Sec16 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sec16
Entrez Gene ID	32209
Full Name	Secretory 16
Synonyms	BcDNA:RE70141,CG18079,CG2551,CG32654,Dmel\CG32654,dSec16,dsec16,sec16,sec26
General protein information	Preferred Names Secretory 16 Names CG32654 gene product from transcript CG32654-RJ CG32654-PD CG32654-PE CG32654-PF CG32654-PH CG32654-PI CG32654-PJ Sec16 ortholog Sec16-PD Sec16-PE Sec16-PF Sec16-PH Sec16-PI Sec16-PJ secretory 16
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11B2-11B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103493.4	NP_001096963.1	secretory 16, isoform E
NM_001298228.1	NP_001285157.1	secretory 16, isoform H
NM_001298229.1	NP_001285158.1	secretory 16, isoform I
NM_001298230.1	NP_001285159.1	secretory 16, isoform J
NM_132584.3	NP_572812.3	secretory 16, isoform F
NM_001103494.2	NP_001096964.1	secretory 16, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In response to amino acid starvation, ERK7-mediated post-translational modification elicits the cytoplasmic dispersion of Sec16 and the consequent disassembly of the ER exit sites, which in turn results in protein secretion inhibition.
Title: ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association.

dSec16 binds ER cups via its arginine-rich domain, interacts with Sar1-GTP that is generated on ER membrane by Sec12 and concentrates it in the ER cups where it initiates the formation of COPII vesicles.
Title: Drosophila Sec16 mediates the biogenesis of tER sites upstream of Sar1 through an arginine-rich motif.

The following Sec16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sec16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01409	NM_001103494.2 Latest version!	Drosophila melanogaster secretory 16, transcript variant D (Sec16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa01410	NM_132584.3 Latest version!	Drosophila melanogaster secretory 16, transcript variant F (Sec16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa01411	NM_001103493.4 Latest version!	Drosophila melanogaster secretory 16, transcript variant E (Sec16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa21444	NM_001298230.1 Latest version!	Drosophila melanogaster secretory 16, transcript variant J (Sec16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa21445	NM_001298228.1 Latest version!	Drosophila melanogaster secretory 16, transcript variant H (Sec16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa21446	NM_001298229.1 Latest version!	Drosophila melanogaster secretory 16, transcript variant I (Sec16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sec16 ( NM_001103494.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096964.1 Drosophila melanogaster secretory 16, transcript variant D (Sec16), mRNA.

Price & Availability↑

CloneID	OFa01409
Clone ID Related Accession (Same CDS sequence)	NM_001103494.2
Accession Version	NM_001103494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 16, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103494.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGA GCTGCTGCCG ATGCGGCACC TTCGACTTAT 
GGAGGAGTGG CTGGAACCGC TGGACCGGTG ACCAACACTG CGGTGGCTCG TCCGCCGCGC 
AGACGCACTC CCTTGCAGTT CAGCCGACCG CATTTGGTGG CCTCGTATGC GATGAGTCTG 
CTGCTGAAGG TGAAGCCCAA ATACGCGGGA CGTGGACGAC TGCGCAACGA TGTGGAGGTG 
GCACCACCAC GCATACGGGA CGGTACGAGC AGCCTGCTGC GCATGTATCC TGGTCCCTTG 
CAGGGACGCA AGTTGCACAA GGACAAGATC ATCAGTTTCT GCAAGGAGCA GATCCGACTG 
GGGCCCACAA AGGGTTGCAC CGCACTGTAT GCCACGCAGA AGAAGCCACA GGGCAGCGTG 
ACGAAATACC GTGCCTCCCA TGCGCTCATG TGGCACCTGC TAATACTTTT GCTGCGCCAA 
AATGGGTACA TTGCGGACAC GGATGTGGGC GATCTGCTGC TGGAGAACCA GCAAGAGTAT 
CCCTATGATC CCAGCGAGTT CGAAGCAGAG AACGAGCCAG ATGCGGATGC CGAACAGGAT 
GCCGCTGCAC CGGCGGACAA GACAGTCGAT TCGGATTTAG ATAGCGAATC GGCAGCAGGA 
GTTACACCTG CGGAACCACT TGCAGCAGGC GCGGCAACTA GCTCAATCAA TGGAGCTGAT 
GCTGCAAACG CAGCAACGCC TCTGTCGGAA CAGGCGGCAA CGGACAAGTT CCGCAGCTAT 
GTGCTGCGCG GAAATGTCGA GGAGGCTCTC CAGTGGGCAA CCGACAACGG CCTGTGGACG 
CATGCATTCT TCTTGGCCCT CTACGAGGAT CGCTATGCTC TCACTGACGT AGCCCAGAAG 
TTCCTCAATC GAGCGATCAA GGCCAACGAT CCATTGCAGA CACTATACCA AATGAAGAGC 
TGCCACACAC CGGCGTGCGT CAGCCAGCTG AGGGATGAAC AGTGGGGTGA CTGGCGATCG 
CATCTCTCCA TCCTGGTGAC GAACAAGAGT CGCCAGCCGG AGTATGATCG CAGTTCGGTA 
GTGGCCCTTG GCGATACGCT TTTCCAGCGC GGCGACATTT ATGCGGCACA CTTTTGCTAT 
CTGGTGGCTC AGGAGGAGTT CGGACGATAC GATAGCTCTG CTACAGAGCT AACTACTCTG 
ACCGCCAATG TGCCCAGGCT TATTCTACTT GGCTCCTCGC ACTACAAGCA CTTCAACGAA 
TTTGCCAGCA ACGAGGCGAT CATCATGACC GAGATCTATG AGTATGCTCG ATCGCTGTTC 
GATCCGAAGT TTAGCATTGC CCACTTCCAA CACTACAAGT TCCTGCTAGC CACAAGGATC 
CTAGATTATG GTCAGCATTT CCGCTGCACC AATTATTTGG AACAAATCGC CAGGCACATC 
GAACTGAAGC CGGAAAGCTA CGACAGTAAT TTCATTCAGC GGGTTTGCGG TTTGGCCGAA 
CGCCTGCGCT ACCATGATCC CATTCTCATT AATCGTGTCT CATTTGCGAG TCCTCCGAAT 
GCCACTAGCA AGGATAGTGC TGCTGCTGAA GAGAAGGCCT GGTTGCGTCA GCTTAGATCT 
CTGGCCTATG TGCAGCCCCA ACAAGAGCAG CTGCAACAAC TGCAGCAGAA TCAGCAAGTC 
CAGCAGGAGC AAAACGACAT CGATCAGCAG TTTGCAGAGG TGAACAAGCA ATTCAGGGAG 
CTAAACATGC AATACGAGAG CAACAGCAAC ATGGAGAATA CGCTGCAGCC GCAGTTGCCA 
CCGGTGGATC AACAGCCGCC GCAACAGCAG CAACAAGTTT TATCGGAATC GTACCAACAG 
CCAACACAAC AATATTATGA GCCACCACCT CAAGCGCCAA CCGAATCCGA TCCCTATGGA 
CAGGGCCAAC CGTCCTATTA TGATCCCAAT GCGGGGCAGC TTCATTACGA TCCAAATGCT 
GCACAGCATC AATACGACCA GCAGCAACCT CAACCACAGC CGACTCCCAG CTATGGCCAA 
ATAGAGCCAG GAACTGAGGC CTACCAACCT GGACAAGCAA CAGCAGATCC AGCTGCGGTT 
CCCTCTGGCT ACGGATACGA TTACTGGTCG GGCACACAGC AGCCACCTTA TGGTGACGAG 
CCCAATGAGA ATCAAGCTGC ACTAAAGGAT GAAGAGGAGG AGGATGAGGA GCAGGATGAA 
CAGCAGATAT TGCAGTACGC TAAACAGCAG ATTTTGCAGC AAACCAAGCA GCAGCAGCTG 
CAGCAGGAGC AACATACTCG CAGCAATGGC GCCGCCAGCA TCAACAATCG CTTCAAATCG 
AACGCTCTGA AGCAGGAACG CAGCAGCGGC TCATTGCGGA AAAGGCAAGA GGCATCACGA 
ACAGCAACAA TAGCAGCGGC AGCAGCAGCT ATTGCTCAGA TAACACCGGC AACAGTTGCA 
GCGGCAGCGA CGCCACCAGC AGCATCGGCA AAGGCATTCA ATTTGAATGA TGTAAGTAGC 
TTTTCCAGCA CAAATAAACG ACATCAAGCT ACCAGCAGCA GCAACAATCG CAGCAGCAGC 
AACCAAGCAG CAGCAGCAGC AGCAGCAACA AAAGCTGCAG CAATAGCAAC AGTACCTGCT 
GCCCCTAATC AATTGCTAAG TTGTAGAAAG TCTAACAGCC AGAATAGTCC ATACTTAGCC 
ACGCCAAAAA CGCTTACCAC GCCCACACGT TTTATTGCCG CTAAAGCAGC CACTGTTACA 
GCCACGCCCA CAGCGCCAGC CACTTCGACG ACAACGTCGA CAACCATAGG TGCTAGAGGA 
TCATCTACCA AACGCGTTTA CTTTAATCTT CCGTCGACCA TTGCTGAATC GGAGCAAGAG 
TTCGAACATG AGCTGGATTA TGATGAGGAG ATGGAGGAGG AGAACAGTAT GGCTCTGGCG 
CAAGCTAAAC CAAACGTTCA TCTAGCGCGA AAGCCACATA TTGTCTTTAA TGGAACGTAT 
CCCATTGATA TGCCACTGAC CCAGGGGCAG AGTCAACTCA AAAGCAACTC GTATGATCCG 
GAGGCCAATG CAGATCGTGG GGATCTACAG CGCTATAGGA ACTACATGCT CCACGATTAT 
GTTATAGATG AGGATGCCGA GGGCGAGGAG CAGGCCGAAG AAGAGGGCGA GGAGGATGAG 
TATGGAGATG GTGATGAGGA TTTATTCCCC TATGCCGACG ACGATGAGAG CTTCGATGAT 
GGCGATTACT ATACGCATCA ACTGCTCAAT AAGTATGGGA TTATACCAAG CGGTGAGAAA 
CAGAGAGTCG AGAGAACACC GGCATCCGTG GAGGCAGCTA CCTCCGCGGC CCAGCCACAG 
CACGTGTGGT CCATGAAGGA GCTGATAGCC AATCCAGCCA TACCACTTAA CTACAAGAAA 
ATGTGCTCCG AAGTGGAGCA GAGCCTCAGT CGATTCGAGA ATTATTTGGA CAGCAAAAAG 
GAGCAACAGG TGCCACCGGG AGCCGCAGTG CGCACCTTCG ACATCGATGC ACCCACTCGA 
AGAGGCCACC GAAGCAGCAC ATCCACAAAT GCTGGCTTGG ATCTTAGATC TAAGAAGAAA 
TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096964.1
CDS	196..6861
Translation	MLHNNAPWLPPHQQQQQQAPQQHPTPQQQHAMPQQQQTLPAQQQQQQVYASQMFQQHQPNYWPEDQHQQQQQPQSLNYNNYFPGQQQQHPMQQQQLPPQQQLPPQQQQPTQQQHTIQQQLYYPTQQQPQVPAPAEPALDSFDNNNSGGGGGGGRSDGWGDWGDWNDNSNNNNSNGTDGLLEPTGQLLEDSFNVQSSQGSWQAFATSNNVNNNGELPPPANPQSTSLQQQPLNQQSIPLLHQQPQSPPELGQEPELDAIVPPQAFQNQPPTAAPPPTSLTSFSAQMTAVGSPIYAVGASSAHSAGVGASPAPPTGVVAPPAHLAGIGAPPAHLAGVGASPAPSALVGASPAPIIGVGAPPVGAPPVGAPPADATTIAPPAALPPSIVPSSGSNPFKRSTGLNKRVNIMADPAAGAPSPPAPAAAIAPVAPVAPIPPPADQLFGMPAEAHGEGFNLIAAPPVEASLGTPLSAPIPAPIPVPNASLYASPAVPQAFAHLEPDNQEVLSAPNDERAQYLQTSHLSEQLGEGEADQDAGLLPPPGLSRLVLGQPELDSQQQRQVTGATEQPPLNVAQAAALHMQERRADGEDTSDGEQQVRNIQTPPRRVVTGVETNAPSLREQREVVLDGENLEDREAIPPPTLAELPTTSVHHNILPDEAEQLHHNNPPQAMTPLNAQQPHQQQSTLQQQHQQHSTTQQEKKRAAVGRRTTASLDLESDESDEFLQSERERDRERERRDLMEERHSRGRSHSHYPYEGETEDSVRGAAHHETKSLRETHHKRNHDSARSRRHQDPKVERERERDRERDRTWRRRSNKYHSGGEDPDRSYDHSRRYNNSNYEGESDNPEYNHMGDAELDGSGGRSSKTSRHRRSAAEEDFDDYERERNRSRRSTKPQSSAEKSRSSGGRRNYENSGRSARADDGRRRYQDQRNPGAQYPVSATGYVPYGMYEQMSRNPQVYADMYAKFYGQMINSMNAAVSAAASKGGVPAGAGVIPGLMPSAVPVSAAQLVAATSGGSVSGSSEAAMLRERERAAADAAPSTYGGVAGTAGPVTNTAVARPPRRRTPLQFSRPHLVASYAMSLLLKVKPKYAGRGRLRNDVEVAPPRIRDGTSSLLRMYPGPLQGRKLHKDKIISFCKEQIRLGPTKGCTALYATQKKPQGSVTKYRASHALMWHLLILLLRQNGYIADTDVGDLLLENQQEYPYDPSEFEAENEPDADAEQDAAAPADKTVDSDLDSESAAGVTPAEPLAAGAATSSINGADAANAATPLSEQAATDKFRSYVLRGNVEEALQWATDNGLWTHAFFLALYEDRYALTDVAQKFLNRAIKANDPLQTLYQMKSCHTPACVSQLRDEQWGDWRSHLSILVTNKSRQPEYDRSSVVALGDTLFQRGDIYAAHFCYLVAQEEFGRYDSSATELTTLTANVPRLILLGSSHYKHFNEFASNEAIIMTEIYEYARSLFDPKFSIAHFQHYKFLLATRILDYGQHFRCTNYLEQIARHIELKPESYDSNFIQRVCGLAERLRYHDPILINRVSFASPPNATSKDSAAAEEKAWLRQLRSLAYVQPQQEQLQQLQQNQQVQQEQNDIDQQFAEVNKQFRELNMQYESNSNMENTLQPQLPPVDQQPPQQQQQVLSESYQQPTQQYYEPPPQAPTESDPYGQGQPSYYDPNAGQLHYDPNAAQHQYDQQQPQPQPTPSYGQIEPGTEAYQPGQATADPAAVPSGYGYDYWSGTQQPPYGDEPNENQAALKDEEEEDEEQDEQQILQYAKQQILQQTKQQQLQQEQHTRSNGAASINNRFKSNALKQERSSGSLRKRQEASRTATIAAAAAAIAQITPATVAAAATPPAASAKAFNLNDVSSFSSTNKRHQATSSSNNRSSSNQAAAAAAATKAAAIATVPAAPNQLLSCRKSNSQNSPYLATPKTLTTPTRFIAAKAATVTATPTAPATSTTTSTTIGARGSSTKRVYFNLPSTIAESEQEFEHELDYDEEMEEENSMALAQAKPNVHLARKPHIVFNGTYPIDMPLTQGQSQLKSNSYDPEANADRGDLQRYRNYMLHDYVIDEDAEGEEQAEEEGEEDEYGDGDEDLFPYADDDESFDDGDYYTHQLLNKYGIIPSGEKQRVERTPASVEAATSAAQPQHVWSMKELIANPAIPLNYKKMCSEVEQSLSRFENYLDSKKEQQVPPGAAVRTFDIDAPTRRGHRSSTSTNAGLDLRSKKKL

Target ORF information:

RefSeq Version	NM_001103494.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 16, transcript variant D (Sec16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103494.2

ORF Insert Sequence:

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGA GCTGCTGCCG ATGCGGCACC TTCGACTTAT 
GGAGGAGTGG CTGGAACCGC TGGACCGGTG ACCAACACTG CGGTGGCTCG TCCGCCGCGC 
AGACGCACTC CCTTGCAGTT CAGCCGACCG CATTTGGTGG CCTCGTATGC GATGAGTCTG 
CTGCTGAAGG TGAAGCCCAA ATACGCGGGA CGTGGACGAC TGCGCAACGA TGTGGAGGTG 
GCACCACCAC GCATACGGGA CGGTACGAGC AGCCTGCTGC GCATGTATCC TGGTCCCTTG 
CAGGGACGCA AGTTGCACAA GGACAAGATC ATCAGTTTCT GCAAGGAGCA GATCCGACTG 
GGGCCCACAA AGGGTTGCAC CGCACTGTAT GCCACGCAGA AGAAGCCACA GGGCAGCGTG 
ACGAAATACC GTGCCTCCCA TGCGCTCATG TGGCACCTGC TAATACTTTT GCTGCGCCAA 
AATGGGTACA TTGCGGACAC GGATGTGGGC GATCTGCTGC TGGAGAACCA GCAAGAGTAT 
CCCTATGATC CCAGCGAGTT CGAAGCAGAG AACGAGCCAG ATGCGGATGC CGAACAGGAT 
GCCGCTGCAC CGGCGGACAA GACAGTCGAT TCGGATTTAG ATAGCGAATC GGCAGCAGGA 
GTTACACCTG CGGAACCACT TGCAGCAGGC GCGGCAACTA GCTCAATCAA TGGAGCTGAT 
GCTGCAAACG CAGCAACGCC TCTGTCGGAA CAGGCGGCAA CGGACAAGTT CCGCAGCTAT 
GTGCTGCGCG GAAATGTCGA GGAGGCTCTC CAGTGGGCAA CCGACAACGG CCTGTGGACG 
CATGCATTCT TCTTGGCCCT CTACGAGGAT CGCTATGCTC TCACTGACGT AGCCCAGAAG 
TTCCTCAATC GAGCGATCAA GGCCAACGAT CCATTGCAGA CACTATACCA AATGAAGAGC 
TGCCACACAC CGGCGTGCGT CAGCCAGCTG AGGGATGAAC AGTGGGGTGA CTGGCGATCG 
CATCTCTCCA TCCTGGTGAC GAACAAGAGT CGCCAGCCGG AGTATGATCG CAGTTCGGTA 
GTGGCCCTTG GCGATACGCT TTTCCAGCGC GGCGACATTT ATGCGGCACA CTTTTGCTAT 
CTGGTGGCTC AGGAGGAGTT CGGACGATAC GATAGCTCTG CTACAGAGCT AACTACTCTG 
ACCGCCAATG TGCCCAGGCT TATTCTACTT GGCTCCTCGC ACTACAAGCA CTTCAACGAA 
TTTGCCAGCA ACGAGGCGAT CATCATGACC GAGATCTATG AGTATGCTCG ATCGCTGTTC 
GATCCGAAGT TTAGCATTGC CCACTTCCAA CACTACAAGT TCCTGCTAGC CACAAGGATC 
CTAGATTATG GTCAGCATTT CCGCTGCACC AATTATTTGG AACAAATCGC CAGGCACATC 
GAACTGAAGC CGGAAAGCTA CGACAGTAAT TTCATTCAGC GGGTTTGCGG TTTGGCCGAA 
CGCCTGCGCT ACCATGATCC CATTCTCATT AATCGTGTCT CATTTGCGAG TCCTCCGAAT 
GCCACTAGCA AGGATAGTGC TGCTGCTGAA GAGAAGGCCT GGTTGCGTCA GCTTAGATCT 
CTGGCCTATG TGCAGCCCCA ACAAGAGCAG CTGCAACAAC TGCAGCAGAA TCAGCAAGTC 
CAGCAGGAGC AAAACGACAT CGATCAGCAG TTTGCAGAGG TGAACAAGCA ATTCAGGGAG 
CTAAACATGC AATACGAGAG CAACAGCAAC ATGGAGAATA CGCTGCAGCC GCAGTTGCCA 
CCGGTGGATC AACAGCCGCC GCAACAGCAG CAACAAGTTT TATCGGAATC GTACCAACAG 
CCAACACAAC AATATTATGA GCCACCACCT CAAGCGCCAA CCGAATCCGA TCCCTATGGA 
CAGGGCCAAC CGTCCTATTA TGATCCCAAT GCGGGGCAGC TTCATTACGA TCCAAATGCT 
GCACAGCATC AATACGACCA GCAGCAACCT CAACCACAGC CGACTCCCAG CTATGGCCAA 
ATAGAGCCAG GAACTGAGGC CTACCAACCT GGACAAGCAA CAGCAGATCC AGCTGCGGTT 
CCCTCTGGCT ACGGATACGA TTACTGGTCG GGCACACAGC AGCCACCTTA TGGTGACGAG 
CCCAATGAGA ATCAAGCTGC ACTAAAGGAT GAAGAGGAGG AGGATGAGGA GCAGGATGAA 
CAGCAGATAT TGCAGTACGC TAAACAGCAG ATTTTGCAGC AAACCAAGCA GCAGCAGCTG 
CAGCAGGAGC AACATACTCG CAGCAATGGC GCCGCCAGCA TCAACAATCG CTTCAAATCG 
AACGCTCTGA AGCAGGAACG CAGCAGCGGC TCATTGCGGA AAAGGCAAGA GGCATCACGA 
ACAGCAACAA TAGCAGCGGC AGCAGCAGCT ATTGCTCAGA TAACACCGGC AACAGTTGCA 
GCGGCAGCGA CGCCACCAGC AGCATCGGCA AAGGCATTCA ATTTGAATGA TGTAAGTAGC 
TTTTCCAGCA CAAATAAACG ACATCAAGCT ACCAGCAGCA GCAACAATCG CAGCAGCAGC 
AACCAAGCAG CAGCAGCAGC AGCAGCAACA AAAGCTGCAG CAATAGCAAC AGTACCTGCT 
GCCCCTAATC AATTGCTAAG TTGTAGAAAG TCTAACAGCC AGAATAGTCC ATACTTAGCC 
ACGCCAAAAA CGCTTACCAC GCCCACACGT TTTATTGCCG CTAAAGCAGC CACTGTTACA 
GCCACGCCCA CAGCGCCAGC CACTTCGACG ACAACGTCGA CAACCATAGG TGCTAGAGGA 
TCATCTACCA AACGCGTTTA CTTTAATCTT CCGTCGACCA TTGCTGAATC GGAGCAAGAG 
TTCGAACATG AGCTGGATTA TGATGAGGAG ATGGAGGAGG AGAACAGTAT GGCTCTGGCG 
CAAGCTAAAC CAAACGTTCA TCTAGCGCGA AAGCCACATA TTGTCTTTAA TGGAACGTAT 
CCCATTGATA TGCCACTGAC CCAGGGGCAG AGTCAACTCA AAAGCAACTC GTATGATCCG 
GAGGCCAATG CAGATCGTGG GGATCTACAG CGCTATAGGA ACTACATGCT CCACGATTAT 
GTTATAGATG AGGATGCCGA GGGCGAGGAG CAGGCCGAAG AAGAGGGCGA GGAGGATGAG 
TATGGAGATG GTGATGAGGA TTTATTCCCC TATGCCGACG ACGATGAGAG CTTCGATGAT 
GGCGATTACT ATACGCATCA ACTGCTCAAT AAGTATGGGA TTATACCAAG CGGTGAGAAA 
CAGAGAGTCG AGAGAACACC GGCATCCGTG GAGGCAGCTA CCTCCGCGGC CCAGCCACAG 
CACGTGTGGT CCATGAAGGA GCTGATAGCC AATCCAGCCA TACCACTTAA CTACAAGAAA 
ATGTGCTCCG AAGTGGAGCA GAGCCTCAGT CGATTCGAGA ATTATTTGGA CAGCAAAAAG 
GAGCAACAGG TGCCACCGGG AGCCGCAGTG CGCACCTTCG ACATCGATGC ACCCACTCGA 
AGAGGCCACC GAAGCAGCAC ATCCACAAAT GCTGGCTTGG ATCTTAGATC TAAGAAGAAA 
TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec16 ( NM_132584.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572812.3 Drosophila melanogaster secretory 16, transcript variant F (Sec16), mRNA.

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CloneID	OFa01410
Clone ID Related Accession (Same CDS sequence)	NM_132584.3
Accession Version	NM_132584.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 16, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132584.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGG TATACACACG CATACATAAC CCAAGCCAAT 
GAATTCCATC GTCACCAATA CAAAGAGCTG ATCTACCAGC AACAACAACA ACAACAGCAG 
CAGCAACAAC AACAACAGCA GAGGGAGGAT CACCTGAACT CCAGCTTTAG CGTTGCAGAG 
GATAATGCCA GTTTTTACGG ATCGCACGGA GGAGGTTCCA TCTATAACCA ATACCAATCG 
TATCCACTAT CCAGTGCCCG ATCGTTGAGT AACTTGAACG GCGATGGACG CAATTCCCGA 
TGCGGACCGT ATTACGCTGG TTCCGAATGC GGTCTCGATA TCAGAGCTGC TGCCGATGCG 
GCACCTTCGA CTTATGGAGG AGTGGCTGGA ACCGCTGGAC CGGTGACCAA CACTGCGGTG 
GCTCGTCCGC CGCGCAGACG CACTCCCTTG CAGTTCAGCC GACCGCATTT GGTGGCCTCG 
TATGCGATGA GTCTGCTGCT GAAGGTGAAG CCCAAATACG CGGGACGTGG ACGACTGCGC 
AACGATGTGG AGGTGGCACC ACCACGCATA CGGGACGGTA CGAGCAGCCT GCTGCGCATG 
TATCCTGGTC CCTTGCAGGG ACGCAAGTTG CACAAGGACA AGATCATCAG TTTCTGCAAG 
GAGCAGATCC GACTGGGGCC CACAAAGGGT TGCACCGCAC TGTATGCCAC GCAGAAGAAG 
CCACAGGGCA GCGTGACGAA ATACCGTGCC TCCCATGCGC TCATGTGGCA CCTGCTAATA 
CTTTTGCTGC GCCAAAATGG GTACATTGCG GACACGGATG TGGGCGATCT GCTGCTGGAG 
AACCAGCAAG AGTATCCCTA TGATCCCAGC GAGTTCGAAG CAGAGAACGA GCCAGATGCG 
GATGCCGAAC AGGATGCCGC TGCACCGGCG GACAAGACAG TCGATTCGGA TTTAGATAGC 
GAATCGGCAG CAGGAGTTAC ACCTGCGGAA CCACTTGCAG CAGGCGCGGC AACTAGCTCA 
ATCAATGGAG CTGATGCTGC AAACGCAGCA ACGCCTCTGT CGGAACAGGC GGCAACGGAC 
AAGTTCCGCA GCTATGTGCT GCGCGGAAAT GTCGAGGAGG CTCTCCAGTG GGCAACCGAC 
AACGGCCTGT GGACGCATGC ATTCTTCTTG GCCCTCTACG AGGATCGCTA TGCTCTCACT 
GACGTAGCCC AGAAGTTCCT CAATCGAGCG ATCAAGGCCA ACGATCCATT GCAGACACTA 
TACCAAATGA AGAGCTGCCA CACACCGGCG TGCGTCAGCC AGCTGAGGGA TGAACAGTGG 
GGTGACTGGC GATCGCATCT CTCCATCCTG GTGACGAACA AGAGTCGCCA GCCGGAGTAT 
GATCGCAGTT CGGTAGTGGC CCTTGGCGAT ACGCTTTTCC AGCGCGGCGA CATTTATGCG 
GCACACTTTT GCTATCTGGT GGCTCAGGAG GAGTTCGGAC GATACGATAG CTCTGCTACA 
GAGCTAACTA CTCTGACCGC CAATGTGCCC AGGCTTATTC TACTTGGCTC CTCGCACTAC 
AAGCACTTCA ACGAATTTGC CAGCAACGAG GCGATCATCA TGACCGAGAT CTATGAGTAT 
GCTCGATCGC TGTTCGATCC GAAGTTTAGC ATTGCCCACT TCCAACACTA CAAGTTCCTG 
CTAGCCACAA GGATCCTAGA TTATGGTCAG CATTTCCGCT GCACCAATTA TTTGGAACAA 
ATCGCCAGGC ACATCGAACT GAAGCCGGAA AGCTACGACA GTAATTTCAT TCAGCGGGTT 
TGCGGTTTGG CCGAACGCCT GCGCTACCAT GATCCCATTC TCATTAATCG TGTCTCATTT 
GCGAGTCCTC CGAATGCCAC TAGCAAGGAT AGTGCTGCTG CTGAAGAGAA GGCCTGGTTG 
CGTCAGCTTA GATCTCTGGC CTATGTGCAG CCCCAACAAG AGCAGCTGCA ACAACTGCAG 
CAGAATCAGC AAGTCCAGCA GGAGCAAAAC GACATCGATC AGCAGTTTGC AGAGGTGAAC 
AAGCAATTCA GGGAGCTAAA CATGCAATAC GAGAGCAACA GCAACATGGA GAATACGCTG 
CAGCCGCAGT TGCCACCGGT GGATCAACAG CCGCCGCAAC AGCAGCAACA AGTTTTATCG 
GAATCGTACC AACAGCCAAC ACAACAATAT TATGAGCCAC CACCTCAAGC GCCAACCGAA 
TCCGATCCCT ATGGACAGGG CCAACCGTCC TATTATGATC CCAATGCGGG GCAGCTTCAT 
TACGATCCAA ATGCTGCACA GCATCAATAC GACCAGCAGC AACCTCAACC ACAGCCGACT 
CCCAGCTATG GCCAAATAGA GCCAGGAACT GAGGCCTACC AACCTGGACA AGCAACAGCA 
GATCCAGCTG CGGTTCCCTC TGGCTACGGA TACGATTACT GGTCGGGCAC ACAGCAGCCA 
CCTTATGGTG ACGAGCCCAA TGAGAATCAA GCTGCACTAA AGGATGAAGA GGAGGAGGAT 
GAGGAGCAGG ATGAACAGCA GATATTGCAG TACGCTAAAC AGCAGATTTT GCAGCAAACC 
AAGCAGCAGC AGCTGCAGCA GGAGCAACAT ACTCGCAGCA ATGGCGCCGC CAGCATCAAC 
AATCGCTTCA AATCGAACGC TCTGAAGCAG GAACGCAGCA GCGGCTCATT GCGGAAAAGG 
CAAGAGGCAT CACGAACAGC AACAATAGCA GCGGCAGCAG CAGCTATTGC TCAGATAACA 
CCGGCAACAG TTGCAGCGGC AGCGACGCCA CCAGCAGCAT CGGCAAAGGC ATTCAATTTG 
AATGATCTGC AGCAACAGAC GCGCCCGGCG ATATCCATGC CCAAGTCAAA GAGCTACGGA 
GATGAGGACG ATGGTGGTTC CGGTGGTGTG GCTGATCCTG GTCAGAAAAC CAAGCCTGTA 
GGCTCCTCTG AGGCTGCAGG CAAACAGCAG GCGTCGGGCA AACAGGCCAA CTCCGGCAGC 
GATCTTGGTA TATATCATTT TGAACACAGC GCACCTGGGA ATAAAAATGC GGGCTGGTTT 
GGCGGACTCT GGAACAAGTT GTCGCTAAAG CCGAAGAACC AAATGATCCT GCCGGATGAC 
AAGAATCCGG CCATCGTCTG GGACAAGGAG CGCAAATGCT GGACAAACAC CGAAGGCAAC 
GGCGACGAGG CTGAAAGCTT TAAGCCGCCA CCCAAAATGA GTGATCTGGG CATGGCTCTG 
GGAGGACCAC CCGCTGCGCC AATCGGTAAT GCCGGATTGG GAATGGGTTT AATGCCTGTG 
GCTAACAATG CGCCGGGCAG CCATCAATCA GCACCGTTGA TGGATCAGCA AACGCCGTCT 
CAGCCGCAAA CGTATGGCAG CCCCATTGAT TACACGGCTG CTCCGGCACC CGAGCTGATT 
CCAACGGTAC CATCGCCGGC TCCACTTTCT GTCCCGTCAT CTGCACCGGC GGCATTAGCG 
GCGCCCAATC CCGCGGCCGT TCCCGGCGGA CCGCAGCCCA AGCTGCAGTC GAACATGTTC 
AAGATGCAAC GCAACCGCAC GCTAAAGAAC TCCTACGTGG ATGTGTTCAA TCCTTCGGGT 
GCGCCAATGT CGGCGGCACC GGAGAATGTG CTGGCCCCCA TGATGGCGCC GGCTGCGGTG 
CCCCAAGGCG GTTACTTTGT ACCCCAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572812.3
CDS	70..6822
Translation	MLHNNAPWLPPHQQQQQQAPQQHPTPQQQHAMPQQQQTLPAQQQQQQVYASQMFQQHQPNYWPEDQHQQQQQPQSLNYNNYFPGQQQQHPMQQQQLPPQQQLPPQQQQPTQQQHTIQQQLYYPTQQQPQVPAPAEPALDSFDNNNSGGGGGGGRSDGWGDWGDWNDNSNNNNSNGTDGLLEPTGQLLEDSFNVQSSQGSWQAFATSNNVNNNGELPPPANPQSTSLQQQPLNQQSIPLLHQQPQSPPELGQEPELDAIVPPQAFQNQPPTAAPPPTSLTSFSAQMTAVGSPIYAVGASSAHSAGVGASPAPPTGVVAPPAHLAGIGAPPAHLAGVGASPAPSALVGASPAPIIGVGAPPVGAPPVGAPPADATTIAPPAALPPSIVPSSGSNPFKRSTGLNKRVNIMADPAAGAPSPPAPAAAIAPVAPVAPIPPPADQLFGMPAEAHGEGFNLIAAPPVEASLGTPLSAPIPAPIPVPNASLYASPAVPQAFAHLEPDNQEVLSAPNDERAQYLQTSHLSEQLGEGEADQDAGLLPPPGLSRLVLGQPELDSQQQRQVTGATEQPPLNVAQAAALHMQERRADGEDTSDGEQQVRNIQTPPRRVVTGVETNAPSLREQREVVLDGENLEDREAIPPPTLAELPTTSVHHNILPDEAEQLHHNNPPQAMTPLNAQQPHQQQSTLQQQHQQHSTTQQEKKRAAVGRRTTASLDLESDESDEFLQSERERDRERERRDLMEERHSRGRSHSHYPYEGETEDSVRGAAHHETKSLRETHHKRNHDSARSRRHQDPKVERERERDRERDRTWRRRSNKYHSGGEDPDRSYDHSRRYNNSNYEGESDNPEYNHMGDAELDGSGGRSSKTSRHRRSAAEEDFDDYERERNRSRRSTKPQSSAEKSRSSGGRRNYENSGRSARADDGRRRYQDQRNPGAQYPVSATGYVPYGMYEQMSRNPQVYADMYAKFYGQMINSMNAAVSAAASKGGVPAGAGVIPGLMPSAVPVSAAQLVAATSGGSVSGSSEAAMLRERERYTHAYITQANEFHRHQYKELIYQQQQQQQQQQQQQQQREDHLNSSFSVAEDNASFYGSHGGGSIYNQYQSYPLSSARSLSNLNGDGRNSRCGPYYAGSECGLDIRAAADAAPSTYGGVAGTAGPVTNTAVARPPRRRTPLQFSRPHLVASYAMSLLLKVKPKYAGRGRLRNDVEVAPPRIRDGTSSLLRMYPGPLQGRKLHKDKIISFCKEQIRLGPTKGCTALYATQKKPQGSVTKYRASHALMWHLLILLLRQNGYIADTDVGDLLLENQQEYPYDPSEFEAENEPDADAEQDAAAPADKTVDSDLDSESAAGVTPAEPLAAGAATSSINGADAANAATPLSEQAATDKFRSYVLRGNVEEALQWATDNGLWTHAFFLALYEDRYALTDVAQKFLNRAIKANDPLQTLYQMKSCHTPACVSQLRDEQWGDWRSHLSILVTNKSRQPEYDRSSVVALGDTLFQRGDIYAAHFCYLVAQEEFGRYDSSATELTTLTANVPRLILLGSSHYKHFNEFASNEAIIMTEIYEYARSLFDPKFSIAHFQHYKFLLATRILDYGQHFRCTNYLEQIARHIELKPESYDSNFIQRVCGLAERLRYHDPILINRVSFASPPNATSKDSAAAEEKAWLRQLRSLAYVQPQQEQLQQLQQNQQVQQEQNDIDQQFAEVNKQFRELNMQYESNSNMENTLQPQLPPVDQQPPQQQQQVLSESYQQPTQQYYEPPPQAPTESDPYGQGQPSYYDPNAGQLHYDPNAAQHQYDQQQPQPQPTPSYGQIEPGTEAYQPGQATADPAAVPSGYGYDYWSGTQQPPYGDEPNENQAALKDEEEEDEEQDEQQILQYAKQQILQQTKQQQLQQEQHTRSNGAASINNRFKSNALKQERSSGSLRKRQEASRTATIAAAAAAIAQITPATVAAAATPPAASAKAFNLNDLQQQTRPAISMPKSKSYGDEDDGGSGGVADPGQKTKPVGSSEAAGKQQASGKQANSGSDLGIYHFEHSAPGNKNAGWFGGLWNKLSLKPKNQMILPDDKNPAIVWDKERKCWTNTEGNGDEAESFKPPPKMSDLGMALGGPPAAPIGNAGLGMGLMPVANNAPGSHQSAPLMDQQTPSQPQTYGSPIDYTAAPAPELIPTVPSPAPLSVPSSAPAALAAPNPAAVPGGPQPKLQSNMFKMQRNRTLKNSYVDVFNPSGAPMSAAPENVLAPMMAPAAVPQGGYFVPQQ

Target ORF information:

RefSeq Version	NM_132584.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 16, transcript variant F (Sec16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132584.3

ORF Insert Sequence:

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGG TATACACACG CATACATAAC CCAAGCCAAT 
GAATTCCATC GTCACCAATA CAAAGAGCTG ATCTACCAGC AACAACAACA ACAACAGCAG 
CAGCAACAAC AACAACAGCA GAGGGAGGAT CACCTGAACT CCAGCTTTAG CGTTGCAGAG 
GATAATGCCA GTTTTTACGG ATCGCACGGA GGAGGTTCCA TCTATAACCA ATACCAATCG 
TATCCACTAT CCAGTGCCCG ATCGTTGAGT AACTTGAACG GCGATGGACG CAATTCCCGA 
TGCGGACCGT ATTACGCTGG TTCCGAATGC GGTCTCGATA TCAGAGCTGC TGCCGATGCG 
GCACCTTCGA CTTATGGAGG AGTGGCTGGA ACCGCTGGAC CGGTGACCAA CACTGCGGTG 
GCTCGTCCGC CGCGCAGACG CACTCCCTTG CAGTTCAGCC GACCGCATTT GGTGGCCTCG 
TATGCGATGA GTCTGCTGCT GAAGGTGAAG CCCAAATACG CGGGACGTGG ACGACTGCGC 
AACGATGTGG AGGTGGCACC ACCACGCATA CGGGACGGTA CGAGCAGCCT GCTGCGCATG 
TATCCTGGTC CCTTGCAGGG ACGCAAGTTG CACAAGGACA AGATCATCAG TTTCTGCAAG 
GAGCAGATCC GACTGGGGCC CACAAAGGGT TGCACCGCAC TGTATGCCAC GCAGAAGAAG 
CCACAGGGCA GCGTGACGAA ATACCGTGCC TCCCATGCGC TCATGTGGCA CCTGCTAATA 
CTTTTGCTGC GCCAAAATGG GTACATTGCG GACACGGATG TGGGCGATCT GCTGCTGGAG 
AACCAGCAAG AGTATCCCTA TGATCCCAGC GAGTTCGAAG CAGAGAACGA GCCAGATGCG 
GATGCCGAAC AGGATGCCGC TGCACCGGCG GACAAGACAG TCGATTCGGA TTTAGATAGC 
GAATCGGCAG CAGGAGTTAC ACCTGCGGAA CCACTTGCAG CAGGCGCGGC AACTAGCTCA 
ATCAATGGAG CTGATGCTGC AAACGCAGCA ACGCCTCTGT CGGAACAGGC GGCAACGGAC 
AAGTTCCGCA GCTATGTGCT GCGCGGAAAT GTCGAGGAGG CTCTCCAGTG GGCAACCGAC 
AACGGCCTGT GGACGCATGC ATTCTTCTTG GCCCTCTACG AGGATCGCTA TGCTCTCACT 
GACGTAGCCC AGAAGTTCCT CAATCGAGCG ATCAAGGCCA ACGATCCATT GCAGACACTA 
TACCAAATGA AGAGCTGCCA CACACCGGCG TGCGTCAGCC AGCTGAGGGA TGAACAGTGG 
GGTGACTGGC GATCGCATCT CTCCATCCTG GTGACGAACA AGAGTCGCCA GCCGGAGTAT 
GATCGCAGTT CGGTAGTGGC CCTTGGCGAT ACGCTTTTCC AGCGCGGCGA CATTTATGCG 
GCACACTTTT GCTATCTGGT GGCTCAGGAG GAGTTCGGAC GATACGATAG CTCTGCTACA 
GAGCTAACTA CTCTGACCGC CAATGTGCCC AGGCTTATTC TACTTGGCTC CTCGCACTAC 
AAGCACTTCA ACGAATTTGC CAGCAACGAG GCGATCATCA TGACCGAGAT CTATGAGTAT 
GCTCGATCGC TGTTCGATCC GAAGTTTAGC ATTGCCCACT TCCAACACTA CAAGTTCCTG 
CTAGCCACAA GGATCCTAGA TTATGGTCAG CATTTCCGCT GCACCAATTA TTTGGAACAA 
ATCGCCAGGC ACATCGAACT GAAGCCGGAA AGCTACGACA GTAATTTCAT TCAGCGGGTT 
TGCGGTTTGG CCGAACGCCT GCGCTACCAT GATCCCATTC TCATTAATCG TGTCTCATTT 
GCGAGTCCTC CGAATGCCAC TAGCAAGGAT AGTGCTGCTG CTGAAGAGAA GGCCTGGTTG 
CGTCAGCTTA GATCTCTGGC CTATGTGCAG CCCCAACAAG AGCAGCTGCA ACAACTGCAG 
CAGAATCAGC AAGTCCAGCA GGAGCAAAAC GACATCGATC AGCAGTTTGC AGAGGTGAAC 
AAGCAATTCA GGGAGCTAAA CATGCAATAC GAGAGCAACA GCAACATGGA GAATACGCTG 
CAGCCGCAGT TGCCACCGGT GGATCAACAG CCGCCGCAAC AGCAGCAACA AGTTTTATCG 
GAATCGTACC AACAGCCAAC ACAACAATAT TATGAGCCAC CACCTCAAGC GCCAACCGAA 
TCCGATCCCT ATGGACAGGG CCAACCGTCC TATTATGATC CCAATGCGGG GCAGCTTCAT 
TACGATCCAA ATGCTGCACA GCATCAATAC GACCAGCAGC AACCTCAACC ACAGCCGACT 
CCCAGCTATG GCCAAATAGA GCCAGGAACT GAGGCCTACC AACCTGGACA AGCAACAGCA 
GATCCAGCTG CGGTTCCCTC TGGCTACGGA TACGATTACT GGTCGGGCAC ACAGCAGCCA 
CCTTATGGTG ACGAGCCCAA TGAGAATCAA GCTGCACTAA AGGATGAAGA GGAGGAGGAT 
GAGGAGCAGG ATGAACAGCA GATATTGCAG TACGCTAAAC AGCAGATTTT GCAGCAAACC 
AAGCAGCAGC AGCTGCAGCA GGAGCAACAT ACTCGCAGCA ATGGCGCCGC CAGCATCAAC 
AATCGCTTCA AATCGAACGC TCTGAAGCAG GAACGCAGCA GCGGCTCATT GCGGAAAAGG 
CAAGAGGCAT CACGAACAGC AACAATAGCA GCGGCAGCAG CAGCTATTGC TCAGATAACA 
CCGGCAACAG TTGCAGCGGC AGCGACGCCA CCAGCAGCAT CGGCAAAGGC ATTCAATTTG 
AATGATCTGC AGCAACAGAC GCGCCCGGCG ATATCCATGC CCAAGTCAAA GAGCTACGGA 
GATGAGGACG ATGGTGGTTC CGGTGGTGTG GCTGATCCTG GTCAGAAAAC CAAGCCTGTA 
GGCTCCTCTG AGGCTGCAGG CAAACAGCAG GCGTCGGGCA AACAGGCCAA CTCCGGCAGC 
GATCTTGGTA TATATCATTT TGAACACAGC GCACCTGGGA ATAAAAATGC GGGCTGGTTT 
GGCGGACTCT GGAACAAGTT GTCGCTAAAG CCGAAGAACC AAATGATCCT GCCGGATGAC 
AAGAATCCGG CCATCGTCTG GGACAAGGAG CGCAAATGCT GGACAAACAC CGAAGGCAAC 
GGCGACGAGG CTGAAAGCTT TAAGCCGCCA CCCAAAATGA GTGATCTGGG CATGGCTCTG 
GGAGGACCAC CCGCTGCGCC AATCGGTAAT GCCGGATTGG GAATGGGTTT AATGCCTGTG 
GCTAACAATG CGCCGGGCAG CCATCAATCA GCACCGTTGA TGGATCAGCA AACGCCGTCT 
CAGCCGCAAA CGTATGGCAG CCCCATTGAT TACACGGCTG CTCCGGCACC CGAGCTGATT 
CCAACGGTAC CATCGCCGGC TCCACTTTCT GTCCCGTCAT CTGCACCGGC GGCATTAGCG 
GCGCCCAATC CCGCGGCCGT TCCCGGCGGA CCGCAGCCCA AGCTGCAGTC GAACATGTTC 
AAGATGCAAC GCAACCGCAC GCTAAAGAAC TCCTACGTGG ATGTGTTCAA TCCTTCGGGT 
GCGCCAATGT CGGCGGCACC GGAGAATGTG CTGGCCCCCA TGATGGCGCC GGCTGCGGTG 
CCCCAAGGCG GTTACTTTGT ACCCCAGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec16 ( NM_001103493.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096963.1 Drosophila melanogaster secretory 16, transcript variant E (Sec16), mRNA.

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CloneID	OFa01411
Clone ID Related Accession (Same CDS sequence)	NM_001103493.4
Accession Version	NM_001103493.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 16, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103493.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGA GCTGCTGCCG ATGCGGCACC TTCGACTTAT 
GGAGGAGTGG CTGGAACCGC TGGACCGGTG ACCAACACTG CGGTGGCTCG TCCGCCGCGC 
AGACGCACTC CCTTGCAGTT CAGCCGACCG CATTTGGTGG CCTCGTATGC GATGAGTCTG 
CTGCTGAAGG TGAAGCCCAA ATACGCGGGA CGTGGACGAC TGCGCAACGA TGTGGAGGTG 
GCACCACCAC GCATACGGGA CGGTACGAGC AGCCTGCTGC GCATGTATCC TGGTCCCTTG 
CAGGGACGCA AGTTGCACAA GGACAAGATC ATCAGTTTCT GCAAGGAGCA GATCCGACTG 
GGGCCCACAA AGGGTTGCAC CGCACTGTAT GCCACGCAGA AGAAGCCACA GGGCAGCGTG 
ACGAAATACC GTGCCTCCCA TGCGCTCATG TGGCACCTGC TAATACTTTT GCTGCGCCAA 
AATGGGTACA TTGCGGACAC GGATGTGGGC GATCTGCTGC TGGAGAACCA GCAAGAGTAT 
CCCTATGATC CCAGCGAGTT CGAAGCAGAG AACGAGCCAG ATGCGGATGC CGAACAGGAT 
GCCGCTGCAC CGGCGGACAA GACAGTCGAT TCGGATTTAG ATAGCGAATC GGCAGCAGGA 
GTTACACCTG CGGAACCACT TGCAGCAGGC GCGGCAACTA GCTCAATCAA TGGAGCTGAT 
GCTGCAAACG CAGCAACGCC TCTGTCGGAA CAGGCGGCAA CGGACAAGTT CCGCAGCTAT 
GTGCTGCGCG GAAATGTCGA GGAGGCTCTC CAGTGGGCAA CCGACAACGG CCTGTGGACG 
CATGCATTCT TCTTGGCCCT CTACGAGGAT CGCTATGCTC TCACTGACGT AGCCCAGAAG 
TTCCTCAATC GAGCGATCAA GGCCAACGAT CCATTGCAGA CACTATACCA AATGAAGAGC 
TGCCACACAC CGGCGTGCGT CAGCCAGCTG AGGGATGAAC AGTGGGGTGA CTGGCGATCG 
CATCTCTCCA TCCTGGTGAC GAACAAGAGT CGCCAGCCGG AGTATGATCG CAGTTCGGTA 
GTGGCCCTTG GCGATACGCT TTTCCAGCGC GGCGACATTT ATGCGGCACA CTTTTGCTAT 
CTGGTGGCTC AGGAGGAGTT CGGACGATAC GATAGCTCTG CTACAGAGCT AACTACTCTG 
ACCGCCAATG TGCCCAGGCT TATTCTACTT GGCTCCTCGC ACTACAAGCA CTTCAACGAA 
TTTGCCAGCA ACGAGGCGAT CATCATGACC GAGATCTATG AGTATGCTCG ATCGCTGTTC 
GATCCGAAGT TTAGCATTGC CCACTTCCAA CACTACAAGT TCCTGCTAGC CACAAGGATC 
CTAGATTATG GTCAGCATTT CCGCTGCACC AATTATTTGG AACAAATCGC CAGGCACATC 
GAACTGAAGC CGGAAAGCTA CGACAGTAAT TTCATTCAGC GGGTTTGCGG TTTGGCCGAA 
CGCCTGCGCT ACCATGATCC CATTCTCATT AATCGTGTCT CATTTGCGAG TCCTCCGAAT 
GCCACTAGCA AGGATAGTGC TGCTGCTGAA GAGAAGGCCT GGTTGCGTCA GCTTAGATCT 
CTGGCCTATG TGCAGCCCCA ACAAGAGCAG CTGCAACAAC TGCAGCAGAA TCAGCAAGTC 
CAGCAGGAGC AAAACGACAT CGATCAGCAG TTTGCAGAGG TGAACAAGCA ATTCAGGGAG 
CTAAACATGC AATACGAGAG CAACAGCAAC ATGGAGAATA CGCTGCAGCC GCAGTTGCCA 
CCGGTGGATC AACAGCCGCC GCAACAGCAG CAACAAGTTT TATCGGAATC GTACCAACAG 
CCAACACAAC AATATTATGA GCCACCACCT CAAGCGCCAA CCGAATCCGA TCCCTATGGA 
CAGGGCCAAC CGTCCTATTA TGATCCCAAT GCGGGGCAGC TTCATTACGA TCCAAATGCT 
GCACAGCATC AATACGACCA GCAGCAACCT CAACCACAGC CGACTCCCAG CTATGGCCAA 
ATAGAGCCAG GAACTGAGGC CTACCAACCT GGACAAGCAA CAGCAGATCC AGCTGCGGTT 
CCCTCTGGCT ACGGATACGA TTACTGGTCG GGCACACAGC AGCCACCTTA TGGTGACGAG 
CTGCAGCAAC AGACGCGCCC GGCGATATCC ATGCCCAAGT CAAAGAGCTA CGGAGATGAG 
GACGATGGTG GTTCCGGTGG TGTGGCTGAT CCTGGTCAGA AAACCAAGCC TGTAGGCTCC 
TCTGAGGCTG CAGGCAAACA GCAGGCGTCG GGCAAACAGG CCAACTCCGG CAGCGATCTT 
GGCGCACCTG GGAATAAAAA TGCGGGCTGG TTTGGCGGAC TCTGGAACAA GTTGTCGCTA 
AAGCCGAAGA ACCAAATGAT CCTGCCGGAT GACAAGAATC CGGCCATCGT CTGGGACAAG 
GAGCGCAAAT GCTGGACAAA CACCGAAGGC AACGGCGACG AGGCTGAAAG CTTTAAGCCG 
CCACCCAAAA TGAGTGATCT GGGCATGGCT CTGGGAGGAC CACCCGCTGC GCCAATCGGT 
AATGCCGGAT TGGGAATGGG TTTAATGCCT GTGGCTAACA ATGCGCCGGG CAGCCATCAA 
TCAGCACCGT TGATGGATCA GCAAACGCCG TCTCAGCCGC AAACGTATGG CAGCCCCATT 
GATTACACGG CTGCTCCGGC ACCCGAGCTG ATTCCAACGG TACCATCGCC GGCTCCACTT 
TCTGTCCCGT CATCTGCACC GGCGGCATTA GCGGCGCCCA ATCCCGCGGC CGTTCCCGGC 
GGACCGCAGC CCAAGCTGCA GTCGAACATG TTCAAGATGC AACGCAACCG CACGCTAAAG 
AACTCCTACG TGGATGTGTT CAATCCTTCG GGTGCGCCAA TGTCGGCGGC ACCGGAGAAT 
GTGCTGGCCC CCATGATGGC GCCGGCTGCG GTGCCCCAAG GCGGTTACTT TGTACCCCAG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096963.1
CDS	77..6142
Translation	MLHNNAPWLPPHQQQQQQAPQQHPTPQQQHAMPQQQQTLPAQQQQQQVYASQMFQQHQPNYWPEDQHQQQQQPQSLNYNNYFPGQQQQHPMQQQQLPPQQQLPPQQQQPTQQQHTIQQQLYYPTQQQPQVPAPAEPALDSFDNNNSGGGGGGGRSDGWGDWGDWNDNSNNNNSNGTDGLLEPTGQLLEDSFNVQSSQGSWQAFATSNNVNNNGELPPPANPQSTSLQQQPLNQQSIPLLHQQPQSPPELGQEPELDAIVPPQAFQNQPPTAAPPPTSLTSFSAQMTAVGSPIYAVGASSAHSAGVGASPAPPTGVVAPPAHLAGIGAPPAHLAGVGASPAPSALVGASPAPIIGVGAPPVGAPPVGAPPADATTIAPPAALPPSIVPSSGSNPFKRSTGLNKRVNIMADPAAGAPSPPAPAAAIAPVAPVAPIPPPADQLFGMPAEAHGEGFNLIAAPPVEASLGTPLSAPIPAPIPVPNASLYASPAVPQAFAHLEPDNQEVLSAPNDERAQYLQTSHLSEQLGEGEADQDAGLLPPPGLSRLVLGQPELDSQQQRQVTGATEQPPLNVAQAAALHMQERRADGEDTSDGEQQVRNIQTPPRRVVTGVETNAPSLREQREVVLDGENLEDREAIPPPTLAELPTTSVHHNILPDEAEQLHHNNPPQAMTPLNAQQPHQQQSTLQQQHQQHSTTQQEKKRAAVGRRTTASLDLESDESDEFLQSERERDRERERRDLMEERHSRGRSHSHYPYEGETEDSVRGAAHHETKSLRETHHKRNHDSARSRRHQDPKVERERERDRERDRTWRRRSNKYHSGGEDPDRSYDHSRRYNNSNYEGESDNPEYNHMGDAELDGSGGRSSKTSRHRRSAAEEDFDDYERERNRSRRSTKPQSSAEKSRSSGGRRNYENSGRSARADDGRRRYQDQRNPGAQYPVSATGYVPYGMYEQMSRNPQVYADMYAKFYGQMINSMNAAVSAAASKGGVPAGAGVIPGLMPSAVPVSAAQLVAATSGGSVSGSSEAAMLRERERAAADAAPSTYGGVAGTAGPVTNTAVARPPRRRTPLQFSRPHLVASYAMSLLLKVKPKYAGRGRLRNDVEVAPPRIRDGTSSLLRMYPGPLQGRKLHKDKIISFCKEQIRLGPTKGCTALYATQKKPQGSVTKYRASHALMWHLLILLLRQNGYIADTDVGDLLLENQQEYPYDPSEFEAENEPDADAEQDAAAPADKTVDSDLDSESAAGVTPAEPLAAGAATSSINGADAANAATPLSEQAATDKFRSYVLRGNVEEALQWATDNGLWTHAFFLALYEDRYALTDVAQKFLNRAIKANDPLQTLYQMKSCHTPACVSQLRDEQWGDWRSHLSILVTNKSRQPEYDRSSVVALGDTLFQRGDIYAAHFCYLVAQEEFGRYDSSATELTTLTANVPRLILLGSSHYKHFNEFASNEAIIMTEIYEYARSLFDPKFSIAHFQHYKFLLATRILDYGQHFRCTNYLEQIARHIELKPESYDSNFIQRVCGLAERLRYHDPILINRVSFASPPNATSKDSAAAEEKAWLRQLRSLAYVQPQQEQLQQLQQNQQVQQEQNDIDQQFAEVNKQFRELNMQYESNSNMENTLQPQLPPVDQQPPQQQQQVLSESYQQPTQQYYEPPPQAPTESDPYGQGQPSYYDPNAGQLHYDPNAAQHQYDQQQPQPQPTPSYGQIEPGTEAYQPGQATADPAAVPSGYGYDYWSGTQQPPYGDELQQQTRPAISMPKSKSYGDEDDGGSGGVADPGQKTKPVGSSEAAGKQQASGKQANSGSDLGAPGNKNAGWFGGLWNKLSLKPKNQMILPDDKNPAIVWDKERKCWTNTEGNGDEAESFKPPPKMSDLGMALGGPPAAPIGNAGLGMGLMPVANNAPGSHQSAPLMDQQTPSQPQTYGSPIDYTAAPAPELIPTVPSPAPLSVPSSAPAALAAPNPAAVPGGPQPKLQSNMFKMQRNRTLKNSYVDVFNPSGAPMSAAPENVLAPMMAPAAVPQGGYFVPQQ

Target ORF information:

RefSeq Version	NM_001103493.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 16, transcript variant E (Sec16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103493.4

ORF Insert Sequence:

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGA GCTGCTGCCG ATGCGGCACC TTCGACTTAT 
GGAGGAGTGG CTGGAACCGC TGGACCGGTG ACCAACACTG CGGTGGCTCG TCCGCCGCGC 
AGACGCACTC CCTTGCAGTT CAGCCGACCG CATTTGGTGG CCTCGTATGC GATGAGTCTG 
CTGCTGAAGG TGAAGCCCAA ATACGCGGGA CGTGGACGAC TGCGCAACGA TGTGGAGGTG 
GCACCACCAC GCATACGGGA CGGTACGAGC AGCCTGCTGC GCATGTATCC TGGTCCCTTG 
CAGGGACGCA AGTTGCACAA GGACAAGATC ATCAGTTTCT GCAAGGAGCA GATCCGACTG 
GGGCCCACAA AGGGTTGCAC CGCACTGTAT GCCACGCAGA AGAAGCCACA GGGCAGCGTG 
ACGAAATACC GTGCCTCCCA TGCGCTCATG TGGCACCTGC TAATACTTTT GCTGCGCCAA 
AATGGGTACA TTGCGGACAC GGATGTGGGC GATCTGCTGC TGGAGAACCA GCAAGAGTAT 
CCCTATGATC CCAGCGAGTT CGAAGCAGAG AACGAGCCAG ATGCGGATGC CGAACAGGAT 
GCCGCTGCAC CGGCGGACAA GACAGTCGAT TCGGATTTAG ATAGCGAATC GGCAGCAGGA 
GTTACACCTG CGGAACCACT TGCAGCAGGC GCGGCAACTA GCTCAATCAA TGGAGCTGAT 
GCTGCAAACG CAGCAACGCC TCTGTCGGAA CAGGCGGCAA CGGACAAGTT CCGCAGCTAT 
GTGCTGCGCG GAAATGTCGA GGAGGCTCTC CAGTGGGCAA CCGACAACGG CCTGTGGACG 
CATGCATTCT TCTTGGCCCT CTACGAGGAT CGCTATGCTC TCACTGACGT AGCCCAGAAG 
TTCCTCAATC GAGCGATCAA GGCCAACGAT CCATTGCAGA CACTATACCA AATGAAGAGC 
TGCCACACAC CGGCGTGCGT CAGCCAGCTG AGGGATGAAC AGTGGGGTGA CTGGCGATCG 
CATCTCTCCA TCCTGGTGAC GAACAAGAGT CGCCAGCCGG AGTATGATCG CAGTTCGGTA 
GTGGCCCTTG GCGATACGCT TTTCCAGCGC GGCGACATTT ATGCGGCACA CTTTTGCTAT 
CTGGTGGCTC AGGAGGAGTT CGGACGATAC GATAGCTCTG CTACAGAGCT AACTACTCTG 
ACCGCCAATG TGCCCAGGCT TATTCTACTT GGCTCCTCGC ACTACAAGCA CTTCAACGAA 
TTTGCCAGCA ACGAGGCGAT CATCATGACC GAGATCTATG AGTATGCTCG ATCGCTGTTC 
GATCCGAAGT TTAGCATTGC CCACTTCCAA CACTACAAGT TCCTGCTAGC CACAAGGATC 
CTAGATTATG GTCAGCATTT CCGCTGCACC AATTATTTGG AACAAATCGC CAGGCACATC 
GAACTGAAGC CGGAAAGCTA CGACAGTAAT TTCATTCAGC GGGTTTGCGG TTTGGCCGAA 
CGCCTGCGCT ACCATGATCC CATTCTCATT AATCGTGTCT CATTTGCGAG TCCTCCGAAT 
GCCACTAGCA AGGATAGTGC TGCTGCTGAA GAGAAGGCCT GGTTGCGTCA GCTTAGATCT 
CTGGCCTATG TGCAGCCCCA ACAAGAGCAG CTGCAACAAC TGCAGCAGAA TCAGCAAGTC 
CAGCAGGAGC AAAACGACAT CGATCAGCAG TTTGCAGAGG TGAACAAGCA ATTCAGGGAG 
CTAAACATGC AATACGAGAG CAACAGCAAC ATGGAGAATA CGCTGCAGCC GCAGTTGCCA 
CCGGTGGATC AACAGCCGCC GCAACAGCAG CAACAAGTTT TATCGGAATC GTACCAACAG 
CCAACACAAC AATATTATGA GCCACCACCT CAAGCGCCAA CCGAATCCGA TCCCTATGGA 
CAGGGCCAAC CGTCCTATTA TGATCCCAAT GCGGGGCAGC TTCATTACGA TCCAAATGCT 
GCACAGCATC AATACGACCA GCAGCAACCT CAACCACAGC CGACTCCCAG CTATGGCCAA 
ATAGAGCCAG GAACTGAGGC CTACCAACCT GGACAAGCAA CAGCAGATCC AGCTGCGGTT 
CCCTCTGGCT ACGGATACGA TTACTGGTCG GGCACACAGC AGCCACCTTA TGGTGACGAG 
CTGCAGCAAC AGACGCGCCC GGCGATATCC ATGCCCAAGT CAAAGAGCTA CGGAGATGAG 
GACGATGGTG GTTCCGGTGG TGTGGCTGAT CCTGGTCAGA AAACCAAGCC TGTAGGCTCC 
TCTGAGGCTG CAGGCAAACA GCAGGCGTCG GGCAAACAGG CCAACTCCGG CAGCGATCTT 
GGCGCACCTG GGAATAAAAA TGCGGGCTGG TTTGGCGGAC TCTGGAACAA GTTGTCGCTA 
AAGCCGAAGA ACCAAATGAT CCTGCCGGAT GACAAGAATC CGGCCATCGT CTGGGACAAG 
GAGCGCAAAT GCTGGACAAA CACCGAAGGC AACGGCGACG AGGCTGAAAG CTTTAAGCCG 
CCACCCAAAA TGAGTGATCT GGGCATGGCT CTGGGAGGAC CACCCGCTGC GCCAATCGGT 
AATGCCGGAT TGGGAATGGG TTTAATGCCT GTGGCTAACA ATGCGCCGGG CAGCCATCAA 
TCAGCACCGT TGATGGATCA GCAAACGCCG TCTCAGCCGC AAACGTATGG CAGCCCCATT 
GATTACACGG CTGCTCCGGC ACCCGAGCTG ATTCCAACGG TACCATCGCC GGCTCCACTT 
TCTGTCCCGT CATCTGCACC GGCGGCATTA GCGGCGCCCA ATCCCGCGGC CGTTCCCGGC 
GGACCGCAGC CCAAGCTGCA GTCGAACATG TTCAAGATGC AACGCAACCG CACGCTAAAG 
AACTCCTACG TGGATGTGTT CAATCCTTCG GGTGCGCCAA TGTCGGCGGC ACCGGAGAAT 
GTGCTGGCCC CCATGATGGC GCCGGCTGCG GTGCCCCAAG GCGGTTACTT TGTACCCCAG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec16 ( NM_001298230.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285159.1 Drosophila melanogaster secretory 16, transcript variant J (Sec16), mRNA.

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CloneID	OFa21444
Clone ID Related Accession (Same CDS sequence)	NM_001298230.1
Accession Version	NM_001298230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 16, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGG TATACACACG CATACATAAC CCAAGCCAAT 
GAATTCCATC GTCACCAATA CAAAGAGCTG ATCTACCAGC AACAACAACA ACAACAGCAG 
CAGCAACAAC AACAACAGCA GAGGGAGGAT CACCTGAACT CCAGCTTTAG CGTTGCAGAG 
GATAATGCCA GTTTTTACGG ATCGCACGGA GGAGGTTCCA TCTATAACCA ATACCAATCG 
TATCCACTAT CCAGTGCCCG ATCGTTGAGT AACTTGAACG GCGATGGACG CAATTCCCGA 
TGCGGACCGT ATTACGCTGG TTCCGAATGC GGTCTCGATA TCAGAGCTGC TGCCGATGCG 
GCACCTTCGA CTTATGGAGG AGTGGCTGGA ACCGCTGGAC CGGTGACCAA CACTGCGGTG 
GCTCGTCCGC CGCGCAGACG CACTCCCTTG CAGTTCAGCC GACCGCATTT GGTGGCCTCG 
TATGCGATGA GTCTGCTGCT GAAGGTGAAG CCCAAATACG CGGGACGTGG ACGACTGCGC 
AACGATGTGG AGGTGGCACC ACCACGCATA CGGGACGGTA CGAGCAGCCT GCTGCGCATG 
TATCCTGGTC CCTTGCAGGG ACGCAAGTTG CACAAGGACA AGATCATCAG TTTCTGCAAG 
GAGCAGATCC GACTGGGGCC CACAAAGGGT TGCACCGCAC TGTATGCCAC GCAGAAGAAG 
CCACAGGGCA GCGTGACGAA ATACCGTGCC TCCCATGCGC TCATGTGGCA CCTGCTAATA 
CTTTTGCTGC GCCAAAATGG GTACATTGCG GACACGGATG TGGGCGATCT GCTGCTGGAG 
AACCAGCAAG AGTATCCCTA TGATCCCAGC GAGTTCGAAG CAGAGAACGA GCCAGATGCG 
GATGCCGAAC AGGATGCCGC TGCACCGGCG GACAAGACAG TCGATTCGGA TTTAGATAGC 
GAATCGGCAG CAGGAGTTAC ACCTGCGGAA CCACTTGCAG CAGGCGCGGC AACTAGCTCA 
ATCAATGGAG CTGATGCTGC AAACGCAGCA ACGCCTCTGT CGGAACAGGC GGCAACGGAC 
AAGTTCCGCA GCTATGTGCT GCGCGGAAAT GTCGAGGAGG CTCTCCAGTG GGCAACCGAC 
AACGGCCTGT GGACGCATGC ATTCTTCTTG GCCCTCTACG AGGATCGCTA TGCTCTCACT 
GACGTAGCCC AGAAGTTCCT CAATCGAGCG ATCAAGGCCA ACGATCCATT GCAGACACTA 
TACCAAATGA AGAGCTGCCA CACACCGGCG TGCGTCAGCC AGCTGAGGGA TGAACAGTGG 
GGTGACTGGC GATCGCATCT CTCCATCCTG GTGACGAACA AGAGTCGCCA GCCGGAGTAT 
GATCGCAGTT CGGTAGTGGC CCTTGGCGAT ACGCTTTTCC AGCGCGGCGA CATTTATGCG 
GCACACTTTT GCTATCTGGT GGCTCAGGAG GAGTTCGGAC GATACGATAG CTCTGCTACA 
GAGCTAACTA CTCTGACCGC CAATGTGCCC AGGCTTATTC TACTTGGCTC CTCGCACTAC 
AAGCACTTCA ACGAATTTGC CAGCAACGAG GCGATCATCA TGACCGAGAT CTATGAGTAT 
GCTCGATCGC TGTTCGATCC GAAGTTTAGC ATTGCCCACT TCCAACACTA CAAGTTCCTG 
CTAGCCACAA GGATCCTAGA TTATGGTCAG CATTTCCGCT GCACCAATTA TTTGGAACAA 
ATCGCCAGGC ACATCGAACT GAAGCCGGAA AGCTACGACA GTAATTTCAT TCAGCGGGTT 
TGCGGTTTGG CCGAACGCCT GCGCTACCAT GATCCCATTC TCATTAATCG TGTCTCATTT 
GCGAGTCCTC CGAATGCCAC TAGCAAGGAT AGTGCTGCTG CTGAAGAGAA GGCCTGGTTG 
CGTCAGCTTA GATCTCTGGC CTATGTGCCC CAACAAGAGC AGCTGCAACA ACTGCAGCAG 
AATCAGCAAG TCCAGCAGGA GCAAAACGAC ATCGATCAGC AGTTTGCAGA GGTGAACAAG 
CAATTCAGGG AGCTAAACAT GCAATACGAG AGCAACAGCA ACATGGAGAA TACGCTGCAG 
CCGCAGTTGC CACCGGTGGA TCAACAGCCG CCGCAACAGC AGCAACAAGT TTTATCGGAA 
TCGTACCAAC AGCCAACACA ACAATATTAT GAGCCACCAC CTCAAGCGCC AACCGAATCC 
GATCCCTATG GACAGGGCCA ACCGTCCTAT TATGATCCCA ATGCGGGGCA GCTTCATTAC 
GATCCAAATG CTGCACAGCA TCAATACGAC CAGCAGCAAC CTCAACCACA GCCGACTCCC 
AGCTATGGCC AAATAGAGCC AGGAACTGAG GCCTACCAAC CTGGACAAGC AACAGCAGAT 
CCAGCTGCGG TTCCCTCTGG CTACGGATAC GATTACTGGT CGGGCACACA GCAGCCACCT 
TATGGTGACG AGCCCAATGA GAATCAAGCT GCACTAAAGG ATGAAGAGGA GGAGGATGAG 
GAGCAGGATG AACAGCAGAT ATTGCAGTAC GCTAAACAGC AGATTTTGCA GCAAACCAAG 
CAGCAGCAGC TGCAGCAGGA GCAACATACT CGCAGCAATG GCGCCGCCAG CATCAACAAT 
CGCTTCAAAT CGAACGCTCT GAAGCAGGAA CGCAGCAGCG GCTCATTGCG GAAAAGGCAA 
GAGGCATCAC GAACAGCAAC AATAGCAGCG GCAGCAGCAG CTATTGCTCA GATAACACCG 
GCAACAGTTG CAGCGGCAGC GACGCCACCA GCAGCATCGG CAAAGGCATT CAATTTGAAT 
GATCTGCAGC AACAGACGCG CCCGGCGATA TCCATGCCCA AGTCAAAGAG CTACGGAGAT 
GAGGACGATG GTGGTTCCGG TGGTGTGGCT GATCCTGGTC AGAAAACCAA GCCTGTAGGC 
TCCTCTGAGG CTGCAGGCAA ACAGCAGGCG TCGGGCAAAC AGGCCAACTC CGGCAGCGAT 
CTTGGCGCAC CTGGGAATAA AAATGCGGGC TGGTTTGGCG GACTCTGGAA CAAGTTGTCG 
CTAAAGCCGA AGAACCAAAT GATCCTGCCG GATGACAAGA ATCCGGCCAT CGTCTGGGAC 
AAGGAGCGCA AATGCTGGAC AAACACCGAA GGCAACGGCG ACGAGGCTGA AAGCTTTAAG 
CCGCCACCCA AAATGAGTGA TCTGGGCATG GCTCTGGGAG GACCACCCGC TGCGCCAATC 
GGTAATGCCG GATTGGGAAT GGGTTTAATG CCTGTGGCTA ACAATGCGCC GGGCAGCCAT 
CAATCAGCAC CGTTGATGGA TCAGCAAACG CCGTCTCAGC CGCAAACGTA TGGCAGCCCC 
ATTGATTACA CGGCTGCTCC GGCACCCGAG CTGATTCCAA CGGTACCATC GCCGGCTCCA 
CTTTCTGTCC CGTCATCTGC ACCGGCGGCA TTAGCGGCGC CCAATCCCGC GGCCGTTCCC 
GGCGGACCGC AGCCCAAGCT GCAGTCGAAC ATGTTCAAGA TGCAACGCAA CCGCACGCTA 
AAGAACTCCT ACGTGGATGT GTTCAATCCT TCGGGTGCGC CAATGTCGGC GGCACCGGAG 
AATGTGCTGG CCCCCATGAT GGCGCCGGCT GCGGTGCCCC AAGGCGGTTA CTTTGTACCC 
CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285159.1
CDS	196..6924
Translation	MLHNNAPWLPPHQQQQQQAPQQHPTPQQQHAMPQQQQTLPAQQQQQQVYASQMFQQHQPNYWPEDQHQQQQQPQSLNYNNYFPGQQQQHPMQQQQLPPQQQLPPQQQQPTQQQHTIQQQLYYPTQQQPQVPAPAEPALDSFDNNNSGGGGGGGRSDGWGDWGDWNDNSNNNNSNGTDGLLEPTGQLLEDSFNVQSSQGSWQAFATSNNVNNNGELPPPANPQSTSLQQQPLNQQSIPLLHQQPQSPPELGQEPELDAIVPPQAFQNQPPTAAPPPTSLTSFSAQMTAVGSPIYAVGASSAHSAGVGASPAPPTGVVAPPAHLAGIGAPPAHLAGVGASPAPSALVGASPAPIIGVGAPPVGAPPVGAPPADATTIAPPAALPPSIVPSSGSNPFKRSTGLNKRVNIMADPAAGAPSPPAPAAAIAPVAPVAPIPPPADQLFGMPAEAHGEGFNLIAAPPVEASLGTPLSAPIPAPIPVPNASLYASPAVPQAFAHLEPDNQEVLSAPNDERAQYLQTSHLSEQLGEGEADQDAGLLPPPGLSRLVLGQPELDSQQQRQVTGATEQPPLNVAQAAALHMQERRADGEDTSDGEQQVRNIQTPPRRVVTGVETNAPSLREQREVVLDGENLEDREAIPPPTLAELPTTSVHHNILPDEAEQLHHNNPPQAMTPLNAQQPHQQQSTLQQQHQQHSTTQQEKKRAAVGRRTTASLDLESDESDEFLQSERERDRERERRDLMEERHSRGRSHSHYPYEGETEDSVRGAAHHETKSLRETHHKRNHDSARSRRHQDPKVERERERDRERDRTWRRRSNKYHSGGEDPDRSYDHSRRYNNSNYEGESDNPEYNHMGDAELDGSGGRSSKTSRHRRSAAEEDFDDYERERNRSRRSTKPQSSAEKSRSSGGRRNYENSGRSARADDGRRRYQDQRNPGAQYPVSATGYVPYGMYEQMSRNPQVYADMYAKFYGQMINSMNAAVSAAASKGGVPAGAGVIPGLMPSAVPVSAAQLVAATSGGSVSGSSEAAMLRERERYTHAYITQANEFHRHQYKELIYQQQQQQQQQQQQQQQREDHLNSSFSVAEDNASFYGSHGGGSIYNQYQSYPLSSARSLSNLNGDGRNSRCGPYYAGSECGLDIRAAADAAPSTYGGVAGTAGPVTNTAVARPPRRRTPLQFSRPHLVASYAMSLLLKVKPKYAGRGRLRNDVEVAPPRIRDGTSSLLRMYPGPLQGRKLHKDKIISFCKEQIRLGPTKGCTALYATQKKPQGSVTKYRASHALMWHLLILLLRQNGYIADTDVGDLLLENQQEYPYDPSEFEAENEPDADAEQDAAAPADKTVDSDLDSESAAGVTPAEPLAAGAATSSINGADAANAATPLSEQAATDKFRSYVLRGNVEEALQWATDNGLWTHAFFLALYEDRYALTDVAQKFLNRAIKANDPLQTLYQMKSCHTPACVSQLRDEQWGDWRSHLSILVTNKSRQPEYDRSSVVALGDTLFQRGDIYAAHFCYLVAQEEFGRYDSSATELTTLTANVPRLILLGSSHYKHFNEFASNEAIIMTEIYEYARSLFDPKFSIAHFQHYKFLLATRILDYGQHFRCTNYLEQIARHIELKPESYDSNFIQRVCGLAERLRYHDPILINRVSFASPPNATSKDSAAAEEKAWLRQLRSLAYVPQQEQLQQLQQNQQVQQEQNDIDQQFAEVNKQFRELNMQYESNSNMENTLQPQLPPVDQQPPQQQQQVLSESYQQPTQQYYEPPPQAPTESDPYGQGQPSYYDPNAGQLHYDPNAAQHQYDQQQPQPQPTPSYGQIEPGTEAYQPGQATADPAAVPSGYGYDYWSGTQQPPYGDEPNENQAALKDEEEEDEEQDEQQILQYAKQQILQQTKQQQLQQEQHTRSNGAASINNRFKSNALKQERSSGSLRKRQEASRTATIAAAAAAIAQITPATVAAAATPPAASAKAFNLNDLQQQTRPAISMPKSKSYGDEDDGGSGGVADPGQKTKPVGSSEAAGKQQASGKQANSGSDLGAPGNKNAGWFGGLWNKLSLKPKNQMILPDDKNPAIVWDKERKCWTNTEGNGDEAESFKPPPKMSDLGMALGGPPAAPIGNAGLGMGLMPVANNAPGSHQSAPLMDQQTPSQPQTYGSPIDYTAAPAPELIPTVPSPAPLSVPSSAPAALAAPNPAAVPGGPQPKLQSNMFKMQRNRTLKNSYVDVFNPSGAPMSAAPENVLAPMMAPAAVPQGGYFVPQQ

Target ORF information:

RefSeq Version	NM_001298230.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 16, transcript variant J (Sec16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298230.1

ORF Insert Sequence:

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGG TATACACACG CATACATAAC CCAAGCCAAT 
GAATTCCATC GTCACCAATA CAAAGAGCTG ATCTACCAGC AACAACAACA ACAACAGCAG 
CAGCAACAAC AACAACAGCA GAGGGAGGAT CACCTGAACT CCAGCTTTAG CGTTGCAGAG 
GATAATGCCA GTTTTTACGG ATCGCACGGA GGAGGTTCCA TCTATAACCA ATACCAATCG 
TATCCACTAT CCAGTGCCCG ATCGTTGAGT AACTTGAACG GCGATGGACG CAATTCCCGA 
TGCGGACCGT ATTACGCTGG TTCCGAATGC GGTCTCGATA TCAGAGCTGC TGCCGATGCG 
GCACCTTCGA CTTATGGAGG AGTGGCTGGA ACCGCTGGAC CGGTGACCAA CACTGCGGTG 
GCTCGTCCGC CGCGCAGACG CACTCCCTTG CAGTTCAGCC GACCGCATTT GGTGGCCTCG 
TATGCGATGA GTCTGCTGCT GAAGGTGAAG CCCAAATACG CGGGACGTGG ACGACTGCGC 
AACGATGTGG AGGTGGCACC ACCACGCATA CGGGACGGTA CGAGCAGCCT GCTGCGCATG 
TATCCTGGTC CCTTGCAGGG ACGCAAGTTG CACAAGGACA AGATCATCAG TTTCTGCAAG 
GAGCAGATCC GACTGGGGCC CACAAAGGGT TGCACCGCAC TGTATGCCAC GCAGAAGAAG 
CCACAGGGCA GCGTGACGAA ATACCGTGCC TCCCATGCGC TCATGTGGCA CCTGCTAATA 
CTTTTGCTGC GCCAAAATGG GTACATTGCG GACACGGATG TGGGCGATCT GCTGCTGGAG 
AACCAGCAAG AGTATCCCTA TGATCCCAGC GAGTTCGAAG CAGAGAACGA GCCAGATGCG 
GATGCCGAAC AGGATGCCGC TGCACCGGCG GACAAGACAG TCGATTCGGA TTTAGATAGC 
GAATCGGCAG CAGGAGTTAC ACCTGCGGAA CCACTTGCAG CAGGCGCGGC AACTAGCTCA 
ATCAATGGAG CTGATGCTGC AAACGCAGCA ACGCCTCTGT CGGAACAGGC GGCAACGGAC 
AAGTTCCGCA GCTATGTGCT GCGCGGAAAT GTCGAGGAGG CTCTCCAGTG GGCAACCGAC 
AACGGCCTGT GGACGCATGC ATTCTTCTTG GCCCTCTACG AGGATCGCTA TGCTCTCACT 
GACGTAGCCC AGAAGTTCCT CAATCGAGCG ATCAAGGCCA ACGATCCATT GCAGACACTA 
TACCAAATGA AGAGCTGCCA CACACCGGCG TGCGTCAGCC AGCTGAGGGA TGAACAGTGG 
GGTGACTGGC GATCGCATCT CTCCATCCTG GTGACGAACA AGAGTCGCCA GCCGGAGTAT 
GATCGCAGTT CGGTAGTGGC CCTTGGCGAT ACGCTTTTCC AGCGCGGCGA CATTTATGCG 
GCACACTTTT GCTATCTGGT GGCTCAGGAG GAGTTCGGAC GATACGATAG CTCTGCTACA 
GAGCTAACTA CTCTGACCGC CAATGTGCCC AGGCTTATTC TACTTGGCTC CTCGCACTAC 
AAGCACTTCA ACGAATTTGC CAGCAACGAG GCGATCATCA TGACCGAGAT CTATGAGTAT 
GCTCGATCGC TGTTCGATCC GAAGTTTAGC ATTGCCCACT TCCAACACTA CAAGTTCCTG 
CTAGCCACAA GGATCCTAGA TTATGGTCAG CATTTCCGCT GCACCAATTA TTTGGAACAA 
ATCGCCAGGC ACATCGAACT GAAGCCGGAA AGCTACGACA GTAATTTCAT TCAGCGGGTT 
TGCGGTTTGG CCGAACGCCT GCGCTACCAT GATCCCATTC TCATTAATCG TGTCTCATTT 
GCGAGTCCTC CGAATGCCAC TAGCAAGGAT AGTGCTGCTG CTGAAGAGAA GGCCTGGTTG 
CGTCAGCTTA GATCTCTGGC CTATGTGCCC CAACAAGAGC AGCTGCAACA ACTGCAGCAG 
AATCAGCAAG TCCAGCAGGA GCAAAACGAC ATCGATCAGC AGTTTGCAGA GGTGAACAAG 
CAATTCAGGG AGCTAAACAT GCAATACGAG AGCAACAGCA ACATGGAGAA TACGCTGCAG 
CCGCAGTTGC CACCGGTGGA TCAACAGCCG CCGCAACAGC AGCAACAAGT TTTATCGGAA 
TCGTACCAAC AGCCAACACA ACAATATTAT GAGCCACCAC CTCAAGCGCC AACCGAATCC 
GATCCCTATG GACAGGGCCA ACCGTCCTAT TATGATCCCA ATGCGGGGCA GCTTCATTAC 
GATCCAAATG CTGCACAGCA TCAATACGAC CAGCAGCAAC CTCAACCACA GCCGACTCCC 
AGCTATGGCC AAATAGAGCC AGGAACTGAG GCCTACCAAC CTGGACAAGC AACAGCAGAT 
CCAGCTGCGG TTCCCTCTGG CTACGGATAC GATTACTGGT CGGGCACACA GCAGCCACCT 
TATGGTGACG AGCCCAATGA GAATCAAGCT GCACTAAAGG ATGAAGAGGA GGAGGATGAG 
GAGCAGGATG AACAGCAGAT ATTGCAGTAC GCTAAACAGC AGATTTTGCA GCAAACCAAG 
CAGCAGCAGC TGCAGCAGGA GCAACATACT CGCAGCAATG GCGCCGCCAG CATCAACAAT 
CGCTTCAAAT CGAACGCTCT GAAGCAGGAA CGCAGCAGCG GCTCATTGCG GAAAAGGCAA 
GAGGCATCAC GAACAGCAAC AATAGCAGCG GCAGCAGCAG CTATTGCTCA GATAACACCG 
GCAACAGTTG CAGCGGCAGC GACGCCACCA GCAGCATCGG CAAAGGCATT CAATTTGAAT 
GATCTGCAGC AACAGACGCG CCCGGCGATA TCCATGCCCA AGTCAAAGAG CTACGGAGAT 
GAGGACGATG GTGGTTCCGG TGGTGTGGCT GATCCTGGTC AGAAAACCAA GCCTGTAGGC 
TCCTCTGAGG CTGCAGGCAA ACAGCAGGCG TCGGGCAAAC AGGCCAACTC CGGCAGCGAT 
CTTGGCGCAC CTGGGAATAA AAATGCGGGC TGGTTTGGCG GACTCTGGAA CAAGTTGTCG 
CTAAAGCCGA AGAACCAAAT GATCCTGCCG GATGACAAGA ATCCGGCCAT CGTCTGGGAC 
AAGGAGCGCA AATGCTGGAC AAACACCGAA GGCAACGGCG ACGAGGCTGA AAGCTTTAAG 
CCGCCACCCA AAATGAGTGA TCTGGGCATG GCTCTGGGAG GACCACCCGC TGCGCCAATC 
GGTAATGCCG GATTGGGAAT GGGTTTAATG CCTGTGGCTA ACAATGCGCC GGGCAGCCAT 
CAATCAGCAC CGTTGATGGA TCAGCAAACG CCGTCTCAGC CGCAAACGTA TGGCAGCCCC 
ATTGATTACA CGGCTGCTCC GGCACCCGAG CTGATTCCAA CGGTACCATC GCCGGCTCCA 
CTTTCTGTCC CGTCATCTGC ACCGGCGGCA TTAGCGGCGC CCAATCCCGC GGCCGTTCCC 
GGCGGACCGC AGCCCAAGCT GCAGTCGAAC ATGTTCAAGA TGCAACGCAA CCGCACGCTA 
AAGAACTCCT ACGTGGATGT GTTCAATCCT TCGGGTGCGC CAATGTCGGC GGCACCGGAG 
AATGTGCTGG CCCCCATGAT GGCGCCGGCT GCGGTGCCCC AAGGCGGTTA CTTTGTACCC 
CAGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec16 ( NM_001298228.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285157.1 Drosophila melanogaster secretory 16, transcript variant H (Sec16), mRNA.

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CloneID	OFa21445
Clone ID Related Accession (Same CDS sequence)	NM_001298228.1
Accession Version	NM_001298228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 16, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGA GCTGCTGCCG ATGCGGCACC TTCGACTTAT 
GGAGGAGTGG CTGGAACCGC TGGACCGGTG ACCAACACTG CGGTGGCTCG TCCGCCGCGC 
AGACGCACTC CCTTGCAGTT CAGCCGACCG CATTTGGTGG CCTCGTATGC GATGAGTCTG 
CTGCTGAAGG TGAAGCCCAA ATACGCGGGA CGTGGACGAC TGCGCAACGA TGTGGAGGTG 
GCACCACCAC GCATACGGGA CGGTACGAGC AGCCTGCTGC GCATGTATCC TGGTCCCTTG 
CAGGGACGCA AGTTGCACAA GGACAAGATC ATCAGTTTCT GCAAGGAGCA GATCCGACTG 
GGGCCCACAA AGGGTTGCAC CGCACTGTAT GCCACGCAGA AGAAGCCACA GGGCAGCGTG 
ACGAAATACC GTGCCTCCCA TGCGCTCATG TGGCACCTGC TAATACTTTT GCTGCGCCAA 
AATGGGTACA TTGCGGACAC GGATGTGGGC GATCTGCTGC TGGAGAACCA GCAAGAGTAT 
CCCTATGATC CCAGCGAGTT CGAAGCAGAG AACGAGCCAG ATGCGGATGC CGAACAGGAT 
GCCGCTGCAC CGGCGGACAA GACAGTCGAT TCGGATTTAG ATAGCGAATC GGCAGCAGGA 
GTTACACCTG CGGAACCACT TGCAGCAGGC GCGGCAACTA GCTCAATCAA TGGAGCTGAT 
GCTGCAAACG CAGCAACGCC TCTGTCGGAA CAGGCGGCAA CGGACAAGTT CCGCAGCTAT 
GTGCTGCGCG GAAATGTCGA GGAGGCTCTC CAGTGGGCAA CCGACAACGG CCTGTGGACG 
CATGCATTCT TCTTGGCCCT CTACGAGGAT CGCTATGCTC TCACTGACGT AGCCCAGAAG 
TTCCTCAATC GAGCGATCAA GGCCAACGAT CCATTGCAGA CACTATACCA AATGAAGAGC 
TGCCACACAC CGGCGTGCGT CAGCCAGCTG AGGGATGAAC AGTGGGGTGA CTGGCGATCG 
CATCTCTCCA TCCTGGTGAC GAACAAGAGT CGCCAGCCGG AGTATGATCG CAGTTCGGTA 
GTGGCCCTTG GCGATACGCT TTTCCAGCGC GGCGACATTT ATGCGGCACA CTTTTGCTAT 
CTGGTGGCTC AGGAGGAGTT CGGACGATAC GATAGCTCTG CTACAGAGCT AACTACTCTG 
ACCGCCAATG TGCCCAGGCT TATTCTACTT GGCTCCTCGC ACTACAAGCA CTTCAACGAA 
TTTGCCAGCA ACGAGGCGAT CATCATGACC GAGATCTATG AGTATGCTCG ATCGCTGTTC 
GATCCGAAGT TTAGCATTGC CCACTTCCAA CACTACAAGT TCCTGCTAGC CACAAGGATC 
CTAGATTATG GTCAGCATTT CCGCTGCACC AATTATTTGG AACAAATCGC CAGGCACATC 
GAACTGAAGC CGGAAAGCTA CGACAGTAAT TTCATTCAGC GGGTTTGCGG TTTGGCCGAA 
CGCCTGCGCT ACCATGATCC CATTCTCATT AATCGTGTCT CATTTGCGAG TCCTCCGAAT 
GCCACTAGCA AGGATAGTGC TGCTGCTGAA GAGAAGGCCT GGTTGCGTCA GCTTAGATCT 
CTGGCCTATG TGCCCCAACA AGAGCAGCTG CAACAACTGC AGCAGAATCA GCAAGTCCAG 
CAGGAGCAAA ACGACATCGA TCAGCAGTTT GCAGAGGTGA ACAAGCAATT CAGGGAGCTA 
AACATGCAAT ACGAGAGCAA CAGCAACATG GAGAATACGC TGCAGCCGCA GTTGCCACCG 
GTGGATCAAC AGCCGCCGCA ACAGCAGCAA CAAGTTTTAT CGGAATCGTA CCAACAGCCA 
ACACAACAAT ATTATGAGCC ACCACCTCAA GCGCCAACCG AATCCGATCC CTATGGACAG 
GGCCAACCGT CCTATTATGA TCCCAATGCG GGGCAGCTTC ATTACGATCC AAATGCTGCA 
CAGCATCAAT ACGACCAGCA GCAACCTCAA CCACAGCCGA CTCCCAGCTA TGGCCAAATA 
GAGCCAGGAA CTGAGGCCTA CCAACCTGGA CAAGCAACAG CAGATCCAGC TGCGGTTCCC 
TCTGGCTACG GATACGATTA CTGGTCGGGC ACACAGCAGC CACCTTATGG TGACGAGCCC 
AATGAGAATC AAGCTGCACT AAAGGATGAA GAGGAGGAGG ATGAGGAGCA GGATGAACAG 
CAGATATTGC AGTACGCTAA ACAGCAGATT TTGCAGCAAA CCAAGCAGCA GCAGCTGCAG 
CAGGAGCAAC ATACTCGCAG CAATGGCGCC GCCAGCATCA ACAATCGCTT CAAATCGAAC 
GCTCTGAAGC AGGAACGCAG CAGCGGCTCA TTGCGGAAAA GGCAAGAGGC ATCACGAACA 
GCAACAATAG CAGCGGCAGC AGCAGCTATT GCTCAGATAA CACCGGCAAC AGTTGCAGCG 
GCAGCGACGC CACCAGCAGC ATCGGCAAAG GCATTCAATT TGAATGATCT GCAGCAACAG 
ACGCGCCCGG CGATATCCAT GCCCAAGTCA AAGAGCTACG GAGATGAGGA CGATGGTGGT 
TCCGGTGGTG TGGCTGATCC TGGTCAGAAA ACCAAGCCTG TAGGCTCCTC TGAGGCTGCA 
GGCAAACAGC AGGCGTCGGG CAAACAGGCC AACTCCGGCA GCGATCTTGG CGCACCTGGG 
AATAAAAATG CGGGCTGGTT TGGCGGACTC TGGAACAAGT TGTCGCTAAA GCCGAAGAAC 
CAAATGATCC TGCCGGATGA CAAGAATCCG GCCATCGTCT GGGACAAGGA GCGCAAATGC 
TGGACAAACA CCGAAGGCAA CGGCGACGAG GCTGAAAGCT TTAAGCCGCC ACCCAAAATG 
AGTGATCTGG GCATGGCTCT GGGAGGACCA CCCGCTGCGC CAATCGGTAA TGCCGGATTG 
GGAATGGGTT TAATGCCTGT GGCTAACAAT GCGCCGGGCA GCCATCAATC AGCACCGTTG 
ATGGATCAGC AAACGCCGTC TCAGCCGCAA ACGTATGGCA GCCCCATTGA TTACACGGCT 
GCTCCGGCAC CCGAGCTGAT TCCAACGGTA CCATCGCCGG CTCCACTTTC TGTCCCGTCA 
TCTGCACCGG CGGCATTAGC GGCGCCCAAT CCCGCGGCCG TTCCCGGCGG ACCGCAGCCC 
AAGCTGCAGT CGAACATGTT CAAGATGCAA CGCAACCGCA CGCTAAAGAA CTCCTACGTG 
GATGTGTTCA ATCCTTCGGG TGCGCCAATG TCGGCGGCAC CGGAGAATGT GCTGGCCCCC 
ATGATGGCGC CGGCTGCGGT GCCCCAAGGC GGTTACTTTG TACCCCAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285157.1
CDS	196..6609
Translation	MLHNNAPWLPPHQQQQQQAPQQHPTPQQQHAMPQQQQTLPAQQQQQQVYASQMFQQHQPNYWPEDQHQQQQQPQSLNYNNYFPGQQQQHPMQQQQLPPQQQLPPQQQQPTQQQHTIQQQLYYPTQQQPQVPAPAEPALDSFDNNNSGGGGGGGRSDGWGDWGDWNDNSNNNNSNGTDGLLEPTGQLLEDSFNVQSSQGSWQAFATSNNVNNNGELPPPANPQSTSLQQQPLNQQSIPLLHQQPQSPPELGQEPELDAIVPPQAFQNQPPTAAPPPTSLTSFSAQMTAVGSPIYAVGASSAHSAGVGASPAPPTGVVAPPAHLAGIGAPPAHLAGVGASPAPSALVGASPAPIIGVGAPPVGAPPVGAPPADATTIAPPAALPPSIVPSSGSNPFKRSTGLNKRVNIMADPAAGAPSPPAPAAAIAPVAPVAPIPPPADQLFGMPAEAHGEGFNLIAAPPVEASLGTPLSAPIPAPIPVPNASLYASPAVPQAFAHLEPDNQEVLSAPNDERAQYLQTSHLSEQLGEGEADQDAGLLPPPGLSRLVLGQPELDSQQQRQVTGATEQPPLNVAQAAALHMQERRADGEDTSDGEQQVRNIQTPPRRVVTGVETNAPSLREQREVVLDGENLEDREAIPPPTLAELPTTSVHHNILPDEAEQLHHNNPPQAMTPLNAQQPHQQQSTLQQQHQQHSTTQQEKKRAAVGRRTTASLDLESDESDEFLQSERERDRERERRDLMEERHSRGRSHSHYPYEGETEDSVRGAAHHETKSLRETHHKRNHDSARSRRHQDPKVERERERDRERDRTWRRRSNKYHSGGEDPDRSYDHSRRYNNSNYEGESDNPEYNHMGDAELDGSGGRSSKTSRHRRSAAEEDFDDYERERNRSRRSTKPQSSAEKSRSSGGRRNYENSGRSARADDGRRRYQDQRNPGAQYPVSATGYVPYGMYEQMSRNPQVYADMYAKFYGQMINSMNAAVSAAASKGGVPAGAGVIPGLMPSAVPVSAAQLVAATSGGSVSGSSEAAMLRERERAAADAAPSTYGGVAGTAGPVTNTAVARPPRRRTPLQFSRPHLVASYAMSLLLKVKPKYAGRGRLRNDVEVAPPRIRDGTSSLLRMYPGPLQGRKLHKDKIISFCKEQIRLGPTKGCTALYATQKKPQGSVTKYRASHALMWHLLILLLRQNGYIADTDVGDLLLENQQEYPYDPSEFEAENEPDADAEQDAAAPADKTVDSDLDSESAAGVTPAEPLAAGAATSSINGADAANAATPLSEQAATDKFRSYVLRGNVEEALQWATDNGLWTHAFFLALYEDRYALTDVAQKFLNRAIKANDPLQTLYQMKSCHTPACVSQLRDEQWGDWRSHLSILVTNKSRQPEYDRSSVVALGDTLFQRGDIYAAHFCYLVAQEEFGRYDSSATELTTLTANVPRLILLGSSHYKHFNEFASNEAIIMTEIYEYARSLFDPKFSIAHFQHYKFLLATRILDYGQHFRCTNYLEQIARHIELKPESYDSNFIQRVCGLAERLRYHDPILINRVSFASPPNATSKDSAAAEEKAWLRQLRSLAYVPQQEQLQQLQQNQQVQQEQNDIDQQFAEVNKQFRELNMQYESNSNMENTLQPQLPPVDQQPPQQQQQVLSESYQQPTQQYYEPPPQAPTESDPYGQGQPSYYDPNAGQLHYDPNAAQHQYDQQQPQPQPTPSYGQIEPGTEAYQPGQATADPAAVPSGYGYDYWSGTQQPPYGDEPNENQAALKDEEEEDEEQDEQQILQYAKQQILQQTKQQQLQQEQHTRSNGAASINNRFKSNALKQERSSGSLRKRQEASRTATIAAAAAAIAQITPATVAAAATPPAASAKAFNLNDLQQQTRPAISMPKSKSYGDEDDGGSGGVADPGQKTKPVGSSEAAGKQQASGKQANSGSDLGAPGNKNAGWFGGLWNKLSLKPKNQMILPDDKNPAIVWDKERKCWTNTEGNGDEAESFKPPPKMSDLGMALGGPPAAPIGNAGLGMGLMPVANNAPGSHQSAPLMDQQTPSQPQTYGSPIDYTAAPAPELIPTVPSPAPLSVPSSAPAALAAPNPAAVPGGPQPKLQSNMFKMQRNRTLKNSYVDVFNPSGAPMSAAPENVLAPMMAPAAVPQGGYFVPQQ

Target ORF information:

RefSeq Version	NM_001298228.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 16, transcript variant H (Sec16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298228.1

ORF Insert Sequence:

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGA GCTGCTGCCG ATGCGGCACC TTCGACTTAT 
GGAGGAGTGG CTGGAACCGC TGGACCGGTG ACCAACACTG CGGTGGCTCG TCCGCCGCGC 
AGACGCACTC CCTTGCAGTT CAGCCGACCG CATTTGGTGG CCTCGTATGC GATGAGTCTG 
CTGCTGAAGG TGAAGCCCAA ATACGCGGGA CGTGGACGAC TGCGCAACGA TGTGGAGGTG 
GCACCACCAC GCATACGGGA CGGTACGAGC AGCCTGCTGC GCATGTATCC TGGTCCCTTG 
CAGGGACGCA AGTTGCACAA GGACAAGATC ATCAGTTTCT GCAAGGAGCA GATCCGACTG 
GGGCCCACAA AGGGTTGCAC CGCACTGTAT GCCACGCAGA AGAAGCCACA GGGCAGCGTG 
ACGAAATACC GTGCCTCCCA TGCGCTCATG TGGCACCTGC TAATACTTTT GCTGCGCCAA 
AATGGGTACA TTGCGGACAC GGATGTGGGC GATCTGCTGC TGGAGAACCA GCAAGAGTAT 
CCCTATGATC CCAGCGAGTT CGAAGCAGAG AACGAGCCAG ATGCGGATGC CGAACAGGAT 
GCCGCTGCAC CGGCGGACAA GACAGTCGAT TCGGATTTAG ATAGCGAATC GGCAGCAGGA 
GTTACACCTG CGGAACCACT TGCAGCAGGC GCGGCAACTA GCTCAATCAA TGGAGCTGAT 
GCTGCAAACG CAGCAACGCC TCTGTCGGAA CAGGCGGCAA CGGACAAGTT CCGCAGCTAT 
GTGCTGCGCG GAAATGTCGA GGAGGCTCTC CAGTGGGCAA CCGACAACGG CCTGTGGACG 
CATGCATTCT TCTTGGCCCT CTACGAGGAT CGCTATGCTC TCACTGACGT AGCCCAGAAG 
TTCCTCAATC GAGCGATCAA GGCCAACGAT CCATTGCAGA CACTATACCA AATGAAGAGC 
TGCCACACAC CGGCGTGCGT CAGCCAGCTG AGGGATGAAC AGTGGGGTGA CTGGCGATCG 
CATCTCTCCA TCCTGGTGAC GAACAAGAGT CGCCAGCCGG AGTATGATCG CAGTTCGGTA 
GTGGCCCTTG GCGATACGCT TTTCCAGCGC GGCGACATTT ATGCGGCACA CTTTTGCTAT 
CTGGTGGCTC AGGAGGAGTT CGGACGATAC GATAGCTCTG CTACAGAGCT AACTACTCTG 
ACCGCCAATG TGCCCAGGCT TATTCTACTT GGCTCCTCGC ACTACAAGCA CTTCAACGAA 
TTTGCCAGCA ACGAGGCGAT CATCATGACC GAGATCTATG AGTATGCTCG ATCGCTGTTC 
GATCCGAAGT TTAGCATTGC CCACTTCCAA CACTACAAGT TCCTGCTAGC CACAAGGATC 
CTAGATTATG GTCAGCATTT CCGCTGCACC AATTATTTGG AACAAATCGC CAGGCACATC 
GAACTGAAGC CGGAAAGCTA CGACAGTAAT TTCATTCAGC GGGTTTGCGG TTTGGCCGAA 
CGCCTGCGCT ACCATGATCC CATTCTCATT AATCGTGTCT CATTTGCGAG TCCTCCGAAT 
GCCACTAGCA AGGATAGTGC TGCTGCTGAA GAGAAGGCCT GGTTGCGTCA GCTTAGATCT 
CTGGCCTATG TGCCCCAACA AGAGCAGCTG CAACAACTGC AGCAGAATCA GCAAGTCCAG 
CAGGAGCAAA ACGACATCGA TCAGCAGTTT GCAGAGGTGA ACAAGCAATT CAGGGAGCTA 
AACATGCAAT ACGAGAGCAA CAGCAACATG GAGAATACGC TGCAGCCGCA GTTGCCACCG 
GTGGATCAAC AGCCGCCGCA ACAGCAGCAA CAAGTTTTAT CGGAATCGTA CCAACAGCCA 
ACACAACAAT ATTATGAGCC ACCACCTCAA GCGCCAACCG AATCCGATCC CTATGGACAG 
GGCCAACCGT CCTATTATGA TCCCAATGCG GGGCAGCTTC ATTACGATCC AAATGCTGCA 
CAGCATCAAT ACGACCAGCA GCAACCTCAA CCACAGCCGA CTCCCAGCTA TGGCCAAATA 
GAGCCAGGAA CTGAGGCCTA CCAACCTGGA CAAGCAACAG CAGATCCAGC TGCGGTTCCC 
TCTGGCTACG GATACGATTA CTGGTCGGGC ACACAGCAGC CACCTTATGG TGACGAGCCC 
AATGAGAATC AAGCTGCACT AAAGGATGAA GAGGAGGAGG ATGAGGAGCA GGATGAACAG 
CAGATATTGC AGTACGCTAA ACAGCAGATT TTGCAGCAAA CCAAGCAGCA GCAGCTGCAG 
CAGGAGCAAC ATACTCGCAG CAATGGCGCC GCCAGCATCA ACAATCGCTT CAAATCGAAC 
GCTCTGAAGC AGGAACGCAG CAGCGGCTCA TTGCGGAAAA GGCAAGAGGC ATCACGAACA 
GCAACAATAG CAGCGGCAGC AGCAGCTATT GCTCAGATAA CACCGGCAAC AGTTGCAGCG 
GCAGCGACGC CACCAGCAGC ATCGGCAAAG GCATTCAATT TGAATGATCT GCAGCAACAG 
ACGCGCCCGG CGATATCCAT GCCCAAGTCA AAGAGCTACG GAGATGAGGA CGATGGTGGT 
TCCGGTGGTG TGGCTGATCC TGGTCAGAAA ACCAAGCCTG TAGGCTCCTC TGAGGCTGCA 
GGCAAACAGC AGGCGTCGGG CAAACAGGCC AACTCCGGCA GCGATCTTGG CGCACCTGGG 
AATAAAAATG CGGGCTGGTT TGGCGGACTC TGGAACAAGT TGTCGCTAAA GCCGAAGAAC 
CAAATGATCC TGCCGGATGA CAAGAATCCG GCCATCGTCT GGGACAAGGA GCGCAAATGC 
TGGACAAACA CCGAAGGCAA CGGCGACGAG GCTGAAAGCT TTAAGCCGCC ACCCAAAATG 
AGTGATCTGG GCATGGCTCT GGGAGGACCA CCCGCTGCGC CAATCGGTAA TGCCGGATTG 
GGAATGGGTT TAATGCCTGT GGCTAACAAT GCGCCGGGCA GCCATCAATC AGCACCGTTG 
ATGGATCAGC AAACGCCGTC TCAGCCGCAA ACGTATGGCA GCCCCATTGA TTACACGGCT 
GCTCCGGCAC CCGAGCTGAT TCCAACGGTA CCATCGCCGG CTCCACTTTC TGTCCCGTCA 
TCTGCACCGG CGGCATTAGC GGCGCCCAAT CCCGCGGCCG TTCCCGGCGG ACCGCAGCCC 
AAGCTGCAGT CGAACATGTT CAAGATGCAA CGCAACCGCA CGCTAAAGAA CTCCTACGTG 
GATGTGTTCA ATCCTTCGGG TGCGCCAATG TCGGCGGCAC CGGAGAATGT GCTGGCCCCC 
ATGATGGCGC CGGCTGCGGT GCCCCAAGGC GGTTACTTTG TACCCCAGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sec16 ( NM_001298229.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285158.1 Drosophila melanogaster secretory 16, transcript variant I (Sec16), mRNA.

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CloneID	OFa21446
Clone ID Related Accession (Same CDS sequence)	NM_001298229.1
Accession Version	NM_001298229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	secretory 16, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGG TATACACACG CATACATAAC CCAAGCCAAT 
GAATTCCATC GTCACCAATA CAAAGAGCTG ATCTACCAGC AACAACAACA ACAACAGCAG 
CAGCAACAAC AACAACAGCA GAGGGAGGAT CACCTGAACT CCAGCTTTAG CGTTGCAGAG 
GATAATGCCA GTTTTTACGG ATCGCACGGA GGAGGTTCCA TCTATAACCA ATACCAATCG 
TATCCACTAT CCAGTGCCCG ATCGTTGAGT AACTTGAACG GCGATGGACG CAATTCCCGA 
TGCGGACCGT ATTACGCTGG TTCCGAATGC GGTCTCGATA TCAGAGCTGC TGCCGATGCG 
GCACCTTCGA CTTATGGAGG AGTGGCTGGA ACCGCTGGAC CGGTGACCAA CACTGCGGTG 
GCTCGTCCGC CGCGCAGACG CACTCCCTTG CAGTTCAGCC GACCGCATTT GGTGGCCTCG 
TATGCGATGA GTCTGCTGCT GAAGGTGAAG CCCAAATACG CGGGACGTGG ACGACTGCGC 
AACGATGTGG AGGTGGCACC ACCACGCATA CGGGACGGTA CGAGCAGCCT GCTGCGCATG 
TATCCTGGTC CCTTGCAGGG ACGCAAGTTG CACAAGGACA AGATCATCAG TTTCTGCAAG 
GAGCAGATCC GACTGGGGCC CACAAAGGGT TGCACCGCAC TGTATGCCAC GCAGAAGAAG 
CCACAGGGCA GCGTGACGAA ATACCGTGCC TCCCATGCGC TCATGTGGCA CCTGCTAATA 
CTTTTGCTGC GCCAAAATGG GTACATTGCG GACACGGATG TGGGCGATCT GCTGCTGGAG 
AACCAGCAAG AGTATCCCTA TGATCCCAGC GAGTTCGAAG CAGAGAACGA GCCAGATGCG 
GATGCCGAAC AGGATGCCGC TGCACCGGCG GACAAGACAG TCGATTCGGA TTTAGATAGC 
GAATCGGCAG CAGGAGTTAC ACCTGCGGAA CCACTTGCAG CAGGCGCGGC AACTAGCTCA 
ATCAATGGAG CTGATGCTGC AAACGCAGCA ACGCCTCTGT CGGAACAGGC GGCAACGGAC 
AAGTTCCGCA GCTATGTGCT GCGCGGAAAT GTCGAGGAGG CTCTCCAGTG GGCAACCGAC 
AACGGCCTGT GGACGCATGC ATTCTTCTTG GCCCTCTACG AGGATCGCTA TGCTCTCACT 
GACGTAGCCC AGAAGTTCCT CAATCGAGCG ATCAAGGCCA ACGATCCATT GCAGACACTA 
TACCAAATGA AGAGCTGCCA CACACCGGCG TGCGTCAGCC AGCTGAGGGA TGAACAGTGG 
GGTGACTGGC GATCGCATCT CTCCATCCTG GTGACGAACA AGAGTCGCCA GCCGGAGTAT 
GATCGCAGTT CGGTAGTGGC CCTTGGCGAT ACGCTTTTCC AGCGCGGCGA CATTTATGCG 
GCACACTTTT GCTATCTGGT GGCTCAGGAG GAGTTCGGAC GATACGATAG CTCTGCTACA 
GAGCTAACTA CTCTGACCGC CAATGTGCCC AGGCTTATTC TACTTGGCTC CTCGCACTAC 
AAGCACTTCA ACGAATTTGC CAGCAACGAG GCGATCATCA TGACCGAGAT CTATGAGTAT 
GCTCGATCGC TGTTCGATCC GAAGTTTAGC ATTGCCCACT TCCAACACTA CAAGTTCCTG 
CTAGCCACAA GGATCCTAGA TTATGGTCAG CATTTCCGCT GCACCAATTA TTTGGAACAA 
ATCGCCAGGC ACATCGAACT GAAGCCGGAA AGCTACGACA GTAATTTCAT TCAGCGGGTT 
TGCGGTTTGG CCGAACGCCT GCGCTACCAT GATCCCATTC TCATTAATCG TGTCTCATTT 
GCGAGTCCTC CGAATGCCAC TAGCAAGGAT AGTGCTGCTG CTGAAGAGAA GGCCTGGTTG 
CGTCAGCTTA GATCTCTGGC CTATGTGCAG CCCCAACAAG AGCAGCTGCA ACAACTGCAG 
CAGAATCAGC AAGTCCAGCA GGAGCAAAAC GACATCGATC AGCAGTTTGC AGAGGTGAAC 
AAGCAATTCA GGGAGCTAAA CATGCAATAC GAGAGCAACA GCAACATGGA GAATACGCTG 
CAGCCGCAGT TGCCACCGGT GGATCAACAG CCGCCGCAAC AGCAGCAACA AGTTTTATCG 
GAATCGTACC AACAGCCAAC ACAACAATAT TATGAGCCAC CACCTCAAGC GCCAACCGAA 
TCCGATCCCT ATGGACAGGG CCAACCGTCC TATTATGATC CCAATGCGGG GCAGCTTCAT 
TACGATCCAA ATGCTGCACA GCATCAATAC GACCAGCAGC AACCTCAACC ACAGCCGACT 
CCCAGCTATG GCCAAATAGA GCCAGGAACT GAGGCCTACC AACCTGGACA AGCAACAGCA 
GATCCAGCTG CGGTTCCCTC TGGCTACGGA TACGATTACT GGTCGGGCAC ACAGCAGCCA 
CCTTATGGTG ACGAGCCCAA TGAGAATCAA GCTGCACTAA AGGATGAAGA GGAGGAGGAT 
GAGGAGCAGG ATGAACAGCA GATATTGCAG TACGCTAAAC AGCAGATTTT GCAGCAAACC 
AAGCAGCAGC AGCTGCAGCA GGAGCAACAT ACTCGCAGCA ATGGCGCCGC CAGCATCAAC 
AATCGCTTCA AATCGAACGC TCTGAAGCAG GAACGCAGCA GCGGCTCATT GCGGAAAAGG 
CAAGAGGCAT CACGAACAGC AACAATAGCA GCGGCAGCAG CAGCTATTGC TCAGATAACA 
CCGGCAACAG TTGCAGCGGC AGCGACGCCA CCAGCAGCAT CGGCAAAGGC ATTCAATTTG 
AATGATGTAA GTAGCTTTTC CAGCACAAAT AAACGACATC AAGCTACCAG CAGCAGCAAC 
AATCGCAGCA GCAGCAACCA AGCAGCAGCA GCAGCAGCAG CAACAAAAGC TGCAGCAATA 
GCAACAGTAC CTGCTGCCCC TAATCAATTG CTAAGTTGTA GAAAGTCTAA CAGCCAGAAT 
AGTCCATACT TAGCCACGCC AAAAACGCTT ACCACGCCCA CACGTTTTAT TGCCGCTAAA 
GCAGCCACTG TTACAGCCAC GCCCACAGCG CCAGCCACTT CGACGACAAC GTCGACAACC 
ATAGGTGCTA GAGGATCATC TACCAAACGC GTTTACTTTA ATCTTCCGTC GACCATTGCT 
GAATCGGAGC AAGAGTTCGA ACATGAGCTG GATTATGATG AGGAGATGGA GGAGGAGAAC 
AGTATGGCTC TGGCGCAAGC TAAACCAAAC GTTCATCTAG CGCGAAAGCC ACATATTGTC 
TTTAATGGAA CGTATCCCAT TGATATGCCA CTGACCCAGG GGCAGAGTCA ACTCAAAAGC 
AACTCGTATG ATCCGGAGGC CAATGCAGAT CGTGGGGATC TACAGCGCTA TAGGAACTAC 
ATGCTCCACG ATTATGTTAT AGATGAGGAT GCCGAGGGCG AGGAGCAGGC CGAAGAAGAG 
GGCGAGGAGG ATGAGTATGG AGATGGTGAT GAGGATTTAT TCCCCTATGC CGACGACGAT 
GAGAGCTTCG ATGATGGCGA TTACTATACG CATCAACTGC TCAATAAGTA TGGGATTATA 
CCAAGCGGTG AGAAACAGAG AGTCGAGAGA ACACCGGCAT CCGTGGAGGC AGCTACCTCC 
GCGGCCCAGC CACAGCACGT GTGGTCCATG AAGGAGCTGA TAGCCAATCC AGCCATACCA 
CTTAACTACA AGAAAATGTG CTCCGAAGTG GAGCAGAGCC TCAGTCGATT CGAGAATTAT 
TTGGACAGCA AAAAGGAGCA ACAGGTGCCA CCGGGAGCCG CAGTGCGCAC CTTCGACATC 
GATGCACCCA CTCGAAGAGG CCACCGAAGC AGCACATCCA CAAATGCTGG CTTGGATCTT 
AGATCTAAGA AGAAATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285158.1
CDS	196..7176
Translation	MLHNNAPWLPPHQQQQQQAPQQHPTPQQQHAMPQQQQTLPAQQQQQQVYASQMFQQHQPNYWPEDQHQQQQQPQSLNYNNYFPGQQQQHPMQQQQLPPQQQLPPQQQQPTQQQHTIQQQLYYPTQQQPQVPAPAEPALDSFDNNNSGGGGGGGRSDGWGDWGDWNDNSNNNNSNGTDGLLEPTGQLLEDSFNVQSSQGSWQAFATSNNVNNNGELPPPANPQSTSLQQQPLNQQSIPLLHQQPQSPPELGQEPELDAIVPPQAFQNQPPTAAPPPTSLTSFSAQMTAVGSPIYAVGASSAHSAGVGASPAPPTGVVAPPAHLAGIGAPPAHLAGVGASPAPSALVGASPAPIIGVGAPPVGAPPVGAPPADATTIAPPAALPPSIVPSSGSNPFKRSTGLNKRVNIMADPAAGAPSPPAPAAAIAPVAPVAPIPPPADQLFGMPAEAHGEGFNLIAAPPVEASLGTPLSAPIPAPIPVPNASLYASPAVPQAFAHLEPDNQEVLSAPNDERAQYLQTSHLSEQLGEGEADQDAGLLPPPGLSRLVLGQPELDSQQQRQVTGATEQPPLNVAQAAALHMQERRADGEDTSDGEQQVRNIQTPPRRVVTGVETNAPSLREQREVVLDGENLEDREAIPPPTLAELPTTSVHHNILPDEAEQLHHNNPPQAMTPLNAQQPHQQQSTLQQQHQQHSTTQQEKKRAAVGRRTTASLDLESDESDEFLQSERERDRERERRDLMEERHSRGRSHSHYPYEGETEDSVRGAAHHETKSLRETHHKRNHDSARSRRHQDPKVERERERDRERDRTWRRRSNKYHSGGEDPDRSYDHSRRYNNSNYEGESDNPEYNHMGDAELDGSGGRSSKTSRHRRSAAEEDFDDYERERNRSRRSTKPQSSAEKSRSSGGRRNYENSGRSARADDGRRRYQDQRNPGAQYPVSATGYVPYGMYEQMSRNPQVYADMYAKFYGQMINSMNAAVSAAASKGGVPAGAGVIPGLMPSAVPVSAAQLVAATSGGSVSGSSEAAMLRERERYTHAYITQANEFHRHQYKELIYQQQQQQQQQQQQQQQREDHLNSSFSVAEDNASFYGSHGGGSIYNQYQSYPLSSARSLSNLNGDGRNSRCGPYYAGSECGLDIRAAADAAPSTYGGVAGTAGPVTNTAVARPPRRRTPLQFSRPHLVASYAMSLLLKVKPKYAGRGRLRNDVEVAPPRIRDGTSSLLRMYPGPLQGRKLHKDKIISFCKEQIRLGPTKGCTALYATQKKPQGSVTKYRASHALMWHLLILLLRQNGYIADTDVGDLLLENQQEYPYDPSEFEAENEPDADAEQDAAAPADKTVDSDLDSESAAGVTPAEPLAAGAATSSINGADAANAATPLSEQAATDKFRSYVLRGNVEEALQWATDNGLWTHAFFLALYEDRYALTDVAQKFLNRAIKANDPLQTLYQMKSCHTPACVSQLRDEQWGDWRSHLSILVTNKSRQPEYDRSSVVALGDTLFQRGDIYAAHFCYLVAQEEFGRYDSSATELTTLTANVPRLILLGSSHYKHFNEFASNEAIIMTEIYEYARSLFDPKFSIAHFQHYKFLLATRILDYGQHFRCTNYLEQIARHIELKPESYDSNFIQRVCGLAERLRYHDPILINRVSFASPPNATSKDSAAAEEKAWLRQLRSLAYVQPQQEQLQQLQQNQQVQQEQNDIDQQFAEVNKQFRELNMQYESNSNMENTLQPQLPPVDQQPPQQQQQVLSESYQQPTQQYYEPPPQAPTESDPYGQGQPSYYDPNAGQLHYDPNAAQHQYDQQQPQPQPTPSYGQIEPGTEAYQPGQATADPAAVPSGYGYDYWSGTQQPPYGDEPNENQAALKDEEEEDEEQDEQQILQYAKQQILQQTKQQQLQQEQHTRSNGAASINNRFKSNALKQERSSGSLRKRQEASRTATIAAAAAAIAQITPATVAAAATPPAASAKAFNLNDVSSFSSTNKRHQATSSSNNRSSSNQAAAAAAATKAAAIATVPAAPNQLLSCRKSNSQNSPYLATPKTLTTPTRFIAAKAATVTATPTAPATSTTTSTTIGARGSSTKRVYFNLPSTIAESEQEFEHELDYDEEMEEENSMALAQAKPNVHLARKPHIVFNGTYPIDMPLTQGQSQLKSNSYDPEANADRGDLQRYRNYMLHDYVIDEDAEGEEQAEEEGEEDEYGDGDEDLFPYADDDESFDDGDYYTHQLLNKYGIIPSGEKQRVERTPASVEAATSAAQPQHVWSMKELIANPAIPLNYKKMCSEVEQSLSRFENYLDSKKEQQVPPGAAVRTFDIDAPTRRGHRSSTSTNAGLDLRSKKKL

Target ORF information:

RefSeq Version	NM_001298229.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster secretory 16, transcript variant I (Sec16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298229.1

ORF Insert Sequence:

ATGTTGCACA ATAATGCGCC CTGGCTGCCA CCGCATCAGC AGCAGCAGCA ACAGGCGCCG 
CAGCAACATC CTACTCCACA GCAGCAACAT GCAATGCCCC AACAGCAGCA AACTCTTCCT 
GCCCAGCAGC AGCAGCAGCA AGTCTATGCC AGCCAAATGT TCCAGCAACA TCAGCCAAAT 
TATTGGCCAG AGGATCAGCA TCAACAGCAG CAGCAGCCAC AATCCCTGAA CTACAATAAC 
TACTTTCCGG GACAGCAGCA GCAACATCCT ATGCAGCAGC AGCAACTGCC ACCGCAGCAG 
CAACTGCCAC CACAGCAGCA ACAGCCTACG CAGCAGCAAC ATACAATCCA GCAGCAACTG 
TATTATCCCA CTCAGCAGCA GCCACAAGTC CCGGCTCCAG CGGAACCAGC TCTGGACTCG 
TTTGACAACA ATAATAGCGG AGGAGGTGGT GGTGGCGGCC GGAGCGATGG CTGGGGTGAT 
TGGGGCGATT GGAACGACAA TAGCAACAAC AATAATAGCA ACGGCACCGA TGGCTTGTTA 
GAGCCAACCG GACAACTGCT GGAGGATTCC TTCAATGTTC AATCCTCGCA AGGCAGTTGG 
CAGGCGTTTG CCACTAGCAA TAATGTCAAT AACAATGGAG AGTTGCCGCC ACCAGCCAAT 
CCGCAGTCAA CGTCCTTGCA ACAACAGCCA TTGAATCAGC AATCGATACC TTTGCTGCAC 
CAGCAGCCAC AATCGCCGCC GGAACTGGGG CAGGAACCCG AACTGGATGC CATTGTGCCT 
CCCCAGGCTT TCCAAAACCA ACCACCAACT GCAGCACCAC CACCAACATC ACTGACTTCC 
TTCTCTGCAC AAATGACTGC TGTGGGATCG CCAATCTATG CTGTTGGAGC ATCTTCAGCA 
CATAGCGCAG GAGTAGGAGC TTCACCAGCA CCTCCCACTG GAGTAGTAGC TCCGCCAGCA 
CATCTCGCCG GTATAGGAGC TCCACCAGCA CATCTTGCAG GAGTAGGAGC TTCGCCAGCA 
CCTTCCGCTT TAGTAGGAGC TTCTCCAGCA CCCATCATTG GAGTAGGAGC ACCACCAGTG 
GGAGCACCAC CAGTAGGAGC ACCGCCAGCG GACGCTACAA CTATTGCACC ACCGGCCGCA 
CTGCCACCTA GCATAGTTCC ATCGTCTGGT TCCAATCCCT TCAAGCGTTC CACGGGCCTA 
AATAAGCGAG TGAACATAAT GGCAGATCCT GCAGCAGGAG CTCCATCGCC TCCAGCTCCT 
GCTGCTGCAA TTGCTCCTGT GGCACCAGTA GCACCAATTC CGCCACCAGC AGATCAGCTC 
TTTGGTATGC CAGCAGAAGC TCATGGAGAG GGTTTCAACT TAATTGCGGC GCCACCTGTT 
GAGGCTTCAC TTGGAACTCC ACTAAGTGCA CCTATTCCTG CACCCATTCC TGTACCTAAC 
GCATCGCTCT ATGCATCACC GGCTGTTCCA CAGGCTTTTG CGCATTTGGA ACCGGACAAT 
CAGGAGGTTT TGTCCGCGCC AAATGACGAA CGGGCCCAGT ACTTGCAGAC CAGCCACCTG 
TCCGAGCAGC TGGGCGAAGG TGAAGCGGAT CAGGATGCGG GCCTGCTGCC ACCACCAGGA 
CTGTCTCGTC TGGTTTTGGG CCAACCGGAG TTGGATTCGC AGCAGCAACG CCAGGTCACT 
GGAGCCACAG AGCAGCCACC ACTTAATGTG GCCCAGGCAG CTGCTCTGCA TATGCAAGAA 
CGTCGCGCAG ATGGCGAGGA CACCTCTGAC GGCGAGCAGC AAGTGCGTAA TATACAGACG 
CCACCTCGTC GCGTCGTAAC CGGCGTGGAG ACCAATGCCC CGTCGTTGCG AGAACAGCGG 
GAAGTGGTGC TGGATGGCGA GAATCTGGAG GATCGCGAAG CTATACCGCC ACCAACGTTG 
GCTGAACTGC CAACAACTTC AGTACATCAC AATATTCTAC CCGACGAGGC GGAGCAGCTG 
CATCACAACA ATCCGCCCCA GGCGATGACC CCGCTAAATG CACAGCAACC ACATCAGCAG 
CAGTCCACGC TGCAGCAGCA ACACCAGCAA CATTCAACCA CTCAGCAGGA GAAGAAGCGT 
GCAGCAGTAG GACGCCGGAC CACCGCGTCG CTGGATCTAG AGTCCGATGA GTCCGATGAA 
TTCCTGCAGA GCGAAAGGGA ACGGGATCGG GAGCGTGAGC GCAGGGATCT AATGGAGGAG 
AGGCACAGTC GTGGACGCAG CCATAGTCAC TATCCCTACG AAGGCGAAAC GGAGGACTCT 
GTGCGTGGTG CTGCCCATCA CGAGACGAAA TCCTTGAGAG AAACACATCA CAAACGTAAC 
CACGATTCAG CACGCTCTCG TCGCCATCAG GATCCTAAAG TGGAACGTGA AAGGGAGCGG 
GATAGGGAGC GTGACCGCAC ATGGCGTCGA CGATCCAACA AGTATCACAG CGGTGGCGAG 
GATCCGGATC GTTCGTACGA TCATTCGCGT CGCTATAACA ACAGCAACTA CGAGGGCGAG 
TCGGATAATC CGGAGTATAA TCACATGGGC GACGCAGAAC TTGATGGCAG CGGTGGACGG 
AGCAGTAAAA CCAGTCGCCA TCGACGCAGT GCCGCCGAGG AGGATTTCGA TGATTACGAA 
CGGGAACGTA ATCGATCCCG GCGCTCAACC AAACCACAAT CGTCGGCTGA AAAGTCACGC 
TCCTCGGGCG GACGACGGAA TTACGAGAAT TCGGGTCGAA GTGCCCGTGC GGATGATGGA 
CGCAGACGTT ATCAGGATCA GCGCAATCCT GGTGCTCAGT ATCCCGTGTC AGCCACCGGC 
TATGTTCCCT ACGGAATGTA CGAGCAAATG TCGCGGAATC CACAAGTCTA CGCTGATATG 
TATGCAAAGT TCTATGGCCA AATGATCAAC TCGATGAACG CAGCTGTTTC GGCGGCTGCT 
TCGAAAGGTG GAGTTCCCGC TGGAGCAGGA GTCATACCCG GATTGATGCC CAGCGCGGTG 
CCCGTCAGTG CCGCCCAGTT GGTGGCCGCC ACCAGCGGAG GAAGTGTCAG CGGTAGCAGT 
GAAGCCGCCA TGCTCAGGGA GCGAGAAAGG TATACACACG CATACATAAC CCAAGCCAAT 
GAATTCCATC GTCACCAATA CAAAGAGCTG ATCTACCAGC AACAACAACA ACAACAGCAG 
CAGCAACAAC AACAACAGCA GAGGGAGGAT CACCTGAACT CCAGCTTTAG CGTTGCAGAG 
GATAATGCCA GTTTTTACGG ATCGCACGGA GGAGGTTCCA TCTATAACCA ATACCAATCG 
TATCCACTAT CCAGTGCCCG ATCGTTGAGT AACTTGAACG GCGATGGACG CAATTCCCGA 
TGCGGACCGT ATTACGCTGG TTCCGAATGC GGTCTCGATA TCAGAGCTGC TGCCGATGCG 
GCACCTTCGA CTTATGGAGG AGTGGCTGGA ACCGCTGGAC CGGTGACCAA CACTGCGGTG 
GCTCGTCCGC CGCGCAGACG CACTCCCTTG CAGTTCAGCC GACCGCATTT GGTGGCCTCG 
TATGCGATGA GTCTGCTGCT GAAGGTGAAG CCCAAATACG CGGGACGTGG ACGACTGCGC 
AACGATGTGG AGGTGGCACC ACCACGCATA CGGGACGGTA CGAGCAGCCT GCTGCGCATG 
TATCCTGGTC CCTTGCAGGG ACGCAAGTTG CACAAGGACA AGATCATCAG TTTCTGCAAG 
GAGCAGATCC GACTGGGGCC CACAAAGGGT TGCACCGCAC TGTATGCCAC GCAGAAGAAG 
CCACAGGGCA GCGTGACGAA ATACCGTGCC TCCCATGCGC TCATGTGGCA CCTGCTAATA 
CTTTTGCTGC GCCAAAATGG GTACATTGCG GACACGGATG TGGGCGATCT GCTGCTGGAG 
AACCAGCAAG AGTATCCCTA TGATCCCAGC GAGTTCGAAG CAGAGAACGA GCCAGATGCG 
GATGCCGAAC AGGATGCCGC TGCACCGGCG GACAAGACAG TCGATTCGGA TTTAGATAGC 
GAATCGGCAG CAGGAGTTAC ACCTGCGGAA CCACTTGCAG CAGGCGCGGC AACTAGCTCA 
ATCAATGGAG CTGATGCTGC AAACGCAGCA ACGCCTCTGT CGGAACAGGC GGCAACGGAC 
AAGTTCCGCA GCTATGTGCT GCGCGGAAAT GTCGAGGAGG CTCTCCAGTG GGCAACCGAC 
AACGGCCTGT GGACGCATGC ATTCTTCTTG GCCCTCTACG AGGATCGCTA TGCTCTCACT 
GACGTAGCCC AGAAGTTCCT CAATCGAGCG ATCAAGGCCA ACGATCCATT GCAGACACTA 
TACCAAATGA AGAGCTGCCA CACACCGGCG TGCGTCAGCC AGCTGAGGGA TGAACAGTGG 
GGTGACTGGC GATCGCATCT CTCCATCCTG GTGACGAACA AGAGTCGCCA GCCGGAGTAT 
GATCGCAGTT CGGTAGTGGC CCTTGGCGAT ACGCTTTTCC AGCGCGGCGA CATTTATGCG 
GCACACTTTT GCTATCTGGT GGCTCAGGAG GAGTTCGGAC GATACGATAG CTCTGCTACA 
GAGCTAACTA CTCTGACCGC CAATGTGCCC AGGCTTATTC TACTTGGCTC CTCGCACTAC 
AAGCACTTCA ACGAATTTGC CAGCAACGAG GCGATCATCA TGACCGAGAT CTATGAGTAT 
GCTCGATCGC TGTTCGATCC GAAGTTTAGC ATTGCCCACT TCCAACACTA CAAGTTCCTG 
CTAGCCACAA GGATCCTAGA TTATGGTCAG CATTTCCGCT GCACCAATTA TTTGGAACAA 
ATCGCCAGGC ACATCGAACT GAAGCCGGAA AGCTACGACA GTAATTTCAT TCAGCGGGTT 
TGCGGTTTGG CCGAACGCCT GCGCTACCAT GATCCCATTC TCATTAATCG TGTCTCATTT 
GCGAGTCCTC CGAATGCCAC TAGCAAGGAT AGTGCTGCTG CTGAAGAGAA GGCCTGGTTG 
CGTCAGCTTA GATCTCTGGC CTATGTGCAG CCCCAACAAG AGCAGCTGCA ACAACTGCAG 
CAGAATCAGC AAGTCCAGCA GGAGCAAAAC GACATCGATC AGCAGTTTGC AGAGGTGAAC 
AAGCAATTCA GGGAGCTAAA CATGCAATAC GAGAGCAACA GCAACATGGA GAATACGCTG 
CAGCCGCAGT TGCCACCGGT GGATCAACAG CCGCCGCAAC AGCAGCAACA AGTTTTATCG 
GAATCGTACC AACAGCCAAC ACAACAATAT TATGAGCCAC CACCTCAAGC GCCAACCGAA 
TCCGATCCCT ATGGACAGGG CCAACCGTCC TATTATGATC CCAATGCGGG GCAGCTTCAT 
TACGATCCAA ATGCTGCACA GCATCAATAC GACCAGCAGC AACCTCAACC ACAGCCGACT 
CCCAGCTATG GCCAAATAGA GCCAGGAACT GAGGCCTACC AACCTGGACA AGCAACAGCA 
GATCCAGCTG CGGTTCCCTC TGGCTACGGA TACGATTACT GGTCGGGCAC ACAGCAGCCA 
CCTTATGGTG ACGAGCCCAA TGAGAATCAA GCTGCACTAA AGGATGAAGA GGAGGAGGAT 
GAGGAGCAGG ATGAACAGCA GATATTGCAG TACGCTAAAC AGCAGATTTT GCAGCAAACC 
AAGCAGCAGC AGCTGCAGCA GGAGCAACAT ACTCGCAGCA ATGGCGCCGC CAGCATCAAC 
AATCGCTTCA AATCGAACGC TCTGAAGCAG GAACGCAGCA GCGGCTCATT GCGGAAAAGG 
CAAGAGGCAT CACGAACAGC AACAATAGCA GCGGCAGCAG CAGCTATTGC TCAGATAACA 
CCGGCAACAG TTGCAGCGGC AGCGACGCCA CCAGCAGCAT CGGCAAAGGC ATTCAATTTG 
AATGATGTAA GTAGCTTTTC CAGCACAAAT AAACGACATC AAGCTACCAG CAGCAGCAAC 
AATCGCAGCA GCAGCAACCA AGCAGCAGCA GCAGCAGCAG CAACAAAAGC TGCAGCAATA 
GCAACAGTAC CTGCTGCCCC TAATCAATTG CTAAGTTGTA GAAAGTCTAA CAGCCAGAAT 
AGTCCATACT TAGCCACGCC AAAAACGCTT ACCACGCCCA CACGTTTTAT TGCCGCTAAA 
GCAGCCACTG TTACAGCCAC GCCCACAGCG CCAGCCACTT CGACGACAAC GTCGACAACC 
ATAGGTGCTA GAGGATCATC TACCAAACGC GTTTACTTTA ATCTTCCGTC GACCATTGCT 
GAATCGGAGC AAGAGTTCGA ACATGAGCTG GATTATGATG AGGAGATGGA GGAGGAGAAC 
AGTATGGCTC TGGCGCAAGC TAAACCAAAC GTTCATCTAG CGCGAAAGCC ACATATTGTC 
TTTAATGGAA CGTATCCCAT TGATATGCCA CTGACCCAGG GGCAGAGTCA ACTCAAAAGC 
AACTCGTATG ATCCGGAGGC CAATGCAGAT CGTGGGGATC TACAGCGCTA TAGGAACTAC 
ATGCTCCACG ATTATGTTAT AGATGAGGAT GCCGAGGGCG AGGAGCAGGC CGAAGAAGAG 
GGCGAGGAGG ATGAGTATGG AGATGGTGAT GAGGATTTAT TCCCCTATGC CGACGACGAT 
GAGAGCTTCG ATGATGGCGA TTACTATACG CATCAACTGC TCAATAAGTA TGGGATTATA 
CCAAGCGGTG AGAAACAGAG AGTCGAGAGA ACACCGGCAT CCGTGGAGGC AGCTACCTCC 
GCGGCCCAGC CACAGCACGT GTGGTCCATG AAGGAGCTGA TAGCCAATCC AGCCATACCA 
CTTAACTACA AGAAAATGTG CTCCGAAGTG GAGCAGAGCC TCAGTCGATT CGAGAATTAT 
TTGGACAGCA AAAAGGAGCA ACAGGTGCCA CCGGGAGCCG CAGTGCGCAC CTTCGACATC 
GATGCACCCA CTCGAAGAGG CCACCGAAGC AGCACATCCA CAAATGCTGG CTTGGATCTT 
AGATCTAAGA AGAAATTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sec16 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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