Sk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sk1
Entrez Gene ID	32089
Full Name	Sphingosine kinase 1
Synonyms	CG1747,Dmel\CG1747,SK1,Sphk1
General protein information	Preferred Names Sphingosine kinase 1 Names CG1747 gene product from transcript CG1747-RB CG1747-PA CG1747-PB Sk1-PA Sk1-PB sphingosine kinase 1 sphingosine kinase I
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10B13-10B13

mRNA and Protein(s)

mRNA	Protein	Name
NM_167286.2	NP_727528.1	sphingosine kinase 1, isoform B
NM_132489.2	NP_572717.1	sphingosine kinase 1, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SPHK2	XP_002695244.2
Homo sapiens (human)	SPHK2	NP_064511.2
Pan troglodytes (chimpanzee)	SPHK2	XP_001171404.2
Danio rerio (zebrafish)	sphk2	XP_003200262.2
	SPHK2	XP_001113908.1
Mus musculus (house mouse)	Sphk2	NP_064395.2
Rattus norvegicus (Norway rat)	Sphk2	NP_001012066.1
Drosophila melanogaster (fruit fly)	Sk1	NP_572717.1
Caenorhabditis elegans (roundworm)	sphk-1	NP_001022018.1
Xenopus tropicalis (tropical clawed frog)	sphk2	XP_002938325.1
Canis lupus familiaris (dog)	SPHK2	XP_005616402.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sk1 and Sk2 are not redundant in biological function and are essential for diverse physiological functions in Drosophila
Title: Characterization of the Drosophila sphingosine kinases and requirement for Sk2 in normal reproductive function.

The following Sk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03515	NM_132489.2 Latest version!	Drosophila melanogaster sphingosine kinase 1, transcript variant A (Sk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03515	NM_167286.2 Latest version!	Drosophila melanogaster sphingosine kinase 1, transcript variant B (Sk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sk1 ( NM_167286.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727528.1 Drosophila melanogaster sphingosine kinase 1, transcript variant B (Sk1), mRNA.

Price & Availability↑

CloneID	OFa03515
Clone ID Related Accession (Same CDS sequence)	NM_167286.2 , NM_132489.2
Accession Version	NM_167286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sphingosine kinase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167286.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGCCA ACACAGGGAC GTCGGGTGAA AATCTGATGG GAAATGGTGG AAAGACGCAT 
GAACCCTCCA CGCCCACTTC GGATACGGAA GTGGCCAGCG GCACGCCAAC GGAACTGAGC 
GAAATCTTCT TCGTGGACAA TAGCCGGCGC AAGCAGAGCA TCAAAATCCA GGTGAAACTA 
TGCCCGGAAG GCGTTTACCT GCGACGCGAA ACCGAGGAAG ATGATCACAT CAATGAGCAG 
CTGATCAGGA TCGATGATAT CATAGGATCA CGCTACGGAC GGCGTTTGAA GAAACGAGCC 
CGAGGCGGTC TAAACTCCTG CCGCAATCCA AATGTTCCGG GCCAGGAGGC GGATTCGGAA 
CCGGATAGCG ATAATAGCGC CTATTTGTAC ATCTATGCAT ATTTGAAGAA GGAGAAACCG 
TTGCGACGTG TCCAAACGCT CCGGATTCTA CGCTTTCGTT CGAGCAATGA CTACGGAGTG 
AATCTACAGA CCGCCGAGAT GTGGCATCAT ACGATTCGAA AGCACAAGCG TGGCAATGGC 
AGCAGTTCGC CCGCCGATTG TGGCAAACAG TTGCTCATCC TACTGAATCC GAAATCCGGT 
TCGGGCAAAG GGCGTGAGCT CTTCCAGAAA CAGGTGGCAC CTTTGCTGAC GGAAGCAGAG 
GTGCAATACG ATCTCCAGAT CACCACACAT CCGCAGTATG CCAAGGAGTT CGTGCGGACC 
AGAAGGGATC TGCTGACACG CTATTCGGGC ATTGTGGTTG CCTCCGGCGA TGGTCTATTC 
TACGAAGTGC TCAATGGGCT AATGGAACGC ATGGATTGGC GCCGAGCCTG CAGGGAGCTA 
CCGCTTGGCA TTATACCATG TGGTTCCGGG AATGGTCTGG CCAAAAGTGT GGCCCATCAT 
TGCAATGAAC CGTACGAACC GAAGCCCATT CTCCACGCCA CCTTGACCTG CATGGCGGGC 
AAAAGTACAC CCATGGATGT GGTCAGAGTG GAGCTGGCGA CGCGGGACAA GCACTTTGTG 
ATGTACTCCT TCCTGTCGGT GGGCTGGGGT CTGATAGCCG ACATCGATAT AGAGAGCGAG 
CGATTGAGAT CGATTGGAGC GCAAAGGTTT ACGCTGTGGG CCATCAAGCG ATTGATCGGG 
CTGCGCAGCT ACAAAGGCCG AGTGTCCTAT CTACTGGGCA AGGGCAAGAA GGAACCACCA 
GTGGAAGCGG CTCGAGAGTT GCCTGCAGAA TCAACGGCTG CAGGAATCCG CTCATCTCTG 
CCTCTGAATG CCGGGGAATT CCATGATCTA CCCGAGGAGG AGGAGGGGGA GGCGGTCTTG 
GATGGAGAAC AGTTCGCCGA TGCCATATCT TTGGATCGTT CGGTTTACCG CCAGCATGCC 
GACAGTTGGC ACTCGGCCAT GTCCAGGCGA ACGGCATATT ACTCCCTGGG CGGACCCAGT 
ATGCGATCCA ATCGCAGCCG GATGAGCATT AGCCAGCGGA TCGAGGCAGC AAATGCGGAA 
TTCGCTGAGA GGGTGCCAAC GGGCACCATT CCACCATTAC AGATGCCACT GCTCAGCAGC 
GATGGTTGGA TCTGCGAGGA TGGTGACTTT GTGATGGTCC ATGCCGCCTA TACCACCCAT 
CTCTCCTCCG ATGTCTTCTT TGCGCCCGAA TCCCGTCTGG ACGATGGCCT CATCTACCTG 
GTGATCATCC GGAGAGGCGT TAGTCGCCAT CAGCTGCTCA ATTTCATGCT GAACCTAAAC 
GCAGGCACCC ATCTGCCCAT CGGCGAGGAT CCGTTCATCA AGGTGGTGCC TTGTCGGGCA 
TTCCGCATCG AGCCGAGCAG CTCCGATGGC ATCCTGGTGG TGGACGGCGA GCGGGTGGAA 
TATGGACCCA TTCAGGCGGA GGTTATGCCC GGCCTGATCA ATGTGATGAC CACCAGTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727528.1
CDS	237..2162
Translation	MTANTGTSGENLMGNGGKTHEPSTPTSDTEVASGTPTELSEIFFVDNSRRKQSIKIQVKLCPEGVYLRRETEEDDHINEQLIRIDDIIGSRYGRRLKKRARGGLNSCRNPNVPGQEADSEPDSDNSAYLYIYAYLKKEKPLRRVQTLRILRFRSSNDYGVNLQTAEMWHHTIRKHKRGNGSSSPADCGKQLLILLNPKSGSGKGRELFQKQVAPLLTEAEVQYDLQITTHPQYAKEFVRTRRDLLTRYSGIVVASGDGLFYEVLNGLMERMDWRRACRELPLGIIPCGSGNGLAKSVAHHCNEPYEPKPILHATLTCMAGKSTPMDVVRVELATRDKHFVMYSFLSVGWGLIADIDIESERLRSIGAQRFTLWAIKRLIGLRSYKGRVSYLLGKGKKEPPVEAARELPAESTAAGIRSSLPLNAGEFHDLPEEEEGEAVLDGEQFADAISLDRSVYRQHADSWHSAMSRRTAYYSLGGPSMRSNRSRMSISQRIEAANAEFAERVPTGTIPPLQMPLLSSDGWICEDGDFVMVHAAYTTHLSSDVFFAPESRLDDGLIYLVIIRRGVSRHQLLNFMLNLNAGTHLPIGEDPFIKVVPCRAFRIEPSSSDGILVVDGERVEYGPIQAEVMPGLINVMTTSGQ

Target ORF information:

RefSeq Version	NM_167286.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sphingosine kinase 1, transcript variant B (Sk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167286.2

ORF Insert Sequence:

ATGACGGCCA ACACAGGGAC GTCGGGTGAA AATCTGATGG GAAATGGTGG AAAGACGCAT 
GAACCCTCCA CGCCCACTTC GGATACGGAA GTGGCCAGCG GCACGCCAAC GGAACTGAGC 
GAAATCTTCT TCGTGGACAA TAGCCGGCGC AAGCAGAGCA TCAAAATCCA GGTGAAACTA 
TGCCCGGAAG GCGTTTACCT GCGACGCGAA ACCGAGGAAG ATGATCACAT CAATGAGCAG 
CTGATCAGGA TCGATGATAT CATAGGATCA CGCTACGGAC GGCGTTTGAA GAAACGAGCC 
CGAGGCGGTC TAAACTCCTG CCGCAATCCA AATGTTCCGG GCCAGGAGGC GGATTCGGAA 
CCGGATAGCG ATAATAGCGC CTATTTGTAC ATCTATGCAT ATTTGAAGAA GGAGAAACCG 
TTGCGACGTG TCCAAACGCT CCGGATTCTA CGCTTTCGTT CGAGCAATGA CTACGGAGTG 
AATCTACAGA CCGCCGAGAT GTGGCATCAT ACGATTCGAA AGCACAAGCG TGGCAATGGC 
AGCAGTTCGC CCGCCGATTG TGGCAAACAG TTGCTCATCC TACTGAATCC GAAATCCGGT 
TCGGGCAAAG GGCGTGAGCT CTTCCAGAAA CAGGTGGCAC CTTTGCTGAC GGAAGCAGAG 
GTGCAATACG ATCTCCAGAT CACCACACAT CCGCAGTATG CCAAGGAGTT CGTGCGGACC 
AGAAGGGATC TGCTGACACG CTATTCGGGC ATTGTGGTTG CCTCCGGCGA TGGTCTATTC 
TACGAAGTGC TCAATGGGCT AATGGAACGC ATGGATTGGC GCCGAGCCTG CAGGGAGCTA 
CCGCTTGGCA TTATACCATG TGGTTCCGGG AATGGTCTGG CCAAAAGTGT GGCCCATCAT 
TGCAATGAAC CGTACGAACC GAAGCCCATT CTCCACGCCA CCTTGACCTG CATGGCGGGC 
AAAAGTACAC CCATGGATGT GGTCAGAGTG GAGCTGGCGA CGCGGGACAA GCACTTTGTG 
ATGTACTCCT TCCTGTCGGT GGGCTGGGGT CTGATAGCCG ACATCGATAT AGAGAGCGAG 
CGATTGAGAT CGATTGGAGC GCAAAGGTTT ACGCTGTGGG CCATCAAGCG ATTGATCGGG 
CTGCGCAGCT ACAAAGGCCG AGTGTCCTAT CTACTGGGCA AGGGCAAGAA GGAACCACCA 
GTGGAAGCGG CTCGAGAGTT GCCTGCAGAA TCAACGGCTG CAGGAATCCG CTCATCTCTG 
CCTCTGAATG CCGGGGAATT CCATGATCTA CCCGAGGAGG AGGAGGGGGA GGCGGTCTTG 
GATGGAGAAC AGTTCGCCGA TGCCATATCT TTGGATCGTT CGGTTTACCG CCAGCATGCC 
GACAGTTGGC ACTCGGCCAT GTCCAGGCGA ACGGCATATT ACTCCCTGGG CGGACCCAGT 
ATGCGATCCA ATCGCAGCCG GATGAGCATT AGCCAGCGGA TCGAGGCAGC AAATGCGGAA 
TTCGCTGAGA GGGTGCCAAC GGGCACCATT CCACCATTAC AGATGCCACT GCTCAGCAGC 
GATGGTTGGA TCTGCGAGGA TGGTGACTTT GTGATGGTCC ATGCCGCCTA TACCACCCAT 
CTCTCCTCCG ATGTCTTCTT TGCGCCCGAA TCCCGTCTGG ACGATGGCCT CATCTACCTG 
GTGATCATCC GGAGAGGCGT TAGTCGCCAT CAGCTGCTCA ATTTCATGCT GAACCTAAAC 
GCAGGCACCC ATCTGCCCAT CGGCGAGGAT CCGTTCATCA AGGTGGTGCC TTGTCGGGCA 
TTCCGCATCG AGCCGAGCAG CTCCGATGGC ATCCTGGTGG TGGACGGCGA GCGGGTGGAA 
TATGGACCCA TTCAGGCGGA GGTTATGCCC GGCCTGATCA ATGTGATGAC CACCAGTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sk1 ( NM_132489.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572717.1 Drosophila melanogaster sphingosine kinase 1, transcript variant A (Sk1), mRNA.

Price & Availability↑

CloneID	OFa03515
Clone ID Related Accession (Same CDS sequence)	NM_167286.2 , NM_132489.2
Accession Version	NM_132489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sphingosine kinase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132489.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGGCCA ACACAGGGAC GTCGGGTGAA AATCTGATGG GAAATGGTGG AAAGACGCAT 
GAACCCTCCA CGCCCACTTC GGATACGGAA GTGGCCAGCG GCACGCCAAC GGAACTGAGC 
GAAATCTTCT TCGTGGACAA TAGCCGGCGC AAGCAGAGCA TCAAAATCCA GGTGAAACTA 
TGCCCGGAAG GCGTTTACCT GCGACGCGAA ACCGAGGAAG ATGATCACAT CAATGAGCAG 
CTGATCAGGA TCGATGATAT CATAGGATCA CGCTACGGAC GGCGTTTGAA GAAACGAGCC 
CGAGGCGGTC TAAACTCCTG CCGCAATCCA AATGTTCCGG GCCAGGAGGC GGATTCGGAA 
CCGGATAGCG ATAATAGCGC CTATTTGTAC ATCTATGCAT ATTTGAAGAA GGAGAAACCG 
TTGCGACGTG TCCAAACGCT CCGGATTCTA CGCTTTCGTT CGAGCAATGA CTACGGAGTG 
AATCTACAGA CCGCCGAGAT GTGGCATCAT ACGATTCGAA AGCACAAGCG TGGCAATGGC 
AGCAGTTCGC CCGCCGATTG TGGCAAACAG TTGCTCATCC TACTGAATCC GAAATCCGGT 
TCGGGCAAAG GGCGTGAGCT CTTCCAGAAA CAGGTGGCAC CTTTGCTGAC GGAAGCAGAG 
GTGCAATACG ATCTCCAGAT CACCACACAT CCGCAGTATG CCAAGGAGTT CGTGCGGACC 
AGAAGGGATC TGCTGACACG CTATTCGGGC ATTGTGGTTG CCTCCGGCGA TGGTCTATTC 
TACGAAGTGC TCAATGGGCT AATGGAACGC ATGGATTGGC GCCGAGCCTG CAGGGAGCTA 
CCGCTTGGCA TTATACCATG TGGTTCCGGG AATGGTCTGG CCAAAAGTGT GGCCCATCAT 
TGCAATGAAC CGTACGAACC GAAGCCCATT CTCCACGCCA CCTTGACCTG CATGGCGGGC 
AAAAGTACAC CCATGGATGT GGTCAGAGTG GAGCTGGCGA CGCGGGACAA GCACTTTGTG 
ATGTACTCCT TCCTGTCGGT GGGCTGGGGT CTGATAGCCG ACATCGATAT AGAGAGCGAG 
CGATTGAGAT CGATTGGAGC GCAAAGGTTT ACGCTGTGGG CCATCAAGCG ATTGATCGGG 
CTGCGCAGCT ACAAAGGCCG AGTGTCCTAT CTACTGGGCA AGGGCAAGAA GGAACCACCA 
GTGGAAGCGG CTCGAGAGTT GCCTGCAGAA TCAACGGCTG CAGGAATCCG CTCATCTCTG 
CCTCTGAATG CCGGGGAATT CCATGATCTA CCCGAGGAGG AGGAGGGGGA GGCGGTCTTG 
GATGGAGAAC AGTTCGCCGA TGCCATATCT TTGGATCGTT CGGTTTACCG CCAGCATGCC 
GACAGTTGGC ACTCGGCCAT GTCCAGGCGA ACGGCATATT ACTCCCTGGG CGGACCCAGT 
ATGCGATCCA ATCGCAGCCG GATGAGCATT AGCCAGCGGA TCGAGGCAGC AAATGCGGAA 
TTCGCTGAGA GGGTGCCAAC GGGCACCATT CCACCATTAC AGATGCCACT GCTCAGCAGC 
GATGGTTGGA TCTGCGAGGA TGGTGACTTT GTGATGGTCC ATGCCGCCTA TACCACCCAT 
CTCTCCTCCG ATGTCTTCTT TGCGCCCGAA TCCCGTCTGG ACGATGGCCT CATCTACCTG 
GTGATCATCC GGAGAGGCGT TAGTCGCCAT CAGCTGCTCA ATTTCATGCT GAACCTAAAC 
GCAGGCACCC ATCTGCCCAT CGGCGAGGAT CCGTTCATCA AGGTGGTGCC TTGTCGGGCA 
TTCCGCATCG AGCCGAGCAG CTCCGATGGC ATCCTGGTGG TGGACGGCGA GCGGGTGGAA 
TATGGACCCA TTCAGGCGGA GGTTATGCCC GGCCTGATCA ATGTGATGAC CACCAGTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572717.1
CDS	303..2228
Translation	MTANTGTSGENLMGNGGKTHEPSTPTSDTEVASGTPTELSEIFFVDNSRRKQSIKIQVKLCPEGVYLRRETEEDDHINEQLIRIDDIIGSRYGRRLKKRARGGLNSCRNPNVPGQEADSEPDSDNSAYLYIYAYLKKEKPLRRVQTLRILRFRSSNDYGVNLQTAEMWHHTIRKHKRGNGSSSPADCGKQLLILLNPKSGSGKGRELFQKQVAPLLTEAEVQYDLQITTHPQYAKEFVRTRRDLLTRYSGIVVASGDGLFYEVLNGLMERMDWRRACRELPLGIIPCGSGNGLAKSVAHHCNEPYEPKPILHATLTCMAGKSTPMDVVRVELATRDKHFVMYSFLSVGWGLIADIDIESERLRSIGAQRFTLWAIKRLIGLRSYKGRVSYLLGKGKKEPPVEAARELPAESTAAGIRSSLPLNAGEFHDLPEEEEGEAVLDGEQFADAISLDRSVYRQHADSWHSAMSRRTAYYSLGGPSMRSNRSRMSISQRIEAANAEFAERVPTGTIPPLQMPLLSSDGWICEDGDFVMVHAAYTTHLSSDVFFAPESRLDDGLIYLVIIRRGVSRHQLLNFMLNLNAGTHLPIGEDPFIKVVPCRAFRIEPSSSDGILVVDGERVEYGPIQAEVMPGLINVMTTSGQ

Target ORF information:

RefSeq Version	NM_132489.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sphingosine kinase 1, transcript variant A (Sk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132489.2

ORF Insert Sequence:

ATGACGGCCA ACACAGGGAC GTCGGGTGAA AATCTGATGG GAAATGGTGG AAAGACGCAT 
GAACCCTCCA CGCCCACTTC GGATACGGAA GTGGCCAGCG GCACGCCAAC GGAACTGAGC 
GAAATCTTCT TCGTGGACAA TAGCCGGCGC AAGCAGAGCA TCAAAATCCA GGTGAAACTA 
TGCCCGGAAG GCGTTTACCT GCGACGCGAA ACCGAGGAAG ATGATCACAT CAATGAGCAG 
CTGATCAGGA TCGATGATAT CATAGGATCA CGCTACGGAC GGCGTTTGAA GAAACGAGCC 
CGAGGCGGTC TAAACTCCTG CCGCAATCCA AATGTTCCGG GCCAGGAGGC GGATTCGGAA 
CCGGATAGCG ATAATAGCGC CTATTTGTAC ATCTATGCAT ATTTGAAGAA GGAGAAACCG 
TTGCGACGTG TCCAAACGCT CCGGATTCTA CGCTTTCGTT CGAGCAATGA CTACGGAGTG 
AATCTACAGA CCGCCGAGAT GTGGCATCAT ACGATTCGAA AGCACAAGCG TGGCAATGGC 
AGCAGTTCGC CCGCCGATTG TGGCAAACAG TTGCTCATCC TACTGAATCC GAAATCCGGT 
TCGGGCAAAG GGCGTGAGCT CTTCCAGAAA CAGGTGGCAC CTTTGCTGAC GGAAGCAGAG 
GTGCAATACG ATCTCCAGAT CACCACACAT CCGCAGTATG CCAAGGAGTT CGTGCGGACC 
AGAAGGGATC TGCTGACACG CTATTCGGGC ATTGTGGTTG CCTCCGGCGA TGGTCTATTC 
TACGAAGTGC TCAATGGGCT AATGGAACGC ATGGATTGGC GCCGAGCCTG CAGGGAGCTA 
CCGCTTGGCA TTATACCATG TGGTTCCGGG AATGGTCTGG CCAAAAGTGT GGCCCATCAT 
TGCAATGAAC CGTACGAACC GAAGCCCATT CTCCACGCCA CCTTGACCTG CATGGCGGGC 
AAAAGTACAC CCATGGATGT GGTCAGAGTG GAGCTGGCGA CGCGGGACAA GCACTTTGTG 
ATGTACTCCT TCCTGTCGGT GGGCTGGGGT CTGATAGCCG ACATCGATAT AGAGAGCGAG 
CGATTGAGAT CGATTGGAGC GCAAAGGTTT ACGCTGTGGG CCATCAAGCG ATTGATCGGG 
CTGCGCAGCT ACAAAGGCCG AGTGTCCTAT CTACTGGGCA AGGGCAAGAA GGAACCACCA 
GTGGAAGCGG CTCGAGAGTT GCCTGCAGAA TCAACGGCTG CAGGAATCCG CTCATCTCTG 
CCTCTGAATG CCGGGGAATT CCATGATCTA CCCGAGGAGG AGGAGGGGGA GGCGGTCTTG 
GATGGAGAAC AGTTCGCCGA TGCCATATCT TTGGATCGTT CGGTTTACCG CCAGCATGCC 
GACAGTTGGC ACTCGGCCAT GTCCAGGCGA ACGGCATATT ACTCCCTGGG CGGACCCAGT 
ATGCGATCCA ATCGCAGCCG GATGAGCATT AGCCAGCGGA TCGAGGCAGC AAATGCGGAA 
TTCGCTGAGA GGGTGCCAAC GGGCACCATT CCACCATTAC AGATGCCACT GCTCAGCAGC 
GATGGTTGGA TCTGCGAGGA TGGTGACTTT GTGATGGTCC ATGCCGCCTA TACCACCCAT 
CTCTCCTCCG ATGTCTTCTT TGCGCCCGAA TCCCGTCTGG ACGATGGCCT CATCTACCTG 
GTGATCATCC GGAGAGGCGT TAGTCGCCAT CAGCTGCTCA ATTTCATGCT GAACCTAAAC 
GCAGGCACCC ATCTGCCCAT CGGCGAGGAT CCGTTCATCA AGGTGGTGCC TTGTCGGGCA 
TTCCGCATCG AGCCGAGCAG CTCCGATGGC ATCCTGGTGG TGGACGGCGA GCGGGTGGAA 
TATGGACCCA TTCAGGCGGA GGTTATGCCC GGCCTGATCA ATGTGATGAC CACCAGTGGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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