Tbh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tbh
Entrez Gene ID	31718
Full Name	Tyramine beta hydroxylase
Synonyms	CG1543,DBH,Dbh,Dmel\CG1543,T beta h,TBH,TBetaH,TBetah,TBh,TbH,TbetaH,TbetaM,Tbetah,tbetah,tbh
General protein information	Preferred Names Tyramine beta hydroxylase Names CG1543 gene product from transcript CG1543-RB CG1543-PB CG1543-PC Tbh-PB Tbh-PC dopamine beta hydroxylase tyramine beta hydroxylase tyramine beta-hydroxylase tyramine beta-monooxygenase tyramine betahydroxylase tyramine-beta-hydroxylase tyroside beta-hydroxylase tyrosine beta hydroxylase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D2-7D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_176711.3	NP_788884.1	tyramine beta hydroxylase, isoform B
NM_001298067.1	NP_001284996.1	tyramine beta hydroxylase, isoform C

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	dbh	XP_002942324.2
Canis lupus familiaris (dog)	DBH	NP_001005263.1
Bos taurus (cattle)	DBH	NP_851338.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010485	XP_307804.4
Pan troglodytes (chimpanzee)	DBH	XP_520341.2
Danio rerio (zebrafish)	dbh	NP_001103164.1
Homo sapiens (human)	DBH	NP_000778.3
	DBH	XP_001100196.2
Mus musculus (house mouse)	Dbh	NP_620392.2
Drosophila melanogaster (fruit fly)	Tbh	NP_788884.1
Rattus norvegicus (Norway rat)	Dbh	NP_037290.2
Gallus gallus (chicken)	DBH	XP_415429.4
Caenorhabditis elegans (roundworm)	tbh-1	NP_001257264.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Interdomain long-range electron transfer becomes rate-limiting in the Y216A variant of tyramine beta-monooxygenase.
Title: Interdomain long-range electron transfer becomes rate-limiting in the Y216A variant of tyramine ?-monooxygenase.

the tyramine beta-monooxygenase mechanism is different from that of the mammalian enzyme, dopamine beta-monooxygenase
Title: Mechanism of the insect enzyme, tyramine beta-monooxygenase, reveals differences from the mammalian enzyme, dopamine beta-monooxygenase.

Octopamine is not the natural signal for flight initiation because flies lacking octopamine [tyramine-beta-hydroxylase null mutants] can fly, though they show markedly different flight-initiation and -maintenance capabilities compared to wild-type flies.
Title: Flight initiation and maintenance deficits in flies with genetically altered biogenic amine levels.

Recombinant tyramine beta-monooxygenase requires copper for activity and has a typical type 2 copper electron spin resonance (EPR) spectrum.
Title: Expression and characterization of recombinant tyramine beta-monooxygenase from Drosophila: a monomeric copper-containing hydroxylase.

Data show that ovulation is defective in females lacking tyramine beta-hydroxylase, resulting in blockage of mature oocytes within the ovaries.
Title: Distinct octopamine cell population residing in the CNS abdominal ganglion controls ovulation in Drosophila melanogaster.

The following Tbh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tbh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01065	NM_176711.3 Latest version!	Drosophila melanogaster tyramine beta hydroxylase, transcript variant B (Tbh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00
OFa01065	NM_001298067.1 Latest version!	Drosophila melanogaster tyramine beta hydroxylase, transcript variant C (Tbh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tbh ( NM_176711.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788884.1 Drosophila melanogaster tyramine beta hydroxylase, transcript variant B (Tbh), mRNA.

Price & Availability↑

CloneID	OFa01065
Clone ID Related Accession (Same CDS sequence)	NM_176711.3 , NM_001298067.1
Accession Version	NM_176711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tyramine beta hydroxylase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_176711.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTAAAA TTCCGCTGCA GCTGAGCAGT CAGGATGGCA TTTGGCCAGC CCGATTCGCC 
AGGCGACTCC ATCACCACCA CCAACTGGCT TATCATCATC ACAAGCAGGA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGCG AAACAGAAAC AAAAGCAAAA TGGAGTGCAG 
CAAGGACGTT CGCCGACATT TATGCCAGTG ATGCTGCTCC TCCTAATGGC CACACTGCTC 
ACGCGCCCGC TGAGCGCCTT CTCCAACCGC TTATCCGACA CAAAGCTGCA CGAGATCTAC 
CTGGACGACA AGGAGATTAA GCTGAGCTGG ATGGTCGACT GGTACAAGCA GGAGGTGCTC 
TTCCACTTGC AGAATGCTTT CAACGAACAG CACCGCTGGT TCTATCTGGG TTTCTCCAAG 
CGCGGCGGCC TGGCGGATGC GGATATTTGC TTTTTTGAGA ATCAGAATGG ATTCTTCAAT 
GCGGTAACCG ATACGTACAC CAGTCCGGAT GGACAGTGGG TGAGACGGGA CTACCAGCAG 
GACTGTGAGG TCTTCAAGAT GGATGAGTTC ACGTTGGCGT TTAGGCGCAA GTTTGACACC 
TGCGACCCTT TGGATTTGCG ACTCCATGAG GGCACAATGT ACGTGGTTTG GGCCCGTGGT 
GAAACGGAAC TGGCCCTGGA GGATCACCAG TTCGCTCTGC CCAATGTGAC GGCACCGCAC 
GAGGCGGGTG TTAAGATGCT ACAGCTACTA CGGGCCGACA AGATACTTAT ACCCGAAACC 
GAGTTGGATC ACATGGAGAT CACACTGCAG GAGGCGCCAA TTCCCAGTCA GGAGACCACG 
TACTGGTGTC ACGTTCAGCG ACTGGAGGGC AATCTCCGGC GTCGCCATCA TATCGTTCAG 
TTCGAGCCGC TCATCCGAAC GCCGGGCATC GTGCATCACA TGGAAGTGTT TCACTGCGAG 
GCCGGTGAGC ACGAGGAGAT TCCCCTGTAC AACGGCGACT GTGAACAGTT GCCGCCACGG 
GCCAAGATCT GCTCAAAAGT GATGGTCCTG TGGGCCATGG GCGCGGGCAC CTTTACCTAT 
CCTCCGGAAG CCGGTCTACC AATCGGCGGA CCCGGCTTCA ATCCGTACGT TCGACTGGAG 
GTACATTTCA ATAATCCGGA GAAGCAGTCG GGCTTGGTGG ACAACTCCGG CTTTCGCATC 
AAGATGTCGA AGACACTGCG TCAGTATGAC GCCGCCGTTA TGGAACTGGG TCTGGAGTAC 
ACCGACAAAA TGGCCATTCC GCCTGGCCAA ACCGCTTTCC CGCTGAGCGG CTATTGTGTG 
GCGGACTGCA CACGAGCCGC TCTGCCGGCG ACGGGCATCA TCATCTTTGG CTCTCAGCTG 
CATACGCATC TGCGTGGCGT TCGCGTCCTA ACCCGGCACT TTCGCGGCGA ACAGGAGCTG 
CGCGAGGTGA ACCGCGATGA CTACTACTCG AATCACTTCC AGGAGATGCG CACCCTGCAC 
TACAAGCCGC GTGTCCTGCC CGGCGACGCT TTGGTAACCA CTTGTTACTA CAATACCAAG 
GATGACAAGA CCGCCGCCCT CGGCGGATTC TCCATCAGCG ATGAGATGTG CGTCAACTAT 
ATCCACTACT ATCCGGCCAC CAAACTGGAG GTCTGCAAGA GTTCCGTTTC CGAGGAGACG 
CTCGAGAATT ACTTTATTTA CATGAAGCGC ACGGAGCATC AGCATGGCGT GCATTTGAAT 
GGAGCCAGGT CGTCCAATTA CCGGAGCATC GAATGGACCC AGCCGCGTAT CGATCAGCTG 
TACACCATGT ACATGCAGGA GCCGCTGAGC ATGCAGTGCA ACAGGTCCGA TGGCACTCGC 
TTCGAGGGGC GGTCTAGCTG GGAGGGCGTG GCTGCGACGC CCGTACAAAT TCGCATACCC 
ATTCACCGCA AACTGTGCCC CAACTACAAT CCGCTGTGGC TGAAGCCATT GGAGAAGGGC 
GATTGCGATT TGCTGGGGGA GTGCATCTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788884.1
CDS	215..2227
Translation	MLKIPLQLSSQDGIWPARFARRLHHHHQLAYHHHKQEQQQQQQQQQQQQAKQKQKQNGVQQGRSPTFMPVMLLLLMATLLTRPLSAFSNRLSDTKLHEIYLDDKEIKLSWMVDWYKQEVLFHLQNAFNEQHRWFYLGFSKRGGLADADICFFENQNGFFNAVTDTYTSPDGQWVRRDYQQDCEVFKMDEFTLAFRRKFDTCDPLDLRLHEGTMYVVWARGETELALEDHQFALPNVTAPHEAGVKMLQLLRADKILIPETELDHMEITLQEAPIPSQETTYWCHVQRLEGNLRRRHHIVQFEPLIRTPGIVHHMEVFHCEAGEHEEIPLYNGDCEQLPPRAKICSKVMVLWAMGAGTFTYPPEAGLPIGGPGFNPYVRLEVHFNNPEKQSGLVDNSGFRIKMSKTLRQYDAAVMELGLEYTDKMAIPPGQTAFPLSGYCVADCTRAALPATGIIIFGSQLHTHLRGVRVLTRHFRGEQELREVNRDDYYSNHFQEMRTLHYKPRVLPGDALVTTCYYNTKDDKTAALGGFSISDEMCVNYIHYYPATKLEVCKSSVSEETLENYFIYMKRTEHQHGVHLNGARSSNYRSIEWTQPRIDQLYTMYMQEPLSMQCNRSDGTRFEGRSSWEGVAATPVQIRIPIHRKLCPNYNPLWLKPLEKGDCDLLGECIY

Target ORF information:

RefSeq Version	NM_176711.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tyramine beta hydroxylase, transcript variant B (Tbh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176711.3

ORF Insert Sequence:

ATGCTTAAAA TTCCGCTGCA GCTGAGCAGT CAGGATGGCA TTTGGCCAGC CCGATTCGCC 
AGGCGACTCC ATCACCACCA CCAACTGGCT TATCATCATC ACAAGCAGGA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGCG AAACAGAAAC AAAAGCAAAA TGGAGTGCAG 
CAAGGACGTT CGCCGACATT TATGCCAGTG ATGCTGCTCC TCCTAATGGC CACACTGCTC 
ACGCGCCCGC TGAGCGCCTT CTCCAACCGC TTATCCGACA CAAAGCTGCA CGAGATCTAC 
CTGGACGACA AGGAGATTAA GCTGAGCTGG ATGGTCGACT GGTACAAGCA GGAGGTGCTC 
TTCCACTTGC AGAATGCTTT CAACGAACAG CACCGCTGGT TCTATCTGGG TTTCTCCAAG 
CGCGGCGGCC TGGCGGATGC GGATATTTGC TTTTTTGAGA ATCAGAATGG ATTCTTCAAT 
GCGGTAACCG ATACGTACAC CAGTCCGGAT GGACAGTGGG TGAGACGGGA CTACCAGCAG 
GACTGTGAGG TCTTCAAGAT GGATGAGTTC ACGTTGGCGT TTAGGCGCAA GTTTGACACC 
TGCGACCCTT TGGATTTGCG ACTCCATGAG GGCACAATGT ACGTGGTTTG GGCCCGTGGT 
GAAACGGAAC TGGCCCTGGA GGATCACCAG TTCGCTCTGC CCAATGTGAC GGCACCGCAC 
GAGGCGGGTG TTAAGATGCT ACAGCTACTA CGGGCCGACA AGATACTTAT ACCCGAAACC 
GAGTTGGATC ACATGGAGAT CACACTGCAG GAGGCGCCAA TTCCCAGTCA GGAGACCACG 
TACTGGTGTC ACGTTCAGCG ACTGGAGGGC AATCTCCGGC GTCGCCATCA TATCGTTCAG 
TTCGAGCCGC TCATCCGAAC GCCGGGCATC GTGCATCACA TGGAAGTGTT TCACTGCGAG 
GCCGGTGAGC ACGAGGAGAT TCCCCTGTAC AACGGCGACT GTGAACAGTT GCCGCCACGG 
GCCAAGATCT GCTCAAAAGT GATGGTCCTG TGGGCCATGG GCGCGGGCAC CTTTACCTAT 
CCTCCGGAAG CCGGTCTACC AATCGGCGGA CCCGGCTTCA ATCCGTACGT TCGACTGGAG 
GTACATTTCA ATAATCCGGA GAAGCAGTCG GGCTTGGTGG ACAACTCCGG CTTTCGCATC 
AAGATGTCGA AGACACTGCG TCAGTATGAC GCCGCCGTTA TGGAACTGGG TCTGGAGTAC 
ACCGACAAAA TGGCCATTCC GCCTGGCCAA ACCGCTTTCC CGCTGAGCGG CTATTGTGTG 
GCGGACTGCA CACGAGCCGC TCTGCCGGCG ACGGGCATCA TCATCTTTGG CTCTCAGCTG 
CATACGCATC TGCGTGGCGT TCGCGTCCTA ACCCGGCACT TTCGCGGCGA ACAGGAGCTG 
CGCGAGGTGA ACCGCGATGA CTACTACTCG AATCACTTCC AGGAGATGCG CACCCTGCAC 
TACAAGCCGC GTGTCCTGCC CGGCGACGCT TTGGTAACCA CTTGTTACTA CAATACCAAG 
GATGACAAGA CCGCCGCCCT CGGCGGATTC TCCATCAGCG ATGAGATGTG CGTCAACTAT 
ATCCACTACT ATCCGGCCAC CAAACTGGAG GTCTGCAAGA GTTCCGTTTC CGAGGAGACG 
CTCGAGAATT ACTTTATTTA CATGAAGCGC ACGGAGCATC AGCATGGCGT GCATTTGAAT 
GGAGCCAGGT CGTCCAATTA CCGGAGCATC GAATGGACCC AGCCGCGTAT CGATCAGCTG 
TACACCATGT ACATGCAGGA GCCGCTGAGC ATGCAGTGCA ACAGGTCCGA TGGCACTCGC 
TTCGAGGGGC GGTCTAGCTG GGAGGGCGTG GCTGCGACGC CCGTACAAAT TCGCATACCC 
ATTCACCGCA AACTGTGCCC CAACTACAAT CCGCTGTGGC TGAAGCCATT GGAGAAGGGC 
GATTGCGATT TGCTGGGGGA GTGCATCTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tbh ( NM_001298067.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284996.1 Drosophila melanogaster tyramine beta hydroxylase, transcript variant C (Tbh), mRNA.

Price & Availability↑

CloneID	OFa01065
Clone ID Related Accession (Same CDS sequence)	NM_176711.3 , NM_001298067.1
Accession Version	NM_001298067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tyramine beta hydroxylase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTAAAA TTCCGCTGCA GCTGAGCAGT CAGGATGGCA TTTGGCCAGC CCGATTCGCC 
AGGCGACTCC ATCACCACCA CCAACTGGCT TATCATCATC ACAAGCAGGA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGCG AAACAGAAAC AAAAGCAAAA TGGAGTGCAG 
CAAGGACGTT CGCCGACATT TATGCCAGTG ATGCTGCTCC TCCTAATGGC CACACTGCTC 
ACGCGCCCGC TGAGCGCCTT CTCCAACCGC TTATCCGACA CAAAGCTGCA CGAGATCTAC 
CTGGACGACA AGGAGATTAA GCTGAGCTGG ATGGTCGACT GGTACAAGCA GGAGGTGCTC 
TTCCACTTGC AGAATGCTTT CAACGAACAG CACCGCTGGT TCTATCTGGG TTTCTCCAAG 
CGCGGCGGCC TGGCGGATGC GGATATTTGC TTTTTTGAGA ATCAGAATGG ATTCTTCAAT 
GCGGTAACCG ATACGTACAC CAGTCCGGAT GGACAGTGGG TGAGACGGGA CTACCAGCAG 
GACTGTGAGG TCTTCAAGAT GGATGAGTTC ACGTTGGCGT TTAGGCGCAA GTTTGACACC 
TGCGACCCTT TGGATTTGCG ACTCCATGAG GGCACAATGT ACGTGGTTTG GGCCCGTGGT 
GAAACGGAAC TGGCCCTGGA GGATCACCAG TTCGCTCTGC CCAATGTGAC GGCACCGCAC 
GAGGCGGGTG TTAAGATGCT ACAGCTACTA CGGGCCGACA AGATACTTAT ACCCGAAACC 
GAGTTGGATC ACATGGAGAT CACACTGCAG GAGGCGCCAA TTCCCAGTCA GGAGACCACG 
TACTGGTGTC ACGTTCAGCG ACTGGAGGGC AATCTCCGGC GTCGCCATCA TATCGTTCAG 
TTCGAGCCGC TCATCCGAAC GCCGGGCATC GTGCATCACA TGGAAGTGTT TCACTGCGAG 
GCCGGTGAGC ACGAGGAGAT TCCCCTGTAC AACGGCGACT GTGAACAGTT GCCGCCACGG 
GCCAAGATCT GCTCAAAAGT GATGGTCCTG TGGGCCATGG GCGCGGGCAC CTTTACCTAT 
CCTCCGGAAG CCGGTCTACC AATCGGCGGA CCCGGCTTCA ATCCGTACGT TCGACTGGAG 
GTACATTTCA ATAATCCGGA GAAGCAGTCG GGCTTGGTGG ACAACTCCGG CTTTCGCATC 
AAGATGTCGA AGACACTGCG TCAGTATGAC GCCGCCGTTA TGGAACTGGG TCTGGAGTAC 
ACCGACAAAA TGGCCATTCC GCCTGGCCAA ACCGCTTTCC CGCTGAGCGG CTATTGTGTG 
GCGGACTGCA CACGAGCCGC TCTGCCGGCG ACGGGCATCA TCATCTTTGG CTCTCAGCTG 
CATACGCATC TGCGTGGCGT TCGCGTCCTA ACCCGGCACT TTCGCGGCGA ACAGGAGCTG 
CGCGAGGTGA ACCGCGATGA CTACTACTCG AATCACTTCC AGGAGATGCG CACCCTGCAC 
TACAAGCCGC GTGTCCTGCC CGGCGACGCT TTGGTAACCA CTTGTTACTA CAATACCAAG 
GATGACAAGA CCGCCGCCCT CGGCGGATTC TCCATCAGCG ATGAGATGTG CGTCAACTAT 
ATCCACTACT ATCCGGCCAC CAAACTGGAG GTCTGCAAGA GTTCCGTTTC CGAGGAGACG 
CTCGAGAATT ACTTTATTTA CATGAAGCGC ACGGAGCATC AGCATGGCGT GCATTTGAAT 
GGAGCCAGGT CGTCCAATTA CCGGAGCATC GAATGGACCC AGCCGCGTAT CGATCAGCTG 
TACACCATGT ACATGCAGGA GCCGCTGAGC ATGCAGTGCA ACAGGTCCGA TGGCACTCGC 
TTCGAGGGGC GGTCTAGCTG GGAGGGCGTG GCTGCGACGC CCGTACAAAT TCGCATACCC 
ATTCACCGCA AACTGTGCCC CAACTACAAT CCGCTGTGGC TGAAGCCATT GGAGAAGGGC 
GATTGCGATT TGCTGGGGGA GTGCATCTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284996.1
CDS	214..2226
Translation	MLKIPLQLSSQDGIWPARFARRLHHHHQLAYHHHKQEQQQQQQQQQQQQAKQKQKQNGVQQGRSPTFMPVMLLLLMATLLTRPLSAFSNRLSDTKLHEIYLDDKEIKLSWMVDWYKQEVLFHLQNAFNEQHRWFYLGFSKRGGLADADICFFENQNGFFNAVTDTYTSPDGQWVRRDYQQDCEVFKMDEFTLAFRRKFDTCDPLDLRLHEGTMYVVWARGETELALEDHQFALPNVTAPHEAGVKMLQLLRADKILIPETELDHMEITLQEAPIPSQETTYWCHVQRLEGNLRRRHHIVQFEPLIRTPGIVHHMEVFHCEAGEHEEIPLYNGDCEQLPPRAKICSKVMVLWAMGAGTFTYPPEAGLPIGGPGFNPYVRLEVHFNNPEKQSGLVDNSGFRIKMSKTLRQYDAAVMELGLEYTDKMAIPPGQTAFPLSGYCVADCTRAALPATGIIIFGSQLHTHLRGVRVLTRHFRGEQELREVNRDDYYSNHFQEMRTLHYKPRVLPGDALVTTCYYNTKDDKTAALGGFSISDEMCVNYIHYYPATKLEVCKSSVSEETLENYFIYMKRTEHQHGVHLNGARSSNYRSIEWTQPRIDQLYTMYMQEPLSMQCNRSDGTRFEGRSSWEGVAATPVQIRIPIHRKLCPNYNPLWLKPLEKGDCDLLGECIY

Target ORF information:

RefSeq Version	NM_001298067.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tyramine beta hydroxylase, transcript variant C (Tbh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298067.1

ORF Insert Sequence:

ATGCTTAAAA TTCCGCTGCA GCTGAGCAGT CAGGATGGCA TTTGGCCAGC CCGATTCGCC 
AGGCGACTCC ATCACCACCA CCAACTGGCT TATCATCATC ACAAGCAGGA GCAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGCG AAACAGAAAC AAAAGCAAAA TGGAGTGCAG 
CAAGGACGTT CGCCGACATT TATGCCAGTG ATGCTGCTCC TCCTAATGGC CACACTGCTC 
ACGCGCCCGC TGAGCGCCTT CTCCAACCGC TTATCCGACA CAAAGCTGCA CGAGATCTAC 
CTGGACGACA AGGAGATTAA GCTGAGCTGG ATGGTCGACT GGTACAAGCA GGAGGTGCTC 
TTCCACTTGC AGAATGCTTT CAACGAACAG CACCGCTGGT TCTATCTGGG TTTCTCCAAG 
CGCGGCGGCC TGGCGGATGC GGATATTTGC TTTTTTGAGA ATCAGAATGG ATTCTTCAAT 
GCGGTAACCG ATACGTACAC CAGTCCGGAT GGACAGTGGG TGAGACGGGA CTACCAGCAG 
GACTGTGAGG TCTTCAAGAT GGATGAGTTC ACGTTGGCGT TTAGGCGCAA GTTTGACACC 
TGCGACCCTT TGGATTTGCG ACTCCATGAG GGCACAATGT ACGTGGTTTG GGCCCGTGGT 
GAAACGGAAC TGGCCCTGGA GGATCACCAG TTCGCTCTGC CCAATGTGAC GGCACCGCAC 
GAGGCGGGTG TTAAGATGCT ACAGCTACTA CGGGCCGACA AGATACTTAT ACCCGAAACC 
GAGTTGGATC ACATGGAGAT CACACTGCAG GAGGCGCCAA TTCCCAGTCA GGAGACCACG 
TACTGGTGTC ACGTTCAGCG ACTGGAGGGC AATCTCCGGC GTCGCCATCA TATCGTTCAG 
TTCGAGCCGC TCATCCGAAC GCCGGGCATC GTGCATCACA TGGAAGTGTT TCACTGCGAG 
GCCGGTGAGC ACGAGGAGAT TCCCCTGTAC AACGGCGACT GTGAACAGTT GCCGCCACGG 
GCCAAGATCT GCTCAAAAGT GATGGTCCTG TGGGCCATGG GCGCGGGCAC CTTTACCTAT 
CCTCCGGAAG CCGGTCTACC AATCGGCGGA CCCGGCTTCA ATCCGTACGT TCGACTGGAG 
GTACATTTCA ATAATCCGGA GAAGCAGTCG GGCTTGGTGG ACAACTCCGG CTTTCGCATC 
AAGATGTCGA AGACACTGCG TCAGTATGAC GCCGCCGTTA TGGAACTGGG TCTGGAGTAC 
ACCGACAAAA TGGCCATTCC GCCTGGCCAA ACCGCTTTCC CGCTGAGCGG CTATTGTGTG 
GCGGACTGCA CACGAGCCGC TCTGCCGGCG ACGGGCATCA TCATCTTTGG CTCTCAGCTG 
CATACGCATC TGCGTGGCGT TCGCGTCCTA ACCCGGCACT TTCGCGGCGA ACAGGAGCTG 
CGCGAGGTGA ACCGCGATGA CTACTACTCG AATCACTTCC AGGAGATGCG CACCCTGCAC 
TACAAGCCGC GTGTCCTGCC CGGCGACGCT TTGGTAACCA CTTGTTACTA CAATACCAAG 
GATGACAAGA CCGCCGCCCT CGGCGGATTC TCCATCAGCG ATGAGATGTG CGTCAACTAT 
ATCCACTACT ATCCGGCCAC CAAACTGGAG GTCTGCAAGA GTTCCGTTTC CGAGGAGACG 
CTCGAGAATT ACTTTATTTA CATGAAGCGC ACGGAGCATC AGCATGGCGT GCATTTGAAT 
GGAGCCAGGT CGTCCAATTA CCGGAGCATC GAATGGACCC AGCCGCGTAT CGATCAGCTG 
TACACCATGT ACATGCAGGA GCCGCTGAGC ATGCAGTGCA ACAGGTCCGA TGGCACTCGC 
TTCGAGGGGC GGTCTAGCTG GGAGGGCGTG GCTGCGACGC CCGTACAAAT TCGCATACCC 
ATTCACCGCA AACTGTGCCC CAACTACAAT CCGCTGTGGC TGAAGCCATT GGAGAAGGGC 
GATTGCGATT TGCTGGGGGA GTGCATCTAT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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Tbh cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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