Miga cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Miga
Entrez Gene ID	31775
Full Name	Mitoguardin
Synonyms	CG12125,Dmel\CG12125,miga
General protein information	Preferred Names Mitoguardin Names CG12125 gene product from transcript CG12125-RC CG12125-PA CG12125-PB CG12125-PC Miga-PA Miga-PB Miga-PC mitoguardin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7E9-7E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_132248.3	NP_572476.2	mitoguardin, isoform A
NM_001169231.2	NP_001162702.1	mitoguardin, isoform B
NM_001298088.1	NP_001285017.1	mitoguardin, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011689	XP_320821.3
Homo sapiens (human)	FAM73B	NP_116198.2
Mus musculus (house mouse)	Fam73b	NP_780601.1
Gallus gallus (chicken)	FAM73B	XP_425337.2
Drosophila melanogaster (fruit fly)	CG12125	NP_001162702.1
Canis lupus familiaris (dog)	FAM73B	XP_005625311.1
Bos taurus (cattle)	FAM73B	NP_001069521.1
Rattus norvegicus (Norway rat)	Fam73b	NP_001100036.1
Pan troglodytes (chimpanzee)	FAM73B	XP_001162600.2
Danio rerio (zebrafish)	fam73b	NP_001013575.1
	LOC722577	XP_001118706.1
Xenopus tropicalis (tropical clawed frog)	fam73b	NP_001096399.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Miga gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Miga cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01112	NM_132248.3 Latest version!	Drosophila melanogaster mitoguardin, transcript variant A (Miga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01112	NM_001169231.2 Latest version!	Drosophila melanogaster mitoguardin, transcript variant B (Miga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01112	NM_001298088.1 Latest version!	Drosophila melanogaster mitoguardin, transcript variant C (Miga), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Miga ( NM_132248.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572476.2 Drosophila melanogaster mitoguardin, transcript variant A (Miga), mRNA.

Price & Availability↑

CloneID	OFa01112
Clone ID Related Accession (Same CDS sequence)	NM_132248.3 , NM_001169231.2 , NM_001298088.1
Accession Version	NM_132248.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitoguardin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132248.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCGCG TCATGCCGGC GTCCTGGAGC AGCGGGGGCA GCCTGCTGCC CTTTCGCGTT 
TCCACCACCA CAAAGGTTGT GCTCTTCTCA CTGACCGCCG GCGTGGCCCT AATGAGCGTA 
CTCTCCCGAT TTCTGCGACG TCGCAAGCCA CCGCGTCCGC CTCGTCGAGC CAGGAAGTAC 
ACGGGTCGGC GCAACCGCAA CAGCATGCGC AGTCCAAACG ATCTAATCTC GATAGCCGGA 
TCTAAGGCCT CGGCCAGATC GGGGAGTCCT GTGGGCTCCA CACTGGCCTA CTCGGATCGC 
CTGTCCATGG CGTCCGGCTC GATTGGCGTT GGTGTATTGG GCGTACAAAA TGCCGGTCCT 
GGCAGCTCGG TAGTCACACA ACTGACGGCC CAACAGTTGG GCATGATGGG CATGGAGGCA 
CTGGACACAG TGATCAACTT CTGGGAGGAT GCCCTGGCGG CACACTACTC GCCCGGCGGT 
TTGCCCGCCC TGTTGACCAC GGCGGAGGAC TCCGAGTTCT GTCGCGAGAT CCAAAACCTG 
CTGGAGATGG CCTACACGCT GCAGGAGCAG AGCGAACTGC TCTTCCTGGA CCAGCGATCG 
GTGCTGTTCC GCGAGGAGCA TTCCATTGAC GAGGCCGAGG AGGAGGCGGG CGAGGCCGAT 
GACGATCGGC GGTCACGCAA ATCGGGCAGC GTTCTCAGTC GCGCTGGTTC CGATCCGAAC 
TTTGATTCCG CGGAAAGCTT TGCTTCCGCT CTCGACCAGG TGGCGGATCT GCGTGAGTTC 
GATGGCTTTA TTGAGACCTC GTACGAAGAG TATCCGCTAT TCCAGAGTGC ACTGAAGCAT 
CACGATGAGT ACACGGTGCC GTGTCGTACC ATTCGGGCGG AGTTGATGCA CTGCAGCACC 
GATACCGAAT ACCTGGCCAA GCTGCACTGC GTTCGGTTGG CCTTTCAGTT CCTCTTCAAG 
GATCCCGCTG TGGGCCAGTG GATCTGCGAT GCTGGTCGGC AGATTCTCAC CGATCTGCTT 
TGTCTGGGCG ATAAGGATAC CAAGGAGTTT CTTGTGGGCT ACGAGGACAT GGTGAACTAT 
CTGCACGATA GCAACAACTG GCCCTGCATC CAAATGGAGC TGGAGCAGCG CAATGTCAAG 
GCGATGACCT TCTACGATAT CTGTTTGGAC TTCATTATCC TCGATTCGTT CAAGGATCTT 
GATGCTCCGC CTGCCAGCGT AACAGCGGTT GTCCAAAATC GCTGGCTATC CAACGGATTC 
AAAGAGACGG CATTAACAAC AGCCGTGTGG TCGGTGCTGA AGGCAAAGAA ACGGATGCTT 
AAGTTCCCCA ACGGGTTCAT GTCCCACTTT TACGTGATAT CCGAGCAGAT ATCACCGCTA 
ATGGCCTGGG GCTTCTTTGG ACCCAACGAG AACTTGCGCG ACATCTGCCA CTACTTCCGA 
GAGGAGCTGC TCGCCTTTCT GGGCGACATC TTTAGCTTCC AAAAGAGCCG CTTCACGACC 
ATCGAGGAAT TCTCGCAGGA TGTGCTGCAG CACATGCAGA CGCGCGTCAA CAACATTGGC 
GTGAAATTTA GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572476.2
CDS	455..2032
Translation	MQRVMPASWSSGGSLLPFRVSTTTKVVLFSLTAGVALMSVLSRFLRRRKPPRPPRRARKYTGRRNRNSMRSPNDLISIAGSKASARSGSPVGSTLAYSDRLSMASGSIGVGVLGVQNAGPGSSVVTQLTAQQLGMMGMEALDTVINFWEDALAAHYSPGGLPALLTTAEDSEFCREIQNLLEMAYTLQEQSELLFLDQRSVLFREEHSIDEAEEEAGEADDDRRSRKSGSVLSRAGSDPNFDSAESFASALDQVADLREFDGFIETSYEEYPLFQSALKHHDEYTVPCRTIRAELMHCSTDTEYLAKLHCVRLAFQFLFKDPAVGQWICDAGRQILTDLLCLGDKDTKEFLVGYEDMVNYLHDSNNWPCIQMELEQRNVKAMTFYDICLDFIILDSFKDLDAPPASVTAVVQNRWLSNGFKETALTTAVWSVLKAKKRMLKFPNGFMSHFYVISEQISPLMAWGFFGPNENLRDICHYFREELLAFLGDIFSFQKSRFTTIEEFSQDVLQHMQTRVNNIGVKFSQ

Target ORF information:

RefSeq Version	NM_132248.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitoguardin, transcript variant A (Miga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132248.3

ORF Insert Sequence:

ATGCAGCGCG TCATGCCGGC GTCCTGGAGC AGCGGGGGCA GCCTGCTGCC CTTTCGCGTT 
TCCACCACCA CAAAGGTTGT GCTCTTCTCA CTGACCGCCG GCGTGGCCCT AATGAGCGTA 
CTCTCCCGAT TTCTGCGACG TCGCAAGCCA CCGCGTCCGC CTCGTCGAGC CAGGAAGTAC 
ACGGGTCGGC GCAACCGCAA CAGCATGCGC AGTCCAAACG ATCTAATCTC GATAGCCGGA 
TCTAAGGCCT CGGCCAGATC GGGGAGTCCT GTGGGCTCCA CACTGGCCTA CTCGGATCGC 
CTGTCCATGG CGTCCGGCTC GATTGGCGTT GGTGTATTGG GCGTACAAAA TGCCGGTCCT 
GGCAGCTCGG TAGTCACACA ACTGACGGCC CAACAGTTGG GCATGATGGG CATGGAGGCA 
CTGGACACAG TGATCAACTT CTGGGAGGAT GCCCTGGCGG CACACTACTC GCCCGGCGGT 
TTGCCCGCCC TGTTGACCAC GGCGGAGGAC TCCGAGTTCT GTCGCGAGAT CCAAAACCTG 
CTGGAGATGG CCTACACGCT GCAGGAGCAG AGCGAACTGC TCTTCCTGGA CCAGCGATCG 
GTGCTGTTCC GCGAGGAGCA TTCCATTGAC GAGGCCGAGG AGGAGGCGGG CGAGGCCGAT 
GACGATCGGC GGTCACGCAA ATCGGGCAGC GTTCTCAGTC GCGCTGGTTC CGATCCGAAC 
TTTGATTCCG CGGAAAGCTT TGCTTCCGCT CTCGACCAGG TGGCGGATCT GCGTGAGTTC 
GATGGCTTTA TTGAGACCTC GTACGAAGAG TATCCGCTAT TCCAGAGTGC ACTGAAGCAT 
CACGATGAGT ACACGGTGCC GTGTCGTACC ATTCGGGCGG AGTTGATGCA CTGCAGCACC 
GATACCGAAT ACCTGGCCAA GCTGCACTGC GTTCGGTTGG CCTTTCAGTT CCTCTTCAAG 
GATCCCGCTG TGGGCCAGTG GATCTGCGAT GCTGGTCGGC AGATTCTCAC CGATCTGCTT 
TGTCTGGGCG ATAAGGATAC CAAGGAGTTT CTTGTGGGCT ACGAGGACAT GGTGAACTAT 
CTGCACGATA GCAACAACTG GCCCTGCATC CAAATGGAGC TGGAGCAGCG CAATGTCAAG 
GCGATGACCT TCTACGATAT CTGTTTGGAC TTCATTATCC TCGATTCGTT CAAGGATCTT 
GATGCTCCGC CTGCCAGCGT AACAGCGGTT GTCCAAAATC GCTGGCTATC CAACGGATTC 
AAAGAGACGG CATTAACAAC AGCCGTGTGG TCGGTGCTGA AGGCAAAGAA ACGGATGCTT 
AAGTTCCCCA ACGGGTTCAT GTCCCACTTT TACGTGATAT CCGAGCAGAT ATCACCGCTA 
ATGGCCTGGG GCTTCTTTGG ACCCAACGAG AACTTGCGCG ACATCTGCCA CTACTTCCGA 
GAGGAGCTGC TCGCCTTTCT GGGCGACATC TTTAGCTTCC AAAAGAGCCG CTTCACGACC 
ATCGAGGAAT TCTCGCAGGA TGTGCTGCAG CACATGCAGA CGCGCGTCAA CAACATTGGC 
GTGAAATTTA GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miga ( NM_001169231.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162702.1 Drosophila melanogaster mitoguardin, transcript variant B (Miga), mRNA.

Price & Availability↑

CloneID	OFa01112
Clone ID Related Accession (Same CDS sequence)	NM_132248.3 , NM_001169231.2 , NM_001298088.1
Accession Version	NM_001169231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitoguardin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169231.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCGCG TCATGCCGGC GTCCTGGAGC AGCGGGGGCA GCCTGCTGCC CTTTCGCGTT 
TCCACCACCA CAAAGGTTGT GCTCTTCTCA CTGACCGCCG GCGTGGCCCT AATGAGCGTA 
CTCTCCCGAT TTCTGCGACG TCGCAAGCCA CCGCGTCCGC CTCGTCGAGC CAGGAAGTAC 
ACGGGTCGGC GCAACCGCAA CAGCATGCGC AGTCCAAACG ATCTAATCTC GATAGCCGGA 
TCTAAGGCCT CGGCCAGATC GGGGAGTCCT GTGGGCTCCA CACTGGCCTA CTCGGATCGC 
CTGTCCATGG CGTCCGGCTC GATTGGCGTT GGTGTATTGG GCGTACAAAA TGCCGGTCCT 
GGCAGCTCGG TAGTCACACA ACTGACGGCC CAACAGTTGG GCATGATGGG CATGGAGGCA 
CTGGACACAG TGATCAACTT CTGGGAGGAT GCCCTGGCGG CACACTACTC GCCCGGCGGT 
TTGCCCGCCC TGTTGACCAC GGCGGAGGAC TCCGAGTTCT GTCGCGAGAT CCAAAACCTG 
CTGGAGATGG CCTACACGCT GCAGGAGCAG AGCGAACTGC TCTTCCTGGA CCAGCGATCG 
GTGCTGTTCC GCGAGGAGCA TTCCATTGAC GAGGCCGAGG AGGAGGCGGG CGAGGCCGAT 
GACGATCGGC GGTCACGCAA ATCGGGCAGC GTTCTCAGTC GCGCTGGTTC CGATCCGAAC 
TTTGATTCCG CGGAAAGCTT TGCTTCCGCT CTCGACCAGG TGGCGGATCT GCGTGAGTTC 
GATGGCTTTA TTGAGACCTC GTACGAAGAG TATCCGCTAT TCCAGAGTGC ACTGAAGCAT 
CACGATGAGT ACACGGTGCC GTGTCGTACC ATTCGGGCGG AGTTGATGCA CTGCAGCACC 
GATACCGAAT ACCTGGCCAA GCTGCACTGC GTTCGGTTGG CCTTTCAGTT CCTCTTCAAG 
GATCCCGCTG TGGGCCAGTG GATCTGCGAT GCTGGTCGGC AGATTCTCAC CGATCTGCTT 
TGTCTGGGCG ATAAGGATAC CAAGGAGTTT CTTGTGGGCT ACGAGGACAT GGTGAACTAT 
CTGCACGATA GCAACAACTG GCCCTGCATC CAAATGGAGC TGGAGCAGCG CAATGTCAAG 
GCGATGACCT TCTACGATAT CTGTTTGGAC TTCATTATCC TCGATTCGTT CAAGGATCTT 
GATGCTCCGC CTGCCAGCGT AACAGCGGTT GTCCAAAATC GCTGGCTATC CAACGGATTC 
AAAGAGACGG CATTAACAAC AGCCGTGTGG TCGGTGCTGA AGGCAAAGAA ACGGATGCTT 
AAGTTCCCCA ACGGGTTCAT GTCCCACTTT TACGTGATAT CCGAGCAGAT ATCACCGCTA 
ATGGCCTGGG GCTTCTTTGG ACCCAACGAG AACTTGCGCG ACATCTGCCA CTACTTCCGA 
GAGGAGCTGC TCGCCTTTCT GGGCGACATC TTTAGCTTCC AAAAGAGCCG CTTCACGACC 
ATCGAGGAAT TCTCGCAGGA TGTGCTGCAG CACATGCAGA CGCGCGTCAA CAACATTGGC 
GTGAAATTTA GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162702.1
CDS	455..2032
Translation	MQRVMPASWSSGGSLLPFRVSTTTKVVLFSLTAGVALMSVLSRFLRRRKPPRPPRRARKYTGRRNRNSMRSPNDLISIAGSKASARSGSPVGSTLAYSDRLSMASGSIGVGVLGVQNAGPGSSVVTQLTAQQLGMMGMEALDTVINFWEDALAAHYSPGGLPALLTTAEDSEFCREIQNLLEMAYTLQEQSELLFLDQRSVLFREEHSIDEAEEEAGEADDDRRSRKSGSVLSRAGSDPNFDSAESFASALDQVADLREFDGFIETSYEEYPLFQSALKHHDEYTVPCRTIRAELMHCSTDTEYLAKLHCVRLAFQFLFKDPAVGQWICDAGRQILTDLLCLGDKDTKEFLVGYEDMVNYLHDSNNWPCIQMELEQRNVKAMTFYDICLDFIILDSFKDLDAPPASVTAVVQNRWLSNGFKETALTTAVWSVLKAKKRMLKFPNGFMSHFYVISEQISPLMAWGFFGPNENLRDICHYFREELLAFLGDIFSFQKSRFTTIEEFSQDVLQHMQTRVNNIGVKFSQ

Target ORF information:

RefSeq Version	NM_001169231.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitoguardin, transcript variant B (Miga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169231.2

ORF Insert Sequence:

ATGCAGCGCG TCATGCCGGC GTCCTGGAGC AGCGGGGGCA GCCTGCTGCC CTTTCGCGTT 
TCCACCACCA CAAAGGTTGT GCTCTTCTCA CTGACCGCCG GCGTGGCCCT AATGAGCGTA 
CTCTCCCGAT TTCTGCGACG TCGCAAGCCA CCGCGTCCGC CTCGTCGAGC CAGGAAGTAC 
ACGGGTCGGC GCAACCGCAA CAGCATGCGC AGTCCAAACG ATCTAATCTC GATAGCCGGA 
TCTAAGGCCT CGGCCAGATC GGGGAGTCCT GTGGGCTCCA CACTGGCCTA CTCGGATCGC 
CTGTCCATGG CGTCCGGCTC GATTGGCGTT GGTGTATTGG GCGTACAAAA TGCCGGTCCT 
GGCAGCTCGG TAGTCACACA ACTGACGGCC CAACAGTTGG GCATGATGGG CATGGAGGCA 
CTGGACACAG TGATCAACTT CTGGGAGGAT GCCCTGGCGG CACACTACTC GCCCGGCGGT 
TTGCCCGCCC TGTTGACCAC GGCGGAGGAC TCCGAGTTCT GTCGCGAGAT CCAAAACCTG 
CTGGAGATGG CCTACACGCT GCAGGAGCAG AGCGAACTGC TCTTCCTGGA CCAGCGATCG 
GTGCTGTTCC GCGAGGAGCA TTCCATTGAC GAGGCCGAGG AGGAGGCGGG CGAGGCCGAT 
GACGATCGGC GGTCACGCAA ATCGGGCAGC GTTCTCAGTC GCGCTGGTTC CGATCCGAAC 
TTTGATTCCG CGGAAAGCTT TGCTTCCGCT CTCGACCAGG TGGCGGATCT GCGTGAGTTC 
GATGGCTTTA TTGAGACCTC GTACGAAGAG TATCCGCTAT TCCAGAGTGC ACTGAAGCAT 
CACGATGAGT ACACGGTGCC GTGTCGTACC ATTCGGGCGG AGTTGATGCA CTGCAGCACC 
GATACCGAAT ACCTGGCCAA GCTGCACTGC GTTCGGTTGG CCTTTCAGTT CCTCTTCAAG 
GATCCCGCTG TGGGCCAGTG GATCTGCGAT GCTGGTCGGC AGATTCTCAC CGATCTGCTT 
TGTCTGGGCG ATAAGGATAC CAAGGAGTTT CTTGTGGGCT ACGAGGACAT GGTGAACTAT 
CTGCACGATA GCAACAACTG GCCCTGCATC CAAATGGAGC TGGAGCAGCG CAATGTCAAG 
GCGATGACCT TCTACGATAT CTGTTTGGAC TTCATTATCC TCGATTCGTT CAAGGATCTT 
GATGCTCCGC CTGCCAGCGT AACAGCGGTT GTCCAAAATC GCTGGCTATC CAACGGATTC 
AAAGAGACGG CATTAACAAC AGCCGTGTGG TCGGTGCTGA AGGCAAAGAA ACGGATGCTT 
AAGTTCCCCA ACGGGTTCAT GTCCCACTTT TACGTGATAT CCGAGCAGAT ATCACCGCTA 
ATGGCCTGGG GCTTCTTTGG ACCCAACGAG AACTTGCGCG ACATCTGCCA CTACTTCCGA 
GAGGAGCTGC TCGCCTTTCT GGGCGACATC TTTAGCTTCC AAAAGAGCCG CTTCACGACC 
ATCGAGGAAT TCTCGCAGGA TGTGCTGCAG CACATGCAGA CGCGCGTCAA CAACATTGGC 
GTGAAATTTA GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Miga ( NM_001298088.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285017.1 Drosophila melanogaster mitoguardin, transcript variant C (Miga), mRNA.

Price & Availability↑

CloneID	OFa01112
Clone ID Related Accession (Same CDS sequence)	NM_132248.3 , NM_001169231.2 , NM_001298088.1
Accession Version	NM_001298088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitoguardin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCGCG TCATGCCGGC GTCCTGGAGC AGCGGGGGCA GCCTGCTGCC CTTTCGCGTT 
TCCACCACCA CAAAGGTTGT GCTCTTCTCA CTGACCGCCG GCGTGGCCCT AATGAGCGTA 
CTCTCCCGAT TTCTGCGACG TCGCAAGCCA CCGCGTCCGC CTCGTCGAGC CAGGAAGTAC 
ACGGGTCGGC GCAACCGCAA CAGCATGCGC AGTCCAAACG ATCTAATCTC GATAGCCGGA 
TCTAAGGCCT CGGCCAGATC GGGGAGTCCT GTGGGCTCCA CACTGGCCTA CTCGGATCGC 
CTGTCCATGG CGTCCGGCTC GATTGGCGTT GGTGTATTGG GCGTACAAAA TGCCGGTCCT 
GGCAGCTCGG TAGTCACACA ACTGACGGCC CAACAGTTGG GCATGATGGG CATGGAGGCA 
CTGGACACAG TGATCAACTT CTGGGAGGAT GCCCTGGCGG CACACTACTC GCCCGGCGGT 
TTGCCCGCCC TGTTGACCAC GGCGGAGGAC TCCGAGTTCT GTCGCGAGAT CCAAAACCTG 
CTGGAGATGG CCTACACGCT GCAGGAGCAG AGCGAACTGC TCTTCCTGGA CCAGCGATCG 
GTGCTGTTCC GCGAGGAGCA TTCCATTGAC GAGGCCGAGG AGGAGGCGGG CGAGGCCGAT 
GACGATCGGC GGTCACGCAA ATCGGGCAGC GTTCTCAGTC GCGCTGGTTC CGATCCGAAC 
TTTGATTCCG CGGAAAGCTT TGCTTCCGCT CTCGACCAGG TGGCGGATCT GCGTGAGTTC 
GATGGCTTTA TTGAGACCTC GTACGAAGAG TATCCGCTAT TCCAGAGTGC ACTGAAGCAT 
CACGATGAGT ACACGGTGCC GTGTCGTACC ATTCGGGCGG AGTTGATGCA CTGCAGCACC 
GATACCGAAT ACCTGGCCAA GCTGCACTGC GTTCGGTTGG CCTTTCAGTT CCTCTTCAAG 
GATCCCGCTG TGGGCCAGTG GATCTGCGAT GCTGGTCGGC AGATTCTCAC CGATCTGCTT 
TGTCTGGGCG ATAAGGATAC CAAGGAGTTT CTTGTGGGCT ACGAGGACAT GGTGAACTAT 
CTGCACGATA GCAACAACTG GCCCTGCATC CAAATGGAGC TGGAGCAGCG CAATGTCAAG 
GCGATGACCT TCTACGATAT CTGTTTGGAC TTCATTATCC TCGATTCGTT CAAGGATCTT 
GATGCTCCGC CTGCCAGCGT AACAGCGGTT GTCCAAAATC GCTGGCTATC CAACGGATTC 
AAAGAGACGG CATTAACAAC AGCCGTGTGG TCGGTGCTGA AGGCAAAGAA ACGGATGCTT 
AAGTTCCCCA ACGGGTTCAT GTCCCACTTT TACGTGATAT CCGAGCAGAT ATCACCGCTA 
ATGGCCTGGG GCTTCTTTGG ACCCAACGAG AACTTGCGCG ACATCTGCCA CTACTTCCGA 
GAGGAGCTGC TCGCCTTTCT GGGCGACATC TTTAGCTTCC AAAAGAGCCG CTTCACGACC 
ATCGAGGAAT TCTCGCAGGA TGTGCTGCAG CACATGCAGA CGCGCGTCAA CAACATTGGC 
GTGAAATTTA GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285017.1
CDS	1474..3051
Translation	MQRVMPASWSSGGSLLPFRVSTTTKVVLFSLTAGVALMSVLSRFLRRRKPPRPPRRARKYTGRRNRNSMRSPNDLISIAGSKASARSGSPVGSTLAYSDRLSMASGSIGVGVLGVQNAGPGSSVVTQLTAQQLGMMGMEALDTVINFWEDALAAHYSPGGLPALLTTAEDSEFCREIQNLLEMAYTLQEQSELLFLDQRSVLFREEHSIDEAEEEAGEADDDRRSRKSGSVLSRAGSDPNFDSAESFASALDQVADLREFDGFIETSYEEYPLFQSALKHHDEYTVPCRTIRAELMHCSTDTEYLAKLHCVRLAFQFLFKDPAVGQWICDAGRQILTDLLCLGDKDTKEFLVGYEDMVNYLHDSNNWPCIQMELEQRNVKAMTFYDICLDFIILDSFKDLDAPPASVTAVVQNRWLSNGFKETALTTAVWSVLKAKKRMLKFPNGFMSHFYVISEQISPLMAWGFFGPNENLRDICHYFREELLAFLGDIFSFQKSRFTTIEEFSQDVLQHMQTRVNNIGVKFSQ

Target ORF information:

RefSeq Version	NM_001298088.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitoguardin, transcript variant C (Miga), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298088.1

ORF Insert Sequence:

ATGCAGCGCG TCATGCCGGC GTCCTGGAGC AGCGGGGGCA GCCTGCTGCC CTTTCGCGTT 
TCCACCACCA CAAAGGTTGT GCTCTTCTCA CTGACCGCCG GCGTGGCCCT AATGAGCGTA 
CTCTCCCGAT TTCTGCGACG TCGCAAGCCA CCGCGTCCGC CTCGTCGAGC CAGGAAGTAC 
ACGGGTCGGC GCAACCGCAA CAGCATGCGC AGTCCAAACG ATCTAATCTC GATAGCCGGA 
TCTAAGGCCT CGGCCAGATC GGGGAGTCCT GTGGGCTCCA CACTGGCCTA CTCGGATCGC 
CTGTCCATGG CGTCCGGCTC GATTGGCGTT GGTGTATTGG GCGTACAAAA TGCCGGTCCT 
GGCAGCTCGG TAGTCACACA ACTGACGGCC CAACAGTTGG GCATGATGGG CATGGAGGCA 
CTGGACACAG TGATCAACTT CTGGGAGGAT GCCCTGGCGG CACACTACTC GCCCGGCGGT 
TTGCCCGCCC TGTTGACCAC GGCGGAGGAC TCCGAGTTCT GTCGCGAGAT CCAAAACCTG 
CTGGAGATGG CCTACACGCT GCAGGAGCAG AGCGAACTGC TCTTCCTGGA CCAGCGATCG 
GTGCTGTTCC GCGAGGAGCA TTCCATTGAC GAGGCCGAGG AGGAGGCGGG CGAGGCCGAT 
GACGATCGGC GGTCACGCAA ATCGGGCAGC GTTCTCAGTC GCGCTGGTTC CGATCCGAAC 
TTTGATTCCG CGGAAAGCTT TGCTTCCGCT CTCGACCAGG TGGCGGATCT GCGTGAGTTC 
GATGGCTTTA TTGAGACCTC GTACGAAGAG TATCCGCTAT TCCAGAGTGC ACTGAAGCAT 
CACGATGAGT ACACGGTGCC GTGTCGTACC ATTCGGGCGG AGTTGATGCA CTGCAGCACC 
GATACCGAAT ACCTGGCCAA GCTGCACTGC GTTCGGTTGG CCTTTCAGTT CCTCTTCAAG 
GATCCCGCTG TGGGCCAGTG GATCTGCGAT GCTGGTCGGC AGATTCTCAC CGATCTGCTT 
TGTCTGGGCG ATAAGGATAC CAAGGAGTTT CTTGTGGGCT ACGAGGACAT GGTGAACTAT 
CTGCACGATA GCAACAACTG GCCCTGCATC CAAATGGAGC TGGAGCAGCG CAATGTCAAG 
GCGATGACCT TCTACGATAT CTGTTTGGAC TTCATTATCC TCGATTCGTT CAAGGATCTT 
GATGCTCCGC CTGCCAGCGT AACAGCGGTT GTCCAAAATC GCTGGCTATC CAACGGATTC 
AAAGAGACGG CATTAACAAC AGCCGTGTGG TCGGTGCTGA AGGCAAAGAA ACGGATGCTT 
AAGTTCCCCA ACGGGTTCAT GTCCCACTTT TACGTGATAT CCGAGCAGAT ATCACCGCTA 
ATGGCCTGGG GCTTCTTTGG ACCCAACGAG AACTTGCGCG ACATCTGCCA CTACTTCCGA 
GAGGAGCTGC TCGCCTTTCT GGGCGACATC TTTAGCTTCC AAAAGAGCCG CTTCACGACC 
ATCGAGGAAT TCTCGCAGGA TGTGCTGCAG CACATGCAGA CGCGCGTCAA CAACATTGGC 
GTGAAATTTA GCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Miga cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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