btd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	btd
Entrez Gene ID	31912
Full Name	buttonhead
Synonyms	BTD,Btd,CG12653,Dmel\CG12653
General protein information	Preferred Names buttonhead Names CG12653 gene product from transcript CG12653-RA CG12653-PA btd-PA button-head
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8F10-8F10\|1-31 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_078545.3	NP_511100.1	buttonhead

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Btd prevents the premature differentiation by suppressing the expression of Prospero in immature intermediate neural progenitor cells. Btd functions cooperatively with Pointed P1 to promote the generation of the above cells.
Title: The Drosophila Sp8 transcription factor Buttonhead prevents premature differentiation of intermediate neural progenitors.

Trithorax maintains the functional heterogeneity of neural stem cells through the transcription factor buttonhead.
Title: Trithorax maintains the functional heterogeneity of neural stem cells through the transcription factor buttonhead.

By simultaneously removing the function of two Sp8 orthologs, buttonhead (btd) and Sp1, during Drosophila embryogenesis, we find that adult leg development is completely abolished.
Title: Non-redundant selector and growth-promoting functions of two sister genes, buttonhead and Sp1, in Drosophila leg development.

Results show that buttonhead and Sp1 induce the formation and are required for the growth of the ventral imaginal discs.
Title: The role of buttonhead and Sp1 in the development of the ventral imaginal discs of Drosophila.

The following btd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the btd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03367	NM_078545.3 Latest version!	Drosophila melanogaster buttonhead (btd), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

btd ( NM_078545.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511100.1 Drosophila melanogaster buttonhead (btd), mRNA.

Price & Availability↑

CloneID	OFa03367
Clone ID Related Accession (Same CDS sequence)	NM_078545.3
Accession Version	NM_078545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	buttonhead
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078545.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCGATG CGGCCTGCAA TTACTTAAAT CCCTATGCGC AACAGCATCA GGCGCAGCAG 
CAGCAACATG CGCAACATCA GCAACACGCG CAACAACAGC AACATCATCT GCACATGCAG 
CAGGCGCAAC ATCATCTGCA CTTGTCGCAT CAGCAGGCGC AACAGCAGCA TATGCAACAC 
TTGACACAGC AACAGCAGCA GCAGCAACAA CAACAGCAGC AACAGCAGCA GCAACAGCAG 
CAACAACAAC AGCCGCAACA GCAGCAACAT GACTTCCTCA GCGCCGCCGC CCTGCTGTCC 
GCCCCACCAT CCCTATCCGG CTCGAGTTCC GGCTCCAGCT CCGGCAGTTC TCCGCTATAT 
GGAAAGCCGC CAATGAAGCT GGAACTGCCG TATCCACAGG CCTCATCCAC GGGCACCGCA 
TCGCCCAACT CCAGCATACA ATCGGCGCCA TCATCGGCCT CCGTGTCGCC CAGCATCTTC 
CCATCACCTG CCCAGTCATT CGCCTCCATT TCGGCTAGTC CATCCACGCC CACCACGACC 
TTGGCGCCAC CAACAACGGC GGCAGCGGGC GCCCTAGCGG GATCACCCAC ATCCTCCTCA 
CCATCCTCGT CAGCAGCATC GGCTGCAGCG GCGGCAGCAG CAGCTGCGGC GGCAGCAGCG 
GACCTTGGAG CAGCGGCGGT CGCCAGTGCC GCCTACGGCT GGAATACCGC CTACTCCGGA 
TTGGGGCCAG CGAGAAGTCA GTTTCCATAT GCCCAGTACG CCTCCGATTA TTACGGCAAT 
GCGGTGGGCA TGTCCTCCTC GGCCGCTTGG TTCTCGCATC AGGAGCGCCT CTATCAGCCA 
TGGAGTTCAC AGAGCTATCC GGGCTTCAAT TTCGACGACA TCGCCTTCCA GACGCAATTG 
CAACGCCGCT CCGTTCGCTG CACGTGTCCC AATTGCACCA ACGAGATGAG CGGCCTGCCA 
CCGATTGTGG GTCCGGATGA GCGCGGACGC AAGCAGCATA TCTGCCACAT TCCGGGCTGC 
GAACGGCTAT ACGGTAAGGC GTCGCATCTG AAGACCCACC TGCGCTGGCA CACCGGCGAG 
CGGCCCTTCC TGTGCCTCAC GTGCGGCAAG CGTTTCTCCC GATCGGATGA GCTGCAGCGG 
CACGGACGTA CGCACACCAA TTACCGCCCG TATGCCTGCC CCATTTGCTC CAAGAAGTTT 
TCGCGCAGCG ATCACCTCAG CAAGCACAAG AAGACCCATT TCAAGGACAA GAAGAGCAAG 
AAGGTATTGG CCGCCGAGGC CAAGGAGCAG GCGGCTGCGG CGATAAAGCT GGAAAAGAAG 
GAGAAGAAGT CGGGTAAGCC ACTGACGCCA CCCGTGGAAT TCAAGCAGGA GCAGCCGGAT 
ACGACACCGC TGGTCAACTA TGCGCCCTAT GCCAATCTCT ATCAGCATTC CACATCCGCC 
GGATCGAGTG TGAATCCTCC GCCGCCACCG CCGCCGCTCT TCCAGCAGCA GATGACGACG 
ACAACATCAT CGGCGGCGGC CAGCTTTGTG GAGCAGCCGT GGAGCAGTAG CAGCAGCCGT 
GCCATACAAC CAGCCACTAC CTCAGCCAGT TCATCCTCAT CCTCCAGCGC CAGTTCCCCA 
GCGGCAGCGG TCGTTTCTGC CATTGGATCT GCATCTTCCC CAGCCGCATC AGCCACGGCG 
CTGGCCCAGC ATCATTATGC CGCACTTGCC ATGCAAAGTG AGTCCCAGCT GGCGGCGGAA 
TATGGGCTAA CCATGAGCGG ATTGGCCAGC GGAGCTAGCC AGGATTCCAG CTCCAGTTGC 
CACATGAAGT CCGAGTATGC GGCCAGTTAT CCGGCTGATT TTGGTGCGGG AACAGCCAGT 
TACGGTTATC CGCATCCACA TCCGCACCAT CACAATGCGT GGGCAGCGGC CTATCATCCA 
CATGCCACCG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511100.1
CDS	489..2423
Translation	MIDAACNYLNPYAQQHQAQQQQHAQHQQHAQQQQHHLHMQQAQHHLHLSHQQAQQQHMQHLTQQQQQQQQQQQQQQQQQQQQQQPQQQQHDFLSAAALLSAPPSLSGSSSGSSSGSSPLYGKPPMKLELPYPQASSTGTASPNSSIQSAPSSASVSPSIFPSPAQSFASISASPSTPTTTLAPPTTAAAGALAGSPTSSSPSSSAASAAAAAAAAAAAAADLGAAAVASAAYGWNTAYSGLGPARSQFPYAQYASDYYGNAVGMSSSAAWFSHQERLYQPWSSQSYPGFNFDDIAFQTQLQRRSVRCTCPNCTNEMSGLPPIVGPDERGRKQHICHIPGCERLYGKASHLKTHLRWHTGERPFLCLTCGKRFSRSDELQRHGRTHTNYRPYACPICSKKFSRSDHLSKHKKTHFKDKKSKKVLAAEAKEQAAAAIKLEKKEKKSGKPLTPPVEFKQEQPDTTPLVNYAPYANLYQHSTSAGSSVNPPPPPPPLFQQQMTTTTSSAAASFVEQPWSSSSSRAIQPATTSASSSSSSSASSPAAAVVSAIGSASSPAASATALAQHHYAALAMQSESQLAAEYGLTMSGLASGASQDSSSSCHMKSEYAASYPADFGAGTASYGYPHPHPHHHNAWAAAYHPHATA

Target ORF information:

RefSeq Version	NM_078545.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster buttonhead (btd), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078545.3

ORF Insert Sequence:

ATGATCGATG CGGCCTGCAA TTACTTAAAT CCCTATGCGC AACAGCATCA GGCGCAGCAG 
CAGCAACATG CGCAACATCA GCAACACGCG CAACAACAGC AACATCATCT GCACATGCAG 
CAGGCGCAAC ATCATCTGCA CTTGTCGCAT CAGCAGGCGC AACAGCAGCA TATGCAACAC 
TTGACACAGC AACAGCAGCA GCAGCAACAA CAACAGCAGC AACAGCAGCA GCAACAGCAG 
CAACAACAAC AGCCGCAACA GCAGCAACAT GACTTCCTCA GCGCCGCCGC CCTGCTGTCC 
GCCCCACCAT CCCTATCCGG CTCGAGTTCC GGCTCCAGCT CCGGCAGTTC TCCGCTATAT 
GGAAAGCCGC CAATGAAGCT GGAACTGCCG TATCCACAGG CCTCATCCAC GGGCACCGCA 
TCGCCCAACT CCAGCATACA ATCGGCGCCA TCATCGGCCT CCGTGTCGCC CAGCATCTTC 
CCATCACCTG CCCAGTCATT CGCCTCCATT TCGGCTAGTC CATCCACGCC CACCACGACC 
TTGGCGCCAC CAACAACGGC GGCAGCGGGC GCCCTAGCGG GATCACCCAC ATCCTCCTCA 
CCATCCTCGT CAGCAGCATC GGCTGCAGCG GCGGCAGCAG CAGCTGCGGC GGCAGCAGCG 
GACCTTGGAG CAGCGGCGGT CGCCAGTGCC GCCTACGGCT GGAATACCGC CTACTCCGGA 
TTGGGGCCAG CGAGAAGTCA GTTTCCATAT GCCCAGTACG CCTCCGATTA TTACGGCAAT 
GCGGTGGGCA TGTCCTCCTC GGCCGCTTGG TTCTCGCATC AGGAGCGCCT CTATCAGCCA 
TGGAGTTCAC AGAGCTATCC GGGCTTCAAT TTCGACGACA TCGCCTTCCA GACGCAATTG 
CAACGCCGCT CCGTTCGCTG CACGTGTCCC AATTGCACCA ACGAGATGAG CGGCCTGCCA 
CCGATTGTGG GTCCGGATGA GCGCGGACGC AAGCAGCATA TCTGCCACAT TCCGGGCTGC 
GAACGGCTAT ACGGTAAGGC GTCGCATCTG AAGACCCACC TGCGCTGGCA CACCGGCGAG 
CGGCCCTTCC TGTGCCTCAC GTGCGGCAAG CGTTTCTCCC GATCGGATGA GCTGCAGCGG 
CACGGACGTA CGCACACCAA TTACCGCCCG TATGCCTGCC CCATTTGCTC CAAGAAGTTT 
TCGCGCAGCG ATCACCTCAG CAAGCACAAG AAGACCCATT TCAAGGACAA GAAGAGCAAG 
AAGGTATTGG CCGCCGAGGC CAAGGAGCAG GCGGCTGCGG CGATAAAGCT GGAAAAGAAG 
GAGAAGAAGT CGGGTAAGCC ACTGACGCCA CCCGTGGAAT TCAAGCAGGA GCAGCCGGAT 
ACGACACCGC TGGTCAACTA TGCGCCCTAT GCCAATCTCT ATCAGCATTC CACATCCGCC 
GGATCGAGTG TGAATCCTCC GCCGCCACCG CCGCCGCTCT TCCAGCAGCA GATGACGACG 
ACAACATCAT CGGCGGCGGC CAGCTTTGTG GAGCAGCCGT GGAGCAGTAG CAGCAGCCGT 
GCCATACAAC CAGCCACTAC CTCAGCCAGT TCATCCTCAT CCTCCAGCGC CAGTTCCCCA 
GCGGCAGCGG TCGTTTCTGC CATTGGATCT GCATCTTCCC CAGCCGCATC AGCCACGGCG 
CTGGCCCAGC ATCATTATGC CGCACTTGCC ATGCAAAGTG AGTCCCAGCT GGCGGCGGAA 
TATGGGCTAA CCATGAGCGG ATTGGCCAGC GGAGCTAGCC AGGATTCCAG CTCCAGTTGC 
CACATGAAGT CCGAGTATGC GGCCAGTTAT CCGGCTGATT TTGGTGCGGG AACAGCCAGT 
TACGGTTATC CGCATCCACA TCCGCACCAT CACAATGCGT GGGCAGCGGC CTATCATCCA 
CATGCCACCG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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btd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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