical clawed frog)	agpat1	NP_001090802.1
Homo sapiens (human)	AGPAT1	NP_116130.2
Canis lupus familiaris (dog)	AGPAT1	XP_005627233.1
Danio rerio (zebrafish)	agpat2	NP_001071200.1
Caenorhabditis elegans (roundworm)	acl-2	NP_001256175.1
	AGPAT1	XP_001114674.1
Mus musculus (house mouse)	Agpat1	NP_061350.3
Rattus norvegicus (Norway rat)	Agpat1	NP_997623.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Agpat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Agpat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01430	NM_132600.2 Latest version!	Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01430	NM_001272565.1 Latest version!	Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01430	NM_167337.2 Latest version!	Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Agpat1 ( NM_001272565.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259494.1 Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01430
Clone ID Related Accession (Same CDS sequence)	NM_001272565.1 , NM_167337.2 , NM_132600.2
Accession Version	NM_001272565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	1-Acylglycerol-3-phosphate O-acyltransferase 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCACCA TGACTTCGTT TATCGAACTG CTGGGACTTT TCTTGCTGCT GATGCTGCCA 
TTCCTCTATG AGACCAATCA CATATTCCGA TACTACTTCA AATTCCTGAT GTACTACGGC 
ATCGTGTCGT TCAACTCGAT CATCCTCATC CCGGCATTCC TCACCAGACC ATGCGATGTC 
CGCAATCTGC TATGGGCGAG CACCTGGTGC CACCGCGTTT CGACGTTGAT TGGCCTGCGA 
TGGGAGCTGC GCGGCAAGGA GCATTTGGCC AAGGACCAGG CCTGCATCAT TGTGGCCAAT 
CACCAAAGCT CGCTGGATGT GCTGGGCATG TTCAACATCT GGCATGTGAT GAACAAGTGC 
ACAGTGGTGG CCAAACGAGA GCTCTTCTAC GCCTGGCCTT TTGGCTTGGC GGCATGGTTG 
GCTGGCCTAA TCTTCATCGA TCGTGTGCGC GGCGAGAAGG CGCGCGAAAC GCTAAACGAT 
GTTAACCGGC GCATCAAGAA GCAACGTATC AAACTGTGGG TTTTCCCGGA GGGAACGCGC 
CGCAATACGG GCGCACTGCA TCCGTTCAAA AAGGGCGCCT TTCACATGGC CATCGATCAG 
CAGATACCCA TTCTGCCGGT GGTCTTCTCC TCCTACTGCA CATTCCTCAA TGATAAGAAA 
AAGATTTTGA ATTCGGGTCG CATTGTGATT ACCACGCTGC CGCCGGTTAG CACCGAAGGA 
CTAACCAAGG ACGACATCGA TGTGCTAATG GAGCGTGTCC GCTCCCAGAT GATTGAAACG 
TTCAAGGTGA CGTCTGCGGA GGCGTTGCAT CGTTATAAGC CCATTAAAAA GGTTGGAATT 
CCAGCCGCCT CATCAGCATC AACATCATCT ACGACAACAA CAACATTGGC ATCACCGGGA 
ACTGCTGTCA CTCCCGTTGC GGATGCGGCA GTGGCTGCCG CAGCTGCCGT GGCCAGTAGC 
AGTTCGGCAT ACGGTCAACC GCCGGGCTAC GAAGCCGGAG CCGCAAAGGC CAGCTTGCTC 
AAGACACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259494.1
CDS	305..1336
Translation	MITMTSFIELLGLFLLLMLPFLYETNHIFRYYFKFLMYYGIVSFNSIILIPAFLTRPCDVRNLLWASTWCHRVSTLIGLRWELRGKEHLAKDQACIIVANHQSSLDVLGMFNIWHVMNKCTVVAKRELFYAWPFGLAAWLAGLIFIDRVRGEKARETLNDVNRRIKKQRIKLWVFPEGTRRNTGALHPFKKGAFHMAIDQQIPILPVVFSSYCTFLNDKKKILNSGRIVITTLPPVSTEGLTKDDIDVLMERVRSQMIETFKVTSAEALHRYKPIKKVGIPAASSASTSSTTTTTLASPGTAVTPVADAAVAAAAAVASSSSAYGQPPGYEAGAAKASLLKTL

Target ORF information:

RefSeq Version	NM_001272565.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272565.1

ORF Insert Sequence:

ATGATCACCA TGACTTCGTT TATCGAACTG CTGGGACTTT TCTTGCTGCT GATGCTGCCA 
TTCCTCTATG AGACCAATCA CATATTCCGA TACTACTTCA AATTCCTGAT GTACTACGGC 
ATCGTGTCGT TCAACTCGAT CATCCTCATC CCGGCATTCC TCACCAGACC ATGCGATGTC 
CGCAATCTGC TATGGGCGAG CACCTGGTGC CACCGCGTTT CGACGTTGAT TGGCCTGCGA 
TGGGAGCTGC GCGGCAAGGA GCATTTGGCC AAGGACCAGG CCTGCATCAT TGTGGCCAAT 
CACCAAAGCT CGCTGGATGT GCTGGGCATG TTCAACATCT GGCATGTGAT GAACAAGTGC 
ACAGTGGTGG CCAAACGAGA GCTCTTCTAC GCCTGGCCTT TTGGCTTGGC GGCATGGTTG 
GCTGGCCTAA TCTTCATCGA TCGTGTGCGC GGCGAGAAGG CGCGCGAAAC GCTAAACGAT 
GTTAACCGGC GCATCAAGAA GCAACGTATC AAACTGTGGG TTTTCCCGGA GGGAACGCGC 
CGCAATACGG GCGCACTGCA TCCGTTCAAA AAGGGCGCCT TTCACATGGC CATCGATCAG 
CAGATACCCA TTCTGCCGGT GGTCTTCTCC TCCTACTGCA CATTCCTCAA TGATAAGAAA 
AAGATTTTGA ATTCGGGTCG CATTGTGATT ACCACGCTGC CGCCGGTTAG CACCGAAGGA 
CTAACCAAGG ACGACATCGA TGTGCTAATG GAGCGTGTCC GCTCCCAGAT GATTGAAACG 
TTCAAGGTGA CGTCTGCGGA GGCGTTGCAT CGTTATAAGC CCATTAAAAA GGTTGGAATT 
CCAGCCGCCT CATCAGCATC AACATCATCT ACGACAACAA CAACATTGGC ATCACCGGGA 
ACTGCTGTCA CTCCCGTTGC GGATGCGGCA GTGGCTGCCG CAGCTGCCGT GGCCAGTAGC 
AGTTCGGCAT ACGGTCAACC GCCGGGCTAC GAAGCCGGAG CCGCAAAGGC CAGCTTGCTC 
AAGACACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agpat1 ( NM_167337.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727641.1 Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01430
Clone ID Related Accession (Same CDS sequence)	NM_001272565.1 , NM_167337.2 , NM_132600.2
Accession Version	NM_167337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	1-Acylglycerol-3-phosphate O-acyltransferase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167337.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCACCA TGACTTCGTT TATCGAACTG CTGGGACTTT TCTTGCTGCT GATGCTGCCA 
TTCCTCTATG AGACCAATCA CATATTCCGA TACTACTTCA AATTCCTGAT GTACTACGGC 
ATCGTGTCGT TCAACTCGAT CATCCTCATC CCGGCATTCC TCACCAGACC ATGCGATGTC 
CGCAATCTGC TATGGGCGAG CACCTGGTGC CACCGCGTTT CGACGTTGAT TGGCCTGCGA 
TGGGAGCTGC GCGGCAAGGA GCATTTGGCC AAGGACCAGG CCTGCATCAT TGTGGCCAAT 
CACCAAAGCT CGCTGGATGT GCTGGGCATG TTCAACATCT GGCATGTGAT GAACAAGTGC 
ACAGTGGTGG CCAAACGAGA GCTCTTCTAC GCCTGGCCTT TTGGCTTGGC GGCATGGTTG 
GCTGGCCTAA TCTTCATCGA TCGTGTGCGC GGCGAGAAGG CGCGCGAAAC GCTAAACGAT 
GTTAACCGGC GCATCAAGAA GCAACGTATC AAACTGTGGG TTTTCCCGGA GGGAACGCGC 
CGCAATACGG GCGCACTGCA TCCGTTCAAA AAGGGCGCCT TTCACATGGC CATCGATCAG 
CAGATACCCA TTCTGCCGGT GGTCTTCTCC TCCTACTGCA CATTCCTCAA TGATAAGAAA 
AAGATTTTGA ATTCGGGTCG CATTGTGATT ACCACGCTGC CGCCGGTTAG CACCGAAGGA 
CTAACCAAGG ACGACATCGA TGTGCTAATG GAGCGTGTCC GCTCCCAGAT GATTGAAACG 
TTCAAGGTGA CGTCTGCGGA GGCGTTGCAT CGTTATAAGC CCATTAAAAA GGTTGGAATT 
CCAGCCGCCT CATCAGCATC AACATCATCT ACGACAACAA CAACATTGGC ATCACCGGGA 
ACTGCTGTCA CTCCCGTTGC GGATGCGGCA GTGGCTGCCG CAGCTGCCGT GGCCAGTAGC 
AGTTCGGCAT ACGGTCAACC GCCGGGCTAC GAAGCCGGAG CCGCAAAGGC CAGCTTGCTC 
AAGACACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727641.1
CDS	534..1565
Translation	MITMTSFIELLGLFLLLMLPFLYETNHIFRYYFKFLMYYGIVSFNSIILIPAFLTRPCDVRNLLWASTWCHRVSTLIGLRWELRGKEHLAKDQACIIVANHQSSLDVLGMFNIWHVMNKCTVVAKRELFYAWPFGLAAWLAGLIFIDRVRGEKARETLNDVNRRIKKQRIKLWVFPEGTRRNTGALHPFKKGAFHMAIDQQIPILPVVFSSYCTFLNDKKKILNSGRIVITTLPPVSTEGLTKDDIDVLMERVRSQMIETFKVTSAEALHRYKPIKKVGIPAASSASTSSTTTTTLASPGTAVTPVADAAVAAAAAVASSSSAYGQPPGYEAGAAKASLLKTL

Target ORF information:

RefSeq Version	NM_167337.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167337.2

ORF Insert Sequence:

ATGATCACCA TGACTTCGTT TATCGAACTG CTGGGACTTT TCTTGCTGCT GATGCTGCCA 
TTCCTCTATG AGACCAATCA CATATTCCGA TACTACTTCA AATTCCTGAT GTACTACGGC 
ATCGTGTCGT TCAACTCGAT CATCCTCATC CCGGCATTCC TCACCAGACC ATGCGATGTC 
CGCAATCTGC TATGGGCGAG CACCTGGTGC CACCGCGTTT CGACGTTGAT TGGCCTGCGA 
TGGGAGCTGC GCGGCAAGGA GCATTTGGCC AAGGACCAGG CCTGCATCAT TGTGGCCAAT 
CACCAAAGCT CGCTGGATGT GCTGGGCATG TTCAACATCT GGCATGTGAT GAACAAGTGC 
ACAGTGGTGG CCAAACGAGA GCTCTTCTAC GCCTGGCCTT TTGGCTTGGC GGCATGGTTG 
GCTGGCCTAA TCTTCATCGA TCGTGTGCGC GGCGAGAAGG CGCGCGAAAC GCTAAACGAT 
GTTAACCGGC GCATCAAGAA GCAACGTATC AAACTGTGGG TTTTCCCGGA GGGAACGCGC 
CGCAATACGG GCGCACTGCA TCCGTTCAAA AAGGGCGCCT TTCACATGGC CATCGATCAG 
CAGATACCCA TTCTGCCGGT GGTCTTCTCC TCCTACTGCA CATTCCTCAA TGATAAGAAA 
AAGATTTTGA ATTCGGGTCG CATTGTGATT ACCACGCTGC CGCCGGTTAG CACCGAAGGA 
CTAACCAAGG ACGACATCGA TGTGCTAATG GAGCGTGTCC GCTCCCAGAT GATTGAAACG 
TTCAAGGTGA CGTCTGCGGA GGCGTTGCAT CGTTATAAGC CCATTAAAAA GGTTGGAATT 
CCAGCCGCCT CATCAGCATC AACATCATCT ACGACAACAA CAACATTGGC ATCACCGGGA 
ACTGCTGTCA CTCCCGTTGC GGATGCGGCA GTGGCTGCCG CAGCTGCCGT GGCCAGTAGC 
AGTTCGGCAT ACGGTCAACC GCCGGGCTAC GAAGCCGGAG CCGCAAAGGC CAGCTTGCTC 
AAGACACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agpat1 ( NM_132600.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572828.1 Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01430
Clone ID Related Accession (Same CDS sequence)	NM_001272565.1 , NM_167337.2 , NM_132600.2
Accession Version	NM_132600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	1-Acylglycerol-3-phosphate O-acyltransferase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132600.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCACCA TGACTTCGTT TATCGAACTG CTGGGACTTT TCTTGCTGCT GATGCTGCCA 
TTCCTCTATG AGACCAATCA CATATTCCGA TACTACTTCA AATTCCTGAT GTACTACGGC 
ATCGTGTCGT TCAACTCGAT CATCCTCATC CCGGCATTCC TCACCAGACC ATGCGATGTC 
CGCAATCTGC TATGGGCGAG CACCTGGTGC CACCGCGTTT CGACGTTGAT TGGCCTGCGA 
TGGGAGCTGC GCGGCAAGGA GCATTTGGCC AAGGACCAGG CCTGCATCAT TGTGGCCAAT 
CACCAAAGCT CGCTGGATGT GCTGGGCATG TTCAACATCT GGCATGTGAT GAACAAGTGC 
ACAGTGGTGG CCAAACGAGA GCTCTTCTAC GCCTGGCCTT TTGGCTTGGC GGCATGGTTG 
GCTGGCCTAA TCTTCATCGA TCGTGTGCGC GGCGAGAAGG CGCGCGAAAC GCTAAACGAT 
GTTAACCGGC GCATCAAGAA GCAACGTATC AAACTGTGGG TTTTCCCGGA GGGAACGCGC 
CGCAATACGG GCGCACTGCA TCCGTTCAAA AAGGGCGCCT TTCACATGGC CATCGATCAG 
CAGATACCCA TTCTGCCGGT GGTCTTCTCC TCCTACTGCA CATTCCTCAA TGATAAGAAA 
AAGATTTTGA ATTCGGGTCG CATTGTGATT ACCACGCTGC CGCCGGTTAG CACCGAAGGA 
CTAACCAAGG ACGACATCGA TGTGCTAATG GAGCGTGTCC GCTCCCAGAT GATTGAAACG 
TTCAAGGTGA CGTCTGCGGA GGCGTTGCAT CGTTATAAGC CCATTAAAAA GGTTGGAATT 
CCAGCCGCCT CATCAGCATC AACATCATCT ACGACAACAA CAACATTGGC ATCACCGGGA 
ACTGCTGTCA CTCCCGTTGC GGATGCGGCA GTGGCTGCCG CAGCTGCCGT GGCCAGTAGC 
AGTTCGGCAT ACGGTCAACC GCCGGGCTAC GAAGCCGGAG CCGCAAAGGC CAGCTTGCTC 
AAGACACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572828.1
CDS	443..1474
Translation	MITMTSFIELLGLFLLLMLPFLYETNHIFRYYFKFLMYYGIVSFNSIILIPAFLTRPCDVRNLLWASTWCHRVSTLIGLRWELRGKEHLAKDQACIIVANHQSSLDVLGMFNIWHVMNKCTVVAKRELFYAWPFGLAAWLAGLIFIDRVRGEKARETLNDVNRRIKKQRIKLWVFPEGTRRNTGALHPFKKGAFHMAIDQQIPILPVVFSSYCTFLNDKKKILNSGRIVITTLPPVSTEGLTKDDIDVLMERVRSQMIETFKVTSAEALHRYKPIKKVGIPAASSASTSSTTTTTLASPGTAVTPVADAAVAAAAAVASSSSAYGQPPGYEAGAAKASLLKTL

Target ORF information:

RefSeq Version	NM_132600.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster 1-Acylglycerol-3-phosphate O-acyltransferase 1 (Agpat1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132600.2

ORF Insert Sequence:

ATGATCACCA TGACTTCGTT TATCGAACTG CTGGGACTTT TCTTGCTGCT GATGCTGCCA 
TTCCTCTATG AGACCAATCA CATATTCCGA TACTACTTCA AATTCCTGAT GTACTACGGC 
ATCGTGTCGT TCAACTCGAT CATCCTCATC CCGGCATTCC TCACCAGACC ATGCGATGTC 
CGCAATCTGC TATGGGCGAG CACCTGGTGC CACCGCGTTT CGACGTTGAT TGGCCTGCGA 
TGGGAGCTGC GCGGCAAGGA GCATTTGGCC AAGGACCAGG CCTGCATCAT TGTGGCCAAT 
CACCAAAGCT CGCTGGATGT GCTGGGCATG TTCAACATCT GGCATGTGAT GAACAAGTGC 
ACAGTGGTGG CCAAACGAGA GCTCTTCTAC GCCTGGCCTT TTGGCTTGGC GGCATGGTTG 
GCTGGCCTAA TCTTCATCGA TCGTGTGCGC GGCGAGAAGG CGCGCGAAAC GCTAAACGAT 
GTTAACCGGC GCATCAAGAA GCAACGTATC AAACTGTGGG TTTTCCCGGA GGGAACGCGC 
CGCAATACGG GCGCACTGCA TCCGTTCAAA AAGGGCGCCT TTCACATGGC CATCGATCAG 
CAGATACCCA TTCTGCCGGT GGTCTTCTCC TCCTACTGCA CATTCCTCAA TGATAAGAAA 
AAGATTTTGA ATTCGGGTCG CATTGTGATT ACCACGCTGC CGCCGGTTAG CACCGAAGGA 
CTAACCAAGG ACGACATCGA TGTGCTAATG GAGCGTGTCC GCTCCCAGAT GATTGAAACG 
TTCAAGGTGA CGTCTGCGGA GGCGTTGCAT CGTTATAAGC CCATTAAAAA GGTTGGAATT 
CCAGCCGCCT CATCAGCATC AACATCATCT ACGACAACAA CAACATTGGC ATCACCGGGA 
ACTGCTGTCA CTCCCGTTGC GGATGCGGCA GTGGCTGCCG CAGCTGCCGT GGCCAGTAGC 
AGTTCGGCAT ACGGTCAACC GCCGGGCTAC GAAGCCGGAG CCGCAAAGGC CAGCTTGCTC 
AAGACACTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Agpat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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