l(1)G0020 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	l(1)G0020
Entrez Gene ID	31811
Full Name	lethal (1) G0020
Synonyms	CG1994,Dmel\CG1994,NAT10,p110
General protein information	Preferred Names lethal (1) G0020 Names CG1994 gene product from transcript CG1994-RA CG1994-PA CG1994-PB l(1)G0020-PA l(1)G0020-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8A2-8A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_132275.2	NP_572503.1	lethal (1) G0020, isoform A
NM_001298096.1	NP_001285025.1	lethal (1) G0020, isoform B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nat10	NP_001101226.1
Saccharomyces cerevisiae (baker's yeast)	KRE33	NP_014267.1
Arabidopsis thaliana (thale cress)	AT3G57940	NP_191353.2
Canis lupus familiaris (dog)	NAT10	XP_005631180.1
Bos taurus (cattle)	NAT10	NP_001091500.1
Oryza sativa (rice)	Os12g0170700	NP_001066273.1
Xenopus tropicalis (tropical clawed frog)	nat10	NP_001184159.1
Pan troglodytes (chimpanzee)	NAT10	XP_001146522.2
Gallus gallus (chicken)	NAT10	NP_001012958.1
Drosophila melanogaster (fruit fly)	l(1)G0020	NP_572503.1
Kluyveromyces lactis	KLLA0F24398g	XP_456167.1
Homo sapiens (human)	NAT10	NP_078938.2
Mus musculus (house mouse)	Nat10	NP_694766.1
Danio rerio (zebrafish)	nat10	NP_956938.1
Caenorhabditis elegans (roundworm)	nath-10	NP_491574.1
Magnaporthe oryzae (rice blast fungus)	MGG_11039	XP_003712899.1
	NAT10	XP_001115721.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010491	XP_314465.4
Eremothecium gossypii	AGOS_AGR346C	NP_987012.2
Schizosaccharomyces pombe (fission yeast)	SPAC20G8.09c	NP_593326.1
Neurospora crassa	NCU10654	XP_001728354.1
Arabidopsis thaliana (thale cress)	AT1G10490	NP_172519.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following l(1)G0020 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(1)G0020 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa21400	NM_001298096.1 Latest version!	Drosophila melanogaster lethal (1) G0020 (l(1)G0020), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03290	NM_132275.2 Latest version!	Drosophila melanogaster lethal (1) G0020 (l(1)G0020), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

l(1)G0020 ( NM_001298096.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285025.1 Drosophila melanogaster lethal (1) G0020 (l(1)G0020), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21400
Clone ID Related Accession (Same CDS sequence)	NM_001298096.1
Accession Version	NM_001298096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (1) G0020, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAGA AGAAAATAGA CAACCGGATA CGCGTGATGA TCGAGAATGG GGTCAAGCTG 
GGCCACCGAA CCATGTTCAT CGTCATCGGG GACAAGGCGC GCGACCAGGT TCCCATTCTT 
TACGACATCC TGACCAAGTC GACGGTCAAG GCGCGACCCA CGGTCCTGTG GTGCTACAAG 
AACAAGGATG AGGCCATCTC CAATCATGGC AAAAAGCGAG CAAAGAAGAT TGCTGTGGGC 
AAAGTGGATG TCAATGAGGC GGACCTGTTC GATTCCTTTC GAGTGGCCAC CACCATTCAC 
GGCAGATACT ACTCCGAGAC GCACGCAGTC CTCGGACGAA CCTACGGCGT GTGTGTGCTC 
CAGGACTTCG AGGCCCTGAC CCCCAATCTT TTGGCTCGAA CTGTGGAAAC GGTTGAGGGC 
GGCGGTCTGA TCATCCTGCT GCTGAAGACA CTGCAGTCGC TGAAACAGTT GTACACCATG 
AGCATGGATG TGCACAAGCG ATTCCGCACA GAAGCCCATC AGACGGTGAC CTGTCGATTC 
AATGAGCGAC TCATTCTCTC CCTGGCGGAC TGCAAGCGCT GTCTGGTGGT CAACGATGAC 
CTCACCGTGC TGCCACTTAG CTCCAAGACC ATCAACGTGG AGCCAGTGAA TCCCGCAGGC 
GCCGGTCGAT CGCCCAACGA GGCTAGCCTC AAGGAGCTGA AGGAGAGCCT GCTCACAGTT 
CAACCAGCAG GAGCTCTGGT TAATCTCTGC AAGACCTACG ATCAGGCCAA TGCCGTTGCC 
CAGTTCATTG AGGCGCTGGT CGACAAGCAA CTGAAGCCGC CCATGTCCTT AACAGCGGCT 
CGTGGTCGTG GTAAATCGGC CGCCCTGGGT CTGTCGATTG CCGCCGCCGT CGCCTTTGGC 
TATGTGAACA TCTACGTTAC CTCACCGCAT CCGGAGAACT TAATCACGCT CTTTGAATTT 
GTGCTCAAGG GCTTCGATGC CTTGGAGTAC CAGGAGCATG CTGACTACAC CATTATACGC 
TCCACCAATG CCGACTACAA AAAGGCGATA ATAAGAATAA ATATTACGAG AAGCAGCAGG 
CAGACCATTC AGTACATAGC TCCCAGCGAT ACTCATCTGC TGAACGCAGC CGATCTTCTG 
CTGATCGATG AGGCGGCTGC CATTCCTTTG CCGCTGGTAA AGAAGATGAT CGGTCCGTAT 
TTGGTATTCA TGGCCAGTAC GATAAATGGA TACGAGGGCA CTGGCAGATC GCTGAGCTTG 
AAGCTCATTT CCCAGCTGCA AAAGGATAAC AATGCAAGGC CGCCACTGAA ACTGGAGGAA 
TCGATTCGGT ATCAGGAGAA CGACGACATA GAAAAGTGGC TGATCAACCT GCTGTGCTTG 
GATGCCAGTA CGGTGCCGAG TATTAGCTCG GGCTGTCCCA CGCCGGATGC TTGTGAACTG 
TACTATGTGG ACAGGGACGC CCTGTTTTCG TATCACAAGG CTGCCGAGGC CTTTCTCCAT 
CGCCTGGTCT CCATTTATGT GTCCTCGCAC TACAAGAACA CACCAAACGA TTTGCAGATG 
ATGAGCGATG CCCCGGCGCA TCATCTATTC TGTCTACTGG GTCCGGTTCA GCGAATGGAC 
GCCCTGCCAG AGATATTGGT GGTTATCCAA GTGGCACTGG AGGGTCAGAT CTCCGCCCAG 
AGCATCAGCG ATTCCCTGGG CAGGGGCAAG AAGGCGGCGG GCGATCTGAT TCCCTGGAAC 
GTAGCCGAGC AGTATGGCGA TCGAGATTTT CCCAAGCTGT GTGGCGTTCG CATTGTGAGG 
GTAGCCACGC ATCCGAATTA CCAGAGGATG GGCTATGGCA AGCGGGCCAT TCAGCTGCTC 
AAGGACTACT ATGCAAGGAA GCACACTAAT CTGGAGGACG GTCCAGTTGC CAGCAAGGAT 
GCTGGTAAAG GTATCGAGGA AGTGGAGGAG GAGGAACTTA GTCTGCTGAA GGAACAAATC 
CGGCCACGTA GTCGTATACC CACACTTCTG CAGCGCTTGC ACGAACGTGT CCCGGAACAC 
GTGGACTATA TTGGCACATC CTATGGCCTC ACCACCGAGC TGCTGAAGTT CTGGAAGAAC 
GCCGGCTTTG TGCCCGTCTA TTTGAGCCAG AAATCTAACG AACTGACCGC CGAGCATAGC 
TGCATTATGC TGCACACGCC CAATGCCACG CCCTGGCTGG GCCTGTACTA CCAGGACTTC 
CGCAGGAGAG TCCTCAAGTT GATGGGAAAG ACGTTCAGGG AATTCGAGAC GAAACTCTGT 
TTGGCCTTGC TGAAGAACAA GTCCGTGGAC ACGGAAGGTA GTGCACTGAA AGTACTCGAT 
AAGCCAATGC TGGATGTTTA CTTCTTGCCG CACGATCTGC AGCGCTTGGA GAGCTACGCC 
CGCCAGCAGT CCGAGTTTCG TCTAATCATC GATCTGCTCA CGGACATTGC CCAGCTGTAT 
TTTCAGGGCA GGATCGATGG TCTGCAGCTT GACCTGGTGC AGCAGGGCAT CCTTTTGGCT 
CTGGGCGTGC AGGGCAAGAC TGTGGATGCG TTGGGTCTGG AGCTGAATAT GCCGGGTAAC 
CAGTTGTTGG CGAAATTTTT CGATGCCATG AAACGGTGCA ATCAGTGCTT CCGGTCGGTA 
CTGGAGGAGC ATATCGAAGG CGGCATGCTG CGGGAGGCGG ATCTGTCCAA GGGCGAGGAA 
CTGCAACCGC TGACTCTGTC CCTCGACAAG GAGCTGGATC AAACCGCTCA GAAGCTGAGC 
AAGCAGCAGC GCAAGGAGCT GAAGCGCCTG AAGGCCGAGC AGCTGGACGA ATTCCAAATC 
AAGGGCACCG AAGAGGATTG GTCCAAGGCG CTGGAAACAA ACGGAACAGG CGGTGGCAGC 
GGGCTGCTCT CCGTCAAGAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285025.1
CDS	113..3016
Translation	MVKKKIDNRIRVMIENGVKLGHRTMFIVIGDKARDQVPILYDILTKSTVKARPTVLWCYKNKDEAISNHGKKRAKKIAVGKVDVNEADLFDSFRVATTIHGRYYSETHAVLGRTYGVCVLQDFEALTPNLLARTVETVEGGGLIILLLKTLQSLKQLYTMSMDVHKRFRTEAHQTVTCRFNERLILSLADCKRCLVVNDDLTVLPLSSKTINVEPVNPAGAGRSPNEASLKELKESLLTVQPAGALVNLCKTYDQANAVAQFIEALVDKQLKPPMSLTAARGRGKSAALGLSIAAAVAFGYVNIYVTSPHPENLITLFEFVLKGFDALEYQEHADYTIIRSTNADYKKAIIRINITRSSRQTIQYIAPSDTHLLNAADLLLIDEAAAIPLPLVKKMIGPYLVFMASTINGYEGTGRSLSLKLISQLQKDNNARPPLKLEESIRYQENDDIEKWLINLLCLDASTVPSISSGCPTPDACELYYVDRDALFSYHKAAEAFLHRLVSIYVSSHYKNTPNDLQMMSDAPAHHLFCLLGPVQRMDALPEILVVIQVALEGQISAQSISDSLGRGKKAAGDLIPWNVAEQYGDRDFPKLCGVRIVRVATHPNYQRMGYGKRAIQLLKDYYARKHTNLEDGPVASKDAGKGIEEVEEEELSLLKEQIRPRSRIPTLLQRLHERVPEHVDYIGTSYGLTTELLKFWKNAGFVPVYLSQKSNELTAEHSCIMLHTPNATPWLGLYYQDFRRRVLKLMGKTFREFETKLCLALLKNKSVDTEGSALKVLDKPMLDVYFLPHDLQRLESYARQQSEFRLIIDLLTDIAQLYFQGRIDGLQLDLVQQGILLALGVQGKTVDALGLELNMPGNQLLAKFFDAMKRCNQCFRSVLEEHIEGGMLREADLSKGEELQPLTLSLDKELDQTAQKLSKQQRKELKRLKAEQLDEFQIKGTEEDWSKALETNGTGGGSGLLSVKR

Target ORF information:

RefSeq Version	NM_001298096.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (1) G0020 (l(1)G0020), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298096.1

ORF Insert Sequence:

ATGGTGAAGA AGAAAATAGA CAACCGGATA CGCGTGATGA TCGAGAATGG GGTCAAGCTG 
GGCCACCGAA CCATGTTCAT CGTCATCGGG GACAAGGCGC GCGACCAGGT TCCCATTCTT 
TACGACATCC TGACCAAGTC GACGGTCAAG GCGCGACCCA CGGTCCTGTG GTGCTACAAG 
AACAAGGATG AGGCCATCTC CAATCATGGC AAAAAGCGAG CAAAGAAGAT TGCTGTGGGC 
AAAGTGGATG TCAATGAGGC GGACCTGTTC GATTCCTTTC GAGTGGCCAC CACCATTCAC 
GGCAGATACT ACTCCGAGAC GCACGCAGTC CTCGGACGAA CCTACGGCGT GTGTGTGCTC 
CAGGACTTCG AGGCCCTGAC CCCCAATCTT TTGGCTCGAA CTGTGGAAAC GGTTGAGGGC 
GGCGGTCTGA TCATCCTGCT GCTGAAGACA CTGCAGTCGC TGAAACAGTT GTACACCATG 
AGCATGGATG TGCACAAGCG ATTCCGCACA GAAGCCCATC AGACGGTGAC CTGTCGATTC 
AATGAGCGAC TCATTCTCTC CCTGGCGGAC TGCAAGCGCT GTCTGGTGGT CAACGATGAC 
CTCACCGTGC TGCCACTTAG CTCCAAGACC ATCAACGTGG AGCCAGTGAA TCCCGCAGGC 
GCCGGTCGAT CGCCCAACGA GGCTAGCCTC AAGGAGCTGA AGGAGAGCCT GCTCACAGTT 
CAACCAGCAG GAGCTCTGGT TAATCTCTGC AAGACCTACG ATCAGGCCAA TGCCGTTGCC 
CAGTTCATTG AGGCGCTGGT CGACAAGCAA CTGAAGCCGC CCATGTCCTT AACAGCGGCT 
CGTGGTCGTG GTAAATCGGC CGCCCTGGGT CTGTCGATTG CCGCCGCCGT CGCCTTTGGC 
TATGTGAACA TCTACGTTAC CTCACCGCAT CCGGAGAACT TAATCACGCT CTTTGAATTT 
GTGCTCAAGG GCTTCGATGC CTTGGAGTAC CAGGAGCATG CTGACTACAC CATTATACGC 
TCCACCAATG CCGACTACAA AAAGGCGATA ATAAGAATAA ATATTACGAG AAGCAGCAGG 
CAGACCATTC AGTACATAGC TCCCAGCGAT ACTCATCTGC TGAACGCAGC CGATCTTCTG 
CTGATCGATG AGGCGGCTGC CATTCCTTTG CCGCTGGTAA AGAAGATGAT CGGTCCGTAT 
TTGGTATTCA TGGCCAGTAC GATAAATGGA TACGAGGGCA CTGGCAGATC GCTGAGCTTG 
AAGCTCATTT CCCAGCTGCA AAAGGATAAC AATGCAAGGC CGCCACTGAA ACTGGAGGAA 
TCGATTCGGT ATCAGGAGAA CGACGACATA GAAAAGTGGC TGATCAACCT GCTGTGCTTG 
GATGCCAGTA CGGTGCCGAG TATTAGCTCG GGCTGTCCCA CGCCGGATGC TTGTGAACTG 
TACTATGTGG ACAGGGACGC CCTGTTTTCG TATCACAAGG CTGCCGAGGC CTTTCTCCAT 
CGCCTGGTCT CCATTTATGT GTCCTCGCAC TACAAGAACA CACCAAACGA TTTGCAGATG 
ATGAGCGATG CCCCGGCGCA TCATCTATTC TGTCTACTGG GTCCGGTTCA GCGAATGGAC 
GCCCTGCCAG AGATATTGGT GGTTATCCAA GTGGCACTGG AGGGTCAGAT CTCCGCCCAG 
AGCATCAGCG ATTCCCTGGG CAGGGGCAAG AAGGCGGCGG GCGATCTGAT TCCCTGGAAC 
GTAGCCGAGC AGTATGGCGA TCGAGATTTT CCCAAGCTGT GTGGCGTTCG CATTGTGAGG 
GTAGCCACGC ATCCGAATTA CCAGAGGATG GGCTATGGCA AGCGGGCCAT TCAGCTGCTC 
AAGGACTACT ATGCAAGGAA GCACACTAAT CTGGAGGACG GTCCAGTTGC CAGCAAGGAT 
GCTGGTAAAG GTATCGAGGA AGTGGAGGAG GAGGAACTTA GTCTGCTGAA GGAACAAATC 
CGGCCACGTA GTCGTATACC CACACTTCTG CAGCGCTTGC ACGAACGTGT CCCGGAACAC 
GTGGACTATA TTGGCACATC CTATGGCCTC ACCACCGAGC TGCTGAAGTT CTGGAAGAAC 
GCCGGCTTTG TGCCCGTCTA TTTGAGCCAG AAATCTAACG AACTGACCGC CGAGCATAGC 
TGCATTATGC TGCACACGCC CAATGCCACG CCCTGGCTGG GCCTGTACTA CCAGGACTTC 
CGCAGGAGAG TCCTCAAGTT GATGGGAAAG ACGTTCAGGG AATTCGAGAC GAAACTCTGT 
TTGGCCTTGC TGAAGAACAA GTCCGTGGAC ACGGAAGGTA GTGCACTGAA AGTACTCGAT 
AAGCCAATGC TGGATGTTTA CTTCTTGCCG CACGATCTGC AGCGCTTGGA GAGCTACGCC 
CGCCAGCAGT CCGAGTTTCG TCTAATCATC GATCTGCTCA CGGACATTGC CCAGCTGTAT 
TTTCAGGGCA GGATCGATGG TCTGCAGCTT GACCTGGTGC AGCAGGGCAT CCTTTTGGCT 
CTGGGCGTGC AGGGCAAGAC TGTGGATGCG TTGGGTCTGG AGCTGAATAT GCCGGGTAAC 
CAGTTGTTGG CGAAATTTTT CGATGCCATG AAACGGTGCA ATCAGTGCTT CCGGTCGGTA 
CTGGAGGAGC ATATCGAAGG CGGCATGCTG CGGGAGGCGG ATCTGTCCAA GGGCGAGGAA 
CTGCAACCGC TGACTCTGTC CCTCGACAAG GAGCTGGATC AAACCGCTCA GAAGCTGAGC 
AAGCAGCAGC GCAAGGAGCT GAAGCGCCTG AAGGCCGAGC AGCTGGACGA ATTCCAAATC 
AAGGGCACCG AAGAGGATTG GTCCAAGGCG CTGGAAACAA ACGGAACAGG CGGTGGCAGC 
GGGCTGCTCT CCGTCAAGAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

l(1)G0020 ( NM_132275.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572503.1 Drosophila melanogaster lethal (1) G0020 (l(1)G0020), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03290
Clone ID Related Accession (Same CDS sequence)	NM_132275.2
Accession Version	NM_132275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lethal (1) G0020, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132275.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGAAGA AGAAAATAGA CAACCGGATA CGCGTGATGA TCGAGAATGG GGTCAAGCTG 
GGCCACCGAA CCATGTTCAT CGTCATCGGG GACAAGGCGC GCGACCAGGT TCCCATTCTT 
TACGACATCC TGACCAAGTC GACGGTCAAG GCGCGACCCA CGGTCCTGTG GTGCTACAAG 
AACAAGGATG AGGCCATCTC CAATCATGGC AAAAAGCGAG CAAAGAAGAT TGCTGTGGGC 
AAAGTGGATG TCAATGAGGC GGACCTGTTC GATTCCTTTC GAGTGGCCAC CACCATTCAC 
GGCAGATACT ACTCCGAGAC GCACGCAGTC CTCGGACGAA CCTACGGCGT GTGTGTGCTC 
CAGGACTTCG AGGCCCTGAC CCCCAATCTT TTGGCTCGAA CTGTGGAAAC GGTTGAGGGC 
GGCGGTCTGA TCATCCTGCT GCTGAAGACA CTGCAGTCGC TGAAACAGTT GTACACCATG 
AGCATGGATG TGCACAAGCG ATTCCGCACA GAAGCCCATC AGACGGTGAC CTGTCGATTC 
AATGAGCGAC TCATTCTCTC CCTGGCGGAC TGCAAGCGCT GTCTGGTGGT CAACGATGAC 
CTCACCGTGC TGCCACTTAG CTCCAAGACC ATCAACGTGG AGCCAGTGAA TCCCGCAGGC 
GCCGGTCGAT CGCCCAACGA GGCTAGCCTC AAGGAGCTGA AGGAGAGCCT GCTCACAGTT 
CAACCAGCAG GAGCTCTGGT TAATCTCTGC AAGACCTACG ATCAGGCCAA TGCCGTTGCC 
CAGTTCATTG AGGCGCTGGT CGACAAGCAA CTGAAGCCGC CCATGTCCTT AACAGCGGCT 
CGTGGTCGTG GTAAATCGGC CGCCCTGGGT CTGTCGATTG CCGCCGCCGT CGCCTTTGGC 
TATGTGAACA TCTACGTTAC CTCACCGCAT CCGGAGAACT TAATCACGCT CTTTGAATTT 
GTGCTCAAGG GCTTCGATGC CTTGGAGTAC CAGGAGCATG CTGACTACAC CATTATACGC 
TCCACCAATG CCGACTACAA AAAGGCGATA ATAAGAATAA ATATTACGAG AAGCAGCAGG 
CAGACCATTC AGTACATAGC TCCCAGCGAT ACTCATCTGC TGAACGCAGC CGATCTTCTG 
CTGATCGATG AGGCGGCTGC CATTCCTTTG CCGCTGGTAA AGAAGATGAT CGGTCCGTAT 
TTGGTATTCA TGGCCAGTAC GATAAATGGA TACGAGGGCA CTGGCAGATC GCTGAGCTTG 
AAGCTCATTT CCCAGCTGCA AAAGGATAAC AATGCAAGGC CGCCACTGAA ACTGGAGGAA 
TCGATTCGGT ATCAGGAGAA CGACGACATA GAAAAGTGGC TGATCAACCT GCTGTGCTTG 
GATGCCAGTA CGGTGCCGAG TATTAGCTCG GGCTGTCCCA CGCCGGATGC TTGTGAACTG 
TACTATGTGG ACAGGGACGC CCTGTTTTCG TATCACAAGG CTGCCGAGGC CTTTCTCCAT 
CGCCTGGTCT CCATTTATGT GTCCTCGCAC TACAAGAACA CACCAAACGA TTTGCAGATG 
ATGAGCGATG CCCCGGCGCA TCATCTATTC TGTCTACTGG GTCCGGTTCA GCGAATGGAC 
GCCCTGCCAG AGATATTGGT GGTTATCCAA GTGGCACTGG AGGGTCAGAT CTCCGCCCAG 
AGCATCAGCG ATTCCCTGGG CAGGGGCAAG AAGGCGGCGG GCGATCTGAT TCCCTGGAAC 
GTAGCCGAGC AGTATGGCGA TCGAGATTTT CCCAAGCTGT GTGGCGTTCG CATTGTGAGG 
GTAGCCACGC ATCCGAATTA CCAGAGGATG GGCTATGGCA AGCGGGCCAT TCAGCTGCTC 
AAGGACTACT ATGCAAGGAA GCACACTAAT CTGGAGGACG GTCCAGTTGC CAGCAAGGAT 
GCTGGTAAAG GTATCGAGGA AGTGGAGGAG GAGGAACTTA GTCTGCTGAA GGAACAAATC 
CGGCCACGTA GTCGTATACC CACACTTCTG CAGCGCTTGC ACGAACGTGT CCCGGAACAC 
GTGGACTATA TTGGCACATC CTATGGCCTC ACCACCGAGC TGCTGAAGTT CTGGAAGAAC 
GCCGGCTTTG TGCCCGTCTA TTTGAGCCAG AAATCTAACG AACTGACCGC CGAGCATAGC 
TGCATTATGC TGCACACGCC CAATGCCACG CCCTGGCTGG GCCTGTACTA CCAGGACTTC 
CGCAGGAGAG TCCTCAAGTT GATGGGAAAG ACGTTCAGGG AATTCGAGAC GAAACTCTGT 
TTGGCCTTGC TGAAGAACAA GTCCGTGGAC ACGGAAGGTA GTGCACTGAA AGTACTCGAT 
AAGCCAATGC TGGATGTTTA CTTCTTGCCG CACGATCTGC AGCGCTTGGA GAGCTACGCC 
CGCCAGCAGT CCGAGTTTCG TCTAATCATC GATCTGCTCA CGGACATTGC CCAGCTGTAT 
TTTCAGGGCA GGATCGATGG TCTGCAGCTT GACCTGGTGC AGCAGGGCAT CCTTTTGGCT 
CTGGGCGTGC AGGGCAAGAC TGTGGATGCG TTGGGTCTGG AGCTGAATAT GCCGGGTAAC 
CAGTTGTTGG CGAAATTTTT CGATGCCATG AAACGGTGCA ATCAGTGCTT CCGGTCGGTA 
CTGGAGGAGC ATATCGAAGG CGGCATGCTG CGGGAGGCGG ATCTGTCCAA GGGCGAGGAA 
CTGCAACCGC TGACTCTGTC CCTCGACAAG GAGCTGGATC AAACCGCTCA GAAGCTGAGC 
AAGCAGCAGC GCAAGGAGCT GAAGCGCCTG AAGGCCGAGC AGCTGGACGA ATTCCAAATC 
AAGGGCACCG AAGAGGATTG GTCCAAGGCG CTGGAAACAA ACGGAACAGG CGGTGGCAGC 
GGGCTGCTCT CCGTCAAGAG TGGCGTAAAG CGACTGGATG GACCAATCGA GACGCGTGAG 
GATGGCGACC TGGCAGCTCC GCTGTCCAAG AAGAAAAAGA AGAACAATCC GAAACAGAGA 
CGCAGTCAGG GCAAGTCCCT GATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572503.1
CDS	113..3139
Translation	MVKKKIDNRIRVMIENGVKLGHRTMFIVIGDKARDQVPILYDILTKSTVKARPTVLWCYKNKDEAISNHGKKRAKKIAVGKVDVNEADLFDSFRVATTIHGRYYSETHAVLGRTYGVCVLQDFEALTPNLLARTVETVEGGGLIILLLKTLQSLKQLYTMSMDVHKRFRTEAHQTVTCRFNERLILSLADCKRCLVVNDDLTVLPLSSKTINVEPVNPAGAGRSPNEASLKELKESLLTVQPAGALVNLCKTYDQANAVAQFIEALVDKQLKPPMSLTAARGRGKSAALGLSIAAAVAFGYVNIYVTSPHPENLITLFEFVLKGFDALEYQEHADYTIIRSTNADYKKAIIRINITRSSRQTIQYIAPSDTHLLNAADLLLIDEAAAIPLPLVKKMIGPYLVFMASTINGYEGTGRSLSLKLISQLQKDNNARPPLKLEESIRYQENDDIEKWLINLLCLDASTVPSISSGCPTPDACELYYVDRDALFSYHKAAEAFLHRLVSIYVSSHYKNTPNDLQMMSDAPAHHLFCLLGPVQRMDALPEILVVIQVALEGQISAQSISDSLGRGKKAAGDLIPWNVAEQYGDRDFPKLCGVRIVRVATHPNYQRMGYGKRAIQLLKDYYARKHTNLEDGPVASKDAGKGIEEVEEEELSLLKEQIRPRSRIPTLLQRLHERVPEHVDYIGTSYGLTTELLKFWKNAGFVPVYLSQKSNELTAEHSCIMLHTPNATPWLGLYYQDFRRRVLKLMGKTFREFETKLCLALLKNKSVDTEGSALKVLDKPMLDVYFLPHDLQRLESYARQQSEFRLIIDLLTDIAQLYFQGRIDGLQLDLVQQGILLALGVQGKTVDALGLELNMPGNQLLAKFFDAMKRCNQCFRSVLEEHIEGGMLREADLSKGEELQPLTLSLDKELDQTAQKLSKQQRKELKRLKAEQLDEFQIKGTEEDWSKALETNGTGGGSGLLSVKSGVKRLDGPIETREDGDLAAPLSKKKKKNNPKQRRSQGKSLI

Target ORF information:

RefSeq Version	NM_132275.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lethal (1) G0020 (l(1)G0020), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132275.2

ORF Insert Sequence:

ATGGTGAAGA AGAAAATAGA CAACCGGATA CGCGTGATGA TCGAGAATGG GGTCAAGCTG 
GGCCACCGAA CCATGTTCAT CGTCATCGGG GACAAGGCGC GCGACCAGGT TCCCATTCTT 
TACGACATCC TGACCAAGTC GACGGTCAAG GCGCGACCCA CGGTCCTGTG GTGCTACAAG 
AACAAGGATG AGGCCATCTC CAATCATGGC AAAAAGCGAG CAAAGAAGAT TGCTGTGGGC 
AAAGTGGATG TCAATGAGGC GGACCTGTTC GATTCCTTTC GAGTGGCCAC CACCATTCAC 
GGCAGATACT ACTCCGAGAC GCACGCAGTC CTCGGACGAA CCTACGGCGT GTGTGTGCTC 
CAGGACTTCG AGGCCCTGAC CCCCAATCTT TTGGCTCGAA CTGTGGAAAC GGTTGAGGGC 
GGCGGTCTGA TCATCCTGCT GCTGAAGACA CTGCAGTCGC TGAAACAGTT GTACACCATG 
AGCATGGATG TGCACAAGCG ATTCCGCACA GAAGCCCATC AGACGGTGAC CTGTCGATTC 
AATGAGCGAC TCATTCTCTC CCTGGCGGAC TGCAAGCGCT GTCTGGTGGT CAACGATGAC 
CTCACCGTGC TGCCACTTAG CTCCAAGACC ATCAACGTGG AGCCAGTGAA TCCCGCAGGC 
GCCGGTCGAT CGCCCAACGA GGCTAGCCTC AAGGAGCTGA AGGAGAGCCT GCTCACAGTT 
CAACCAGCAG GAGCTCTGGT TAATCTCTGC AAGACCTACG ATCAGGCCAA TGCCGTTGCC 
CAGTTCATTG AGGCGCTGGT CGACAAGCAA CTGAAGCCGC CCATGTCCTT AACAGCGGCT 
CGTGGTCGTG GTAAATCGGC CGCCCTGGGT CTGTCGATTG CCGCCGCCGT CGCCTTTGGC 
TATGTGAACA TCTACGTTAC CTCACCGCAT CCGGAGAACT TAATCACGCT CTTTGAATTT 
GTGCTCAAGG GCTTCGATGC CTTGGAGTAC CAGGAGCATG CTGACTACAC CATTATACGC 
TCCACCAATG CCGACTACAA AAAGGCGATA ATAAGAATAA ATATTACGAG AAGCAGCAGG 
CAGACCATTC AGTACATAGC TCCCAGCGAT ACTCATCTGC TGAACGCAGC CGATCTTCTG 
CTGATCGATG AGGCGGCTGC CATTCCTTTG CCGCTGGTAA AGAAGATGAT CGGTCCGTAT 
TTGGTATTCA TGGCCAGTAC GATAAATGGA TACGAGGGCA CTGGCAGATC GCTGAGCTTG 
AAGCTCATTT CCCAGCTGCA AAAGGATAAC AATGCAAGGC CGCCACTGAA ACTGGAGGAA 
TCGATTCGGT ATCAGGAGAA CGACGACATA GAAAAGTGGC TGATCAACCT GCTGTGCTTG 
GATGCCAGTA CGGTGCCGAG TATTAGCTCG GGCTGTCCCA CGCCGGATGC TTGTGAACTG 
TACTATGTGG ACAGGGACGC CCTGTTTTCG TATCACAAGG CTGCCGAGGC CTTTCTCCAT 
CGCCTGGTCT CCATTTATGT GTCCTCGCAC TACAAGAACA CACCAAACGA TTTGCAGATG 
ATGAGCGATG CCCCGGCGCA TCATCTATTC TGTCTACTGG GTCCGGTTCA GCGAATGGAC 
GCCCTGCCAG AGATATTGGT GGTTATCCAA GTGGCACTGG AGGGTCAGAT CTCCGCCCAG 
AGCATCAGCG ATTCCCTGGG CAGGGGCAAG AAGGCGGCGG GCGATCTGAT TCCCTGGAAC 
GTAGCCGAGC AGTATGGCGA TCGAGATTTT CCCAAGCTGT GTGGCGTTCG CATTGTGAGG 
GTAGCCACGC ATCCGAATTA CCAGAGGATG GGCTATGGCA AGCGGGCCAT TCAGCTGCTC 
AAGGACTACT ATGCAAGGAA GCACACTAAT CTGGAGGACG GTCCAGTTGC CAGCAAGGAT 
GCTGGTAAAG GTATCGAGGA AGTGGAGGAG GAGGAACTTA GTCTGCTGAA GGAACAAATC 
CGGCCACGTA GTCGTATACC CACACTTCTG CAGCGCTTGC ACGAACGTGT CCCGGAACAC 
GTGGACTATA TTGGCACATC CTATGGCCTC ACCACCGAGC TGCTGAAGTT CTGGAAGAAC 
GCCGGCTTTG TGCCCGTCTA TTTGAGCCAG AAATCTAACG AACTGACCGC CGAGCATAGC 
TGCATTATGC TGCACACGCC CAATGCCACG CCCTGGCTGG GCCTGTACTA CCAGGACTTC 
CGCAGGAGAG TCCTCAAGTT GATGGGAAAG ACGTTCAGGG AATTCGAGAC GAAACTCTGT 
TTGGCCTTGC TGAAGAACAA GTCCGTGGAC ACGGAAGGTA GTGCACTGAA AGTACTCGAT 
AAGCCAATGC TGGATGTTTA CTTCTTGCCG CACGATCTGC AGCGCTTGGA GAGCTACGCC 
CGCCAGCAGT CCGAGTTTCG TCTAATCATC GATCTGCTCA CGGACATTGC CCAGCTGTAT 
TTTCAGGGCA GGATCGATGG TCTGCAGCTT GACCTGGTGC AGCAGGGCAT CCTTTTGGCT 
CTGGGCGTGC AGGGCAAGAC TGTGGATGCG TTGGGTCTGG AGCTGAATAT GCCGGGTAAC 
CAGTTGTTGG CGAAATTTTT CGATGCCATG AAACGGTGCA ATCAGTGCTT CCGGTCGGTA 
CTGGAGGAGC ATATCGAAGG CGGCATGCTG CGGGAGGCGG ATCTGTCCAA GGGCGAGGAA 
CTGCAACCGC TGACTCTGTC CCTCGACAAG GAGCTGGATC AAACCGCTCA GAAGCTGAGC 
AAGCAGCAGC GCAAGGAGCT GAAGCGCCTG AAGGCCGAGC AGCTGGACGA ATTCCAAATC 
AAGGGCACCG AAGAGGATTG GTCCAAGGCG CTGGAAACAA ACGGAACAGG CGGTGGCAGC 
GGGCTGCTCT CCGTCAAGAG TGGCGTAAAG CGACTGGATG GACCAATCGA GACGCGTGAG 
GATGGCGACC TGGCAGCTCC GCTGTCCAAG AAGAAAAAGA AGAACAATCC GAAACAGAGA 
CGCAGTCAGG GCAAGTCCCT GATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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l(1)G0020 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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