nAChRalpha3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nAChRalpha3
Entrez Gene ID	31767
Full Name	nicotinic Acetylcholine Receptor alpha3
Synonyms	Acr7E,CG2302,D[a3],Da3,Dalpha3,Dalpha3 nAChR,Dmel\CG2302,alpha3,lincRNA.965,nACRalpha-7E,nAChR,nAcRalpha-7E,nAcRe,nicra3
General protein information	Preferred Names nicotinic Acetylcholine Receptor alpha3 Names CG2302 gene product from transcript CG2302-RC CG2302-PB CG2302-PC Dalpha3 drosophila alpha-like subunit 3 nAChRalpha3-PB nAChRalpha3-PC nAcRalpha-7E nicotinic acetylcholine receptor alpha 7E nicotinic acetylcholine receptor alpha3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7E1-7E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_080340.4	NP_525079.3	nicotinic acetylcholine receptor alpha3, isoform B
NM_001298086.1	NP_001285015.1	nicotinic acetylcholine receptor alpha3, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHRNA6	NP_001029266.1
Drosophila melanogaster (fruit fly)	nAcRalpha-7E	NP_525079.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000329	XP_310786.3
Homo sapiens (human)	CHRNA6	NP_004189.1
Bos taurus (cattle)	CHRNA6	XP_002698774.2
Gallus gallus (chicken)	CHRNA6	NP_990695.1
	CHRNA6	XP_001099152.1
Rattus norvegicus (Norway rat)	Chrna6	NP_476532.1
Caenorhabditis elegans (roundworm)	unc-38	NP_491472.1
Xenopus tropicalis (tropical clawed frog)	LOC100495287	XP_002942944.2
Canis lupus familiaris (dog)	CHRNA6	XP_539951.2
Mus musculus (house mouse)	Chrna6	NP_067344.2
Danio rerio (zebrafish)	chrna6	NP_001036149.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nAChRalpha3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nAChRalpha3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01106	NM_001298086.1 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha3, transcript variant C (nAChRalpha3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa01106	NM_080340.4 Latest version!	Drosophila melanogaster nicotinic acetylcholine receptor alpha3, transcript variant B (nAChRalpha3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nAChRalpha3 ( NM_080340.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525079.3 Drosophila melanogaster nicotinic acetylcholine receptor alpha3, transcript variant B (nAChRalpha3), mRNA.

Price & Availability↑

CloneID	OFa01106
Clone ID Related Accession (Same CDS sequence)	NM_080340.4 , NM_001298086.1
Accession Version	NM_080340.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha3, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080340.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCAAGTGAC CATAGATATG ATTCTTGTGC TTTCATTTAT ATCAAGCAGC 
ACAGCGAATC CGGATGCGAA ACGATTGTAC GACGATTTGC TGAGCAACTA CAACAAACTG 
GTACGACCGG TGGTCAATGT GACGGACGCG CTCACCGTTC GCATCAAGCT GAAGCTATCG 
CAACTGATCG ATGTCAATCT GAAGAACCAG ATAATGACCA CCAATCTGTG GGTGGAGCAG 
TCCTGGTACG ACTACAAGCT CAAGTGGGAG CCCAAGGAGT ATGGCGGCGT CGAGATGCTT 
CACGTGCCCT CCGATCACAT CTGGCGTCCG GACATCGTTC TCTACAACAA CGCCGATGGC 
AATTTCGAGG TGACGCTGGC CACCAAGGCC ACGTTGAATT ACACAGGACG CGTCGAGTGG 
CGACCGCCGG CGATTTACAA GAGCTCCTGC GAAATCGACG TGGAGTACTT TCCCTTCGAC 
GAGCAGACCT GCGTCATGAA GTTCGGCAGC TGGACATACG ATGGATTTCA GGTGGATCTG 
CGGCACATCG ACGAGCTGAA CGGCACCAAT GTCGTGGAGG TGGGCGTGGA TCTGTCCGAG 
TTCTATACGT CCGTCGAATG GGATATCCTC GAAGTTCCTG CAGTGCGAAA CGAGAAGTTC 
TACACGTGCT GCGATGAGCC ATATCTGGAC ATCACCTTCA ACATAACGAT GCGTCGGAAG 
ACTCTCTTCT ATACCGTCAA CCTGATCATT CCCTGCATGG GCATCAGTTT TCTCACCATC 
CTCGTATTCT ACCTGCCATC GGATAGTGGC GAGAAGGTCT CATTAAGCAT ATCCATTCTG 
CTGTCGCTCA CTGTGTTCTT CCTGCTGCTG GCGGAAATCA TTCCGCCCAC ATCGCTGGTG 
GTGCCGCTGC TGGGAAAGTT TGTGCTCTTC ACCATGATAC TGGATACCTT CAGCATCTGT 
GTGACTGTAT TGGTGCTGAA CATTCACTTC CGGTCGCCGC AAACGCACAC GATGGCTCCT 
TGGGTGCGGA CCGTGTTCAT CAACCAGCTG CCCCGCTTCC TGGTCATGCG ACGTCCGCTC 
TATCCGATCA GCGAGATGAT CAAATCCTCG CGCCGCCTAA TGGTGCGCAC CTGCAATGGA 
CTCGAACTGA GGGATCAAAT ACCACCGCTG CCACCGCCGG TGGCCTTGTC TCGTATGCAC 
CACAGCCCGA AGACGACATC GCGTAGCACC TCGCCGCATC TGCAGCACCG CGTGCAAACC 
TACAACCTGA TGGACGAGTG CGGCATGGGC ATGGGCGGCG GTGCCTCTAC CATTACCGGC 
CATTTGAGCT CCCTGTATCC GCCGACGATG TCGACGGCCA ACCAACAGAC CTCGACCACA 
TCATCGCTGA TCGATGCCCA GTACCACAAT CAGTACCAGC AGACGGGCGG TTCGCTGCTG 
GACCATCCAC TTACGCCCAC CAAGTTCCGC CAAATGACTT CGACCTCCAG CCAGACGGGT 
CAGAATGGCA GCGGGAACGG TAACGGTAAC GGTGGCTACG GCGGGCATGG CCAGAGCGGT 
GGCCATCATC TGTATCGACA GCAGTCGAGC TGCTCGGCGA ATTTCTCGCC GCTGCCGCAG 
CACGCCCACC AGGCGCACGC CTCCACCACC ACCAGCGATC TGGGCATGGC CAATCCGAAT 
GTGATAAAAT CGACGACGAC GGTTAACTCG GTGGCCACCG CCTCGACGCT GGCGGACTCC 
TGCTGCAGCG GCACCATTCA GATCCACCAG GGCATGGGCG CCGGAGCCGC TTGCCAGTCG 
TCGGAGCTGC TGCACCATCA GCAGTTGCAC CACCAGCAGA TGCATCACCA GCAGCAACAG 
CAGCAGCAGC AGCAGCATCA CCACCACCAG CGACCCACCA CCTCGGCAGC GGCGGCAGCA 
GCAGCGGCAG CCGCCGGCGG ACCCAGCACC TCGGCAGCGG CAGCTGCAGC AGCGGCGGCA 
GCGGCGGCCG GAACGCCACC CCCACCGCCT CCGCCGGCGG GCGTCTGTGA TATTCTGCCC 
GCCTGCCAGC GCGAGGGCGG GCCGCACTGC TGTTCGGGGC GTGGCAATGT GCGCCAACGG 
TGGCACACCT GTCCGGAACT CCACAAGGCC ATGGACGGGG TCACCTACAT TGCGGATCAG 
ACGCGCAAGG AAGAGGAGAG CACAAGGGTG AAAGAGGACT GGAAGTACGT GGCCATGGTG 
CTCGATCGCC TGTTCCTGTG GATCTTCACA ATAGCCGTGG TCGTCGGCAC CGCTGGCATT 
ATCCTGCAGG CTCCGACGCT CTACGACACC CGGATGCCCA TCGATATAAA GCTATCGGAG 
ATCGCCTCGA CGACGGCCAA GCCGAATGTG GCCAGGCCTG TGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525079.3
CDS	1362..3749
Translation	MKWFQVTIDMILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMPIDIKLSEIASTTAKPNVARPVL

Target ORF information:

RefSeq Version	NM_080340.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha3, transcript variant B (nAChRalpha3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080340.4

ORF Insert Sequence:

ATGAAGTGGT TTCAAGTGAC CATAGATATG ATTCTTGTGC TTTCATTTAT ATCAAGCAGC 
ACAGCGAATC CGGATGCGAA ACGATTGTAC GACGATTTGC TGAGCAACTA CAACAAACTG 
GTACGACCGG TGGTCAATGT GACGGACGCG CTCACCGTTC GCATCAAGCT GAAGCTATCG 
CAACTGATCG ATGTCAATCT GAAGAACCAG ATAATGACCA CCAATCTGTG GGTGGAGCAG 
TCCTGGTACG ACTACAAGCT CAAGTGGGAG CCCAAGGAGT ATGGCGGCGT CGAGATGCTT 
CACGTGCCCT CCGATCACAT CTGGCGTCCG GACATCGTTC TCTACAACAA CGCCGATGGC 
AATTTCGAGG TGACGCTGGC CACCAAGGCC ACGTTGAATT ACACAGGACG CGTCGAGTGG 
CGACCGCCGG CGATTTACAA GAGCTCCTGC GAAATCGACG TGGAGTACTT TCCCTTCGAC 
GAGCAGACCT GCGTCATGAA GTTCGGCAGC TGGACATACG ATGGATTTCA GGTGGATCTG 
CGGCACATCG ACGAGCTGAA CGGCACCAAT GTCGTGGAGG TGGGCGTGGA TCTGTCCGAG 
TTCTATACGT CCGTCGAATG GGATATCCTC GAAGTTCCTG CAGTGCGAAA CGAGAAGTTC 
TACACGTGCT GCGATGAGCC ATATCTGGAC ATCACCTTCA ACATAACGAT GCGTCGGAAG 
ACTCTCTTCT ATACCGTCAA CCTGATCATT CCCTGCATGG GCATCAGTTT TCTCACCATC 
CTCGTATTCT ACCTGCCATC GGATAGTGGC GAGAAGGTCT CATTAAGCAT ATCCATTCTG 
CTGTCGCTCA CTGTGTTCTT CCTGCTGCTG GCGGAAATCA TTCCGCCCAC ATCGCTGGTG 
GTGCCGCTGC TGGGAAAGTT TGTGCTCTTC ACCATGATAC TGGATACCTT CAGCATCTGT 
GTGACTGTAT TGGTGCTGAA CATTCACTTC CGGTCGCCGC AAACGCACAC GATGGCTCCT 
TGGGTGCGGA CCGTGTTCAT CAACCAGCTG CCCCGCTTCC TGGTCATGCG ACGTCCGCTC 
TATCCGATCA GCGAGATGAT CAAATCCTCG CGCCGCCTAA TGGTGCGCAC CTGCAATGGA 
CTCGAACTGA GGGATCAAAT ACCACCGCTG CCACCGCCGG TGGCCTTGTC TCGTATGCAC 
CACAGCCCGA AGACGACATC GCGTAGCACC TCGCCGCATC TGCAGCACCG CGTGCAAACC 
TACAACCTGA TGGACGAGTG CGGCATGGGC ATGGGCGGCG GTGCCTCTAC CATTACCGGC 
CATTTGAGCT CCCTGTATCC GCCGACGATG TCGACGGCCA ACCAACAGAC CTCGACCACA 
TCATCGCTGA TCGATGCCCA GTACCACAAT CAGTACCAGC AGACGGGCGG TTCGCTGCTG 
GACCATCCAC TTACGCCCAC CAAGTTCCGC CAAATGACTT CGACCTCCAG CCAGACGGGT 
CAGAATGGCA GCGGGAACGG TAACGGTAAC GGTGGCTACG GCGGGCATGG CCAGAGCGGT 
GGCCATCATC TGTATCGACA GCAGTCGAGC TGCTCGGCGA ATTTCTCGCC GCTGCCGCAG 
CACGCCCACC AGGCGCACGC CTCCACCACC ACCAGCGATC TGGGCATGGC CAATCCGAAT 
GTGATAAAAT CGACGACGAC GGTTAACTCG GTGGCCACCG CCTCGACGCT GGCGGACTCC 
TGCTGCAGCG GCACCATTCA GATCCACCAG GGCATGGGCG CCGGAGCCGC TTGCCAGTCG 
TCGGAGCTGC TGCACCATCA GCAGTTGCAC CACCAGCAGA TGCATCACCA GCAGCAACAG 
CAGCAGCAGC AGCAGCATCA CCACCACCAG CGACCCACCA CCTCGGCAGC GGCGGCAGCA 
GCAGCGGCAG CCGCCGGCGG ACCCAGCACC TCGGCAGCGG CAGCTGCAGC AGCGGCGGCA 
GCGGCGGCCG GAACGCCACC CCCACCGCCT CCGCCGGCGG GCGTCTGTGA TATTCTGCCC 
GCCTGCCAGC GCGAGGGCGG GCCGCACTGC TGTTCGGGGC GTGGCAATGT GCGCCAACGG 
TGGCACACCT GTCCGGAACT CCACAAGGCC ATGGACGGGG TCACCTACAT TGCGGATCAG 
ACGCGCAAGG AAGAGGAGAG CACAAGGGTG AAAGAGGACT GGAAGTACGT GGCCATGGTG 
CTCGATCGCC TGTTCCTGTG GATCTTCACA ATAGCCGTGG TCGTCGGCAC CGCTGGCATT 
ATCCTGCAGG CTCCGACGCT CTACGACACC CGGATGCCCA TCGATATAAA GCTATCGGAG 
ATCGCCTCGA CGACGGCCAA GCCGAATGTG GCCAGGCCTG TGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nAChRalpha3 ( NM_001298086.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285015.1 Drosophila melanogaster nicotinic acetylcholine receptor alpha3, transcript variant C (nAChRalpha3), mRNA.

Price & Availability↑

CloneID	OFa01106
Clone ID Related Accession (Same CDS sequence)	NM_080340.4 , NM_001298086.1
Accession Version	NM_001298086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nicotinic acetylcholine receptor alpha3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCAAGTGAC CATAGATATG ATTCTTGTGC TTTCATTTAT ATCAAGCAGC 
ACAGCGAATC CGGATGCGAA ACGATTGTAC GACGATTTGC TGAGCAACTA CAACAAACTG 
GTACGACCGG TGGTCAATGT GACGGACGCG CTCACCGTTC GCATCAAGCT GAAGCTATCG 
CAACTGATCG ATGTCAATCT GAAGAACCAG ATAATGACCA CCAATCTGTG GGTGGAGCAG 
TCCTGGTACG ACTACAAGCT CAAGTGGGAG CCCAAGGAGT ATGGCGGCGT CGAGATGCTT 
CACGTGCCCT CCGATCACAT CTGGCGTCCG GACATCGTTC TCTACAACAA CGCCGATGGC 
AATTTCGAGG TGACGCTGGC CACCAAGGCC ACGTTGAATT ACACAGGACG CGTCGAGTGG 
CGACCGCCGG CGATTTACAA GAGCTCCTGC GAAATCGACG TGGAGTACTT TCCCTTCGAC 
GAGCAGACCT GCGTCATGAA GTTCGGCAGC TGGACATACG ATGGATTTCA GGTGGATCTG 
CGGCACATCG ACGAGCTGAA CGGCACCAAT GTCGTGGAGG TGGGCGTGGA TCTGTCCGAG 
TTCTATACGT CCGTCGAATG GGATATCCTC GAAGTTCCTG CAGTGCGAAA CGAGAAGTTC 
TACACGTGCT GCGATGAGCC ATATCTGGAC ATCACCTTCA ACATAACGAT GCGTCGGAAG 
ACTCTCTTCT ATACCGTCAA CCTGATCATT CCCTGCATGG GCATCAGTTT TCTCACCATC 
CTCGTATTCT ACCTGCCATC GGATAGTGGC GAGAAGGTCT CATTAAGCAT ATCCATTCTG 
CTGTCGCTCA CTGTGTTCTT CCTGCTGCTG GCGGAAATCA TTCCGCCCAC ATCGCTGGTG 
GTGCCGCTGC TGGGAAAGTT TGTGCTCTTC ACCATGATAC TGGATACCTT CAGCATCTGT 
GTGACTGTAT TGGTGCTGAA CATTCACTTC CGGTCGCCGC AAACGCACAC GATGGCTCCT 
TGGGTGCGGA CCGTGTTCAT CAACCAGCTG CCCCGCTTCC TGGTCATGCG ACGTCCGCTC 
TATCCGATCA GCGAGATGAT CAAATCCTCG CGCCGCCTAA TGGTGCGCAC CTGCAATGGA 
CTCGAACTGA GGGATCAAAT ACCACCGCTG CCACCGCCGG TGGCCTTGTC TCGTATGCAC 
CACAGCCCGA AGACGACATC GCGTAGCACC TCGCCGCATC TGCAGCACCG CGTGCAAACC 
TACAACCTGA TGGACGAGTG CGGCATGGGC ATGGGCGGCG GTGCCTCTAC CATTACCGGC 
CATTTGAGCT CCCTGTATCC GCCGACGATG TCGACGGCCA ACCAACAGAC CTCGACCACA 
TCATCGCTGA TCGATGCCCA GTACCACAAT CAGTACCAGC AGACGGGCGG TTCGCTGCTG 
GACCATCCAC TTACGCCCAC CAAGTTCCGC CAAATGACTT CGACCTCCAG CCAGACGGGT 
CAGAATGGCA GCGGGAACGG TAACGGTAAC GGTGGCTACG GCGGGCATGG CCAGAGCGGT 
GGCCATCATC TGTATCGACA GCAGTCGAGC TGCTCGGCGA ATTTCTCGCC GCTGCCGCAG 
CACGCCCACC AGGCGCACGC CTCCACCACC ACCAGCGATC TGGGCATGGC CAATCCGAAT 
GTGATAAAAT CGACGACGAC GGTTAACTCG GTGGCCACCG CCTCGACGCT GGCGGACTCC 
TGCTGCAGCG GCACCATTCA GATCCACCAG GGCATGGGCG CCGGAGCCGC TTGCCAGTCG 
TCGGAGCTGC TGCACCATCA GCAGTTGCAC CACCAGCAGA TGCATCACCA GCAGCAACAG 
CAGCAGCAGC AGCAGCATCA CCACCACCAG CGACCCACCA CCTCGGCAGC GGCGGCAGCA 
GCAGCGGCAG CCGCCGGCGG ACCCAGCACC TCGGCAGCGG CAGCTGCAGC AGCGGCGGCA 
GCGGCGGCCG GAACGCCACC CCCACCGCCT CCGCCGGCGG GCGTCTGTGA TATTCTGCCC 
GCCTGCCAGC GCGAGGGCGG GCCGCACTGC TGTTCGGGGC GTGGCAATGT GCGCCAACGG 
TGGCACACCT GTCCGGAACT CCACAAGGCC ATGGACGGGG TCACCTACAT TGCGGATCAG 
ACGCGCAAGG AAGAGGAGAG CACAAGGGTG AAAGAGGACT GGAAGTACGT GGCCATGGTG 
CTCGATCGCC TGTTCCTGTG GATCTTCACA ATAGCCGTGG TCGTCGGCAC CGCTGGCATT 
ATCCTGCAGG CTCCGACGCT CTACGACACC CGGATGCCCA TCGATATAAA GCTATCGGAG 
ATCGCCTCGA CGACGGCCAA GCCGAATGTG GCCAGGCCTG TGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285015.1
CDS	1362..3749
Translation	MKWFQVTIDMILVLSFISSSTANPDAKRLYDDLLSNYNKLVRPVVNVTDALTVRIKLKLSQLIDVNLKNQIMTTNLWVEQSWYDYKLKWEPKEYGGVEMLHVPSDHIWRPDIVLYNNADGNFEVTLATKATLNYTGRVEWRPPAIYKSSCEIDVEYFPFDEQTCVMKFGSWTYDGFQVDLRHIDELNGTNVVEVGVDLSEFYTSVEWDILEVPAVRNEKFYTCCDEPYLDITFNITMRRKTLFYTVNLIIPCMGISFLTILVFYLPSDSGEKVSLSISILLSLTVFFLLLAEIIPPTSLVVPLLGKFVLFTMILDTFSICVTVLVLNIHFRSPQTHTMAPWVRTVFINQLPRFLVMRRPLYPISEMIKSSRRLMVRTCNGLELRDQIPPLPPPVALSRMHHSPKTTSRSTSPHLQHRVQTYNLMDECGMGMGGGASTITGHLSSLYPPTMSTANQQTSTTSSLIDAQYHNQYQQTGGSLLDHPLTPTKFRQMTSTSSQTGQNGSGNGNGNGGYGGHGQSGGHHLYRQQSSCSANFSPLPQHAHQAHASTTTSDLGMANPNVIKSTTTVNSVATASTLADSCCSGTIQIHQGMGAGAACQSSELLHHQQLHHQQMHHQQQQQQQQQHHHHQRPTTSAAAAAAAAAAGGPSTSAAAAAAAAAAAAGTPPPPPPPAGVCDILPACQREGGPHCCSGRGNVRQRWHTCPELHKAMDGVTYIADQTRKEEESTRVKEDWKYVAMVLDRLFLWIFTIAVVVGTAGIILQAPTLYDTRMPIDIKLSEIASTTAKPNVARPVL

Target ORF information:

RefSeq Version	NM_001298086.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nicotinic acetylcholine receptor alpha3, transcript variant C (nAChRalpha3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298086.1

ORF Insert Sequence:

ATGAAGTGGT TTCAAGTGAC CATAGATATG ATTCTTGTGC TTTCATTTAT ATCAAGCAGC 
ACAGCGAATC CGGATGCGAA ACGATTGTAC GACGATTTGC TGAGCAACTA CAACAAACTG 
GTACGACCGG TGGTCAATGT GACGGACGCG CTCACCGTTC GCATCAAGCT GAAGCTATCG 
CAACTGATCG ATGTCAATCT GAAGAACCAG ATAATGACCA CCAATCTGTG GGTGGAGCAG 
TCCTGGTACG ACTACAAGCT CAAGTGGGAG CCCAAGGAGT ATGGCGGCGT CGAGATGCTT 
CACGTGCCCT CCGATCACAT CTGGCGTCCG GACATCGTTC TCTACAACAA CGCCGATGGC 
AATTTCGAGG TGACGCTGGC CACCAAGGCC ACGTTGAATT ACACAGGACG CGTCGAGTGG 
CGACCGCCGG CGATTTACAA GAGCTCCTGC GAAATCGACG TGGAGTACTT TCCCTTCGAC 
GAGCAGACCT GCGTCATGAA GTTCGGCAGC TGGACATACG ATGGATTTCA GGTGGATCTG 
CGGCACATCG ACGAGCTGAA CGGCACCAAT GTCGTGGAGG TGGGCGTGGA TCTGTCCGAG 
TTCTATACGT CCGTCGAATG GGATATCCTC GAAGTTCCTG CAGTGCGAAA CGAGAAGTTC 
TACACGTGCT GCGATGAGCC ATATCTGGAC ATCACCTTCA ACATAACGAT GCGTCGGAAG 
ACTCTCTTCT ATACCGTCAA CCTGATCATT CCCTGCATGG GCATCAGTTT TCTCACCATC 
CTCGTATTCT ACCTGCCATC GGATAGTGGC GAGAAGGTCT CATTAAGCAT ATCCATTCTG 
CTGTCGCTCA CTGTGTTCTT CCTGCTGCTG GCGGAAATCA TTCCGCCCAC ATCGCTGGTG 
GTGCCGCTGC TGGGAAAGTT TGTGCTCTTC ACCATGATAC TGGATACCTT CAGCATCTGT 
GTGACTGTAT TGGTGCTGAA CATTCACTTC CGGTCGCCGC AAACGCACAC GATGGCTCCT 
TGGGTGCGGA CCGTGTTCAT CAACCAGCTG CCCCGCTTCC TGGTCATGCG ACGTCCGCTC 
TATCCGATCA GCGAGATGAT CAAATCCTCG CGCCGCCTAA TGGTGCGCAC CTGCAATGGA 
CTCGAACTGA GGGATCAAAT ACCACCGCTG CCACCGCCGG TGGCCTTGTC TCGTATGCAC 
CACAGCCCGA AGACGACATC GCGTAGCACC TCGCCGCATC TGCAGCACCG CGTGCAAACC 
TACAACCTGA TGGACGAGTG CGGCATGGGC ATGGGCGGCG GTGCCTCTAC CATTACCGGC 
CATTTGAGCT CCCTGTATCC GCCGACGATG TCGACGGCCA ACCAACAGAC CTCGACCACA 
TCATCGCTGA TCGATGCCCA GTACCACAAT CAGTACCAGC AGACGGGCGG TTCGCTGCTG 
GACCATCCAC TTACGCCCAC CAAGTTCCGC CAAATGACTT CGACCTCCAG CCAGACGGGT 
CAGAATGGCA GCGGGAACGG TAACGGTAAC GGTGGCTACG GCGGGCATGG CCAGAGCGGT 
GGCCATCATC TGTATCGACA GCAGTCGAGC TGCTCGGCGA ATTTCTCGCC GCTGCCGCAG 
CACGCCCACC AGGCGCACGC CTCCACCACC ACCAGCGATC TGGGCATGGC CAATCCGAAT 
GTGATAAAAT CGACGACGAC GGTTAACTCG GTGGCCACCG CCTCGACGCT GGCGGACTCC 
TGCTGCAGCG GCACCATTCA GATCCACCAG GGCATGGGCG CCGGAGCCGC TTGCCAGTCG 
TCGGAGCTGC TGCACCATCA GCAGTTGCAC CACCAGCAGA TGCATCACCA GCAGCAACAG 
CAGCAGCAGC AGCAGCATCA CCACCACCAG CGACCCACCA CCTCGGCAGC GGCGGCAGCA 
GCAGCGGCAG CCGCCGGCGG ACCCAGCACC TCGGCAGCGG CAGCTGCAGC AGCGGCGGCA 
GCGGCGGCCG GAACGCCACC CCCACCGCCT CCGCCGGCGG GCGTCTGTGA TATTCTGCCC 
GCCTGCCAGC GCGAGGGCGG GCCGCACTGC TGTTCGGGGC GTGGCAATGT GCGCCAACGG 
TGGCACACCT GTCCGGAACT CCACAAGGCC ATGGACGGGG TCACCTACAT TGCGGATCAG 
ACGCGCAAGG AAGAGGAGAG CACAAGGGTG AAAGAGGACT GGAAGTACGT GGCCATGGTG 
CTCGATCGCC TGTTCCTGTG GATCTTCACA ATAGCCGTGG TCGTCGGCAC CGCTGGCATT 
ATCCTGCAGG CTCCGACGCT CTACGACACC CGGATGCCCA TCGATATAAA GCTATCGGAG 
ATCGCCTCGA CGACGGCCAA GCCGAATGTG GCCAGGCCTG TGTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nAChRalpha3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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