sev cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sev
Entrez Gene ID	32039
Full Name	sevenless
Synonyms	7LES,7less,CG18085,CT3980,DROSEV,DROSEV1,DmHD-265,Dmel\CG18085,HD-265,SEV,Sev,sev(AC)[[14]]
General protein information	Preferred Names sevenless Names CG18085 gene product from transcript CG18085-RA CG18085-PA sev-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 10A4-10A4\|1-33.4 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078559.3	NP_511114.2	sevenless

Pathways from BioSystems

Homologies

Homo sapiens (human)	ROS1	NP_002935.2
	ROS1	XP_001110859.2
Xenopus tropicalis (tropical clawed frog)	ros1	XP_004914923.1
Gallus gallus (chicken)	ROS1	NP_990588.2
Canis lupus familiaris (dog)	ROS1	XP_541215.4
Rattus norvegicus (Norway rat)	Ros1	NP_037006.1
Danio rerio (zebrafish)	ros1	XP_694329.6
Pan troglodytes (chimpanzee)	ROS1	XP_527487.2
Drosophila melanogaster (fruit fly)	sev	NP_511114.2
Bos taurus (cattle)	ROS1	XP_003586512.2
Mus musculus (house mouse)	Ros1	NP_035412.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000015	XP_566417.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of Usp5 results in upregulation of Notch signaling and downregulation of RTK signaling by EGF receptor (EGFR) and Sevenless (Sev), leading to impaired photoreceptor development.
Title: The deubiquitinating enzyme Usp5 regulates Notch and RTK signaling during Drosophila eye development.

We show that Rap1 activity induces and/or maintains the formation of the apical domain of developing photoreceptor cells and that this event correlates with the apical accumulation and activity of SEV in presumptive R7 cells.
Title: Apical accumulation of the Sevenless receptor tyrosine kinase during Drosophila eye development is promoted by the small GTPase Rap1.

Possibility of conserved Sevenless signaling controlled patterning processes in endopterygote insects.
Title: Fast co-evolution of sevenless and bride of sevenless in endopterygote insects.

Sevenless (Sev), is required to ensure that the niche develops in the anterior region of the male embryonic gonads
Title: Boss/Sev signaling from germline to soma restricts germline-stem-cell-niche formation in the anterior region of Drosophila male gonads.

The following sev gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sev cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03464	NM_078559.3 Latest version!	Drosophila melanogaster sevenless (sev), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sev ( NM_078559.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511114.2 Drosophila melanogaster sevenless (sev), mRNA.

Price & Availability↑

CloneID	OFa03464
Clone ID Related Accession (Same CDS sequence)	NM_078559.3
Accession Version	NM_078559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sevenless
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078559.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTATGT TTTGGCAACA AAATGTAGAC CACCAGTCGG ATGAGCAGGA CAAACAGGCG 
AAGGGTGCGG CGCCCACAAA GAGATTGAAC ATCAGCTTCA ATGTGAAGAT CGCGGTGAAT 
GTGAATACCA AGATGACCAC CACCCACATC AATCAGCAGG CACCTGGCAC CTCCTCCTCC 
TCGTCGAACT CCCAGAATGC CTCACCCAGC AAGATTGTGG TCCGCCAGCA GAGCAGCTCC 
TTCGATCTAC GTCAGCAATT GGCTCGCTTA GGCCGCCAAT TGGCCAGTGG CCAGGATGGC 
CATGGCGGCA TATCCACCAT ACTGATCATC AATCTTCTCC TGCTCATTCT ACTCTCGATC 
TGCTGCGATG TCTGCCGATC GCACAACTAT ACGGTGCACC AGAGTCCCGA ACCCGTCTCC 
AAAGACCAAA TGCGCCTGCT GCGTCCCAAG CTGGACAGCG ATGTGGTCGA GAAGGTGGCC 
ATCTGGCACA AGCACGCCGC CGCAGCTCCG CCAAGCATTG TCGAGGGCAT CGCCATCAGC 
AGCAGGCCAC AGTCAACGAT GGCCCATCAT CCAGATGATC GAGATCGGGA TCGTGATCCT 
TCGGAGGAAC AGCATGGCGT CGACGAGCGA ATGGTCCTGG AACGCGTGAC CAGGGATTGT 
GTGCAGCGCT GCATTGTTGA GGAGGATCTG TTTCTGGACG AATTTGGAAT ACAGTGCGAA 
AAGGCGGACA ATGGCGAGAA GTGCTACAAA ACACGATGCA CCAAGGGCTG TGCCCAGTGG 
TATCGCGCCC TCAAGGAGCT GGAGTCCTGC CAGGAGGCCT GCCTGTCCCT CCAGTTCTAC 
CCGTACGACA TGCCCTGCAT CGGTGCCTGC GAGATGGCCC AGCGGGACTA CTGGCACCTC 
CAGCGACTGG CCATCAGCCA CCTGGTGGAG CGGACGCAGC CGCAGCTGGA GCGAGCTCCT 
CGGGCGGACG GACAGTCCAC GCCACTGACC ATCCGCTGGG CGATGCACTT TCCGGAACAC 
TACCTGGCCA GCAGACCCTT CAACATTCAG TACCAGTTTG TGGATCACCA CGGCGAGGAG 
CTGGATCTCG AGCAGGAAGA CCAGGATGCA TCCGGAGAGA CGGGTTCCAG TGCCTGGTTT 
AACTTAGCCG ATTACGACTG TGACGAGTAT TATGTGTGCG AGATACTGGA GGCCCTGATA 
CCCTACACAC AATACAGGTT CCGATTTGAG TTGCCTTTCG GCGAGAATAG AGACGAAGTC 
CTCTACTCTC CGGCCACGCC CGCCTACCAA ACGCCACCCG AGGGCGCGCC CATCTCGGCT 
CCGGTCATCG AGCATCTGAT GGGTCTCGAC GACAGCCACC TGGCTGTCCA CTGGCATCCC 
GGTCGCTTCA CCAATGGACC CATCGAGGGT TACCGCCTGC GTTTGAGTTC CTCGGAGGGA 
AACGCTACAA GTGAACAGCT GGTTCCGGCC GGACGAGGTA GCTACATCTT TTCCCAGCTA 
CAAGCCGGCA CCAACTATAC CCTGGCGCTG AGCATGATCA ACAAACAGGG TGAGGGTCCG 
GTGGCCAAGG GATTTGTGCA GACTCACTCC GCTCGAAATG AAAAGCCTGC CAAGGATCTG 
ACAGAAAGTG TCCTGCTCGT CGGACGAAGG GCTGTGATGT GGCAATCGCT GGAACCGGCT 
GGTGAGAACT CCATGATCTA TCAATCTCAG GAGGAATTGG CTGATATCGC CTGGTCAAAG 
CGGGAGCAAC AACTGTGGCT GCTCAACGTC CATGGAGAGT TGCGCAGCTT AAAATTTGAA 
TCCGGCCAGA TGGTGAGTCC AGCGCAGCAG CTCAAGCTGG ATCTGGGAAA CATATCCAGT 
GGAAGATGGG TTCCTCGCAG ATTGAGCTTC GACTGGCTGC ATCATCGACT GTACTTCGCT 
ATGGAGTCGC CAGAGCGAAA CCAATCCAGC TTTCAGATTA TCAGCACAGA TTTGCTGGGT 
GAATCAACGC AGAAAGTGGG CGAGTCCTTT GATCTGCCCG TTGAGCAGTT GGAAGTGGAT 
GCCCTGAATG GCTGGATTTT CTGGAGGAAC GAGGAGTCGC TGTGGCGTCA GGATCTGCAT 
GGTCGAATGA TCCATCGCCT GTTGAGGATC AGGCAGCCCG GTTGGTTCCT GGTGCAGCCA 
CAACACTTCA TCATCCATCT AATGCTTCCA CAGGAGGGTA AATTCCTAGA GATAAGCTAC 
GATGGTGGGT TCAAGCATCC ACTGCCGCTA CCACCGCCTT CGAATGGAGC TGGAAATGGA 
CCTGCATCCA GCCATTGGCA AAGCTTTGCC CTGTTGGGTC GCTCCCTGCT CCTGCCCGAT 
TCTGGTCAGC TGATCCTGGT TGAGCAGCAG GGTCAGGCAG CCAGTCCCAG TGCCTCATGG 
CCTCTAAAGA ACTTGCCCGA CTGTTGGGCC GTGATACTCC TGGTGCCGGA AAGCCAACCA 
CTGACCAGCG CTGGAGGAAA ACCGCACAGC TTGAAGGCCT TGCTGGGAGC CCAGGCGGCA 
AAGATCTCGT GGAAGGAGCC GGAACGCAAT CCCTACCAAT CGGCGGATGC AGCACGCAGC 
TGGAGCTACG AACTGGAAGT GCTTGATGTG GCCAGCCAAA GTGCCTTTAG CATTCGCAAT 
ATTCGTGGAC CCATCTTTGG ACTGCAGCGC CTGCAGCCGG ATAATCTCTA TCAACTGCGA 
GTTAGGGCAA TAAACGTGGA TGGAGAGCCG GGCGAGTGGA CTGAACCGTT GGCTGCCCGC 
ACCTGGCCAC TGGGTCCACA TCGCTTGAGA TGGGCCAGCC GGCAGGGAAG CGTCATTCAT 
ACCAACGAGC TGGGCGAGGG CTTGGAAGTG CAGCAGGAAC AGTTGGAGCG ACTACCCGGA 
CCCATGACCA TGGTGAATGA AAGCGTGGGC TACTACGTCA CTGGCGACGG TCTACTGCAC 
TGCATCAATC TGGTGCACAG CCAGTGGGGA TGCCCAATCT CGGAGCCACT GCAGCACGTG 
GGCTCGGTGA CTTACGACTG GCGGGGCGGA AGAGTTTATT GGACGGATCT GGCCAGGAAT 
TGCGTGGTGC GCATGGATCC ATGGTCGGGC AGTCGGGAAC TGTTGCCCGT CTTCGAGGCC 
AACTTCCTGG CATTGGATCC GCGTCAAGGC CACCTGTACT ATGCCACCAG CTCTCAGCTG 
TCGCGACATG GTTCCACGCC CGATGAAGCG GTCACTTATT ATCGTGTTAA TGGGCTGGAG 
GGAAGCATCG CCTCCTTTGT GCTGGACACC CAGCAGGATC AGCTCTTCTG GCTTGTTAAA 
GGCTCTGGTG CACTGCGTTT GTATCGTGCG CCCCTGACAG CTGGCGGGGA TTCACTGCAG 
ATGATCCAGC AGATTAAAGG CGTCTTTCAG GCTGTCCCGG ACAGTTTGCA GCTTCTGCGG 
CCCTTGGGCG CACTTCTTTG GCTGGAGCGG AGTGGCAGGA GAGCTCGCTT GGTCCGCCTG 
GCTGCTCCTC TGGATGTCAT GGAGCTACCG ACACCGGACC AGGCCTCTCC TGCCTCCGCA 
TTGCAATTAT TGGACCCACA ACCATTGCCT CCGCGGGATG AGGGGGTTAT TCCAATGACC 
GTGCTCCCGG ATAGCGTGCG TCTGGACGAT GGCCACTGGG ACGACTTCCA TGTGCGCTGG 
CAGCCATCCA CTTCCGGTGG CAATCACAGC GTCTCCTATC GTCTGCTCCT CGAGTTTGGC 
CAAAGACTAC AAACCTTGGA TTTGAGCACA CCATTTGCCC GGCTGACCCA ATTGCCGCAG 
GCTCAATTGC AGCTAAAGAT CAGCATCACA CCGCGAACCG CGTGGCGAAG TGGAGACACC 
ACTCGGGTGC AGCTCACCAC CCCGCCGGTG GCTCCTAGTC AGCCTCGTCG TCTGCGCGTG 
TTCGTGGAGC GCTTGGCCAC TGCCCTGCAG GAGGCTAATG TGAGTGCTGT GCTCCGCTGG 
GATGCGCCGG AACAGGGTCA GGAGGCGCCG ATGCAGGCGC TGGAGTATCA CATCAGCTGT 
TGGGTGGGCT CAGAGCTGCA CGAGGAGTTG CGCCTCAATC AGAGTGCCCT GGAGGCCCGC 
GTAGAGCACC TGCAACCGGA TCAGACGTAC CACTTCCAGG TGGAGGCACG TGTGGCTGCC 
ACGGGAGCGG CAGCGGGCGC AGCTAGTCAT GCCCTCCATG TGGCACCGGA GGTGCAGGCG 
GTGCCACGCG TACTCTACGC CAATGCAGAG TTTATTGGCG AACTGGACCT GGACACACGG 
AATCGCAGGC GACTGGTGCA CACGGCCAGT CCGGTTGAGC ATCTGGTGGG GATCGAGGGA 
GAGCAGCGAT TGCTGTGGGT CAACGAGCAC GTGGAGCTGC TCACCCATGT CCCGGGATCA 
GCTCCAGCAA AGCTGGCCAG AATGAGGGCC GAGGTCTTGG CACTGGCCGT GGACTGGATA 
CAGCGTATCG TCTACTGGGC GGAACTGGAT GCTACTGCAC CGCAGGCGGC GATAATCTAT 
CGCCTGGATC TGTGCAACTT TGAAGGGAAG ATCCTGCAGG GCGAGCGGGT GTGGAGCACT 
CCCAGGGGAC GGTTGCTGAA GGATCTGGTG GCCCTGCCAC AGGCGCAATC TCTGATCTGG 
TTGGAGTACG AGCAGGGATC TCCGAGAAAT GGTTCGCTCC GGGGCAGAAA TCTAACCGAT 
GGCTCGGAGC TGGAGTGGGC AACGGTTCAG CCGCTAATCC GTCTGCATGC TGGAAGCTTA 
GAGCCCGGAT CGGAGACCTT GAACCTGGTG GACAACCAGG GCAAGCTGTG TGTCTACGAT 
GTGGCCCGTC AGCTGTGCAC GGCCAGCGCT TTGCGGGCAC AGCTGAACTT GCTGGGCGAG 
GACTCCATTG CTGGTCAGTT GGCCCAGGAT TCGGGATACC TTTACGCCGT GAAAAACTGG 
AGCATTCGTG CTTATGGTCG CCGGCGCCAG CAGCTGGAGT ATACGGTGGA ACTGGAGCCG 
GAAGAGGTGC GTCTGCTCCA GGCACACAAC TATCAGGCCT ATCCGCCCAA GAACTGTCTG 
CTCCTTCCTT CATCCGGCGG ATCCCTTTTG AAAGCTACCG ATTGTGAGGA GCAGAGATGC 
CTATTACACT TACCCATGAT CACAGCCTCC GAAGATTGTC CACTGCCTAT TCCTGGCGTT 
AGATACCAAC TGAATCTTAC GTTGGCCAAG GAGCCGGGAT CCGAGGAGCA CGATCATGGG 
ATGGAGCCCC TGGGACAGTG GCTGCTCGGT GCTGGGGAAT CGTTGAATCT TACAGACCTG 
CTGCCCTTCA CCCGTTATCG CGTGTCTGGA ATTTTGAGCA GCTTTTACCA AAAGAAGTTG 
GCATTACCCA CCTTGGTGTT GGCACCACTG GAGCTCCTTA CCGCCTCTGC CACGCCCTCG 
CCTCCAAGGA ACTTCAGTGT TCGTGTGCTT AGTCCCAGGG AACTGGAGGT CAGCTGGTTG 
CCGCCGGAGC AGCTGCGTAG CGAAAGTGTC TACTACACGC TCCACTGGCA ACAGGAACTG 
GATGGTGAAA ATGTCCAGGA TCGGCGGGAA TGGGAGGCAC ATGAGCGGCG ACTGGAGACG 
GCGGGCACTC ATCGATTGAC TGGAATCAAG CCGGGATCTG GGTATAGCCT GTGGGTTCAG 
GCCCATGCCA CGCCCACCAA GAGCAACAGC AGCGAGCGGC TGCATGTGCG TAGTTTCGCC 
GAATTACCCG AGTTGCAGCT CCTGGAACTG GGACCCTATT CTCTGAGTCT CACCTGGGCG 
GGAACACCGG ATCCACTGGG ATCGCTGCAG CTCGAATGCC GATCGTCGGC TGAGCAACTG 
CGTCGCAATG TGGCCGGAAA TCACACTAAG ATGGTGGTGG AGCCATTGCA GCCACGCACC 
CGCTACCAGT GTCGCCTGCT CCTGGGCTAT GCGGCGACGC CGGGAGCTCC ACTGTACCAT 
GGCACTGCAG AGGTGTACGA AACTCTGGGA GATGCGCCCA GTCAGCCGGG CAAGCCACAA 
TTGGAGCACA TCGCTGAGGA GGTTTTCCGT GTCACCTGGA CGGCGGCCCG CGGTAATGGA 
GCACCCATTG CTCTCTACAA TCTGGAGGCA CTCCAGGCGA GGAGCGACAT TCGCCGGAGG 
CGCAGAAGAA GGCGCCGCAA TAGCGGTGGA TCACTAGAGC AGTTGCCGTG GGCTGAGGAA 
CCGGTGGTCG TGGAGGATCA GTGGCTGGAC TTCTGCAACA CCACCGAGCT GAGCTGCATT 
GTGAAGAGTC TGCATTCGAG CAGGTTGCTC CTCTTCCGTG TGCGTGCGCG GAGCTTGGAG 
CACGGATGGG GACCTTACAG CGAGGAGAGC GAACGGGTGG CGGAGCCCTT CGTTTCGCCG 
GAGAAGAGAG GATCACTGGT CCTGGCCATC ATTGCGCCGG CTGCCATCGT TTCCAGCTGC 
GTTCTGGCAT TGGTGCTCGT TCGAAAAGTT CAAAAGCGAC GACTGCGTGC CAAGAAACTG 
CTTCAGCAGA GCCGTCCCAG CATCTGGAGC AACCTGTCCA CCTTGCAGAC GCAACAGCAG 
CTGATGGCCG TCAGGAATCG CGCCTTCTCC ACCACGCTGA GTGATGCGGA CATCGCTCTG 
CTGCCCCAAA TTAATTGGAG TCAACTGAAG TTGCTCCGAT TTCTTGGCAG CGGTGCTTTT 
GGTGAGGTAT ACGAGGGTCA GTTGAAGACG GAGGACTCCG AGGAGCCGCA ACGAGTGGCC 
ATCAAGAGCC TGCGCAAGGG AGCCAGTGAA TTTGCCGAGC TGCTTCAGGA GGCTCAGCTG 
ATGAGCAACT TCAAGCACGA GAACATTGTG TGCCTGGTGG GAATCTGCTT CGACACCGAG 
TCCATATCTC TGATTATGGA GCACATGGAA GCGGGCGACT TGCTAAGCTA CCTACGTGCC 
GCCAGGGCTA CCAGCACCCA GGAGCCACAA CCCACTGCTG GACTCTCGCT CTCCGAGCTC 
CTGGCCATGT GCATTGATGT GGCCAACGGC TGCAGTTATC TGGAGGACAT GCACTTTGTG 
CATCGCGACC TGGCCTGCCG GAACTGTTTG GTCACGGAAT CGACGGGCAG CACGGATCGT 
CGGCGCACCG TGAAGATTGG TGACTTCGGA TTGGCGAGGG ACATCTACAA GAGCGACTAC 
TACCGAAAGG AGGGCGAGGG CCTGCTACCG GTGCGCTGGA TGTCGCCGGA GAGTCTCGTC 
GACGGGTTGT TCACCACACA GTCGGATGTG TGGGCCTTTG GAGTGCTCTG CTGGGAGATC 
CTCACACTGG GCCAGCAGCC GTATGCGGCG AGGAACAACT TCGAGGTGCT GGCCCATGTC 
AAGGAGGGCG GACGGCTCCA GCAGCCGCCC ATGTGTACGG AGAAGCTTTA CTCCCTGCTC 
CTGCTTTGCT GGCGAACGGA TCCGTGGGAG CGACCCAGTT TCCGGCGCTG CTACAACACA 
CTCCATGCCA TCAGCACCGA TTTGCGGCGC ACCCAAATGG CCTCGGCAAC TGCGGATACG 
GTTGTCAGCT GCTCCAGGCC GGAGTTCAAG GTGCGATTCG ATGGCCAGCC GCTGGAGGAG 
CATAGAGAGC ACAATGAGCG GCCGGAGGAT GAGAACCTGA CGCTGCGGGA AGTGCCGCTC 
AAGGATAAGC AACTGTATGC AAACGAGGGA GTCTCGCGAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511114.2
CDS	227..7891
Translation	MTMFWQQNVDHQSDEQDKQAKGAAPTKRLNISFNVKIAVNVNTKMTTTHINQQAPGTSSSSSNSQNASPSKIVVRQQSSSFDLRQQLARLGRQLASGQDGHGGISTILIINLLLLILLSICCDVCRSHNYTVHQSPEPVSKDQMRLLRPKLDSDVVEKVAIWHKHAAAAPPSIVEGIAISSRPQSTMAHHPDDRDRDRDPSEEQHGVDERMVLERVTRDCVQRCIVEEDLFLDEFGIQCEKADNGEKCYKTRCTKGCAQWYRALKELESCQEACLSLQFYPYDMPCIGACEMAQRDYWHLQRLAISHLVERTQPQLERAPRADGQSTPLTIRWAMHFPEHYLASRPFNIQYQFVDHHGEELDLEQEDQDASGETGSSAWFNLADYDCDEYYVCEILEALIPYTQYRFRFELPFGENRDEVLYSPATPAYQTPPEGAPISAPVIEHLMGLDDSHLAVHWHPGRFTNGPIEGYRLRLSSSEGNATSEQLVPAGRGSYIFSQLQAGTNYTLALSMINKQGEGPVAKGFVQTHSARNEKPAKDLTESVLLVGRRAVMWQSLEPAGENSMIYQSQEELADIAWSKREQQLWLLNVHGELRSLKFESGQMVSPAQQLKLDLGNISSGRWVPRRLSFDWLHHRLYFAMESPERNQSSFQIISTDLLGESTQKVGESFDLPVEQLEVDALNGWIFWRNEESLWRQDLHGRMIHRLLRIRQPGWFLVQPQHFIIHLMLPQEGKFLEISYDGGFKHPLPLPPPSNGAGNGPASSHWQSFALLGRSLLLPDSGQLILVEQQGQAASPSASWPLKNLPDCWAVILLVPESQPLTSAGGKPHSLKALLGAQAAKISWKEPERNPYQSADAARSWSYELEVLDVASQSAFSIRNIRGPIFGLQRLQPDNLYQLRVRAINVDGEPGEWTEPLAARTWPLGPHRLRWASRQGSVIHTNELGEGLEVQQEQLERLPGPMTMVNESVGYYVTGDGLLHCINLVHSQWGCPISEPLQHVGSVTYDWRGGRVYWTDLARNCVVRMDPWSGSRELLPVFEANFLALDPRQGHLYYATSSQLSRHGSTPDEAVTYYRVNGLEGSIASFVLDTQQDQLFWLVKGSGALRLYRAPLTAGGDSLQMIQQIKGVFQAVPDSLQLLRPLGALLWLERSGRRARLVRLAAPLDVMELPTPDQASPASALQLLDPQPLPPRDEGVIPMTVLPDSVRLDDGHWDDFHVRWQPSTSGGNHSVSYRLLLEFGQRLQTLDLSTPFARLTQLPQAQLQLKISITPRTAWRSGDTTRVQLTTPPVAPSQPRRLRVFVERLATALQEANVSAVLRWDAPEQGQEAPMQALEYHISCWVGSELHEELRLNQSALEARVEHLQPDQTYHFQVEARVAATGAAAGAASHALHVAPEVQAVPRVLYANAEFIGELDLDTRNRRRLVHTASPVEHLVGIEGEQRLLWVNEHVELLTHVPGSAPAKLARMRAEVLALAVDWIQRIVYWAELDATAPQAAIIYRLDLCNFEGKILQGERVWSTPRGRLLKDLVALPQAQSLIWLEYEQGSPRNGSLRGRNLTDGSELEWATVQPLIRLHAGSLEPGSETLNLVDNQGKLCVYDVARQLCTASALRAQLNLLGEDSIAGQLAQDSGYLYAVKNWSIRAYGRRRQQLEYTVELEPEEVRLLQAHNYQAYPPKNCLLLPSSGGSLLKATDCEEQRCLLHLPMITASEDCPLPIPGVRYQLNLTLAKEPGSEEHDHGMEPLGQWLLGAGESLNLTDLLPFTRYRVSGILSSFYQKKLALPTLVLAPLELLTASATPSPPRNFSVRVLSPRELEVSWLPPEQLRSESVYYTLHWQQELDGENVQDRREWEAHERRLETAGTHRLTGIKPGSGYSLWVQAHATPTKSNSSERLHVRSFAELPELQLLELGPYSLSLTWAGTPDPLGSLQLECRSSAEQLRRNVAGNHTKMVVEPLQPRTRYQCRLLLGYAATPGAPLYHGTAEVYETLGDAPSQPGKPQLEHIAEEVFRVTWTAARGNGAPIALYNLEALQARSDIRRRRRRRRRNSGGSLEQLPWAEEPVVVEDQWLDFCNTTELSCIVKSLHSSRLLLFRVRARSLEHGWGPYSEESERVAEPFVSPEKRGSLVLAIIAPAAIVSSCVLALVLVRKVQKRRLRAKKLLQQSRPSIWSNLSTLQTQQQLMAVRNRAFSTTLSDADIALLPQINWSQLKLLRFLGSGAFGEVYEGQLKTEDSEEPQRVAIKSLRKGASEFAELLQEAQLMSNFKHENIVCLVGICFDTESISLIMEHMEAGDLLSYLRAARATSTQEPQPTAGLSLSELLAMCIDVANGCSYLEDMHFVHRDLACRNCLVTESTGSTDRRRTVKIGDFGLARDIYKSDYYRKEGEGLLPVRWMSPESLVDGLFTTQSDVWAFGVLCWEILTLGQQPYAARNNFEVLAHVKEGGRLQQPPMCTEKLYSLLLLCWRTDPWERPSFRRCYNTLHAISTDLRRTQMASATADTVVSCSRPEFKVRFDGQPLEEHREHNERPEDENLTLREVPLKDKQLYANEGVSRL

Target ORF information:

RefSeq Version	NM_078559.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sevenless (sev), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078559.3

ORF Insert Sequence:

ATGACTATGT TTTGGCAACA AAATGTAGAC CACCAGTCGG ATGAGCAGGA CAAACAGGCG 
AAGGGTGCGG CGCCCACAAA GAGATTGAAC ATCAGCTTCA ATGTGAAGAT CGCGGTGAAT 
GTGAATACCA AGATGACCAC CACCCACATC AATCAGCAGG CACCTGGCAC CTCCTCCTCC 
TCGTCGAACT CCCAGAATGC CTCACCCAGC AAGATTGTGG TCCGCCAGCA GAGCAGCTCC 
TTCGATCTAC GTCAGCAATT GGCTCGCTTA GGCCGCCAAT TGGCCAGTGG CCAGGATGGC 
CATGGCGGCA TATCCACCAT ACTGATCATC AATCTTCTCC TGCTCATTCT ACTCTCGATC 
TGCTGCGATG TCTGCCGATC GCACAACTAT ACGGTGCACC AGAGTCCCGA ACCCGTCTCC 
AAAGACCAAA TGCGCCTGCT GCGTCCCAAG CTGGACAGCG ATGTGGTCGA GAAGGTGGCC 
ATCTGGCACA AGCACGCCGC CGCAGCTCCG CCAAGCATTG TCGAGGGCAT CGCCATCAGC 
AGCAGGCCAC AGTCAACGAT GGCCCATCAT CCAGATGATC GAGATCGGGA TCGTGATCCT 
TCGGAGGAAC AGCATGGCGT CGACGAGCGA ATGGTCCTGG AACGCGTGAC CAGGGATTGT 
GTGCAGCGCT GCATTGTTGA GGAGGATCTG TTTCTGGACG AATTTGGAAT ACAGTGCGAA 
AAGGCGGACA ATGGCGAGAA GTGCTACAAA ACACGATGCA CCAAGGGCTG TGCCCAGTGG 
TATCGCGCCC TCAAGGAGCT GGAGTCCTGC CAGGAGGCCT GCCTGTCCCT CCAGTTCTAC 
CCGTACGACA TGCCCTGCAT CGGTGCCTGC GAGATGGCCC AGCGGGACTA CTGGCACCTC 
CAGCGACTGG CCATCAGCCA CCTGGTGGAG CGGACGCAGC CGCAGCTGGA GCGAGCTCCT 
CGGGCGGACG GACAGTCCAC GCCACTGACC ATCCGCTGGG CGATGCACTT TCCGGAACAC 
TACCTGGCCA GCAGACCCTT CAACATTCAG TACCAGTTTG TGGATCACCA CGGCGAGGAG 
CTGGATCTCG AGCAGGAAGA CCAGGATGCA TCCGGAGAGA CGGGTTCCAG TGCCTGGTTT 
AACTTAGCCG ATTACGACTG TGACGAGTAT TATGTGTGCG AGATACTGGA GGCCCTGATA 
CCCTACACAC AATACAGGTT CCGATTTGAG TTGCCTTTCG GCGAGAATAG AGACGAAGTC 
CTCTACTCTC CGGCCACGCC CGCCTACCAA ACGCCACCCG AGGGCGCGCC CATCTCGGCT 
CCGGTCATCG AGCATCTGAT GGGTCTCGAC GACAGCCACC TGGCTGTCCA CTGGCATCCC 
GGTCGCTTCA CCAATGGACC CATCGAGGGT TACCGCCTGC GTTTGAGTTC CTCGGAGGGA 
AACGCTACAA GTGAACAGCT GGTTCCGGCC GGACGAGGTA GCTACATCTT TTCCCAGCTA 
CAAGCCGGCA CCAACTATAC CCTGGCGCTG AGCATGATCA ACAAACAGGG TGAGGGTCCG 
GTGGCCAAGG GATTTGTGCA GACTCACTCC GCTCGAAATG AAAAGCCTGC CAAGGATCTG 
ACAGAAAGTG TCCTGCTCGT CGGACGAAGG GCTGTGATGT GGCAATCGCT GGAACCGGCT 
GGTGAGAACT CCATGATCTA TCAATCTCAG GAGGAATTGG CTGATATCGC CTGGTCAAAG 
CGGGAGCAAC AACTGTGGCT GCTCAACGTC CATGGAGAGT TGCGCAGCTT AAAATTTGAA 
TCCGGCCAGA TGGTGAGTCC AGCGCAGCAG CTCAAGCTGG ATCTGGGAAA CATATCCAGT 
GGAAGATGGG TTCCTCGCAG ATTGAGCTTC GACTGGCTGC ATCATCGACT GTACTTCGCT 
ATGGAGTCGC CAGAGCGAAA CCAATCCAGC TTTCAGATTA TCAGCACAGA TTTGCTGGGT 
GAATCAACGC AGAAAGTGGG CGAGTCCTTT GATCTGCCCG TTGAGCAGTT GGAAGTGGAT 
GCCCTGAATG GCTGGATTTT CTGGAGGAAC GAGGAGTCGC TGTGGCGTCA GGATCTGCAT 
GGTCGAATGA TCCATCGCCT GTTGAGGATC AGGCAGCCCG GTTGGTTCCT GGTGCAGCCA 
CAACACTTCA TCATCCATCT AATGCTTCCA CAGGAGGGTA AATTCCTAGA GATAAGCTAC 
GATGGTGGGT TCAAGCATCC ACTGCCGCTA CCACCGCCTT CGAATGGAGC TGGAAATGGA 
CCTGCATCCA GCCATTGGCA AAGCTTTGCC CTGTTGGGTC GCTCCCTGCT CCTGCCCGAT 
TCTGGTCAGC TGATCCTGGT TGAGCAGCAG GGTCAGGCAG CCAGTCCCAG TGCCTCATGG 
CCTCTAAAGA ACTTGCCCGA CTGTTGGGCC GTGATACTCC TGGTGCCGGA AAGCCAACCA 
CTGACCAGCG CTGGAGGAAA ACCGCACAGC TTGAAGGCCT TGCTGGGAGC CCAGGCGGCA 
AAGATCTCGT GGAAGGAGCC GGAACGCAAT CCCTACCAAT CGGCGGATGC AGCACGCAGC 
TGGAGCTACG AACTGGAAGT GCTTGATGTG GCCAGCCAAA GTGCCTTTAG CATTCGCAAT 
ATTCGTGGAC CCATCTTTGG ACTGCAGCGC CTGCAGCCGG ATAATCTCTA TCAACTGCGA 
GTTAGGGCAA TAAACGTGGA TGGAGAGCCG GGCGAGTGGA CTGAACCGTT GGCTGCCCGC 
ACCTGGCCAC TGGGTCCACA TCGCTTGAGA TGGGCCAGCC GGCAGGGAAG CGTCATTCAT 
ACCAACGAGC TGGGCGAGGG CTTGGAAGTG CAGCAGGAAC AGTTGGAGCG ACTACCCGGA 
CCCATGACCA TGGTGAATGA AAGCGTGGGC TACTACGTCA CTGGCGACGG TCTACTGCAC 
TGCATCAATC TGGTGCACAG CCAGTGGGGA TGCCCAATCT CGGAGCCACT GCAGCACGTG 
GGCTCGGTGA CTTACGACTG GCGGGGCGGA AGAGTTTATT GGACGGATCT GGCCAGGAAT 
TGCGTGGTGC GCATGGATCC ATGGTCGGGC AGTCGGGAAC TGTTGCCCGT CTTCGAGGCC 
AACTTCCTGG CATTGGATCC GCGTCAAGGC CACCTGTACT ATGCCACCAG CTCTCAGCTG 
TCGCGACATG GTTCCACGCC CGATGAAGCG GTCACTTATT ATCGTGTTAA TGGGCTGGAG 
GGAAGCATCG CCTCCTTTGT GCTGGACACC CAGCAGGATC AGCTCTTCTG GCTTGTTAAA 
GGCTCTGGTG CACTGCGTTT GTATCGTGCG CCCCTGACAG CTGGCGGGGA TTCACTGCAG 
ATGATCCAGC AGATTAAAGG CGTCTTTCAG GCTGTCCCGG ACAGTTTGCA GCTTCTGCGG 
CCCTTGGGCG CACTTCTTTG GCTGGAGCGG AGTGGCAGGA GAGCTCGCTT GGTCCGCCTG 
GCTGCTCCTC TGGATGTCAT GGAGCTACCG ACACCGGACC AGGCCTCTCC TGCCTCCGCA 
TTGCAATTAT TGGACCCACA ACCATTGCCT CCGCGGGATG AGGGGGTTAT TCCAATGACC 
GTGCTCCCGG ATAGCGTGCG TCTGGACGAT GGCCACTGGG ACGACTTCCA TGTGCGCTGG 
CAGCCATCCA CTTCCGGTGG CAATCACAGC GTCTCCTATC GTCTGCTCCT CGAGTTTGGC 
CAAAGACTAC AAACCTTGGA TTTGAGCACA CCATTTGCCC GGCTGACCCA ATTGCCGCAG 
GCTCAATTGC AGCTAAAGAT CAGCATCACA CCGCGAACCG CGTGGCGAAG TGGAGACACC 
ACTCGGGTGC AGCTCACCAC CCCGCCGGTG GCTCCTAGTC AGCCTCGTCG TCTGCGCGTG 
TTCGTGGAGC GCTTGGCCAC TGCCCTGCAG GAGGCTAATG TGAGTGCTGT GCTCCGCTGG 
GATGCGCCGG AACAGGGTCA GGAGGCGCCG ATGCAGGCGC TGGAGTATCA CATCAGCTGT 
TGGGTGGGCT CAGAGCTGCA CGAGGAGTTG CGCCTCAATC AGAGTGCCCT GGAGGCCCGC 
GTAGAGCACC TGCAACCGGA TCAGACGTAC CACTTCCAGG TGGAGGCACG TGTGGCTGCC 
ACGGGAGCGG CAGCGGGCGC AGCTAGTCAT GCCCTCCATG TGGCACCGGA GGTGCAGGCG 
GTGCCACGCG TACTCTACGC CAATGCAGAG TTTATTGGCG AACTGGACCT GGACACACGG 
AATCGCAGGC GACTGGTGCA CACGGCCAGT CCGGTTGAGC ATCTGGTGGG GATCGAGGGA 
GAGCAGCGAT TGCTGTGGGT CAACGAGCAC GTGGAGCTGC TCACCCATGT CCCGGGATCA 
GCTCCAGCAA AGCTGGCCAG AATGAGGGCC GAGGTCTTGG CACTGGCCGT GGACTGGATA 
CAGCGTATCG TCTACTGGGC GGAACTGGAT GCTACTGCAC CGCAGGCGGC GATAATCTAT 
CGCCTGGATC TGTGCAACTT TGAAGGGAAG ATCCTGCAGG GCGAGCGGGT GTGGAGCACT 
CCCAGGGGAC GGTTGCTGAA GGATCTGGTG GCCCTGCCAC AGGCGCAATC TCTGATCTGG 
TTGGAGTACG AGCAGGGATC TCCGAGAAAT GGTTCGCTCC GGGGCAGAAA TCTAACCGAT 
GGCTCGGAGC TGGAGTGGGC AACGGTTCAG CCGCTAATCC GTCTGCATGC TGGAAGCTTA 
GAGCCCGGAT CGGAGACCTT GAACCTGGTG GACAACCAGG GCAAGCTGTG TGTCTACGAT 
GTGGCCCGTC AGCTGTGCAC GGCCAGCGCT TTGCGGGCAC AGCTGAACTT GCTGGGCGAG 
GACTCCATTG CTGGTCAGTT GGCCCAGGAT TCGGGATACC TTTACGCCGT GAAAAACTGG 
AGCATTCGTG CTTATGGTCG CCGGCGCCAG CAGCTGGAGT ATACGGTGGA ACTGGAGCCG 
GAAGAGGTGC GTCTGCTCCA GGCACACAAC TATCAGGCCT ATCCGCCCAA GAACTGTCTG 
CTCCTTCCTT CATCCGGCGG ATCCCTTTTG AAAGCTACCG ATTGTGAGGA GCAGAGATGC 
CTATTACACT TACCCATGAT CACAGCCTCC GAAGATTGTC CACTGCCTAT TCCTGGCGTT 
AGATACCAAC TGAATCTTAC GTTGGCCAAG GAGCCGGGAT CCGAGGAGCA CGATCATGGG 
ATGGAGCCCC TGGGACAGTG GCTGCTCGGT GCTGGGGAAT CGTTGAATCT TACAGACCTG 
CTGCCCTTCA CCCGTTATCG CGTGTCTGGA ATTTTGAGCA GCTTTTACCA AAAGAAGTTG 
GCATTACCCA CCTTGGTGTT GGCACCACTG GAGCTCCTTA CCGCCTCTGC CACGCCCTCG 
CCTCCAAGGA ACTTCAGTGT TCGTGTGCTT AGTCCCAGGG AACTGGAGGT CAGCTGGTTG 
CCGCCGGAGC AGCTGCGTAG CGAAAGTGTC TACTACACGC TCCACTGGCA ACAGGAACTG 
GATGGTGAAA ATGTCCAGGA TCGGCGGGAA TGGGAGGCAC ATGAGCGGCG ACTGGAGACG 
GCGGGCACTC ATCGATTGAC TGGAATCAAG CCGGGATCTG GGTATAGCCT GTGGGTTCAG 
GCCCATGCCA CGCCCACCAA GAGCAACAGC AGCGAGCGGC TGCATGTGCG TAGTTTCGCC 
GAATTACCCG AGTTGCAGCT CCTGGAACTG GGACCCTATT CTCTGAGTCT CACCTGGGCG 
GGAACACCGG ATCCACTGGG ATCGCTGCAG CTCGAATGCC GATCGTCGGC TGAGCAACTG 
CGTCGCAATG TGGCCGGAAA TCACACTAAG ATGGTGGTGG AGCCATTGCA GCCACGCACC 
CGCTACCAGT GTCGCCTGCT CCTGGGCTAT GCGGCGACGC CGGGAGCTCC ACTGTACCAT 
GGCACTGCAG AGGTGTACGA AACTCTGGGA GATGCGCCCA GTCAGCCGGG CAAGCCACAA 
TTGGAGCACA TCGCTGAGGA GGTTTTCCGT GTCACCTGGA CGGCGGCCCG CGGTAATGGA 
GCACCCATTG CTCTCTACAA TCTGGAGGCA CTCCAGGCGA GGAGCGACAT TCGCCGGAGG 
CGCAGAAGAA GGCGCCGCAA TAGCGGTGGA TCACTAGAGC AGTTGCCGTG GGCTGAGGAA 
CCGGTGGTCG TGGAGGATCA GTGGCTGGAC TTCTGCAACA CCACCGAGCT GAGCTGCATT 
GTGAAGAGTC TGCATTCGAG CAGGTTGCTC CTCTTCCGTG TGCGTGCGCG GAGCTTGGAG 
CACGGATGGG GACCTTACAG CGAGGAGAGC GAACGGGTGG CGGAGCCCTT CGTTTCGCCG 
GAGAAGAGAG GATCACTGGT CCTGGCCATC ATTGCGCCGG CTGCCATCGT TTCCAGCTGC 
GTTCTGGCAT TGGTGCTCGT TCGAAAAGTT CAAAAGCGAC GACTGCGTGC CAAGAAACTG 
CTTCAGCAGA GCCGTCCCAG CATCTGGAGC AACCTGTCCA CCTTGCAGAC GCAACAGCAG 
CTGATGGCCG TCAGGAATCG CGCCTTCTCC ACCACGCTGA GTGATGCGGA CATCGCTCTG 
CTGCCCCAAA TTAATTGGAG TCAACTGAAG TTGCTCCGAT TTCTTGGCAG CGGTGCTTTT 
GGTGAGGTAT ACGAGGGTCA GTTGAAGACG GAGGACTCCG AGGAGCCGCA ACGAGTGGCC 
ATCAAGAGCC TGCGCAAGGG AGCCAGTGAA TTTGCCGAGC TGCTTCAGGA GGCTCAGCTG 
ATGAGCAACT TCAAGCACGA GAACATTGTG TGCCTGGTGG GAATCTGCTT CGACACCGAG 
TCCATATCTC TGATTATGGA GCACATGGAA GCGGGCGACT TGCTAAGCTA CCTACGTGCC 
GCCAGGGCTA CCAGCACCCA GGAGCCACAA CCCACTGCTG GACTCTCGCT CTCCGAGCTC 
CTGGCCATGT GCATTGATGT GGCCAACGGC TGCAGTTATC TGGAGGACAT GCACTTTGTG 
CATCGCGACC TGGCCTGCCG GAACTGTTTG GTCACGGAAT CGACGGGCAG CACGGATCGT 
CGGCGCACCG TGAAGATTGG TGACTTCGGA TTGGCGAGGG ACATCTACAA GAGCGACTAC 
TACCGAAAGG AGGGCGAGGG CCTGCTACCG GTGCGCTGGA TGTCGCCGGA GAGTCTCGTC 
GACGGGTTGT TCACCACACA GTCGGATGTG TGGGCCTTTG GAGTGCTCTG CTGGGAGATC 
CTCACACTGG GCCAGCAGCC GTATGCGGCG AGGAACAACT TCGAGGTGCT GGCCCATGTC 
AAGGAGGGCG GACGGCTCCA GCAGCCGCCC ATGTGTACGG AGAAGCTTTA CTCCCTGCTC 
CTGCTTTGCT GGCGAACGGA TCCGTGGGAG CGACCCAGTT TCCGGCGCTG CTACAACACA 
CTCCATGCCA TCAGCACCGA TTTGCGGCGC ACCCAAATGG CCTCGGCAAC TGCGGATACG 
GTTGTCAGCT GCTCCAGGCC GGAGTTCAAG GTGCGATTCG ATGGCCAGCC GCTGGAGGAG 
CATAGAGAGC ACAATGAGCG GCCGGAGGAT GAGAACCTGA CGCTGCGGGA AGTGCCGCTC 
AAGGATAAGC AACTGTATGC AAACGAGGGA GTCTCGCGAC TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sev cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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