CkIalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CkIalpha
Entrez Gene ID	32221
Full Name	Casein kinase Ialpha
Synonyms	CG2028,CK I,CK1,CK1a,CK1alpha,CKI,CKI alpha,CKIa,CKIalpha,Ck1alpha,CkI alpha,CkIa,Dmel\CG2028,PKA-C,anon-WO03040301.93,anon-WO03040301.95,ck1a,ck1alpha,dmCK1,dmckI,l(1)G0492
General protein information	Preferred Names Casein kinase Ialpha Names CG2028 gene product from transcript CG2028-RE CG2028-PA CG2028-PB CG2028-PD CG2028-PE CG2028-PF CG2028-PG CkIalpha-PA CkIalpha-PB CkIalpha-PD CkIalpha-PE CkIalpha-PF CkIalpha-PG caesin kinase I alpha casein kinase 1 casein kinase 1a casein kinase 1alpha casein kinase I casein kinase ialpha casein kinase-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11B7-11B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169266.3	NP_001162737.1	casein kinase ialpha, isoform D
NM_001298235.1	NP_001285164.1	casein kinase ialpha, isoform F
NM_167332.3	NP_727632.1	casein kinase ialpha, isoform G
NM_078585.6	NP_511140.1	casein kinase ialpha, isoform B
NM_167331.4	NP_727631.1	casein kinase ialpha, isoform A
NM_001298234.1	NP_001285163.1	casein kinase ialpha, isoform E

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CSNK1A1	NP_777136.1
Gallus gallus (chicken)	CSNK1A1	NP_990384.1
Arabidopsis thaliana (thale cress)	ckl8	NP_199146.1
Arabidopsis thaliana (thale cress)	ADK1	NP_563695.2
Canis lupus familiaris (dog)	CSNK1A1	XP_005619366.1
Schizosaccharomyces pombe (fission yeast)	hhp2	NP_593184.1
Oryza sativa (rice)	Os02g0810400	NP_001048470.1
Oryza sativa (rice)	Os04g0514800	NP_001053309.1
Xenopus tropicalis (tropical clawed frog)	csnk1a1	NP_001001221.1
	CSNK1A1	XP_001106904.1
Rattus norvegicus (Norway rat)	Csnk1a1	NP_446067.1
Danio rerio (zebrafish)	LOC100334765	XP_002664490.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000627	XP_001238805.2
Arabidopsis thaliana (thale cress)	CKL13	NP_171939.1
Arabidopsis thaliana (thale cress)	CKI1	NP_193170.1
Arabidopsis thaliana (thale cress)	ckl7	NP_199223.1
Pan troglodytes (chimpanzee)	CSNK1A1	XP_001163959.1
Drosophila melanogaster (fruit fly)	CkIalpha	NP_001162737.1
Arabidopsis thaliana (thale cress)	ckl10	NP_188976.1
Oryza sativa (rice)	Os02g0622100	NP_001047465.1
Homo sapiens (human)	CSNK1A1	NP_001020276.1
Mus musculus (house mouse)	Csnk1a1	NP_666199.1
Arabidopsis thaliana (thale cress)	CKL6	NP_567812.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

glucose regulatory role
Title: Drosophila glucome screening identifies Ck1alpha as a regulator of mammalian glucose metabolism.

we identify Casein Kinase I alpha (CK1alpha) as an additional negative-regulator of Cap-H2. CK1a-depletion stabilizes Cap-H2 protein and results in an accumulation of Cap-H2 on chromosomes.
Title: Drosophila casein kinase I alpha regulates homolog pairing and genome organization by modulating condensin II subunit Cap-H2 levels.

Expected genes in the Scar-Arp2/3 actin nucleation pathway were identified as well as casein kinase I, which had a similar morphological RNAi signature.
Title: A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis.

Mutation of ck1alpha or -epsilon or sgg/gsk3beta in wing imaginal discs results in the accumulation of dMyc protein, suggesting a physiological role for these kinases in vivo.
Title: Identification of domains responsible for ubiquitin-dependent degradation of dMyc by glycogen synthase kinase 3beta and casein kinase 1 kinases.

Several CKI isoforms including CKIalpha and Gish/CKIgamma can phosphorylate the Wg coreceptor Arrow (Arr), which may account, at least in part, for their positive roles in the Wg pathway.
Title: Regulation of wingless signaling by the CKI family in Drosophila limb development.

The following CkIalpha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CkIalpha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01421	NM_001169266.3 Latest version!	Drosophila melanogaster casein kinase ialpha, transcript variant D (CkIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01421	NM_001298235.1 Latest version!	Drosophila melanogaster casein kinase ialpha, transcript variant F (CkIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01421	NM_078585.6 Latest version!	Drosophila melanogaster casein kinase ialpha, transcript variant B (CkIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01421	NM_167332.3 Latest version!	Drosophila melanogaster casein kinase ialpha, transcript variant G (CkIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01421	NM_001298234.1 Latest version!	Drosophila melanogaster casein kinase ialpha, transcript variant E (CkIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa01421	NM_167331.4 Latest version!	Drosophila melanogaster casein kinase ialpha, transcript variant A (CkIalpha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CkIalpha ( NM_001169266.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162737.1 Drosophila melanogaster casein kinase ialpha, transcript variant D (CkIalpha), mRNA.

Price & Availability↑

CloneID	OFa01421
Clone ID Related Accession (Same CDS sequence)	NM_001169266.3 , NM_001298235.1 , NM_167332.3 , NM_078585.6 , NM_167331.4 , NM_001298234.1
Accession Version	NM_001169266.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase ialpha, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169266.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162737.1
CDS	437..1450
Translation	MDKMRILKESRPEIIVGGKYRVIRKIGSGSFGDIYLGMSIQSGEEVAIKMESAHARHPQLLYEAKLYRILSGGVGFPRIRHHGKEKNFNTLVMDLLGPSLEDLFNFCTRHFTIKTVLMLVDQMIGRLEYIHLKCFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKFRDPHTRHHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGVLPWQGMKANTKQQKYEKISEKKMSTPIEVLCKGSPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRILFRTLNHQYDYIYDWTMLKQKTHQGQPNPAILLEQLDKDKEKQNGKPLIAD

Target ORF information:

RefSeq Version	NM_001169266.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase ialpha, transcript variant D (CkIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169266.3

ORF Insert Sequence:

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIalpha ( NM_001298235.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285164.1 Drosophila melanogaster casein kinase ialpha, transcript variant F (CkIalpha), mRNA.

Price & Availability↑

CloneID	OFa01421
Clone ID Related Accession (Same CDS sequence)	NM_001169266.3 , NM_001298235.1 , NM_167332.3 , NM_078585.6 , NM_167331.4 , NM_001298234.1
Accession Version	NM_001298235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase ialpha, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285164.1
CDS	202..1215
Translation	MDKMRILKESRPEIIVGGKYRVIRKIGSGSFGDIYLGMSIQSGEEVAIKMESAHARHPQLLYEAKLYRILSGGVGFPRIRHHGKEKNFNTLVMDLLGPSLEDLFNFCTRHFTIKTVLMLVDQMIGRLEYIHLKCFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKFRDPHTRHHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGVLPWQGMKANTKQQKYEKISEKKMSTPIEVLCKGSPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRILFRTLNHQYDYIYDWTMLKQKTHQGQPNPAILLEQLDKDKEKQNGKPLIAD

Target ORF information:

RefSeq Version	NM_001298235.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase ialpha, transcript variant F (CkIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298235.1

ORF Insert Sequence:

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIalpha ( NM_167332.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727632.1 Drosophila melanogaster casein kinase ialpha, transcript variant G (CkIalpha), mRNA.

Price & Availability↑

CloneID	OFa01421
Clone ID Related Accession (Same CDS sequence)	NM_001169266.3 , NM_001298235.1 , NM_167332.3 , NM_078585.6 , NM_167331.4 , NM_001298234.1
Accession Version	NM_167332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase ialpha, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167332.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727632.1
CDS	202..1215
Translation	MDKMRILKESRPEIIVGGKYRVIRKIGSGSFGDIYLGMSIQSGEEVAIKMESAHARHPQLLYEAKLYRILSGGVGFPRIRHHGKEKNFNTLVMDLLGPSLEDLFNFCTRHFTIKTVLMLVDQMIGRLEYIHLKCFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKFRDPHTRHHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGVLPWQGMKANTKQQKYEKISEKKMSTPIEVLCKGSPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRILFRTLNHQYDYIYDWTMLKQKTHQGQPNPAILLEQLDKDKEKQNGKPLIAD

Target ORF information:

RefSeq Version	NM_167332.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase ialpha, transcript variant G (CkIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167332.3

ORF Insert Sequence:

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIalpha ( NM_078585.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511140.1 Drosophila melanogaster casein kinase ialpha, transcript variant B (CkIalpha), mRNA.

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CloneID	OFa01421
Clone ID Related Accession (Same CDS sequence)	NM_001169266.3 , NM_001298235.1 , NM_167332.3 , NM_078585.6 , NM_167331.4 , NM_001298234.1
Accession Version	NM_078585.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase ialpha, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078585.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511140.1
CDS	437..1450
Translation	MDKMRILKESRPEIIVGGKYRVIRKIGSGSFGDIYLGMSIQSGEEVAIKMESAHARHPQLLYEAKLYRILSGGVGFPRIRHHGKEKNFNTLVMDLLGPSLEDLFNFCTRHFTIKTVLMLVDQMIGRLEYIHLKCFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKFRDPHTRHHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGVLPWQGMKANTKQQKYEKISEKKMSTPIEVLCKGSPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRILFRTLNHQYDYIYDWTMLKQKTHQGQPNPAILLEQLDKDKEKQNGKPLIAD

Target ORF information:

RefSeq Version	NM_078585.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase ialpha, transcript variant B (CkIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078585.6

ORF Insert Sequence:

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIalpha ( NM_167331.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727631.1 Drosophila melanogaster casein kinase ialpha, transcript variant A (CkIalpha), mRNA.

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CloneID	OFa01421
Clone ID Related Accession (Same CDS sequence)	NM_001169266.3 , NM_001298235.1 , NM_167332.3 , NM_078585.6 , NM_167331.4 , NM_001298234.1
Accession Version	NM_167331.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase ialpha, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167331.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727631.1
CDS	338..1351
Translation	MDKMRILKESRPEIIVGGKYRVIRKIGSGSFGDIYLGMSIQSGEEVAIKMESAHARHPQLLYEAKLYRILSGGVGFPRIRHHGKEKNFNTLVMDLLGPSLEDLFNFCTRHFTIKTVLMLVDQMIGRLEYIHLKCFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKFRDPHTRHHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGVLPWQGMKANTKQQKYEKISEKKMSTPIEVLCKGSPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRILFRTLNHQYDYIYDWTMLKQKTHQGQPNPAILLEQLDKDKEKQNGKPLIAD

Target ORF information:

RefSeq Version	NM_167331.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase ialpha, transcript variant A (CkIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167331.4

ORF Insert Sequence:

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CkIalpha ( NM_001298234.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285163.1 Drosophila melanogaster casein kinase ialpha, transcript variant E (CkIalpha), mRNA.

Price & Availability↑

CloneID	OFa01421
Clone ID Related Accession (Same CDS sequence)	NM_001169266.3 , NM_001298235.1 , NM_167332.3 , NM_078585.6 , NM_167331.4 , NM_001298234.1
Accession Version	NM_001298234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	casein kinase ialpha, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285163.1
CDS	611..1624
Translation	MDKMRILKESRPEIIVGGKYRVIRKIGSGSFGDIYLGMSIQSGEEVAIKMESAHARHPQLLYEAKLYRILSGGVGFPRIRHHGKEKNFNTLVMDLLGPSLEDLFNFCTRHFTIKTVLMLVDQMIGRLEYIHLKCFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKFRDPHTRHHIVYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVMMYFNRGVLPWQGMKANTKQQKYEKISEKKMSTPIEVLCKGSPAEFSMYLNYCRSLRFEEQPDYMYLRQLFRILFRTLNHQYDYIYDWTMLKQKTHQGQPNPAILLEQLDKDKEKQNGKPLIAD

Target ORF information:

RefSeq Version	NM_001298234.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster casein kinase ialpha, transcript variant E (CkIalpha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298234.1

ORF Insert Sequence:

ATGGACAAGA TGCGGATATT GAAGGAAAGT CGCCCCGAGA TAATCGTCGG TGGCAAATAT 
CGGGTGATCA GGAAGATTGG AAGCGGATCG TTTGGCGACA TTTACCTGGG CATGAGCATC 
CAGAGCGGCG AAGAAGTGGC CATCAAGATG GAGAGCGCCC ACGCCCGCCA TCCGCAGCTG 
TTGTACGAGG CCAAGCTGTA CCGCATTCTG AGCGGCGGCG TTGGATTCCC TCGTATACGT 
CACCATGGCA AGGAAAAGAA CTTCAACACC CTGGTCATGG ACCTGCTGGG ACCCTCGCTG 
GAGGATCTGT TCAATTTCTG TACGCGCCAT TTCACAATCA AAACGGTTCT GATGCTCGTC 
GACCAGATGA TCGGACGCTT GGAGTACATC CATCTCAAGT GCTTCATCCA TCGCGACATC 
AAGCCGGATA ACTTCCTAAT GGGCATTGGT CGGCACTGCA ATAAGCTGTT CCTGATCGAT 
TTCGGTCTGG CCAAGAAGTT CCGCGATCCG CACACGCGCC ATCACATCGT TTACCGCGAG 
GACAAGAACC TCACCGGCAC TGCCCGCTAT GCCTCGATCA ATGCCCATCT GGGCATCGAG 
CAGTCGCGGC GTGACGACAT GGAATCGCTT GGATACGTGA TGATGTACTT CAATCGCGGC 
GTACTGCCAT GGCAAGGCAT GAAGGCCAAC ACCAAGCAGC AGAAATACGA GAAGATCTCC 
GAAAAGAAGA TGTCCACGCC CATCGAGGTC CTCTGCAAGG GCTCGCCGGC CGAGTTCTCC 
ATGTATCTGA ACTATTGTCG TAGCCTGCGC TTCGAGGAGC AGCCAGATTA CATGTACCTA 
CGTCAATTGT TCCGCATACT GTTCAGAACG CTGAACCATC AGTATGACTA CATCTACGAC 
TGGACAATGC TGAAGCAGAA GACCCATCAG GGTCAACCCA ATCCAGCTAT ACTCTTGGAG 
CAATTGGACA AGGACAAGGA GAAGCAGAAC GGCAAGCCCC TGATCGCGGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CkIalpha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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