CG2186 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG2186
Entrez Gene ID	32017
Full Name	CG2186 gene product from transcript CG2186-RB
Synonyms	Dmel\CG2186
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9F5-9F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_132432.1	NP_572660.1	uncharacterized protein, isoform A
NM_001272504.1	NP_001259433.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG2186 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG2186 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01254	NM_001272504.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2186), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01254	NM_132432.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2186), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG2186 ( NM_132432.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572660.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG2186), mRNA.

Price & Availability↑

CloneID	OFa01254
Clone ID Related Accession (Same CDS sequence)	NM_132432.1 , NM_001272504.1
Accession Version	NM_132432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA TTAACGATGA CCTGCTCAAC TACGAGCTGG GCGATGACAT CGATGACCAA 
GCTCTGCTCG GCGCCGATGA GGATGAGTTG CTACTCTCGG ACGAAGAGCT CGAAAAGGAT 
GTCACCAGCG AGGTGAAGCA GCAGATGCGC GCCGAGGCCG AGGAGTGGGC CCAGGAGCAG 
ATTAGACAGC ACGAGCGCAA GCACAAGGAG CAATTGCAGG CAGATGCGAC TGCAGCGACT 
GAAGTCACTG TTGCAGCCAC GGCCACCGCA GAGGTAGCAC CAACTTTAAG GGCGCAACCA 
GAGCTAGCTT CATCTCCTGC TCCGGATCCT GTTCCTGCGA AACCTCTAGT CGCTCAGGTG 
GAGAAACATG TTGACACAGC CCACGCGGCT GTGGCAGCTG AGCCAGCGAA GGAACTGGTG 
TCAGCCAGTC GCTCGGAATC CCCACAACAG CAAGCTAAGG AGGAGGCGCC TCCAGCTGAT 
TGCGCCGAAA AGGAGGCGAC TCAGAATAAT GGCAACAGCG AGGAACAAGC TGAGAACGCT 
GGCAGTCAGA GTGAGGCTGA ACGAGGACTG GGCTTGTGCT CGCTACTGGC CTCATCCCAA 
GAGAGCCTGC CCAGCGCCAG CTCAGCCTCT GTGATTTCAG ATCCGCGAGA GGACGACGAA 
GAGCGCGAGG AGCGCACCAA CTACAAGACG GCCAGCGAGC GATCTGACAA CAATCTGAGG 
CAGCAGCAAG AGCACGCACA CATGTCGCCC TACCAGCACC AGCAGCGGCG ACTCCACGGC 
CATGGAGATA AACCCCGCGT GGGCGGAGGT GGACGCTTCA TGCATGGCCA GCAGCAGCAG 
CAGCAGCAGC AACAGAGCCA GATGGGCAAG CCGCCGCGTG GAATTGGAAC GCGCCACCAT 
CTGCTACGCA ATCCGTCGCC AAGTTTCGGC ATACAGCAGC AGCGCATGGG CATGGCCGGG 
ATGCAACCGC CGCCACAGCG ATATGGCATG CCGGGCCAGT ACCCGGCAAC TCAGCCACCC 
AACCAGTCAC CACAACAAGC ACCGACCAAC ACACAACAGC CACACAGCGC TCCCGCCTCT 
CCCCTCCACA CTCCCACGCA TTCGCACATG ATGCACCACA GTCACAATCC GAACGGAGGC 
GTGCTGCACC ACCTGCTGCC GCATCCGCAC GAGCAGGTGC GTGTGGGTCT GCTGCAACCA 
CCGCCGCTGG CATATGCGCC CAATCCGGGT GGCTATCGCA ATGGAGCACT TCTGGGTCCG 
GGACCGGGAC CTGGTTTGCA TCAGCAACAC CAGTCGCCGC ACCAGCAACA GTCGGGCATG 
CGTCCGCCGC TGTATCGGAA TGCCCCGCCA TCGTACGGCT ACGACCAGGG ACATCCACCC 
CAGCATCCGC AGCAGTTCAT GCGGCCTCAC AACGGCTGGC GACCGCAGGG GGACAATGCG 
CGCATGCAGC GACCACCACT GCAAACGCTG CCACCGCACA TGATGCCCGG CGCACCGCCG 
AACTATGCCA ATGGCATGGG AATGCGAGGA CCGCCGCCCC AGCAACAGCA ATCGCAGCAG 
CCGCCACAGC AGCAGCCAGC ACCGCAGCAG GCGGGCAATC CCCTACAGCA GGCCGTCAAT 
CCTGGCCAGC CCCCTCAACA GCCGAATGGA CCGCCGCAGC AGCAGGGCTC ATCATCGGTG 
CCCCGGGTCA GCAAGGTGCT CATCAATCCG AATTTCAAGG GCGGCGTCGA AGCGGCTACC 
AACAAGTTCA TCAAGGAGAC ACACTTTATG CCCGCCATTA ACAGCGAGGC CGAGTTGCTG 
CGCCAGCAGG TGGAGTTCAT CAACAAGAAT CGGCAGTACA TTGAGAAGCG ACGCATGGAA 
CGCTCGCCAC CGTCGTCGGG CTCCTCCACC GGAGCCGCCT CGTCCGGCCA GGCAGGCGAA 
CGGAGGCGAA CACGTAGCCG CAGTCGCTCT CGTGGTCGCA GTTACTCTCC CGTTAAGCGC 
GTGGAAAGAG AACGCGAGCG GGAGCGGGAG CGCGAGAGGG ATCGTGAAAG GGATCGGGAG 
CGAGATCGTG AACGATATGG CGCCAATCGG GCGGCTGGAA ACCGTGGATT CCGACGGGCA 
TCGAGTCGGG ATCGAGACGA GAATCGTGGC GCAGCAAGCA CCGCCAGTGC TCAAGGAGCG 
GGCGGATCAG CATCCAGCGG AGCTGCGCCC CCCAAGCGAC GAAGGAGTGG CTCGGCCGGA 
GGAGGCACTG CCCGGAATCC ATTCTCTGGC GAGCCGCGCG GCCGACCCTC CGAAACAGGC 
AGTCGCAGTA TGCCCAGCGA CGAGGACGAG GAGACCCGCA ACTATCGCCT TGAGATTGAG 
AAGCAGAAGC AACTGCGCGA GAAAATCATG CGAGACAAGG AGCTGAAGCG TCGGCGCGCA 
GCCGAGGAGA AGCTACACGA GGAGAAACGC GCCGAGCAGC ACAACACTTC GCCAGCCCGC 
GACGACCAGG AGGTGGCCGC CGGACAAGGA TCTGCAGCGC CAACACCTGC AGCGTCAAAG 
CATCGGCCAA CCGCATCAGC CGGCGAGCGC AAGGTGATCT CGCTGAAGCG TCGCGACGAC 
CAGGATGCGA GCAGCGTACG CAGCAGGCAG GCACAGGGAT CGCAGTCCAA TGCTCAGGCA 
GGCCAAGGTC AGCAGCAGCA GCAGGCGGCG CGCAAGCAAC TAACGGCGGC GAAGATAGCG 
CCAGAGGCAA AGCGCAGTAT AACCGATCAC CTGGCGCCAT CCTCTAAGCA GCATCATCAT 
CATCATCAGC AGCATCATCA GACTGCAGCG GCGCCGGCGA GAACGGTGGT ATTGGGATCG 
GGAGCTGTGG CCGGCGGCAC ACCGCCGAAG CCGCGCGATA AGCTGCGCCT GGGCACGCCA 
AGCGACGATG AAGTGGAGCT GGACTATGAT GACGATGACC TGCTGCTGGA CGAGGAGGAT 
CGTTTGTTGG CCACGCCATC GCCGCCGCCG CAGAAGGCGA GCAGCAAAAG ATCGGCCACG 
CCGGAGCCCT TGGTGGTCAA GCGGAACCAC AAGGCGATGC GTGGCGGCAG CTCGACATCA 
GCGCCCAGCT TTAGCTCCAA CCAGCGGCGA GTGGTGCTCA AGCCGACCAG CAATGGCAGC 
AGCAGCAGCG GAGGAGATCA GCAGCAGTTG TCGCACAGCG GCAGGCAGCG GGATCGGAGG 
CGCGGTGGAG AGGATAGCGC ACTGCAGCGG AAAGGTGGAG TAGGAAGCGT TGGCGGCAGC 
AGCAGCTCGG GATCTGGCGG AGGGATCTTC GATAGGCTGG AGAAGCGTGT GGCCGTCTCA 
GGAGGAGGGA GCAGCGGTGG AGGCGGCGGG AGCAGCCACA AGCAGCGATC CAAGGCGCCC 
GCCCGCATCG TACTCAAGCA CGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572660.1
CDS	266..3652
Translation	MSDINDDLLNYELGDDIDDQALLGADEDELLLSDEELEKDVTSEVKQQMRAEAEEWAQEQIRQHERKHKEQLQADATAATEVTVAATATAEVAPTLRAQPELASSPAPDPVPAKPLVAQVEKHVDTAHAAVAAEPAKELVSASRSESPQQQAKEEAPPADCAEKEATQNNGNSEEQAENAGSQSEAERGLGLCSLLASSQESLPSASSASVISDPREDDEEREERTNYKTASERSDNNLRQQQEHAHMSPYQHQQRRLHGHGDKPRVGGGGRFMHGQQQQQQQQQSQMGKPPRGIGTRHHLLRNPSPSFGIQQQRMGMAGMQPPPQRYGMPGQYPATQPPNQSPQQAPTNTQQPHSAPASPLHTPTHSHMMHHSHNPNGGVLHHLLPHPHEQVRVGLLQPPPLAYAPNPGGYRNGALLGPGPGPGLHQQHQSPHQQQSGMRPPLYRNAPPSYGYDQGHPPQHPQQFMRPHNGWRPQGDNARMQRPPLQTLPPHMMPGAPPNYANGMGMRGPPPQQQQSQQPPQQQPAPQQAGNPLQQAVNPGQPPQQPNGPPQQQGSSSVPRVSKVLINPNFKGGVEAATNKFIKETHFMPAINSEAELLRQQVEFINKNRQYIEKRRMERSPPSSGSSTGAASSGQAGERRRTRSRSRSRGRSYSPVKRVERERERERERERDRERDRERDRERYGANRAAGNRGFRRASSRDRDENRGAASTASAQGAGGSASSGAAPPKRRRSGSAGGGTARNPFSGEPRGRPSETGSRSMPSDEDEETRNYRLEIEKQKQLREKIMRDKELKRRRAAEEKLHEEKRAEQHNTSPARDDQEVAAGQGSAAPTPAASKHRPTASAGERKVISLKRRDDQDASSVRSRQAQGSQSNAQAGQGQQQQQAARKQLTAAKIAPEAKRSITDHLAPSSKQHHHHHQQHHQTAAAPARTVVLGSGAVAGGTPPKPRDKLRLGTPSDDEVELDYDDDDLLLDEEDRLLATPSPPPQKASSKRSATPEPLVVKRNHKAMRGGSSTSAPSFSSNQRRVVLKPTSNGSSSSGGDQQQLSHSGRQRDRRRGGEDSALQRKGGVGSVGGSSSSGSGGGIFDRLEKRVAVSGGGSSGGGGGSSHKQRSKAPARIVLKHD

Target ORF information:

RefSeq Version	NM_132432.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2186), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132432.1

ORF Insert Sequence:

ATGTCGGACA TTAACGATGA CCTGCTCAAC TACGAGCTGG GCGATGACAT CGATGACCAA 
GCTCTGCTCG GCGCCGATGA GGATGAGTTG CTACTCTCGG ACGAAGAGCT CGAAAAGGAT 
GTCACCAGCG AGGTGAAGCA GCAGATGCGC GCCGAGGCCG AGGAGTGGGC CCAGGAGCAG 
ATTAGACAGC ACGAGCGCAA GCACAAGGAG CAATTGCAGG CAGATGCGAC TGCAGCGACT 
GAAGTCACTG TTGCAGCCAC GGCCACCGCA GAGGTAGCAC CAACTTTAAG GGCGCAACCA 
GAGCTAGCTT CATCTCCTGC TCCGGATCCT GTTCCTGCGA AACCTCTAGT CGCTCAGGTG 
GAGAAACATG TTGACACAGC CCACGCGGCT GTGGCAGCTG AGCCAGCGAA GGAACTGGTG 
TCAGCCAGTC GCTCGGAATC CCCACAACAG CAAGCTAAGG AGGAGGCGCC TCCAGCTGAT 
TGCGCCGAAA AGGAGGCGAC TCAGAATAAT GGCAACAGCG AGGAACAAGC TGAGAACGCT 
GGCAGTCAGA GTGAGGCTGA ACGAGGACTG GGCTTGTGCT CGCTACTGGC CTCATCCCAA 
GAGAGCCTGC CCAGCGCCAG CTCAGCCTCT GTGATTTCAG ATCCGCGAGA GGACGACGAA 
GAGCGCGAGG AGCGCACCAA CTACAAGACG GCCAGCGAGC GATCTGACAA CAATCTGAGG 
CAGCAGCAAG AGCACGCACA CATGTCGCCC TACCAGCACC AGCAGCGGCG ACTCCACGGC 
CATGGAGATA AACCCCGCGT GGGCGGAGGT GGACGCTTCA TGCATGGCCA GCAGCAGCAG 
CAGCAGCAGC AACAGAGCCA GATGGGCAAG CCGCCGCGTG GAATTGGAAC GCGCCACCAT 
CTGCTACGCA ATCCGTCGCC AAGTTTCGGC ATACAGCAGC AGCGCATGGG CATGGCCGGG 
ATGCAACCGC CGCCACAGCG ATATGGCATG CCGGGCCAGT ACCCGGCAAC TCAGCCACCC 
AACCAGTCAC CACAACAAGC ACCGACCAAC ACACAACAGC CACACAGCGC TCCCGCCTCT 
CCCCTCCACA CTCCCACGCA TTCGCACATG ATGCACCACA GTCACAATCC GAACGGAGGC 
GTGCTGCACC ACCTGCTGCC GCATCCGCAC GAGCAGGTGC GTGTGGGTCT GCTGCAACCA 
CCGCCGCTGG CATATGCGCC CAATCCGGGT GGCTATCGCA ATGGAGCACT TCTGGGTCCG 
GGACCGGGAC CTGGTTTGCA TCAGCAACAC CAGTCGCCGC ACCAGCAACA GTCGGGCATG 
CGTCCGCCGC TGTATCGGAA TGCCCCGCCA TCGTACGGCT ACGACCAGGG ACATCCACCC 
CAGCATCCGC AGCAGTTCAT GCGGCCTCAC AACGGCTGGC GACCGCAGGG GGACAATGCG 
CGCATGCAGC GACCACCACT GCAAACGCTG CCACCGCACA TGATGCCCGG CGCACCGCCG 
AACTATGCCA ATGGCATGGG AATGCGAGGA CCGCCGCCCC AGCAACAGCA ATCGCAGCAG 
CCGCCACAGC AGCAGCCAGC ACCGCAGCAG GCGGGCAATC CCCTACAGCA GGCCGTCAAT 
CCTGGCCAGC CCCCTCAACA GCCGAATGGA CCGCCGCAGC AGCAGGGCTC ATCATCGGTG 
CCCCGGGTCA GCAAGGTGCT CATCAATCCG AATTTCAAGG GCGGCGTCGA AGCGGCTACC 
AACAAGTTCA TCAAGGAGAC ACACTTTATG CCCGCCATTA ACAGCGAGGC CGAGTTGCTG 
CGCCAGCAGG TGGAGTTCAT CAACAAGAAT CGGCAGTACA TTGAGAAGCG ACGCATGGAA 
CGCTCGCCAC CGTCGTCGGG CTCCTCCACC GGAGCCGCCT CGTCCGGCCA GGCAGGCGAA 
CGGAGGCGAA CACGTAGCCG CAGTCGCTCT CGTGGTCGCA GTTACTCTCC CGTTAAGCGC 
GTGGAAAGAG AACGCGAGCG GGAGCGGGAG CGCGAGAGGG ATCGTGAAAG GGATCGGGAG 
CGAGATCGTG AACGATATGG CGCCAATCGG GCGGCTGGAA ACCGTGGATT CCGACGGGCA 
TCGAGTCGGG ATCGAGACGA GAATCGTGGC GCAGCAAGCA CCGCCAGTGC TCAAGGAGCG 
GGCGGATCAG CATCCAGCGG AGCTGCGCCC CCCAAGCGAC GAAGGAGTGG CTCGGCCGGA 
GGAGGCACTG CCCGGAATCC ATTCTCTGGC GAGCCGCGCG GCCGACCCTC CGAAACAGGC 
AGTCGCAGTA TGCCCAGCGA CGAGGACGAG GAGACCCGCA ACTATCGCCT TGAGATTGAG 
AAGCAGAAGC AACTGCGCGA GAAAATCATG CGAGACAAGG AGCTGAAGCG TCGGCGCGCA 
GCCGAGGAGA AGCTACACGA GGAGAAACGC GCCGAGCAGC ACAACACTTC GCCAGCCCGC 
GACGACCAGG AGGTGGCCGC CGGACAAGGA TCTGCAGCGC CAACACCTGC AGCGTCAAAG 
CATCGGCCAA CCGCATCAGC CGGCGAGCGC AAGGTGATCT CGCTGAAGCG TCGCGACGAC 
CAGGATGCGA GCAGCGTACG CAGCAGGCAG GCACAGGGAT CGCAGTCCAA TGCTCAGGCA 
GGCCAAGGTC AGCAGCAGCA GCAGGCGGCG CGCAAGCAAC TAACGGCGGC GAAGATAGCG 
CCAGAGGCAA AGCGCAGTAT AACCGATCAC CTGGCGCCAT CCTCTAAGCA GCATCATCAT 
CATCATCAGC AGCATCATCA GACTGCAGCG GCGCCGGCGA GAACGGTGGT ATTGGGATCG 
GGAGCTGTGG CCGGCGGCAC ACCGCCGAAG CCGCGCGATA AGCTGCGCCT GGGCACGCCA 
AGCGACGATG AAGTGGAGCT GGACTATGAT GACGATGACC TGCTGCTGGA CGAGGAGGAT 
CGTTTGTTGG CCACGCCATC GCCGCCGCCG CAGAAGGCGA GCAGCAAAAG ATCGGCCACG 
CCGGAGCCCT TGGTGGTCAA GCGGAACCAC AAGGCGATGC GTGGCGGCAG CTCGACATCA 
GCGCCCAGCT TTAGCTCCAA CCAGCGGCGA GTGGTGCTCA AGCCGACCAG CAATGGCAGC 
AGCAGCAGCG GAGGAGATCA GCAGCAGTTG TCGCACAGCG GCAGGCAGCG GGATCGGAGG 
CGCGGTGGAG AGGATAGCGC ACTGCAGCGG AAAGGTGGAG TAGGAAGCGT TGGCGGCAGC 
AGCAGCTCGG GATCTGGCGG AGGGATCTTC GATAGGCTGG AGAAGCGTGT GGCCGTCTCA 
GGAGGAGGGA GCAGCGGTGG AGGCGGCGGG AGCAGCCACA AGCAGCGATC CAAGGCGCCC 
GCCCGCATCG TACTCAAGCA CGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2186 ( NM_001272504.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259433.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG2186), mRNA.

Price & Availability↑

CloneID	OFa01254
Clone ID Related Accession (Same CDS sequence)	NM_132432.1 , NM_001272504.1
Accession Version	NM_001272504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGACA TTAACGATGA CCTGCTCAAC TACGAGCTGG GCGATGACAT CGATGACCAA 
GCTCTGCTCG GCGCCGATGA GGATGAGTTG CTACTCTCGG ACGAAGAGCT CGAAAAGGAT 
GTCACCAGCG AGGTGAAGCA GCAGATGCGC GCCGAGGCCG AGGAGTGGGC CCAGGAGCAG 
ATTAGACAGC ACGAGCGCAA GCACAAGGAG CAATTGCAGG CAGATGCGAC TGCAGCGACT 
GAAGTCACTG TTGCAGCCAC GGCCACCGCA GAGGTAGCAC CAACTTTAAG GGCGCAACCA 
GAGCTAGCTT CATCTCCTGC TCCGGATCCT GTTCCTGCGA AACCTCTAGT CGCTCAGGTG 
GAGAAACATG TTGACACAGC CCACGCGGCT GTGGCAGCTG AGCCAGCGAA GGAACTGGTG 
TCAGCCAGTC GCTCGGAATC CCCACAACAG CAAGCTAAGG AGGAGGCGCC TCCAGCTGAT 
TGCGCCGAAA AGGAGGCGAC TCAGAATAAT GGCAACAGCG AGGAACAAGC TGAGAACGCT 
GGCAGTCAGA GTGAGGCTGA ACGAGGACTG GGCTTGTGCT CGCTACTGGC CTCATCCCAA 
GAGAGCCTGC CCAGCGCCAG CTCAGCCTCT GTGATTTCAG ATCCGCGAGA GGACGACGAA 
GAGCGCGAGG AGCGCACCAA CTACAAGACG GCCAGCGAGC GATCTGACAA CAATCTGAGG 
CAGCAGCAAG AGCACGCACA CATGTCGCCC TACCAGCACC AGCAGCGGCG ACTCCACGGC 
CATGGAGATA AACCCCGCGT GGGCGGAGGT GGACGCTTCA TGCATGGCCA GCAGCAGCAG 
CAGCAGCAGC AACAGAGCCA GATGGGCAAG CCGCCGCGTG GAATTGGAAC GCGCCACCAT 
CTGCTACGCA ATCCGTCGCC AAGTTTCGGC ATACAGCAGC AGCGCATGGG CATGGCCGGG 
ATGCAACCGC CGCCACAGCG ATATGGCATG CCGGGCCAGT ACCCGGCAAC TCAGCCACCC 
AACCAGTCAC CACAACAAGC ACCGACCAAC ACACAACAGC CACACAGCGC TCCCGCCTCT 
CCCCTCCACA CTCCCACGCA TTCGCACATG ATGCACCACA GTCACAATCC GAACGGAGGC 
GTGCTGCACC ACCTGCTGCC GCATCCGCAC GAGCAGGTGC GTGTGGGTCT GCTGCAACCA 
CCGCCGCTGG CATATGCGCC CAATCCGGGT GGCTATCGCA ATGGAGCACT TCTGGGTCCG 
GGACCGGGAC CTGGTTTGCA TCAGCAACAC CAGTCGCCGC ACCAGCAACA GTCGGGCATG 
CGTCCGCCGC TGTATCGGAA TGCCCCGCCA TCGTACGGCT ACGACCAGGG ACATCCACCC 
CAGCATCCGC AGCAGTTCAT GCGGCCTCAC AACGGCTGGC GACCGCAGGG GGACAATGCG 
CGCATGCAGC GACCACCACT GCAAACGCTG CCACCGCACA TGATGCCCGG CGCACCGCCG 
AACTATGCCA ATGGCATGGG AATGCGAGGA CCGCCGCCCC AGCAACAGCA ATCGCAGCAG 
CCGCCACAGC AGCAGCCAGC ACCGCAGCAG GCGGGCAATC CCCTACAGCA GGCCGTCAAT 
CCTGGCCAGC CCCCTCAACA GCCGAATGGA CCGCCGCAGC AGCAGGGCTC ATCATCGGTG 
CCCCGGGTCA GCAAGGTGCT CATCAATCCG AATTTCAAGG GCGGCGTCGA AGCGGCTACC 
AACAAGTTCA TCAAGGAGAC ACACTTTATG CCCGCCATTA ACAGCGAGGC CGAGTTGCTG 
CGCCAGCAGG TGGAGTTCAT CAACAAGAAT CGGCAGTACA TTGAGAAGCG ACGCATGGAA 
CGCTCGCCAC CGTCGTCGGG CTCCTCCACC GGAGCCGCCT CGTCCGGCCA GGCAGGCGAA 
CGGAGGCGAA CACGTAGCCG CAGTCGCTCT CGTGGTCGCA GTTACTCTCC CGTTAAGCGC 
GTGGAAAGAG AACGCGAGCG GGAGCGGGAG CGCGAGAGGG ATCGTGAAAG GGATCGGGAG 
CGAGATCGTG AACGATATGG CGCCAATCGG GCGGCTGGAA ACCGTGGATT CCGACGGGCA 
TCGAGTCGGG ATCGAGACGA GAATCGTGGC GCAGCAAGCA CCGCCAGTGC TCAAGGAGCG 
GGCGGATCAG CATCCAGCGG AGCTGCGCCC CCCAAGCGAC GAAGGAGTGG CTCGGCCGGA 
GGAGGCACTG CCCGGAATCC ATTCTCTGGC GAGCCGCGCG GCCGACCCTC CGAAACAGGC 
AGTCGCAGTA TGCCCAGCGA CGAGGACGAG GAGACCCGCA ACTATCGCCT TGAGATTGAG 
AAGCAGAAGC AACTGCGCGA GAAAATCATG CGAGACAAGG AGCTGAAGCG TCGGCGCGCA 
GCCGAGGAGA AGCTACACGA GGAGAAACGC GCCGAGCAGC ACAACACTTC GCCAGCCCGC 
GACGACCAGG AGGTGGCCGC CGGACAAGGA TCTGCAGCGC CAACACCTGC AGCGTCAAAG 
CATCGGCCAA CCGCATCAGC CGGCGAGCGC AAGGTGATCT CGCTGAAGCG TCGCGACGAC 
CAGGATGCGA GCAGCGTACG CAGCAGGCAG GCACAGGGAT CGCAGTCCAA TGCTCAGGCA 
GGCCAAGGTC AGCAGCAGCA GCAGGCGGCG CGCAAGCAAC TAACGGCGGC GAAGATAGCG 
CCAGAGGCAA AGCGCAGTAT AACCGATCAC CTGGCGCCAT CCTCTAAGCA GCATCATCAT 
CATCATCAGC AGCATCATCA GACTGCAGCG GCGCCGGCGA GAACGGTGGT ATTGGGATCG 
GGAGCTGTGG CCGGCGGCAC ACCGCCGAAG CCGCGCGATA AGCTGCGCCT GGGCACGCCA 
AGCGACGATG AAGTGGAGCT GGACTATGAT GACGATGACC TGCTGCTGGA CGAGGAGGAT 
CGTTTGTTGG CCACGCCATC GCCGCCGCCG CAGAAGGCGA GCAGCAAAAG ATCGGCCACG 
CCGGAGCCCT TGGTGGTCAA GCGGAACCAC AAGGCGATGC GTGGCGGCAG CTCGACATCA 
GCGCCCAGCT TTAGCTCCAA CCAGCGGCGA GTGGTGCTCA AGCCGACCAG CAATGGCAGC 
AGCAGCAGCG GAGGAGATCA GCAGCAGTTG TCGCACAGCG GCAGGCAGCG GGATCGGAGG 
CGCGGTGGAG AGGATAGCGC ACTGCAGCGG AAAGGTGGAG TAGGAAGCGT TGGCGGCAGC 
AGCAGCTCGG GATCTGGCGG AGGGATCTTC GATAGGCTGG AGAAGCGTGT GGCCGTCTCA 
GGAGGAGGGA GCAGCGGTGG AGGCGGCGGG AGCAGCCACA AGCAGCGATC CAAGGCGCCC 
GCCCGCATCG TACTCAAGCA CGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259433.1
CDS	268..3654
Translation	MSDINDDLLNYELGDDIDDQALLGADEDELLLSDEELEKDVTSEVKQQMRAEAEEWAQEQIRQHERKHKEQLQADATAATEVTVAATATAEVAPTLRAQPELASSPAPDPVPAKPLVAQVEKHVDTAHAAVAAEPAKELVSASRSESPQQQAKEEAPPADCAEKEATQNNGNSEEQAENAGSQSEAERGLGLCSLLASSQESLPSASSASVISDPREDDEEREERTNYKTASERSDNNLRQQQEHAHMSPYQHQQRRLHGHGDKPRVGGGGRFMHGQQQQQQQQQSQMGKPPRGIGTRHHLLRNPSPSFGIQQQRMGMAGMQPPPQRYGMPGQYPATQPPNQSPQQAPTNTQQPHSAPASPLHTPTHSHMMHHSHNPNGGVLHHLLPHPHEQVRVGLLQPPPLAYAPNPGGYRNGALLGPGPGPGLHQQHQSPHQQQSGMRPPLYRNAPPSYGYDQGHPPQHPQQFMRPHNGWRPQGDNARMQRPPLQTLPPHMMPGAPPNYANGMGMRGPPPQQQQSQQPPQQQPAPQQAGNPLQQAVNPGQPPQQPNGPPQQQGSSSVPRVSKVLINPNFKGGVEAATNKFIKETHFMPAINSEAELLRQQVEFINKNRQYIEKRRMERSPPSSGSSTGAASSGQAGERRRTRSRSRSRGRSYSPVKRVERERERERERERDRERDRERDRERYGANRAAGNRGFRRASSRDRDENRGAASTASAQGAGGSASSGAAPPKRRRSGSAGGGTARNPFSGEPRGRPSETGSRSMPSDEDEETRNYRLEIEKQKQLREKIMRDKELKRRRAAEEKLHEEKRAEQHNTSPARDDQEVAAGQGSAAPTPAASKHRPTASAGERKVISLKRRDDQDASSVRSRQAQGSQSNAQAGQGQQQQQAARKQLTAAKIAPEAKRSITDHLAPSSKQHHHHHQQHHQTAAAPARTVVLGSGAVAGGTPPKPRDKLRLGTPSDDEVELDYDDDDLLLDEEDRLLATPSPPPQKASSKRSATPEPLVVKRNHKAMRGGSSTSAPSFSSNQRRVVLKPTSNGSSSSGGDQQQLSHSGRQRDRRRGGEDSALQRKGGVGSVGGSSSSGSGGGIFDRLEKRVAVSGGGSSGGGGGSSHKQRSKAPARIVLKHD

Target ORF information:

RefSeq Version	NM_001272504.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2186), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272504.1

ORF Insert Sequence:

ATGTCGGACA TTAACGATGA CCTGCTCAAC TACGAGCTGG GCGATGACAT CGATGACCAA 
GCTCTGCTCG GCGCCGATGA GGATGAGTTG CTACTCTCGG ACGAAGAGCT CGAAAAGGAT 
GTCACCAGCG AGGTGAAGCA GCAGATGCGC GCCGAGGCCG AGGAGTGGGC CCAGGAGCAG 
ATTAGACAGC ACGAGCGCAA GCACAAGGAG CAATTGCAGG CAGATGCGAC TGCAGCGACT 
GAAGTCACTG TTGCAGCCAC GGCCACCGCA GAGGTAGCAC CAACTTTAAG GGCGCAACCA 
GAGCTAGCTT CATCTCCTGC TCCGGATCCT GTTCCTGCGA AACCTCTAGT CGCTCAGGTG 
GAGAAACATG TTGACACAGC CCACGCGGCT GTGGCAGCTG AGCCAGCGAA GGAACTGGTG 
TCAGCCAGTC GCTCGGAATC CCCACAACAG CAAGCTAAGG AGGAGGCGCC TCCAGCTGAT 
TGCGCCGAAA AGGAGGCGAC TCAGAATAAT GGCAACAGCG AGGAACAAGC TGAGAACGCT 
GGCAGTCAGA GTGAGGCTGA ACGAGGACTG GGCTTGTGCT CGCTACTGGC CTCATCCCAA 
GAGAGCCTGC CCAGCGCCAG CTCAGCCTCT GTGATTTCAG ATCCGCGAGA GGACGACGAA 
GAGCGCGAGG AGCGCACCAA CTACAAGACG GCCAGCGAGC GATCTGACAA CAATCTGAGG 
CAGCAGCAAG AGCACGCACA CATGTCGCCC TACCAGCACC AGCAGCGGCG ACTCCACGGC 
CATGGAGATA AACCCCGCGT GGGCGGAGGT GGACGCTTCA TGCATGGCCA GCAGCAGCAG 
CAGCAGCAGC AACAGAGCCA GATGGGCAAG CCGCCGCGTG GAATTGGAAC GCGCCACCAT 
CTGCTACGCA ATCCGTCGCC AAGTTTCGGC ATACAGCAGC AGCGCATGGG CATGGCCGGG 
ATGCAACCGC CGCCACAGCG ATATGGCATG CCGGGCCAGT ACCCGGCAAC TCAGCCACCC 
AACCAGTCAC CACAACAAGC ACCGACCAAC ACACAACAGC CACACAGCGC TCCCGCCTCT 
CCCCTCCACA CTCCCACGCA TTCGCACATG ATGCACCACA GTCACAATCC GAACGGAGGC 
GTGCTGCACC ACCTGCTGCC GCATCCGCAC GAGCAGGTGC GTGTGGGTCT GCTGCAACCA 
CCGCCGCTGG CATATGCGCC CAATCCGGGT GGCTATCGCA ATGGAGCACT TCTGGGTCCG 
GGACCGGGAC CTGGTTTGCA TCAGCAACAC CAGTCGCCGC ACCAGCAACA GTCGGGCATG 
CGTCCGCCGC TGTATCGGAA TGCCCCGCCA TCGTACGGCT ACGACCAGGG ACATCCACCC 
CAGCATCCGC AGCAGTTCAT GCGGCCTCAC AACGGCTGGC GACCGCAGGG GGACAATGCG 
CGCATGCAGC GACCACCACT GCAAACGCTG CCACCGCACA TGATGCCCGG CGCACCGCCG 
AACTATGCCA ATGGCATGGG AATGCGAGGA CCGCCGCCCC AGCAACAGCA ATCGCAGCAG 
CCGCCACAGC AGCAGCCAGC ACCGCAGCAG GCGGGCAATC CCCTACAGCA GGCCGTCAAT 
CCTGGCCAGC CCCCTCAACA GCCGAATGGA CCGCCGCAGC AGCAGGGCTC ATCATCGGTG 
CCCCGGGTCA GCAAGGTGCT CATCAATCCG AATTTCAAGG GCGGCGTCGA AGCGGCTACC 
AACAAGTTCA TCAAGGAGAC ACACTTTATG CCCGCCATTA ACAGCGAGGC CGAGTTGCTG 
CGCCAGCAGG TGGAGTTCAT CAACAAGAAT CGGCAGTACA TTGAGAAGCG ACGCATGGAA 
CGCTCGCCAC CGTCGTCGGG CTCCTCCACC GGAGCCGCCT CGTCCGGCCA GGCAGGCGAA 
CGGAGGCGAA CACGTAGCCG CAGTCGCTCT CGTGGTCGCA GTTACTCTCC CGTTAAGCGC 
GTGGAAAGAG AACGCGAGCG GGAGCGGGAG CGCGAGAGGG ATCGTGAAAG GGATCGGGAG 
CGAGATCGTG AACGATATGG CGCCAATCGG GCGGCTGGAA ACCGTGGATT CCGACGGGCA 
TCGAGTCGGG ATCGAGACGA GAATCGTGGC GCAGCAAGCA CCGCCAGTGC TCAAGGAGCG 
GGCGGATCAG CATCCAGCGG AGCTGCGCCC CCCAAGCGAC GAAGGAGTGG CTCGGCCGGA 
GGAGGCACTG CCCGGAATCC ATTCTCTGGC GAGCCGCGCG GCCGACCCTC CGAAACAGGC 
AGTCGCAGTA TGCCCAGCGA CGAGGACGAG GAGACCCGCA ACTATCGCCT TGAGATTGAG 
AAGCAGAAGC AACTGCGCGA GAAAATCATG CGAGACAAGG AGCTGAAGCG TCGGCGCGCA 
GCCGAGGAGA AGCTACACGA GGAGAAACGC GCCGAGCAGC ACAACACTTC GCCAGCCCGC 
GACGACCAGG AGGTGGCCGC CGGACAAGGA TCTGCAGCGC CAACACCTGC AGCGTCAAAG 
CATCGGCCAA CCGCATCAGC CGGCGAGCGC AAGGTGATCT CGCTGAAGCG TCGCGACGAC 
CAGGATGCGA GCAGCGTACG CAGCAGGCAG GCACAGGGAT CGCAGTCCAA TGCTCAGGCA 
GGCCAAGGTC AGCAGCAGCA GCAGGCGGCG CGCAAGCAAC TAACGGCGGC GAAGATAGCG 
CCAGAGGCAA AGCGCAGTAT AACCGATCAC CTGGCGCCAT CCTCTAAGCA GCATCATCAT 
CATCATCAGC AGCATCATCA GACTGCAGCG GCGCCGGCGA GAACGGTGGT ATTGGGATCG 
GGAGCTGTGG CCGGCGGCAC ACCGCCGAAG CCGCGCGATA AGCTGCGCCT GGGCACGCCA 
AGCGACGATG AAGTGGAGCT GGACTATGAT GACGATGACC TGCTGCTGGA CGAGGAGGAT 
CGTTTGTTGG CCACGCCATC GCCGCCGCCG CAGAAGGCGA GCAGCAAAAG ATCGGCCACG 
CCGGAGCCCT TGGTGGTCAA GCGGAACCAC AAGGCGATGC GTGGCGGCAG CTCGACATCA 
GCGCCCAGCT TTAGCTCCAA CCAGCGGCGA GTGGTGCTCA AGCCGACCAG CAATGGCAGC 
AGCAGCAGCG GAGGAGATCA GCAGCAGTTG TCGCACAGCG GCAGGCAGCG GGATCGGAGG 
CGCGGTGGAG AGGATAGCGC ACTGCAGCGG AAAGGTGGAG TAGGAAGCGT TGGCGGCAGC 
AGCAGCTCGG GATCTGGCGG AGGGATCTTC GATAGGCTGG AGAAGCGTGT GGCCGTCTCA 
GGAGGAGGGA GCAGCGGTGG AGGCGGCGGG AGCAGCCACA AGCAGCGATC CAAGGCGCCC 
GCCCGCATCG TACTCAAGCA CGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG2186 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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