Usp7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp7
Entrez Gene ID	32169
Full Name	Ubiquitin-specific protease 7
Synonyms	CG1490,Dmel\CG1490,Q9VYQ8,USP7,Usp-7
General protein information	Preferred Names Ubiquitin-specific protease 7 Names CG1490 gene product from transcript CG1490-RB CG1490-PB CG1490-PC Usp7-PB Usp7-PC ubiquitin-specific protease 7
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11A1-11A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_132551.3	NP_572779.2	Ubiquitin-specific protease 7, isoform B
NM_001298220.1	NP_001285149.1	Ubiquitin-specific protease 7, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP7	NP_003461.2
Pan troglodytes (chimpanzee)	USP7	XP_510806.2
	USP7	XP_002802434.1
Bos taurus (cattle)	USP7	XP_002698004.3
Mus musculus (house mouse)	Usp7	NP_001003918.2
Drosophila melanogaster (fruit fly)	Usp7	NP_572779.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008530	XP_316911.4
Eremothecium gossypii	AGOS_AGL357W	NP_986310.1
Oryza sativa (rice)	Os12g0489100	NP_001066793.1
Gallus gallus (chicken)	USP7	NP_989802.1
Saccharomyces cerevisiae (baker's yeast)	UBP15	NP_014033.1
Schizosaccharomyces pombe (fission yeast)	ubp15	NP_595341.1
Arabidopsis thaliana (thale cress)	UBP13	NP_001189864.1
Danio rerio (zebrafish)	usp7	XP_691215.4
Caenorhabditis elegans (roundworm)	math-33	NP_001024012.1
Neurospora crassa	NCU03797	XP_961186.2
Arabidopsis thaliana (thale cress)	UBP12	NP_850783.1
Canis lupus familiaris (dog)	USP7	XP_005621615.1
Rattus norvegicus (Norway rat)	Usp7	NP_001019961.1
Schizosaccharomyces pombe (fission yeast)	ubp5	NP_588211.1
Magnaporthe oryzae (rice blast fungus)	MGG_07468	XP_003711372.1
Xenopus tropicalis (tropical clawed frog)	usp7	XP_002939495.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

reveal a conserved mechanism by which Ci/Gli is stabilized by a deubiquitination enzyme and identify Usp7/HUASP as a critical regulator of Hh signaling and potential therapeutic target for Hh-related cancers
Title: Deubiquitination of Ci/Gli by Usp7/HAUSP Regulates Hedgehog Signaling.

Genetic and gene expression analyses suggested that GMPS/USP7 acts as a transcriptional corepressor
Title: Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes.

GMP synthetase stimulates histone H2B deubiquitylation by the epigenetic silencer USP7
Title: GMP synthetase stimulates histone H2B deubiquitylation by the epigenetic silencer USP7.

The following Usp7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01372	NM_132551.3 Latest version!	Drosophila melanogaster Ubiquitin-specific protease 7 (Usp7), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21438	NM_001298220.1 Latest version!	Drosophila melanogaster Ubiquitin-specific protease 7 (Usp7), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp7 ( NM_132551.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572779.2 Drosophila melanogaster Ubiquitin-specific protease 7 (Usp7), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01372
Clone ID Related Accession (Same CDS sequence)	NM_132551.3
Accession Version	NM_132551.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubiquitin-specific protease 7, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132551.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATTG AAACAGATCA GTCTATTGAA GCGATGGACA CCCAGGACAC CCAAGAGGTC 
GAGATCCTCA CCAGTGACCT ACAACAGACA CAGCAGCAGC GAAATTCACC GCCTCAATTG 
CCGAAATTCA AGAATCTTAT CCAGCCACAA TTGCATGCCG TTGGTGCAGT GACCCAGTTG 
CCCAGCGAGA ACGGCAATAT GCCGCCACAA CAACTATTGG CCGATAGCAG CTCCACTTCA 
TTCGGCGATG GCGAGGCCAT GGGCATTGAC GATGAGTCCA AGGAGGATCA GTTCCGTTCG 
GAGACGACAT TCTCCTTTAC CGTCGAGAAT GTGGTACAAT TGAAATCGCA GAGACTATCG 
CCGCCAGTTT ATGTCCGCAT GCTGCCATGG CGGATCATGG TCATACCCAA TGATCGAGCG 
CTCGGCTTCT TTCTGCAGTG CAACGGGGAG AATGATTCGC CCACCTGGTC GTGCAATGCC 
ATTGCCGAGC TGCGTTTGAA GTGCCACAAG CCGGATGCAC AGCCATTCAC ACGTGCCCGG 
ATTAAGCATT TGTTCTACTC CAAGGAGAAT GACTATGGCT ACTCGAATTT TATCACCTGG 
CAGGAGCTGA AGGATTCCGA AAAGAGCTAT GTGCACAATA ATAGCATTAC CCTGGAGGTT 
CACGTCGTTG CGGATGCACC GCATGGCGTT CTGTGGGACT CCAAGAAGCA CACAGGCTAT 
GTTGGCCTTA AAAATCAGGG CGCCACTTGC TATATGAACT CGCTGCTGCA GACATTGTAC 
TTCACCAATT CGCTAAGACT GTCTGTGTAT CGCATACCCA CGGAGGCTGA CGATAGTTCC 
AAATCCGTTG GATTATCGCT GCAGCGTGTG TTCCATGAAT TGCAGTTTGG TGACCGTCCC 
GTTGGCACCA AAAAGTTAAC CAAATCCTTT GGCTGGGAGA CGCTCGATTC GTTTATGCAG 
CACGATGTCC AGGAGTTTCT GCGCGTGCTG CTCGACAAGC TCGAGTCGAA GATGAAGGGC 
ACCATCCTGG AGGGCACCAT ACCGGGTCTG TTTGAGGGCA AAATGTCGTC GTACATTAAG 
TGCAAGAACG TCGATTACAA TAGCACACGC TACGAGACGT TCTACGACAT TCAGCTGAAC 
ATCAAGGATA AGAAGAACAT CTACGAATCC TTTCAGGATT ACGTAGCCCC CGAAACGCTG 
GAGGGTGACA ACAAATACGA TGCTGGTGTG CATGGCTTGC AGGAAGCCAG CAAGGGCGTC 
ATCTTCACTT CATTCCCACC CGTTTTGCAT CTGCACCTGA TGCGCTTCCA GTACGATCCC 
GTCACGGATA GCTCGATCAA GTACAACGAT CGTTTCGAGT TCTACGAACA CATCAATCTG 
GATCGCTATC TGGCCGAGAG TGAGAATACC TTGGCGGACT ACGTTCTACA CGCCGTGCTG 
GTGCATTCGG GCGATAACCA TGGCGGGCAC TATGTGGTCT TCATTAATCC AAAGGCTGAC 
GGACGTTGGT TTAAGTTCGA CGACGATGTG GTCTCCAGTT GCCGCAAACA GGAGGCGATC 
GAACAGAACT ACGGCGGCAT GGACGATGAG ATCTCGTTTC ATGCCAAGTG CAGCAATGCC 
TATATGCTGG TCTACATCCG ACAGTCGGAG TTGGATCGCG TCCTTGGCGA CATAACGGAA 
AGCGAGATAT CAAGCGATCT GGTGGAGCGG CTCGATCTGG AGAAACGAAT CGAAATGGCG 
CGACGCAAAG AGCGCGGCGA GGCCAACACA TATGTGTCCG TGCACGTAAT CCTCGAGGAG 
AACTTCGAGG AACAGCACAA GCGTCGTCTC TTCGATCTGG AGAAGGTCCA TCCGCGCGTC 
TTCCGCATCA AACAAAATCA GACTGTCGAC GAGCTGGTGG ACTTGTTTGT CAGGGGTTTC 
GGAGTGTCGC GCCAACGTAT GCGCATGTGG AACTTGTGCA CCGCCCAGAC GCAAAAGTTT 
TCGCATTTCG ATTTCGTGGC GGAGGGATCA CGCACCATCG AGCAGATATC AACGTCACAG 
AAACCGTGGG TCATCTGGCT GCAGCTGGCC TGGACGGATG TACCTGGTCC CCTGCCGCCC 
TTTAATCCCA AGACCGAATC CCTGCTGTTC CTCAAATACT ATGATCCGCG CAATAAGCGG 
CTAAATTATA TTGGATGTAC CCAACAGCCG CACACCCGCC GACTCATTGA TCTGGTGCCG 
GATGTTAATA GCAAACTGGG CTTCGAACCG GACACCGAGC TGACCATCTA CGATGAGTAT 
GCCGACAAGA AGCTGGTCAA TCTGAATGAG CCCATCGAGA GTGCGCTGTT CATACCGCAG 
GATCATCTGC AAGGCCACAT TTTAATCTTC GAGCGCGAAA ACGTTGACGC CAAACTCGAC 
CTGCCCACGG TAGGGGATTA CTTCCTGGAT CTAGTCTACC GCATCGAGAT CATCTTCAGC 
GACAAATGCA ATCCCAATGA ACCGGACTTT ACGCTGGAGC TGTCCAATCG CTATAATTAC 
GATCAGCTCG CCAATGCGGT GGCCGAGCGC TTGAATACCG ATCCACAGAA GCTGCAGTTC 
TTCATGTGCA TCAACAACTA CAAGGAGACG GCGGGCAATG CTGTACCCTA CACATTTAAG 
GGCACCATCA AGGATCTGGT TTCTTACACC AAACAGAGCT CAACCAAGCG TATCTTCTAT 
CAACGACTAT CGCTTAGCAT CCACGAGCTG GATAATAAGA AGCAGTTCAA GTGCGTCTGG 
GTGAGCAGTG ATCTTAAGGA CGAAAAGGAG CTGGTACTTT ACCCCAATAA GAACGATACG 
GTGAAAGGAT TGCTGGAGGA GGCGGCAAAG AAAATCTCGT TTGCGGAGAA CAGCCGTCGC 
AAATTGCGCC TGTTGAAGAT TAGCAATCAT AAGATTGTGG CGGTATGCAA GGACGATATA 
CCGCTGGATA CGCTGCTAAA GTCCAACGAG TCAATAACGA CCGCACAGGG CGCTCAAAAG 
ACTTTTCGCA TCGAGGAGGT GCCGGCGGAG GATATGCAAC TGGCGGAGAA TGAGTTCCTC 
ATACCAGTGG CCCATTTCAG CAAAGAGCTG TACAACTCAT TCGGCATACC CTTCCTAACC 
AAGGCGCGCC AAGGTGAGCC ATACGGTGCA CTTAAGCAGC GCATCCAGCG TCGTCTGAAT 
GTGCAGGACA AGGAGTGGGA AAACTACAAA TTCTGCGTAA TCTCTATGGG TCACAATGCG 
GATGTTAATG ACAATACGCC CGTTGATCTG GAGGTTTATC GCTCGTGGAC CAGCGGCCAG 
TTGCCCTTCT TTGGCTTGGA TCACATCAAC AAGTCGCGTA AGCGCAGTTC GCTTAACTTC 
TCCGAGAAGG CCATCAAGAT ATATAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572779.2
CDS	782..4171
Translation	MEIETDQSIEAMDTQDTQEVEILTSDLQQTQQQRNSPPQLPKFKNLIQPQLHAVGAVTQLPSENGNMPPQQLLADSSSTSFGDGEAMGIDDESKEDQFRSETTFSFTVENVVQLKSQRLSPPVYVRMLPWRIMVIPNDRALGFFLQCNGENDSPTWSCNAIAELRLKCHKPDAQPFTRARIKHLFYSKENDYGYSNFITWQELKDSEKSYVHNNSITLEVHVVADAPHGVLWDSKKHTGYVGLKNQGATCYMNSLLQTLYFTNSLRLSVYRIPTEADDSSKSVGLSLQRVFHELQFGDRPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTILEGTIPGLFEGKMSSYIKCKNVDYNSTRYETFYDIQLNIKDKKNIYESFQDYVAPETLEGDNKYDAGVHGLQEASKGVIFTSFPPVLHLHLMRFQYDPVTDSSIKYNDRFEFYEHINLDRYLAESENTLADYVLHAVLVHSGDNHGGHYVVFINPKADGRWFKFDDDVVSSCRKQEAIEQNYGGMDDEISFHAKCSNAYMLVYIRQSELDRVLGDITESEISSDLVERLDLEKRIEMARRKERGEANTYVSVHVILEENFEEQHKRRLFDLEKVHPRVFRIKQNQTVDELVDLFVRGFGVSRQRMRMWNLCTAQTQKFSHFDFVAEGSRTIEQISTSQKPWVIWLQLAWTDVPGPLPPFNPKTESLLFLKYYDPRNKRLNYIGCTQQPHTRRLIDLVPDVNSKLGFEPDTELTIYDEYADKKLVNLNEPIESALFIPQDHLQGHILIFERENVDAKLDLPTVGDYFLDLVYRIEIIFSDKCNPNEPDFTLELSNRYNYDQLANAVAERLNTDPQKLQFFMCINNYKETAGNAVPYTFKGTIKDLVSYTKQSSTKRIFYQRLSLSIHELDNKKQFKCVWVSSDLKDEKELVLYPNKNDTVKGLLEEAAKKISFAENSRRKLRLLKISNHKIVAVCKDDIPLDTLLKSNESITTAQGAQKTFRIEEVPAEDMQLAENEFLIPVAHFSKELYNSFGIPFLTKARQGEPYGALKQRIQRRLNVQDKEWENYKFCVISMGHNADVNDNTPVDLEVYRSWTSGQLPFFGLDHINKSRKRSSLNFSEKAIKIYN

Target ORF information:

RefSeq Version	NM_132551.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubiquitin-specific protease 7 (Usp7), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132551.3

ORF Insert Sequence:

ATGGAAATTG AAACAGATCA GTCTATTGAA GCGATGGACA CCCAGGACAC CCAAGAGGTC 
GAGATCCTCA CCAGTGACCT ACAACAGACA CAGCAGCAGC GAAATTCACC GCCTCAATTG 
CCGAAATTCA AGAATCTTAT CCAGCCACAA TTGCATGCCG TTGGTGCAGT GACCCAGTTG 
CCCAGCGAGA ACGGCAATAT GCCGCCACAA CAACTATTGG CCGATAGCAG CTCCACTTCA 
TTCGGCGATG GCGAGGCCAT GGGCATTGAC GATGAGTCCA AGGAGGATCA GTTCCGTTCG 
GAGACGACAT TCTCCTTTAC CGTCGAGAAT GTGGTACAAT TGAAATCGCA GAGACTATCG 
CCGCCAGTTT ATGTCCGCAT GCTGCCATGG CGGATCATGG TCATACCCAA TGATCGAGCG 
CTCGGCTTCT TTCTGCAGTG CAACGGGGAG AATGATTCGC CCACCTGGTC GTGCAATGCC 
ATTGCCGAGC TGCGTTTGAA GTGCCACAAG CCGGATGCAC AGCCATTCAC ACGTGCCCGG 
ATTAAGCATT TGTTCTACTC CAAGGAGAAT GACTATGGCT ACTCGAATTT TATCACCTGG 
CAGGAGCTGA AGGATTCCGA AAAGAGCTAT GTGCACAATA ATAGCATTAC CCTGGAGGTT 
CACGTCGTTG CGGATGCACC GCATGGCGTT CTGTGGGACT CCAAGAAGCA CACAGGCTAT 
GTTGGCCTTA AAAATCAGGG CGCCACTTGC TATATGAACT CGCTGCTGCA GACATTGTAC 
TTCACCAATT CGCTAAGACT GTCTGTGTAT CGCATACCCA CGGAGGCTGA CGATAGTTCC 
AAATCCGTTG GATTATCGCT GCAGCGTGTG TTCCATGAAT TGCAGTTTGG TGACCGTCCC 
GTTGGCACCA AAAAGTTAAC CAAATCCTTT GGCTGGGAGA CGCTCGATTC GTTTATGCAG 
CACGATGTCC AGGAGTTTCT GCGCGTGCTG CTCGACAAGC TCGAGTCGAA GATGAAGGGC 
ACCATCCTGG AGGGCACCAT ACCGGGTCTG TTTGAGGGCA AAATGTCGTC GTACATTAAG 
TGCAAGAACG TCGATTACAA TAGCACACGC TACGAGACGT TCTACGACAT TCAGCTGAAC 
ATCAAGGATA AGAAGAACAT CTACGAATCC TTTCAGGATT ACGTAGCCCC CGAAACGCTG 
GAGGGTGACA ACAAATACGA TGCTGGTGTG CATGGCTTGC AGGAAGCCAG CAAGGGCGTC 
ATCTTCACTT CATTCCCACC CGTTTTGCAT CTGCACCTGA TGCGCTTCCA GTACGATCCC 
GTCACGGATA GCTCGATCAA GTACAACGAT CGTTTCGAGT TCTACGAACA CATCAATCTG 
GATCGCTATC TGGCCGAGAG TGAGAATACC TTGGCGGACT ACGTTCTACA CGCCGTGCTG 
GTGCATTCGG GCGATAACCA TGGCGGGCAC TATGTGGTCT TCATTAATCC AAAGGCTGAC 
GGACGTTGGT TTAAGTTCGA CGACGATGTG GTCTCCAGTT GCCGCAAACA GGAGGCGATC 
GAACAGAACT ACGGCGGCAT GGACGATGAG ATCTCGTTTC ATGCCAAGTG CAGCAATGCC 
TATATGCTGG TCTACATCCG ACAGTCGGAG TTGGATCGCG TCCTTGGCGA CATAACGGAA 
AGCGAGATAT CAAGCGATCT GGTGGAGCGG CTCGATCTGG AGAAACGAAT CGAAATGGCG 
CGACGCAAAG AGCGCGGCGA GGCCAACACA TATGTGTCCG TGCACGTAAT CCTCGAGGAG 
AACTTCGAGG AACAGCACAA GCGTCGTCTC TTCGATCTGG AGAAGGTCCA TCCGCGCGTC 
TTCCGCATCA AACAAAATCA GACTGTCGAC GAGCTGGTGG ACTTGTTTGT CAGGGGTTTC 
GGAGTGTCGC GCCAACGTAT GCGCATGTGG AACTTGTGCA CCGCCCAGAC GCAAAAGTTT 
TCGCATTTCG ATTTCGTGGC GGAGGGATCA CGCACCATCG AGCAGATATC AACGTCACAG 
AAACCGTGGG TCATCTGGCT GCAGCTGGCC TGGACGGATG TACCTGGTCC CCTGCCGCCC 
TTTAATCCCA AGACCGAATC CCTGCTGTTC CTCAAATACT ATGATCCGCG CAATAAGCGG 
CTAAATTATA TTGGATGTAC CCAACAGCCG CACACCCGCC GACTCATTGA TCTGGTGCCG 
GATGTTAATA GCAAACTGGG CTTCGAACCG GACACCGAGC TGACCATCTA CGATGAGTAT 
GCCGACAAGA AGCTGGTCAA TCTGAATGAG CCCATCGAGA GTGCGCTGTT CATACCGCAG 
GATCATCTGC AAGGCCACAT TTTAATCTTC GAGCGCGAAA ACGTTGACGC CAAACTCGAC 
CTGCCCACGG TAGGGGATTA CTTCCTGGAT CTAGTCTACC GCATCGAGAT CATCTTCAGC 
GACAAATGCA ATCCCAATGA ACCGGACTTT ACGCTGGAGC TGTCCAATCG CTATAATTAC 
GATCAGCTCG CCAATGCGGT GGCCGAGCGC TTGAATACCG ATCCACAGAA GCTGCAGTTC 
TTCATGTGCA TCAACAACTA CAAGGAGACG GCGGGCAATG CTGTACCCTA CACATTTAAG 
GGCACCATCA AGGATCTGGT TTCTTACACC AAACAGAGCT CAACCAAGCG TATCTTCTAT 
CAACGACTAT CGCTTAGCAT CCACGAGCTG GATAATAAGA AGCAGTTCAA GTGCGTCTGG 
GTGAGCAGTG ATCTTAAGGA CGAAAAGGAG CTGGTACTTT ACCCCAATAA GAACGATACG 
GTGAAAGGAT TGCTGGAGGA GGCGGCAAAG AAAATCTCGT TTGCGGAGAA CAGCCGTCGC 
AAATTGCGCC TGTTGAAGAT TAGCAATCAT AAGATTGTGG CGGTATGCAA GGACGATATA 
CCGCTGGATA CGCTGCTAAA GTCCAACGAG TCAATAACGA CCGCACAGGG CGCTCAAAAG 
ACTTTTCGCA TCGAGGAGGT GCCGGCGGAG GATATGCAAC TGGCGGAGAA TGAGTTCCTC 
ATACCAGTGG CCCATTTCAG CAAAGAGCTG TACAACTCAT TCGGCATACC CTTCCTAACC 
AAGGCGCGCC AAGGTGAGCC ATACGGTGCA CTTAAGCAGC GCATCCAGCG TCGTCTGAAT 
GTGCAGGACA AGGAGTGGGA AAACTACAAA TTCTGCGTAA TCTCTATGGG TCACAATGCG 
GATGTTAATG ACAATACGCC CGTTGATCTG GAGGTTTATC GCTCGTGGAC CAGCGGCCAG 
TTGCCCTTCT TTGGCTTGGA TCACATCAAC AAGTCGCGTA AGCGCAGTTC GCTTAACTTC 
TCCGAGAAGG CCATCAAGAT ATATAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp7 ( NM_001298220.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285149.1 Drosophila melanogaster Ubiquitin-specific protease 7 (Usp7), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa21438
Clone ID Related Accession (Same CDS sequence)	NM_001298220.1
Accession Version	NM_001298220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Ubiquitin-specific protease 7, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAATTG AAACAGATCA GTCTATTGAA GCGATGGACA CCCAGGACAC CCAAGAGGTC 
GAGATCCTCA CCAGTGACCT ACAACAGACA CAGCAGCAGC GAAATTCACC GCCTCAATTG 
CCGAAATTCA AGAATCTTAT CCAGCCACAA TTGCATGCCG TTGGTGCAGT GACCCAGTTG 
CCCAGCGAGA ACGGCAATAT GCCGCCACAA CAACTATTGG CCGATAGCAG CTCCACTTCA 
TTCGGCGATG GCGAGGCCAT GGGCATTGAC GATGAGTCCA AGGAGGATCA GTTCCGTTCG 
GAGACGACAT TCTCCTTTAC CGTCGAGAAT GTGGTACAAT TGAAATCGCA GAGACTATCG 
CCGCCAGTTT ATGTCCGCAT GCTGCCATGG CGGATCATGG TCATACCCAA TGATCGAGCG 
CTCGGCTTCT TTCTGCAGTG CAACGGGGAG AATGATTCGC CCACCTGGTC GTGCAATGCC 
ATTGCCGAGC TGCGTTTGAA GTGCCACAAG CCGGATGCAC AGCCATTCAC ACGTGCCCGG 
ATTAAGCATT TGTTCTACTC CAAGGAGAAT GACTATGGCT ACTCGAATTT TATCACCTGG 
CAGGAGCTGA AGGATTCCGA AAAGAGCTAT GTGCACAATA ATAGCATTAC CCTGGAGGTT 
CACGTCGTTG CGGATGCACC GCATGGCGTT CTGTGGGACT CCAAGAAGCA CACAGGCTAT 
GTTGGCCTTA AAAATCAGGG CGCCACTTGC TATATGAACT CGCTGCTGCA GACATTGTAC 
TTCACCAATT CGCTAAGACT GTCTGTGTAT CGCATACCCA CGGAGGCTGA CGATAGTTCC 
AAATCCGTTG GATTATCGCT GCAGCGTGTG TTCCATGAAT TGCAGTTTGG TGACCGTCCC 
GTTGGCACCA AAAAGTTAAC CAAATCCTTT GGCTGGGAGA CGCTCGATTC GTTTATGCAG 
CACGATGTCC AGGAGTTTCT GCGCGTGCTG CTCGACAAGC TCGAGTCGAA GATGAAGGGC 
ACCATCCTGG AGGGCACCAT ACCGGGTCTG TTTGAGGGCA AAATGTCGTC GTACATTAAG 
TGCAAGAACG TCGATTACAA TAGCACACGC TACGAGACGT TCTACGACAT TCAGCTGAAC 
ATCAAGGATA AGAAGAACAT CTACGAATCC TTTCAGGATT ACGTAGCCCC CGAAACGCTG 
GAGGGTGACA ACAAATACGA TGCTGGTGTG CATGGCTTGC AGGAAGCCAG CAAGGGCGTC 
ATCTTCACTT CATTCCCACC CGTTTTGCAT CTGCACCTGA TGCGCTTCCA GTACGATCCC 
GTCACGGATA GCTCGATCAA GTACAACGAT CGTTTCGAGT TCTACGAACA CATCAATCTG 
GATCGCTATC TGGCCGAGAG TGAGAATACC TTGGCGGACT ACGTTCTACA CGCCGTGCTG 
GTGCATTCGG GCGATAACCA TGGCGGGCAC TATGTGGTCT TCATTAATCC AAAGGCTGAC 
GGACGTTGGT TTAAGTTCGA CGACGATGTG GTCTCCAGTT GCCGCAAACA GGAGGCGATC 
GAACAGAACT ACGGCGGCAT GGACGATGAG ATCTCGTTTC ATGCCAAGTG CAGCAATGCC 
TATATGCTGG TCTACATCCG ACAGTCGGAG TTGGATCGCG TCCTTGGCGA CATAACGGAA 
AGCGAGATAT CAAGCGATCT GGTGGAGCGG CTCGATCTGG AGAAACGAAT CGAAATGGCG 
CGACGCAAAG AGCGCGGCGA GGCCAACACA TATGTGTCCG TGCACGTAAT CCTCGAGGAG 
AACTTCGAGG AACAGCACAA GCGTCGTCTC TTCGATCTGG AGAAGGTCCA TCCGCGCGTC 
TTCCGCATCA AACAAAATCA GACTGTCGAC GAGCTGGTGG ACTTGTTTGT CAGGGGTTTC 
GGAGTGTCGC GCCAACGTAT GCGCATGTGG AACTTGTGCA CCGCCCAGAC GCAAAAGTTT 
TCGCATTTCG ATTTCGTGGC GGAGGGATCA CGCACCATCG AGCAGATATC AACGTCACAG 
AAACCGTGGG TCATCTGGCT GCAGCTGGCC TGGACGGATG TACCTGGTCC CCTGCCGCCC 
TTTAATCCCA AGACCGAATC CCTGCTGTTC CTCAAATACT ATGATCCGCG CAATAAGCGG 
CTAAATTATA TTGGATGTAC CCAACAGCCG CACACCCGCC GACTCATTGA TCTGGTGCCG 
GATGTTAATA GCAAACTGGG CTTCGAACCG GACACCGAGC TGACCATCTA CGATGAGTAT 
GCCGACAAGA AGCTGGTCAA TCTGAATGAG CCCATCGAGA GTGCGCTGTT CATACCGCAG 
GATCATCTGC AAGGCCACAT TTTAATCTTC GAGCGCGAAA ACGTTGACGC CAAACTCGAC 
CTGCCCACGG TAGGGGATTA CTTCCTGGAT CTAGTCTACC GCATCGAGAT CATCTTCAGC 
GACAAATGCA ATCCCAATGA ACCGGACTTT ACGCTGGAGC TGTCCAATCG CTATAATTAC 
GATCAGCTCG CCAATGCGGT GGCCGAGCGC TTGAATACCG ATCCACAGAA GCTGCAGTTC 
TTCATGTGCA TCAACAACTA CAAGGAGACG GCGGGCAATG CTGTACCCTA CACATTTAAG 
GATCTGGTTT CTTACACCAA ACAGAGCTCA ACCAAGCGTA TCTTCTATCA ACGACTATCG 
CTTAGCATCC ACGAGCTGGA TAATAAGAAG CAGTTCAAGT GCGTCTGGGT GAGCAGTGAT 
CTTAAGGACG AAAAGGAGCT GGTACTTTAC CCCAATAAGA ACGATACGGT GAAAGGATTG 
CTGGAGGAGG CGGCAAAGAA AATCTCGTTT GCGGAGAACA GCCGTCGCAA ATTGCGCCTG 
TTGAAGATTA GCAATCATAA GATTGTGGCG GTATGCAAGG ACGATATACC GCTGGATACG 
CTGCTAAAGT CCAACGAGTC AATAACGACC GCACAGGGCG CTCAAAAGAC TTTTCGCATC 
GAGGAGGTGC CGGCGGAGGA TATGCAACTG GCGGAGAATG AGTTCCTCAT ACCAGTGGCC 
CATTTCAGCA AAGAGCTGTA CAACTCATTC GGCATACCCT TCCTAACCAA GGCGCGCCAA 
GGTGAGCCAT ACGGTGCACT TAAGCAGCGC ATCCAGCGTC GTCTGAATGT GCAGGACAAG 
GAGTGGGAAA ACTACAAATT CTGCGTAATC TCTATGGGTC ACAATGCGGA TGTTAATGAC 
AATACGCCCG TTGATCTGGA GGTTTATCGC TCGTGGACCA GCGGCCAGTT GCCCTTCTTT 
GGCTTGGATC ACATCAACAA GTCGCGTAAG CGCAGTTCGC TTAACTTCTC CGAGAAGGCC 
ATCAAGATAT ATAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285149.1
CDS	782..4159
Translation	MEIETDQSIEAMDTQDTQEVEILTSDLQQTQQQRNSPPQLPKFKNLIQPQLHAVGAVTQLPSENGNMPPQQLLADSSSTSFGDGEAMGIDDESKEDQFRSETTFSFTVENVVQLKSQRLSPPVYVRMLPWRIMVIPNDRALGFFLQCNGENDSPTWSCNAIAELRLKCHKPDAQPFTRARIKHLFYSKENDYGYSNFITWQELKDSEKSYVHNNSITLEVHVVADAPHGVLWDSKKHTGYVGLKNQGATCYMNSLLQTLYFTNSLRLSVYRIPTEADDSSKSVGLSLQRVFHELQFGDRPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTILEGTIPGLFEGKMSSYIKCKNVDYNSTRYETFYDIQLNIKDKKNIYESFQDYVAPETLEGDNKYDAGVHGLQEASKGVIFTSFPPVLHLHLMRFQYDPVTDSSIKYNDRFEFYEHINLDRYLAESENTLADYVLHAVLVHSGDNHGGHYVVFINPKADGRWFKFDDDVVSSCRKQEAIEQNYGGMDDEISFHAKCSNAYMLVYIRQSELDRVLGDITESEISSDLVERLDLEKRIEMARRKERGEANTYVSVHVILEENFEEQHKRRLFDLEKVHPRVFRIKQNQTVDELVDLFVRGFGVSRQRMRMWNLCTAQTQKFSHFDFVAEGSRTIEQISTSQKPWVIWLQLAWTDVPGPLPPFNPKTESLLFLKYYDPRNKRLNYIGCTQQPHTRRLIDLVPDVNSKLGFEPDTELTIYDEYADKKLVNLNEPIESALFIPQDHLQGHILIFERENVDAKLDLPTVGDYFLDLVYRIEIIFSDKCNPNEPDFTLELSNRYNYDQLANAVAERLNTDPQKLQFFMCINNYKETAGNAVPYTFKDLVSYTKQSSTKRIFYQRLSLSIHELDNKKQFKCVWVSSDLKDEKELVLYPNKNDTVKGLLEEAAKKISFAENSRRKLRLLKISNHKIVAVCKDDIPLDTLLKSNESITTAQGAQKTFRIEEVPAEDMQLAENEFLIPVAHFSKELYNSFGIPFLTKARQGEPYGALKQRIQRRLNVQDKEWENYKFCVISMGHNADVNDNTPVDLEVYRSWTSGQLPFFGLDHINKSRKRSSLNFSEKAIKIYN

Target ORF information:

RefSeq Version	NM_001298220.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Ubiquitin-specific protease 7 (Usp7), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298220.1

ORF Insert Sequence:

ATGGAAATTG AAACAGATCA GTCTATTGAA GCGATGGACA CCCAGGACAC CCAAGAGGTC 
GAGATCCTCA CCAGTGACCT ACAACAGACA CAGCAGCAGC GAAATTCACC GCCTCAATTG 
CCGAAATTCA AGAATCTTAT CCAGCCACAA TTGCATGCCG TTGGTGCAGT GACCCAGTTG 
CCCAGCGAGA ACGGCAATAT GCCGCCACAA CAACTATTGG CCGATAGCAG CTCCACTTCA 
TTCGGCGATG GCGAGGCCAT GGGCATTGAC GATGAGTCCA AGGAGGATCA GTTCCGTTCG 
GAGACGACAT TCTCCTTTAC CGTCGAGAAT GTGGTACAAT TGAAATCGCA GAGACTATCG 
CCGCCAGTTT ATGTCCGCAT GCTGCCATGG CGGATCATGG TCATACCCAA TGATCGAGCG 
CTCGGCTTCT TTCTGCAGTG CAACGGGGAG AATGATTCGC CCACCTGGTC GTGCAATGCC 
ATTGCCGAGC TGCGTTTGAA GTGCCACAAG CCGGATGCAC AGCCATTCAC ACGTGCCCGG 
ATTAAGCATT TGTTCTACTC CAAGGAGAAT GACTATGGCT ACTCGAATTT TATCACCTGG 
CAGGAGCTGA AGGATTCCGA AAAGAGCTAT GTGCACAATA ATAGCATTAC CCTGGAGGTT 
CACGTCGTTG CGGATGCACC GCATGGCGTT CTGTGGGACT CCAAGAAGCA CACAGGCTAT 
GTTGGCCTTA AAAATCAGGG CGCCACTTGC TATATGAACT CGCTGCTGCA GACATTGTAC 
TTCACCAATT CGCTAAGACT GTCTGTGTAT CGCATACCCA CGGAGGCTGA CGATAGTTCC 
AAATCCGTTG GATTATCGCT GCAGCGTGTG TTCCATGAAT TGCAGTTTGG TGACCGTCCC 
GTTGGCACCA AAAAGTTAAC CAAATCCTTT GGCTGGGAGA CGCTCGATTC GTTTATGCAG 
CACGATGTCC AGGAGTTTCT GCGCGTGCTG CTCGACAAGC TCGAGTCGAA GATGAAGGGC 
ACCATCCTGG AGGGCACCAT ACCGGGTCTG TTTGAGGGCA AAATGTCGTC GTACATTAAG 
TGCAAGAACG TCGATTACAA TAGCACACGC TACGAGACGT TCTACGACAT TCAGCTGAAC 
ATCAAGGATA AGAAGAACAT CTACGAATCC TTTCAGGATT ACGTAGCCCC CGAAACGCTG 
GAGGGTGACA ACAAATACGA TGCTGGTGTG CATGGCTTGC AGGAAGCCAG CAAGGGCGTC 
ATCTTCACTT CATTCCCACC CGTTTTGCAT CTGCACCTGA TGCGCTTCCA GTACGATCCC 
GTCACGGATA GCTCGATCAA GTACAACGAT CGTTTCGAGT TCTACGAACA CATCAATCTG 
GATCGCTATC TGGCCGAGAG TGAGAATACC TTGGCGGACT ACGTTCTACA CGCCGTGCTG 
GTGCATTCGG GCGATAACCA TGGCGGGCAC TATGTGGTCT TCATTAATCC AAAGGCTGAC 
GGACGTTGGT TTAAGTTCGA CGACGATGTG GTCTCCAGTT GCCGCAAACA GGAGGCGATC 
GAACAGAACT ACGGCGGCAT GGACGATGAG ATCTCGTTTC ATGCCAAGTG CAGCAATGCC 
TATATGCTGG TCTACATCCG ACAGTCGGAG TTGGATCGCG TCCTTGGCGA CATAACGGAA 
AGCGAGATAT CAAGCGATCT GGTGGAGCGG CTCGATCTGG AGAAACGAAT CGAAATGGCG 
CGACGCAAAG AGCGCGGCGA GGCCAACACA TATGTGTCCG TGCACGTAAT CCTCGAGGAG 
AACTTCGAGG AACAGCACAA GCGTCGTCTC TTCGATCTGG AGAAGGTCCA TCCGCGCGTC 
TTCCGCATCA AACAAAATCA GACTGTCGAC GAGCTGGTGG ACTTGTTTGT CAGGGGTTTC 
GGAGTGTCGC GCCAACGTAT GCGCATGTGG AACTTGTGCA CCGCCCAGAC GCAAAAGTTT 
TCGCATTTCG ATTTCGTGGC GGAGGGATCA CGCACCATCG AGCAGATATC AACGTCACAG 
AAACCGTGGG TCATCTGGCT GCAGCTGGCC TGGACGGATG TACCTGGTCC CCTGCCGCCC 
TTTAATCCCA AGACCGAATC CCTGCTGTTC CTCAAATACT ATGATCCGCG CAATAAGCGG 
CTAAATTATA TTGGATGTAC CCAACAGCCG CACACCCGCC GACTCATTGA TCTGGTGCCG 
GATGTTAATA GCAAACTGGG CTTCGAACCG GACACCGAGC TGACCATCTA CGATGAGTAT 
GCCGACAAGA AGCTGGTCAA TCTGAATGAG CCCATCGAGA GTGCGCTGTT CATACCGCAG 
GATCATCTGC AAGGCCACAT TTTAATCTTC GAGCGCGAAA ACGTTGACGC CAAACTCGAC 
CTGCCCACGG TAGGGGATTA CTTCCTGGAT CTAGTCTACC GCATCGAGAT CATCTTCAGC 
GACAAATGCA ATCCCAATGA ACCGGACTTT ACGCTGGAGC TGTCCAATCG CTATAATTAC 
GATCAGCTCG CCAATGCGGT GGCCGAGCGC TTGAATACCG ATCCACAGAA GCTGCAGTTC 
TTCATGTGCA TCAACAACTA CAAGGAGACG GCGGGCAATG CTGTACCCTA CACATTTAAG 
GATCTGGTTT CTTACACCAA ACAGAGCTCA ACCAAGCGTA TCTTCTATCA ACGACTATCG 
CTTAGCATCC ACGAGCTGGA TAATAAGAAG CAGTTCAAGT GCGTCTGGGT GAGCAGTGAT 
CTTAAGGACG AAAAGGAGCT GGTACTTTAC CCCAATAAGA ACGATACGGT GAAAGGATTG 
CTGGAGGAGG CGGCAAAGAA AATCTCGTTT GCGGAGAACA GCCGTCGCAA ATTGCGCCTG 
TTGAAGATTA GCAATCATAA GATTGTGGCG GTATGCAAGG ACGATATACC GCTGGATACG 
CTGCTAAAGT CCAACGAGTC AATAACGACC GCACAGGGCG CTCAAAAGAC TTTTCGCATC 
GAGGAGGTGC CGGCGGAGGA TATGCAACTG GCGGAGAATG AGTTCCTCAT ACCAGTGGCC 
CATTTCAGCA AAGAGCTGTA CAACTCATTC GGCATACCCT TCCTAACCAA GGCGCGCCAA 
GGTGAGCCAT ACGGTGCACT TAAGCAGCGC ATCCAGCGTC GTCTGAATGT GCAGGACAAG 
GAGTGGGAAA ACTACAAATT CTGCGTAATC TCTATGGGTC ACAATGCGGA TGTTAATGAC 
AATACGCCCG TTGATCTGGA GGTTTATCGC TCGTGGACCA GCGGCCAGTT GCCCTTCTTT 
GGCTTGGATC ACATCAACAA GTCGCGTAAG CGCAGTTCGC TTAACTTCTC CGAGAAGGCC 
ATCAAGATAT ATAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Usp7 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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