Trf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trf2
Entrez Gene ID	31773
Full Name	TATA box binding protein-related factor 2
Synonyms	CG11195,CG18009,Dmel\CG18009,TLF,TRF2,dTRF2,dTRF2L,dTRF2S,dTrf2,l(1)G0039,l(1)G0071,l(1)G0295,l(1)G0425,p79/TRF2,trf2
General protein information	Preferred Names TATA box binding protein-related factor 2 Names CG18009 gene product from transcript CG18009-RH CG18009-PE CG18009-PF CG18009-PG CG18009-PH CG18009-PI CG18009-PJ TATA box-binding protein related factor 2 TBP related factor 2 TBP-like factor TBP-related factor 2 Trf2-PE Trf2-PF Trf2-PG Trf2-PH Trf2-PI Trf2-PJ lethal (1) G0039 lethal (1) G0071 lethal (1) G0295 lethal (1) G0425
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7E7-7E

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272401.1	NP_001259330.1	TATA box binding protein-related factor 2, isoform G
NM_001103435.3	NP_001096905.1	TATA box binding protein-related factor 2, isoform E
NM_078529.4	NP_511084.2	TATA box binding protein-related factor 2, isoform J
NM_001169230.2	NP_001162701.1	TATA box binding protein-related factor 2, isoform F
NM_001272400.1	NP_001259329.1	TATA box binding protein-related factor 2, isoform I
NM_206654.2	NP_996377.1	TATA box binding protein-related factor 2, isoform H

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Although M1BP associates with thousands of genes, its colocalization with TRF2 is largely restricted to Ribosomal proteins genes, suggesting that this combination is key to coordinately regulating transcription of the majority of Ribosomal Proteins genes in Drosophila.
Title: A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes.

TRF2 and TAF9 have roles in regulating lipid droplet size and phospholipid fatty acid composition
Title: Drosophila TRF2 and TAF9 regulate lipid droplet size and phospholipid fatty acid composition.

the role of short TRF2 as a preferential core promoter regulator.
Title: Drosophila TRF2 is a preferential core promoter regulator.

specialized TRF2-based RNA polymerase II system functions in the synthesis of RPs and complements the RNA polymerase I and III systems
Title: TRF2, but not TBP, mediates the transcription of ribosomal protein genes.

Suppression of the TRF2 expression violates the development of a compact chromocenter and the correct approach of homologous chromosomes (in germinative and somatic cells).
Title: [Effect of mutations in lawc/Trf2 gene on development of chromocenter and chromosome disjunction in Drosophila melanogaster].

The following Trf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01110	NM_078529.4 Latest version!	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01110	NM_206654.2 Latest version!	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa01111	NM_001103435.3 Latest version!	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa01111	NM_001272401.1 Latest version!	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa01111	NM_001169230.2 Latest version!	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa03282	NM_001272400.1 Latest version!	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trf2 ( NM_078529.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511084.2 Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa01110
Clone ID Related Accession (Same CDS sequence)	NM_078529.4 , NM_206654.2
Accession Version	NM_078529.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TATA box binding protein-related factor 2, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078529.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAACG ATATGGTCAG CATACCGGTG GCCAATTTGA ACGGCGGCCT TAAAGCGGCC 
AGCAGCGGTT CCGGAGTTGG TGTAGTGACC TCCGGCGGCG TCGTATCCTC GGCGGTGCTG 
GCCAATGCCC CACGTGTATA CCTAACGCCC TCCTCCACAT TCATGACCAA TCGCCAAATG 
GCGGGAGTGG CGTCCACCGG AAGAATGAGC GGACAAGTCG TCGGTGGTTC ATCTGGCACA 
GCCTCAACAG CCGGTACCGT TCGCTATTTT TCGCAATTCA GCAAAATGCA AACGGCCGGC 
GGACCCAGTT TGCAAAGGAA ACTTGCCAAC GGGGATACCA TTGTTCTGGC AACCGGCAGT 
AAAAATATGT TCCTTACTAG CAGCGAAAAT AAGGCTAATC TGCCGACCGT CGCCAGCAAT 
GGCAATGGTC TGATCACAGC CAAAATGGAT CTATTGGAGG AGGAGGTCAT GCAGTCGATA 
ACTGTGATTG ATGACGATGA CGAGGAGAAA AAGGAAGTCG CTGAAGACGA AGAAGAGAGC 
AGCAATAATG CCAAACCCAT TGACCTTCAC CAGCCAATTG CTGATAATGA GCACGAGCTG 
GACATTGTGA TAAACAATGT GGTCTGCTCG TTTAGCGTCG GCTGTCACCT GAAGCTGCGC 
GAAATTGCGC TGCAGGGATC AAATGTGGAG TACCGTCGCG AGAACGGAAT GGTGACAATG 
AAGCTCCGCC ATCCATACAC CACTGCCTCG ATTTGGTCGT CGGGGAGGAT TACCTGCACT 
GGTGCCACTT CCGAGTCAAT GGCCAAGGTC GCTGCACGCC GCTATGCCAG ATGCCTTGGC 
AAGCTGGGAT TTCCCACTCG TTTCCTCAAC TTTCGCATTG TCAACGTACT GGGCACCTGC 
AGCATGCCGT GGGCCATCAA GATCGTCAAC TTTTCGGAGC GCCATCGCGA GAACGCCAGC 
TACGAGCCCG AGCTGCATCC TGGAGTGACT TACAAGATGC GCGATCCCGA TCCAAAGGCC 
ACCCTGAAGA TCTTCTCCAC CGGCAGCGTA ACAGTCACCG CGGCTAGCGT AAACCACGTT 
GAATCGGCTA TCCAGCACAT ATACCCGCTG GTCTTTGATT TCCGCAAGCA ACGTTCGGCG 
GAGGAGCTGC AGCATCTGCG TCAAAAGCAG CGTCTGCAAG CCGGCGGTGA TCCCCACGAA 
CTGGAGAAAA ATGTCTTGGC CGATAACAAG ACTGCTTCCC TTGATAATAT CTTTGTTAAT 
ACAACAGCGG CGCACTCTAA ATCATCGTCG AATGACCAAA CATCAGCACC CGCGACCATC 
TTGTCCAGCA CAGTTGATAG TATGCCTCGT CTGAAACAGA TGGTCAACTA TCACCAGATG 
ATGAAGCAAA CCCAGGAAGA GCGCCGCCAT ATAATGTTTA ATGGGGAGAA GGCCAATCCG 
GCCTCCACCT CATCGGCGGC AGCAGCTCCC TCCACCTCCT CCTCATCGTC CTCATCCGGG 
GATAATATCT GTGCCAATGC CCGTCGCCGG GCCACCGAAT GCTGGGCAAC CAAGCTGCAG 
AATAAGCGTC CACGTTACAA TGATCCCGGT ACCACGGGCA CAATTAACGC CGCATCGTCC 
ACAGCATCAG CCGCCACATC TTCGTTAGCC TCCCAGGCGA CACATCTGCG TAATCCTCTC 
AAGACGGCCG CGTTGGCCAA TGCACGTATG TTAGGCGCCA AGGTGACAAC ATGCACACGC 
AACAGCATAA TCGTGCAGCA GCCGCAACGT ATCCAAATGC AGCAGCAGCA GCAACAGCTG 
CAACCGCAGC AGCAACAGAC CAGCTTCTCG CCCAGCGAAT TCGATGTTGA TGACTTGATC 
GAGGAGGAGG AGAACAACGA ATTGGATATG CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511084.2
CDS	4016..5914
Translation	MQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTNRQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLATGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDEEESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVLGTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASVNHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNIFVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEKANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINAASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQQQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF

Target ORF information:

RefSeq Version	NM_078529.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078529.4

ORF Insert Sequence:

ATGCAAAACG ATATGGTCAG CATACCGGTG GCCAATTTGA ACGGCGGCCT TAAAGCGGCC 
AGCAGCGGTT CCGGAGTTGG TGTAGTGACC TCCGGCGGCG TCGTATCCTC GGCGGTGCTG 
GCCAATGCCC CACGTGTATA CCTAACGCCC TCCTCCACAT TCATGACCAA TCGCCAAATG 
GCGGGAGTGG CGTCCACCGG AAGAATGAGC GGACAAGTCG TCGGTGGTTC ATCTGGCACA 
GCCTCAACAG CCGGTACCGT TCGCTATTTT TCGCAATTCA GCAAAATGCA AACGGCCGGC 
GGACCCAGTT TGCAAAGGAA ACTTGCCAAC GGGGATACCA TTGTTCTGGC AACCGGCAGT 
AAAAATATGT TCCTTACTAG CAGCGAAAAT AAGGCTAATC TGCCGACCGT CGCCAGCAAT 
GGCAATGGTC TGATCACAGC CAAAATGGAT CTATTGGAGG AGGAGGTCAT GCAGTCGATA 
ACTGTGATTG ATGACGATGA CGAGGAGAAA AAGGAAGTCG CTGAAGACGA AGAAGAGAGC 
AGCAATAATG CCAAACCCAT TGACCTTCAC CAGCCAATTG CTGATAATGA GCACGAGCTG 
GACATTGTGA TAAACAATGT GGTCTGCTCG TTTAGCGTCG GCTGTCACCT GAAGCTGCGC 
GAAATTGCGC TGCAGGGATC AAATGTGGAG TACCGTCGCG AGAACGGAAT GGTGACAATG 
AAGCTCCGCC ATCCATACAC CACTGCCTCG ATTTGGTCGT CGGGGAGGAT TACCTGCACT 
GGTGCCACTT CCGAGTCAAT GGCCAAGGTC GCTGCACGCC GCTATGCCAG ATGCCTTGGC 
AAGCTGGGAT TTCCCACTCG TTTCCTCAAC TTTCGCATTG TCAACGTACT GGGCACCTGC 
AGCATGCCGT GGGCCATCAA GATCGTCAAC TTTTCGGAGC GCCATCGCGA GAACGCCAGC 
TACGAGCCCG AGCTGCATCC TGGAGTGACT TACAAGATGC GCGATCCCGA TCCAAAGGCC 
ACCCTGAAGA TCTTCTCCAC CGGCAGCGTA ACAGTCACCG CGGCTAGCGT AAACCACGTT 
GAATCGGCTA TCCAGCACAT ATACCCGCTG GTCTTTGATT TCCGCAAGCA ACGTTCGGCG 
GAGGAGCTGC AGCATCTGCG TCAAAAGCAG CGTCTGCAAG CCGGCGGTGA TCCCCACGAA 
CTGGAGAAAA ATGTCTTGGC CGATAACAAG ACTGCTTCCC TTGATAATAT CTTTGTTAAT 
ACAACAGCGG CGCACTCTAA ATCATCGTCG AATGACCAAA CATCAGCACC CGCGACCATC 
TTGTCCAGCA CAGTTGATAG TATGCCTCGT CTGAAACAGA TGGTCAACTA TCACCAGATG 
ATGAAGCAAA CCCAGGAAGA GCGCCGCCAT ATAATGTTTA ATGGGGAGAA GGCCAATCCG 
GCCTCCACCT CATCGGCGGC AGCAGCTCCC TCCACCTCCT CCTCATCGTC CTCATCCGGG 
GATAATATCT GTGCCAATGC CCGTCGCCGG GCCACCGAAT GCTGGGCAAC CAAGCTGCAG 
AATAAGCGTC CACGTTACAA TGATCCCGGT ACCACGGGCA CAATTAACGC CGCATCGTCC 
ACAGCATCAG CCGCCACATC TTCGTTAGCC TCCCAGGCGA CACATCTGCG TAATCCTCTC 
AAGACGGCCG CGTTGGCCAA TGCACGTATG TTAGGCGCCA AGGTGACAAC ATGCACACGC 
AACAGCATAA TCGTGCAGCA GCCGCAACGT ATCCAAATGC AGCAGCAGCA GCAACAGCTG 
CAACCGCAGC AGCAACAGAC CAGCTTCTCG CCCAGCGAAT TCGATGTTGA TGACTTGATC 
GAGGAGGAGG AGAACAACGA ATTGGATATG CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf2 ( NM_206654.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996377.1 Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa01110
Clone ID Related Accession (Same CDS sequence)	NM_078529.4 , NM_206654.2
Accession Version	NM_206654.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TATA box binding protein-related factor 2, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_206654.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAACG ATATGGTCAG CATACCGGTG GCCAATTTGA ACGGCGGCCT TAAAGCGGCC 
AGCAGCGGTT CCGGAGTTGG TGTAGTGACC TCCGGCGGCG TCGTATCCTC GGCGGTGCTG 
GCCAATGCCC CACGTGTATA CCTAACGCCC TCCTCCACAT TCATGACCAA TCGCCAAATG 
GCGGGAGTGG CGTCCACCGG AAGAATGAGC GGACAAGTCG TCGGTGGTTC ATCTGGCACA 
GCCTCAACAG CCGGTACCGT TCGCTATTTT TCGCAATTCA GCAAAATGCA AACGGCCGGC 
GGACCCAGTT TGCAAAGGAA ACTTGCCAAC GGGGATACCA TTGTTCTGGC AACCGGCAGT 
AAAAATATGT TCCTTACTAG CAGCGAAAAT AAGGCTAATC TGCCGACCGT CGCCAGCAAT 
GGCAATGGTC TGATCACAGC CAAAATGGAT CTATTGGAGG AGGAGGTCAT GCAGTCGATA 
ACTGTGATTG ATGACGATGA CGAGGAGAAA AAGGAAGTCG CTGAAGACGA AGAAGAGAGC 
AGCAATAATG CCAAACCCAT TGACCTTCAC CAGCCAATTG CTGATAATGA GCACGAGCTG 
GACATTGTGA TAAACAATGT GGTCTGCTCG TTTAGCGTCG GCTGTCACCT GAAGCTGCGC 
GAAATTGCGC TGCAGGGATC AAATGTGGAG TACCGTCGCG AGAACGGAAT GGTGACAATG 
AAGCTCCGCC ATCCATACAC CACTGCCTCG ATTTGGTCGT CGGGGAGGAT TACCTGCACT 
GGTGCCACTT CCGAGTCAAT GGCCAAGGTC GCTGCACGCC GCTATGCCAG ATGCCTTGGC 
AAGCTGGGAT TTCCCACTCG TTTCCTCAAC TTTCGCATTG TCAACGTACT GGGCACCTGC 
AGCATGCCGT GGGCCATCAA GATCGTCAAC TTTTCGGAGC GCCATCGCGA GAACGCCAGC 
TACGAGCCCG AGCTGCATCC TGGAGTGACT TACAAGATGC GCGATCCCGA TCCAAAGGCC 
ACCCTGAAGA TCTTCTCCAC CGGCAGCGTA ACAGTCACCG CGGCTAGCGT AAACCACGTT 
GAATCGGCTA TCCAGCACAT ATACCCGCTG GTCTTTGATT TCCGCAAGCA ACGTTCGGCG 
GAGGAGCTGC AGCATCTGCG TCAAAAGCAG CGTCTGCAAG CCGGCGGTGA TCCCCACGAA 
CTGGAGAAAA ATGTCTTGGC CGATAACAAG ACTGCTTCCC TTGATAATAT CTTTGTTAAT 
ACAACAGCGG CGCACTCTAA ATCATCGTCG AATGACCAAA CATCAGCACC CGCGACCATC 
TTGTCCAGCA CAGTTGATAG TATGCCTCGT CTGAAACAGA TGGTCAACTA TCACCAGATG 
ATGAAGCAAA CCCAGGAAGA GCGCCGCCAT ATAATGTTTA ATGGGGAGAA GGCCAATCCG 
GCCTCCACCT CATCGGCGGC AGCAGCTCCC TCCACCTCCT CCTCATCGTC CTCATCCGGG 
GATAATATCT GTGCCAATGC CCGTCGCCGG GCCACCGAAT GCTGGGCAAC CAAGCTGCAG 
AATAAGCGTC CACGTTACAA TGATCCCGGT ACCACGGGCA CAATTAACGC CGCATCGTCC 
ACAGCATCAG CCGCCACATC TTCGTTAGCC TCCCAGGCGA CACATCTGCG TAATCCTCTC 
AAGACGGCCG CGTTGGCCAA TGCACGTATG TTAGGCGCCA AGGTGACAAC ATGCACACGC 
AACAGCATAA TCGTGCAGCA GCCGCAACGT ATCCAAATGC AGCAGCAGCA GCAACAGCTG 
CAACCGCAGC AGCAACAGAC CAGCTTCTCG CCCAGCGAAT TCGATGTTGA TGACTTGATC 
GAGGAGGAGG AGAACAACGA ATTGGATATG CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996377.1
CDS	4307..6205
Translation	MQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTNRQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLATGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDEEESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVLGTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASVNHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNIFVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEKANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINAASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQQQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF

Target ORF information:

RefSeq Version	NM_206654.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206654.2

ORF Insert Sequence:

ATGCAAAACG ATATGGTCAG CATACCGGTG GCCAATTTGA ACGGCGGCCT TAAAGCGGCC 
AGCAGCGGTT CCGGAGTTGG TGTAGTGACC TCCGGCGGCG TCGTATCCTC GGCGGTGCTG 
GCCAATGCCC CACGTGTATA CCTAACGCCC TCCTCCACAT TCATGACCAA TCGCCAAATG 
GCGGGAGTGG CGTCCACCGG AAGAATGAGC GGACAAGTCG TCGGTGGTTC ATCTGGCACA 
GCCTCAACAG CCGGTACCGT TCGCTATTTT TCGCAATTCA GCAAAATGCA AACGGCCGGC 
GGACCCAGTT TGCAAAGGAA ACTTGCCAAC GGGGATACCA TTGTTCTGGC AACCGGCAGT 
AAAAATATGT TCCTTACTAG CAGCGAAAAT AAGGCTAATC TGCCGACCGT CGCCAGCAAT 
GGCAATGGTC TGATCACAGC CAAAATGGAT CTATTGGAGG AGGAGGTCAT GCAGTCGATA 
ACTGTGATTG ATGACGATGA CGAGGAGAAA AAGGAAGTCG CTGAAGACGA AGAAGAGAGC 
AGCAATAATG CCAAACCCAT TGACCTTCAC CAGCCAATTG CTGATAATGA GCACGAGCTG 
GACATTGTGA TAAACAATGT GGTCTGCTCG TTTAGCGTCG GCTGTCACCT GAAGCTGCGC 
GAAATTGCGC TGCAGGGATC AAATGTGGAG TACCGTCGCG AGAACGGAAT GGTGACAATG 
AAGCTCCGCC ATCCATACAC CACTGCCTCG ATTTGGTCGT CGGGGAGGAT TACCTGCACT 
GGTGCCACTT CCGAGTCAAT GGCCAAGGTC GCTGCACGCC GCTATGCCAG ATGCCTTGGC 
AAGCTGGGAT TTCCCACTCG TTTCCTCAAC TTTCGCATTG TCAACGTACT GGGCACCTGC 
AGCATGCCGT GGGCCATCAA GATCGTCAAC TTTTCGGAGC GCCATCGCGA GAACGCCAGC 
TACGAGCCCG AGCTGCATCC TGGAGTGACT TACAAGATGC GCGATCCCGA TCCAAAGGCC 
ACCCTGAAGA TCTTCTCCAC CGGCAGCGTA ACAGTCACCG CGGCTAGCGT AAACCACGTT 
GAATCGGCTA TCCAGCACAT ATACCCGCTG GTCTTTGATT TCCGCAAGCA ACGTTCGGCG 
GAGGAGCTGC AGCATCTGCG TCAAAAGCAG CGTCTGCAAG CCGGCGGTGA TCCCCACGAA 
CTGGAGAAAA ATGTCTTGGC CGATAACAAG ACTGCTTCCC TTGATAATAT CTTTGTTAAT 
ACAACAGCGG CGCACTCTAA ATCATCGTCG AATGACCAAA CATCAGCACC CGCGACCATC 
TTGTCCAGCA CAGTTGATAG TATGCCTCGT CTGAAACAGA TGGTCAACTA TCACCAGATG 
ATGAAGCAAA CCCAGGAAGA GCGCCGCCAT ATAATGTTTA ATGGGGAGAA GGCCAATCCG 
GCCTCCACCT CATCGGCGGC AGCAGCTCCC TCCACCTCCT CCTCATCGTC CTCATCCGGG 
GATAATATCT GTGCCAATGC CCGTCGCCGG GCCACCGAAT GCTGGGCAAC CAAGCTGCAG 
AATAAGCGTC CACGTTACAA TGATCCCGGT ACCACGGGCA CAATTAACGC CGCATCGTCC 
ACAGCATCAG CCGCCACATC TTCGTTAGCC TCCCAGGCGA CACATCTGCG TAATCCTCTC 
AAGACGGCCG CGTTGGCCAA TGCACGTATG TTAGGCGCCA AGGTGACAAC ATGCACACGC 
AACAGCATAA TCGTGCAGCA GCCGCAACGT ATCCAAATGC AGCAGCAGCA GCAACAGCTG 
CAACCGCAGC AGCAACAGAC CAGCTTCTCG CCCAGCGAAT TCGATGTTGA TGACTTGATC 
GAGGAGGAGG AGAACAACGA ATTGGATATG CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf2 ( NM_001272401.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259330.1 Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa01111
Clone ID Related Accession (Same CDS sequence)	NM_001272401.1 , NM_001103435.3 , NM_001169230.2
Accession Version	NM_001272401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TATA box binding protein-related factor 2, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAGTGAT 
CGCTTAAGTT CTTCTAATAG CGAGGACGAA CAGCTGCACC AAGAATATTC AACTTCATCA 
AGAGGAAGAA CAAGGGAATA TGCACAATCT GACTTGAATC CCGGGTACCA GGACTTGAAT 
ATCGGTAACC AGGACTTGAA TCAAGTTAAT CGCGAGTACC AGGAGTCTAA TAACGAGTAC 
CAGGTTTCAA ACCAAGAGTA CCAGCACTTG AATACCGAGT ACCAGGAGTT GAATCACGGC 
GATCAGGACT CAAATGACGA GTACCAGGAG TTGAATCACG GCAACCAGGA CTCAAATGAC 
GAGTACCAGG AATCAAATCA CGGCAACCAG GACTCAAATG ACGAGTACCA GGAGTTGAAT 
CACAGCAACC AGGACTCAAA TGACGAGTAC CAGGAGTTGA ATCACGGCGA TCAGGACTCA 
AGTGACGAGT ACCAGGATTT GAATCACGTC AACCAAGACT CAAATCACGA GTACCAGGCT 
TGGAATCACG AATACCAGGA CTCAAATCAC GAGTATCAGG CCTTGAATCG AGAGTACCAA 
TATCCTGAAT CTGATGAGGA AGAGGCCGCT AGAAAAAGAA ACATATATAC CCGTTTACGA 
TCCTACGCTT GTCAGGTACA TCTCCCTCGT TTAGGAGAAT CAGAAGTTGA TAGCGACGAT 
CGTCGCCATA ACCCAATCAA TCAGCCATTC AATCAGCCAA TCAATAGGCC AAATCAAAAA 
TCGGATTCAT CTTTAGAGTC ATCTTCGCCG GAGAGACAAG ACTCTGAAAG CGAGTCGCGA 
CAAGCTTCAG ATGAGGAATC GTCTTCTGGG GACTCGGACT ACTTAATCGA TCCAGTTACT 
GGGTGGCTAT CGCGCCGGCG AGAAACTTCT ACCGAACGCG AGGAAAAAAG CGACCAAAGC 
CAACAGGAGT CGTCAGAGGA AATTACAGAC GAACAGAAGG AAGAAAGCGA CGACGTCGAA 
CAACAGTCGC CGGCTGAACA TACTTCTTCT GATAGACGCG ATCATTCGCC AGACAAGCAA 
CAGAATAAGC GAAAATCGTT AGGCACTGGG AATTCTTCGT ATTCGAAGAA ACCATTATTG 
GACGGTGACA AAGGAGACCC ACGTTCACAT TTATCGTCTC TGGGAAGTCG CAGATCCTTA 
AAGCGTAAAT GGCCTTTTGA TTGTACAGAC TCTAAGAAAT TTAAACCAAA CTTGCAGAGT 
GAGGAAGACA AATCTGAGGA GGAAAAACGT GAAGATACGG AAAGCGAATG CTCTGACGAA 
GAAGACTCAG ATAGTTCAAG TTTCGATACA GATTCAAATT CAGATTCAGA TTCAAATTTA 
GATTTAGATT CCGATTCCGA TTCCCATTCC GATTTCGAAT CATCTTCAAG TTCTGAGGAA 
TCTGCCCAGG ATCCAGCTCC TGAAGGCGAA ACCGATCAAG ATCCAGCTCC TGAAGGCGAA 
GCCGATCAAG ATCCAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAAGGCGGA 
GTCGATCAAG ATCCAGCTCC TGAAGGCGAA GTCGATCAAG ATCCAGCTCC TGAAGGCGAA 
TCCGATCAAG ATCTAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAATTCGGA 
ACCGATCAAG ATCCACATCC TTATTACAAC GATCAGGATC CAGCTCCTTA TTACGACGAT 
CAGGATCCAG CTTCTTATTA CGACGATCAC ATTTGTGATT CTGAGGAGGA TTATAAACCA 
TTTATCTTCT CAAAGGAAAG GAAGTTCTTG AAATTCGTAA CAAATCCTTT AGGGGATGAT 
AAATCTAGTT TGGACCTTAG CATACAGCTC TATAAGGTAT TTGGAGCTTA CAAACTGGAA 
GACGTATACA GCAGTATTGA TGAGGAAGGC CAACCAGAAC AGGACCTGCA ACTGAAGCAG 
CAACAGAAAC ATTTAAAAAA ATATCAAAAG TTGCAACTGG TGGTATTCTG GCACCAGGAG 
AAATTCTTTT CAAAGGAGCA ACTGTTGCTA TATGAGTATC TGAAACTACT TTTGCAGAAA 
TTCTTTCCAC AAGAGCAACA GGAGAAACGG TTGCAACAGG AGAAACTTTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACGGTTGCAA CAGGAGAAAC GGTTGCAACA GGAGAAACGG 
TTGCAACAGG AGAAACGGTT GCAACAGGAG AAACGGTTGC AACAGCAGAA ACTCTTGCAA 
CAGCAGAAAC TCTTGCAACA GGAGAAACTC TTGCAACAGC AGAAACTCTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACTCTTGCAA CAGGAGAAAC AATTGCAACA GGAGAAACAC 
TTGCCACAGG AGCGACAGTT GCAACAGGAT AAAGTCTTCC TACAGCTACG ACAGTTGCAA 
CAGAAGAAAG AACTGGAGGA GCAGCGAGAG CGAAAGCGCC ACCTCTTTGA GCAACAGAAA 
CAGTTTGTCC TAGAAAAATT GCGCCAACAG AAGGAGCAGC ATCGTCGTCA GCAAAATCGC 
GTAAAACGCG AACGTAAATT GCGCCAGAAA AAGGAGAAAC AGCGCTTGGA ATACGAACGA 
TTCTTGAAGG AATATAGGGA GGACTGTAAA CGTCAGTTGC GTTTGCAGCG CGAGCATTAT 
CTGATCAAGA TTAAGCAGCT GGAGAAGGCC AGTCAGCGCT CGTTGAAGGG GAATCGAAAG 
CACAAGCTGA TGCAAAACGA TATGGTCAGC ATACCGGTGG CCAATTTGAA CGGCGGCCTT 
AAAGCGGCCA GCAGCGGTTC CGGAGTTGGT GTAGTGACCT CCGGCGGCGT CGTATCCTCG 
GCGGTGCTGG CCAATGCCCC ACGTGTATAC CTAACGCCCT CCTCCACATT CATGACCAAT 
CGCCAAATGG CGGGAGTGGC GTCCACCGGA AGAATGAGCG GACAAGTCGT CGGTGGTTCA 
TCTGGCACAG CCTCAACAGC CGGTACCGTT CGCTATTTTT CGCAATTCAG CAAAATGCAA 
ACGGCCGGCG GACCCAGTTT GCAAAGGAAA CTTGCCAACG GGGATACCAT TGTTCTGGCA 
ACCGGCAGTA AAAATATGTT CCTTACTAGC AGCGAAAATA AGGCTAATCT GCCGACCGTC 
GCCAGCAATG GCAATGGTCT GATCACAGCC AAAATGGATC TATTGGAGGA GGAGGTCATG 
CAGTCGATAA CTGTGATTGA TGACGATGAC GAGGAGAAAA AGGAAGTCGC TGAAGACGAA 
GAAGAGAGCA GCAATAATGC CAAACCCATT GACCTTCACC AGCCAATTGC TGATAATGAG 
CACGAGCTGG ACATTGTGAT AAACAATGTG GTCTGCTCGT TTAGCGTCGG CTGTCACCTG 
AAGCTGCGCG AAATTGCGCT GCAGGGATCA AATGTGGAGT ACCGTCGCGA GAACGGAATG 
GTGACAATGA AGCTCCGCCA TCCATACACC ACTGCCTCGA TTTGGTCGTC GGGGAGGATT 
ACCTGCACTG GTGCCACTTC CGAGTCAATG GCCAAGGTCG CTGCACGCCG CTATGCCAGA 
TGCCTTGGCA AGCTGGGATT TCCCACTCGT TTCCTCAACT TTCGCATTGT CAACGTACTG 
GGCACCTGCA GCATGCCGTG GGCCATCAAG ATCGTCAACT TTTCGGAGCG CCATCGCGAG 
AACGCCAGCT ACGAGCCCGA GCTGCATCCT GGAGTGACTT ACAAGATGCG CGATCCCGAT 
CCAAAGGCCA CCCTGAAGAT CTTCTCCACC GGCAGCGTAA CAGTCACCGC GGCTAGCGTA 
AACCACGTTG AATCGGCTAT CCAGCACATA TACCCGCTGG TCTTTGATTT CCGCAAGCAA 
CGTTCGGCGG AGGAGCTGCA GCATCTGCGT CAAAAGCAGC GTCTGCAAGC CGGCGGTGAT 
CCCCACGAAC TGGAGAAAAA TGTCTTGGCC GATAACAAGA CTGCTTCCCT TGATAATATC 
TTTGTTAATA CAACAGCGGC GCACTCTAAA TCATCGTCGA ATGACCAAAC ATCAGCACCC 
GCGACCATCT TGTCCAGCAC AGTTGATAGT ATGCCTCGTC TGAAACAGAT GGTCAACTAT 
CACCAGATGA TGAAGCAAAC CCAGGAAGAG CGCCGCCATA TAATGTTTAA TGGGGAGAAG 
GCCAATCCGG CCTCCACCTC ATCGGCGGCA GCAGCTCCCT CCACCTCCTC CTCATCGTCC 
TCATCCGGGG ATAATATCTG TGCCAATGCC CGTCGCCGGG CCACCGAATG CTGGGCAACC 
AAGCTGCAGA ATAAGCGTCC ACGTTACAAT GATCCCGGTA CCACGGGCAC AATTAACGCC 
GCATCGTCCA CAGCATCAGC CGCCACATCT TCGTTAGCCT CCCAGGCGAC ACATCTGCGT 
AATCCTCTCA AGACGGCCGC GTTGGCCAAT GCACGTATGT TAGGCGCCAA GGTGACAACA 
TGCACACGCA ACAGCATAAT CGTGCAGCAG CCGCAACGTA TCCAAATGCA GCAGCAGCAG 
CAACAGCTGC AACCGCAGCA GCAACAGACC AGCTTCTCGC CCAGCGAATT CGATGTTGAT 
GACTTGATCG AGGAGGAGGA GAACAACGAA TTGGATATGC CCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259330.1
CDS	673..5820
Translation	MGSAVKKGDGSPTRILEEYQVARDLRNEDLFPEHPVEHPDEDSDKENQSPNQSPNPRAIGDCEQGPDIGKDTLNPNEADQFSSEEVEKNEANSSCNESGGDARNSRDRLSSSHSEDEQLNQEYSTSSSGSTIGNREQGSDVGKDTSSRNEADQFSNEEVEKNEANSSGNESYGDACNSSDRLSSSNSEDEQLHQEYSTSSRGRTREYAQSDLNPGYQDLNIGNQDLNQVNREYQESNNEYQVSNQEYQHLNTEYQELNHGDQDSNDEYQELNHGNQDSNDEYQESNHGNQDSNDEYQELNHSNQDSNDEYQELNHGDQDSSDEYQDLNHVNQDSNHEYQAWNHEYQDSNHEYQALNREYQYPESDEEEAARKRNIYTRLRSYACQVHLPRLGESEVDSDDRRHNPINQPFNQPINRPNQKSDSSLESSSPERQDSESESRQASDEESSSGDSDYLIDPVTGWLSRRRETSTEREEKSDQSQQESSEEITDEQKEESDDVEQQSPAEHTSSDRRDHSPDKQQNKRKSLGTGNSSYSKKPLLDGDKGDPRSHLSSLGSRRSLKRKWPFDCTDSKKFKPNLQSEEDKSEEEKREDTESECSDEEDSDSSSFDTDSNSDSDSNLDLDSDSDSHSDFESSSSSEESAQDPAPEGETDQDPAPEGEADQDPAPEGGVDQDPAPEGGVDQDPAPEGEVDQDPAPEGESDQDLAPEGGVDQDPAPEFGTDQDPHPYYNDQDPAPYYDDQDPASYYDDHICDSEEDYKPFIFSKERKFLKFVTNPLGDDKSSLDLSIQLYKVFGAYKLEDVYSSIDEEGQPEQDLQLKQQQKHLKKYQKLQLVVFWHQEKFFSKEQLLLYEYLKLLLQKFFPQEQQEKRLQQEKLLQQEKLLQQEKRLQQEKRLQQEKRLQQEKRLQQEKRLQQQKLLQQQKLLQQEKLLQQQKLLQQEKLLQQEKLLQQEKQLQQEKHLPQERQLQQDKVFLQLRQLQQKKELEEQRERKRHLFEQQKQFVLEKLRQQKEQHRRQQNRVKRERKLRQKKEKQRLEYERFLKEYREDCKRQLRLQREHYLIKIKQLEKASQRSLKGNRKHKLMQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTNRQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLATGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDEEESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVLGTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASVNHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNIFVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEKANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINAASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQQQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF

Target ORF information:

RefSeq Version	NM_001272401.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272401.1

ORF Insert Sequence:

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAGTGAT 
CGCTTAAGTT CTTCTAATAG CGAGGACGAA CAGCTGCACC AAGAATATTC AACTTCATCA 
AGAGGAAGAA CAAGGGAATA TGCACAATCT GACTTGAATC CCGGGTACCA GGACTTGAAT 
ATCGGTAACC AGGACTTGAA TCAAGTTAAT CGCGAGTACC AGGAGTCTAA TAACGAGTAC 
CAGGTTTCAA ACCAAGAGTA CCAGCACTTG AATACCGAGT ACCAGGAGTT GAATCACGGC 
GATCAGGACT CAAATGACGA GTACCAGGAG TTGAATCACG GCAACCAGGA CTCAAATGAC 
GAGTACCAGG AATCAAATCA CGGCAACCAG GACTCAAATG ACGAGTACCA GGAGTTGAAT 
CACAGCAACC AGGACTCAAA TGACGAGTAC CAGGAGTTGA ATCACGGCGA TCAGGACTCA 
AGTGACGAGT ACCAGGATTT GAATCACGTC AACCAAGACT CAAATCACGA GTACCAGGCT 
TGGAATCACG AATACCAGGA CTCAAATCAC GAGTATCAGG CCTTGAATCG AGAGTACCAA 
TATCCTGAAT CTGATGAGGA AGAGGCCGCT AGAAAAAGAA ACATATATAC CCGTTTACGA 
TCCTACGCTT GTCAGGTACA TCTCCCTCGT TTAGGAGAAT CAGAAGTTGA TAGCGACGAT 
CGTCGCCATA ACCCAATCAA TCAGCCATTC AATCAGCCAA TCAATAGGCC AAATCAAAAA 
TCGGATTCAT CTTTAGAGTC ATCTTCGCCG GAGAGACAAG ACTCTGAAAG CGAGTCGCGA 
CAAGCTTCAG ATGAGGAATC GTCTTCTGGG GACTCGGACT ACTTAATCGA TCCAGTTACT 
GGGTGGCTAT CGCGCCGGCG AGAAACTTCT ACCGAACGCG AGGAAAAAAG CGACCAAAGC 
CAACAGGAGT CGTCAGAGGA AATTACAGAC GAACAGAAGG AAGAAAGCGA CGACGTCGAA 
CAACAGTCGC CGGCTGAACA TACTTCTTCT GATAGACGCG ATCATTCGCC AGACAAGCAA 
CAGAATAAGC GAAAATCGTT AGGCACTGGG AATTCTTCGT ATTCGAAGAA ACCATTATTG 
GACGGTGACA AAGGAGACCC ACGTTCACAT TTATCGTCTC TGGGAAGTCG CAGATCCTTA 
AAGCGTAAAT GGCCTTTTGA TTGTACAGAC TCTAAGAAAT TTAAACCAAA CTTGCAGAGT 
GAGGAAGACA AATCTGAGGA GGAAAAACGT GAAGATACGG AAAGCGAATG CTCTGACGAA 
GAAGACTCAG ATAGTTCAAG TTTCGATACA GATTCAAATT CAGATTCAGA TTCAAATTTA 
GATTTAGATT CCGATTCCGA TTCCCATTCC GATTTCGAAT CATCTTCAAG TTCTGAGGAA 
TCTGCCCAGG ATCCAGCTCC TGAAGGCGAA ACCGATCAAG ATCCAGCTCC TGAAGGCGAA 
GCCGATCAAG ATCCAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAAGGCGGA 
GTCGATCAAG ATCCAGCTCC TGAAGGCGAA GTCGATCAAG ATCCAGCTCC TGAAGGCGAA 
TCCGATCAAG ATCTAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAATTCGGA 
ACCGATCAAG ATCCACATCC TTATTACAAC GATCAGGATC CAGCTCCTTA TTACGACGAT 
CAGGATCCAG CTTCTTATTA CGACGATCAC ATTTGTGATT CTGAGGAGGA TTATAAACCA 
TTTATCTTCT CAAAGGAAAG GAAGTTCTTG AAATTCGTAA CAAATCCTTT AGGGGATGAT 
AAATCTAGTT TGGACCTTAG CATACAGCTC TATAAGGTAT TTGGAGCTTA CAAACTGGAA 
GACGTATACA GCAGTATTGA TGAGGAAGGC CAACCAGAAC AGGACCTGCA ACTGAAGCAG 
CAACAGAAAC ATTTAAAAAA ATATCAAAAG TTGCAACTGG TGGTATTCTG GCACCAGGAG 
AAATTCTTTT CAAAGGAGCA ACTGTTGCTA TATGAGTATC TGAAACTACT TTTGCAGAAA 
TTCTTTCCAC AAGAGCAACA GGAGAAACGG TTGCAACAGG AGAAACTTTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACGGTTGCAA CAGGAGAAAC GGTTGCAACA GGAGAAACGG 
TTGCAACAGG AGAAACGGTT GCAACAGGAG AAACGGTTGC AACAGCAGAA ACTCTTGCAA 
CAGCAGAAAC TCTTGCAACA GGAGAAACTC TTGCAACAGC AGAAACTCTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACTCTTGCAA CAGGAGAAAC AATTGCAACA GGAGAAACAC 
TTGCCACAGG AGCGACAGTT GCAACAGGAT AAAGTCTTCC TACAGCTACG ACAGTTGCAA 
CAGAAGAAAG AACTGGAGGA GCAGCGAGAG CGAAAGCGCC ACCTCTTTGA GCAACAGAAA 
CAGTTTGTCC TAGAAAAATT GCGCCAACAG AAGGAGCAGC ATCGTCGTCA GCAAAATCGC 
GTAAAACGCG AACGTAAATT GCGCCAGAAA AAGGAGAAAC AGCGCTTGGA ATACGAACGA 
TTCTTGAAGG AATATAGGGA GGACTGTAAA CGTCAGTTGC GTTTGCAGCG CGAGCATTAT 
CTGATCAAGA TTAAGCAGCT GGAGAAGGCC AGTCAGCGCT CGTTGAAGGG GAATCGAAAG 
CACAAGCTGA TGCAAAACGA TATGGTCAGC ATACCGGTGG CCAATTTGAA CGGCGGCCTT 
AAAGCGGCCA GCAGCGGTTC CGGAGTTGGT GTAGTGACCT CCGGCGGCGT CGTATCCTCG 
GCGGTGCTGG CCAATGCCCC ACGTGTATAC CTAACGCCCT CCTCCACATT CATGACCAAT 
CGCCAAATGG CGGGAGTGGC GTCCACCGGA AGAATGAGCG GACAAGTCGT CGGTGGTTCA 
TCTGGCACAG CCTCAACAGC CGGTACCGTT CGCTATTTTT CGCAATTCAG CAAAATGCAA 
ACGGCCGGCG GACCCAGTTT GCAAAGGAAA CTTGCCAACG GGGATACCAT TGTTCTGGCA 
ACCGGCAGTA AAAATATGTT CCTTACTAGC AGCGAAAATA AGGCTAATCT GCCGACCGTC 
GCCAGCAATG GCAATGGTCT GATCACAGCC AAAATGGATC TATTGGAGGA GGAGGTCATG 
CAGTCGATAA CTGTGATTGA TGACGATGAC GAGGAGAAAA AGGAAGTCGC TGAAGACGAA 
GAAGAGAGCA GCAATAATGC CAAACCCATT GACCTTCACC AGCCAATTGC TGATAATGAG 
CACGAGCTGG ACATTGTGAT AAACAATGTG GTCTGCTCGT TTAGCGTCGG CTGTCACCTG 
AAGCTGCGCG AAATTGCGCT GCAGGGATCA AATGTGGAGT ACCGTCGCGA GAACGGAATG 
GTGACAATGA AGCTCCGCCA TCCATACACC ACTGCCTCGA TTTGGTCGTC GGGGAGGATT 
ACCTGCACTG GTGCCACTTC CGAGTCAATG GCCAAGGTCG CTGCACGCCG CTATGCCAGA 
TGCCTTGGCA AGCTGGGATT TCCCACTCGT TTCCTCAACT TTCGCATTGT CAACGTACTG 
GGCACCTGCA GCATGCCGTG GGCCATCAAG ATCGTCAACT TTTCGGAGCG CCATCGCGAG 
AACGCCAGCT ACGAGCCCGA GCTGCATCCT GGAGTGACTT ACAAGATGCG CGATCCCGAT 
CCAAAGGCCA CCCTGAAGAT CTTCTCCACC GGCAGCGTAA CAGTCACCGC GGCTAGCGTA 
AACCACGTTG AATCGGCTAT CCAGCACATA TACCCGCTGG TCTTTGATTT CCGCAAGCAA 
CGTTCGGCGG AGGAGCTGCA GCATCTGCGT CAAAAGCAGC GTCTGCAAGC CGGCGGTGAT 
CCCCACGAAC TGGAGAAAAA TGTCTTGGCC GATAACAAGA CTGCTTCCCT TGATAATATC 
TTTGTTAATA CAACAGCGGC GCACTCTAAA TCATCGTCGA ATGACCAAAC ATCAGCACCC 
GCGACCATCT TGTCCAGCAC AGTTGATAGT ATGCCTCGTC TGAAACAGAT GGTCAACTAT 
CACCAGATGA TGAAGCAAAC CCAGGAAGAG CGCCGCCATA TAATGTTTAA TGGGGAGAAG 
GCCAATCCGG CCTCCACCTC ATCGGCGGCA GCAGCTCCCT CCACCTCCTC CTCATCGTCC 
TCATCCGGGG ATAATATCTG TGCCAATGCC CGTCGCCGGG CCACCGAATG CTGGGCAACC 
AAGCTGCAGA ATAAGCGTCC ACGTTACAAT GATCCCGGTA CCACGGGCAC AATTAACGCC 
GCATCGTCCA CAGCATCAGC CGCCACATCT TCGTTAGCCT CCCAGGCGAC ACATCTGCGT 
AATCCTCTCA AGACGGCCGC GTTGGCCAAT GCACGTATGT TAGGCGCCAA GGTGACAACA 
TGCACACGCA ACAGCATAAT CGTGCAGCAG CCGCAACGTA TCCAAATGCA GCAGCAGCAG 
CAACAGCTGC AACCGCAGCA GCAACAGACC AGCTTCTCGC CCAGCGAATT CGATGTTGAT 
GACTTGATCG AGGAGGAGGA GAACAACGAA TTGGATATGC CCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf2 ( NM_001103435.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096905.1 Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant E, mRNA.

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CloneID	OFa01111
Clone ID Related Accession (Same CDS sequence)	NM_001272401.1 , NM_001103435.3 , NM_001169230.2
Accession Version	NM_001103435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TATA box binding protein-related factor 2, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103435.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAGTGAT 
CGCTTAAGTT CTTCTAATAG CGAGGACGAA CAGCTGCACC AAGAATATTC AACTTCATCA 
AGAGGAAGAA CAAGGGAATA TGCACAATCT GACTTGAATC CCGGGTACCA GGACTTGAAT 
ATCGGTAACC AGGACTTGAA TCAAGTTAAT CGCGAGTACC AGGAGTCTAA TAACGAGTAC 
CAGGTTTCAA ACCAAGAGTA CCAGCACTTG AATACCGAGT ACCAGGAGTT GAATCACGGC 
GATCAGGACT CAAATGACGA GTACCAGGAG TTGAATCACG GCAACCAGGA CTCAAATGAC 
GAGTACCAGG AATCAAATCA CGGCAACCAG GACTCAAATG ACGAGTACCA GGAGTTGAAT 
CACAGCAACC AGGACTCAAA TGACGAGTAC CAGGAGTTGA ATCACGGCGA TCAGGACTCA 
AGTGACGAGT ACCAGGATTT GAATCACGTC AACCAAGACT CAAATCACGA GTACCAGGCT 
TGGAATCACG AATACCAGGA CTCAAATCAC GAGTATCAGG CCTTGAATCG AGAGTACCAA 
TATCCTGAAT CTGATGAGGA AGAGGCCGCT AGAAAAAGAA ACATATATAC CCGTTTACGA 
TCCTACGCTT GTCAGGTACA TCTCCCTCGT TTAGGAGAAT CAGAAGTTGA TAGCGACGAT 
CGTCGCCATA ACCCAATCAA TCAGCCATTC AATCAGCCAA TCAATAGGCC AAATCAAAAA 
TCGGATTCAT CTTTAGAGTC ATCTTCGCCG GAGAGACAAG ACTCTGAAAG CGAGTCGCGA 
CAAGCTTCAG ATGAGGAATC GTCTTCTGGG GACTCGGACT ACTTAATCGA TCCAGTTACT 
GGGTGGCTAT CGCGCCGGCG AGAAACTTCT ACCGAACGCG AGGAAAAAAG CGACCAAAGC 
CAACAGGAGT CGTCAGAGGA AATTACAGAC GAACAGAAGG AAGAAAGCGA CGACGTCGAA 
CAACAGTCGC CGGCTGAACA TACTTCTTCT GATAGACGCG ATCATTCGCC AGACAAGCAA 
CAGAATAAGC GAAAATCGTT AGGCACTGGG AATTCTTCGT ATTCGAAGAA ACCATTATTG 
GACGGTGACA AAGGAGACCC ACGTTCACAT TTATCGTCTC TGGGAAGTCG CAGATCCTTA 
AAGCGTAAAT GGCCTTTTGA TTGTACAGAC TCTAAGAAAT TTAAACCAAA CTTGCAGAGT 
GAGGAAGACA AATCTGAGGA GGAAAAACGT GAAGATACGG AAAGCGAATG CTCTGACGAA 
GAAGACTCAG ATAGTTCAAG TTTCGATACA GATTCAAATT CAGATTCAGA TTCAAATTTA 
GATTTAGATT CCGATTCCGA TTCCCATTCC GATTTCGAAT CATCTTCAAG TTCTGAGGAA 
TCTGCCCAGG ATCCAGCTCC TGAAGGCGAA ACCGATCAAG ATCCAGCTCC TGAAGGCGAA 
GCCGATCAAG ATCCAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAAGGCGGA 
GTCGATCAAG ATCCAGCTCC TGAAGGCGAA GTCGATCAAG ATCCAGCTCC TGAAGGCGAA 
TCCGATCAAG ATCTAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAATTCGGA 
ACCGATCAAG ATCCACATCC TTATTACAAC GATCAGGATC CAGCTCCTTA TTACGACGAT 
CAGGATCCAG CTTCTTATTA CGACGATCAC ATTTGTGATT CTGAGGAGGA TTATAAACCA 
TTTATCTTCT CAAAGGAAAG GAAGTTCTTG AAATTCGTAA CAAATCCTTT AGGGGATGAT 
AAATCTAGTT TGGACCTTAG CATACAGCTC TATAAGGTAT TTGGAGCTTA CAAACTGGAA 
GACGTATACA GCAGTATTGA TGAGGAAGGC CAACCAGAAC AGGACCTGCA ACTGAAGCAG 
CAACAGAAAC ATTTAAAAAA ATATCAAAAG TTGCAACTGG TGGTATTCTG GCACCAGGAG 
AAATTCTTTT CAAAGGAGCA ACTGTTGCTA TATGAGTATC TGAAACTACT TTTGCAGAAA 
TTCTTTCCAC AAGAGCAACA GGAGAAACGG TTGCAACAGG AGAAACTTTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACGGTTGCAA CAGGAGAAAC GGTTGCAACA GGAGAAACGG 
TTGCAACAGG AGAAACGGTT GCAACAGGAG AAACGGTTGC AACAGCAGAA ACTCTTGCAA 
CAGCAGAAAC TCTTGCAACA GGAGAAACTC TTGCAACAGC AGAAACTCTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACTCTTGCAA CAGGAGAAAC AATTGCAACA GGAGAAACAC 
TTGCCACAGG AGCGACAGTT GCAACAGGAT AAAGTCTTCC TACAGCTACG ACAGTTGCAA 
CAGAAGAAAG AACTGGAGGA GCAGCGAGAG CGAAAGCGCC ACCTCTTTGA GCAACAGAAA 
CAGTTTGTCC TAGAAAAATT GCGCCAACAG AAGGAGCAGC ATCGTCGTCA GCAAAATCGC 
GTAAAACGCG AACGTAAATT GCGCCAGAAA AAGGAGAAAC AGCGCTTGGA ATACGAACGA 
TTCTTGAAGG AATATAGGGA GGACTGTAAA CGTCAGTTGC GTTTGCAGCG CGAGCATTAT 
CTGATCAAGA TTAAGCAGCT GGAGAAGGCC AGTCAGCGCT CGTTGAAGGG GAATCGAAAG 
CACAAGCTGA TGCAAAACGA TATGGTCAGC ATACCGGTGG CCAATTTGAA CGGCGGCCTT 
AAAGCGGCCA GCAGCGGTTC CGGAGTTGGT GTAGTGACCT CCGGCGGCGT CGTATCCTCG 
GCGGTGCTGG CCAATGCCCC ACGTGTATAC CTAACGCCCT CCTCCACATT CATGACCAAT 
CGCCAAATGG CGGGAGTGGC GTCCACCGGA AGAATGAGCG GACAAGTCGT CGGTGGTTCA 
TCTGGCACAG CCTCAACAGC CGGTACCGTT CGCTATTTTT CGCAATTCAG CAAAATGCAA 
ACGGCCGGCG GACCCAGTTT GCAAAGGAAA CTTGCCAACG GGGATACCAT TGTTCTGGCA 
ACCGGCAGTA AAAATATGTT CCTTACTAGC AGCGAAAATA AGGCTAATCT GCCGACCGTC 
GCCAGCAATG GCAATGGTCT GATCACAGCC AAAATGGATC TATTGGAGGA GGAGGTCATG 
CAGTCGATAA CTGTGATTGA TGACGATGAC GAGGAGAAAA AGGAAGTCGC TGAAGACGAA 
GAAGAGAGCA GCAATAATGC CAAACCCATT GACCTTCACC AGCCAATTGC TGATAATGAG 
CACGAGCTGG ACATTGTGAT AAACAATGTG GTCTGCTCGT TTAGCGTCGG CTGTCACCTG 
AAGCTGCGCG AAATTGCGCT GCAGGGATCA AATGTGGAGT ACCGTCGCGA GAACGGAATG 
GTGACAATGA AGCTCCGCCA TCCATACACC ACTGCCTCGA TTTGGTCGTC GGGGAGGATT 
ACCTGCACTG GTGCCACTTC CGAGTCAATG GCCAAGGTCG CTGCACGCCG CTATGCCAGA 
TGCCTTGGCA AGCTGGGATT TCCCACTCGT TTCCTCAACT TTCGCATTGT CAACGTACTG 
GGCACCTGCA GCATGCCGTG GGCCATCAAG ATCGTCAACT TTTCGGAGCG CCATCGCGAG 
AACGCCAGCT ACGAGCCCGA GCTGCATCCT GGAGTGACTT ACAAGATGCG CGATCCCGAT 
CCAAAGGCCA CCCTGAAGAT CTTCTCCACC GGCAGCGTAA CAGTCACCGC GGCTAGCGTA 
AACCACGTTG AATCGGCTAT CCAGCACATA TACCCGCTGG TCTTTGATTT CCGCAAGCAA 
CGTTCGGCGG AGGAGCTGCA GCATCTGCGT CAAAAGCAGC GTCTGCAAGC CGGCGGTGAT 
CCCCACGAAC TGGAGAAAAA TGTCTTGGCC GATAACAAGA CTGCTTCCCT TGATAATATC 
TTTGTTAATA CAACAGCGGC GCACTCTAAA TCATCGTCGA ATGACCAAAC ATCAGCACCC 
GCGACCATCT TGTCCAGCAC AGTTGATAGT ATGCCTCGTC TGAAACAGAT GGTCAACTAT 
CACCAGATGA TGAAGCAAAC CCAGGAAGAG CGCCGCCATA TAATGTTTAA TGGGGAGAAG 
GCCAATCCGG CCTCCACCTC ATCGGCGGCA GCAGCTCCCT CCACCTCCTC CTCATCGTCC 
TCATCCGGGG ATAATATCTG TGCCAATGCC CGTCGCCGGG CCACCGAATG CTGGGCAACC 
AAGCTGCAGA ATAAGCGTCC ACGTTACAAT GATCCCGGTA CCACGGGCAC AATTAACGCC 
GCATCGTCCA CAGCATCAGC CGCCACATCT TCGTTAGCCT CCCAGGCGAC ACATCTGCGT 
AATCCTCTCA AGACGGCCGC GTTGGCCAAT GCACGTATGT TAGGCGCCAA GGTGACAACA 
TGCACACGCA ACAGCATAAT CGTGCAGCAG CCGCAACGTA TCCAAATGCA GCAGCAGCAG 
CAACAGCTGC AACCGCAGCA GCAACAGACC AGCTTCTCGC CCAGCGAATT CGATGTTGAT 
GACTTGATCG AGGAGGAGGA GAACAACGAA TTGGATATGC CCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096905.1
CDS	964..6111
Translation	MGSAVKKGDGSPTRILEEYQVARDLRNEDLFPEHPVEHPDEDSDKENQSPNQSPNPRAIGDCEQGPDIGKDTLNPNEADQFSSEEVEKNEANSSCNESGGDARNSRDRLSSSHSEDEQLNQEYSTSSSGSTIGNREQGSDVGKDTSSRNEADQFSNEEVEKNEANSSGNESYGDACNSSDRLSSSNSEDEQLHQEYSTSSRGRTREYAQSDLNPGYQDLNIGNQDLNQVNREYQESNNEYQVSNQEYQHLNTEYQELNHGDQDSNDEYQELNHGNQDSNDEYQESNHGNQDSNDEYQELNHSNQDSNDEYQELNHGDQDSSDEYQDLNHVNQDSNHEYQAWNHEYQDSNHEYQALNREYQYPESDEEEAARKRNIYTRLRSYACQVHLPRLGESEVDSDDRRHNPINQPFNQPINRPNQKSDSSLESSSPERQDSESESRQASDEESSSGDSDYLIDPVTGWLSRRRETSTEREEKSDQSQQESSEEITDEQKEESDDVEQQSPAEHTSSDRRDHSPDKQQNKRKSLGTGNSSYSKKPLLDGDKGDPRSHLSSLGSRRSLKRKWPFDCTDSKKFKPNLQSEEDKSEEEKREDTESECSDEEDSDSSSFDTDSNSDSDSNLDLDSDSDSHSDFESSSSSEESAQDPAPEGETDQDPAPEGEADQDPAPEGGVDQDPAPEGGVDQDPAPEGEVDQDPAPEGESDQDLAPEGGVDQDPAPEFGTDQDPHPYYNDQDPAPYYDDQDPASYYDDHICDSEEDYKPFIFSKERKFLKFVTNPLGDDKSSLDLSIQLYKVFGAYKLEDVYSSIDEEGQPEQDLQLKQQQKHLKKYQKLQLVVFWHQEKFFSKEQLLLYEYLKLLLQKFFPQEQQEKRLQQEKLLQQEKLLQQEKRLQQEKRLQQEKRLQQEKRLQQEKRLQQQKLLQQQKLLQQEKLLQQQKLLQQEKLLQQEKLLQQEKQLQQEKHLPQERQLQQDKVFLQLRQLQQKKELEEQRERKRHLFEQQKQFVLEKLRQQKEQHRRQQNRVKRERKLRQKKEKQRLEYERFLKEYREDCKRQLRLQREHYLIKIKQLEKASQRSLKGNRKHKLMQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTNRQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLATGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDEEESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVLGTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASVNHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNIFVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEKANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINAASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQQQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF

Target ORF information:

RefSeq Version	NM_001103435.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103435.3

ORF Insert Sequence:

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAGTGAT 
CGCTTAAGTT CTTCTAATAG CGAGGACGAA CAGCTGCACC AAGAATATTC AACTTCATCA 
AGAGGAAGAA CAAGGGAATA TGCACAATCT GACTTGAATC CCGGGTACCA GGACTTGAAT 
ATCGGTAACC AGGACTTGAA TCAAGTTAAT CGCGAGTACC AGGAGTCTAA TAACGAGTAC 
CAGGTTTCAA ACCAAGAGTA CCAGCACTTG AATACCGAGT ACCAGGAGTT GAATCACGGC 
GATCAGGACT CAAATGACGA GTACCAGGAG TTGAATCACG GCAACCAGGA CTCAAATGAC 
GAGTACCAGG AATCAAATCA CGGCAACCAG GACTCAAATG ACGAGTACCA GGAGTTGAAT 
CACAGCAACC AGGACTCAAA TGACGAGTAC CAGGAGTTGA ATCACGGCGA TCAGGACTCA 
AGTGACGAGT ACCAGGATTT GAATCACGTC AACCAAGACT CAAATCACGA GTACCAGGCT 
TGGAATCACG AATACCAGGA CTCAAATCAC GAGTATCAGG CCTTGAATCG AGAGTACCAA 
TATCCTGAAT CTGATGAGGA AGAGGCCGCT AGAAAAAGAA ACATATATAC CCGTTTACGA 
TCCTACGCTT GTCAGGTACA TCTCCCTCGT TTAGGAGAAT CAGAAGTTGA TAGCGACGAT 
CGTCGCCATA ACCCAATCAA TCAGCCATTC AATCAGCCAA TCAATAGGCC AAATCAAAAA 
TCGGATTCAT CTTTAGAGTC ATCTTCGCCG GAGAGACAAG ACTCTGAAAG CGAGTCGCGA 
CAAGCTTCAG ATGAGGAATC GTCTTCTGGG GACTCGGACT ACTTAATCGA TCCAGTTACT 
GGGTGGCTAT CGCGCCGGCG AGAAACTTCT ACCGAACGCG AGGAAAAAAG CGACCAAAGC 
CAACAGGAGT CGTCAGAGGA AATTACAGAC GAACAGAAGG AAGAAAGCGA CGACGTCGAA 
CAACAGTCGC CGGCTGAACA TACTTCTTCT GATAGACGCG ATCATTCGCC AGACAAGCAA 
CAGAATAAGC GAAAATCGTT AGGCACTGGG AATTCTTCGT ATTCGAAGAA ACCATTATTG 
GACGGTGACA AAGGAGACCC ACGTTCACAT TTATCGTCTC TGGGAAGTCG CAGATCCTTA 
AAGCGTAAAT GGCCTTTTGA TTGTACAGAC TCTAAGAAAT TTAAACCAAA CTTGCAGAGT 
GAGGAAGACA AATCTGAGGA GGAAAAACGT GAAGATACGG AAAGCGAATG CTCTGACGAA 
GAAGACTCAG ATAGTTCAAG TTTCGATACA GATTCAAATT CAGATTCAGA TTCAAATTTA 
GATTTAGATT CCGATTCCGA TTCCCATTCC GATTTCGAAT CATCTTCAAG TTCTGAGGAA 
TCTGCCCAGG ATCCAGCTCC TGAAGGCGAA ACCGATCAAG ATCCAGCTCC TGAAGGCGAA 
GCCGATCAAG ATCCAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAAGGCGGA 
GTCGATCAAG ATCCAGCTCC TGAAGGCGAA GTCGATCAAG ATCCAGCTCC TGAAGGCGAA 
TCCGATCAAG ATCTAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAATTCGGA 
ACCGATCAAG ATCCACATCC TTATTACAAC GATCAGGATC CAGCTCCTTA TTACGACGAT 
CAGGATCCAG CTTCTTATTA CGACGATCAC ATTTGTGATT CTGAGGAGGA TTATAAACCA 
TTTATCTTCT CAAAGGAAAG GAAGTTCTTG AAATTCGTAA CAAATCCTTT AGGGGATGAT 
AAATCTAGTT TGGACCTTAG CATACAGCTC TATAAGGTAT TTGGAGCTTA CAAACTGGAA 
GACGTATACA GCAGTATTGA TGAGGAAGGC CAACCAGAAC AGGACCTGCA ACTGAAGCAG 
CAACAGAAAC ATTTAAAAAA ATATCAAAAG TTGCAACTGG TGGTATTCTG GCACCAGGAG 
AAATTCTTTT CAAAGGAGCA ACTGTTGCTA TATGAGTATC TGAAACTACT TTTGCAGAAA 
TTCTTTCCAC AAGAGCAACA GGAGAAACGG TTGCAACAGG AGAAACTTTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACGGTTGCAA CAGGAGAAAC GGTTGCAACA GGAGAAACGG 
TTGCAACAGG AGAAACGGTT GCAACAGGAG AAACGGTTGC AACAGCAGAA ACTCTTGCAA 
CAGCAGAAAC TCTTGCAACA GGAGAAACTC TTGCAACAGC AGAAACTCTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACTCTTGCAA CAGGAGAAAC AATTGCAACA GGAGAAACAC 
TTGCCACAGG AGCGACAGTT GCAACAGGAT AAAGTCTTCC TACAGCTACG ACAGTTGCAA 
CAGAAGAAAG AACTGGAGGA GCAGCGAGAG CGAAAGCGCC ACCTCTTTGA GCAACAGAAA 
CAGTTTGTCC TAGAAAAATT GCGCCAACAG AAGGAGCAGC ATCGTCGTCA GCAAAATCGC 
GTAAAACGCG AACGTAAATT GCGCCAGAAA AAGGAGAAAC AGCGCTTGGA ATACGAACGA 
TTCTTGAAGG AATATAGGGA GGACTGTAAA CGTCAGTTGC GTTTGCAGCG CGAGCATTAT 
CTGATCAAGA TTAAGCAGCT GGAGAAGGCC AGTCAGCGCT CGTTGAAGGG GAATCGAAAG 
CACAAGCTGA TGCAAAACGA TATGGTCAGC ATACCGGTGG CCAATTTGAA CGGCGGCCTT 
AAAGCGGCCA GCAGCGGTTC CGGAGTTGGT GTAGTGACCT CCGGCGGCGT CGTATCCTCG 
GCGGTGCTGG CCAATGCCCC ACGTGTATAC CTAACGCCCT CCTCCACATT CATGACCAAT 
CGCCAAATGG CGGGAGTGGC GTCCACCGGA AGAATGAGCG GACAAGTCGT CGGTGGTTCA 
TCTGGCACAG CCTCAACAGC CGGTACCGTT CGCTATTTTT CGCAATTCAG CAAAATGCAA 
ACGGCCGGCG GACCCAGTTT GCAAAGGAAA CTTGCCAACG GGGATACCAT TGTTCTGGCA 
ACCGGCAGTA AAAATATGTT CCTTACTAGC AGCGAAAATA AGGCTAATCT GCCGACCGTC 
GCCAGCAATG GCAATGGTCT GATCACAGCC AAAATGGATC TATTGGAGGA GGAGGTCATG 
CAGTCGATAA CTGTGATTGA TGACGATGAC GAGGAGAAAA AGGAAGTCGC TGAAGACGAA 
GAAGAGAGCA GCAATAATGC CAAACCCATT GACCTTCACC AGCCAATTGC TGATAATGAG 
CACGAGCTGG ACATTGTGAT AAACAATGTG GTCTGCTCGT TTAGCGTCGG CTGTCACCTG 
AAGCTGCGCG AAATTGCGCT GCAGGGATCA AATGTGGAGT ACCGTCGCGA GAACGGAATG 
GTGACAATGA AGCTCCGCCA TCCATACACC ACTGCCTCGA TTTGGTCGTC GGGGAGGATT 
ACCTGCACTG GTGCCACTTC CGAGTCAATG GCCAAGGTCG CTGCACGCCG CTATGCCAGA 
TGCCTTGGCA AGCTGGGATT TCCCACTCGT TTCCTCAACT TTCGCATTGT CAACGTACTG 
GGCACCTGCA GCATGCCGTG GGCCATCAAG ATCGTCAACT TTTCGGAGCG CCATCGCGAG 
AACGCCAGCT ACGAGCCCGA GCTGCATCCT GGAGTGACTT ACAAGATGCG CGATCCCGAT 
CCAAAGGCCA CCCTGAAGAT CTTCTCCACC GGCAGCGTAA CAGTCACCGC GGCTAGCGTA 
AACCACGTTG AATCGGCTAT CCAGCACATA TACCCGCTGG TCTTTGATTT CCGCAAGCAA 
CGTTCGGCGG AGGAGCTGCA GCATCTGCGT CAAAAGCAGC GTCTGCAAGC CGGCGGTGAT 
CCCCACGAAC TGGAGAAAAA TGTCTTGGCC GATAACAAGA CTGCTTCCCT TGATAATATC 
TTTGTTAATA CAACAGCGGC GCACTCTAAA TCATCGTCGA ATGACCAAAC ATCAGCACCC 
GCGACCATCT TGTCCAGCAC AGTTGATAGT ATGCCTCGTC TGAAACAGAT GGTCAACTAT 
CACCAGATGA TGAAGCAAAC CCAGGAAGAG CGCCGCCATA TAATGTTTAA TGGGGAGAAG 
GCCAATCCGG CCTCCACCTC ATCGGCGGCA GCAGCTCCCT CCACCTCCTC CTCATCGTCC 
TCATCCGGGG ATAATATCTG TGCCAATGCC CGTCGCCGGG CCACCGAATG CTGGGCAACC 
AAGCTGCAGA ATAAGCGTCC ACGTTACAAT GATCCCGGTA CCACGGGCAC AATTAACGCC 
GCATCGTCCA CAGCATCAGC CGCCACATCT TCGTTAGCCT CCCAGGCGAC ACATCTGCGT 
AATCCTCTCA AGACGGCCGC GTTGGCCAAT GCACGTATGT TAGGCGCCAA GGTGACAACA 
TGCACACGCA ACAGCATAAT CGTGCAGCAG CCGCAACGTA TCCAAATGCA GCAGCAGCAG 
CAACAGCTGC AACCGCAGCA GCAACAGACC AGCTTCTCGC CCAGCGAATT CGATGTTGAT 
GACTTGATCG AGGAGGAGGA GAACAACGAA TTGGATATGC CCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf2 ( NM_001169230.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162701.1 Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant F, mRNA.

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CloneID	OFa01111
Clone ID Related Accession (Same CDS sequence)	NM_001272401.1 , NM_001103435.3 , NM_001169230.2
Accession Version	NM_001169230.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TATA box binding protein-related factor 2, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169230.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAGTGAT 
CGCTTAAGTT CTTCTAATAG CGAGGACGAA CAGCTGCACC AAGAATATTC AACTTCATCA 
AGAGGAAGAA CAAGGGAATA TGCACAATCT GACTTGAATC CCGGGTACCA GGACTTGAAT 
ATCGGTAACC AGGACTTGAA TCAAGTTAAT CGCGAGTACC AGGAGTCTAA TAACGAGTAC 
CAGGTTTCAA ACCAAGAGTA CCAGCACTTG AATACCGAGT ACCAGGAGTT GAATCACGGC 
GATCAGGACT CAAATGACGA GTACCAGGAG TTGAATCACG GCAACCAGGA CTCAAATGAC 
GAGTACCAGG AATCAAATCA CGGCAACCAG GACTCAAATG ACGAGTACCA GGAGTTGAAT 
CACAGCAACC AGGACTCAAA TGACGAGTAC CAGGAGTTGA ATCACGGCGA TCAGGACTCA 
AGTGACGAGT ACCAGGATTT GAATCACGTC AACCAAGACT CAAATCACGA GTACCAGGCT 
TGGAATCACG AATACCAGGA CTCAAATCAC GAGTATCAGG CCTTGAATCG AGAGTACCAA 
TATCCTGAAT CTGATGAGGA AGAGGCCGCT AGAAAAAGAA ACATATATAC CCGTTTACGA 
TCCTACGCTT GTCAGGTACA TCTCCCTCGT TTAGGAGAAT CAGAAGTTGA TAGCGACGAT 
CGTCGCCATA ACCCAATCAA TCAGCCATTC AATCAGCCAA TCAATAGGCC AAATCAAAAA 
TCGGATTCAT CTTTAGAGTC ATCTTCGCCG GAGAGACAAG ACTCTGAAAG CGAGTCGCGA 
CAAGCTTCAG ATGAGGAATC GTCTTCTGGG GACTCGGACT ACTTAATCGA TCCAGTTACT 
GGGTGGCTAT CGCGCCGGCG AGAAACTTCT ACCGAACGCG AGGAAAAAAG CGACCAAAGC 
CAACAGGAGT CGTCAGAGGA AATTACAGAC GAACAGAAGG AAGAAAGCGA CGACGTCGAA 
CAACAGTCGC CGGCTGAACA TACTTCTTCT GATAGACGCG ATCATTCGCC AGACAAGCAA 
CAGAATAAGC GAAAATCGTT AGGCACTGGG AATTCTTCGT ATTCGAAGAA ACCATTATTG 
GACGGTGACA AAGGAGACCC ACGTTCACAT TTATCGTCTC TGGGAAGTCG CAGATCCTTA 
AAGCGTAAAT GGCCTTTTGA TTGTACAGAC TCTAAGAAAT TTAAACCAAA CTTGCAGAGT 
GAGGAAGACA AATCTGAGGA GGAAAAACGT GAAGATACGG AAAGCGAATG CTCTGACGAA 
GAAGACTCAG ATAGTTCAAG TTTCGATACA GATTCAAATT CAGATTCAGA TTCAAATTTA 
GATTTAGATT CCGATTCCGA TTCCCATTCC GATTTCGAAT CATCTTCAAG TTCTGAGGAA 
TCTGCCCAGG ATCCAGCTCC TGAAGGCGAA ACCGATCAAG ATCCAGCTCC TGAAGGCGAA 
GCCGATCAAG ATCCAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAAGGCGGA 
GTCGATCAAG ATCCAGCTCC TGAAGGCGAA GTCGATCAAG ATCCAGCTCC TGAAGGCGAA 
TCCGATCAAG ATCTAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAATTCGGA 
ACCGATCAAG ATCCACATCC TTATTACAAC GATCAGGATC CAGCTCCTTA TTACGACGAT 
CAGGATCCAG CTTCTTATTA CGACGATCAC ATTTGTGATT CTGAGGAGGA TTATAAACCA 
TTTATCTTCT CAAAGGAAAG GAAGTTCTTG AAATTCGTAA CAAATCCTTT AGGGGATGAT 
AAATCTAGTT TGGACCTTAG CATACAGCTC TATAAGGTAT TTGGAGCTTA CAAACTGGAA 
GACGTATACA GCAGTATTGA TGAGGAAGGC CAACCAGAAC AGGACCTGCA ACTGAAGCAG 
CAACAGAAAC ATTTAAAAAA ATATCAAAAG TTGCAACTGG TGGTATTCTG GCACCAGGAG 
AAATTCTTTT CAAAGGAGCA ACTGTTGCTA TATGAGTATC TGAAACTACT TTTGCAGAAA 
TTCTTTCCAC AAGAGCAACA GGAGAAACGG TTGCAACAGG AGAAACTTTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACGGTTGCAA CAGGAGAAAC GGTTGCAACA GGAGAAACGG 
TTGCAACAGG AGAAACGGTT GCAACAGGAG AAACGGTTGC AACAGCAGAA ACTCTTGCAA 
CAGCAGAAAC TCTTGCAACA GGAGAAACTC TTGCAACAGC AGAAACTCTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACTCTTGCAA CAGGAGAAAC AATTGCAACA GGAGAAACAC 
TTGCCACAGG AGCGACAGTT GCAACAGGAT AAAGTCTTCC TACAGCTACG ACAGTTGCAA 
CAGAAGAAAG AACTGGAGGA GCAGCGAGAG CGAAAGCGCC ACCTCTTTGA GCAACAGAAA 
CAGTTTGTCC TAGAAAAATT GCGCCAACAG AAGGAGCAGC ATCGTCGTCA GCAAAATCGC 
GTAAAACGCG AACGTAAATT GCGCCAGAAA AAGGAGAAAC AGCGCTTGGA ATACGAACGA 
TTCTTGAAGG AATATAGGGA GGACTGTAAA CGTCAGTTGC GTTTGCAGCG CGAGCATTAT 
CTGATCAAGA TTAAGCAGCT GGAGAAGGCC AGTCAGCGCT CGTTGAAGGG GAATCGAAAG 
CACAAGCTGA TGCAAAACGA TATGGTCAGC ATACCGGTGG CCAATTTGAA CGGCGGCCTT 
AAAGCGGCCA GCAGCGGTTC CGGAGTTGGT GTAGTGACCT CCGGCGGCGT CGTATCCTCG 
GCGGTGCTGG CCAATGCCCC ACGTGTATAC CTAACGCCCT CCTCCACATT CATGACCAAT 
CGCCAAATGG CGGGAGTGGC GTCCACCGGA AGAATGAGCG GACAAGTCGT CGGTGGTTCA 
TCTGGCACAG CCTCAACAGC CGGTACCGTT CGCTATTTTT CGCAATTCAG CAAAATGCAA 
ACGGCCGGCG GACCCAGTTT GCAAAGGAAA CTTGCCAACG GGGATACCAT TGTTCTGGCA 
ACCGGCAGTA AAAATATGTT CCTTACTAGC AGCGAAAATA AGGCTAATCT GCCGACCGTC 
GCCAGCAATG GCAATGGTCT GATCACAGCC AAAATGGATC TATTGGAGGA GGAGGTCATG 
CAGTCGATAA CTGTGATTGA TGACGATGAC GAGGAGAAAA AGGAAGTCGC TGAAGACGAA 
GAAGAGAGCA GCAATAATGC CAAACCCATT GACCTTCACC AGCCAATTGC TGATAATGAG 
CACGAGCTGG ACATTGTGAT AAACAATGTG GTCTGCTCGT TTAGCGTCGG CTGTCACCTG 
AAGCTGCGCG AAATTGCGCT GCAGGGATCA AATGTGGAGT ACCGTCGCGA GAACGGAATG 
GTGACAATGA AGCTCCGCCA TCCATACACC ACTGCCTCGA TTTGGTCGTC GGGGAGGATT 
ACCTGCACTG GTGCCACTTC CGAGTCAATG GCCAAGGTCG CTGCACGCCG CTATGCCAGA 
TGCCTTGGCA AGCTGGGATT TCCCACTCGT TTCCTCAACT TTCGCATTGT CAACGTACTG 
GGCACCTGCA GCATGCCGTG GGCCATCAAG ATCGTCAACT TTTCGGAGCG CCATCGCGAG 
AACGCCAGCT ACGAGCCCGA GCTGCATCCT GGAGTGACTT ACAAGATGCG CGATCCCGAT 
CCAAAGGCCA CCCTGAAGAT CTTCTCCACC GGCAGCGTAA CAGTCACCGC GGCTAGCGTA 
AACCACGTTG AATCGGCTAT CCAGCACATA TACCCGCTGG TCTTTGATTT CCGCAAGCAA 
CGTTCGGCGG AGGAGCTGCA GCATCTGCGT CAAAAGCAGC GTCTGCAAGC CGGCGGTGAT 
CCCCACGAAC TGGAGAAAAA TGTCTTGGCC GATAACAAGA CTGCTTCCCT TGATAATATC 
TTTGTTAATA CAACAGCGGC GCACTCTAAA TCATCGTCGA ATGACCAAAC ATCAGCACCC 
GCGACCATCT TGTCCAGCAC AGTTGATAGT ATGCCTCGTC TGAAACAGAT GGTCAACTAT 
CACCAGATGA TGAAGCAAAC CCAGGAAGAG CGCCGCCATA TAATGTTTAA TGGGGAGAAG 
GCCAATCCGG CCTCCACCTC ATCGGCGGCA GCAGCTCCCT CCACCTCCTC CTCATCGTCC 
TCATCCGGGG ATAATATCTG TGCCAATGCC CGTCGCCGGG CCACCGAATG CTGGGCAACC 
AAGCTGCAGA ATAAGCGTCC ACGTTACAAT GATCCCGGTA CCACGGGCAC AATTAACGCC 
GCATCGTCCA CAGCATCAGC CGCCACATCT TCGTTAGCCT CCCAGGCGAC ACATCTGCGT 
AATCCTCTCA AGACGGCCGC GTTGGCCAAT GCACGTATGT TAGGCGCCAA GGTGACAACA 
TGCACACGCA ACAGCATAAT CGTGCAGCAG CCGCAACGTA TCCAAATGCA GCAGCAGCAG 
CAACAGCTGC AACCGCAGCA GCAACAGACC AGCTTCTCGC CCAGCGAATT CGATGTTGAT 
GACTTGATCG AGGAGGAGGA GAACAACGAA TTGGATATGC CCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162701.1
CDS	883..6030
Translation	MGSAVKKGDGSPTRILEEYQVARDLRNEDLFPEHPVEHPDEDSDKENQSPNQSPNPRAIGDCEQGPDIGKDTLNPNEADQFSSEEVEKNEANSSCNESGGDARNSRDRLSSSHSEDEQLNQEYSTSSSGSTIGNREQGSDVGKDTSSRNEADQFSNEEVEKNEANSSGNESYGDACNSSDRLSSSNSEDEQLHQEYSTSSRGRTREYAQSDLNPGYQDLNIGNQDLNQVNREYQESNNEYQVSNQEYQHLNTEYQELNHGDQDSNDEYQELNHGNQDSNDEYQESNHGNQDSNDEYQELNHSNQDSNDEYQELNHGDQDSSDEYQDLNHVNQDSNHEYQAWNHEYQDSNHEYQALNREYQYPESDEEEAARKRNIYTRLRSYACQVHLPRLGESEVDSDDRRHNPINQPFNQPINRPNQKSDSSLESSSPERQDSESESRQASDEESSSGDSDYLIDPVTGWLSRRRETSTEREEKSDQSQQESSEEITDEQKEESDDVEQQSPAEHTSSDRRDHSPDKQQNKRKSLGTGNSSYSKKPLLDGDKGDPRSHLSSLGSRRSLKRKWPFDCTDSKKFKPNLQSEEDKSEEEKREDTESECSDEEDSDSSSFDTDSNSDSDSNLDLDSDSDSHSDFESSSSSEESAQDPAPEGETDQDPAPEGEADQDPAPEGGVDQDPAPEGGVDQDPAPEGEVDQDPAPEGESDQDLAPEGGVDQDPAPEFGTDQDPHPYYNDQDPAPYYDDQDPASYYDDHICDSEEDYKPFIFSKERKFLKFVTNPLGDDKSSLDLSIQLYKVFGAYKLEDVYSSIDEEGQPEQDLQLKQQQKHLKKYQKLQLVVFWHQEKFFSKEQLLLYEYLKLLLQKFFPQEQQEKRLQQEKLLQQEKLLQQEKRLQQEKRLQQEKRLQQEKRLQQEKRLQQQKLLQQQKLLQQEKLLQQQKLLQQEKLLQQEKLLQQEKQLQQEKHLPQERQLQQDKVFLQLRQLQQKKELEEQRERKRHLFEQQKQFVLEKLRQQKEQHRRQQNRVKRERKLRQKKEKQRLEYERFLKEYREDCKRQLRLQREHYLIKIKQLEKASQRSLKGNRKHKLMQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTNRQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLATGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDEEESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVLGTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASVNHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNIFVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEKANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINAASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQQQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF

Target ORF information:

RefSeq Version	NM_001169230.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169230.2

ORF Insert Sequence:

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAGTGAT 
CGCTTAAGTT CTTCTAATAG CGAGGACGAA CAGCTGCACC AAGAATATTC AACTTCATCA 
AGAGGAAGAA CAAGGGAATA TGCACAATCT GACTTGAATC CCGGGTACCA GGACTTGAAT 
ATCGGTAACC AGGACTTGAA TCAAGTTAAT CGCGAGTACC AGGAGTCTAA TAACGAGTAC 
CAGGTTTCAA ACCAAGAGTA CCAGCACTTG AATACCGAGT ACCAGGAGTT GAATCACGGC 
GATCAGGACT CAAATGACGA GTACCAGGAG TTGAATCACG GCAACCAGGA CTCAAATGAC 
GAGTACCAGG AATCAAATCA CGGCAACCAG GACTCAAATG ACGAGTACCA GGAGTTGAAT 
CACAGCAACC AGGACTCAAA TGACGAGTAC CAGGAGTTGA ATCACGGCGA TCAGGACTCA 
AGTGACGAGT ACCAGGATTT GAATCACGTC AACCAAGACT CAAATCACGA GTACCAGGCT 
TGGAATCACG AATACCAGGA CTCAAATCAC GAGTATCAGG CCTTGAATCG AGAGTACCAA 
TATCCTGAAT CTGATGAGGA AGAGGCCGCT AGAAAAAGAA ACATATATAC CCGTTTACGA 
TCCTACGCTT GTCAGGTACA TCTCCCTCGT TTAGGAGAAT CAGAAGTTGA TAGCGACGAT 
CGTCGCCATA ACCCAATCAA TCAGCCATTC AATCAGCCAA TCAATAGGCC AAATCAAAAA 
TCGGATTCAT CTTTAGAGTC ATCTTCGCCG GAGAGACAAG ACTCTGAAAG CGAGTCGCGA 
CAAGCTTCAG ATGAGGAATC GTCTTCTGGG GACTCGGACT ACTTAATCGA TCCAGTTACT 
GGGTGGCTAT CGCGCCGGCG AGAAACTTCT ACCGAACGCG AGGAAAAAAG CGACCAAAGC 
CAACAGGAGT CGTCAGAGGA AATTACAGAC GAACAGAAGG AAGAAAGCGA CGACGTCGAA 
CAACAGTCGC CGGCTGAACA TACTTCTTCT GATAGACGCG ATCATTCGCC AGACAAGCAA 
CAGAATAAGC GAAAATCGTT AGGCACTGGG AATTCTTCGT ATTCGAAGAA ACCATTATTG 
GACGGTGACA AAGGAGACCC ACGTTCACAT TTATCGTCTC TGGGAAGTCG CAGATCCTTA 
AAGCGTAAAT GGCCTTTTGA TTGTACAGAC TCTAAGAAAT TTAAACCAAA CTTGCAGAGT 
GAGGAAGACA AATCTGAGGA GGAAAAACGT GAAGATACGG AAAGCGAATG CTCTGACGAA 
GAAGACTCAG ATAGTTCAAG TTTCGATACA GATTCAAATT CAGATTCAGA TTCAAATTTA 
GATTTAGATT CCGATTCCGA TTCCCATTCC GATTTCGAAT CATCTTCAAG TTCTGAGGAA 
TCTGCCCAGG ATCCAGCTCC TGAAGGCGAA ACCGATCAAG ATCCAGCTCC TGAAGGCGAA 
GCCGATCAAG ATCCAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAAGGCGGA 
GTCGATCAAG ATCCAGCTCC TGAAGGCGAA GTCGATCAAG ATCCAGCTCC TGAAGGCGAA 
TCCGATCAAG ATCTAGCTCC TGAAGGCGGA GTCGATCAAG ATCCAGCTCC TGAATTCGGA 
ACCGATCAAG ATCCACATCC TTATTACAAC GATCAGGATC CAGCTCCTTA TTACGACGAT 
CAGGATCCAG CTTCTTATTA CGACGATCAC ATTTGTGATT CTGAGGAGGA TTATAAACCA 
TTTATCTTCT CAAAGGAAAG GAAGTTCTTG AAATTCGTAA CAAATCCTTT AGGGGATGAT 
AAATCTAGTT TGGACCTTAG CATACAGCTC TATAAGGTAT TTGGAGCTTA CAAACTGGAA 
GACGTATACA GCAGTATTGA TGAGGAAGGC CAACCAGAAC AGGACCTGCA ACTGAAGCAG 
CAACAGAAAC ATTTAAAAAA ATATCAAAAG TTGCAACTGG TGGTATTCTG GCACCAGGAG 
AAATTCTTTT CAAAGGAGCA ACTGTTGCTA TATGAGTATC TGAAACTACT TTTGCAGAAA 
TTCTTTCCAC AAGAGCAACA GGAGAAACGG TTGCAACAGG AGAAACTTTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACGGTTGCAA CAGGAGAAAC GGTTGCAACA GGAGAAACGG 
TTGCAACAGG AGAAACGGTT GCAACAGGAG AAACGGTTGC AACAGCAGAA ACTCTTGCAA 
CAGCAGAAAC TCTTGCAACA GGAGAAACTC TTGCAACAGC AGAAACTCTT GCAACAGGAG 
AAACTCTTGC AACAGGAGAA ACTCTTGCAA CAGGAGAAAC AATTGCAACA GGAGAAACAC 
TTGCCACAGG AGCGACAGTT GCAACAGGAT AAAGTCTTCC TACAGCTACG ACAGTTGCAA 
CAGAAGAAAG AACTGGAGGA GCAGCGAGAG CGAAAGCGCC ACCTCTTTGA GCAACAGAAA 
CAGTTTGTCC TAGAAAAATT GCGCCAACAG AAGGAGCAGC ATCGTCGTCA GCAAAATCGC 
GTAAAACGCG AACGTAAATT GCGCCAGAAA AAGGAGAAAC AGCGCTTGGA ATACGAACGA 
TTCTTGAAGG AATATAGGGA GGACTGTAAA CGTCAGTTGC GTTTGCAGCG CGAGCATTAT 
CTGATCAAGA TTAAGCAGCT GGAGAAGGCC AGTCAGCGCT CGTTGAAGGG GAATCGAAAG 
CACAAGCTGA TGCAAAACGA TATGGTCAGC ATACCGGTGG CCAATTTGAA CGGCGGCCTT 
AAAGCGGCCA GCAGCGGTTC CGGAGTTGGT GTAGTGACCT CCGGCGGCGT CGTATCCTCG 
GCGGTGCTGG CCAATGCCCC ACGTGTATAC CTAACGCCCT CCTCCACATT CATGACCAAT 
CGCCAAATGG CGGGAGTGGC GTCCACCGGA AGAATGAGCG GACAAGTCGT CGGTGGTTCA 
TCTGGCACAG CCTCAACAGC CGGTACCGTT CGCTATTTTT CGCAATTCAG CAAAATGCAA 
ACGGCCGGCG GACCCAGTTT GCAAAGGAAA CTTGCCAACG GGGATACCAT TGTTCTGGCA 
ACCGGCAGTA AAAATATGTT CCTTACTAGC AGCGAAAATA AGGCTAATCT GCCGACCGTC 
GCCAGCAATG GCAATGGTCT GATCACAGCC AAAATGGATC TATTGGAGGA GGAGGTCATG 
CAGTCGATAA CTGTGATTGA TGACGATGAC GAGGAGAAAA AGGAAGTCGC TGAAGACGAA 
GAAGAGAGCA GCAATAATGC CAAACCCATT GACCTTCACC AGCCAATTGC TGATAATGAG 
CACGAGCTGG ACATTGTGAT AAACAATGTG GTCTGCTCGT TTAGCGTCGG CTGTCACCTG 
AAGCTGCGCG AAATTGCGCT GCAGGGATCA AATGTGGAGT ACCGTCGCGA GAACGGAATG 
GTGACAATGA AGCTCCGCCA TCCATACACC ACTGCCTCGA TTTGGTCGTC GGGGAGGATT 
ACCTGCACTG GTGCCACTTC CGAGTCAATG GCCAAGGTCG CTGCACGCCG CTATGCCAGA 
TGCCTTGGCA AGCTGGGATT TCCCACTCGT TTCCTCAACT TTCGCATTGT CAACGTACTG 
GGCACCTGCA GCATGCCGTG GGCCATCAAG ATCGTCAACT TTTCGGAGCG CCATCGCGAG 
AACGCCAGCT ACGAGCCCGA GCTGCATCCT GGAGTGACTT ACAAGATGCG CGATCCCGAT 
CCAAAGGCCA CCCTGAAGAT CTTCTCCACC GGCAGCGTAA CAGTCACCGC GGCTAGCGTA 
AACCACGTTG AATCGGCTAT CCAGCACATA TACCCGCTGG TCTTTGATTT CCGCAAGCAA 
CGTTCGGCGG AGGAGCTGCA GCATCTGCGT CAAAAGCAGC GTCTGCAAGC CGGCGGTGAT 
CCCCACGAAC TGGAGAAAAA TGTCTTGGCC GATAACAAGA CTGCTTCCCT TGATAATATC 
TTTGTTAATA CAACAGCGGC GCACTCTAAA TCATCGTCGA ATGACCAAAC ATCAGCACCC 
GCGACCATCT TGTCCAGCAC AGTTGATAGT ATGCCTCGTC TGAAACAGAT GGTCAACTAT 
CACCAGATGA TGAAGCAAAC CCAGGAAGAG CGCCGCCATA TAATGTTTAA TGGGGAGAAG 
GCCAATCCGG CCTCCACCTC ATCGGCGGCA GCAGCTCCCT CCACCTCCTC CTCATCGTCC 
TCATCCGGGG ATAATATCTG TGCCAATGCC CGTCGCCGGG CCACCGAATG CTGGGCAACC 
AAGCTGCAGA ATAAGCGTCC ACGTTACAAT GATCCCGGTA CCACGGGCAC AATTAACGCC 
GCATCGTCCA CAGCATCAGC CGCCACATCT TCGTTAGCCT CCCAGGCGAC ACATCTGCGT 
AATCCTCTCA AGACGGCCGC GTTGGCCAAT GCACGTATGT TAGGCGCCAA GGTGACAACA 
TGCACACGCA ACAGCATAAT CGTGCAGCAG CCGCAACGTA TCCAAATGCA GCAGCAGCAG 
CAACAGCTGC AACCGCAGCA GCAACAGACC AGCTTCTCGC CCAGCGAATT CGATGTTGAT 
GACTTGATCG AGGAGGAGGA GAACAACGAA TTGGATATGC CCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf2 ( NM_001272400.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259329.1 Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant I, mRNA.

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CloneID	OFa03282
Clone ID Related Accession (Same CDS sequence)	NM_001272400.1
Accession Version	NM_001272400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	TATA box binding protein-related factor 2, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAATCGC 
TTAAGTTCTT CTAATAGCGA GGACGAACAG CTGCACCAAG AATATTCAAC TTCATCAAGA 
GGAAGAACAA GGGAATATGC ACAATCTGAC TTGAATCCCG GGTACCAGGA CTTGAATATC 
GGTAACCAGG ACTTGAATCA AGTTAATCGC GAGTACCAGG AGTCTAATAA CGAGTACCAG 
GTTTCAAACC AAGAGTACCA GCACTTGAAT ACCGAGTACC AGGAGTTGAA TCACGGCGAT 
CAGGACTCAA ATGACGAGTA CCAGGAGTTG AATCACGGCA ACCAGGACTC AAATGACGAG 
TACCAGGAAT CAAATCACGG CAACCAGGAC TCAAATGACG AGTACCAGGA GTTGAATCAC 
AGCAACCAGG ACTCAAATGA CGAGTACCAG GAGTTGAATC ACGGCGATCA GGACTCAAGT 
GACGAGTACC AGGATTTGAA TCACGTCAAC CAAGACTCAA ATCACGAGTA CCAGGCTTGG 
AATCACGAAT ACCAGGACTC AAATCACGAG TATCAGGCCT TGAATCGAGA GTACCAATAT 
CCTGAATCTG ATGAGGAAGA GGCCGCTAGA AAAAGAAACA TATATACCCG TTTACGATCC 
TACGCTTGTC AGGTACATCT CCCTCGTTTA GGAGAATCAG AAGTTGATAG CGACGATCGT 
CGCCATAACC CAATCAATCA GCCATTCAAT CAGCCAATCA ATAGGCCAAA TCAAAAATCG 
GATTCATCTT TAGAGTCATC TTCGCCGGAG AGACAAGACT CTGAAAGCGA GTCGCGACAA 
GCTTCAGATG AGGAATCGTC TTCTGGGGAC TCGGACTACT TAATCGATCC AGTTACTGGG 
TGGCTATCGC GCCGGCGAGA AACTTCTACC GAACGCGAGG AAAAAAGCGA CCAAAGCCAA 
CAGGAGTCGT CAGAGGAAAT TACAGACGAA CAGAAGGAAG AAAGCGACGA CGTCGAACAA 
CAGTCGCCGG CTGAACATAC TTCTTCTGAT AGACGCGATC ATTCGCCAGA CAAGCAACAG 
AATAAGCGAA AATCGTTAGG CACTGGGAAT TCTTCGTATT CGAAGAAACC ATTATTGGAC 
GGTGACAAAG GAGACCCACG TTCACATTTA TCGTCTCTGG GAAGTCGCAG ATCCTTAAAG 
CGTAAATGGC CTTTTGATTG TACAGACTCT AAGAAATTTA AACCAAACTT GCAGAGTGAG 
GAAGACAAAT CTGAGGAGGA AAAACGTGAA GATACGGAAA GCGAATGCTC TGACGAAGAA 
GACTCAGATA GTTCAAGTTT CGATACAGAT TCAAATTCAG ATTCAGATTC AAATTTAGAT 
TTAGATTCCG ATTCCGATTC CCATTCCGAT TTCGAATCAT CTTCAAGTTC TGAGGAATCT 
GCCCAGGATC CAGCTCCTGA AGGCGAAACC GATCAAGATC CAGCTCCTGA AGGCGAAGCC 
GATCAAGATC CAGCTCCTGA AGGCGGAGTC GATCAAGATC CAGCTCCTGA AGGCGGAGTC 
GATCAAGATC CAGCTCCTGA AGGCGAAGTC GATCAAGATC CAGCTCCTGA AGGCGAATCC 
GATCAAGATC TAGCTCCTGA AGGCGGAGTC GATCAAGATC CAGCTCCTGA ATTCGGAACC 
GATCAAGATC CACATCCTTA TTACAACGAT CAGGATCCAG CTCCTTATTA CGACGATCAG 
GATCCAGCTT CTTATTACGA CGATCACATT TGTGATTCTG AGGAGGATTA TAAACCATTT 
ATCTTCTCAA AGGAAAGGAA GTTCTTGAAA TTCGTAACAA ATCCTTTAGG GGATGATAAA 
TCTAGTTTGG ACCTTAGCAT ACAGCTCTAT AAGGTATTTG GAGCTTACAA ACTGGAAGAC 
GTATACAGCA GTATTGATGA GGAAGGCCAA CCAGAACAGG ACCTGCAACT GAAGCAGCAA 
CAGAAACATT TAAAAAAATA TCAAAAGTTG CAACTGGTGG TATTCTGGCA CCAGGAGAAA 
TTCTTTTCAA AGGAGCAACT GTTGCTATAT GAGTATCTGA AACTACTTTT GCAGAAATTC 
TTTCCACAAG AGCAACAGGA GAAACGGTTG CAACAGGAGA AACTTTTGCA ACAGGAGAAA 
CTCTTGCAAC AGGAGAAACG GTTGCAACAG GAGAAACGGT TGCAACAGGA GAAACGGTTG 
CAACAGGAGA AACGGTTGCA ACAGGAGAAA CGGTTGCAAC AGCAGAAACT CTTGCAACAG 
CAGAAACTCT TGCAACAGGA GAAACTCTTG CAACAGCAGA AACTCTTGCA ACAGGAGAAA 
CTCTTGCAAC AGGAGAAACT CTTGCAACAG GAGAAACAAT TGCAACAGGA GAAACACTTG 
CCACAGGAGC GACAGTTGCA ACAGGATAAA GTCTTCCTAC AGCTACGACA GTTGCAACAG 
AAGAAAGAAC TGGAGGAGCA GCGAGAGCGA AAGCGCCACC TCTTTGAGCA ACAGAAACAG 
TTTGTCCTAG AAAAATTGCG CCAACAGAAG GAGCAGCATC GTCGTCAGCA AAATCGCGTA 
AAACGCGAAC GTAAATTGCG CCAGAAAAAG GAGAAACAGC GCTTGGAATA CGAACGATTC 
TTGAAGGAAT ATAGGGAGGA CTGTAAACGT CAGTTGCGTT TGCAGCGCGA GCATTATCTG 
ATCAAGATTA AGCAGCTGGA GAAGGCCAGT CAGCGCTCGT TGAAGGGGAA TCGAAAGCAC 
AAGCTGATGC AAAACGATAT GGTCAGCATA CCGGTGGCCA ATTTGAACGG CGGCCTTAAA 
GCGGCCAGCA GCGGTTCCGG AGTTGGTGTA GTGACCTCCG GCGGCGTCGT ATCCTCGGCG 
GTGCTGGCCA ATGCCCCACG TGTATACCTA ACGCCCTCCT CCACATTCAT GACCAATCGC 
CAAATGGCGG GAGTGGCGTC CACCGGAAGA ATGAGCGGAC AAGTCGTCGG TGGTTCATCT 
GGCACAGCCT CAACAGCCGG TACCGTTCGC TATTTTTCGC AATTCAGCAA AATGCAAACG 
GCCGGCGGAC CCAGTTTGCA AAGGAAACTT GCCAACGGGG ATACCATTGT TCTGGCAACC 
GGCAGTAAAA ATATGTTCCT TACTAGCAGC GAAAATAAGG CTAATCTGCC GACCGTCGCC 
AGCAATGGCA ATGGTCTGAT CACAGCCAAA ATGGATCTAT TGGAGGAGGA GGTCATGCAG 
TCGATAACTG TGATTGATGA CGATGACGAG GAGAAAAAGG AAGTCGCTGA AGACGAAGAA 
GAGAGCAGCA ATAATGCCAA ACCCATTGAC CTTCACCAGC CAATTGCTGA TAATGAGCAC 
GAGCTGGACA TTGTGATAAA CAATGTGGTC TGCTCGTTTA GCGTCGGCTG TCACCTGAAG 
CTGCGCGAAA TTGCGCTGCA GGGATCAAAT GTGGAGTACC GTCGCGAGAA CGGAATGGTG 
ACAATGAAGC TCCGCCATCC ATACACCACT GCCTCGATTT GGTCGTCGGG GAGGATTACC 
TGCACTGGTG CCACTTCCGA GTCAATGGCC AAGGTCGCTG CACGCCGCTA TGCCAGATGC 
CTTGGCAAGC TGGGATTTCC CACTCGTTTC CTCAACTTTC GCATTGTCAA CGTACTGGGC 
ACCTGCAGCA TGCCGTGGGC CATCAAGATC GTCAACTTTT CGGAGCGCCA TCGCGAGAAC 
GCCAGCTACG AGCCCGAGCT GCATCCTGGA GTGACTTACA AGATGCGCGA TCCCGATCCA 
AAGGCCACCC TGAAGATCTT CTCCACCGGC AGCGTAACAG TCACCGCGGC TAGCGTAAAC 
CACGTTGAAT CGGCTATCCA GCACATATAC CCGCTGGTCT TTGATTTCCG CAAGCAACGT 
TCGGCGGAGG AGCTGCAGCA TCTGCGTCAA AAGCAGCGTC TGCAAGCCGG CGGTGATCCC 
CACGAACTGG AGAAAAATGT CTTGGCCGAT AACAAGACTG CTTCCCTTGA TAATATCTTT 
GTTAATACAA CAGCGGCGCA CTCTAAATCA TCGTCGAATG ACCAAACATC AGCACCCGCG 
ACCATCTTGT CCAGCACAGT TGATAGTATG CCTCGTCTGA AACAGATGGT CAACTATCAC 
CAGATGATGA AGCAAACCCA GGAAGAGCGC CGCCATATAA TGTTTAATGG GGAGAAGGCC 
AATCCGGCCT CCACCTCATC GGCGGCAGCA GCTCCCTCCA CCTCCTCCTC ATCGTCCTCA 
TCCGGGGATA ATATCTGTGC CAATGCCCGT CGCCGGGCCA CCGAATGCTG GGCAACCAAG 
CTGCAGAATA AGCGTCCACG TTACAATGAT CCCGGTACCA CGGGCACAAT TAACGCCGCA 
TCGTCCACAG CATCAGCCGC CACATCTTCG TTAGCCTCCC AGGCGACACA TCTGCGTAAT 
CCTCTCAAGA CGGCCGCGTT GGCCAATGCA CGTATGTTAG GCGCCAAGGT GACAACATGC 
ACACGCAACA GCATAATCGT GCAGCAGCCG CAACGTATCC AAATGCAGCA GCAGCAGCAA 
CAGCTGCAAC CGCAGCAGCA ACAGACCAGC TTCTCGCCCA GCGAATTCGA TGTTGATGAC 
TTGATCGAGG AGGAGGAGAA CAACGAATTG GATATGCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259329.1
CDS	936..6080
Translation	MGSAVKKGDGSPTRILEEYQVARDLRNEDLFPEHPVEHPDEDSDKENQSPNQSPNPRAIGDCEQGPDIGKDTLNPNEADQFSSEEVEKNEANSSCNESGGDARNSRDRLSSSHSEDEQLNQEYSTSSSGSTIGNREQGSDVGKDTSSRNEADQFSNEEVEKNEANSSGNESYGDACNSNRLSSSNSEDEQLHQEYSTSSRGRTREYAQSDLNPGYQDLNIGNQDLNQVNREYQESNNEYQVSNQEYQHLNTEYQELNHGDQDSNDEYQELNHGNQDSNDEYQESNHGNQDSNDEYQELNHSNQDSNDEYQELNHGDQDSSDEYQDLNHVNQDSNHEYQAWNHEYQDSNHEYQALNREYQYPESDEEEAARKRNIYTRLRSYACQVHLPRLGESEVDSDDRRHNPINQPFNQPINRPNQKSDSSLESSSPERQDSESESRQASDEESSSGDSDYLIDPVTGWLSRRRETSTEREEKSDQSQQESSEEITDEQKEESDDVEQQSPAEHTSSDRRDHSPDKQQNKRKSLGTGNSSYSKKPLLDGDKGDPRSHLSSLGSRRSLKRKWPFDCTDSKKFKPNLQSEEDKSEEEKREDTESECSDEEDSDSSSFDTDSNSDSDSNLDLDSDSDSHSDFESSSSSEESAQDPAPEGETDQDPAPEGEADQDPAPEGGVDQDPAPEGGVDQDPAPEGEVDQDPAPEGESDQDLAPEGGVDQDPAPEFGTDQDPHPYYNDQDPAPYYDDQDPASYYDDHICDSEEDYKPFIFSKERKFLKFVTNPLGDDKSSLDLSIQLYKVFGAYKLEDVYSSIDEEGQPEQDLQLKQQQKHLKKYQKLQLVVFWHQEKFFSKEQLLLYEYLKLLLQKFFPQEQQEKRLQQEKLLQQEKLLQQEKRLQQEKRLQQEKRLQQEKRLQQEKRLQQQKLLQQQKLLQQEKLLQQQKLLQQEKLLQQEKLLQQEKQLQQEKHLPQERQLQQDKVFLQLRQLQQKKELEEQRERKRHLFEQQKQFVLEKLRQQKEQHRRQQNRVKRERKLRQKKEKQRLEYERFLKEYREDCKRQLRLQREHYLIKIKQLEKASQRSLKGNRKHKLMQNDMVSIPVANLNGGLKAASSGSGVGVVTSGGVVSSAVLANAPRVYLTPSSTFMTNRQMAGVASTGRMSGQVVGGSSGTASTAGTVRYFSQFSKMQTAGGPSLQRKLANGDTIVLATGSKNMFLTSSENKANLPTVASNGNGLITAKMDLLEEEVMQSITVIDDDDEEKKEVAEDEEESSNNAKPIDLHQPIADNEHELDIVINNVVCSFSVGCHLKLREIALQGSNVEYRRENGMVTMKLRHPYTTASIWSSGRITCTGATSESMAKVAARRYARCLGKLGFPTRFLNFRIVNVLGTCSMPWAIKIVNFSERHRENASYEPELHPGVTYKMRDPDPKATLKIFSTGSVTVTAASVNHVESAIQHIYPLVFDFRKQRSAEELQHLRQKQRLQAGGDPHELEKNVLADNKTASLDNIFVNTTAAHSKSSSNDQTSAPATILSSTVDSMPRLKQMVNYHQMMKQTQEERRHIMFNGEKANPASTSSAAAAPSTSSSSSSSGDNICANARRRATECWATKLQNKRPRYNDPGTTGTINAASSTASAATSSLASQATHLRNPLKTAALANARMLGAKVTTCTRNSIIVQQPQRIQMQQQQQQLQPQQQQTSFSPSEFDVDDLIEEEENNELDMPF

Target ORF information:

RefSeq Version	NM_001272400.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster TATA box binding protein-related factor 2 (Trf2), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272400.1

ORF Insert Sequence:

ATGGGCAGTG CCGTCAAAAA GGGGGATGGT TCCCCGACTC GAATACTTGA AGAATACCAA 
GTAGCTAGAG ACTTAAGGAA CGAGGATTTG TTTCCGGAAC ATCCGGTTGA ACATCCGGAC 
GAAGATTCGG ATAAGGAGAA CCAATCGCCG AATCAATCCC CCAATCCAAG AGCCATCGGT 
GATTGTGAGC AAGGACCTGA CATTGGTAAA GATACTCTAA ATCCCAACGA AGCTGACCAG 
TTTTCGAGCG AAGAAGTTGA GAAGAACGAA GCCAACAGCA GCTGTAACGA GTCGGGCGGG 
GATGCTCGCA ATTCTAGAGA TCGTTTAAGT TCCTCTCATA GCGAGGACGA ACAGCTTAAC 
CAAGAATATT CAACTTCATC AAGCGGAAGC ACCATCGGAA ATCGTGAGCA AGGCTCTGAC 
GTTGGTAAAG ATACTTCAAG TCGCAACGAA GCTGACCAGT TTTCGAACGA AGAAGTTGAG 
AAGAACGAAG CCAACAGCAG CGGTAACGAG TCGTACGGGG ATGCCTGCAA TTCTAATCGC 
TTAAGTTCTT CTAATAGCGA GGACGAACAG CTGCACCAAG AATATTCAAC TTCATCAAGA 
GGAAGAACAA GGGAATATGC ACAATCTGAC TTGAATCCCG GGTACCAGGA CTTGAATATC 
GGTAACCAGG ACTTGAATCA AGTTAATCGC GAGTACCAGG AGTCTAATAA CGAGTACCAG 
GTTTCAAACC AAGAGTACCA GCACTTGAAT ACCGAGTACC AGGAGTTGAA TCACGGCGAT 
CAGGACTCAA ATGACGAGTA CCAGGAGTTG AATCACGGCA ACCAGGACTC AAATGACGAG 
TACCAGGAAT CAAATCACGG CAACCAGGAC TCAAATGACG AGTACCAGGA GTTGAATCAC 
AGCAACCAGG ACTCAAATGA CGAGTACCAG GAGTTGAATC ACGGCGATCA GGACTCAAGT 
GACGAGTACC AGGATTTGAA TCACGTCAAC CAAGACTCAA ATCACGAGTA CCAGGCTTGG 
AATCACGAAT ACCAGGACTC AAATCACGAG TATCAGGCCT TGAATCGAGA GTACCAATAT 
CCTGAATCTG ATGAGGAAGA GGCCGCTAGA AAAAGAAACA TATATACCCG TTTACGATCC 
TACGCTTGTC AGGTACATCT CCCTCGTTTA GGAGAATCAG AAGTTGATAG CGACGATCGT 
CGCCATAACC CAATCAATCA GCCATTCAAT CAGCCAATCA ATAGGCCAAA TCAAAAATCG 
GATTCATCTT TAGAGTCATC TTCGCCGGAG AGACAAGACT CTGAAAGCGA GTCGCGACAA 
GCTTCAGATG AGGAATCGTC TTCTGGGGAC TCGGACTACT TAATCGATCC AGTTACTGGG 
TGGCTATCGC GCCGGCGAGA AACTTCTACC GAACGCGAGG AAAAAAGCGA CCAAAGCCAA 
CAGGAGTCGT CAGAGGAAAT TACAGACGAA CAGAAGGAAG AAAGCGACGA CGTCGAACAA 
CAGTCGCCGG CTGAACATAC TTCTTCTGAT AGACGCGATC ATTCGCCAGA CAAGCAACAG 
AATAAGCGAA AATCGTTAGG CACTGGGAAT TCTTCGTATT CGAAGAAACC ATTATTGGAC 
GGTGACAAAG GAGACCCACG TTCACATTTA TCGTCTCTGG GAAGTCGCAG ATCCTTAAAG 
CGTAAATGGC CTTTTGATTG TACAGACTCT AAGAAATTTA AACCAAACTT GCAGAGTGAG 
GAAGACAAAT CTGAGGAGGA AAAACGTGAA GATACGGAAA GCGAATGCTC TGACGAAGAA 
GACTCAGATA GTTCAAGTTT CGATACAGAT TCAAATTCAG ATTCAGATTC AAATTTAGAT 
TTAGATTCCG ATTCCGATTC CCATTCCGAT TTCGAATCAT CTTCAAGTTC TGAGGAATCT 
GCCCAGGATC CAGCTCCTGA AGGCGAAACC GATCAAGATC CAGCTCCTGA AGGCGAAGCC 
GATCAAGATC CAGCTCCTGA AGGCGGAGTC GATCAAGATC CAGCTCCTGA AGGCGGAGTC 
GATCAAGATC CAGCTCCTGA AGGCGAAGTC GATCAAGATC CAGCTCCTGA AGGCGAATCC 
GATCAAGATC TAGCTCCTGA AGGCGGAGTC GATCAAGATC CAGCTCCTGA ATTCGGAACC 
GATCAAGATC CACATCCTTA TTACAACGAT CAGGATCCAG CTCCTTATTA CGACGATCAG 
GATCCAGCTT CTTATTACGA CGATCACATT TGTGATTCTG AGGAGGATTA TAAACCATTT 
ATCTTCTCAA AGGAAAGGAA GTTCTTGAAA TTCGTAACAA ATCCTTTAGG GGATGATAAA 
TCTAGTTTGG ACCTTAGCAT ACAGCTCTAT AAGGTATTTG GAGCTTACAA ACTGGAAGAC 
GTATACAGCA GTATTGATGA GGAAGGCCAA CCAGAACAGG ACCTGCAACT GAAGCAGCAA 
CAGAAACATT TAAAAAAATA TCAAAAGTTG CAACTGGTGG TATTCTGGCA CCAGGAGAAA 
TTCTTTTCAA AGGAGCAACT GTTGCTATAT GAGTATCTGA AACTACTTTT GCAGAAATTC 
TTTCCACAAG AGCAACAGGA GAAACGGTTG CAACAGGAGA AACTTTTGCA ACAGGAGAAA 
CTCTTGCAAC AGGAGAAACG GTTGCAACAG GAGAAACGGT TGCAACAGGA GAAACGGTTG 
CAACAGGAGA AACGGTTGCA ACAGGAGAAA CGGTTGCAAC AGCAGAAACT CTTGCAACAG 
CAGAAACTCT TGCAACAGGA GAAACTCTTG CAACAGCAGA AACTCTTGCA ACAGGAGAAA 
CTCTTGCAAC AGGAGAAACT CTTGCAACAG GAGAAACAAT TGCAACAGGA GAAACACTTG 
CCACAGGAGC GACAGTTGCA ACAGGATAAA GTCTTCCTAC AGCTACGACA GTTGCAACAG 
AAGAAAGAAC TGGAGGAGCA GCGAGAGCGA AAGCGCCACC TCTTTGAGCA ACAGAAACAG 
TTTGTCCTAG AAAAATTGCG CCAACAGAAG GAGCAGCATC GTCGTCAGCA AAATCGCGTA 
AAACGCGAAC GTAAATTGCG CCAGAAAAAG GAGAAACAGC GCTTGGAATA CGAACGATTC 
TTGAAGGAAT ATAGGGAGGA CTGTAAACGT CAGTTGCGTT TGCAGCGCGA GCATTATCTG 
ATCAAGATTA AGCAGCTGGA GAAGGCCAGT CAGCGCTCGT TGAAGGGGAA TCGAAAGCAC 
AAGCTGATGC AAAACGATAT GGTCAGCATA CCGGTGGCCA ATTTGAACGG CGGCCTTAAA 
GCGGCCAGCA GCGGTTCCGG AGTTGGTGTA GTGACCTCCG GCGGCGTCGT ATCCTCGGCG 
GTGCTGGCCA ATGCCCCACG TGTATACCTA ACGCCCTCCT CCACATTCAT GACCAATCGC 
CAAATGGCGG GAGTGGCGTC CACCGGAAGA ATGAGCGGAC AAGTCGTCGG TGGTTCATCT 
GGCACAGCCT CAACAGCCGG TACCGTTCGC TATTTTTCGC AATTCAGCAA AATGCAAACG 
GCCGGCGGAC CCAGTTTGCA AAGGAAACTT GCCAACGGGG ATACCATTGT TCTGGCAACC 
GGCAGTAAAA ATATGTTCCT TACTAGCAGC GAAAATAAGG CTAATCTGCC GACCGTCGCC 
AGCAATGGCA ATGGTCTGAT CACAGCCAAA ATGGATCTAT TGGAGGAGGA GGTCATGCAG 
TCGATAACTG TGATTGATGA CGATGACGAG GAGAAAAAGG AAGTCGCTGA AGACGAAGAA 
GAGAGCAGCA ATAATGCCAA ACCCATTGAC CTTCACCAGC CAATTGCTGA TAATGAGCAC 
GAGCTGGACA TTGTGATAAA CAATGTGGTC TGCTCGTTTA GCGTCGGCTG TCACCTGAAG 
CTGCGCGAAA TTGCGCTGCA GGGATCAAAT GTGGAGTACC GTCGCGAGAA CGGAATGGTG 
ACAATGAAGC TCCGCCATCC ATACACCACT GCCTCGATTT GGTCGTCGGG GAGGATTACC 
TGCACTGGTG CCACTTCCGA GTCAATGGCC AAGGTCGCTG CACGCCGCTA TGCCAGATGC 
CTTGGCAAGC TGGGATTTCC CACTCGTTTC CTCAACTTTC GCATTGTCAA CGTACTGGGC 
ACCTGCAGCA TGCCGTGGGC CATCAAGATC GTCAACTTTT CGGAGCGCCA TCGCGAGAAC 
GCCAGCTACG AGCCCGAGCT GCATCCTGGA GTGACTTACA AGATGCGCGA TCCCGATCCA 
AAGGCCACCC TGAAGATCTT CTCCACCGGC AGCGTAACAG TCACCGCGGC TAGCGTAAAC 
CACGTTGAAT CGGCTATCCA GCACATATAC CCGCTGGTCT TTGATTTCCG CAAGCAACGT 
TCGGCGGAGG AGCTGCAGCA TCTGCGTCAA AAGCAGCGTC TGCAAGCCGG CGGTGATCCC 
CACGAACTGG AGAAAAATGT CTTGGCCGAT AACAAGACTG CTTCCCTTGA TAATATCTTT 
GTTAATACAA CAGCGGCGCA CTCTAAATCA TCGTCGAATG ACCAAACATC AGCACCCGCG 
ACCATCTTGT CCAGCACAGT TGATAGTATG CCTCGTCTGA AACAGATGGT CAACTATCAC 
CAGATGATGA AGCAAACCCA GGAAGAGCGC CGCCATATAA TGTTTAATGG GGAGAAGGCC 
AATCCGGCCT CCACCTCATC GGCGGCAGCA GCTCCCTCCA CCTCCTCCTC ATCGTCCTCA 
TCCGGGGATA ATATCTGTGC CAATGCCCGT CGCCGGGCCA CCGAATGCTG GGCAACCAAG 
CTGCAGAATA AGCGTCCACG TTACAATGAT CCCGGTACCA CGGGCACAAT TAACGCCGCA 
TCGTCCACAG CATCAGCCGC CACATCTTCG TTAGCCTCCC AGGCGACACA TCTGCGTAAT 
CCTCTCAAGA CGGCCGCGTT GGCCAATGCA CGTATGTTAG GCGCCAAGGT GACAACATGC 
ACACGCAACA GCATAATCGT GCAGCAGCCG CAACGTATCC AAATGCAGCA GCAGCAGCAA 
CAGCTGCAAC CGCAGCAGCA ACAGACCAGC TTCTCGCCCA GCGAATTCGA TGTTGATGAC 
TTGATCGAGG AGGAGGAGAA CAACGAATTG GATATGCCCT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Trf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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