feo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	feo
Entrez Gene ID	32015
Full Name	fascetto
Synonyms	CG11207,Dmel\CG11207,EA86,Feo,PRC1,Q54,l(1)9Fe,l(1)BP1
General protein information	Preferred Names fascetto Names CG11207 gene product from transcript CG11207-RA CG11207-PA feo-PA lethal(1)9Fe
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9F5-9F5\|1-32.88 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_132430.3	NP_572658.2	fascetto

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	sofe	NP_572659.2
Drosophila melanogaster (fruit fly)	feo	NP_572658.2
Danio rerio (zebrafish)	prc1a	NP_001004494.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PRC1 binding to polycomb response elements is not strictly dependent on PRC2 and H3K27 methylation.
Title: Interdependence of PRC1 and PRC2 for recruitment to Polycomb Response Elements.

Feo may control organization, stability, and motor composition of antiparallel interpolar microtubules at midzone, thereby facilitating kinesin-5-driven sliding filament mechanism underlying proper anaphase B spindle elongation and chromosome segregation
Title: The microtubule cross-linker Feo controls the midzone stability, motor composition, and elongation of the anaphase B spindle in Drosophila embryos.

Data show that Sticky (Sti), tile ring component Citron kinase (CIT-K) interacts directly with two kinesins, Nebbish and Pavarotti and that in turn these kinesins interact with each other and with another central spindle protein, Fascetto.
Title: Citron kinase controls a molecular network required for midbody formation in cytokinesis.

Feo interacts with the plus ends of central spindle microtubules (MTs) so as to maintain their precise interdigitation during anaphase-telophase MT elongation and antiparallel sliding.
Title: Feo, the Drosophila homolog of PRC1, is required for central-spindle formation and cytokinesis.

The following feo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the feo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03438	NM_132430.3 Latest version!	Drosophila melanogaster fascetto (feo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

feo ( NM_132430.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572658.2 Drosophila melanogaster fascetto (feo), mRNA.

Price & Availability↑

CloneID	OFa03438
Clone ID Related Accession (Same CDS sequence)	NM_132430.3
Accession Version	NM_132430.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	fascetto
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132430.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCGC CGAGCGCCAT TGCGGTGAAG GCCCAGATCC TGGAGATGAC GGGCGAGCAC 
GTGGATCAGT TGCACGCGAT GTGGTCGCAG TTGTTCGAGG TCAAGACCTG CGGTGAGTTC 
CTCCTCCGGT TGAAGGACCA TGCGAACAGC TTCTTCACGG ATTTGCTCAA TGAGTCGCGC 
GAAAAGCAGC AGGCGATCCT CAACGATATA GCCGCCTTGC GGGCAGAGGC CACCGACTTG 
ACGCGCCTAC TCCACGAGCC GCTGGACATT GGCGAGCGGC CGGAGTCCAT GCCGCTGGTC 
GTCTGGGAGC TGAAGCTGGA CAAAAGCATC GAGCACTTAC GCGATCAGCT GGCCAGGCGG 
CGAGCGGAGA TCTGTGAGCT TCTGCTGCAG CAGGAGCAGC TGTGCGAGGA ACTGGGAGAG 
CTGCCTCTGC CACTGTTGGC GGATCCACTG CCGCTGCCCG AGGAGATGGA CGCCTTTCGG 
AATCGCCTGG GCCAGCTACG CGATCAGCGA GTGCTGCGCC TCAAAGAGAT GGACCAGCTG 
CGCCAGGCCA TTAAGCACGA CATGAAGCTG CTTGAGTGCC TGCCCCAGAC GGACACCGAG 
GAGCGTCTGC TCAACCAGGT GGATCACTGC CTAACGCCGG AGACCTTCGA GCTCCTGCGC 
AACATGCAAA AGAACTTCGC CGACCAGGTG AAGGAGCTGA GGGAGCGCAT CGACGATATG 
CGCGAAAAGA TCCATGTGCT GTGGGACAGA CTCCAGGAGA CAGACGAGTA TGCCATGCGT 
CGCGTGCGCG AGGCTACGAC CTACACTCAA CGCACCTACG ATGTCCTGCG GGAGGAACTG 
CAGCGCTGCC AGGCCCTGCG ACGCCAGAAT CTGAAAACCT TCATCGAGCA GCTACGCATC 
GAGATCAAGG AGATGTGGGA TCTCACACTT AAGAGCCAGC AGGAGCGCAA GCGCTTCTCG 
AACTACTACA ACGACTGGTA CAACGAAGAT CTGCTGGAGC TCCACGAACT TGAGCTGGAC 
GACCTCAAGA CCTTCTACAA CAAGAACAAG GAGATCTTCG ATCTGTACGA AAGCCGTGCC 
GAACTCTGGT CGCGCATGGA GGCGCTGGAG GCCAAGGCGA GCGAGCCGAA TCGCTTCAAC 
AATCGTGGCG GCCAGTTGCT CAAGGAGGAG AAGGAGCGCA AGGCCATCAC CTCCAAGCTG 
CCCAAGATTG AGCAGCAGAT CACGGAACTG GTGCAGGCAT ACGAGGCGCA GGAGAACACA 
CCCTTTCTGG TGCACGGCGA GAACATCCTG GAGCGGATGG CCGCCGATTG GGAGCGTCAT 
CGCCAGGCCA AACAGCAGAG CTCGGCGCGC AAGAAGGCAC CGCCATCGGC CAGCAAGATA 
ATGCCACCGC CGGCAACGGG CTCGACAGCT CCACGCACGC CGCGCACGCT GCGCAACATG 
TCGACCCTTT CCAGCTCCAC CATGTCGCTG CGCAAGACGC CGTCGCAGCA GCACCTCCGT 
CCACTAAACA TCACCAAGAG TACGGGCAAT CTGCACAAGC GCCTGAATCC GTCCGGCCTG 
CAGACGGGCC CCAAAACGCC CAACGCCAAG CGCAGCCTTA TGAACACACT CAACTCGATG 
AAGGCGTCGC CAGCCAAGCA CCTGCTCGCG CCCAGCCAGC AGCATAACCA GCTGAAGAGC 
ACCAAGTCGC CGCTGAAACG TGTTCGCGTA CTGGACAACA CACTGCGACG CAGCGGCGGT 
ATGGGCAGGC GCAGCATTGG AACGCGTTCC AGCAAGAAGG CGCGCACCAA GTCCAACGAT 
CAGAACGCCC CACCCGACAG CAGTGACTAC ACAACGGACG AGAATCCCGA GCAGGGTGTC 
AGCTATGACG AATTTCAGCC GACCAGTCGC TCCTCGATGC TGCCGCGCCA GCGTCATCAA 
TTGGCCGTGC CGCGAACTCG TCGTGCGGAA ATGAATGCCA ATCTGCCGGT GCCGCGGGAA 
AGCCTGATGA TCGTGCAGCC GCGCAGACAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572658.2
CDS	266..2281
Translation	MNSPSAIAVKAQILEMTGEHVDQLHAMWSQLFEVKTCGEFLLRLKDHANSFFTDLLNESREKQQAILNDIAALRAEATDLTRLLHEPLDIGERPESMPLVVWELKLDKSIEHLRDQLARRRAEICELLLQQEQLCEELGELPLPLLADPLPLPEEMDAFRNRLGQLRDQRVLRLKEMDQLRQAIKHDMKLLECLPQTDTEERLLNQVDHCLTPETFELLRNMQKNFADQVKELRERIDDMREKIHVLWDRLQETDEYAMRRVREATTYTQRTYDVLREELQRCQALRRQNLKTFIEQLRIEIKEMWDLTLKSQQERKRFSNYYNDWYNEDLLELHELELDDLKTFYNKNKEIFDLYESRAELWSRMEALEAKASEPNRFNNRGGQLLKEEKERKAITSKLPKIEQQITELVQAYEAQENTPFLVHGENILERMAADWERHRQAKQQSSARKKAPPSASKIMPPPATGSTAPRTPRTLRNMSTLSSSTMSLRKTPSQQHLRPLNITKSTGNLHKRLNPSGLQTGPKTPNAKRSLMNTLNSMKASPAKHLLAPSQQHNQLKSTKSPLKRVRVLDNTLRRSGGMGRRSIGTRSSKKARTKSNDQNAPPDSSDYTTDENPEQGVSYDEFQPTSRSSMLPRQRHQLAVPRTRRAEMNANLPVPRESLMIVQPRRQF

Target ORF information:

RefSeq Version	NM_132430.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster fascetto (feo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132430.3

ORF Insert Sequence:

ATGAACTCGC CGAGCGCCAT TGCGGTGAAG GCCCAGATCC TGGAGATGAC GGGCGAGCAC 
GTGGATCAGT TGCACGCGAT GTGGTCGCAG TTGTTCGAGG TCAAGACCTG CGGTGAGTTC 
CTCCTCCGGT TGAAGGACCA TGCGAACAGC TTCTTCACGG ATTTGCTCAA TGAGTCGCGC 
GAAAAGCAGC AGGCGATCCT CAACGATATA GCCGCCTTGC GGGCAGAGGC CACCGACTTG 
ACGCGCCTAC TCCACGAGCC GCTGGACATT GGCGAGCGGC CGGAGTCCAT GCCGCTGGTC 
GTCTGGGAGC TGAAGCTGGA CAAAAGCATC GAGCACTTAC GCGATCAGCT GGCCAGGCGG 
CGAGCGGAGA TCTGTGAGCT TCTGCTGCAG CAGGAGCAGC TGTGCGAGGA ACTGGGAGAG 
CTGCCTCTGC CACTGTTGGC GGATCCACTG CCGCTGCCCG AGGAGATGGA CGCCTTTCGG 
AATCGCCTGG GCCAGCTACG CGATCAGCGA GTGCTGCGCC TCAAAGAGAT GGACCAGCTG 
CGCCAGGCCA TTAAGCACGA CATGAAGCTG CTTGAGTGCC TGCCCCAGAC GGACACCGAG 
GAGCGTCTGC TCAACCAGGT GGATCACTGC CTAACGCCGG AGACCTTCGA GCTCCTGCGC 
AACATGCAAA AGAACTTCGC CGACCAGGTG AAGGAGCTGA GGGAGCGCAT CGACGATATG 
CGCGAAAAGA TCCATGTGCT GTGGGACAGA CTCCAGGAGA CAGACGAGTA TGCCATGCGT 
CGCGTGCGCG AGGCTACGAC CTACACTCAA CGCACCTACG ATGTCCTGCG GGAGGAACTG 
CAGCGCTGCC AGGCCCTGCG ACGCCAGAAT CTGAAAACCT TCATCGAGCA GCTACGCATC 
GAGATCAAGG AGATGTGGGA TCTCACACTT AAGAGCCAGC AGGAGCGCAA GCGCTTCTCG 
AACTACTACA ACGACTGGTA CAACGAAGAT CTGCTGGAGC TCCACGAACT TGAGCTGGAC 
GACCTCAAGA CCTTCTACAA CAAGAACAAG GAGATCTTCG ATCTGTACGA AAGCCGTGCC 
GAACTCTGGT CGCGCATGGA GGCGCTGGAG GCCAAGGCGA GCGAGCCGAA TCGCTTCAAC 
AATCGTGGCG GCCAGTTGCT CAAGGAGGAG AAGGAGCGCA AGGCCATCAC CTCCAAGCTG 
CCCAAGATTG AGCAGCAGAT CACGGAACTG GTGCAGGCAT ACGAGGCGCA GGAGAACACA 
CCCTTTCTGG TGCACGGCGA GAACATCCTG GAGCGGATGG CCGCCGATTG GGAGCGTCAT 
CGCCAGGCCA AACAGCAGAG CTCGGCGCGC AAGAAGGCAC CGCCATCGGC CAGCAAGATA 
ATGCCACCGC CGGCAACGGG CTCGACAGCT CCACGCACGC CGCGCACGCT GCGCAACATG 
TCGACCCTTT CCAGCTCCAC CATGTCGCTG CGCAAGACGC CGTCGCAGCA GCACCTCCGT 
CCACTAAACA TCACCAAGAG TACGGGCAAT CTGCACAAGC GCCTGAATCC GTCCGGCCTG 
CAGACGGGCC CCAAAACGCC CAACGCCAAG CGCAGCCTTA TGAACACACT CAACTCGATG 
AAGGCGTCGC CAGCCAAGCA CCTGCTCGCG CCCAGCCAGC AGCATAACCA GCTGAAGAGC 
ACCAAGTCGC CGCTGAAACG TGTTCGCGTA CTGGACAACA CACTGCGACG CAGCGGCGGT 
ATGGGCAGGC GCAGCATTGG AACGCGTTCC AGCAAGAAGG CGCGCACCAA GTCCAACGAT 
CAGAACGCCC CACCCGACAG CAGTGACTAC ACAACGGACG AGAATCCCGA GCAGGGTGTC 
AGCTATGACG AATTTCAGCC GACCAGTCGC TCCTCGATGC TGCCGCGCCA GCGTCATCAA 
TTGGCCGTGC CGCGAACTCG TCGTGCGGAA ATGAATGCCA ATCTGCCGGT GCCGCGGGAA 
AGCCTGATGA TCGTGCAGCC GCGCAGACAG TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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feo cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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