CG31296 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG31296
Entrez Gene ID	318668
Full Name	CG31296 gene product from transcript CG31296-RB
Synonyms	Dmel\CG31296,NEST:bs01d08
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 88F7-88F7

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275679.1	NP_001262608.1	uncharacterized protein, isoform B
NM_169664.3	NP_732044.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ag5r	NP_001245671.1
Drosophila melanogaster (fruit fly)	CG17974	NP_611582.1
Drosophila melanogaster (fruit fly)	scpr-B	NP_650061.1
Drosophila melanogaster (fruit fly)	CG3640	NP_611950.1
Drosophila melanogaster (fruit fly)	scpr-C	NP_650053.1
Drosophila melanogaster (fruit fly)	CG31296	NP_732044.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000485	XP_310606.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004893	XP_314286.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006418	XP_316454.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000356	XP_310756.4
Drosophila melanogaster (fruit fly)	CG9822	NP_611581.1
Drosophila melanogaster (fruit fly)	Ag5r2	NP_524689.2
Drosophila melanogaster (fruit fly)	CG32679	NP_727412.2
Drosophila melanogaster (fruit fly)	CG17575	NP_610821.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013192	XP_003436455.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010550	XP_314523.4
Drosophila melanogaster (fruit fly)	scpr-A	NP_650062.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008109	XP_317349.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006421	XP_316459.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006443	XP_316479.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006420	XP_316457.2
Drosophila melanogaster (fruit fly)	CG42564	NP_649651.2
Drosophila melanogaster (fruit fly)	CG30488	NP_001246284.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006419	XP_316455.3
Drosophila melanogaster (fruit fly)	CG42780	NP_001188554.1
Drosophila melanogaster (fruit fly)	CG6628	NP_648386.1
Drosophila melanogaster (fruit fly)	CG30486	NP_725234.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003354	XP_314254.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006417	XP_316453.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG31296 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG31296 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07147	NM_001275679.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31296), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa07147	NM_169664.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31296), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG31296 ( NM_001275679.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262608.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG31296), partial mRNA.

Price & Availability↑

CloneID	OFa07147
Clone ID Related Accession (Same CDS sequence)	NM_001275679.1 , NM_169664.3
Accession Version	NM_001275679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGAACTAA GTGGGTTCAT ATTCGTAGCA CTGCTCAATT TCATTCCCTT TGGATGCAGC 
AAGTTGGTTG ATTTTTGCCA GCTGCCTTAC TGCGGTACAA ATAACCTAGC CTGTAATAAC 
CCATCGAAAT TCAGCGTCAT GTGTCCACCG AATGCCAGAA CTCTTTCCAT GTCGACCTAC 
AGGAATTTAT TACTAATTGC CTTCAATGAG TTTCGCAATT ATACGGCGAG CGGGAAGCAA 
AAGTATCTCA AGGCAGCGGC GGCACGAATG TCCCGATTGA GTTACTCAAT GGAACTGGAG 
GACTTAGCCC GACTGGCGGT CATCACGTGC TCCACGCACA AGTTTTGCCT GAACTCACAG 
GAGTTCTATT ACGTGGGTAC CAATATAGGT TCCACCCACT ATTTGGGTAA CTTGAACGAC 
TACGAGGACC TAGAACTCAT GTTGCGGATA ATACAGCATT GGACCAGATA TGCGGATTAT 
GTCAACATTA AAATGGGTGT CTATATGCCG ACAACTTTGG GGAAAAGTGG AATAGCAAAA 
GCCCTACTAC TGATGGCAGA TCGGAACACC CATGTGGGCT GTTCGGCTAT GAGATTCACC 
GTGCACTCCG TCCACAACTT CGTCTTCTTG TGCGCCTTCA GCACCGATCT CTTCGTGGAG 
CGACCCATCT ACCGGATGTC TATGAGACCA GGAGCTGCTT GCAAGCGCTT GGATCCCACA 
TACTCAGCAC TTTGTGCTGT TGGTGAAAAC TACGAAAACA ATAAGCCAAT GCTAAATGCA 
AGGGTATTCC AGTTACCCCT CGATTTATCC ATCGGTCGAC AAGCTTATGT GCCAGCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262608.1
CDS	1..843
Translation	MELSGFIFVALLNFIPFGCSKLVDFCQLPYCGTNNLACNNPSKFSVMCPPNARTLSMSTYRNLLLIAFNEFRNYTASGKQKYLKAAAARMSRLSYSMELEDLARLAVITCSTHKFCLNSQEFYYVGTNIGSTHYLGNLNDYEDLELMLRIIQHWTRYADYVNIKMGVYMPTTLGKSGIAKALLLMADRNTHVGCSAMRFTVHSVHNFVFLCAFSTDLFVERPIYRMSMRPGAACKRLDPTYSALCAVGENYENNKPMLNARVFQLPLDLSIGRQAYVPAI

Target ORF information:

RefSeq Version	NM_001275679.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31296), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275679.1

ORF Insert Sequence:

ATGGAACTAA GTGGGTTCAT ATTCGTAGCA CTGCTCAATT TCATTCCCTT TGGATGCAGC 
AAGTTGGTTG ATTTTTGCCA GCTGCCTTAC TGCGGTACAA ATAACCTAGC CTGTAATAAC 
CCATCGAAAT TCAGCGTCAT GTGTCCACCG AATGCCAGAA CTCTTTCCAT GTCGACCTAC 
AGGAATTTAT TACTAATTGC CTTCAATGAG TTTCGCAATT ATACGGCGAG CGGGAAGCAA 
AAGTATCTCA AGGCAGCGGC GGCACGAATG TCCCGATTGA GTTACTCAAT GGAACTGGAG 
GACTTAGCCC GACTGGCGGT CATCACGTGC TCCACGCACA AGTTTTGCCT GAACTCACAG 
GAGTTCTATT ACGTGGGTAC CAATATAGGT TCCACCCACT ATTTGGGTAA CTTGAACGAC 
TACGAGGACC TAGAACTCAT GTTGCGGATA ATACAGCATT GGACCAGATA TGCGGATTAT 
GTCAACATTA AAATGGGTGT CTATATGCCG ACAACTTTGG GGAAAAGTGG AATAGCAAAA 
GCCCTACTAC TGATGGCAGA TCGGAACACC CATGTGGGCT GTTCGGCTAT GAGATTCACC 
GTGCACTCCG TCCACAACTT CGTCTTCTTG TGCGCCTTCA GCACCGATCT CTTCGTGGAG 
CGACCCATCT ACCGGATGTC TATGAGACCA GGAGCTGCTT GCAAGCGCTT GGATCCCACA 
TACTCAGCAC TTTGTGCTGT TGGTGAAAAC TACGAAAACA ATAAGCCAAT GCTAAATGCA 
AGGGTATTCC AGTTACCCCT CGATTTATCC ATCGGTCGAC AAGCTTATGT GCCAGCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31296 ( NM_169664.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732044.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG31296), partial mRNA.

Price & Availability↑

CloneID	OFa07147
Clone ID Related Accession (Same CDS sequence)	NM_001275679.1 , NM_169664.3
Accession Version	NM_169664.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169664.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGGAACTAA GTGGGTTCAT ATTCGTAGCA CTGCTCAATT TCATTCCCTT TGGATGCAGC 
AAGTTGGTTG ATTTTTGCCA GCTGCCTTAC TGCGGTACAA ATAACCTAGC CTGTAATAAC 
CCATCGAAAT TCAGCGTCAT GTGTCCACCG AATGCCAGAA CTCTTTCCAT GTCGACCTAC 
AGGAATTTAT TACTAATTGC CTTCAATGAG TTTCGCAATT ATACGGCGAG CGGGAAGCAA 
AAGTATCTCA AGGCAGCGGC GGCACGAATG TCCCGATTGA GTTACTCAAT GGAACTGGAG 
GACTTAGCCC GACTGGCGGT CATCACGTGC TCCACGCACA AGTTTTGCCT GAACTCACAG 
GAGTTCTATT ACGTGGGTAC CAATATAGGT TCCACCCACT ATTTGGGTAA CTTGAACGAC 
TACGAGGACC TAGAACTCAT GTTGCGGATA ATACAGCATT GGACCAGATA TGCGGATTAT 
GTCAACATTA AAATGGGTGT CTATATGCCG ACAACTTTGG GGAAAAGTGG AATAGCAAAA 
GCCCTACTAC TGATGGCAGA TCGGAACACC CATGTGGGCT GTTCGGCTAT GAGATTCACC 
GTGCACTCCG TCCACAACTT CGTCTTCTTG TGCGCCTTCA GCACCGATCT CTTCGTGGAG 
CGACCCATCT ACCGGATGTC TATGAGACCA GGAGCTGCTT GCAAGCGCTT GGATCCCACA 
TACTCAGCAC TTTGTGCTGT TGGTGAAAAC TACGAAAACA ATAAGCCAAT GCTAAATGCA 
AGGGTATTCC AGTTACCCCT CGATTTATCC ATCGGTCGAC AAGCTTATGT GCCAGCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732044.2
CDS	1..843
Translation	MELSGFIFVALLNFIPFGCSKLVDFCQLPYCGTNNLACNNPSKFSVMCPPNARTLSMSTYRNLLLIAFNEFRNYTASGKQKYLKAAAARMSRLSYSMELEDLARLAVITCSTHKFCLNSQEFYYVGTNIGSTHYLGNLNDYEDLELMLRIIQHWTRYADYVNIKMGVYMPTTLGKSGIAKALLLMADRNTHVGCSAMRFTVHSVHNFVFLCAFSTDLFVERPIYRMSMRPGAACKRLDPTYSALCAVGENYENNKPMLNARVFQLPLDLSIGRQAYVPAI

Target ORF information:

RefSeq Version	NM_169664.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31296), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169664.3

ORF Insert Sequence:

ATGGAACTAA GTGGGTTCAT ATTCGTAGCA CTGCTCAATT TCATTCCCTT TGGATGCAGC 
AAGTTGGTTG ATTTTTGCCA GCTGCCTTAC TGCGGTACAA ATAACCTAGC CTGTAATAAC 
CCATCGAAAT TCAGCGTCAT GTGTCCACCG AATGCCAGAA CTCTTTCCAT GTCGACCTAC 
AGGAATTTAT TACTAATTGC CTTCAATGAG TTTCGCAATT ATACGGCGAG CGGGAAGCAA 
AAGTATCTCA AGGCAGCGGC GGCACGAATG TCCCGATTGA GTTACTCAAT GGAACTGGAG 
GACTTAGCCC GACTGGCGGT CATCACGTGC TCCACGCACA AGTTTTGCCT GAACTCACAG 
GAGTTCTATT ACGTGGGTAC CAATATAGGT TCCACCCACT ATTTGGGTAA CTTGAACGAC 
TACGAGGACC TAGAACTCAT GTTGCGGATA ATACAGCATT GGACCAGATA TGCGGATTAT 
GTCAACATTA AAATGGGTGT CTATATGCCG ACAACTTTGG GGAAAAGTGG AATAGCAAAA 
GCCCTACTAC TGATGGCAGA TCGGAACACC CATGTGGGCT GTTCGGCTAT GAGATTCACC 
GTGCACTCCG TCCACAACTT CGTCTTCTTG TGCGCCTTCA GCACCGATCT CTTCGTGGAG 
CGACCCATCT ACCGGATGTC TATGAGACCA GGAGCTGCTT GCAAGCGCTT GGATCCCACA 
TACTCAGCAC TTTGTGCTGT TGGTGAAAAC TACGAAAACA ATAAGCCAAT GCTAAATGCA 
AGGGTATTCC AGTTACCCCT CGATTTATCC ATCGGTCGAC AAGCTTATGT GCCAGCCATA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG31296 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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