UbcE2H cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	UbcE2H
Entrez Gene ID	31728
Full Name	Ubiquitin conjugating enzyme E2H
Synonyms	CG2257,Dmel\CG2257,Ubc-E2H,Ubc-E2Hs,UbcDE2H,dUbc-E2H,ubcE2h
General protein information	Preferred Names Ubiquitin conjugating enzyme E2H Names CG2257 gene product from transcript CG2257-RA CG2257-PA CG2257-PB CG2257-PC Ubc-E2H UbcE2H-PA UbcE2H-PB UbcE2H-PC ubiquitin conjugating enzyme E2H
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D6-7D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_167145.2	NP_727233.1	ubiquitin conjugating enzyme E2H, isoform A
NM_132210.3	NP_572438.1	ubiquitin conjugating enzyme E2H, isoform B
NM_167146.2	NP_727234.1	ubiquitin conjugating enzyme E2H, isoform C

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2H	NP_003335.1
Pan troglodytes (chimpanzee)	UBE2H	XP_001144807.2
Gallus gallus (chicken)	UBE2H	NP_001025818.1
Danio rerio (zebrafish)	ube2h	NP_958897.1
Drosophila melanogaster (fruit fly)	Ubc-E2H	NP_727234.1
Caenorhabditis elegans (roundworm)	ubc-8	NP_500245.2
Bos taurus (cattle)	UBE2H	NP_001032703.1
Rattus norvegicus (Norway rat)	Ube2h	NP_001165598.1
Schizosaccharomyces pombe (fission yeast)	ubc8	NP_596617.1
Neurospora crassa	NCU10046	XP_957953.1
Arabidopsis thaliana (thale cress)	UBC5	NP_564817.2
Arabidopsis thaliana (thale cress)	UBC4	NP_568589.1
Magnaporthe oryzae (rice blast fungus)	MGG_00600	XP_003718439.1
Xenopus tropicalis (tropical clawed frog)	ube2h	NP_001004909.1
	UBE2H	XP_001093822.1
Canis lupus familiaris (dog)	UBE2H	XP_857419.1
Eremothecium gossypii	AGOS_AEL045W	NP_984816.1
Arabidopsis thaliana (thale cress)	UBC6	NP_566062.1
Mus musculus (house mouse)	Ube2h	NP_033485.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001068	XP_318292.1
Saccharomyces cerevisiae (baker's yeast)	UBC8	NP_010904.2
Kluyveromyces lactis	KLLA0A11198g	XP_451487.1
Oryza sativa (rice)	Os10g0447100	NP_001064719.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following UbcE2H gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UbcE2H cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01074	NM_167146.2 Latest version!	Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant C (UbcE2H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa01074	NM_167145.2 Latest version!	Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant A (UbcE2H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa01074	NM_132210.3 Latest version!	Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant B (UbcE2H), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UbcE2H ( NM_167145.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727233.1 Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant A (UbcE2H), mRNA.

Price & Availability↑

CloneID	OFa01074
Clone ID Related Accession (Same CDS sequence)	NM_167145.2 , NM_132210.3 , NM_167146.2
Accession Version	NM_167145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin conjugating enzyme E2H, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167145.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTCGC CCAGTGCCGG AAAGCGACGC ATGGACAACG ATGTGATCAA GCTGATCGAA 
TCAAAGCACG AGGTGACCAT CCTGGGAGGC CTCAACGAGT TCCACGTCAA GTTCTTCGGT 
CCAACGGAGA CGCCCTACGA GGGCGGCGTG TGGAAGGTGC GCGTCTACCT GCCCGACAAC 
TATCCCTTCA AGTCACCGAG CATTGGTTTT GTGAACAAGA TCTACCATCC GAACATTGAC 
GAATCCTCGG GTACGGTGTG CCTGGATGTT ATCAATCAGG CCTGGACGGC GTTGTACGAT 
CTATCGAACA TATTTGAATC GTTCTTGCCG CAGCTGCTCA CATATCCCAA TCCGGTCGAT 
CCGCTCAATC GGGATGCGGC CGCATTGTAC CTGCACGAGC CGGAGGAGTA TCATCGCAAG 
GTGGCCGACT ATGTGCAGCG CTATGCCACC GAGGACGCGT TGCGGGCGGC GCAGCAGGAG 
CGGGAGAGCA GCGACAGCGA GTCGAGCATG TCCGACTACA GTGAGGATGA GGCCAGGGAC 
ATGGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727233.1
CDS	578..1129
Translation	MSSPSAGKRRMDNDVIKLIESKHEVTILGGLNEFHVKFFGPTETPYEGGVWKVRVYLPDNYPFKSPSIGFVNKIYHPNIDESSGTVCLDVINQAWTALYDLSNIFESFLPQLLTYPNPVDPLNRDAAALYLHEPEEYHRKVADYVQRYATEDALRAAQQERESSDSESSMSDYSEDEARDMEL

Target ORF information:

RefSeq Version	NM_167145.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant A (UbcE2H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167145.2

ORF Insert Sequence:

ATGTCCTCGC CCAGTGCCGG AAAGCGACGC ATGGACAACG ATGTGATCAA GCTGATCGAA 
TCAAAGCACG AGGTGACCAT CCTGGGAGGC CTCAACGAGT TCCACGTCAA GTTCTTCGGT 
CCAACGGAGA CGCCCTACGA GGGCGGCGTG TGGAAGGTGC GCGTCTACCT GCCCGACAAC 
TATCCCTTCA AGTCACCGAG CATTGGTTTT GTGAACAAGA TCTACCATCC GAACATTGAC 
GAATCCTCGG GTACGGTGTG CCTGGATGTT ATCAATCAGG CCTGGACGGC GTTGTACGAT 
CTATCGAACA TATTTGAATC GTTCTTGCCG CAGCTGCTCA CATATCCCAA TCCGGTCGAT 
CCGCTCAATC GGGATGCGGC CGCATTGTAC CTGCACGAGC CGGAGGAGTA TCATCGCAAG 
GTGGCCGACT ATGTGCAGCG CTATGCCACC GAGGACGCGT TGCGGGCGGC GCAGCAGGAG 
CGGGAGAGCA GCGACAGCGA GTCGAGCATG TCCGACTACA GTGAGGATGA GGCCAGGGAC 
ATGGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UbcE2H ( NM_132210.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572438.1 Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant B (UbcE2H), mRNA.

Price & Availability↑

CloneID	OFa01074
Clone ID Related Accession (Same CDS sequence)	NM_167145.2 , NM_132210.3 , NM_167146.2
Accession Version	NM_132210.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin conjugating enzyme E2H, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132210.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTCGC CCAGTGCCGG AAAGCGACGC ATGGACAACG ATGTGATCAA GCTGATCGAA 
TCAAAGCACG AGGTGACCAT CCTGGGAGGC CTCAACGAGT TCCACGTCAA GTTCTTCGGT 
CCAACGGAGA CGCCCTACGA GGGCGGCGTG TGGAAGGTGC GCGTCTACCT GCCCGACAAC 
TATCCCTTCA AGTCACCGAG CATTGGTTTT GTGAACAAGA TCTACCATCC GAACATTGAC 
GAATCCTCGG GTACGGTGTG CCTGGATGTT ATCAATCAGG CCTGGACGGC GTTGTACGAT 
CTATCGAACA TATTTGAATC GTTCTTGCCG CAGCTGCTCA CATATCCCAA TCCGGTCGAT 
CCGCTCAATC GGGATGCGGC CGCATTGTAC CTGCACGAGC CGGAGGAGTA TCATCGCAAG 
GTGGCCGACT ATGTGCAGCG CTATGCCACC GAGGACGCGT TGCGGGCGGC GCAGCAGGAG 
CGGGAGAGCA GCGACAGCGA GTCGAGCATG TCCGACTACA GTGAGGATGA GGCCAGGGAC 
ATGGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572438.1
CDS	441..992
Translation	MSSPSAGKRRMDNDVIKLIESKHEVTILGGLNEFHVKFFGPTETPYEGGVWKVRVYLPDNYPFKSPSIGFVNKIYHPNIDESSGTVCLDVINQAWTALYDLSNIFESFLPQLLTYPNPVDPLNRDAAALYLHEPEEYHRKVADYVQRYATEDALRAAQQERESSDSESSMSDYSEDEARDMEL

Target ORF information:

RefSeq Version	NM_132210.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant B (UbcE2H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132210.3

ORF Insert Sequence:

ATGTCCTCGC CCAGTGCCGG AAAGCGACGC ATGGACAACG ATGTGATCAA GCTGATCGAA 
TCAAAGCACG AGGTGACCAT CCTGGGAGGC CTCAACGAGT TCCACGTCAA GTTCTTCGGT 
CCAACGGAGA CGCCCTACGA GGGCGGCGTG TGGAAGGTGC GCGTCTACCT GCCCGACAAC 
TATCCCTTCA AGTCACCGAG CATTGGTTTT GTGAACAAGA TCTACCATCC GAACATTGAC 
GAATCCTCGG GTACGGTGTG CCTGGATGTT ATCAATCAGG CCTGGACGGC GTTGTACGAT 
CTATCGAACA TATTTGAATC GTTCTTGCCG CAGCTGCTCA CATATCCCAA TCCGGTCGAT 
CCGCTCAATC GGGATGCGGC CGCATTGTAC CTGCACGAGC CGGAGGAGTA TCATCGCAAG 
GTGGCCGACT ATGTGCAGCG CTATGCCACC GAGGACGCGT TGCGGGCGGC GCAGCAGGAG 
CGGGAGAGCA GCGACAGCGA GTCGAGCATG TCCGACTACA GTGAGGATGA GGCCAGGGAC 
ATGGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UbcE2H ( NM_167146.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727234.1 Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant C (UbcE2H), mRNA.

Price & Availability↑

CloneID	OFa01074
Clone ID Related Accession (Same CDS sequence)	NM_167145.2 , NM_132210.3 , NM_167146.2
Accession Version	NM_167146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ubiquitin conjugating enzyme E2H, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167146.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCTCGC CCAGTGCCGG AAAGCGACGC ATGGACAACG ATGTGATCAA GCTGATCGAA 
TCAAAGCACG AGGTGACCAT CCTGGGAGGC CTCAACGAGT TCCACGTCAA GTTCTTCGGT 
CCAACGGAGA CGCCCTACGA GGGCGGCGTG TGGAAGGTGC GCGTCTACCT GCCCGACAAC 
TATCCCTTCA AGTCACCGAG CATTGGTTTT GTGAACAAGA TCTACCATCC GAACATTGAC 
GAATCCTCGG GTACGGTGTG CCTGGATGTT ATCAATCAGG CCTGGACGGC GTTGTACGAT 
CTATCGAACA TATTTGAATC GTTCTTGCCG CAGCTGCTCA CATATCCCAA TCCGGTCGAT 
CCGCTCAATC GGGATGCGGC CGCATTGTAC CTGCACGAGC CGGAGGAGTA TCATCGCAAG 
GTGGCCGACT ATGTGCAGCG CTATGCCACC GAGGACGCGT TGCGGGCGGC GCAGCAGGAG 
CGGGAGAGCA GCGACAGCGA GTCGAGCATG TCCGACTACA GTGAGGATGA GGCCAGGGAC 
ATGGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727234.1
CDS	477..1028
Translation	MSSPSAGKRRMDNDVIKLIESKHEVTILGGLNEFHVKFFGPTETPYEGGVWKVRVYLPDNYPFKSPSIGFVNKIYHPNIDESSGTVCLDVINQAWTALYDLSNIFESFLPQLLTYPNPVDPLNRDAAALYLHEPEEYHRKVADYVQRYATEDALRAAQQERESSDSESSMSDYSEDEARDMEL

Target ORF information:

RefSeq Version	NM_167146.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ubiquitin conjugating enzyme E2H, transcript variant C (UbcE2H), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167146.2

ORF Insert Sequence:

ATGTCCTCGC CCAGTGCCGG AAAGCGACGC ATGGACAACG ATGTGATCAA GCTGATCGAA 
TCAAAGCACG AGGTGACCAT CCTGGGAGGC CTCAACGAGT TCCACGTCAA GTTCTTCGGT 
CCAACGGAGA CGCCCTACGA GGGCGGCGTG TGGAAGGTGC GCGTCTACCT GCCCGACAAC 
TATCCCTTCA AGTCACCGAG CATTGGTTTT GTGAACAAGA TCTACCATCC GAACATTGAC 
GAATCCTCGG GTACGGTGTG CCTGGATGTT ATCAATCAGG CCTGGACGGC GTTGTACGAT 
CTATCGAACA TATTTGAATC GTTCTTGCCG CAGCTGCTCA CATATCCCAA TCCGGTCGAT 
CCGCTCAATC GGGATGCGGC CGCATTGTAC CTGCACGAGC CGGAGGAGTA TCATCGCAAG 
GTGGCCGACT ATGTGCAGCG CTATGCCACC GAGGACGCGT TGCGGGCGGC GCAGCAGGAG 
CGGGAGAGCA GCGACAGCGA GTCGAGCATG TCCGACTACA GTGAGGATGA GGCCAGGGAC 
ATGGAATTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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UbcE2H cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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