spdo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spdo
Entrez Gene ID	318559
Full Name	sanpodo
Synonyms	0970/02,BcDNA:RE23355,CG31020,Dmel\CG31020,Spdo
General protein information	Preferred Names sanpodo Names CG31020 gene product from transcript CG31020-RA CG31020-PA spdo-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99F6-99F6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170493.3	NP_733372.1	sanpodo

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	spdo	NP_733372.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002689	XP_312234.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spdo is sorted toward late endosomes in a Numb-dependent manner.
Title: A fluorescent tagging approach in Drosophila reveals late endosomal trafficking of Notch and Sanpodo.

Sanpodo suppresses Notch signaling by driving Notch receptor internalization.
Title: Sanpodo controls sensory organ precursor fate by directing Notch trafficking and binding ?-secretase.

In numb mutant cells, internalized Spdo localizes at the basolateral interface of the pIIa/pIIb cells.
Title: Numb inhibits the recycling of Sanpodo in Drosophila sensory organ precursor.

It was shown that the AP-1 complex causes apical accumulation of the Notch activator Sanpodo and stabilization of both Sanpodo and Notch at the interface between sense organ precursor daughter cells.
Title: AP-1 controls the trafficking of Notch and Sanpodo toward E-cadherin junctions in sensory organ precursors.

Sanpodo contains an evolutionarily conserved motif that has been linked to Numb-dependent regulation in vertebrates; Numb regulates Notch signaling independently of Sanpodo membrane trafficking in neural progenitor cells
Title: Numb independently antagonizes Sanpodo membrane targeting and Notch signaling in Drosophila sensory organ precursor cells.

The following spdo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spdo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07089	NM_170493.3 Latest version!	Drosophila melanogaster sanpodo (spdo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spdo ( NM_170493.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733372.1 Drosophila melanogaster sanpodo (spdo), mRNA.

Price & Availability↑

CloneID	OFa07089
Clone ID Related Accession (Same CDS sequence)	NM_170493.3
Accession Version	NM_170493.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sanpodo
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170493.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCAGC ATAACTATAC GAACCCGGCC TTCTGCCAGA ACAGCGAGTA TTACCCGGTG 
CCCAGCGGCA ACTCGTCGCG AGTGGAGTCC ATCTACAACA GAAGCCTCCA GATGAACTTT 
GGAGCGGCTC CTCCGCGCGC CACTCGAGAT CCCCGCGAGG GTCCACTGCG AATGCAGCGT 
AGCATGTCCA CGCGATCGGT GACGCGGAAA CAACACCAGC AGCAGCAGCA GCAGCAACAC 
CAACAGCAGA TACACCAGCA ACACCTGGCT CAGCAGCAGC AACACCAGCC GCAATCCTCT 
AGTGGTCGCT ATGCCTTGGT TCCCCTAGAG GAACTGAACA GCAGTAGGGC CAGTAGATAC 
GCCATAGTTC CAGGACAGGC GGCACAGAGA CTGGCCCGAT CACAGGATAA TCTGGATCGC 
TATGGCTCCA GACATGGATT GCACGAAGAG GAGGAGCCTC ACTCCTTCCA CGAGGAACCC 
CATCAGGAGA CCCAGTTTGC CAGCTTGCCA CCGATACTTA ATTTGCCGCC CAAGCCCAGG 
AATATTAACA ACAATACCAA TGCCAATCAA ACCCAGATTA AGCATGCTTT TAGTAGCGAC 
TTCGGCAGCA AGACCTTCTT GATAGTGGAT AAGAATAGCA ACCAGAGGTA CCAGATGGTG 
CCCACCGCCG AGGACGAGGA GCTGGTAGAC GAGAACCAGG AGATCATACA GATGCACAAT 
GGCCGCGCCC ATCGATATGC AGTGGTGCCC ACGGATGCGG ACGACGATGA TCTGGCGGAG 
GAGCAGGAGC AGCAGGAAAC CTGCCTGAGC AACATGGAAT TGAGCAGGCA GTACGCAGCG 
AGGACCTCAA CGCCGCAGCA AAAGAATGCG GCTGCCGCTA CGAGGGCCCT GCACGAGCTC 
CTGACCACGC CCAGGAAGAT GCAGCCGCCT CCGCGATTGG CGCACTCAAC GCCCCGGAAC 
GCCGCCCACC ACGAGCAGCT GCAGCTGGAA AGGCGCTTGG AGATCTACAA AAGCCAACAG 
ATCTACCAAA GCCAACAAGT TGGAGCGGTA GTAGGAGTCC CTCCAACCTT GCCACTTCGC 
CAGAATCGAC TCTCCCCCCA GAGACTTCAC TACGAGACCG TGAAGCCGCT TCCCATGCAG 
ATCGCCGATC GATCCATGGC CGTCATCAGT CCCAGGGTCC AGGAGCAGGG GCAGGAGCAG 
GAACGGGAAC AGGACATGAG CAGCATCCAG GGAAAGGGCA AGAAAAACAG CTCCTATCCG 
CAGAAGATGG CCAATGCCAC CATTACCTTG GCCGTTGTCT CCCTGATGCT CGTCTTGGGC 
AGCACCATGA ATGCGGGGCT CATCATCTAC ATGATAGCAC ACCTCGGAAA GTCCTTTTAC 
CTGCAGTTTA GCCTGGTGGC CTCCTTCTCC GGAATGGGAC TCGGCTTCCT GGGATTCAAG 
TCGCGCCACT GCGAGTGGCT GCCGAACAGG AGCTATAGTT CCGGCTATAT CCTGGTCACC 
GTATTTTGCC TATTCAAGTC CTGCGGATTG CTTGTGATAC TCGTGGTGGA CCCCTTCCCC 
GGATTTCCTG TCCACGATGT GACCACTGGC GTCATCCTTG GACTGTCGAC TTTCACCATG 
TTCTTCATCG GATTGGGAGT GCTGGGCAGC TTGTGGTGCC ACCGACCTCC GCCAGACAAC 
CGCGTCAACG TGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733372.1
CDS	130..1827
Translation	MSQHNYTNPAFCQNSEYYPVPSGNSSRVESIYNRSLQMNFGAAPPRATRDPREGPLRMQRSMSTRSVTRKQHQQQQQQQHQQQIHQQHLAQQQQHQPQSSSGRYALVPLEELNSSRASRYAIVPGQAAQRLARSQDNLDRYGSRHGLHEEEEPHSFHEEPHQETQFASLPPILNLPPKPRNINNNTNANQTQIKHAFSSDFGSKTFLIVDKNSNQRYQMVPTAEDEELVDENQEIIQMHNGRAHRYAVVPTDADDDDLAEEQEQQETCLSNMELSRQYAARTSTPQQKNAAAATRALHELLTTPRKMQPPPRLAHSTPRNAAHHEQLQLERRLEIYKSQQIYQSQQVGAVVGVPPTLPLRQNRLSPQRLHYETVKPLPMQIADRSMAVISPRVQEQGQEQEREQDMSSIQGKGKKNSSYPQKMANATITLAVVSLMLVLGSTMNAGLIIYMIAHLGKSFYLQFSLVASFSGMGLGFLGFKSRHCEWLPNRSYSSGYILVTVFCLFKSCGLLVILVVDPFPGFPVHDVTTGVILGLSTFTMFFIGLGVLGSLWCHRPPPDNRVNVV

Target ORF information:

RefSeq Version	NM_170493.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sanpodo (spdo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170493.3

ORF Insert Sequence:

ATGTCGCAGC ATAACTATAC GAACCCGGCC TTCTGCCAGA ACAGCGAGTA TTACCCGGTG 
CCCAGCGGCA ACTCGTCGCG AGTGGAGTCC ATCTACAACA GAAGCCTCCA GATGAACTTT 
GGAGCGGCTC CTCCGCGCGC CACTCGAGAT CCCCGCGAGG GTCCACTGCG AATGCAGCGT 
AGCATGTCCA CGCGATCGGT GACGCGGAAA CAACACCAGC AGCAGCAGCA GCAGCAACAC 
CAACAGCAGA TACACCAGCA ACACCTGGCT CAGCAGCAGC AACACCAGCC GCAATCCTCT 
AGTGGTCGCT ATGCCTTGGT TCCCCTAGAG GAACTGAACA GCAGTAGGGC CAGTAGATAC 
GCCATAGTTC CAGGACAGGC GGCACAGAGA CTGGCCCGAT CACAGGATAA TCTGGATCGC 
TATGGCTCCA GACATGGATT GCACGAAGAG GAGGAGCCTC ACTCCTTCCA CGAGGAACCC 
CATCAGGAGA CCCAGTTTGC CAGCTTGCCA CCGATACTTA ATTTGCCGCC CAAGCCCAGG 
AATATTAACA ACAATACCAA TGCCAATCAA ACCCAGATTA AGCATGCTTT TAGTAGCGAC 
TTCGGCAGCA AGACCTTCTT GATAGTGGAT AAGAATAGCA ACCAGAGGTA CCAGATGGTG 
CCCACCGCCG AGGACGAGGA GCTGGTAGAC GAGAACCAGG AGATCATACA GATGCACAAT 
GGCCGCGCCC ATCGATATGC AGTGGTGCCC ACGGATGCGG ACGACGATGA TCTGGCGGAG 
GAGCAGGAGC AGCAGGAAAC CTGCCTGAGC AACATGGAAT TGAGCAGGCA GTACGCAGCG 
AGGACCTCAA CGCCGCAGCA AAAGAATGCG GCTGCCGCTA CGAGGGCCCT GCACGAGCTC 
CTGACCACGC CCAGGAAGAT GCAGCCGCCT CCGCGATTGG CGCACTCAAC GCCCCGGAAC 
GCCGCCCACC ACGAGCAGCT GCAGCTGGAA AGGCGCTTGG AGATCTACAA AAGCCAACAG 
ATCTACCAAA GCCAACAAGT TGGAGCGGTA GTAGGAGTCC CTCCAACCTT GCCACTTCGC 
CAGAATCGAC TCTCCCCCCA GAGACTTCAC TACGAGACCG TGAAGCCGCT TCCCATGCAG 
ATCGCCGATC GATCCATGGC CGTCATCAGT CCCAGGGTCC AGGAGCAGGG GCAGGAGCAG 
GAACGGGAAC AGGACATGAG CAGCATCCAG GGAAAGGGCA AGAAAAACAG CTCCTATCCG 
CAGAAGATGG CCAATGCCAC CATTACCTTG GCCGTTGTCT CCCTGATGCT CGTCTTGGGC 
AGCACCATGA ATGCGGGGCT CATCATCTAC ATGATAGCAC ACCTCGGAAA GTCCTTTTAC 
CTGCAGTTTA GCCTGGTGGC CTCCTTCTCC GGAATGGGAC TCGGCTTCCT GGGATTCAAG 
TCGCGCCACT GCGAGTGGCT GCCGAACAGG AGCTATAGTT CCGGCTATAT CCTGGTCACC 
GTATTTTGCC TATTCAAGTC CTGCGGATTG CTTGTGATAC TCGTGGTGGA CCCCTTCCCC 
GGATTTCCTG TCCACGATGT GACCACTGGC GTCATCCTTG GACTGTCGAC TTTCACCATG 
TTCTTCATCG GATTGGGAGT GCTGGGCAGC TTGTGGTGCC ACCGACCTCC GCCAGACAAC 
CGCGTCAACG TGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spdo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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