Upf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Upf2
Entrez Gene ID	31724
Full Name	CG2253 gene product from transcript CG2253-RA
Synonyms	CG2253,Dmel\CG2253,UPF2,smg-3,upf2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D5-7D5

mRNA and Protein(s)

mRNA	Protein	Name
NM_132206.3	NP_572434.1	Upf2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UPF2	XP_005214170.1
Homo sapiens (human)	UPF2	NP_542166.1
Rattus norvegicus (Norway rat)	Upf2	NP_001101891.1
Danio rerio (zebrafish)	upf2	XP_697460.6
Drosophila melanogaster (fruit fly)	Upf2	NP_572434.1
Neurospora crassa	NCU05267	XP_961757.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000337	XP_310780.5
Saccharomyces cerevisiae (baker's yeast)	NMD2	NP_011944.2
Eremothecium gossypii	AGOS_AGR364C	NP_987030.2
Pan troglodytes (chimpanzee)	UPF2	XP_507656.3
	UPF2	XP_002805601.1
Gallus gallus (chicken)	UPF2	XP_004937542.1
Kluyveromyces lactis	KLLA0D13156g	XP_453649.1
Magnaporthe oryzae (rice blast fungus)	MGG_06063	XP_003711916.1
Arabidopsis thaliana (thale cress)	AT2G39260	NP_181459.4
Canis lupus familiaris (dog)	UPF2	XP_005617199.1
Mus musculus (house mouse)	Upf2	NP_001074601.1
Schizosaccharomyces pombe (fission yeast)	upf2	NP_593784.1
Xenopus tropicalis (tropical clawed frog)	upf2	NP_001121475.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the lethality of upf1 and upf2 mutants is not due to disrupting their roles during Nonsense-mediated RNA decay (NMD) -independent processes, but to their function in the degradation of specific mRNAs by the NMD pathway.
Title: Drosophila Upf1 and Upf2 loss of function inhibits cell growth and causes animal death in a Upf3-independent manner.

The following Upf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Upf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01070	NM_132206.3 Latest version!	Drosophila melanogaster Upf2 (Upf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Upf2 ( NM_132206.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572434.1 Drosophila melanogaster Upf2 (Upf2), mRNA.

Price & Availability↑

CloneID	OFa01070
Clone ID Related Accession (Same CDS sequence)	NM_132206.3
Accession Version	NM_132206.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Upf2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132206.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAGCCA ACGATTCTGC AACGACTGAC AGCGAGGTGT CCACACCGCC GTCGTCGAGG 
AAAGATTTGG CAGATGGTGT TGCCGAGGGT GACAACGACA ACGACATCGA TACCGGCGAC 
ACGGACGCAA ATGCGGATGA AGATGTGGAT GCGGCTGCTG CCATTGCCCT GGAGGCCGAA 
GAGCGCGAGG AGCTGCAACA GTTCATAAGC GAGCTGCGCG AGAAGATCGA GAGCAAGCGA 
CAGCTGCGCC TCCAAAACTC CACAATCGAG CTGCCCGGTG AGGAGTACTT CGCCAAACTG 
GACTCCAATC TCAAAAAGAA CACGGCATTC GTCAAGAAAC TGAAACTATT CACGGCCACA 
CAGCTGGACG GACTATTGCG TGAGATATCT GCTCTAAACC TGAGCAAATA CATCTCGGAA 
ATATGCGCCG CGCTGGTCGA GGCCAAACTG AAAATGACTG ATGTGCCGGC GGTGGTGACG 
CTGGCCTCTC GGCTGCACTG CACATATGCC GACTTCGATG TCCATTTTCT GGAGGCCTGG 
CAAAAGGCAC TGAACATCAA GGCCACCGAG AAGATTGGCA ATCCGAGCAA ACTGCGCGTG 
GATCTTCGCC TGTTCGCCGA ACTAGTCAGC TCTGGTGTGA TCCAAATGAA GCCCGGATTG 
GCCCAACTGG GTGTTGTTCT GGTGCATTTG ATTGCCCTGG ACAAGGATGA CCACAGCAAC 
TTCTCGATCA TCCTCTCATT CTGCCGGCAC TGCGGCGAGG AGTACGCCGG CCTAGTGCCG 
CAAAAGATGC AACAGTTGGC CACCAAATAC GGGGTGGAGG TGCCGAAATC CGACTTCCTG 
ACTGCCGACA AGCAGCTCAA TCTGCGCACC ATGCTTAAGG GTTACTTCAA GGCCCTGTGC 
AAGCATGTCC TGGCCGAGCA GGCCGAACTG ATGAACATGA CCAAGAACAT TCGGCGCACC 
ATGGAGTGCA AGGGTGAGAT ATCCACGGAG AAGCGCGAGA AGTGTGAACT GATGCAGGCC 
GGATTCGATA AGCTGCTGGC CTCCGCTCAA TCGCTGTCGG AACTGCTGGG TGAACCACTG 
CCCGAGCTGG CCAAGGAGTC TGAGGGCTGT AATCCGGGCA CAGTCATCGA CAACATGCTG 
GACAGTGCCT CCTTTGGTGT GCTGGATCCG TGGGGCGACG AGGAGACCCG AGCCTTCTAC 
ACGGATTTGC CCGATTTGAG ACAGTTTCTG CCAAACTTTT CGGCACCCAA AGTCGATTTG 
GAAACGTTAG AGGAGCCCAG TGAACTAACC GAAGAAGCGA TCGATGCCAA TCTAGATGCC 
GAAATGGATC TAGATGATCC ACCGTCCACC ACTTCGGATA CGACTCCGGA GAATCCAATC 
GAAGAGCAGC CAACGACGCC AGTTGCCGCT GCGGAGGATC TCAAGCCGCA GAAGATGGGT 
AACGCTCTAA TGGAACTGGG TCGCCAGCAG CAGCAGCAAA GTCAGCTCAA CCAGAATCCG 
ATCCAGATCC AAAACCAGAT GCGTCAGCAG TTCGATGGCT TCCTGGTCAA CCTATTCAAC 
TGTGTGAACA AGGAGCTGAT CGATTCGGCG GCCATCGAGT TCCTGCTTAA CTTCAATACC 
AAGCATCAAC GCAAGAAGCT GACGCGTACG ATATTCTCGG TGCAGCGTAC GCGTCTGGAT 
ATCCTGCCCT ATCTCTCCCG CTTCGTGGCC ATTGTGCATA TGTGCAATAC CGATGTGGCC 
ACCGATTTGG CCGAACTGCT GCGCAAGGAG TTCAAGTGGC ACATTCACAA GAAGAACCAG 
CTGAATATCG AGACGAAGCT TAAGATTGTG CGATTCATTG GTGAACTGGT TAAGTTTGGT 
TTGTTTAAAA AGTTCGATGC CTTAGGATGT CTGAAGATGC TGCTAAGGGA CTTTCAGCAT 
CATCAAATCG AGATGGCCTG TGCCTTTGTG GAAGTCACCG GCGTCTATCT GTACAATTGC 
CGGGATGCCC GTCTGCTGAT GAATGTGTTC CTGGACCAGA TGCTGCGCTT GAAAACAGCC 
ACGGCAATGG ACTCGCGACA TGCGGCGCAG ATTGAGAGCG TGTACTATTT GGTAAAGCCG 
CCGGAATCGT CCAAGCGAGA GCCCATGGTT CGGCCGGCAA TGCATGAGTA CATCCGTTAC 
TTGATCTTCG AGGAGCTTTG CAAACAGAAC GTTGATCGTT GTATCAAAAT GCTGCGCCGC 
ATCGATTGGC AGGATCCGGA GACAAATTGT TATGCCATTA AGTGCCTCAG CAAGGCGTAT 
CTGCTGCGCT TCCAGCTCAT CCGCTGTCTG GCCGATCTCG TATCCGGACT GAGCTCCTAT 
CAGCCACGAG CGGTTACCAT CGTGATCGAT AATGTGTTTG AGGATATACG CGCTGGCCTC 
GAGATTCATT CGCCACGCAT GGCACAGCGG CGCATCGCGA TGGCCAAGTA TCTGGGCGAG 
ATGTACAACT ACAAGCTGGT GGAGTCCACG AACATTCTTA ATACGCTGTA CTCCATCATC 
TCGCTGGGCG TATCGAATGA CCAAAATGTC GTCTCGCCAC TGGATCCGCC CGATAGCCTG 
TTCCGACTGA AGCTTGCCTG TATGCTACTG GACACGTGTG CACCCTACTT CACCAGCCAG 
GCCACGCGAA AAAAACTGGA CTACTTTCTG GTCTTCTTCC AACACTACTA TTGGTTCAAG 
AAATCCCATC CCGTGTTCAG CAAAACGGAG AACACCTCCG ATTTGTTCCC CATCCTAATG 
GATCACACCT ATCGCGACTG TCTACTCAAT GTGCGGCCCA AGCTGAAGAT TTACAAGAGT 
CTAGAGCAGG CCAAGGCGGC GATTGATCAT CTGCAGGAGA AGCTCTATCC GCAGCTAAAG 
ACTACCAATA ATGCTCAGGA TCCGTCGCTG GGCACAATCA GTGAAATAAG CGAGATAGAC 
GAGGGCGGCA CTGATGAGGA TTCTGGGTCA TCGAATGATC AGCGAGAGCG CCAAGTGTCG 
GGCCAAGAGC AGGACCAGAG CAACGACTGG ACAGAGAACG AGGCGGAGCC ACCGCTGCCG 
CCGCTCCCGC CGCCAGAAAA GTCCAAGGAG GACCTGGAGT TCGAGCAATT GTACGAAAAA 
ATGACCACAG ACTCGTACCA AGAACGGCTT AAGGAGCCGA TAAAGGCTAC GGCCAAGGAT 
ATACCTGTGC CGATGATGGC GCGCCTGCAA AAAAAATCGT ACGATCAGAT AACAGGTGCT 
CAGACCATCG GCCCTGGGAT CAACAATATG TCACAGATCT CAAAGTCGGT CCCCAATCAG 
GACGGAACGC CTGGCTCTCC AGGTGCAGAT TCACCAGATG GCAACGGCAA ATCCGGCGGC 
GGTGTAGGTG GTAGCGATGT TGGTGCTGCT GGTGCTGCTG GTGCTTCTGG TGCTACATCT 
GTACCCTTTG TGCTAATGGT GCGCGGCCAC AAGGGCGGAA AGCAGCAATT TAAGTCATTT 
GTGGCACCAT CCGACTCGCA CTTGGCCACC AACCTAAAGC GGCAGGAGCA GATGATACGC 
GAGGAGAAGG AGAAGGTCAA GCGCCTTACG CTTAACATAA CTGAGCGCAT TGAGGAGGAG 
GACTACCAGG AGTCGTTGAT GCCGCCGCAG CATCGAAACT TCACCCAGAG CTACTACCAG 
AAGCCCAATA AGCACAAGTT CAAGCACCAA AAGGGTGCGC CCGATGCGGA CCTCATATTT 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572434.1
CDS	312..4037
Translation	MLANDSATTDSEVSTPPSSRKDLADGVAEGDNDNDIDTGDTDANADEDVDAAAAIALEAEEREELQQFISELREKIESKRQLRLQNSTIELPGEEYFAKLDSNLKKNTAFVKKLKLFTATQLDGLLREISALNLSKYISEICAALVEAKLKMTDVPAVVTLASRLHCTYADFDVHFLEAWQKALNIKATEKIGNPSKLRVDLRLFAELVSSGVIQMKPGLAQLGVVLVHLIALDKDDHSNFSIILSFCRHCGEEYAGLVPQKMQQLATKYGVEVPKSDFLTADKQLNLRTMLKGYFKALCKHVLAEQAELMNMTKNIRRTMECKGEISTEKREKCELMQAGFDKLLASAQSLSELLGEPLPELAKESEGCNPGTVIDNMLDSASFGVLDPWGDEETRAFYTDLPDLRQFLPNFSAPKVDLETLEEPSELTEEAIDANLDAEMDLDDPPSTTSDTTPENPIEEQPTTPVAAAEDLKPQKMGNALMELGRQQQQQSQLNQNPIQIQNQMRQQFDGFLVNLFNCVNKELIDSAAIEFLLNFNTKHQRKKLTRTIFSVQRTRLDILPYLSRFVAIVHMCNTDVATDLAELLRKEFKWHIHKKNQLNIETKLKIVRFIGELVKFGLFKKFDALGCLKMLLRDFQHHQIEMACAFVEVTGVYLYNCRDARLLMNVFLDQMLRLKTATAMDSRHAAQIESVYYLVKPPESSKREPMVRPAMHEYIRYLIFEELCKQNVDRCIKMLRRIDWQDPETNCYAIKCLSKAYLLRFQLIRCLADLVSGLSSYQPRAVTIVIDNVFEDIRAGLEIHSPRMAQRRIAMAKYLGEMYNYKLVESTNILNTLYSIISLGVSNDQNVVSPLDPPDSLFRLKLACMLLDTCAPYFTSQATRKKLDYFLVFFQHYYWFKKSHPVFSKTENTSDLFPILMDHTYRDCLLNVRPKLKIYKSLEQAKAAIDHLQEKLYPQLKTTNNAQDPSLGTISEISEIDEGGTDEDSGSSNDQRERQVSGQEQDQSNDWTENEAEPPLPPLPPPEKSKEDLEFEQLYEKMTTDSYQERLKEPIKATAKDIPVPMMARLQKKSYDQITGAQTIGPGINNMSQISKSVPNQDGTPGSPGADSPDGNGKSGGGVGGSDVGAAGAAGASGATSVPFVLMVRGHKGGKQQFKSFVAPSDSHLATNLKRQEQMIREEKEKVKRLTLNITERIEEEDYQESLMPPQHRNFTQSYYQKPNKHKFKHQKGAPDADLIFH

Target ORF information:

RefSeq Version	NM_132206.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Upf2 (Upf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132206.3

ORF Insert Sequence:

ATGCTAGCCA ACGATTCTGC AACGACTGAC AGCGAGGTGT CCACACCGCC GTCGTCGAGG 
AAAGATTTGG CAGATGGTGT TGCCGAGGGT GACAACGACA ACGACATCGA TACCGGCGAC 
ACGGACGCAA ATGCGGATGA AGATGTGGAT GCGGCTGCTG CCATTGCCCT GGAGGCCGAA 
GAGCGCGAGG AGCTGCAACA GTTCATAAGC GAGCTGCGCG AGAAGATCGA GAGCAAGCGA 
CAGCTGCGCC TCCAAAACTC CACAATCGAG CTGCCCGGTG AGGAGTACTT CGCCAAACTG 
GACTCCAATC TCAAAAAGAA CACGGCATTC GTCAAGAAAC TGAAACTATT CACGGCCACA 
CAGCTGGACG GACTATTGCG TGAGATATCT GCTCTAAACC TGAGCAAATA CATCTCGGAA 
ATATGCGCCG CGCTGGTCGA GGCCAAACTG AAAATGACTG ATGTGCCGGC GGTGGTGACG 
CTGGCCTCTC GGCTGCACTG CACATATGCC GACTTCGATG TCCATTTTCT GGAGGCCTGG 
CAAAAGGCAC TGAACATCAA GGCCACCGAG AAGATTGGCA ATCCGAGCAA ACTGCGCGTG 
GATCTTCGCC TGTTCGCCGA ACTAGTCAGC TCTGGTGTGA TCCAAATGAA GCCCGGATTG 
GCCCAACTGG GTGTTGTTCT GGTGCATTTG ATTGCCCTGG ACAAGGATGA CCACAGCAAC 
TTCTCGATCA TCCTCTCATT CTGCCGGCAC TGCGGCGAGG AGTACGCCGG CCTAGTGCCG 
CAAAAGATGC AACAGTTGGC CACCAAATAC GGGGTGGAGG TGCCGAAATC CGACTTCCTG 
ACTGCCGACA AGCAGCTCAA TCTGCGCACC ATGCTTAAGG GTTACTTCAA GGCCCTGTGC 
AAGCATGTCC TGGCCGAGCA GGCCGAACTG ATGAACATGA CCAAGAACAT TCGGCGCACC 
ATGGAGTGCA AGGGTGAGAT ATCCACGGAG AAGCGCGAGA AGTGTGAACT GATGCAGGCC 
GGATTCGATA AGCTGCTGGC CTCCGCTCAA TCGCTGTCGG AACTGCTGGG TGAACCACTG 
CCCGAGCTGG CCAAGGAGTC TGAGGGCTGT AATCCGGGCA CAGTCATCGA CAACATGCTG 
GACAGTGCCT CCTTTGGTGT GCTGGATCCG TGGGGCGACG AGGAGACCCG AGCCTTCTAC 
ACGGATTTGC CCGATTTGAG ACAGTTTCTG CCAAACTTTT CGGCACCCAA AGTCGATTTG 
GAAACGTTAG AGGAGCCCAG TGAACTAACC GAAGAAGCGA TCGATGCCAA TCTAGATGCC 
GAAATGGATC TAGATGATCC ACCGTCCACC ACTTCGGATA CGACTCCGGA GAATCCAATC 
GAAGAGCAGC CAACGACGCC AGTTGCCGCT GCGGAGGATC TCAAGCCGCA GAAGATGGGT 
AACGCTCTAA TGGAACTGGG TCGCCAGCAG CAGCAGCAAA GTCAGCTCAA CCAGAATCCG 
ATCCAGATCC AAAACCAGAT GCGTCAGCAG TTCGATGGCT TCCTGGTCAA CCTATTCAAC 
TGTGTGAACA AGGAGCTGAT CGATTCGGCG GCCATCGAGT TCCTGCTTAA CTTCAATACC 
AAGCATCAAC GCAAGAAGCT GACGCGTACG ATATTCTCGG TGCAGCGTAC GCGTCTGGAT 
ATCCTGCCCT ATCTCTCCCG CTTCGTGGCC ATTGTGCATA TGTGCAATAC CGATGTGGCC 
ACCGATTTGG CCGAACTGCT GCGCAAGGAG TTCAAGTGGC ACATTCACAA GAAGAACCAG 
CTGAATATCG AGACGAAGCT TAAGATTGTG CGATTCATTG GTGAACTGGT TAAGTTTGGT 
TTGTTTAAAA AGTTCGATGC CTTAGGATGT CTGAAGATGC TGCTAAGGGA CTTTCAGCAT 
CATCAAATCG AGATGGCCTG TGCCTTTGTG GAAGTCACCG GCGTCTATCT GTACAATTGC 
CGGGATGCCC GTCTGCTGAT GAATGTGTTC CTGGACCAGA TGCTGCGCTT GAAAACAGCC 
ACGGCAATGG ACTCGCGACA TGCGGCGCAG ATTGAGAGCG TGTACTATTT GGTAAAGCCG 
CCGGAATCGT CCAAGCGAGA GCCCATGGTT CGGCCGGCAA TGCATGAGTA CATCCGTTAC 
TTGATCTTCG AGGAGCTTTG CAAACAGAAC GTTGATCGTT GTATCAAAAT GCTGCGCCGC 
ATCGATTGGC AGGATCCGGA GACAAATTGT TATGCCATTA AGTGCCTCAG CAAGGCGTAT 
CTGCTGCGCT TCCAGCTCAT CCGCTGTCTG GCCGATCTCG TATCCGGACT GAGCTCCTAT 
CAGCCACGAG CGGTTACCAT CGTGATCGAT AATGTGTTTG AGGATATACG CGCTGGCCTC 
GAGATTCATT CGCCACGCAT GGCACAGCGG CGCATCGCGA TGGCCAAGTA TCTGGGCGAG 
ATGTACAACT ACAAGCTGGT GGAGTCCACG AACATTCTTA ATACGCTGTA CTCCATCATC 
TCGCTGGGCG TATCGAATGA CCAAAATGTC GTCTCGCCAC TGGATCCGCC CGATAGCCTG 
TTCCGACTGA AGCTTGCCTG TATGCTACTG GACACGTGTG CACCCTACTT CACCAGCCAG 
GCCACGCGAA AAAAACTGGA CTACTTTCTG GTCTTCTTCC AACACTACTA TTGGTTCAAG 
AAATCCCATC CCGTGTTCAG CAAAACGGAG AACACCTCCG ATTTGTTCCC CATCCTAATG 
GATCACACCT ATCGCGACTG TCTACTCAAT GTGCGGCCCA AGCTGAAGAT TTACAAGAGT 
CTAGAGCAGG CCAAGGCGGC GATTGATCAT CTGCAGGAGA AGCTCTATCC GCAGCTAAAG 
ACTACCAATA ATGCTCAGGA TCCGTCGCTG GGCACAATCA GTGAAATAAG CGAGATAGAC 
GAGGGCGGCA CTGATGAGGA TTCTGGGTCA TCGAATGATC AGCGAGAGCG CCAAGTGTCG 
GGCCAAGAGC AGGACCAGAG CAACGACTGG ACAGAGAACG AGGCGGAGCC ACCGCTGCCG 
CCGCTCCCGC CGCCAGAAAA GTCCAAGGAG GACCTGGAGT TCGAGCAATT GTACGAAAAA 
ATGACCACAG ACTCGTACCA AGAACGGCTT AAGGAGCCGA TAAAGGCTAC GGCCAAGGAT 
ATACCTGTGC CGATGATGGC GCGCCTGCAA AAAAAATCGT ACGATCAGAT AACAGGTGCT 
CAGACCATCG GCCCTGGGAT CAACAATATG TCACAGATCT CAAAGTCGGT CCCCAATCAG 
GACGGAACGC CTGGCTCTCC AGGTGCAGAT TCACCAGATG GCAACGGCAA ATCCGGCGGC 
GGTGTAGGTG GTAGCGATGT TGGTGCTGCT GGTGCTGCTG GTGCTTCTGG TGCTACATCT 
GTACCCTTTG TGCTAATGGT GCGCGGCCAC AAGGGCGGAA AGCAGCAATT TAAGTCATTT 
GTGGCACCAT CCGACTCGCA CTTGGCCACC AACCTAAAGC GGCAGGAGCA GATGATACGC 
GAGGAGAAGG AGAAGGTCAA GCGCCTTACG CTTAACATAA CTGAGCGCAT TGAGGAGGAG 
GACTACCAGG AGTCGTTGAT GCCGCCGCAG CATCGAAACT TCACCCAGAG CTACTACCAG 
AAGCCCAATA AGCACAAGTT CAAGCACCAA AAGGGTGCGC CCGATGCGGA CCTCATATTT 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Upf2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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