Ppt1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppt1
Entrez Gene ID	31805
Full Name	Palmitoyl-protein thioesterase 1
Synonyms	CG12108,DmPpt1,Dmel\CG12108,PPT1,ppt1
General protein information	Preferred Names Palmitoyl-protein thioesterase 1 Names CG12108 gene product from transcript CG12108-RB CG12108-PA CG12108-PB Ppt1-PA Ppt1-PB palmitoyl protein thioesterase 1 palmitoyl-protein thioesterase 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 8A2-8A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_167166.4	NP_727284.1	Palmitoyl-protein thioesterase 1, isoform A
NM_001298095.2	NP_001285024.1	Palmitoyl-protein thioesterase 1, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ppt1	NP_727284.1
Caenorhabditis elegans (roundworm)	ppt-1	NP_504684.2
Oryza sativa (rice)	Os03g0101100	NP_001048650.1
	PPT1	XP_001082462.1
Mus musculus (house mouse)	Ppt1	NP_032943.2
Schizosaccharomyces pombe (fission yeast)	pdf1	NP_595325.2
Magnaporthe oryzae (rice blast fungus)	MGG_04920	XP_003712411.1
Arabidopsis thaliana (thale cress)	AT3G60340	NP_850728.1
Homo sapiens (human)	PPT1	NP_000301.1
Canis lupus familiaris (dog)	PPT1	NP_001010944.1
Bos taurus (cattle)	PPT1	NP_776579.1
Rattus norvegicus (Norway rat)	Ppt1	NP_071947.1
Danio rerio (zebrafish)	ppt1	NP_998504.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001058	XP_311781.4
Pan troglodytes (chimpanzee)	PPT1	XP_001172215.1
Gallus gallus (chicken)	PPT1	NP_001026074.1
Neurospora crassa	NCU06530	XP_961802.1
Oryza sativa (rice)	Os10g0563000	NP_001065397.2
Xenopus tropicalis (tropical clawed frog)	ppt1	NP_001006875.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ppt1 has a role in synaptic vesicle exo- and endocytosis at motor nerve terminals of the Drosophila neuromuscular junction.
Title: Mutations in palmitoyl-protein thioesterase 1 alter exocytosis and endocytosis at synapses in Drosophila larvae.

Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development
Title: The Batten disease Palmitoyl Protein Thioesterase 1 gene regulates neural specification and axon connectivity during Drosophila embryonic development.

An unbiased dominant loss-of-function genetic modifier screen is performed in Drosophila using a previously characterized Ppt1 gain-of-function system.
Title: Identifying cellular pathways modulated by Drosophila palmitoyl-protein thioesterase 1 function.

major lysosomal thioesterase; Ppt2 expression cannot rescue neural inclusion phenotypes associated with loss of Ppt1
Title: The Drosophila protein palmitoylome: characterizing palmitoyl-thioesterases and DHHC palmitoyl-transferases.

Modifier genes identified in this screen connect Ppt1 function to synaptic vesicle cycling, endo-lysosomal trafficking, synaptic development, and activity-dependent remodeling of the synapse.
Title: Genetic modifiers of Drosophila palmitoyl-protein thioesterase 1-induced degeneration.

The following Ppt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03313	NM_167166.4 Latest version!	Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03313	NM_001298095.2 Latest version!	Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppt1 ( NM_167166.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727284.1 Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03313
Clone ID Related Accession (Same CDS sequence)	NM_167166.4 , NM_001298095.2
Accession Version	NM_167166.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Palmitoyl-protein thioesterase 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167166.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTCTA TCTGCTGTTC TCGTTTTTCA TGCATTTTAT TTTTGCTGTT CCTGATCTTT 
TCCCTCGTGC TGAGCTACAT ATGGTGGAGC CCGACGAAAG GAGGAACCAA TCCGGAGGTC 
CTGCCGGTCG TTCTGTGGCA CGGAATGGGC GACACCTGCT GCGTACCCTT CAGCCTGGGT 
GCCATTATGA ACCTGATCGT GGAGCAAACA AAGGGCGGCT ATGTGCGATC CCTGCAGATC 
GGCGGCAATG TTTTGATAGA CTGGCAGAGC GGCTTCTTCA TACATCCCAA CGAACAGGTG 
GATTACGTTT GCAAGCAACT ACTGCAGGAT GAGCACCTGG CCAAGGGATA CCACGCCATC 
GGATTCTCGC AAGGCGGCCA GTTTCTGCGT GCGGTTGCCG AACGTTGCCC GAATCCGCCA 
ATGAGGAACC TTATCACACT CGGTGGGCAG CACCAGGGCA TCTTCGGACT GCCCATGTGC 
CCCACCTTAA CCGAAAAGCC CTGTGATTAT ATCACGAGGC TACTGGACAA CGCCGCCTAC 
GCACCTGAAG TGCAGAAGGC ATTGGTTCAG GCCACCTACT GGCACGACCC GATCATGGAG 
AACAAATACC GCCTGGGCAG CACATTTCTC GCGGACATTA ACAACGAGCT CTTCATCAAC 
AAGTTCTACA TTGAGAATCT GCAAAAGCTT AAGAAATTTG TGATGGTGCA GTTCCTAAAC 
GACACAATTG TCCAGCCGAA GGAGTCCCAG TGGTTTCAAT ACTACACCAC TGGGCAGAAC 
AAAGTCATCC AGCCCTTTAC TGAGAGCAAA GTCTATCAGG ATCTGGGACT GGACAAGATG 
CATCGACAAG GACAACTTGT CTTTCTCGGC GTCGAAGGTG ATCACTTGGC CATATCCAAG 
GCCTGGTTTA TCCAGAACAT AGTGCCTCTC TTACTCGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727284.1
CDS	68..1012
Translation	MISICCSRFSCILFLLFLIFSLVLSYIWWSPTKGGTNPEVLPVVLWHGMGDTCCVPFSLGAIMNLIVEQTKGGYVRSLQIGGNVLIDWQSGFFIHPNEQVDYVCKQLLQDEHLAKGYHAIGFSQGGQFLRAVAERCPNPPMRNLITLGGQHQGIFGLPMCPTLTEKPCDYITRLLDNAAYAPEVQKALVQATYWHDPIMENKYRLGSTFLADINNELFINKFYIENLQKLKKFVMVQFLNDTIVQPKESQWFQYYTTGQNKVIQPFTESKVYQDLGLDKMHRQGQLVFLGVEGDHLAISKAWFIQNIVPLLLEK

Target ORF information:

RefSeq Version	NM_167166.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167166.4

ORF Insert Sequence:

ATGATTTCTA TCTGCTGTTC TCGTTTTTCA TGCATTTTAT TTTTGCTGTT CCTGATCTTT 
TCCCTCGTGC TGAGCTACAT ATGGTGGAGC CCGACGAAAG GAGGAACCAA TCCGGAGGTC 
CTGCCGGTCG TTCTGTGGCA CGGAATGGGC GACACCTGCT GCGTACCCTT CAGCCTGGGT 
GCCATTATGA ACCTGATCGT GGAGCAAACA AAGGGCGGCT ATGTGCGATC CCTGCAGATC 
GGCGGCAATG TTTTGATAGA CTGGCAGAGC GGCTTCTTCA TACATCCCAA CGAACAGGTG 
GATTACGTTT GCAAGCAACT ACTGCAGGAT GAGCACCTGG CCAAGGGATA CCACGCCATC 
GGATTCTCGC AAGGCGGCCA GTTTCTGCGT GCGGTTGCCG AACGTTGCCC GAATCCGCCA 
ATGAGGAACC TTATCACACT CGGTGGGCAG CACCAGGGCA TCTTCGGACT GCCCATGTGC 
CCCACCTTAA CCGAAAAGCC CTGTGATTAT ATCACGAGGC TACTGGACAA CGCCGCCTAC 
GCACCTGAAG TGCAGAAGGC ATTGGTTCAG GCCACCTACT GGCACGACCC GATCATGGAG 
AACAAATACC GCCTGGGCAG CACATTTCTC GCGGACATTA ACAACGAGCT CTTCATCAAC 
AAGTTCTACA TTGAGAATCT GCAAAAGCTT AAGAAATTTG TGATGGTGCA GTTCCTAAAC 
GACACAATTG TCCAGCCGAA GGAGTCCCAG TGGTTTCAAT ACTACACCAC TGGGCAGAAC 
AAAGTCATCC AGCCCTTTAC TGAGAGCAAA GTCTATCAGG ATCTGGGACT GGACAAGATG 
CATCGACAAG GACAACTTGT CTTTCTCGGC GTCGAAGGTG ATCACTTGGC CATATCCAAG 
GCCTGGTTTA TCCAGAACAT AGTGCCTCTC TTACTCGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppt1 ( NM_001298095.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285024.1 Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03313
Clone ID Related Accession (Same CDS sequence)	NM_167166.4 , NM_001298095.2
Accession Version	NM_001298095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Palmitoyl-protein thioesterase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_001298095.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTCTA TCTGCTGTTC TCGTTTTTCA TGCATTTTAT TTTTGCTGTT CCTGATCTTT 
TCCCTCGTGC TGAGCTACAT ATGGTGGAGC CCGACGAAAG GAGGAACCAA TCCGGAGGTC 
CTGCCGGTCG TTCTGTGGCA CGGAATGGGC GACACCTGCT GCGTACCCTT CAGCCTGGGT 
GCCATTATGA ACCTGATCGT GGAGCAAACA AAGGGCGGCT ATGTGCGATC CCTGCAGATC 
GGCGGCAATG TTTTGATAGA CTGGCAGAGC GGCTTCTTCA TACATCCCAA CGAACAGGTG 
GATTACGTTT GCAAGCAACT ACTGCAGGAT GAGCACCTGG CCAAGGGATA CCACGCCATC 
GGATTCTCGC AAGGCGGCCA GTTTCTGCGT GCGGTTGCCG AACGTTGCCC GAATCCGCCA 
ATGAGGAACC TTATCACACT CGGTGGGCAG CACCAGGGCA TCTTCGGACT GCCCATGTGC 
CCCACCTTAA CCGAAAAGCC CTGTGATTAT ATCACGAGGC TACTGGACAA CGCCGCCTAC 
GCACCTGAAG TGCAGAAGGC ATTGGTTCAG GCCACCTACT GGCACGACCC GATCATGGAG 
AACAAATACC GCCTGGGCAG CACATTTCTC GCGGACATTA ACAACGAGCT CTTCATCAAC 
AAGTTCTACA TTGAGAATCT GCAAAAGCTT AAGAAATTTG TGATGGTGCA GTTCCTAAAC 
GACACAATTG TCCAGCCGAA GGAGTCCCAG TGGTTTCAAT ACTACACCAC TGGGCAGAAC 
AAAGTCATCC AGCCCTTTAC TGAGAGCAAA GTCTATCAGG ATCTGGGACT GGACAAGATG 
CATCGACAAG GACAACTTGT CTTTCTCGGC GTCGAAGGTG ATCACTTGGC CATATCCAAG 
GCCTGGTTTA TCCAGAACAT AGTGCCTCTC TTACTCGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285024.1
CDS	189..1133
Translation	MISICCSRFSCILFLLFLIFSLVLSYIWWSPTKGGTNPEVLPVVLWHGMGDTCCVPFSLGAIMNLIVEQTKGGYVRSLQIGGNVLIDWQSGFFIHPNEQVDYVCKQLLQDEHLAKGYHAIGFSQGGQFLRAVAERCPNPPMRNLITLGGQHQGIFGLPMCPTLTEKPCDYITRLLDNAAYAPEVQKALVQATYWHDPIMENKYRLGSTFLADINNELFINKFYIENLQKLKKFVMVQFLNDTIVQPKESQWFQYYTTGQNKVIQPFTESKVYQDLGLDKMHRQGQLVFLGVEGDHLAISKAWFIQNIVPLLLEK

Target ORF information:

RefSeq Version	NM_001298095.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Palmitoyl-protein thioesterase 1 (Ppt1), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298095.2

ORF Insert Sequence:

ATGATTTCTA TCTGCTGTTC TCGTTTTTCA TGCATTTTAT TTTTGCTGTT CCTGATCTTT 
TCCCTCGTGC TGAGCTACAT ATGGTGGAGC CCGACGAAAG GAGGAACCAA TCCGGAGGTC 
CTGCCGGTCG TTCTGTGGCA CGGAATGGGC GACACCTGCT GCGTACCCTT CAGCCTGGGT 
GCCATTATGA ACCTGATCGT GGAGCAAACA AAGGGCGGCT ATGTGCGATC CCTGCAGATC 
GGCGGCAATG TTTTGATAGA CTGGCAGAGC GGCTTCTTCA TACATCCCAA CGAACAGGTG 
GATTACGTTT GCAAGCAACT ACTGCAGGAT GAGCACCTGG CCAAGGGATA CCACGCCATC 
GGATTCTCGC AAGGCGGCCA GTTTCTGCGT GCGGTTGCCG AACGTTGCCC GAATCCGCCA 
ATGAGGAACC TTATCACACT CGGTGGGCAG CACCAGGGCA TCTTCGGACT GCCCATGTGC 
CCCACCTTAA CCGAAAAGCC CTGTGATTAT ATCACGAGGC TACTGGACAA CGCCGCCTAC 
GCACCTGAAG TGCAGAAGGC ATTGGTTCAG GCCACCTACT GGCACGACCC GATCATGGAG 
AACAAATACC GCCTGGGCAG CACATTTCTC GCGGACATTA ACAACGAGCT CTTCATCAAC 
AAGTTCTACA TTGAGAATCT GCAAAAGCTT AAGAAATTTG TGATGGTGCA GTTCCTAAAC 
GACACAATTG TCCAGCCGAA GGAGTCCCAG TGGTTTCAAT ACTACACCAC TGGGCAGAAC 
AAAGTCATCC AGCCCTTTAC TGAGAGCAAA GTCTATCAGG ATCTGGGACT GGACAAGATG 
CATCGACAAG GACAACTTGT CTTTCTCGGC GTCGAAGGTG ATCACTTGGC CATATCCAAG 
GCCTGGTTTA TCCAGAACAT AGTGCCTCTC TTACTCGAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ppt1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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