Evi5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Evi5
Entrez Gene ID	32088
Full Name	Ecotropic viral integration site 5
Synonyms	BcDNA:GH08923,BcDNA:LD11130,CG11727,Dmel\CG11727,MRE21
General protein information	Preferred Names Ecotropic viral integration site 5 Names CG11727 gene product from transcript CG11727-RA CG11727-PA CG11727-PB CG11727-PC CG11727-PD CG11727-PE Evi5-PA Evi5-PB Evi5-PC Evi5-PD Evi5-PE ecotropic viral integration site 5 ecotropic viral integration site 5 ortholog mRNA-like ncRNA in embryogenesis 21
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10B11-10B14

mRNA and Protein(s)

mRNA	Protein	Name
NM_167285.4	NP_727526.2	ecotropic viral integration site 5, isoform A
NM_132488.5	NP_572716.3	ecotropic viral integration site 5, isoform B
NM_001272521.1	NP_001259450.1	ecotropic viral integration site 5, isoform E
NM_001169250.2	NP_001162721.1	ecotropic viral integration site 5, isoform C
NM_001169251.4	NP_001162722.1	ecotropic viral integration site 5, isoform D

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Evi5	NP_031990.2
Oryza sativa (rice)	Os08g0412600	NP_001061795.1
Pan troglodytes (chimpanzee)	EVI5	XP_001153700.2
	EVI5	XP_001099693.2
Canis lupus familiaris (dog)	EVI5	XP_005621976.1
Gallus gallus (chicken)	EVI5	XP_422340.3
Danio rerio (zebrafish)	evi5b	XP_003198846.2
Arabidopsis thaliana (thale cress)	PAM1	NP_197097.1
Homo sapiens (human)	EVI5	NP_005656.4
Arabidopsis thaliana (thale cress)	AT3G02460	NP_566172.1
Bos taurus (cattle)	EVI5	XP_005204382.1
Rattus norvegicus (Norway rat)	Evi5	NP_001258339.1
Drosophila melanogaster (fruit fly)	Evi5	NP_727526.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002354	XP_312605.5
Caenorhabditis elegans (roundworm)	tbc-4	NP_510667.2
Xenopus tropicalis (tropical clawed frog)	evi5	XP_002933848.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Evi5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Evi5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01280	NM_001169251.4 Latest version!	Drosophila melanogaster ecotropic viral integration site 5, transcript variant D (Evi5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01281	NM_001272521.1 Latest version!	Drosophila melanogaster ecotropic viral integration site 5, transcript variant E (Evi5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01281	NM_001169250.2 Latest version!	Drosophila melanogaster ecotropic viral integration site 5, transcript variant C (Evi5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03513	NM_167285.4 Latest version!	Drosophila melanogaster ecotropic viral integration site 5, transcript variant A (Evi5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03514	NM_132488.5 Latest version!	Drosophila melanogaster ecotropic viral integration site 5, transcript variant B (Evi5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Evi5 ( NM_001169251.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162722.1 Drosophila melanogaster ecotropic viral integration site 5, transcript variant D (Evi5), mRNA.

Price & Availability↑

CloneID	OFa01280
Clone ID Related Accession (Same CDS sequence)	NM_001169251.4
Accession Version	NM_001169251.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecotropic viral integration site 5, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169251.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAATAACT CCCGGCAATC GTCCATTGAC GAGCTCTGCA TGAAGGAGGA GGCGCTGAAG 
CAGCGCGACG AGATGGTCTC CTGTTTGCTG GAGGAACTGG TGAAGGTGCG TCAGGGACTG 
GCCGAAAGCG AGGATCAGAT CAGGAACCTC AAGGCCAAGG TCGAGGAGCT GGAGGAGGAT 
AAGAAGACGC TGCGGGAAAC GACGCCAGAC AATTCGGTGG CTCATCTGCA GGATGAACTG 
ATTGCTAGTA AACTTCGCGA GGCTGAGGCC TCACTTTCGC TAAAGGATCT CAAGCAGAGG 
GTGCAGGAGC TGAGCAGCCA GTGGCAGCGT CAATTGGCCG AGAATCAGCG CAGCGAAAGC 
GAACGCACCA CCAACGCTGT GGACTCTACG CCCAAAAAGC TGTTGACCAA CTTCTTTGAC 
AGCTCCAAGA GTAGCGAGCA CACGCAGAAG CTCGAGGAGG AGCTGATGAC CACGCGCATA 
CGCGAGATGG AGACGCTTAC CGAGCTCAAG GAGCTGCGAC TTAAGGTCAT GGAGCTGGAG 
ACGCAGGTCC AGGTGTCCAC CAATCAGCTG AGGCGGCAGG ACGAGGAGCA CAAGAAGCTC 
AAGGAGGAGC TCGAAATGGC CGTGACCCGG GAGAAGGACA TGTCCAACAA GGCGCGTGAA 
CAGCAACACA GATACTCGGA TCTGGAATCT CGCATGAAGG ATGAGCTGAT GAATGTGAAA 
ATCAAATTCA CCGAGCAAAG CCAAACGGTG GCTGAACTGA AACAGGAAAT TTCCCGTCTT 
GAGACCAAGA ACTCGGAAAT GCTCGCCGAG GGCGAACTGC GCGCGAATCT GGATGATTCG 
GACAAGGTGC GCGACCTGCA GGACAGGCTG GCCGACATGA AGGCGGAGCT GACGGCATTG 
AAAAGTCGTG GCAAATTTCC GGGCGCCAAA CTGCGCAGCT CCTCGATCCA ATCGATCGAA 
TCGACCGAGA TCGACTTCAA TGATCTGAAT ATGGTGAGGC GCGGAAGTAC GGAGCTCTCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162722.1
CDS	804..3209
Translation	MTLTTTTTASSAESQAKMDVKGGALPGEENLPTSEMDLLAKLEAANKLIESDAKSLNSLHSTHSRKNSDTSQISLTSSGNSVAEEDIWTTWATILNDWEGALKRKNPCVSELVRRGIPHHFRAIVWQQLSGASDGDKKQYAEYIKATSACEKVIRRDIARTYPEVEFFKEKDGPGQEALFNVIKAYSLHDREVGYCQGSGFIVGLLLMQMPEEEAFAVLVQIMQQHRMRHMFKPSMSELGLCMYQLENLVQEQIPDMHIHFQQQGFQTTMYASSWFLTLYTTTLNVNLSCRIMDVFLSEGMEFIFKVALALLLTGKDTLLCLDMEAMLKFFQKELPGRVEADVEGFFNLAYSIKLNTKRMKKMEKEYQDLKKKEQEEMAELRRLRRENCLLKQRNELLEAESAELADRLVRGQVSRAEEEETSYAIQTELMQLRRSYLEVSHQLENANEEVRGLSLRLQENNNSRQSSIDELCMKEEALKQRDEMVSCLLEELVKVRQGLAESEDQIRNLKAKVEELEEDKKTLRETTPDNSVAHLQDELIASKLREAEASLSLKDLKQRVQELSSQWQRQLAENQRSESERTTNAVDSTPKKLLTNFFDSSKSSEHTQKLEEELMTTRIREMETLTELKELRLKVMELETQVQVSTNQLRRQDEEHKKLKEELEMAVTREKDMSNKAREQQHRYSDLESRMKDELMNVKIKFTEQSQTVAELKQEISRLETKNSEMLAEGELRANLDDSDKVRDLQDRLADMKAELTALKSRGKFPGAKLRSSSIQSIESTEIDFNDLNMVRRGSTELST

Target ORF information:

RefSeq Version	NM_001169251.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecotropic viral integration site 5, transcript variant D (Evi5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169251.4

ORF Insert Sequence:

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAATAACT CCCGGCAATC GTCCATTGAC GAGCTCTGCA TGAAGGAGGA GGCGCTGAAG 
CAGCGCGACG AGATGGTCTC CTGTTTGCTG GAGGAACTGG TGAAGGTGCG TCAGGGACTG 
GCCGAAAGCG AGGATCAGAT CAGGAACCTC AAGGCCAAGG TCGAGGAGCT GGAGGAGGAT 
AAGAAGACGC TGCGGGAAAC GACGCCAGAC AATTCGGTGG CTCATCTGCA GGATGAACTG 
ATTGCTAGTA AACTTCGCGA GGCTGAGGCC TCACTTTCGC TAAAGGATCT CAAGCAGAGG 
GTGCAGGAGC TGAGCAGCCA GTGGCAGCGT CAATTGGCCG AGAATCAGCG CAGCGAAAGC 
GAACGCACCA CCAACGCTGT GGACTCTACG CCCAAAAAGC TGTTGACCAA CTTCTTTGAC 
AGCTCCAAGA GTAGCGAGCA CACGCAGAAG CTCGAGGAGG AGCTGATGAC CACGCGCATA 
CGCGAGATGG AGACGCTTAC CGAGCTCAAG GAGCTGCGAC TTAAGGTCAT GGAGCTGGAG 
ACGCAGGTCC AGGTGTCCAC CAATCAGCTG AGGCGGCAGG ACGAGGAGCA CAAGAAGCTC 
AAGGAGGAGC TCGAAATGGC CGTGACCCGG GAGAAGGACA TGTCCAACAA GGCGCGTGAA 
CAGCAACACA GATACTCGGA TCTGGAATCT CGCATGAAGG ATGAGCTGAT GAATGTGAAA 
ATCAAATTCA CCGAGCAAAG CCAAACGGTG GCTGAACTGA AACAGGAAAT TTCCCGTCTT 
GAGACCAAGA ACTCGGAAAT GCTCGCCGAG GGCGAACTGC GCGCGAATCT GGATGATTCG 
GACAAGGTGC GCGACCTGCA GGACAGGCTG GCCGACATGA AGGCGGAGCT GACGGCATTG 
AAAAGTCGTG GCAAATTTCC GGGCGCCAAA CTGCGCAGCT CCTCGATCCA ATCGATCGAA 
TCGACCGAGA TCGACTTCAA TGATCTGAAT ATGGTGAGGC GCGGAAGTAC GGAGCTCTCC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evi5 ( NM_001272521.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259450.1 Drosophila melanogaster ecotropic viral integration site 5, transcript variant E (Evi5), mRNA.

Price & Availability↑

CloneID	OFa01281
Clone ID Related Accession (Same CDS sequence)	NM_001272521.1 , NM_001169250.2
Accession Version	NM_001272521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecotropic viral integration site 5, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAATAACT CCCGGCAATC GTCCATTGAC GAGCTCTGCA TGAAGGAGGA GGCGCTGAAG 
CAGCGCGACG AGATGGTCTC CTGTTTGCTG GAGGAACTGG TGAAGGTGCG TCAGGGACTG 
GCCGAAAGCG AGGATCAGAT CAGGAACCTC AAGGCCAAGG TCGAGGAGCT GGAGGAGGAT 
AAGAAGACGC TGCGGGAAAC GACGCCAGAC AATTCGGTGG CTCATCTGCA GGATGAACTG 
ATTGCTAGTA AACTTCGCGA GGCTGAGGCC TCACTTTCGC TAAAGGATCT CAAGCAGAGG 
GTGCAGGAGC TGAGCAGCCA GTGGCAGCGT CAATTGGCCG AGAATCAGCG CAGCGAAAGC 
GAACGCACCA CCAACGCTGT GGACTCTACG CCCAAAAAGC TGTTGACCAA CTTCTTTGAC 
AGCTCCAAGA GTAGCGAGCA CACGCAGAAG CTCGAGGAGG AGCTGATGAC CACGCGCATA 
CGCGAGATGG AGACGCTTAC CGAGCTCAAG GAGCTGCGAC TTAAGGTCAT GGAGCTGGAG 
ACGCAGGTCC AGGTGTCCAC CAATCAGCTG AGGCGGCAGG ACGAGGAGCA CAAGAAGCTC 
AAGGAGGAGC TCGAAATGGC CGTGACCCGG GAGAAGGACA TGTCCAACAA GGCGCGTGAA 
CAGCAACACA GATACTCGGA TCTGGAATCT CGCATGAAGG ATGAGCTGAT GAATGTGAAA 
ATCAAATTCA CCGAGCAAAG CCAAACGGTG GCTGAACTGA AACAGGAAAT TTCCCGTCTT 
GAGACCAAGA ACTCGGAAAT GCTCGCCGAG GGCGAACTGC GCGCGAATCT GGATGATTCG 
GACAAGGTGC GCGACCTGCA GGACAGGCTG GCCGACATGA AGGCGGAGTA TCCCACGCCA 
ATAACCAGCC CCGACACTGA GCCGTGGAAA TGGATAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259450.1
CDS	756..3077
Translation	MTLTTTTTASSAESQAKMDVKGGALPGEENLPTSEMDLLAKLEAANKLIESDAKSLNSLHSTHSRKNSDTSQISLTSSGNSVAEEDIWTTWATILNDWEGALKRKNPCVSELVRRGIPHHFRAIVWQQLSGASDGDKKQYAEYIKATSACEKVIRRDIARTYPEVEFFKEKDGPGQEALFNVIKAYSLHDREVGYCQGSGFIVGLLLMQMPEEEAFAVLVQIMQQHRMRHMFKPSMSELGLCMYQLENLVQEQIPDMHIHFQQQGFQTTMYASSWFLTLYTTTLNVNLSCRIMDVFLSEGMEFIFKVALALLLTGKDTLLCLDMEAMLKFFQKELPGRVEADVEGFFNLAYSIKLNTKRMKKMEKEYQDLKKKEQEEMAELRRLRRENCLLKQRNELLEAESAELADRLVRGQVSRAEEEETSYAIQTELMQLRRSYLEVSHQLENANEEVRGLSLRLQENNNSRQSSIDELCMKEEALKQRDEMVSCLLEELVKVRQGLAESEDQIRNLKAKVEELEEDKKTLRETTPDNSVAHLQDELIASKLREAEASLSLKDLKQRVQELSSQWQRQLAENQRSESERTTNAVDSTPKKLLTNFFDSSKSSEHTQKLEEELMTTRIREMETLTELKELRLKVMELETQVQVSTNQLRRQDEEHKKLKEELEMAVTREKDMSNKAREQQHRYSDLESRMKDELMNVKIKFTEQSQTVAELKQEISRLETKNSEMLAEGELRANLDDSDKVRDLQDRLADMKAEYPTPITSPDTEPWKWIS

Target ORF information:

RefSeq Version	NM_001272521.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecotropic viral integration site 5, transcript variant E (Evi5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272521.1

ORF Insert Sequence:

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAATAACT CCCGGCAATC GTCCATTGAC GAGCTCTGCA TGAAGGAGGA GGCGCTGAAG 
CAGCGCGACG AGATGGTCTC CTGTTTGCTG GAGGAACTGG TGAAGGTGCG TCAGGGACTG 
GCCGAAAGCG AGGATCAGAT CAGGAACCTC AAGGCCAAGG TCGAGGAGCT GGAGGAGGAT 
AAGAAGACGC TGCGGGAAAC GACGCCAGAC AATTCGGTGG CTCATCTGCA GGATGAACTG 
ATTGCTAGTA AACTTCGCGA GGCTGAGGCC TCACTTTCGC TAAAGGATCT CAAGCAGAGG 
GTGCAGGAGC TGAGCAGCCA GTGGCAGCGT CAATTGGCCG AGAATCAGCG CAGCGAAAGC 
GAACGCACCA CCAACGCTGT GGACTCTACG CCCAAAAAGC TGTTGACCAA CTTCTTTGAC 
AGCTCCAAGA GTAGCGAGCA CACGCAGAAG CTCGAGGAGG AGCTGATGAC CACGCGCATA 
CGCGAGATGG AGACGCTTAC CGAGCTCAAG GAGCTGCGAC TTAAGGTCAT GGAGCTGGAG 
ACGCAGGTCC AGGTGTCCAC CAATCAGCTG AGGCGGCAGG ACGAGGAGCA CAAGAAGCTC 
AAGGAGGAGC TCGAAATGGC CGTGACCCGG GAGAAGGACA TGTCCAACAA GGCGCGTGAA 
CAGCAACACA GATACTCGGA TCTGGAATCT CGCATGAAGG ATGAGCTGAT GAATGTGAAA 
ATCAAATTCA CCGAGCAAAG CCAAACGGTG GCTGAACTGA AACAGGAAAT TTCCCGTCTT 
GAGACCAAGA ACTCGGAAAT GCTCGCCGAG GGCGAACTGC GCGCGAATCT GGATGATTCG 
GACAAGGTGC GCGACCTGCA GGACAGGCTG GCCGACATGA AGGCGGAGTA TCCCACGCCA 
ATAACCAGCC CCGACACTGA GCCGTGGAAA TGGATAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evi5 ( NM_001169250.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162721.1 Drosophila melanogaster ecotropic viral integration site 5, transcript variant C (Evi5), mRNA.

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CloneID	OFa01281
Clone ID Related Accession (Same CDS sequence)	NM_001272521.1 , NM_001169250.2
Accession Version	NM_001169250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecotropic viral integration site 5, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169250.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAATAACT CCCGGCAATC GTCCATTGAC GAGCTCTGCA TGAAGGAGGA GGCGCTGAAG 
CAGCGCGACG AGATGGTCTC CTGTTTGCTG GAGGAACTGG TGAAGGTGCG TCAGGGACTG 
GCCGAAAGCG AGGATCAGAT CAGGAACCTC AAGGCCAAGG TCGAGGAGCT GGAGGAGGAT 
AAGAAGACGC TGCGGGAAAC GACGCCAGAC AATTCGGTGG CTCATCTGCA GGATGAACTG 
ATTGCTAGTA AACTTCGCGA GGCTGAGGCC TCACTTTCGC TAAAGGATCT CAAGCAGAGG 
GTGCAGGAGC TGAGCAGCCA GTGGCAGCGT CAATTGGCCG AGAATCAGCG CAGCGAAAGC 
GAACGCACCA CCAACGCTGT GGACTCTACG CCCAAAAAGC TGTTGACCAA CTTCTTTGAC 
AGCTCCAAGA GTAGCGAGCA CACGCAGAAG CTCGAGGAGG AGCTGATGAC CACGCGCATA 
CGCGAGATGG AGACGCTTAC CGAGCTCAAG GAGCTGCGAC TTAAGGTCAT GGAGCTGGAG 
ACGCAGGTCC AGGTGTCCAC CAATCAGCTG AGGCGGCAGG ACGAGGAGCA CAAGAAGCTC 
AAGGAGGAGC TCGAAATGGC CGTGACCCGG GAGAAGGACA TGTCCAACAA GGCGCGTGAA 
CAGCAACACA GATACTCGGA TCTGGAATCT CGCATGAAGG ATGAGCTGAT GAATGTGAAA 
ATCAAATTCA CCGAGCAAAG CCAAACGGTG GCTGAACTGA AACAGGAAAT TTCCCGTCTT 
GAGACCAAGA ACTCGGAAAT GCTCGCCGAG GGCGAACTGC GCGCGAATCT GGATGATTCG 
GACAAGGTGC GCGACCTGCA GGACAGGCTG GCCGACATGA AGGCGGAGTA TCCCACGCCA 
ATAACCAGCC CCGACACTGA GCCGTGGAAA TGGATAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162721.1
CDS	756..3077
Translation	MTLTTTTTASSAESQAKMDVKGGALPGEENLPTSEMDLLAKLEAANKLIESDAKSLNSLHSTHSRKNSDTSQISLTSSGNSVAEEDIWTTWATILNDWEGALKRKNPCVSELVRRGIPHHFRAIVWQQLSGASDGDKKQYAEYIKATSACEKVIRRDIARTYPEVEFFKEKDGPGQEALFNVIKAYSLHDREVGYCQGSGFIVGLLLMQMPEEEAFAVLVQIMQQHRMRHMFKPSMSELGLCMYQLENLVQEQIPDMHIHFQQQGFQTTMYASSWFLTLYTTTLNVNLSCRIMDVFLSEGMEFIFKVALALLLTGKDTLLCLDMEAMLKFFQKELPGRVEADVEGFFNLAYSIKLNTKRMKKMEKEYQDLKKKEQEEMAELRRLRRENCLLKQRNELLEAESAELADRLVRGQVSRAEEEETSYAIQTELMQLRRSYLEVSHQLENANEEVRGLSLRLQENNNSRQSSIDELCMKEEALKQRDEMVSCLLEELVKVRQGLAESEDQIRNLKAKVEELEEDKKTLRETTPDNSVAHLQDELIASKLREAEASLSLKDLKQRVQELSSQWQRQLAENQRSESERTTNAVDSTPKKLLTNFFDSSKSSEHTQKLEEELMTTRIREMETLTELKELRLKVMELETQVQVSTNQLRRQDEEHKKLKEELEMAVTREKDMSNKAREQQHRYSDLESRMKDELMNVKIKFTEQSQTVAELKQEISRLETKNSEMLAEGELRANLDDSDKVRDLQDRLADMKAEYPTPITSPDTEPWKWIS

Target ORF information:

RefSeq Version	NM_001169250.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecotropic viral integration site 5, transcript variant C (Evi5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169250.2

ORF Insert Sequence:

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAATAACT CCCGGCAATC GTCCATTGAC GAGCTCTGCA TGAAGGAGGA GGCGCTGAAG 
CAGCGCGACG AGATGGTCTC CTGTTTGCTG GAGGAACTGG TGAAGGTGCG TCAGGGACTG 
GCCGAAAGCG AGGATCAGAT CAGGAACCTC AAGGCCAAGG TCGAGGAGCT GGAGGAGGAT 
AAGAAGACGC TGCGGGAAAC GACGCCAGAC AATTCGGTGG CTCATCTGCA GGATGAACTG 
ATTGCTAGTA AACTTCGCGA GGCTGAGGCC TCACTTTCGC TAAAGGATCT CAAGCAGAGG 
GTGCAGGAGC TGAGCAGCCA GTGGCAGCGT CAATTGGCCG AGAATCAGCG CAGCGAAAGC 
GAACGCACCA CCAACGCTGT GGACTCTACG CCCAAAAAGC TGTTGACCAA CTTCTTTGAC 
AGCTCCAAGA GTAGCGAGCA CACGCAGAAG CTCGAGGAGG AGCTGATGAC CACGCGCATA 
CGCGAGATGG AGACGCTTAC CGAGCTCAAG GAGCTGCGAC TTAAGGTCAT GGAGCTGGAG 
ACGCAGGTCC AGGTGTCCAC CAATCAGCTG AGGCGGCAGG ACGAGGAGCA CAAGAAGCTC 
AAGGAGGAGC TCGAAATGGC CGTGACCCGG GAGAAGGACA TGTCCAACAA GGCGCGTGAA 
CAGCAACACA GATACTCGGA TCTGGAATCT CGCATGAAGG ATGAGCTGAT GAATGTGAAA 
ATCAAATTCA CCGAGCAAAG CCAAACGGTG GCTGAACTGA AACAGGAAAT TTCCCGTCTT 
GAGACCAAGA ACTCGGAAAT GCTCGCCGAG GGCGAACTGC GCGCGAATCT GGATGATTCG 
GACAAGGTGC GCGACCTGCA GGACAGGCTG GCCGACATGA AGGCGGAGTA TCCCACGCCA 
ATAACCAGCC CCGACACTGA GCCGTGGAAA TGGATAAGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evi5 ( NM_167285.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727526.2 Drosophila melanogaster ecotropic viral integration site 5, transcript variant A (Evi5), mRNA.

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CloneID	OFa03513
Clone ID Related Accession (Same CDS sequence)	NM_167285.4
Accession Version	NM_167285.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecotropic viral integration site 5, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167285.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAACGTAT CCATCGACAG CAATAACTCC CGGCAATCGT CCATTGACGA GCTCTGCATG 
AAGGAGGAGG CGCTGAAGCA GCGCGACGAG ATGGTCTCCT GTTTGCTGGA GGAACTGGTG 
AAGGTGCGTC AGGGACTGGC CGAAAGCGAG GATCAGATCA GGAACCTCAA GGCCAAGGTC 
GAGGAGCTGG AGGAGGATAA GAAGACGCTG CGGGAAACGA CGCCAGACAA TTCGGTGGCT 
CATCTGCAGG ATGAACTGAT TGCTAGTAAA CTTCGCGAGG CTGAGGCCTC ACTTTCGCTA 
AAGGATCTCA AGCAGAGGGT GCAGGAGCTG AGCAGCCAGT GGCAGCGTCA ATTGGCCGAG 
AATCAGCGCA GCGAAAGCGA ACGCACCACC AACGCTGTGG ACTCTACGCC CAAAAAGCTG 
TTGACCAACT TCTTTGACAG CTCCAAGAGT AGCGAGCACA CGCAGAAGCT CGAGGAGGAG 
CTGATGACCA CGCGCATACG CGAGATGGAG ACGCTTACCG AGCTCAAGGA GCTGCGACTT 
AAGGTCATGG AGCTGGAGAC GCAGGTCCAG GTGTCCACCA ATCAGCTGAG GCGGCAGGAC 
GAGGAGCACA AGAAGCTCAA GGAGGAGCTC GAAATGGCCG TGACCCGGGA GAAGGACATG 
TCCAACAAGG CGCGTGAACA GCAACACAGA TACTCGGATC TGGAATCTCG CATGAAGGAT 
GAGCTGATGA ATGTGAAAAT CAAATTCACC GAGCAAAGCC AAACGGTGGC TGAACTGAAA 
CAGGAAATTT CCCGTCTTGA GACCAAGAAC TCGGAAATGC TCGCCGAGGG CGAACTGCGC 
GCGAATCTGG ATGATTCGGA CAAGGTGCGC GACCTGCAGG ACAGGCTGGC CGACATGAAG 
GCGGAGCTGA CGGCATTGAA AAGTCGTGGC AAATTTCCGG GCGCCAAACT GCGCAGCTCC 
TCGATCCAAT CGATCGAATC GACCGAGATC GACTTCAATG ATCTGAATAT GGTGAGGCGC 
GGAAGTACGG AGCTCTCCAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727526.2
CDS	756..3179
Translation	MTLTTTTTASSAESQAKMDVKGGALPGEENLPTSEMDLLAKLEAANKLIESDAKSLNSLHSTHSRKNSDTSQISLTSSGNSVAEEDIWTTWATILNDWEGALKRKNPCVSELVRRGIPHHFRAIVWQQLSGASDGDKKQYAEYIKATSACEKVIRRDIARTYPEVEFFKEKDGPGQEALFNVIKAYSLHDREVGYCQGSGFIVGLLLMQMPEEEAFAVLVQIMQQHRMRHMFKPSMSELGLCMYQLENLVQEQIPDMHIHFQQQGFQTTMYASSWFLTLYTTTLNVNLSCRIMDVFLSEGMEFIFKVALALLLTGKDTLLCLDMEAMLKFFQKELPGRVEADVEGFFNLAYSIKLNTKRMKKMEKEYQDLKKKEQEEMAELRRLRRENCLLKQRNELLEAESAELADRLVRGQVSRAEEEETSYAIQTELMQLRRSYLEVSHQLENANEEVRGLSLRLQENNVSIDSNNSRQSSIDELCMKEEALKQRDEMVSCLLEELVKVRQGLAESEDQIRNLKAKVEELEEDKKTLRETTPDNSVAHLQDELIASKLREAEASLSLKDLKQRVQELSSQWQRQLAENQRSESERTTNAVDSTPKKLLTNFFDSSKSSEHTQKLEEELMTTRIREMETLTELKELRLKVMELETQVQVSTNQLRRQDEEHKKLKEELEMAVTREKDMSNKAREQQHRYSDLESRMKDELMNVKIKFTEQSQTVAELKQEISRLETKNSEMLAEGELRANLDDSDKVRDLQDRLADMKAELTALKSRGKFPGAKLRSSSIQSIESTEIDFNDLNMVRRGSTELST

Target ORF information:

RefSeq Version	NM_167285.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecotropic viral integration site 5, transcript variant A (Evi5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167285.4

ORF Insert Sequence:

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAACGTAT CCATCGACAG CAATAACTCC CGGCAATCGT CCATTGACGA GCTCTGCATG 
AAGGAGGAGG CGCTGAAGCA GCGCGACGAG ATGGTCTCCT GTTTGCTGGA GGAACTGGTG 
AAGGTGCGTC AGGGACTGGC CGAAAGCGAG GATCAGATCA GGAACCTCAA GGCCAAGGTC 
GAGGAGCTGG AGGAGGATAA GAAGACGCTG CGGGAAACGA CGCCAGACAA TTCGGTGGCT 
CATCTGCAGG ATGAACTGAT TGCTAGTAAA CTTCGCGAGG CTGAGGCCTC ACTTTCGCTA 
AAGGATCTCA AGCAGAGGGT GCAGGAGCTG AGCAGCCAGT GGCAGCGTCA ATTGGCCGAG 
AATCAGCGCA GCGAAAGCGA ACGCACCACC AACGCTGTGG ACTCTACGCC CAAAAAGCTG 
TTGACCAACT TCTTTGACAG CTCCAAGAGT AGCGAGCACA CGCAGAAGCT CGAGGAGGAG 
CTGATGACCA CGCGCATACG CGAGATGGAG ACGCTTACCG AGCTCAAGGA GCTGCGACTT 
AAGGTCATGG AGCTGGAGAC GCAGGTCCAG GTGTCCACCA ATCAGCTGAG GCGGCAGGAC 
GAGGAGCACA AGAAGCTCAA GGAGGAGCTC GAAATGGCCG TGACCCGGGA GAAGGACATG 
TCCAACAAGG CGCGTGAACA GCAACACAGA TACTCGGATC TGGAATCTCG CATGAAGGAT 
GAGCTGATGA ATGTGAAAAT CAAATTCACC GAGCAAAGCC AAACGGTGGC TGAACTGAAA 
CAGGAAATTT CCCGTCTTGA GACCAAGAAC TCGGAAATGC TCGCCGAGGG CGAACTGCGC 
GCGAATCTGG ATGATTCGGA CAAGGTGCGC GACCTGCAGG ACAGGCTGGC CGACATGAAG 
GCGGAGCTGA CGGCATTGAA AAGTCGTGGC AAATTTCCGG GCGCCAAACT GCGCAGCTCC 
TCGATCCAAT CGATCGAATC GACCGAGATC GACTTCAATG ATCTGAATAT GGTGAGGCGC 
GGAAGTACGG AGCTCTCCAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evi5 ( NM_132488.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572716.3 Drosophila melanogaster ecotropic viral integration site 5, transcript variant B (Evi5), mRNA.

Price & Availability↑

CloneID	OFa03514
Clone ID Related Accession (Same CDS sequence)	NM_132488.5
Accession Version	NM_132488.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ecotropic viral integration site 5, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132488.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAACGTAT CCATCGACAG CAATAACTCC CGGCAATCGT CCATTGACGA GCTCTGCATG 
AAGGAGGAGG CGCTGAAGCA GCGCGACGAG ATGGTCTCCT GTTTGCTGGA GGAACTGGTG 
AAGGTGCGTC AGGGACTGGC CGAAAGCGAG GATCAGATCA GGAACCTCAA GGCCAAGGTC 
GAGGAGCTGG AGGAGGATAA GAAGACGCTG CGGGAAACGA CGCCAGACAA TTCGGTGGCT 
CATCTGCAGG ATGAACTGAT TGCTAGTAAA CTTCGCGAGG CTGAGGCCTC ACTTTCGCTA 
AAGGATCTCA AGCAGAGGGT GCAGGAGCTG AGCAGCCAGT GGCAGCGTCA ATTGGCCGAG 
AATCAGCGCA GCGAAAGCGA ACGCACCACC AACGCTGTGG ACTCTACGCC CAAAAAGCTG 
TTGACCAACT TCTTTGACAG CTCCAAGAGT AGCGAGCACA CGCAGAAGCT CGAGGAGGAG 
CTGATGACCA CGCGCATACG CGAGATGGAG ACGCTTACCG AGCTCAAGGA GCTGCGACTT 
AAGGTCATGG AGCTGGAGAC GCAGGTCCAG GTGTCCACCA ATCAGCTGAG GCGGCAGGAC 
GAGGAGCACA AGAAGCTCAA GGAGGAGCTC GAAATGGCCG TGACCCGGGA GAAGGACATG 
TCCAACAAGG CGCGTGAACA GCAACACAGA TACTCGGATC TGGAATCTCG CATGAAGGAT 
GAGCTGATGA ATGTGAAAAT CAAATTCACC GAGCAAAGCC AAACGGTGGC TGAACTGAAA 
CAGGAAATTT CCCGTCTTGA GACCAAGAAC TCGGAAATGC TCGCCGAGGG CGAACTGCGC 
GCGAATCTGG ATGATTCGGA CAAGGTGCGC GACCTGCAGG ACAGGCTGGC CGACATGAAG 
GCGGAGTATC CCACGCCAAT AACCAGCCCC GACACTGAGC CGTGGAAATG GATAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572716.3
CDS	756..3095
Translation	MTLTTTTTASSAESQAKMDVKGGALPGEENLPTSEMDLLAKLEAANKLIESDAKSLNSLHSTHSRKNSDTSQISLTSSGNSVAEEDIWTTWATILNDWEGALKRKNPCVSELVRRGIPHHFRAIVWQQLSGASDGDKKQYAEYIKATSACEKVIRRDIARTYPEVEFFKEKDGPGQEALFNVIKAYSLHDREVGYCQGSGFIVGLLLMQMPEEEAFAVLVQIMQQHRMRHMFKPSMSELGLCMYQLENLVQEQIPDMHIHFQQQGFQTTMYASSWFLTLYTTTLNVNLSCRIMDVFLSEGMEFIFKVALALLLTGKDTLLCLDMEAMLKFFQKELPGRVEADVEGFFNLAYSIKLNTKRMKKMEKEYQDLKKKEQEEMAELRRLRRENCLLKQRNELLEAESAELADRLVRGQVSRAEEEETSYAIQTELMQLRRSYLEVSHQLENANEEVRGLSLRLQENNVSIDSNNSRQSSIDELCMKEEALKQRDEMVSCLLEELVKVRQGLAESEDQIRNLKAKVEELEEDKKTLRETTPDNSVAHLQDELIASKLREAEASLSLKDLKQRVQELSSQWQRQLAENQRSESERTTNAVDSTPKKLLTNFFDSSKSSEHTQKLEEELMTTRIREMETLTELKELRLKVMELETQVQVSTNQLRRQDEEHKKLKEELEMAVTREKDMSNKAREQQHRYSDLESRMKDELMNVKIKFTEQSQTVAELKQEISRLETKNSEMLAEGELRANLDDSDKVRDLQDRLADMKAEYPTPITSPDTEPWKWIS

Target ORF information:

RefSeq Version	NM_132488.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ecotropic viral integration site 5, transcript variant B (Evi5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132488.5

ORF Insert Sequence:

ATGACCCTGA CCACAACGAC CACTGCCTCA TCAGCCGAGA GCCAAGCGAA AATGGACGTA 
AAGGGCGGCG CACTTCCCGG CGAAGAGAAC CTGCCCACAT CCGAAATGGA TCTGCTGGCC 
AAACTGGAGG CGGCCAATAA GCTCATCGAG AGCGATGCCA AGTCCCTCAA CTCTCTGCAT 
TCCACGCACA GTCGCAAGAA CTCGGATACG AGTCAAATCA GTCTTACGTC GAGTGGAAAT 
TCGGTGGCCG AAGAGGACAT CTGGACGACG TGGGCCACCA TCCTCAATGA CTGGGAGGGA 
GCACTGAAAC GAAAGAATCC CTGCGTCAGT GAGCTGGTGC GCCGTGGAAT TCCACACCAC 
TTCCGAGCGA TCGTATGGCA GCAGCTAAGT GGAGCGTCCG ACGGCGACAA AAAACAGTAT 
GCGGAGTACA TCAAGGCGAC CAGTGCCTGC GAGAAGGTCA TACGGCGTGA CATTGCCCGC 
ACCTATCCCG AGGTGGAGTT CTTCAAGGAG AAGGATGGAC CCGGACAGGA GGCCCTGTTC 
AATGTGATCA AGGCATATTC TCTGCACGAT CGCGAGGTCG GCTACTGCCA GGGCTCGGGA 
TTCATTGTGG GCCTGCTGCT GATGCAAATG CCCGAGGAGG AGGCATTCGC TGTGCTCGTG 
CAGATCATGC AGCAGCATCG GATGCGGCAC ATGTTTAAGC CCTCCATGTC GGAGTTGGGC 
TTGTGCATGT ACCAGCTGGA GAATCTGGTG CAGGAGCAAA TACCCGACAT GCACATTCAC 
TTCCAGCAGC AGGGCTTCCA GACGACCATG TACGCCTCCA GTTGGTTCCT CACCCTATAC 
ACGACCACTC TGAATGTCAA TCTAAGTTGT CGCATCATGG ACGTCTTCCT CAGCGAGGGC 
ATGGAGTTCA TCTTTAAGGT GGCCTTGGCC CTGCTGCTCA CCGGCAAGGA TACGTTGCTC 
TGCCTGGACA TGGAGGCCAT GCTTAAGTTC TTCCAAAAGG AGCTGCCCGG TCGCGTGGAG 
GCGGATGTCG AGGGCTTCTT CAATCTAGCC TACTCCATAA AGCTGAATAC TAAGCGTATG 
AAGAAGATGG AAAAGGAGTA CCAGGACCTC AAGAAGAAGG AGCAGGAGGA GATGGCCGAG 
CTGCGGCGAC TGAGACGCGA GAACTGCCTG CTGAAGCAGC GCAACGAGCT GCTGGAGGCC 
GAGAGTGCCG AACTGGCCGA TCGCCTGGTC CGTGGTCAGG TCTCCCGAGC TGAGGAGGAG 
GAAACTAGCT ATGCCATTCA GACGGAGCTA ATGCAGCTGC GCCGATCATA CTTGGAGGTC 
TCGCATCAGC TGGAAAACGC CAACGAGGAG GTGCGCGGCC TGAGTCTACG GCTGCAGGAG 
AATAACGTAT CCATCGACAG CAATAACTCC CGGCAATCGT CCATTGACGA GCTCTGCATG 
AAGGAGGAGG CGCTGAAGCA GCGCGACGAG ATGGTCTCCT GTTTGCTGGA GGAACTGGTG 
AAGGTGCGTC AGGGACTGGC CGAAAGCGAG GATCAGATCA GGAACCTCAA GGCCAAGGTC 
GAGGAGCTGG AGGAGGATAA GAAGACGCTG CGGGAAACGA CGCCAGACAA TTCGGTGGCT 
CATCTGCAGG ATGAACTGAT TGCTAGTAAA CTTCGCGAGG CTGAGGCCTC ACTTTCGCTA 
AAGGATCTCA AGCAGAGGGT GCAGGAGCTG AGCAGCCAGT GGCAGCGTCA ATTGGCCGAG 
AATCAGCGCA GCGAAAGCGA ACGCACCACC AACGCTGTGG ACTCTACGCC CAAAAAGCTG 
TTGACCAACT TCTTTGACAG CTCCAAGAGT AGCGAGCACA CGCAGAAGCT CGAGGAGGAG 
CTGATGACCA CGCGCATACG CGAGATGGAG ACGCTTACCG AGCTCAAGGA GCTGCGACTT 
AAGGTCATGG AGCTGGAGAC GCAGGTCCAG GTGTCCACCA ATCAGCTGAG GCGGCAGGAC 
GAGGAGCACA AGAAGCTCAA GGAGGAGCTC GAAATGGCCG TGACCCGGGA GAAGGACATG 
TCCAACAAGG CGCGTGAACA GCAACACAGA TACTCGGATC TGGAATCTCG CATGAAGGAT 
GAGCTGATGA ATGTGAAAAT CAAATTCACC GAGCAAAGCC AAACGGTGGC TGAACTGAAA 
CAGGAAATTT CCCGTCTTGA GACCAAGAAC TCGGAAATGC TCGCCGAGGG CGAACTGCGC 
GCGAATCTGG ATGATTCGGA CAAGGTGCGC GACCTGCAGG ACAGGCTGGC CGACATGAAG 
GCGGAGTATC CCACGCCAAT AACCAGCCCC GACACTGAGC CGTGGAAATG GATAAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Evi5 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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