Nek2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek2
Entrez Gene ID	31696
Full Name	CG17256 gene product from transcript CG17256-RA
Synonyms	CG17256,Dm Nek2,DmNek2,Dmel\CG17256,NEK2,dNek2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7C1-7C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_132187.3	NP_572415.1	Nek2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The APC/C degrades dNek2 upon synchronous G1 arrest prior to differentiation, which allows retinal differentiation through local suppression of Wg signaling.
Title: The APC/C Coordinates Retinal Differentiation with G1 Arrest through the Nek2-Dependent Modulation of Wingless Signaling.

APC/C(Fzr/Cdh1) negatively regulates Nek2, which negatively regulates Dsh, to ensure its proper stoichiometric requirement and localization during planar cell polarity establishment.
Title: APC/C

The following Nek2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01043	NM_132187.3 Latest version!	Drosophila melanogaster Nek2 (Nek2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nek2 ( NM_132187.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572415.1 Drosophila melanogaster Nek2 (Nek2), mRNA.

Price & Availability↑

CloneID	OFa01043
Clone ID Related Accession (Same CDS sequence)	NM_132187.3
Accession Version	NM_132187.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nek2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132187.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGAG AGGAATCTGC CGGCATGGAC TTTTCGCAAA AGACCCTGCA GGATTACGAG 
GTTTTGGCCG TGATGGGGAA TGGATCCTTC GGCACCTGCT ACAAGGTGCG CGACAAAAGC 
ACCGGTGAAT TGTTCGCCTG GAAGGGAATG AACTACGATG AACTGGACGA GGCCAAGTGC 
GACGCTTTGG TTTCGGAAAT CAGTGTTCTG CGGCAGCTCC AGCATCCTAA CATTGTGCAA 
TACTACCATC ATCTGGTGAA CCGAGAGGCC AAGTCGGTGT ACATTGTTAT GGAGTGCTGT 
GCCGGCGGCG ATCTCGCCCA GATTGTCCAG CGAGCGAGAT CCCAGAGGCA GCGGTTTGAG 
GAGCCCTACA TCTGGCGGGT GCTCTTCCAA CTTTGCCGGG CGCTTCAGGT GTGCCACAAC 
AAAATCCCCA ACGGAACCAT CTTGCATCGC GATATCAAGC CAGCAAATAT TTTCCTGGAT 
GCGGCCGGGA ATGCCAAACT GGGAGATTTC GGTCTGGCCA GAATGCTGCG ACGAGATCAG 
AGCTTTGCCG CCTCCTTTGT GGGCACTCCG CACTACATGA GTCCGGAGTT GGTGAAGGGC 
AGGAAATACG ATCGGAAGAG CGATGTCTGG GCCGTTGGTT GTTTGGTATA CGAGATGTGT 
GCCCTAAGAC CGCCGTTTCG CGGCAGAGCC TTTGACCAGT TGTCCGAGAA GATCGCCCAG 
GGTGAGTTCA GTCGGATTCC GGCAATCTAT AGTACTGATC TGCAGGAGAT CATCGCCTTC 
ATGCTGGCAG TGGATCATGA ACAGCGTCCT GGCATCGAGG TTATCATTCG GCATCCTCTG 
GTGGTGCGTA ATATTAGCGA GTTGGATGGC AAGTTCCCAA TTCTAGTTGA TTCTGGAGAG 
GATTTCTACA CACTGCCTTC GGGTGCCAGG CTTTTCGAGG ACGAAGAGGA AGATGGAGTT 
CATCCAGAGC TATCGAGCAC CATGTTTACG GAACAGTACA GCTTCAATGA AGGCTATGGG 
CAAAGGAGAC TCAGCGTTAC TGGTGTGTTT ACTCCAGATT TGCGAAGTGA ACTCTTCTAC 
TCCGCCAAGC GCAAAATCTT TCCTGCCAAG AAACTACAGC TGTCGGATCC CTCTCTTTAT 
GAAAGTATAA GGAGAGAGGA GCGAGCGGAG GAGAGGCAGG TGGAACTGGC GGAAGAGCGG 
CGCCGTAAAA AGGATCAGGA GCAGCAGAAA AGGGATCAGG AGCTTCTAAA GGAGGCCCCT 
AGCAGTCCTC GTGCTCTTAC CCAAAACATA TTCGATGAAG TACTAAAGAC CCGTCTCCAC 
GCCATTCGTG CCCAAGAATC CCTGTTGCAG CAGAAGCTCG AAGAGCTGCA GACCCGGGAG 
CAGGAGTTAC AGCTGGCCGA ACAGCGCGTG CAAACGCTGG AGCGGCAGAT GCAGGAAAAA 
CTTCTTCAGC AGGAGAAACA CACTTGTAGT TGCAGACAGC CGATAGCTCC GCCCATTCCT 
CCTAGGAAGC CAGCGAAATC GCACCATGAT GACACCTACT GCACTATCGA ACTCAACGAG 
ACGTCGCCCA CGGTGGCTAA ACTCAATTTA GCCACACTTC CGGCACCAAA ATCGCTAAAT 
ACTCTGCGTA AGGTTACCTT CAAGTCGCCC CAAAAGTTTG TTACCTATGG TATCGAGAAT 
ATGCCCCCAC CGGCGGTTAA ACCGGCAGTG AATCCTCCGC CCACTGGCGT TCCCATGCAA 
ATGAGCGTAT CCAGCAATGA TTCCGGTGAC AGCCAGGCCT CCTCCACCCG CAGGAAATCT 
ATACTCTCGC TTTTCGGGTT GAGTCGCGGA TCCAAGGCGA CATCCAAATC CTTGCCATCG 
GTCAACCAGG TAGCCCAAGC AGCTGCAACT CGCGTGCAGC GACCTCCTTT GGCCGTCAAG 
CCACCGATCA CGCAGGAACA GCCGGCAGAA GTTCCACCAG TGGCCAATCT CTGGACAAAG 
GAGCAGAAGA AACAAGCCTT CGAGCTGCTG GCGGCCATGA ATGCAGCCGA GAGGGAGGCA 
AGCGGAGGTG GAGTCGTTGG TTCTGGACCC GGAGGAGCAC GTCGCCAGCA TCTGCGGCAA 
TCGGTGAGGG AACGGAATTC CACGCTGCAG CGGAACCGCA TGCGGAGGTC TTTGGTGGTG 
GCCACTGGCC ATCAGAAGCT AACAACGCGC GACCAGATGG TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572415.1
CDS	148..2355
Translation	MSGEESAGMDFSQKTLQDYEVLAVMGNGSFGTCYKVRDKSTGELFAWKGMNYDELDEAKCDALVSEISVLRQLQHPNIVQYYHHLVNREAKSVYIVMECCAGGDLAQIVQRARSQRQRFEEPYIWRVLFQLCRALQVCHNKIPNGTILHRDIKPANIFLDAAGNAKLGDFGLARMLRRDQSFAASFVGTPHYMSPELVKGRKYDRKSDVWAVGCLVYEMCALRPPFRGRAFDQLSEKIAQGEFSRIPAIYSTDLQEIIAFMLAVDHEQRPGIEVIIRHPLVVRNISELDGKFPILVDSGEDFYTLPSGARLFEDEEEDGVHPELSSTMFTEQYSFNEGYGQRRLSVTGVFTPDLRSELFYSAKRKIFPAKKLQLSDPSLYESIRREERAEERQVELAEERRRKKDQEQQKRDQELLKEAPSSPRALTQNIFDEVLKTRLHAIRAQESLLQQKLEELQTREQELQLAEQRVQTLERQMQEKLLQQEKHTCSCRQPIAPPIPPRKPAKSHHDDTYCTIELNETSPTVAKLNLATLPAPKSLNTLRKVTFKSPQKFVTYGIENMPPPAVKPAVNPPPTGVPMQMSVSSNDSGDSQASSTRRKSILSLFGLSRGSKATSKSLPSVNQVAQAAATRVQRPPLAVKPPITQEQPAEVPPVANLWTKEQKKQAFELLAAMNAAEREASGGGVVGSGPGGARRQHLRQSVRERNSTLQRNRMRRSLVVATGHQKLTTRDQMVI

Target ORF information:

RefSeq Version	NM_132187.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nek2 (Nek2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132187.3

ORF Insert Sequence:

ATGAGCGGAG AGGAATCTGC CGGCATGGAC TTTTCGCAAA AGACCCTGCA GGATTACGAG 
GTTTTGGCCG TGATGGGGAA TGGATCCTTC GGCACCTGCT ACAAGGTGCG CGACAAAAGC 
ACCGGTGAAT TGTTCGCCTG GAAGGGAATG AACTACGATG AACTGGACGA GGCCAAGTGC 
GACGCTTTGG TTTCGGAAAT CAGTGTTCTG CGGCAGCTCC AGCATCCTAA CATTGTGCAA 
TACTACCATC ATCTGGTGAA CCGAGAGGCC AAGTCGGTGT ACATTGTTAT GGAGTGCTGT 
GCCGGCGGCG ATCTCGCCCA GATTGTCCAG CGAGCGAGAT CCCAGAGGCA GCGGTTTGAG 
GAGCCCTACA TCTGGCGGGT GCTCTTCCAA CTTTGCCGGG CGCTTCAGGT GTGCCACAAC 
AAAATCCCCA ACGGAACCAT CTTGCATCGC GATATCAAGC CAGCAAATAT TTTCCTGGAT 
GCGGCCGGGA ATGCCAAACT GGGAGATTTC GGTCTGGCCA GAATGCTGCG ACGAGATCAG 
AGCTTTGCCG CCTCCTTTGT GGGCACTCCG CACTACATGA GTCCGGAGTT GGTGAAGGGC 
AGGAAATACG ATCGGAAGAG CGATGTCTGG GCCGTTGGTT GTTTGGTATA CGAGATGTGT 
GCCCTAAGAC CGCCGTTTCG CGGCAGAGCC TTTGACCAGT TGTCCGAGAA GATCGCCCAG 
GGTGAGTTCA GTCGGATTCC GGCAATCTAT AGTACTGATC TGCAGGAGAT CATCGCCTTC 
ATGCTGGCAG TGGATCATGA ACAGCGTCCT GGCATCGAGG TTATCATTCG GCATCCTCTG 
GTGGTGCGTA ATATTAGCGA GTTGGATGGC AAGTTCCCAA TTCTAGTTGA TTCTGGAGAG 
GATTTCTACA CACTGCCTTC GGGTGCCAGG CTTTTCGAGG ACGAAGAGGA AGATGGAGTT 
CATCCAGAGC TATCGAGCAC CATGTTTACG GAACAGTACA GCTTCAATGA AGGCTATGGG 
CAAAGGAGAC TCAGCGTTAC TGGTGTGTTT ACTCCAGATT TGCGAAGTGA ACTCTTCTAC 
TCCGCCAAGC GCAAAATCTT TCCTGCCAAG AAACTACAGC TGTCGGATCC CTCTCTTTAT 
GAAAGTATAA GGAGAGAGGA GCGAGCGGAG GAGAGGCAGG TGGAACTGGC GGAAGAGCGG 
CGCCGTAAAA AGGATCAGGA GCAGCAGAAA AGGGATCAGG AGCTTCTAAA GGAGGCCCCT 
AGCAGTCCTC GTGCTCTTAC CCAAAACATA TTCGATGAAG TACTAAAGAC CCGTCTCCAC 
GCCATTCGTG CCCAAGAATC CCTGTTGCAG CAGAAGCTCG AAGAGCTGCA GACCCGGGAG 
CAGGAGTTAC AGCTGGCCGA ACAGCGCGTG CAAACGCTGG AGCGGCAGAT GCAGGAAAAA 
CTTCTTCAGC AGGAGAAACA CACTTGTAGT TGCAGACAGC CGATAGCTCC GCCCATTCCT 
CCTAGGAAGC CAGCGAAATC GCACCATGAT GACACCTACT GCACTATCGA ACTCAACGAG 
ACGTCGCCCA CGGTGGCTAA ACTCAATTTA GCCACACTTC CGGCACCAAA ATCGCTAAAT 
ACTCTGCGTA AGGTTACCTT CAAGTCGCCC CAAAAGTTTG TTACCTATGG TATCGAGAAT 
ATGCCCCCAC CGGCGGTTAA ACCGGCAGTG AATCCTCCGC CCACTGGCGT TCCCATGCAA 
ATGAGCGTAT CCAGCAATGA TTCCGGTGAC AGCCAGGCCT CCTCCACCCG CAGGAAATCT 
ATACTCTCGC TTTTCGGGTT GAGTCGCGGA TCCAAGGCGA CATCCAAATC CTTGCCATCG 
GTCAACCAGG TAGCCCAAGC AGCTGCAACT CGCGTGCAGC GACCTCCTTT GGCCGTCAAG 
CCACCGATCA CGCAGGAACA GCCGGCAGAA GTTCCACCAG TGGCCAATCT CTGGACAAAG 
GAGCAGAAGA AACAAGCCTT CGAGCTGCTG GCGGCCATGA ATGCAGCCGA GAGGGAGGCA 
AGCGGAGGTG GAGTCGTTGG TTCTGGACCC GGAGGAGCAC GTCGCCAGCA TCTGCGGCAA 
TCGGTGAGGG AACGGAATTC CACGCTGCAG CGGAACCGCA TGCGGAGGTC TTTGGTGGTG 
GCCACTGGCC ATCAGAAGCT AACAACGCGC GACCAGATGG TGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nek2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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