p115 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	p115
Entrez Gene ID	31698
Full Name	CG1422 gene product from transcript CG1422-RB
Synonyms	CG1422,Dmel\CG1422,dp115,l(1)G0306
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7C2-7C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_132189.3	NP_572417.1	p115, isoform A
NM_001298063.1	NP_001284992.1	p115, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Uso1	NP_062363.1
Danio rerio (zebrafish)	uso1	NP_956449.1
Drosophila melanogaster (fruit fly)	p115	NP_572417.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009840	XP_318953.4
Neurospora crassa	NCU01644	XP_956266.1
Canis lupus familiaris (dog)	USO1	XP_005639121.1
Bos taurus (cattle)	USO1	NP_777270.2
Gallus gallus (chicken)	USO1	NP_001026310.1
Caenorhabditis elegans (roundworm)	uso-1	NP_001040974.1
Kluyveromyces lactis	KLLA0E05281g	XP_454183.1
Homo sapiens (human)	USO1	NP_003706.1
Saccharomyces cerevisiae (baker's yeast)	USO1	NP_010225.1
	USO1	XP_001100314.2
Rattus norvegicus (Norway rat)	Uso1	NP_062252.1
Danio rerio (zebrafish)	LOC100334773	XP_002660510.1
Magnaporthe oryzae (rice blast fungus)	MGG_17317	XP_003717804.1
Pan troglodytes (chimpanzee)	USO1	XP_001153352.1
Eremothecium gossypii	AGOS_AER045C	NP_984905.1
Xenopus tropicalis (tropical clawed frog)	uso1	XP_004911135.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Depletion of p115 by RNAi in Drosophila S2 cells led to important morphological changes in the Golgi apparatus and the transitional ER.
Title: A novel role for dp115 in the organization of tER sites in Drosophila.

The following p115 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the p115 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01045	NM_001298063.1 Latest version!	Drosophila melanogaster p115, transcript variant B (p115), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa01045	NM_132189.3 Latest version!	Drosophila melanogaster p115, transcript variant A (p115), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

p115 ( NM_132189.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572417.1 Drosophila melanogaster p115, transcript variant A (p115), mRNA.

Price & Availability↑

CloneID	OFa01045
Clone ID Related Accession (Same CDS sequence)	NM_132189.3 , NM_001298063.1
Accession Version	NM_132189.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	p115, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132189.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTTCC TGAAGAGTGG CATCAAAACG GTGCTGGGCA GCACGGAGCC GGGTCAGCAG 
CCGAGTGCCG CCGAGACGGT GGAGAAGCTG GTGGACCGTG TCTACTCCTC AACGCTCCTG 
GAGGATCGAC GGGATGCCTG CCGAGCGCTA AAAGCCCTGT CCCGCAAGTA CCGCATCGAG 
GTGGGCGCAC AGGGCATGCC TCCGCTGGTC CAGGTGCTCC AGAACGACGG ACAGGATGCA 
GAGATCATCA GCTATGCGCT GGACACACTG TGCAACGTGG TTACTAGCGA GGAGTTCGAC 
GAGGAGGCTG ATAATCCCAC CGTTTCGGTG AATGTGGGCG AGCAGTTCAC GGAGATGTTC 
ATCAAGACAC CGGAGCATGT AACCCTGGTA ATGGGCTATC TGGACGAATA CGATTTTCGA 
GTGCGTCGCG CTGCCATCCA ACTGATTACG TCCCTCATTT CGAACAAGAC GCGGGAGCTG 
CAGGATTTGA TTCTGGTCAG TCCGATGGGC GTTTCCAAGC TGATGGATCT GCTGACGGAC 
AGCCGCGAAG TGATTCGCAA CGATGTCCTT CTGCTGCTTA TCGAACTGAC CAAGGGCAAC 
TCAAACATCC AAAAGATTGT GGCCTTTGAG AATGCCTTTG ACCGCCTTTT TGAAATTGTC 
CGTGAGGAGG GCTGTTCGGA TGGCGGAATT GTTGTGGAGG ATTGCCTAAT TCTGCTATTG 
AATCTTCTCA AGAACAACTC CAGTAATCAG CAGTTCTTTA AGGAGGGCAG CTATATCCAG 
CGATTGTCGC CAATGTTTGA GCTCTCACAG GATGCGGAAG AGGTTGGCTG GTCGCCACAG 
AAGGTCTCAA ACTTCCATTG TCTGCTGCAG GTGGTGCGGG CACTGGTCAC GCCCAGTAAT 
CAACAGCAGG TGGTGGCCGC CTGCCAGCGG GTCATGCAAA AGTCTCGACT GCTCCATGCT 
CTTTGCGAGA TTCTGATGAG CAGTGGTGTC CCTGCGGATA TACTCACGGA AACCATCAAT 
GCAGTGGCCG AAGTGGTGCG CGGCGATCGG GACAACCAGG ATGAATTGGG AAGAGTTATG 
GCTCCGAGCA GTCCACCCAG ACCTGCCATC GTGGTCCTTC TTATGTCCAT GATCAATGAG 
AAACAACTGC TGGCACTGCG TTGCGCCGTA CTCTACTGTT TCGAGTGCTT TCTTTATCGC 
AATGCCGATG GCCAGCGGGC GGTTGTACAG ACCCTGCTGC CCTCCAGTGC GTCCGATGTG 
AGTGCCCTTT CGACGGGACA ACTCCTTTGC ACCGGACTCT TCTCCACAGA TGCGCTTGCC 
AACTGGTTCT CCGCTGTGGC CCTAATGCAC TCACTCGTAG AGAATGTCGC CCTAAAGGAG 
GAACTACTGC GCGTACTGCT GGCCACTCCT GGTGGCCAAA AGCCTATCAC GCTACTCGAG 
CAGTGCACTA ATCTCATGCA GCAGGAACGC TACCGTTTGC AAAGCAAGGT GGGTCTGCTA 
ATGCTACTCA GCCTGTGGCT GGCGCATTGT CCAGGTGCGG TGAAGGCTTT GCTGGAAACG 
CAGGGCACCA TGGCCTACCT GACCGCTCAA CTTTGCTCCA ACGAGCACGA TGAACGAGAG 
TTCCTTGTCC AGGGCATGTG CGCATTCCTA ATGGGCCTGT GCATCCAGTT CAATGACAAC 
TCGCTGCCGG GGCAGAAGCG CGAGGACATC AGTCAGCTGA TCATCAAGCG AATTGGCCAG 
GAGAGCTTCT GTAGCAAACT AGCAGAAGTG TCGCGACATG AGGCCTACAG TCGGGCATGC 
AAGCAGGCCC AGATCCGTGC CAAATCCGCT GGGGAACTAC TGCTTGACTT CGAGTATTGC 
AAGCTGTACA AGGGACTGGA GGCGTTGATT GCCAAGCTGG TTAGCGGATT TGATGTAGAT 
GGCATTGAAC TGACCGAACT CACGTTGAGC TCGGAAGCTT CTGCATTGGT TTCCCAGTAC 
AAGGGGATTA TCCGCGGCAT GGATGCCCAG ATCCAAGCAC TGCAGCAATC CTCCAAAGAA 
CTGGAACAGG AGAATGCCGA GCTGAAGGAG AAACTTGGTG AGGAGCAGTC GCTCAAGGCG 
CAGCTCTTGG ACCAGAACAC GCTGCTTAAG GCACAGCTGG GAGCCTCGAC CGGTCAGGTG 
CAGTCCGCCC AAGGTGCGGA AGCCACGCCG CCCAACGAGG AGGAGCTAAA TGCCGCTCGC 
TACCAGGCCA ACATGTACTT TGCCGAAAAC ATTCGTCTAA CTAAGGAGCT GGAGACACTA 
CGTCAGCAGT TATCAGCCGA AAAACAGAGT GCAGATGCGG CGCAAGATTC CCTGGCGGCG 
ATGCAAAAGG ATCAAGAAGA TTTGCTCGAA CTGCTGGCCG ATCAGGAGGC AAAGCTAACG 
CGATACGAAG AAGCGGGCTC GACTAATACC CTTCCGACTT CCAATGTAGC TCCAAGCGTG 
CCGGCAGCTG GCGGAGGAAG CCCCATCCCA AGTGGCACCG CCAGCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572417.1
CDS	97..2607
Translation	MEFLKSGIKTVLGSTEPGQQPSAAETVEKLVDRVYSSTLLEDRRDACRALKALSRKYRIEVGAQGMPPLVQVLQNDGQDAEIISYALDTLCNVVTSEEFDEEADNPTVSVNVGEQFTEMFIKTPEHVTLVMGYLDEYDFRVRRAAIQLITSLISNKTRELQDLILVSPMGVSKLMDLLTDSREVIRNDVLLLLIELTKGNSNIQKIVAFENAFDRLFEIVREEGCSDGGIVVEDCLILLLNLLKNNSSNQQFFKEGSYIQRLSPMFELSQDAEEVGWSPQKVSNFHCLLQVVRALVTPSNQQQVVAACQRVMQKSRLLHALCEILMSSGVPADILTETINAVAEVVRGDRDNQDELGRVMAPSSPPRPAIVVLLMSMINEKQLLALRCAVLYCFECFLYRNADGQRAVVQTLLPSSASDVSALSTGQLLCTGLFSTDALANWFSAVALMHSLVENVALKEELLRVLLATPGGQKPITLLEQCTNLMQQERYRLQSKVGLLMLLSLWLAHCPGAVKALLETQGTMAYLTAQLCSNEHDEREFLVQGMCAFLMGLCIQFNDNSLPGQKREDISQLIIKRIGQESFCSKLAEVSRHEAYSRACKQAQIRAKSAGELLLDFEYCKLYKGLEALIAKLVSGFDVDGIELTELTLSSEASALVSQYKGIIRGMDAQIQALQQSSKELEQENAELKEKLGEEQSLKAQLLDQNTLLKAQLGASTGQVQSAQGAEATPPNEEELNAARYQANMYFAENIRLTKELETLRQQLSAEKQSADAAQDSLAAMQKDQEDLLELLADQEAKLTRYEEAGSTNTLPTSNVAPSVPAAGGGSPIPSGTASR

Target ORF information:

RefSeq Version	NM_132189.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster p115, transcript variant A (p115), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132189.3

ORF Insert Sequence:

ATGGAGTTCC TGAAGAGTGG CATCAAAACG GTGCTGGGCA GCACGGAGCC GGGTCAGCAG 
CCGAGTGCCG CCGAGACGGT GGAGAAGCTG GTGGACCGTG TCTACTCCTC AACGCTCCTG 
GAGGATCGAC GGGATGCCTG CCGAGCGCTA AAAGCCCTGT CCCGCAAGTA CCGCATCGAG 
GTGGGCGCAC AGGGCATGCC TCCGCTGGTC CAGGTGCTCC AGAACGACGG ACAGGATGCA 
GAGATCATCA GCTATGCGCT GGACACACTG TGCAACGTGG TTACTAGCGA GGAGTTCGAC 
GAGGAGGCTG ATAATCCCAC CGTTTCGGTG AATGTGGGCG AGCAGTTCAC GGAGATGTTC 
ATCAAGACAC CGGAGCATGT AACCCTGGTA ATGGGCTATC TGGACGAATA CGATTTTCGA 
GTGCGTCGCG CTGCCATCCA ACTGATTACG TCCCTCATTT CGAACAAGAC GCGGGAGCTG 
CAGGATTTGA TTCTGGTCAG TCCGATGGGC GTTTCCAAGC TGATGGATCT GCTGACGGAC 
AGCCGCGAAG TGATTCGCAA CGATGTCCTT CTGCTGCTTA TCGAACTGAC CAAGGGCAAC 
TCAAACATCC AAAAGATTGT GGCCTTTGAG AATGCCTTTG ACCGCCTTTT TGAAATTGTC 
CGTGAGGAGG GCTGTTCGGA TGGCGGAATT GTTGTGGAGG ATTGCCTAAT TCTGCTATTG 
AATCTTCTCA AGAACAACTC CAGTAATCAG CAGTTCTTTA AGGAGGGCAG CTATATCCAG 
CGATTGTCGC CAATGTTTGA GCTCTCACAG GATGCGGAAG AGGTTGGCTG GTCGCCACAG 
AAGGTCTCAA ACTTCCATTG TCTGCTGCAG GTGGTGCGGG CACTGGTCAC GCCCAGTAAT 
CAACAGCAGG TGGTGGCCGC CTGCCAGCGG GTCATGCAAA AGTCTCGACT GCTCCATGCT 
CTTTGCGAGA TTCTGATGAG CAGTGGTGTC CCTGCGGATA TACTCACGGA AACCATCAAT 
GCAGTGGCCG AAGTGGTGCG CGGCGATCGG GACAACCAGG ATGAATTGGG AAGAGTTATG 
GCTCCGAGCA GTCCACCCAG ACCTGCCATC GTGGTCCTTC TTATGTCCAT GATCAATGAG 
AAACAACTGC TGGCACTGCG TTGCGCCGTA CTCTACTGTT TCGAGTGCTT TCTTTATCGC 
AATGCCGATG GCCAGCGGGC GGTTGTACAG ACCCTGCTGC CCTCCAGTGC GTCCGATGTG 
AGTGCCCTTT CGACGGGACA ACTCCTTTGC ACCGGACTCT TCTCCACAGA TGCGCTTGCC 
AACTGGTTCT CCGCTGTGGC CCTAATGCAC TCACTCGTAG AGAATGTCGC CCTAAAGGAG 
GAACTACTGC GCGTACTGCT GGCCACTCCT GGTGGCCAAA AGCCTATCAC GCTACTCGAG 
CAGTGCACTA ATCTCATGCA GCAGGAACGC TACCGTTTGC AAAGCAAGGT GGGTCTGCTA 
ATGCTACTCA GCCTGTGGCT GGCGCATTGT CCAGGTGCGG TGAAGGCTTT GCTGGAAACG 
CAGGGCACCA TGGCCTACCT GACCGCTCAA CTTTGCTCCA ACGAGCACGA TGAACGAGAG 
TTCCTTGTCC AGGGCATGTG CGCATTCCTA ATGGGCCTGT GCATCCAGTT CAATGACAAC 
TCGCTGCCGG GGCAGAAGCG CGAGGACATC AGTCAGCTGA TCATCAAGCG AATTGGCCAG 
GAGAGCTTCT GTAGCAAACT AGCAGAAGTG TCGCGACATG AGGCCTACAG TCGGGCATGC 
AAGCAGGCCC AGATCCGTGC CAAATCCGCT GGGGAACTAC TGCTTGACTT CGAGTATTGC 
AAGCTGTACA AGGGACTGGA GGCGTTGATT GCCAAGCTGG TTAGCGGATT TGATGTAGAT 
GGCATTGAAC TGACCGAACT CACGTTGAGC TCGGAAGCTT CTGCATTGGT TTCCCAGTAC 
AAGGGGATTA TCCGCGGCAT GGATGCCCAG ATCCAAGCAC TGCAGCAATC CTCCAAAGAA 
CTGGAACAGG AGAATGCCGA GCTGAAGGAG AAACTTGGTG AGGAGCAGTC GCTCAAGGCG 
CAGCTCTTGG ACCAGAACAC GCTGCTTAAG GCACAGCTGG GAGCCTCGAC CGGTCAGGTG 
CAGTCCGCCC AAGGTGCGGA AGCCACGCCG CCCAACGAGG AGGAGCTAAA TGCCGCTCGC 
TACCAGGCCA ACATGTACTT TGCCGAAAAC ATTCGTCTAA CTAAGGAGCT GGAGACACTA 
CGTCAGCAGT TATCAGCCGA AAAACAGAGT GCAGATGCGG CGCAAGATTC CCTGGCGGCG 
ATGCAAAAGG ATCAAGAAGA TTTGCTCGAA CTGCTGGCCG ATCAGGAGGC AAAGCTAACG 
CGATACGAAG AAGCGGGCTC GACTAATACC CTTCCGACTT CCAATGTAGC TCCAAGCGTG 
CCGGCAGCTG GCGGAGGAAG CCCCATCCCA AGTGGCACCG CCAGCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

p115 ( NM_001298063.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284992.1 Drosophila melanogaster p115, transcript variant B (p115), mRNA.

Price & Availability↑

CloneID	OFa01045
Clone ID Related Accession (Same CDS sequence)	NM_132189.3 , NM_001298063.1
Accession Version	NM_001298063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	p115, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTTCC TGAAGAGTGG CATCAAAACG GTGCTGGGCA GCACGGAGCC GGGTCAGCAG 
CCGAGTGCCG CCGAGACGGT GGAGAAGCTG GTGGACCGTG TCTACTCCTC AACGCTCCTG 
GAGGATCGAC GGGATGCCTG CCGAGCGCTA AAAGCCCTGT CCCGCAAGTA CCGCATCGAG 
GTGGGCGCAC AGGGCATGCC TCCGCTGGTC CAGGTGCTCC AGAACGACGG ACAGGATGCA 
GAGATCATCA GCTATGCGCT GGACACACTG TGCAACGTGG TTACTAGCGA GGAGTTCGAC 
GAGGAGGCTG ATAATCCCAC CGTTTCGGTG AATGTGGGCG AGCAGTTCAC GGAGATGTTC 
ATCAAGACAC CGGAGCATGT AACCCTGGTA ATGGGCTATC TGGACGAATA CGATTTTCGA 
GTGCGTCGCG CTGCCATCCA ACTGATTACG TCCCTCATTT CGAACAAGAC GCGGGAGCTG 
CAGGATTTGA TTCTGGTCAG TCCGATGGGC GTTTCCAAGC TGATGGATCT GCTGACGGAC 
AGCCGCGAAG TGATTCGCAA CGATGTCCTT CTGCTGCTTA TCGAACTGAC CAAGGGCAAC 
TCAAACATCC AAAAGATTGT GGCCTTTGAG AATGCCTTTG ACCGCCTTTT TGAAATTGTC 
CGTGAGGAGG GCTGTTCGGA TGGCGGAATT GTTGTGGAGG ATTGCCTAAT TCTGCTATTG 
AATCTTCTCA AGAACAACTC CAGTAATCAG CAGTTCTTTA AGGAGGGCAG CTATATCCAG 
CGATTGTCGC CAATGTTTGA GCTCTCACAG GATGCGGAAG AGGTTGGCTG GTCGCCACAG 
AAGGTCTCAA ACTTCCATTG TCTGCTGCAG GTGGTGCGGG CACTGGTCAC GCCCAGTAAT 
CAACAGCAGG TGGTGGCCGC CTGCCAGCGG GTCATGCAAA AGTCTCGACT GCTCCATGCT 
CTTTGCGAGA TTCTGATGAG CAGTGGTGTC CCTGCGGATA TACTCACGGA AACCATCAAT 
GCAGTGGCCG AAGTGGTGCG CGGCGATCGG GACAACCAGG ATGAATTGGG AAGAGTTATG 
GCTCCGAGCA GTCCACCCAG ACCTGCCATC GTGGTCCTTC TTATGTCCAT GATCAATGAG 
AAACAACTGC TGGCACTGCG TTGCGCCGTA CTCTACTGTT TCGAGTGCTT TCTTTATCGC 
AATGCCGATG GCCAGCGGGC GGTTGTACAG ACCCTGCTGC CCTCCAGTGC GTCCGATGTG 
AGTGCCCTTT CGACGGGACA ACTCCTTTGC ACCGGACTCT TCTCCACAGA TGCGCTTGCC 
AACTGGTTCT CCGCTGTGGC CCTAATGCAC TCACTCGTAG AGAATGTCGC CCTAAAGGAG 
GAACTACTGC GCGTACTGCT GGCCACTCCT GGTGGCCAAA AGCCTATCAC GCTACTCGAG 
CAGTGCACTA ATCTCATGCA GCAGGAACGC TACCGTTTGC AAAGCAAGGT GGGTCTGCTA 
ATGCTACTCA GCCTGTGGCT GGCGCATTGT CCAGGTGCGG TGAAGGCTTT GCTGGAAACG 
CAGGGCACCA TGGCCTACCT GACCGCTCAA CTTTGCTCCA ACGAGCACGA TGAACGAGAG 
TTCCTTGTCC AGGGCATGTG CGCATTCCTA ATGGGCCTGT GCATCCAGTT CAATGACAAC 
TCGCTGCCGG GGCAGAAGCG CGAGGACATC AGTCAGCTGA TCATCAAGCG AATTGGCCAG 
GAGAGCTTCT GTAGCAAACT AGCAGAAGTG TCGCGACATG AGGCCTACAG TCGGGCATGC 
AAGCAGGCCC AGATCCGTGC CAAATCCGCT GGGGAACTAC TGCTTGACTT CGAGTATTGC 
AAGCTGTACA AGGGACTGGA GGCGTTGATT GCCAAGCTGG TTAGCGGATT TGATGTAGAT 
GGCATTGAAC TGACCGAACT CACGTTGAGC TCGGAAGCTT CTGCATTGGT TTCCCAGTAC 
AAGGGGATTA TCCGCGGCAT GGATGCCCAG ATCCAAGCAC TGCAGCAATC CTCCAAAGAA 
CTGGAACAGG AGAATGCCGA GCTGAAGGAG AAACTTGGTG AGGAGCAGTC GCTCAAGGCG 
CAGCTCTTGG ACCAGAACAC GCTGCTTAAG GCACAGCTGG GAGCCTCGAC CGGTCAGGTG 
CAGTCCGCCC AAGGTGCGGA AGCCACGCCG CCCAACGAGG AGGAGCTAAA TGCCGCTCGC 
TACCAGGCCA ACATGTACTT TGCCGAAAAC ATTCGTCTAA CTAAGGAGCT GGAGACACTA 
CGTCAGCAGT TATCAGCCGA AAAACAGAGT GCAGATGCGG CGCAAGATTC CCTGGCGGCG 
ATGCAAAAGG ATCAAGAAGA TTTGCTCGAA CTGCTGGCCG ATCAGGAGGC AAAGCTAACG 
CGATACGAAG AAGCGGGCTC GACTAATACC CTTCCGACTT CCAATGTAGC TCCAAGCGTG 
CCGGCAGCTG GCGGAGGAAG CCCCATCCCA AGTGGCACCG CCAGCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284992.1
CDS	97..2607
Translation	MEFLKSGIKTVLGSTEPGQQPSAAETVEKLVDRVYSSTLLEDRRDACRALKALSRKYRIEVGAQGMPPLVQVLQNDGQDAEIISYALDTLCNVVTSEEFDEEADNPTVSVNVGEQFTEMFIKTPEHVTLVMGYLDEYDFRVRRAAIQLITSLISNKTRELQDLILVSPMGVSKLMDLLTDSREVIRNDVLLLLIELTKGNSNIQKIVAFENAFDRLFEIVREEGCSDGGIVVEDCLILLLNLLKNNSSNQQFFKEGSYIQRLSPMFELSQDAEEVGWSPQKVSNFHCLLQVVRALVTPSNQQQVVAACQRVMQKSRLLHALCEILMSSGVPADILTETINAVAEVVRGDRDNQDELGRVMAPSSPPRPAIVVLLMSMINEKQLLALRCAVLYCFECFLYRNADGQRAVVQTLLPSSASDVSALSTGQLLCTGLFSTDALANWFSAVALMHSLVENVALKEELLRVLLATPGGQKPITLLEQCTNLMQQERYRLQSKVGLLMLLSLWLAHCPGAVKALLETQGTMAYLTAQLCSNEHDEREFLVQGMCAFLMGLCIQFNDNSLPGQKREDISQLIIKRIGQESFCSKLAEVSRHEAYSRACKQAQIRAKSAGELLLDFEYCKLYKGLEALIAKLVSGFDVDGIELTELTLSSEASALVSQYKGIIRGMDAQIQALQQSSKELEQENAELKEKLGEEQSLKAQLLDQNTLLKAQLGASTGQVQSAQGAEATPPNEEELNAARYQANMYFAENIRLTKELETLRQQLSAEKQSADAAQDSLAAMQKDQEDLLELLADQEAKLTRYEEAGSTNTLPTSNVAPSVPAAGGGSPIPSGTASR

Target ORF information:

RefSeq Version	NM_001298063.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster p115, transcript variant B (p115), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298063.1

ORF Insert Sequence:

ATGGAGTTCC TGAAGAGTGG CATCAAAACG GTGCTGGGCA GCACGGAGCC GGGTCAGCAG 
CCGAGTGCCG CCGAGACGGT GGAGAAGCTG GTGGACCGTG TCTACTCCTC AACGCTCCTG 
GAGGATCGAC GGGATGCCTG CCGAGCGCTA AAAGCCCTGT CCCGCAAGTA CCGCATCGAG 
GTGGGCGCAC AGGGCATGCC TCCGCTGGTC CAGGTGCTCC AGAACGACGG ACAGGATGCA 
GAGATCATCA GCTATGCGCT GGACACACTG TGCAACGTGG TTACTAGCGA GGAGTTCGAC 
GAGGAGGCTG ATAATCCCAC CGTTTCGGTG AATGTGGGCG AGCAGTTCAC GGAGATGTTC 
ATCAAGACAC CGGAGCATGT AACCCTGGTA ATGGGCTATC TGGACGAATA CGATTTTCGA 
GTGCGTCGCG CTGCCATCCA ACTGATTACG TCCCTCATTT CGAACAAGAC GCGGGAGCTG 
CAGGATTTGA TTCTGGTCAG TCCGATGGGC GTTTCCAAGC TGATGGATCT GCTGACGGAC 
AGCCGCGAAG TGATTCGCAA CGATGTCCTT CTGCTGCTTA TCGAACTGAC CAAGGGCAAC 
TCAAACATCC AAAAGATTGT GGCCTTTGAG AATGCCTTTG ACCGCCTTTT TGAAATTGTC 
CGTGAGGAGG GCTGTTCGGA TGGCGGAATT GTTGTGGAGG ATTGCCTAAT TCTGCTATTG 
AATCTTCTCA AGAACAACTC CAGTAATCAG CAGTTCTTTA AGGAGGGCAG CTATATCCAG 
CGATTGTCGC CAATGTTTGA GCTCTCACAG GATGCGGAAG AGGTTGGCTG GTCGCCACAG 
AAGGTCTCAA ACTTCCATTG TCTGCTGCAG GTGGTGCGGG CACTGGTCAC GCCCAGTAAT 
CAACAGCAGG TGGTGGCCGC CTGCCAGCGG GTCATGCAAA AGTCTCGACT GCTCCATGCT 
CTTTGCGAGA TTCTGATGAG CAGTGGTGTC CCTGCGGATA TACTCACGGA AACCATCAAT 
GCAGTGGCCG AAGTGGTGCG CGGCGATCGG GACAACCAGG ATGAATTGGG AAGAGTTATG 
GCTCCGAGCA GTCCACCCAG ACCTGCCATC GTGGTCCTTC TTATGTCCAT GATCAATGAG 
AAACAACTGC TGGCACTGCG TTGCGCCGTA CTCTACTGTT TCGAGTGCTT TCTTTATCGC 
AATGCCGATG GCCAGCGGGC GGTTGTACAG ACCCTGCTGC CCTCCAGTGC GTCCGATGTG 
AGTGCCCTTT CGACGGGACA ACTCCTTTGC ACCGGACTCT TCTCCACAGA TGCGCTTGCC 
AACTGGTTCT CCGCTGTGGC CCTAATGCAC TCACTCGTAG AGAATGTCGC CCTAAAGGAG 
GAACTACTGC GCGTACTGCT GGCCACTCCT GGTGGCCAAA AGCCTATCAC GCTACTCGAG 
CAGTGCACTA ATCTCATGCA GCAGGAACGC TACCGTTTGC AAAGCAAGGT GGGTCTGCTA 
ATGCTACTCA GCCTGTGGCT GGCGCATTGT CCAGGTGCGG TGAAGGCTTT GCTGGAAACG 
CAGGGCACCA TGGCCTACCT GACCGCTCAA CTTTGCTCCA ACGAGCACGA TGAACGAGAG 
TTCCTTGTCC AGGGCATGTG CGCATTCCTA ATGGGCCTGT GCATCCAGTT CAATGACAAC 
TCGCTGCCGG GGCAGAAGCG CGAGGACATC AGTCAGCTGA TCATCAAGCG AATTGGCCAG 
GAGAGCTTCT GTAGCAAACT AGCAGAAGTG TCGCGACATG AGGCCTACAG TCGGGCATGC 
AAGCAGGCCC AGATCCGTGC CAAATCCGCT GGGGAACTAC TGCTTGACTT CGAGTATTGC 
AAGCTGTACA AGGGACTGGA GGCGTTGATT GCCAAGCTGG TTAGCGGATT TGATGTAGAT 
GGCATTGAAC TGACCGAACT CACGTTGAGC TCGGAAGCTT CTGCATTGGT TTCCCAGTAC 
AAGGGGATTA TCCGCGGCAT GGATGCCCAG ATCCAAGCAC TGCAGCAATC CTCCAAAGAA 
CTGGAACAGG AGAATGCCGA GCTGAAGGAG AAACTTGGTG AGGAGCAGTC GCTCAAGGCG 
CAGCTCTTGG ACCAGAACAC GCTGCTTAAG GCACAGCTGG GAGCCTCGAC CGGTCAGGTG 
CAGTCCGCCC AAGGTGCGGA AGCCACGCCG CCCAACGAGG AGGAGCTAAA TGCCGCTCGC 
TACCAGGCCA ACATGTACTT TGCCGAAAAC ATTCGTCTAA CTAAGGAGCT GGAGACACTA 
CGTCAGCAGT TATCAGCCGA AAAACAGAGT GCAGATGCGG CGCAAGATTC CCTGGCGGCG 
ATGCAAAAGG ATCAAGAAGA TTTGCTCGAA CTGCTGGCCG ATCAGGAGGC AAAGCTAACG 
CGATACGAAG AAGCGGGCTC GACTAATACC CTTCCGACTT CCAATGTAGC TCCAAGCGTG 
CCGGCAGCTG GCGGAGGAAG CCCCATCCCA AGTGGCACCG CCAGCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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p115 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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