lz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	lz
Entrez Gene ID	31883
Full Name	lozenge
Synonyms	CG1689,Dmel\CG1689,Lz,amx,fs(1)A1569,fs(1)M69,spe
General protein information	Preferred Names lozenge Names CG1689 gene product from transcript CG1689-RA CG1689-PA CG1689-PB lz-PA lz-PB spectacled
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8D5-8D6\|1-27.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103449.2	NP_001096919.1	lozenge, isoform B
NM_078544.3	NP_511099.2	lozenge, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our data indicate that the MLF/DnaJ-1-dependent increase in Lz level allows the repression of Notch expression and signaling to prevent aberrant blood cell development. Thus our findings establish a functional link between MLF and the co-chaperone DnaJ-1 to control RUNX transcription factor activity and Notch signaling during blood cell development in vivo.
Title: Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.

Identification of the Doublesex protein binding sites that activate expression of lozenge in the female genital disc in Drosophila melanogaster has been reported.
Title: Identification of the Doublesex protein binding sites that activate expression of lozenge in the female genital disc in Drosophila melanogaster.

Lz provides key intrinsic information to cells during the second wave of pattern formation in the developing ommatidium.
Title: Switching cell fates in the developing Drosophila eye.

Notch cooperates with Lozenge/Runx to lock haemocytes into a differentiation programme.
Title: Notch cooperates with Lozenge/Runx to lock haemocytes into a differentiation programme.

mlf participates in a positive feedback loop to fine tune the activity of the RUNX transcription factor Lozenge (LZ) during development of the crystal cells.
Title: Myeloid leukemia factor is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis.

The following lz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03346	NM_078544.3 Latest version!	Drosophila melanogaster lozenge (lz), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa03347	NM_001103449.2 Latest version!	Drosophila melanogaster lozenge (lz), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lz ( NM_078544.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511099.2 Drosophila melanogaster lozenge (lz), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03346
Clone ID Related Accession (Same CDS sequence)	NM_078544.3
Accession Version	NM_078544.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lozenge, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078544.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTGC ATCTCCTGGC GGCAGAGAAG ACGCCTCCAT CTCCATCTCC AAATCCAACT 
CCAACTCCAT CAGCATCTCC ATCTGGGCAT ACGGGCGCTG CTGGGGAGTC GCAATTGGAC 
TTGGCATCGC AAACGGAATC GCAATTGCAG CTGGGCTTGC CATTGGCTTC CGGATACGGA 
CTCGGACTCG GATTGGGACT GGGCTTGGGA TTGGGACTGG GCCAGGAATT GGTGGCGGAC 
CACAGCACCA CTGTGGCGCC CGTCAGCGTT GCCGGTCCCG GCCGCCTGGG TCGCAGCATC 
AACGGCAGCG GCGGCAGCCA CCACCATCAC CACCTGCACC ACCACTACTC ACCGTACCAC 
CACGCCCACC CCTACCACCC ACCGCATCCG CACGCGCCCC ACCACCACCA CCACCACCAC 
CCACCGTATC CGTATCCGCC GGCAGGACCA CATCCGCCGG CCATGGTCAC ATCGTCATCC 
ACATCGCCGA CGGGCAACGG CTGGAGCAGC TCCACCGGCG ACTTCAAGGG CATCACAGCG 
GTGGCGACGG CCACTGGCGG CGGCGTAGGT GGTGCCACCC AGGGCGCCAC TGCATCCACG 
GGCGCCACAG CCGCTGAGGT TTTGGCCGTT TCGAGTAGTG CCAGTGTTGG CAGCAGCTCG 
CCCACAGGTG GCGCCAGCAA TGGTACAGCC CATAGTGGGC ATAGTGGACA CACCGGCGGT 
CACAGCAGTA GTACCGCCAG CAACAACAAC AACAACGGTG CCAGCAATAG CAATAGCAAC 
AATAACAACG CCGTTCACCA GGATCTATTG TGGATGGAGC GATTGGTACA GAAGCGACAA 
CAGGAACATC CCGGCGAATT GGTGCGCACG AGCAATCCGT ACTTCCTGTG CTCCGCCCTG 
CCCGCCCATT GGCGCTCCAA CAAGACGCTG CCGATGGCCT TCAAGGTCGT CGCTCTGGCG 
GAAGTGGGCG ATGGCACCTA TGTCACCATC CGGGCGGGAA ACGATGAGAA TTGCTGCGCG 
GAGCTGCGCA ACTTCACCAC CCAGATGAAG AACGACGTGG CCAAGTTCAA TGATCTGCGT 
TTCGTGGGAC GCAGCGGAAG AGGGAAGAGC TTCACGCTGA CCATCACGGT GGCCACGAGT 
CCGCCACAGG TGGCAACCTA CGCCAAAGCC ATCAAGGTGA CCGTCGATGG ACCCCGCGAG 
CCCCGCTCCA AGACAAGTCC GACCGGTGGT CCGCACTACC GAGCTCTGGG TCTGGGCCAG 
CGTCCCTACA TCGATGGCTT CCCCTCGACG AAGGCGCTCC ACGAGCTGGA GTCGCTCCGG 
CGGTCCGCCA AGGTGGCAGC GGTGACGACG GCAGCGGCGG CGGCGGCCAC AGCGGCATCC 
GCGGCCAATG CGGTGGCGGC GGCCGCAGCC GCTGTGGCGG TAACTCCAAC GGGGGGCGGC 
GGCGGCGTTG CAGCTGGGGG CGTGGCTGGC GGAGCAGGCG CTGGACTCGT GCAGCAATTG 
AGCAGCAATT ACTCATCGCC GAATAGTACA ATCAACTCGG ACTGCCAGGT CTACAAGCCG 
AATGCGCCGC ACATACAAGC AGCCGAAATG ATGGGCGCCG GCGAATGGAC AAATGGATCC 
TCGAGTTCGG CGGCTGCCTA CTACCACAGC CACGCCCATC ATCCGCACGC CCACCACGCC 
CACGCGCACC TGCAACACCA GATGGCCTTG CCACCGCCGC CGCCGCCACC CGCTGCGGCA 
CCCGTTTCGG TGGGCGTGGG CGGCAATGGA GCCACCATGG GCATGGGCAT GGGAGTGGGA 
GTGGGCATGG GCATGAATCA CTACGGCGGT GGCTACGACT CGGCCAACTC GCTGGAGGCG 
GGCCAGTATG CCGCCCACCT GCCGGCGGTG CTGCCCGAGA TGCATGGCCA TGGATTCGCC 
ACCGATCCCT ACCAGACGGC CGGCTATGGC GGTGGCAATA CGGGCGGAGG CAGTGCATCC 
AAATCGGAAC TGGACTATGG CGGCAGCTAC AATCAGGCGT GGTCGAATGG CTATCAGAAC 
TATCAGTATG GCAGTTGTTT GGCCACCGCC CAGTACGGTC CTCAGGCGGC GCCGCCACCA 
CAGCCACCGC CTCCGCCGCC GGTGGTGCTG TGCCCGCAGC TGTACTCCAC GGTCAATCAG 
AATCAAATCC ACCTGCATCT GCACAGCAGC GAGAAGCTGG AGCAGTATCT GGGCACGGCC 
ACTAGTGCGG ACCATCTCAC CATTGGATCC CTGACGGGCA GCAGTCGTTC CAGCATTGAA 
ATTGGCCAGG ATCAGTACCA CCAGCAGGTG CACCACGCAC AGCAGCAGCA ACAGCAGCAG 
CAGCAACAAC AACAGGTGCA CCACCCACAG CAGCAGCAGG TGGAATCCGC TGGCGAAGTG 
GGTGGCAGTG GTGCCGGCGG AGTGGAATCC GCCAGGGAGG AGGACGTGGG CGATCTCTCG 
CAGGTGTGGC GACCCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511099.2
CDS	223..2703
Translation	MHLHLLAAEKTPPSPSPNPTPTPSASPSGHTGAAGESQLDLASQTESQLQLGLPLASGYGLGLGLGLGLGLGLGQELVADHSTTVAPVSVAGPGRLGRSINGSGGSHHHHHLHHHYSPYHHAHPYHPPHPHAPHHHHHHHPPYPYPPAGPHPPAMVTSSSTSPTGNGWSSSTGDFKGITAVATATGGGVGGATQGATASTGATAAEVLAVSSSASVGSSSPTGGASNGTAHSGHSGHTGGHSSSTASNNNNNGASNSNSNNNNAVHQDLLWMERLVQKRQQEHPGELVRTSNPYFLCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTITVATSPPQVATYAKAIKVTVDGPREPRSKTSPTGGPHYRALGLGQRPYIDGFPSTKALHELESLRRSAKVAAVTTAAAAAATAASAANAVAAAAAAVAVTPTGGGGGVAAGGVAGGAGAGLVQQLSSNYSSPNSTINSDCQVYKPNAPHIQAAEMMGAGEWTNGSSSSAAAYYHSHAHHPHAHHAHAHLQHQMALPPPPPPPAAAPVSVGVGGNGATMGMGMGVGVGMGMNHYGGGYDSANSLEAGQYAAHLPAVLPEMHGHGFATDPYQTAGYGGGNTGGGSASKSELDYGGSYNQAWSNGYQNYQYGSCLATAQYGPQAAPPPQPPPPPPVVLCPQLYSTVNQNQIHLHLHSSEKLEQYLGTATSADHLTIGSLTGSSRSSIEIGQDQYHQQVHHAQQQQQQQQQQQQVHHPQQQQVESAGEVGGSGAGGVESAREEDVGDLSQVWRPY

Target ORF information:

RefSeq Version	NM_078544.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lozenge (lz), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078544.3

ORF Insert Sequence:

ATGCATTTGC ATCTCCTGGC GGCAGAGAAG ACGCCTCCAT CTCCATCTCC AAATCCAACT 
CCAACTCCAT CAGCATCTCC ATCTGGGCAT ACGGGCGCTG CTGGGGAGTC GCAATTGGAC 
TTGGCATCGC AAACGGAATC GCAATTGCAG CTGGGCTTGC CATTGGCTTC CGGATACGGA 
CTCGGACTCG GATTGGGACT GGGCTTGGGA TTGGGACTGG GCCAGGAATT GGTGGCGGAC 
CACAGCACCA CTGTGGCGCC CGTCAGCGTT GCCGGTCCCG GCCGCCTGGG TCGCAGCATC 
AACGGCAGCG GCGGCAGCCA CCACCATCAC CACCTGCACC ACCACTACTC ACCGTACCAC 
CACGCCCACC CCTACCACCC ACCGCATCCG CACGCGCCCC ACCACCACCA CCACCACCAC 
CCACCGTATC CGTATCCGCC GGCAGGACCA CATCCGCCGG CCATGGTCAC ATCGTCATCC 
ACATCGCCGA CGGGCAACGG CTGGAGCAGC TCCACCGGCG ACTTCAAGGG CATCACAGCG 
GTGGCGACGG CCACTGGCGG CGGCGTAGGT GGTGCCACCC AGGGCGCCAC TGCATCCACG 
GGCGCCACAG CCGCTGAGGT TTTGGCCGTT TCGAGTAGTG CCAGTGTTGG CAGCAGCTCG 
CCCACAGGTG GCGCCAGCAA TGGTACAGCC CATAGTGGGC ATAGTGGACA CACCGGCGGT 
CACAGCAGTA GTACCGCCAG CAACAACAAC AACAACGGTG CCAGCAATAG CAATAGCAAC 
AATAACAACG CCGTTCACCA GGATCTATTG TGGATGGAGC GATTGGTACA GAAGCGACAA 
CAGGAACATC CCGGCGAATT GGTGCGCACG AGCAATCCGT ACTTCCTGTG CTCCGCCCTG 
CCCGCCCATT GGCGCTCCAA CAAGACGCTG CCGATGGCCT TCAAGGTCGT CGCTCTGGCG 
GAAGTGGGCG ATGGCACCTA TGTCACCATC CGGGCGGGAA ACGATGAGAA TTGCTGCGCG 
GAGCTGCGCA ACTTCACCAC CCAGATGAAG AACGACGTGG CCAAGTTCAA TGATCTGCGT 
TTCGTGGGAC GCAGCGGAAG AGGGAAGAGC TTCACGCTGA CCATCACGGT GGCCACGAGT 
CCGCCACAGG TGGCAACCTA CGCCAAAGCC ATCAAGGTGA CCGTCGATGG ACCCCGCGAG 
CCCCGCTCCA AGACAAGTCC GACCGGTGGT CCGCACTACC GAGCTCTGGG TCTGGGCCAG 
CGTCCCTACA TCGATGGCTT CCCCTCGACG AAGGCGCTCC ACGAGCTGGA GTCGCTCCGG 
CGGTCCGCCA AGGTGGCAGC GGTGACGACG GCAGCGGCGG CGGCGGCCAC AGCGGCATCC 
GCGGCCAATG CGGTGGCGGC GGCCGCAGCC GCTGTGGCGG TAACTCCAAC GGGGGGCGGC 
GGCGGCGTTG CAGCTGGGGG CGTGGCTGGC GGAGCAGGCG CTGGACTCGT GCAGCAATTG 
AGCAGCAATT ACTCATCGCC GAATAGTACA ATCAACTCGG ACTGCCAGGT CTACAAGCCG 
AATGCGCCGC ACATACAAGC AGCCGAAATG ATGGGCGCCG GCGAATGGAC AAATGGATCC 
TCGAGTTCGG CGGCTGCCTA CTACCACAGC CACGCCCATC ATCCGCACGC CCACCACGCC 
CACGCGCACC TGCAACACCA GATGGCCTTG CCACCGCCGC CGCCGCCACC CGCTGCGGCA 
CCCGTTTCGG TGGGCGTGGG CGGCAATGGA GCCACCATGG GCATGGGCAT GGGAGTGGGA 
GTGGGCATGG GCATGAATCA CTACGGCGGT GGCTACGACT CGGCCAACTC GCTGGAGGCG 
GGCCAGTATG CCGCCCACCT GCCGGCGGTG CTGCCCGAGA TGCATGGCCA TGGATTCGCC 
ACCGATCCCT ACCAGACGGC CGGCTATGGC GGTGGCAATA CGGGCGGAGG CAGTGCATCC 
AAATCGGAAC TGGACTATGG CGGCAGCTAC AATCAGGCGT GGTCGAATGG CTATCAGAAC 
TATCAGTATG GCAGTTGTTT GGCCACCGCC CAGTACGGTC CTCAGGCGGC GCCGCCACCA 
CAGCCACCGC CTCCGCCGCC GGTGGTGCTG TGCCCGCAGC TGTACTCCAC GGTCAATCAG 
AATCAAATCC ACCTGCATCT GCACAGCAGC GAGAAGCTGG AGCAGTATCT GGGCACGGCC 
ACTAGTGCGG ACCATCTCAC CATTGGATCC CTGACGGGCA GCAGTCGTTC CAGCATTGAA 
ATTGGCCAGG ATCAGTACCA CCAGCAGGTG CACCACGCAC AGCAGCAGCA ACAGCAGCAG 
CAGCAACAAC AACAGGTGCA CCACCCACAG CAGCAGCAGG TGGAATCCGC TGGCGAAGTG 
GGTGGCAGTG GTGCCGGCGG AGTGGAATCC GCCAGGGAGG AGGACGTGGG CGATCTCTCG 
CAGGTGTGGC GACCCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lz ( NM_001103449.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096919.1 Drosophila melanogaster lozenge (lz), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03347
Clone ID Related Accession (Same CDS sequence)	NM_001103449.2
Accession Version	NM_001103449.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lozenge, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103449.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTGC ATCTCCTGGC GGCAGAGAAG ACGCCTCCAT CTCCATCTCC AAATCCAACT 
CCAACTCCAT CAGCATCTCC ATCTGGGCAT ACGGGCGCTG CTGGGGAGTC GCAATTGGAC 
TTGGCATCGC AAACGGAATC GCAATTGCAG CTGGGCTTGC CATTGGCTTC CGGATACGGA 
CTCGGACTCG GATTGGGACT GGGCTTGGGA TTGGGACTGG GCCAGGAATT GGTGGCGGAC 
CACAGCACCA CTGTGGCGCC CGTCAGCGTT GCCGGTCCCG GCCGCCTGGG TCGCAGCATC 
AACGGCAGCG GCGGCAGCCA CCACCATCAC CACCTGCACC ACCACTACTC ACCGTACCAC 
CACGCCCACC CCTACCACCC ACCGCATCCG CACGCGCCCC ACCACCACCA CCACCACCAC 
CCACCGTATC CGTATCCGCC GGCAGGACCA CATCCGCCGG CCATGGTCAC ATCGTCATCC 
ACATCGCCGA CGGGCAACGG CTGGAGCAGC TCCACCGGCG ACTTCAAGGG CATCACAGCG 
GTGGCGACGG CCACTGGCGG CGGCGTAGGT GGTGCCACCC AGGGCGCCAC TGCATCCACG 
GGCGCCACAG CCGCTGAGGT TTTGGCCGTT TCGAGTAGTG CCAGTGTTGG CAGCAGCTCG 
CCCACAGGTG GCGCCAGCAA TGGTACAGCC CATAGTGGGC ATAGTGGACA CACCGGCGGT 
CACAGCAGTA GTACCGCCAG CAACAACAAC AACAACGGTG CCAGCAATAG CAATAGCAAC 
AATAACAACG CCGTTCACCA GGATCTATTG TGGATGGAGC GATTGGTACA GAAGCGACAA 
CAGGAACATC CCGGCGAATT GGTGCGCACG AGCAATCCGT ACTTCCTGTG CTCCGCCCTG 
CCCGCCCATT GGCGCTCCAA CAAGACGCTG CCGATGGCCT TCAAGGTCGT CGCTCTGGCG 
GAAGTGGGCG ATGGCACCTA TGTCACCATC CGGGCGGGAA ACGATGAGAA TTGCTGCGCG 
GAGCTGCGCA ACTTCACCAC CCAGATGAAG AACGACGTGG CCAAGTTCAA TGATCTGCGT 
TTCGTGGGAC GCAGCGGAAG AGGGAAGAGC TTCACGCTGA CCATCACGGT GGCCACGAGT 
CCGCCACAGG TGGCAACCTA CGCCAAAGCC ATCAAGGTGA CCGTCGATGG ACCCCGCGAG 
CCCCGCTCCA AGACAACAGC CGAAATGATG GGCGCCGGCG AATGGACAAA TGGATCCTCG 
AGTTCGGCGG CTGCCTACTA CCACAGCCAC GCCCATCATC CGCACGCCCA CCACGCCCAC 
GCGCACCTGC AACACCAGAT GGCCTTGCCA CCGCCGCCGC CGCCACCCGC TGCGGCACCC 
GTTTCGGTGG GCGTGGGCGG CAATGGAGCC ACCATGGGCA TGGGCATGGG AGTGGGAGTG 
GGCATGGGCA TGAATCACTA CGGCGGTGGC TACGACTCGG CCAACTCGCT GGAGGCGGGC 
CAGTATGCCG CCCACCTGCC GGCGGTGCTG CCCGAGATGC ATGGCCATGG ATTCGCCACC 
GATCCCTACC AGACGGCCGG CTATGGCGGT GGCAATACGG GCGGAGGCAG TGCATCCAAA 
TCGGAACTGG ACTATGGCGG CAGCTACAAT CAGGCGTGGT CGAATGGCTA TCAGAACTAT 
CAGTATGGCA GTTGTTTGGC CACCGCCCAG TACGGTCCTC AGGCGGCGCC GCCACCACAG 
CCACCGCCTC CGCCGCCGGT GGTGCTGTGC CCGCAGCTGT ACTCCACGGT CAATCAGAAT 
CAAATCCACC TGCATCTGCA CAGCAGCGAG AAGCTGGAGC AGTATCTGGG CACGGCCACT 
AGTGCGGACC ATCTCACCAT TGGATCCCTG ACGGGCAGCA GTCGTTCCAG CATTGAAATT 
GGCCAGGATC AGTACCACCA GCAGGTGCAC CACGCACAGC AGCAGCAACA GCAGCAGCAG 
CAACAACAAC AGGTGCACCA CCCACAGCAG CAGCAGGTGG AATCCGCTGG CGAAGTGGGT 
GGCAGTGGTG CCGGCGGAGT GGAATCCGCC AGGGAGGAGG ACGTGGGCGA TCTCTCGCAG 
GTGTGGCGAC CCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096919.1
CDS	223..2340
Translation	MHLHLLAAEKTPPSPSPNPTPTPSASPSGHTGAAGESQLDLASQTESQLQLGLPLASGYGLGLGLGLGLGLGLGQELVADHSTTVAPVSVAGPGRLGRSINGSGGSHHHHHLHHHYSPYHHAHPYHPPHPHAPHHHHHHHPPYPYPPAGPHPPAMVTSSSTSPTGNGWSSSTGDFKGITAVATATGGGVGGATQGATASTGATAAEVLAVSSSASVGSSSPTGGASNGTAHSGHSGHTGGHSSSTASNNNNNGASNSNSNNNNAVHQDLLWMERLVQKRQQEHPGELVRTSNPYFLCSALPAHWRSNKTLPMAFKVVALAEVGDGTYVTIRAGNDENCCAELRNFTTQMKNDVAKFNDLRFVGRSGRGKSFTLTITVATSPPQVATYAKAIKVTVDGPREPRSKTTAEMMGAGEWTNGSSSSAAAYYHSHAHHPHAHHAHAHLQHQMALPPPPPPPAAAPVSVGVGGNGATMGMGMGVGVGMGMNHYGGGYDSANSLEAGQYAAHLPAVLPEMHGHGFATDPYQTAGYGGGNTGGGSASKSELDYGGSYNQAWSNGYQNYQYGSCLATAQYGPQAAPPPQPPPPPPVVLCPQLYSTVNQNQIHLHLHSSEKLEQYLGTATSADHLTIGSLTGSSRSSIEIGQDQYHQQVHHAQQQQQQQQQQQQVHHPQQQQVESAGEVGGSGAGGVESAREEDVGDLSQVWRPY

Target ORF information:

RefSeq Version	NM_001103449.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lozenge (lz), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103449.2

ORF Insert Sequence:

ATGCATTTGC ATCTCCTGGC GGCAGAGAAG ACGCCTCCAT CTCCATCTCC AAATCCAACT 
CCAACTCCAT CAGCATCTCC ATCTGGGCAT ACGGGCGCTG CTGGGGAGTC GCAATTGGAC 
TTGGCATCGC AAACGGAATC GCAATTGCAG CTGGGCTTGC CATTGGCTTC CGGATACGGA 
CTCGGACTCG GATTGGGACT GGGCTTGGGA TTGGGACTGG GCCAGGAATT GGTGGCGGAC 
CACAGCACCA CTGTGGCGCC CGTCAGCGTT GCCGGTCCCG GCCGCCTGGG TCGCAGCATC 
AACGGCAGCG GCGGCAGCCA CCACCATCAC CACCTGCACC ACCACTACTC ACCGTACCAC 
CACGCCCACC CCTACCACCC ACCGCATCCG CACGCGCCCC ACCACCACCA CCACCACCAC 
CCACCGTATC CGTATCCGCC GGCAGGACCA CATCCGCCGG CCATGGTCAC ATCGTCATCC 
ACATCGCCGA CGGGCAACGG CTGGAGCAGC TCCACCGGCG ACTTCAAGGG CATCACAGCG 
GTGGCGACGG CCACTGGCGG CGGCGTAGGT GGTGCCACCC AGGGCGCCAC TGCATCCACG 
GGCGCCACAG CCGCTGAGGT TTTGGCCGTT TCGAGTAGTG CCAGTGTTGG CAGCAGCTCG 
CCCACAGGTG GCGCCAGCAA TGGTACAGCC CATAGTGGGC ATAGTGGACA CACCGGCGGT 
CACAGCAGTA GTACCGCCAG CAACAACAAC AACAACGGTG CCAGCAATAG CAATAGCAAC 
AATAACAACG CCGTTCACCA GGATCTATTG TGGATGGAGC GATTGGTACA GAAGCGACAA 
CAGGAACATC CCGGCGAATT GGTGCGCACG AGCAATCCGT ACTTCCTGTG CTCCGCCCTG 
CCCGCCCATT GGCGCTCCAA CAAGACGCTG CCGATGGCCT TCAAGGTCGT CGCTCTGGCG 
GAAGTGGGCG ATGGCACCTA TGTCACCATC CGGGCGGGAA ACGATGAGAA TTGCTGCGCG 
GAGCTGCGCA ACTTCACCAC CCAGATGAAG AACGACGTGG CCAAGTTCAA TGATCTGCGT 
TTCGTGGGAC GCAGCGGAAG AGGGAAGAGC TTCACGCTGA CCATCACGGT GGCCACGAGT 
CCGCCACAGG TGGCAACCTA CGCCAAAGCC ATCAAGGTGA CCGTCGATGG ACCCCGCGAG 
CCCCGCTCCA AGACAACAGC CGAAATGATG GGCGCCGGCG AATGGACAAA TGGATCCTCG 
AGTTCGGCGG CTGCCTACTA CCACAGCCAC GCCCATCATC CGCACGCCCA CCACGCCCAC 
GCGCACCTGC AACACCAGAT GGCCTTGCCA CCGCCGCCGC CGCCACCCGC TGCGGCACCC 
GTTTCGGTGG GCGTGGGCGG CAATGGAGCC ACCATGGGCA TGGGCATGGG AGTGGGAGTG 
GGCATGGGCA TGAATCACTA CGGCGGTGGC TACGACTCGG CCAACTCGCT GGAGGCGGGC 
CAGTATGCCG CCCACCTGCC GGCGGTGCTG CCCGAGATGC ATGGCCATGG ATTCGCCACC 
GATCCCTACC AGACGGCCGG CTATGGCGGT GGCAATACGG GCGGAGGCAG TGCATCCAAA 
TCGGAACTGG ACTATGGCGG CAGCTACAAT CAGGCGTGGT CGAATGGCTA TCAGAACTAT 
CAGTATGGCA GTTGTTTGGC CACCGCCCAG TACGGTCCTC AGGCGGCGCC GCCACCACAG 
CCACCGCCTC CGCCGCCGGT GGTGCTGTGC CCGCAGCTGT ACTCCACGGT CAATCAGAAT 
CAAATCCACC TGCATCTGCA CAGCAGCGAG AAGCTGGAGC AGTATCTGGG CACGGCCACT 
AGTGCGGACC ATCTCACCAT TGGATCCCTG ACGGGCAGCA GTCGTTCCAG CATTGAAATT 
GGCCAGGATC AGTACCACCA GCAGGTGCAC CACGCACAGC AGCAGCAACA GCAGCAGCAG 
CAACAACAAC AGGTGCACCA CCCACAGCAG CAGCAGGTGG AATCCGCTGG CGAAGTGGGT 
GGCAGTGGTG CCGGCGGAGT GGAATCCGCC AGGGAGGAGG ACGTGGGCGA TCTCTCGCAG 
GTGTGGCGAC CCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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lz cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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