CG32686 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32686
Entrez Gene ID	318153
Full Name	CG32686 gene product from transcript CG32686-RA
Synonyms	BcDNA:AT25933,Dmel\CG32686,anon-WO02059370.11
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 9B12-9B12

mRNA and Protein(s)

mRNA	Protein	Name
NM_167227.3	NP_727414.1	uncharacterized protein, isoform A
NM_167226.2	NP_727413.1	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32686 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32686 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02907	NM_167227.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32686), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02909	NM_167226.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32686), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32686 ( NM_167227.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727414.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG32686), mRNA.

Price & Availability↑

CloneID	OFa02907
Clone ID Related Accession (Same CDS sequence)	NM_167227.3
Accession Version	NM_167227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_167227.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGAACG GTGTAAGGAG TAGCATGTCC ACCTCAAGTC CCCACTCGAA CACGACCAGC 
AGCAAGACCA GTGTGATAGA GTGCATCAAT GGGATCAGGA CGTCCAGCAC CGTGCAACAG 
AATCCTGGAA TTCAAGGGGC ATTGGGTAGC AAGTCCATTA AGCTAACCTC AACGGTGATG 
AGGCTCATCC AGCCTGTCGC ATCGAAAGGT GACGTCGAAT CGCCGGAAAT TCCCATGGAG 
AAGGATAACG AGAAGGAGAA GGAGAAGAAA AAGCTGACCG TGCAGAAAGG AAACGCCAAA 
ACTAAGCTGG CCATGAACGC ACCCATGGTC CAATCGATTT CGCCACCAAA GTTCAGTCCC 
ATGGTTAAGG TACCGATACC AATCAGTCAG TCGGTAAATC ATGTCGTCAA TCAGCTAACC 
GCTCAACTGA AGGAAGCTGC CATGATGCAC AAATCGAAAG ACAATCTACC GAAGGATCGT 
GATACGTATG AAGCATTCGG ACCAAAGGAT CCCTTTAGAA AAGCTTCCAT TTATCTAATC 
ATGGATCAGC CAAGAAATCA ATCAGGTGCT TCGACAAACG AACCTACAAA GGATTCGTGG 
GTTATTCAAC CAATAGAGCA ACCAAACTCA TCTACGATTA ATCTGCACAA GAATGAAGTG 
ACGAATCCTT ATTTAGACGA ATCAATTGAA CATCCCAGGC AATATATAAA CCAAAGAACG 
GTGAGAGCGG CAAGTCAGCA GAGAAATGAA TCAAAGAGCC CATCTGTGAA TCATATGATA 
AGCCTTCCAA GGGACTCAAC TGGAGTTAAT CATTCAATGA ATCAGTATGT AATGCCGCCA 
AAGACTAGAA GGCACCCAAT GAGGAATCCA TCGAAAAGCT CTTTGCTTCC GAGGTCCAGA 
GAGTCGAAGG CAGGAATATC AAGGAATCCG TACATTGAAG AGAGCAAAAC TTTATCGAGA 
TTTCCAGCCC GGAGACCACC TTCCGTTTCG GTCAATAGAC CGCGCCAGCC CTCGACATCC 
ACTGGAATCC CTAAAGTTCT GCCTCCCCCA GAACCGCAAA AACCAGAATC TCCAATTGTT 
GGCAAGATCA TTAGGAAGGA ACAGCACAAA GTGCCTCGTA CCATGGAAGA CGGCATCGAT 
ATGAGCTACC AGTACTTCGT GTCTATTCCC CTAAAGCGTG GCAGGAAGCC ACAGGTAGTT 
CGCTACCTTT ACCGCCCAAT GGTGCGTCAA CTTAACGCTC CCACTTCGCC CAATCGACGC 
TCGAGGCGGG CAAGTAGAAA AGCAGCAGCG GCGGCAGCAG CTGCAGCGGC CAGCGAAGAA 
GAGCAGTTAA AGGCAGCGGA GGAGGGATCA CCTAAGATAG ATCCCGAGCT GAAAGCACTG 
GGCGGAGTGC CTGTGCCGCT GGAGGATCCA CCGCAGGTTA AGGAGCCATC ACCACAACCG 
GCAGTTAAGC CAAAGATATT CGTAGATCCC GAGGCAGTTC TAAATGGTCC GTACGAGGTG 
CCACCGCTCA AGCTGGATGC CCGCTACAAG TCGATGATGG AGCAGTTGGC CCAGATGCCA 
TATCCCGAGG ATCGATCATC TCGTGCTCGT CGTCGACGCT CCAATCGCCA AGCTCGCGCT 
GCCGGTGGCG CTGACCAGGT GCCTACATCG GTGCATAGCC TGGGCGTTGG AGACGGTGAC 
GCGCCTAAAA GTGATATGAA CTCCCTGCAG CAGGTGTCCT CCATATGGAA ATCTGGTTCG 
CGTTTGCCAA AACCAGTCAA CTATCAACCA GAACCTAAAC GTCTGTGCAC GGCTACCGGT 
GCTCCCATGT CATTTCATTC TCCGGTGCAA TTCGGACACT CCGTGGCGGA CTCGGACCAC 
TTTATTCTGC CAGATGAGCC AATGATTCAG GCCATAACCT ATGACGACAT CGAACAGACG 
GAGCAAAAGC TACTGGATGA GAAGCCGGTT CAAAAGGAGG AGGAACGCAA AGAGGGAACA 
ACCAAATCAG TGAGCTTTCA TCCTGTCGAG GCAGAAGTAA GTGAGTTCCA CTTGAGCAGC 
AGCTCCATCA GCATCAGCTT AACTTCGAGC AGCAACTGTG GCGTAACCAA GACCAAAACC 
TCTCGCCCCT CCAAGAAGAG GAAGTCCAAG TCCAAGGGAA GGGGCAAGTT GCGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727414.1
CDS	1169..3328
Translation	MENGVRSSMSTSSPHSNTTSSKTSVIECINGIRTSSTVQQNPGIQGALGSKSIKLTSTVMRLIQPVASKGDVESPEIPMEKDNEKEKEKKKLTVQKGNAKTKLAMNAPMVQSISPPKFSPMVKVPIPISQSVNHVVNQLTAQLKEAAMMHKSKDNLPKDRDTYEAFGPKDPFRKASIYLIMDQPRNQSGASTNEPTKDSWVIQPIEQPNSSTINLHKNEVTNPYLDESIEHPRQYINQRTVRAASQQRNESKSPSVNHMISLPRDSTGVNHSMNQYVMPPKTRRHPMRNPSKSSLLPRSRESKAGISRNPYIEESKTLSRFPARRPPSVSVNRPRQPSTSTGIPKVLPPPEPQKPESPIVGKIIRKEQHKVPRTMEDGIDMSYQYFVSIPLKRGRKPQVVRYLYRPMVRQLNAPTSPNRRSRRASRKAAAAAAAAAASEEEQLKAAEEGSPKIDPELKALGGVPVPLEDPPQVKEPSPQPAVKPKIFVDPEAVLNGPYEVPPLKLDARYKSMMEQLAQMPYPEDRSSRARRRRSNRQARAAGGADQVPTSVHSLGVGDGDAPKSDMNSLQQVSSIWKSGSRLPKPVNYQPEPKRLCTATGAPMSFHSPVQFGHSVADSDHFILPDEPMIQAITYDDIEQTEQKLLDEKPVQKEEERKEGTTKSVSFHPVEAEVSEFHLSSSSISISLTSSSNCGVTKTKTSRPSKKRKSKSKGRGKLRK

Target ORF information:

RefSeq Version	NM_167227.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32686), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167227.3

ORF Insert Sequence:

ATGGAGAACG GTGTAAGGAG TAGCATGTCC ACCTCAAGTC CCCACTCGAA CACGACCAGC 
AGCAAGACCA GTGTGATAGA GTGCATCAAT GGGATCAGGA CGTCCAGCAC CGTGCAACAG 
AATCCTGGAA TTCAAGGGGC ATTGGGTAGC AAGTCCATTA AGCTAACCTC AACGGTGATG 
AGGCTCATCC AGCCTGTCGC ATCGAAAGGT GACGTCGAAT CGCCGGAAAT TCCCATGGAG 
AAGGATAACG AGAAGGAGAA GGAGAAGAAA AAGCTGACCG TGCAGAAAGG AAACGCCAAA 
ACTAAGCTGG CCATGAACGC ACCCATGGTC CAATCGATTT CGCCACCAAA GTTCAGTCCC 
ATGGTTAAGG TACCGATACC AATCAGTCAG TCGGTAAATC ATGTCGTCAA TCAGCTAACC 
GCTCAACTGA AGGAAGCTGC CATGATGCAC AAATCGAAAG ACAATCTACC GAAGGATCGT 
GATACGTATG AAGCATTCGG ACCAAAGGAT CCCTTTAGAA AAGCTTCCAT TTATCTAATC 
ATGGATCAGC CAAGAAATCA ATCAGGTGCT TCGACAAACG AACCTACAAA GGATTCGTGG 
GTTATTCAAC CAATAGAGCA ACCAAACTCA TCTACGATTA ATCTGCACAA GAATGAAGTG 
ACGAATCCTT ATTTAGACGA ATCAATTGAA CATCCCAGGC AATATATAAA CCAAAGAACG 
GTGAGAGCGG CAAGTCAGCA GAGAAATGAA TCAAAGAGCC CATCTGTGAA TCATATGATA 
AGCCTTCCAA GGGACTCAAC TGGAGTTAAT CATTCAATGA ATCAGTATGT AATGCCGCCA 
AAGACTAGAA GGCACCCAAT GAGGAATCCA TCGAAAAGCT CTTTGCTTCC GAGGTCCAGA 
GAGTCGAAGG CAGGAATATC AAGGAATCCG TACATTGAAG AGAGCAAAAC TTTATCGAGA 
TTTCCAGCCC GGAGACCACC TTCCGTTTCG GTCAATAGAC CGCGCCAGCC CTCGACATCC 
ACTGGAATCC CTAAAGTTCT GCCTCCCCCA GAACCGCAAA AACCAGAATC TCCAATTGTT 
GGCAAGATCA TTAGGAAGGA ACAGCACAAA GTGCCTCGTA CCATGGAAGA CGGCATCGAT 
ATGAGCTACC AGTACTTCGT GTCTATTCCC CTAAAGCGTG GCAGGAAGCC ACAGGTAGTT 
CGCTACCTTT ACCGCCCAAT GGTGCGTCAA CTTAACGCTC CCACTTCGCC CAATCGACGC 
TCGAGGCGGG CAAGTAGAAA AGCAGCAGCG GCGGCAGCAG CTGCAGCGGC CAGCGAAGAA 
GAGCAGTTAA AGGCAGCGGA GGAGGGATCA CCTAAGATAG ATCCCGAGCT GAAAGCACTG 
GGCGGAGTGC CTGTGCCGCT GGAGGATCCA CCGCAGGTTA AGGAGCCATC ACCACAACCG 
GCAGTTAAGC CAAAGATATT CGTAGATCCC GAGGCAGTTC TAAATGGTCC GTACGAGGTG 
CCACCGCTCA AGCTGGATGC CCGCTACAAG TCGATGATGG AGCAGTTGGC CCAGATGCCA 
TATCCCGAGG ATCGATCATC TCGTGCTCGT CGTCGACGCT CCAATCGCCA AGCTCGCGCT 
GCCGGTGGCG CTGACCAGGT GCCTACATCG GTGCATAGCC TGGGCGTTGG AGACGGTGAC 
GCGCCTAAAA GTGATATGAA CTCCCTGCAG CAGGTGTCCT CCATATGGAA ATCTGGTTCG 
CGTTTGCCAA AACCAGTCAA CTATCAACCA GAACCTAAAC GTCTGTGCAC GGCTACCGGT 
GCTCCCATGT CATTTCATTC TCCGGTGCAA TTCGGACACT CCGTGGCGGA CTCGGACCAC 
TTTATTCTGC CAGATGAGCC AATGATTCAG GCCATAACCT ATGACGACAT CGAACAGACG 
GAGCAAAAGC TACTGGATGA GAAGCCGGTT CAAAAGGAGG AGGAACGCAA AGAGGGAACA 
ACCAAATCAG TGAGCTTTCA TCCTGTCGAG GCAGAAGTAA GTGAGTTCCA CTTGAGCAGC 
AGCTCCATCA GCATCAGCTT AACTTCGAGC AGCAACTGTG GCGTAACCAA GACCAAAACC 
TCTCGCCCCT CCAAGAAGAG GAAGTCCAAG TCCAAGGGAA GGGGCAAGTT GCGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32686 ( NM_167226.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727413.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG32686), mRNA.

Price & Availability↑

CloneID	OFa02909
Clone ID Related Accession (Same CDS sequence)	NM_167226.2
Accession Version	NM_167226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_167226.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTACGG AAATGTCAAA ATTCTTGGGC GGAGGCCGCC GTTATCCAGT AAATCGAAAC 
AAGGCGCTCC AGACCAAAAC AGGAACTGGT GTCGCCGCAA AAGGTGGCGA GCCAACAGCA 
CGTGGTGCTC CATCGAAACC ACCAAGCCAA AGAAATCCAA AGCCCACAAA GGACGCAGAT 
GTACCAGGTG CAGCAGGAGC TTCAAGACTA GGAGGAGGAA CGAGTGCATC ACAGCCGCCA 
GTCAAATCGA ATCCAAGACC ATCGAATGGT GCTAGTGGAC CATCAGCAGC TGGAGCTGTG 
CCCAAGAGAC CACCGAATCG AGCAAGTCCT GCAACTGCGG TGACAGCAGA AGCTGGCAAG 
GCAGTGCCCA AGAGAGCAAC TGGTGGTGGT GTTCCTGCAA GGGGTGTTAT GCAAACCACT 
GCCACATCCA CGTTGCGTCG AAGGCCAGCT CCACCGACTT CTGCAAGGAT ACCCATATCG 
CGGCGCCTAG ACGAGGCTAA GTGGATCATG AAGCCATCGG ATAACTTTGC GCCGTGCAGC 
GAGTCCTCCT CCGAGGATAA TTTCAAGGAA CAGGCCAGCG GATCGCAGGA GCGCAAAGAG 
GATCACGCTG GTGATTTGGA CGGGAGCAGT AGCAGTGGCC ATCTGCACAG GTCGCAGCTA 
CTGATGTCTT TGCCGGCTGA ACAGGTTGGG CGGACAAAGA TCAAGGGACG AACATCGCGC 
ATATTCCGTG TGCCCAGCCA GAGGATCATG CACGTCGATC AGCTGATGCA GGCACAGCTG 
CGCCAGATGG ATAGCCGAAA AACTAGAGCC CAGTCCAGCA GTCGTTCACA TAGTAGGTCC 
CCCAACTGGT CAGCCAGTAG GTTACCAAGT CCATTGTCCC AACAATCATC CTTTAGATCG 
CCAAGCATCT CGACCAGTTC AAACGAAATT GCGGAGGTAA AGGATGTGGC GCTGCGTGAT 
GCCAGAACCG TAACCAAGAT ACCCGCCACC GGGCTGGAAT CCTGCTCTCC CAGTCCGGAC 
GCTAGTCCCG CTACCAGCCA GAGTCGCCTT CAGAGCCCAA CCATTAGCAA CGCTTCCAGC 
TGCTGTACCA TGGAGAACGG TGTAAGGAGT AGCATGTCCA CCTCAAGTCC CCACTCGAAC 
ACGACCAGCA GCAAGACCAG TGTGATAGAG TGCATCAATG GGATCAGGAC GTCCAGCACC 
GTGCAACAGA ATCCTGGAAT TCAAGGGGCA TTGGGTAGCA AGTCCATTAA GCTAACCTCA 
ACGGTGATGA GGCTCATCCA GCCTGTCGCA TCGAAAGGTG ACGTCGAATC GCCGGAAATT 
CCCATGGAGA AGGATAACGA GAAGGAGAAG GAGAAGAAAA AGCTGACCGT GCAGAAAGGA 
AACGCCAAAA CTAAGCTGGC CATGAACGCA CCCATGGTCC AATCGATTTC GCCACCAAAG 
TTCAGTCCCA TGGTTAAGGT ACCGATACCA ATCAGTCAGT CGGTAAATCA TGTCGTCAAT 
CAGCTAACCG CTCAACTGAA GGAAGCTGCC ATGATGCACA AATCGAAAGA CAATCTACCG 
AAGGATCGTG ATACGTATGA AGCATTCGGA CCAAAGGATC CCTTTAGAAA AGCTTCCATT 
TATCTAATCA TGGATCAGCC AAGAAATCAA TCAGGTGCTT CGACAAACGA ACCTACAAAG 
GATTCGTGGG TTATTCAACC AATAGAGCAA CCAAACTCAT CTACGATTAA TCTGCACAAG 
AATGAAGTGA CGAATCCTTA TTTAGACGAA TCAATTGAAC ATCCCAGGCA ATATATAAAC 
CAAAGAACGG TGAGAGCGGC AAGTCAGCAG AGAAATGAAT CAAAGAGCCC ATCTGTGAAT 
CATATGATAA GCCTTCCAAG GGACTCAACT GGAGTTAATC ATTCAATGAA TCAGTATGTA 
ATGCCGCCAA AGACTAGAAG GCACCCAATG AGGAATCCAT CGAAAAGCTC TTTGCTTCCG 
AGGTCCAGAG AGTCGAAGGC AGGAATATCA AGGAATCCGT ACATTGAAGA GAGCAAAACT 
TTATCGAGAT TTCCAGCCCG GAGACCACCT TCCGTTTCGG TCAATAGACC GCGCCAGCCC 
TCGACATCCA CTGGAATCCC TAAAGTTCTG CCTCCCCCAG AACCGCAAAA ACCAGAATCT 
CCAATTGTTG GCAAGATCAT TAGGAAGGAA CAGCACAAAG TGCCTCGTAC CATGGAAGAC 
GGCATCGATA TGAGCTACCA GTACTTCGTG TCTATTCCCC TAAAGCGTGG CAGGAAGCCA 
CAGGTAGTTC GCTACCTTTA CCGCCCAATG GTGCGTCAAC TTAACGCTCC CACTTCGCCC 
AATCGACGCT CGAGGCGGGC AAGTAGAAAA GCAGCAGCGG CGGCAGCAGC TGCAGCGGCC 
AGCGAAGAAG AGCAGTTAAA GGCAGCGGAG GAGGGATCAC CTAAGATAGA TCCCGAGCTG 
AAAGCACTGG GCGGAGTGCC TGTGCCGCTG GAGGATCCAC CGCAGGTTAA GGAGCCATCA 
CCACAACCGG CAGTTAAGCC AAAGATATTC GTAGATCCCG AGGCAGTTCT AAATGGTCCG 
TACGAGGTGC CACCGCTCAA GCTGGATGCC CGCTACAAGT CGATGATGGA GCAGTTGGCC 
CAGATGCCAT ATCCCGAGGA TCGATCATCT CGTGCTCGTC GTCGACGCTC CAATCGCCAA 
GCTCGCGCTG CCGGTGGCGC TGACCAGGTG CCTACATCGG TGCATAGCCT GGGCGTTGGA 
GACGGTGACG CGCCTAAAAG TGATATGAAC TCCCTGCAGC AGGTGTCCTC CATATGGAAA 
TCTGGTTCGC GTTTGCCAAA ACCAGTCAAC TATCAACCAG AACCTAAACG TCTGTGCACG 
GCTACCGGTG CTCCCATGTC ATTTCATTCT CCGGTGCAAT TCGGACACTC CGTGGCGGAC 
TCGGACCACT TTATTCTGCC AGATGAGCCA ATGATTCAGG CCATAACCTA TGACGACATC 
GAACAGACGG AGCAAAAGCT ACTGGATGAG AAGCCGGTTC AAAAGGAGGA GGAACGCAAA 
GAGGGAACAA CCAAATCAGT GAGCTTTCAT CCTGTCGAGG CAGAAGTAAG TGAGTTCCAC 
TTGAGCAGCA GCTCCATCAG CATCAGCTTA ACTTCGAGCA GCAACTGTGG CGTAACCAAG 
ACCAAAACCT CTCGCCCCTC CAAGAAGAGG AAGTCCAAGT CCAAGGGAAG GGGCAAGTTG 
CGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727413.1
CDS	144..3392
Translation	MSTEMSKFLGGGRRYPVNRNKALQTKTGTGVAAKGGEPTARGAPSKPPSQRNPKPTKDADVPGAAGASRLGGGTSASQPPVKSNPRPSNGASGPSAAGAVPKRPPNRASPATAVTAEAGKAVPKRATGGGVPARGVMQTTATSTLRRRPAPPTSARIPISRRLDEAKWIMKPSDNFAPCSESSSEDNFKEQASGSQERKEDHAGDLDGSSSSGHLHRSQLLMSLPAEQVGRTKIKGRTSRIFRVPSQRIMHVDQLMQAQLRQMDSRKTRAQSSSRSHSRSPNWSASRLPSPLSQQSSFRSPSISTSSNEIAEVKDVALRDARTVTKIPATGLESCSPSPDASPATSQSRLQSPTISNASSCCTMENGVRSSMSTSSPHSNTTSSKTSVIECINGIRTSSTVQQNPGIQGALGSKSIKLTSTVMRLIQPVASKGDVESPEIPMEKDNEKEKEKKKLTVQKGNAKTKLAMNAPMVQSISPPKFSPMVKVPIPISQSVNHVVNQLTAQLKEAAMMHKSKDNLPKDRDTYEAFGPKDPFRKASIYLIMDQPRNQSGASTNEPTKDSWVIQPIEQPNSSTINLHKNEVTNPYLDESIEHPRQYINQRTVRAASQQRNESKSPSVNHMISLPRDSTGVNHSMNQYVMPPKTRRHPMRNPSKSSLLPRSRESKAGISRNPYIEESKTLSRFPARRPPSVSVNRPRQPSTSTGIPKVLPPPEPQKPESPIVGKIIRKEQHKVPRTMEDGIDMSYQYFVSIPLKRGRKPQVVRYLYRPMVRQLNAPTSPNRRSRRASRKAAAAAAAAAASEEEQLKAAEEGSPKIDPELKALGGVPVPLEDPPQVKEPSPQPAVKPKIFVDPEAVLNGPYEVPPLKLDARYKSMMEQLAQMPYPEDRSSRARRRRSNRQARAAGGADQVPTSVHSLGVGDGDAPKSDMNSLQQVSSIWKSGSRLPKPVNYQPEPKRLCTATGAPMSFHSPVQFGHSVADSDHFILPDEPMIQAITYDDIEQTEQKLLDEKPVQKEEERKEGTTKSVSFHPVEAEVSEFHLSSSSISISLTSSSNCGVTKTKTSRPSKKRKSKSKGRGKLRK

Target ORF information:

RefSeq Version	NM_167226.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32686), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167226.2

ORF Insert Sequence:

ATGTCTACGG AAATGTCAAA ATTCTTGGGC GGAGGCCGCC GTTATCCAGT AAATCGAAAC 
AAGGCGCTCC AGACCAAAAC AGGAACTGGT GTCGCCGCAA AAGGTGGCGA GCCAACAGCA 
CGTGGTGCTC CATCGAAACC ACCAAGCCAA AGAAATCCAA AGCCCACAAA GGACGCAGAT 
GTACCAGGTG CAGCAGGAGC TTCAAGACTA GGAGGAGGAA CGAGTGCATC ACAGCCGCCA 
GTCAAATCGA ATCCAAGACC ATCGAATGGT GCTAGTGGAC CATCAGCAGC TGGAGCTGTG 
CCCAAGAGAC CACCGAATCG AGCAAGTCCT GCAACTGCGG TGACAGCAGA AGCTGGCAAG 
GCAGTGCCCA AGAGAGCAAC TGGTGGTGGT GTTCCTGCAA GGGGTGTTAT GCAAACCACT 
GCCACATCCA CGTTGCGTCG AAGGCCAGCT CCACCGACTT CTGCAAGGAT ACCCATATCG 
CGGCGCCTAG ACGAGGCTAA GTGGATCATG AAGCCATCGG ATAACTTTGC GCCGTGCAGC 
GAGTCCTCCT CCGAGGATAA TTTCAAGGAA CAGGCCAGCG GATCGCAGGA GCGCAAAGAG 
GATCACGCTG GTGATTTGGA CGGGAGCAGT AGCAGTGGCC ATCTGCACAG GTCGCAGCTA 
CTGATGTCTT TGCCGGCTGA ACAGGTTGGG CGGACAAAGA TCAAGGGACG AACATCGCGC 
ATATTCCGTG TGCCCAGCCA GAGGATCATG CACGTCGATC AGCTGATGCA GGCACAGCTG 
CGCCAGATGG ATAGCCGAAA AACTAGAGCC CAGTCCAGCA GTCGTTCACA TAGTAGGTCC 
CCCAACTGGT CAGCCAGTAG GTTACCAAGT CCATTGTCCC AACAATCATC CTTTAGATCG 
CCAAGCATCT CGACCAGTTC AAACGAAATT GCGGAGGTAA AGGATGTGGC GCTGCGTGAT 
GCCAGAACCG TAACCAAGAT ACCCGCCACC GGGCTGGAAT CCTGCTCTCC CAGTCCGGAC 
GCTAGTCCCG CTACCAGCCA GAGTCGCCTT CAGAGCCCAA CCATTAGCAA CGCTTCCAGC 
TGCTGTACCA TGGAGAACGG TGTAAGGAGT AGCATGTCCA CCTCAAGTCC CCACTCGAAC 
ACGACCAGCA GCAAGACCAG TGTGATAGAG TGCATCAATG GGATCAGGAC GTCCAGCACC 
GTGCAACAGA ATCCTGGAAT TCAAGGGGCA TTGGGTAGCA AGTCCATTAA GCTAACCTCA 
ACGGTGATGA GGCTCATCCA GCCTGTCGCA TCGAAAGGTG ACGTCGAATC GCCGGAAATT 
CCCATGGAGA AGGATAACGA GAAGGAGAAG GAGAAGAAAA AGCTGACCGT GCAGAAAGGA 
AACGCCAAAA CTAAGCTGGC CATGAACGCA CCCATGGTCC AATCGATTTC GCCACCAAAG 
TTCAGTCCCA TGGTTAAGGT ACCGATACCA ATCAGTCAGT CGGTAAATCA TGTCGTCAAT 
CAGCTAACCG CTCAACTGAA GGAAGCTGCC ATGATGCACA AATCGAAAGA CAATCTACCG 
AAGGATCGTG ATACGTATGA AGCATTCGGA CCAAAGGATC CCTTTAGAAA AGCTTCCATT 
TATCTAATCA TGGATCAGCC AAGAAATCAA TCAGGTGCTT CGACAAACGA ACCTACAAAG 
GATTCGTGGG TTATTCAACC AATAGAGCAA CCAAACTCAT CTACGATTAA TCTGCACAAG 
AATGAAGTGA CGAATCCTTA TTTAGACGAA TCAATTGAAC ATCCCAGGCA ATATATAAAC 
CAAAGAACGG TGAGAGCGGC AAGTCAGCAG AGAAATGAAT CAAAGAGCCC ATCTGTGAAT 
CATATGATAA GCCTTCCAAG GGACTCAACT GGAGTTAATC ATTCAATGAA TCAGTATGTA 
ATGCCGCCAA AGACTAGAAG GCACCCAATG AGGAATCCAT CGAAAAGCTC TTTGCTTCCG 
AGGTCCAGAG AGTCGAAGGC AGGAATATCA AGGAATCCGT ACATTGAAGA GAGCAAAACT 
TTATCGAGAT TTCCAGCCCG GAGACCACCT TCCGTTTCGG TCAATAGACC GCGCCAGCCC 
TCGACATCCA CTGGAATCCC TAAAGTTCTG CCTCCCCCAG AACCGCAAAA ACCAGAATCT 
CCAATTGTTG GCAAGATCAT TAGGAAGGAA CAGCACAAAG TGCCTCGTAC CATGGAAGAC 
GGCATCGATA TGAGCTACCA GTACTTCGTG TCTATTCCCC TAAAGCGTGG CAGGAAGCCA 
CAGGTAGTTC GCTACCTTTA CCGCCCAATG GTGCGTCAAC TTAACGCTCC CACTTCGCCC 
AATCGACGCT CGAGGCGGGC AAGTAGAAAA GCAGCAGCGG CGGCAGCAGC TGCAGCGGCC 
AGCGAAGAAG AGCAGTTAAA GGCAGCGGAG GAGGGATCAC CTAAGATAGA TCCCGAGCTG 
AAAGCACTGG GCGGAGTGCC TGTGCCGCTG GAGGATCCAC CGCAGGTTAA GGAGCCATCA 
CCACAACCGG CAGTTAAGCC AAAGATATTC GTAGATCCCG AGGCAGTTCT AAATGGTCCG 
TACGAGGTGC CACCGCTCAA GCTGGATGCC CGCTACAAGT CGATGATGGA GCAGTTGGCC 
CAGATGCCAT ATCCCGAGGA TCGATCATCT CGTGCTCGTC GTCGACGCTC CAATCGCCAA 
GCTCGCGCTG CCGGTGGCGC TGACCAGGTG CCTACATCGG TGCATAGCCT GGGCGTTGGA 
GACGGTGACG CGCCTAAAAG TGATATGAAC TCCCTGCAGC AGGTGTCCTC CATATGGAAA 
TCTGGTTCGC GTTTGCCAAA ACCAGTCAAC TATCAACCAG AACCTAAACG TCTGTGCACG 
GCTACCGGTG CTCCCATGTC ATTTCATTCT CCGGTGCAAT TCGGACACTC CGTGGCGGAC 
TCGGACCACT TTATTCTGCC AGATGAGCCA ATGATTCAGG CCATAACCTA TGACGACATC 
GAACAGACGG AGCAAAAGCT ACTGGATGAG AAGCCGGTTC AAAAGGAGGA GGAACGCAAA 
GAGGGAACAA CCAAATCAGT GAGCTTTCAT CCTGTCGAGG CAGAAGTAAG TGAGTTCCAC 
TTGAGCAGCA GCTCCATCAG CATCAGCTTA ACTTCGAGCA GCAACTGTGG CGTAACCAAG 
ACCAAAACCT CTCGCCCCTC CAAGAAGAGG AAGTCCAAGT CCAAGGGAAG GGGCAAGTTG 
CGTAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32686 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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