PIG-T cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIG-T
Entrez Gene ID	31772
Full Name	CG11190 gene product from transcript CG11190-RA
Synonyms	Dm*AAF46367/27-522,Dmel\CG11190
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7E6-7E6

mRNA and Protein(s)

mRNA	Protein	Name
NM_132247.4	NP_572475.1	phosphatidylinositol glycan anchor biosynthesis class T

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PIGT	XP_001155968.1
Canis lupus familiaris (dog)	PIGT	XP_534436.2
Rattus norvegicus (Norway rat)	Pigt	NP_001100010.2
Gallus gallus (chicken)	PIGT	XP_004947076.1
Danio rerio (zebrafish)	pigt	XP_684608.1
Saccharomyces cerevisiae (baker's yeast)	GPI16	NP_012058.1
Neurospora crassa	NCU05644	XP_960205.1
	PIGT	XP_001108481.1
Drosophila melanogaster (fruit fly)	CG11190	NP_572475.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008540	XP_314651.3
Kluyveromyces lactis	KLLA0A09515g	XP_451416.1
Magnaporthe oryzae (rice blast fungus)	MGG_03759	XP_003720129.1
Bos taurus (cattle)	PIGT	NP_001179925.1
Eremothecium gossypii	AGOS_ADR333C	NP_984429.2
Arabidopsis thaliana (thale cress)	AT3G07140	NP_566299.1
Xenopus tropicalis (tropical clawed frog)	pigt	NP_001017115.1
Homo sapiens (human)	PIGT	NP_057021.2
Mus musculus (house mouse)	Pigt	NP_598540.2
Oryza sativa (rice)	Os11g0479232	NP_001176538.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PIG-T gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIG-T cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01109	NM_132247.4 Latest version!	Drosophila melanogaster phosphatidylinositol glycan anchor biosynthesis class T (PIG-T), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIG-T ( NM_132247.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572475.1 Drosophila melanogaster phosphatidylinositol glycan anchor biosynthesis class T (PIG-T), mRNA.

Price & Availability↑

CloneID	OFa01109
Clone ID Related Accession (Same CDS sequence)	NM_132247.4
Accession Version	NM_132247.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	phosphatidylinositol glycan anchor biosynthesis class T
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132247.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATCCGG TGGCGTTTGT CTTGCTGCTG CTGGTTGCAC CTAATGCCGA CGCGAATTTG 
GGACGCGATG ACGAACGATT CCACGAGGAG CTAGTGGTTC GTCCATTGTC CGGCGACCAT 
GTCAACACCT ACTTTCAGTT CACCACGCGC TGGCACTACG GCGAAAAGGA TAATCTTTAC 
CACACCCAAT TGACGCCTCG AGTAATTGCG GAGCTGTTGC AGCAGTTTGC GGTAAAGGAG 
CTGCACATTG GCCTAACCCA GGGACTGTGG CGCTACGAGA CATGGGGCTA TCCCATTGTG 
GAGGCCACCA GCGGTGCCGA GATGTGGGCG TGGTTCAGTG GCGCAAATTT GACCAACCGG 
GATGTGGATC GCCAGTGGAA GGAGTTGGCC AACGTTTTCT CGGGCGTGTT GTGCGCCTCG 
TTGAATTTCG TCGACAATAC GAATAGCATT GCACCGAGGC ACTTGATTCG TCCACAATTT 
ATGCCAGCCA ATGGCCAAAG ATTCGTTCGC TATGCCACAT TGCCGCGTGA GATCGTGTGC 
ACGGAGAATC TGACGCCGTG GAAGAAACTA CTGCCATGCG GTAGTGCCTC AGGCTTTGCC 
TCACTTCTCA ACTCGGGACA TGTACACAAC ACCAAATACC ATTCGCTGGG CCTGAAGGTG 
CGCGTGCTCT GCGAGGATCA CGATGAGGAC AATTGCATTG TGGAGCTGAC GCAGACGGCC 
AACTTGGTCT ACGATCTCCG TCTGTTCGAA TTGAGCAACA ATGATTTCTC GCTGCGCCGC 
CTGTTCGGCA TGGGACTGAA TGGCTATTGT GAGCTGGCCG AGAGCTCCAA GATCTATGTG 
CAGCGCAACG AGCTGGGCGA GCGCTATCAG CTGGTTCCAG AACCCGTGCA CGAAGTGAAG 
ACCACTCGTG GTGGGCATAG TGTCGTCTAT TCCGTCTACG ATATGCACGA GCAGTTCAAA 
GAGGCCGGCG AACGGCTGTT TAATGTCGCC TGGCTGGCGC CGAAGAGCGC AAATCGTCGT 
CGCAATTTGG CGAAGCCATC ACTGCCACCG GTGACGGTTC ATCGTTATCT ATTGGGACAC 
GGCCAGGAGC GTGGCAGGAT CGTTACGGAG GTGACGAACT CGCACTACGA CGCCCTGCCC 
ATAATGCTGC AGGAGGTGAT ACCGTGGTAT GTGCACGCCT ATTTGCACAC CCTTTCCATA 
CGTCGCAAGC CGCAGCGGGT CAACGAATAC GGAGGCCAGC GATTGACCTT CAAGCTGCTC 
CACTATACGC CCGGCAAGCA GAGGGAACTG CCGTCCCATT TGGAAATTGG CTTCATGCTG 
CCCGGCCAGA CATCGGCGCT GATAAGCATC GATGTGGATT ATTTGCTGCT CAAATGGTTG 
GAGTATCCGC CGGATGCGAA TCACGGCCAT TACATCGGCT CGGCCATTGT GTCCAGCCAA 
CTGCCGATGG GCAGAAACTA TACCGCTCTG CCGCCGGAGG GGCATCTCTT TGAGCATTCG 
TTCAATGCCA CACGTCCGAG TTATGTTCTC AGTTTGCACA CGGAAGCGCT GATTGTCTCA 
CTGCCCACGC CGGACTTTAG CATGCCGTAC AATGTGATCT GTCTCGCATG CACAGTGGTT 
GCCCTGGCCT TTGGACCCAT ACACAGTGTG GCCACCAAGA TGATCATTGT CGGTCGTCAG 
ACATCGGCGC CGAAGAATTT TGTTAAGAAG ATCTTCAATC AGCTTTTCCG GCGCGGCAAG 
GCCACCGATG AGACAGCTGC CGAGGGCGAA GGATTGCCAG CGGCAGGAGC AGCAGCAACT 
GGAGTTGCAT CGGGGAGAGC AGCGATATCG CCAGGCAGTC CATCGGGCGA CCAGCCGCTG 
CTGGAAGATC TCGACGAGGA GGAGGAGGAG GAGCAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572475.1
CDS	295..2196
Translation	MYPVAFVLLLLVAPNADANLGRDDERFHEELVVRPLSGDHVNTYFQFTTRWHYGEKDNLYHTQLTPRVIAELLQQFAVKELHIGLTQGLWRYETWGYPIVEATSGAEMWAWFSGANLTNRDVDRQWKELANVFSGVLCASLNFVDNTNSIAPRHLIRPQFMPANGQRFVRYATLPREIVCTENLTPWKKLLPCGSASGFASLLNSGHVHNTKYHSLGLKVRVLCEDHDEDNCIVELTQTANLVYDLRLFELSNNDFSLRRLFGMGLNGYCELAESSKIYVQRNELGERYQLVPEPVHEVKTTRGGHSVVYSVYDMHEQFKEAGERLFNVAWLAPKSANRRRNLAKPSLPPVTVHRYLLGHGQERGRIVTEVTNSHYDALPIMLQEVIPWYVHAYLHTLSIRRKPQRVNEYGGQRLTFKLLHYTPGKQRELPSHLEIGFMLPGQTSALISIDVDYLLLKWLEYPPDANHGHYIGSAIVSSQLPMGRNYTALPPEGHLFEHSFNATRPSYVLSLHTEALIVSLPTPDFSMPYNVICLACTVVALAFGPIHSVATKMIIVGRQTSAPKNFVKKIFNQLFRRGKATDETAAEGEGLPAAGAAATGVASGRAAISPGSPSGDQPLLEDLDEEEEEEQD

Target ORF information:

RefSeq Version	NM_132247.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster phosphatidylinositol glycan anchor biosynthesis class T (PIG-T), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132247.4

ORF Insert Sequence:

ATGTATCCGG TGGCGTTTGT CTTGCTGCTG CTGGTTGCAC CTAATGCCGA CGCGAATTTG 
GGACGCGATG ACGAACGATT CCACGAGGAG CTAGTGGTTC GTCCATTGTC CGGCGACCAT 
GTCAACACCT ACTTTCAGTT CACCACGCGC TGGCACTACG GCGAAAAGGA TAATCTTTAC 
CACACCCAAT TGACGCCTCG AGTAATTGCG GAGCTGTTGC AGCAGTTTGC GGTAAAGGAG 
CTGCACATTG GCCTAACCCA GGGACTGTGG CGCTACGAGA CATGGGGCTA TCCCATTGTG 
GAGGCCACCA GCGGTGCCGA GATGTGGGCG TGGTTCAGTG GCGCAAATTT GACCAACCGG 
GATGTGGATC GCCAGTGGAA GGAGTTGGCC AACGTTTTCT CGGGCGTGTT GTGCGCCTCG 
TTGAATTTCG TCGACAATAC GAATAGCATT GCACCGAGGC ACTTGATTCG TCCACAATTT 
ATGCCAGCCA ATGGCCAAAG ATTCGTTCGC TATGCCACAT TGCCGCGTGA GATCGTGTGC 
ACGGAGAATC TGACGCCGTG GAAGAAACTA CTGCCATGCG GTAGTGCCTC AGGCTTTGCC 
TCACTTCTCA ACTCGGGACA TGTACACAAC ACCAAATACC ATTCGCTGGG CCTGAAGGTG 
CGCGTGCTCT GCGAGGATCA CGATGAGGAC AATTGCATTG TGGAGCTGAC GCAGACGGCC 
AACTTGGTCT ACGATCTCCG TCTGTTCGAA TTGAGCAACA ATGATTTCTC GCTGCGCCGC 
CTGTTCGGCA TGGGACTGAA TGGCTATTGT GAGCTGGCCG AGAGCTCCAA GATCTATGTG 
CAGCGCAACG AGCTGGGCGA GCGCTATCAG CTGGTTCCAG AACCCGTGCA CGAAGTGAAG 
ACCACTCGTG GTGGGCATAG TGTCGTCTAT TCCGTCTACG ATATGCACGA GCAGTTCAAA 
GAGGCCGGCG AACGGCTGTT TAATGTCGCC TGGCTGGCGC CGAAGAGCGC AAATCGTCGT 
CGCAATTTGG CGAAGCCATC ACTGCCACCG GTGACGGTTC ATCGTTATCT ATTGGGACAC 
GGCCAGGAGC GTGGCAGGAT CGTTACGGAG GTGACGAACT CGCACTACGA CGCCCTGCCC 
ATAATGCTGC AGGAGGTGAT ACCGTGGTAT GTGCACGCCT ATTTGCACAC CCTTTCCATA 
CGTCGCAAGC CGCAGCGGGT CAACGAATAC GGAGGCCAGC GATTGACCTT CAAGCTGCTC 
CACTATACGC CCGGCAAGCA GAGGGAACTG CCGTCCCATT TGGAAATTGG CTTCATGCTG 
CCCGGCCAGA CATCGGCGCT GATAAGCATC GATGTGGATT ATTTGCTGCT CAAATGGTTG 
GAGTATCCGC CGGATGCGAA TCACGGCCAT TACATCGGCT CGGCCATTGT GTCCAGCCAA 
CTGCCGATGG GCAGAAACTA TACCGCTCTG CCGCCGGAGG GGCATCTCTT TGAGCATTCG 
TTCAATGCCA CACGTCCGAG TTATGTTCTC AGTTTGCACA CGGAAGCGCT GATTGTCTCA 
CTGCCCACGC CGGACTTTAG CATGCCGTAC AATGTGATCT GTCTCGCATG CACAGTGGTT 
GCCCTGGCCT TTGGACCCAT ACACAGTGTG GCCACCAAGA TGATCATTGT CGGTCGTCAG 
ACATCGGCGC CGAAGAATTT TGTTAAGAAG ATCTTCAATC AGCTTTTCCG GCGCGGCAAG 
GCCACCGATG AGACAGCTGC CGAGGGCGAA GGATTGCCAG CGGCAGGAGC AGCAGCAACT 
GGAGTTGCAT CGGGGAGAGC AGCGATATCG CCAGGCAGTC CATCGGGCGA CCAGCCGCTG 
CTGGAAGATC TCGACGAGGA GGAGGAGGAG GAGCAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PIG-T cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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