fh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fh
Entrez Gene ID	31845
Full Name	frataxin
Synonyms	BcDNA:AT09528,CG8971,DFH,Dfh,Dmel\CG8971,X25,dfh
General protein information	Preferred Names frataxin Names CG8971 gene product from transcript CG8971-RA CG8971-PA CG8971-PB fh-PA fh-PB frataxin homolog frataxin homologue frataxin-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8C14-8C14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272444.1	NP_001259373.1	frataxin, isoform B
NM_078539.3	NP_511094.1	frataxin

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	frh-1	NP_495183.1
Drosophila melanogaster (fruit fly)	fh	NP_511094.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000813	XP_311351.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors show that loss of frataxin homolog (fh) in Drosophila leads to iron toxicity, which in turn induces sphingolipid synthesis and ectopically activates 3-phosphoinositide dependent protein kinase-1 (Pdk1) and myocyte enhancer factor-2 (Mef2).
Title: Loss of Frataxin induces iron toxicity, sphingolipid synthesis, and Pdk1/Mef2 activation, leading to neurodegeneration.

Frataxin overexpression in the nervous system reduces life span, impairs locomotor ability and causes brain degeneration.
Title: Overexpression of human and fly frataxins in Drosophila provokes deleterious effects at biochemical, physiological and developmental levels.

Defects in axonal transport of mitochondria appear late in development in distal nerve of larvae showing reduced frataxin expression, with retrograde movement preferentially affected.
Title: Defects in mitochondrial axonal transport and membrane potential without increased reactive oxygen species production in a Drosophila model of Friedreich ataxia.

Strong involvement of glial cells and lipid peroxidation in the generation of Friedreich's ataxia.
Title: Altered lipid metabolism in a Drosophila model of Friedreich's ataxia.

Dfh-assisted assembly of Fe-S clusters occurs with an observed kinetic rate constant ( k obs) of 0.096 min (-1)
Title: Drosophila frataxin: an iron chaperone during cellular Fe-S cluster bioassembly.

The following fh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01170	NM_001272444.1 Latest version!	Drosophila melanogaster frataxin (fh), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa03322	NM_078539.3 Latest version!	Drosophila melanogaster frataxin (fh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fh ( NM_001272444.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259373.1 Drosophila melanogaster frataxin (fh), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01170
Clone ID Related Accession (Same CDS sequence)	NM_001272444.1
Accession Version	NM_001272444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-15
Organism	Drosophila melanogaster(fruit fly)
Product	frataxin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.18 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCCG GTCGTTTGAT GGTCCGTTCG ATCGTTGGTC GGGCATGCTT GGCCACCATG 
GGCAGGTGGT CAAAGCCCCA AGCACACGCC AGCCAAGTGA TCCTGCCCAG CACACCAGCG 
ATAGCCGCAG TTGCTATTCA ATGCGAGGAA TTCACTGCCA ACCGGCGATT GTTTAGCAGT 
CAAATTGAGA CGGAATCCAC ATTGGACGGC GCCACCTACG AGCGTGTGTG CTCCGACACC 
CTGGACGCAC TGTGCGACTA CTTCGAGGAG CTGACGGAGA ACGCCTCCGA GCTGCAGGGC 
ACGGATGTGG CTTACAGCGT AGGATGGCGT GCTAACCGTG AACCTGGGAG GACAACACGG 
CACCTATGTG ATCAACCGGC AGACGCCCAA CAAGCAGATC TGGCTCAGTT CGCCCACCAG 
CGGTCCCAAG CGATACGATT TCGTCGGCAC TGTGGCGGCG GGCAGATGGA TCTACAAGCA 
CAGTGGTCAG TCGCTGCACG AACTGTTGCA GCAGGAGATA CCCGGCATAC TGAAGTCACA 
GTCCGTGGAC TTCCTACGCC TGCCCTACTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259373.1
CDS	60..632
Translation	MFAGRLMVRSIVGRACLATMGRWSKPQAHASQVILPSTPAIAAVAIQCEEFTANRRLFSSQIETESTLDGATYERVCSDTLDALCDYFEELTENASELQGTDVAYSVGWRANREPGRTTRHLCDQPADAQQADLAQFAHQRSQAIRFRRHCGGGQMDLQAQWSVAARTVAAGDTRHTEVTVRGLPTPALL

Target ORF information:

RefSeq Version	NM_001272444.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frataxin (fh), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272444.1

ORF Insert Sequence:

ATGTTTGCCG GTCGTTTGAT GGTCCGTTCG ATCGTTGGTC GGGCATGCTT GGCCACCATG 
GGCAGGTGGT CAAAGCCCCA AGCACACGCC AGCCAAGTGA TCCTGCCCAG CACACCAGCG 
ATAGCCGCAG TTGCTATTCA ATGCGAGGAA TTCACTGCCA ACCGGCGATT GTTTAGCAGT 
CAAATTGAGA CGGAATCCAC ATTGGACGGC GCCACCTACG AGCGTGTGTG CTCCGACACC 
CTGGACGCAC TGTGCGACTA CTTCGAGGAG CTGACGGAGA ACGCCTCCGA GCTGCAGGGC 
ACGGATGTGG CTTACAGCGT AGGATGGCGT GCTAACCGTG AACCTGGGAG GACAACACGG 
CACCTATGTG ATCAACCGGC AGACGCCCAA CAAGCAGATC TGGCTCAGTT CGCCCACCAG 
CGGTCCCAAG CGATACGATT TCGTCGGCAC TGTGGCGGCG GGCAGATGGA TCTACAAGCA 
CAGTGGTCAG TCGCTGCACG AACTGTTGCA GCAGGAGATA CCCGGCATAC TGAAGTCACA 
GTCCGTGGAC TTCCTACGCC TGCCCTACTG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fh ( NM_078539.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511094.1 Drosophila melanogaster frataxin (fh), mRNA.

Price & Availability↑

CloneID	OFa03322
Clone ID Related Accession (Same CDS sequence)	NM_078539.3
Accession Version	NM_078539.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frataxin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078539.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGCCG GTCGTTTGAT GGTCCGTTCG ATCGTTGGTC GGGCATGCTT GGCCACCATG 
GGCAGGTGGT CAAAGCCCCA AGCACACGCC AGCCAAGTGA TCCTGCCCAG CACACCAGCG 
ATAGCCGCAG TTGCTATTCA ATGCGAGGAA TTCACTGCCA ACCGGCGATT GTTTAGCAGT 
CAAATTGAGA CGGAATCCAC ATTGGACGGC GCCACCTACG AGCGTGTGTG CTCCGACACC 
CTGGACGCAC TGTGCGACTA CTTCGAGGAG CTGACGGAGA ACGCCTCCGA GCTGCAGGGC 
ACGGATGTGG CTTACAGCGA TGGCGTGCTA ACCGTGAACC TGGGAGGACA ACACGGCACC 
TATGTGATCA ACCGGCAGAC GCCCAACAAG CAGATCTGGC TCAGTTCGCC CACCAGCGGT 
CCCAAGCGAT ACGATTTCGT CGGCACTGTG GCGGCGGGCA GATGGATCTA CAAGCACAGT 
GGTCAGTCGC TGCACGAACT GTTGCAGCAG GAGATACCCG GCATACTGAA GTCACAGTCC 
GTGGACTTCC TACGCCTGCC CTACTGTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511094.1
CDS	60..632
Translation	MFAGRLMVRSIVGRACLATMGRWSKPQAHASQVILPSTPAIAAVAIQCEEFTANRRLFSSQIETESTLDGATYERVCSDTLDALCDYFEELTENASELQGTDVAYSDGVLTVNLGGQHGTYVINRQTPNKQIWLSSPTSGPKRYDFVGTVAAGRWIYKHSGQSLHELLQQEIPGILKSQSVDFLRLPYCS

Target ORF information:

RefSeq Version	NM_078539.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frataxin (fh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078539.3

ORF Insert Sequence:

ATGTTTGCCG GTCGTTTGAT GGTCCGTTCG ATCGTTGGTC GGGCATGCTT GGCCACCATG 
GGCAGGTGGT CAAAGCCCCA AGCACACGCC AGCCAAGTGA TCCTGCCCAG CACACCAGCG 
ATAGCCGCAG TTGCTATTCA ATGCGAGGAA TTCACTGCCA ACCGGCGATT GTTTAGCAGT 
CAAATTGAGA CGGAATCCAC ATTGGACGGC GCCACCTACG AGCGTGTGTG CTCCGACACC 
CTGGACGCAC TGTGCGACTA CTTCGAGGAG CTGACGGAGA ACGCCTCCGA GCTGCAGGGC 
ACGGATGTGG CTTACAGCGA TGGCGTGCTA ACCGTGAACC TGGGAGGACA ACACGGCACC 
TATGTGATCA ACCGGCAGAC GCCCAACAAG CAGATCTGGC TCAGTTCGCC CACCAGCGGT 
CCCAAGCGAT ACGATTTCGT CGGCACTGTG GCGGCGGGCA GATGGATCTA CAAGCACAGT 
GGTCAGTCGC TGCACGAACT GTTGCAGCAG GAGATACCCG GCATACTGAA GTCACAGTCC 
GTGGACTTCC TACGCCTGCC CTACTGTAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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