brk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	brk
Entrez Gene ID	31665
Full Name	brinker
Synonyms	BRK,Brk,CG9653,Dm brk,Dmel\CG9653,ssg-1
General protein information	Preferred Names brinker Names CG9653 gene product from transcript CG9653-RA CG9653-PA brk-PA small salivary glands-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7B1-7B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078514.4	NP_511069.3	brinker

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identify key roles for the Zelda and Zerknullt transcription factors in establishing the resulting expression domain, and find widespread binding of insulator proteins to the Mad and Brinker-bound genomic regions. Analysis of embryos lacking the BEAF-32 insulator protein shows reduced transcription of a peak BMP target gene and a reduction in the number of amnioserosa cells, the fate specified by peak BMP signaling.
Title: Regulation of the BMP Signaling-Responsive Transcriptional Network in the Drosophila Embryo.

although the recruitment of Gro is necessary and can be sufficient for Brk to make an almost morphologically wild-type fly, it is insufficient during oogenesis, where Brk must utilize CtBP and 3R to pattern the egg shell appropriately.
Title: Brinker possesses multiple mechanisms for repression because its primary co-repressor, Groucho, may be unavailable in some cell types.

here we investigate the cis-regulatory system controlling brinker (brk) expression in the Drosophila embryo.
Title: Autoregulatory feedback controls sequential action of cis-regulatory modules at the brinker locus.

Brk represses Myc expression and wing disc growth.
Title: Brk regulates wing disc growth in part via repression of Myc expression.

This work found that mutating the silencer elements in the brk locus results in an expansion of the brk expression domain. However, repression could still be observed in regions of maximal Dpp levels.
Title: Manipulating the sensitivity of signal-induced repression: quantification and consequences of altered brinker gradients.

The following brk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01012	NM_078514.4 Latest version!	Drosophila melanogaster brinker (brk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brk ( NM_078514.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511069.3 Drosophila melanogaster brinker (brk), mRNA.

Price & Availability↑

CloneID	OFa01012
Clone ID Related Accession (Same CDS sequence)	NM_078514.4
Accession Version	NM_078514.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brinker
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078514.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAGCA GCAGCGAACA GTTGAACGGA TCGGGAGCTT TGAATTTCAA GCGGCCCAAG 
GATTCTTCGG AGAATGCCAC AAACAGCCAC ACAAATAATG GCAATTCTTC GGGCAGCCCC 
AAAATGGGAA GTCGTCGGAT TTTCACGCCC CACTTTAAGC TGCAAGTCCT CGAATCATAC 
AGGAATGATA ATGATTGCAA GGGCAATCAA CGGGCCACGG CCAGGAAATA CAACATTCAC 
CGCCGGCAAA TCCAAAAATG GTTGCAATGC GAGTCAAATT TGCGATCATC GGTGGCCAAC 
AATCAGCAAC AGCAGCAGCA GCAGCAACAG CAACAGCAGC AACAACAGCA GCAGCAGCAA 
CTACTCCCAC AGCAATCGGT ATCGCCGACA CCGGCGGTCA AGGTGTTCCA TCAGCTGAGC 
CATCCGCTGG TGCACCAGTT GCATCACCAT CACGCCGCCG CGGTGGGTCA TCATCATCAT 
CATGCCGCCC ACCATCATGC CGCCCATCAT CATCACGCTG CAGCAGCAGC GGCGGCAGCA 
GCAGCGGCAG CAGCAGCAGC AGCAGCCCAC CATCATGCCG CCCACCATTT GCTGGCAGCC 
AATGGCATGG TTCCACACCC CCTGGCCGCC CATCCCCACT TGCATGTCCC GGTGGCAATG 
CATCCTCAAA TGCAGCATCA GAAGGAGCAA CAGCAGCAGC AGCAGCAGTT GCAGCAGGAG 
CAGCAGCAAC AGGATCAGCA GCAGTCACAG CAGGAGACAC CTGCAACCAT TGCTACCAAT 
GGCAGCAATC AGGGATCCTC GAATGTCCTT TCGGCGGCCA AAATAGCCGC TGTTGTTGCC 
GCCGCCATGG CCACCAGCAA TGGCAATCCC ACACCGACAG CAACAATCCC CGCCAGCAGC 
AGCAGCAGCA GCAACACATT GCCGAGCAGC AATAACAGTA GTTGCCACAA CAGCAGCAGC 
AGCAGCAATA GCTGCAACAG CAACCAAGTG CCAATACAAG TGCCAATCCT GAGCGGATCC 
CCTGGGTCCA CATCGTCAGC CAGTCACATT CCACATGTGC CCTTTGCCTA CCATCACAAT 
CTGCATGGCT ATTTGGAGAA TCGACTGGAG GCGGTTGCCA CACCAGCCCC CATGGACCTC 
TCTCTCGGAT CCTCTGCCCG CCGTCAAATG CAGTTGCACG AAAAGGATCC ATCTGGTGTG 
GATCTCACTT TCCGCAAACG TAAGGTCATC ACTAGTCCGA TGCAGCCGGA TAAGATTAGT 
AAGCTGGAGG AGGTGATCAA GAAGGAGCCC GAAACGGAAA CGGAGAACGA GGATGTGGAG 
GTGGACGTGG AGACGGAGCA GCCGGAGGAG CACAAGCTGC CCTCTAAACA GGTCAAGCTA 
TTTAAGCCCT ATTTGCTGGA CGATGATGAG GAGCAGGATC ATCATCATCA TCACCATCAC 
CATCGGCAGG AGGATTTGGA TGAGGGAGCT GCCGAGGAGG AGCAGGACGA CGAGGAAGAG 
TCCCGCTATG CCGACGATGA TGAGGTGGAC TCCAAGGAGG CGGCCGACAA GAAACAGCGT 
CGCCTCAAGA AAAAGCCATC GGCAATCAAC GAACAAAGGG AACCCATCAT CTGGAGCAAC 
CATCCGTATC CCGGTGGCTG TGTATCGCCC GGCAGTTCGA TCACCAGCAG CTTTCAGTGC 
CCCACCAGTA TGCAACAGCA GCAGACATTT CCCGTCGCCG GCGGGAGTCC TAATCAGCAA 
TTTCAGGACA ACTGCAGCTC CAGCAAAGCC ACCACGCCCC TCAGCCCATT CTCGGCGCCC 
GCCCTCTCGC CCACCGGCTT CTGTTGCCCC AAAGGATCGC CCGTTTCCGG TTACGAGAGC 
AGCTCATCCA CCTACAGCGA CAGCGGCAGC AACTACAGCC TCAATCTCCA GCTGCATGCC 
GTCTACAACG ACAATCTGAT GTACATGCAA CAGCAGCAGC AGCAGCAGCA ACAGCAGCAT 
CACCTGCAAC ATCAGCAGCA CCTGCAGCGC TGGCTGGATC AGGAGTCCTT GGCCACTGCG 
AGGACATCAT CCGTCAACCG ACCACTGATT CTCGTGGCGG ATACTGCGCC CACAAACCTG 
ACCCTGGTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511069.3
CDS	535..2649
Translation	MDSSSEQLNGSGALNFKRPKDSSENATNSHTNNGNSSGSPKMGSRRIFTPHFKLQVLESYRNDNDCKGNQRATARKYNIHRRQIQKWLQCESNLRSSVANNQQQQQQQQQQQQQQQQQQQLLPQQSVSPTPAVKVFHQLSHPLVHQLHHHHAAAVGHHHHHAAHHHAAHHHHAAAAAAAAAAAAAAAAAHHHAAHHLLAANGMVPHPLAAHPHLHVPVAMHPQMQHQKEQQQQQQQLQQEQQQQDQQQSQQETPATIATNGSNQGSSNVLSAAKIAAVVAAAMATSNGNPTPTATIPASSSSSSNTLPSSNNSSCHNSSSSSNSCNSNQVPIQVPILSGSPGSTSSASHIPHVPFAYHHNLHGYLENRLEAVATPAPMDLSLGSSARRQMQLHEKDPSGVDLTFRKRKVITSPMQPDKISKLEEVIKKEPETETENEDVEVDVETEQPEEHKLPSKQVKLFKPYLLDDDEEQDHHHHHHHHRQEDLDEGAAEEEQDDEEESRYADDDEVDSKEAADKKQRRLKKKPSAINEQREPIIWSNHPYPGGCVSPGSSITSSFQCPTSMQQQQTFPVAGGSPNQQFQDNCSSSKATTPLSPFSAPALSPTGFCCPKGSPVSGYESSSSTYSDSGSNYSLNLQLHAVYNDNLMYMQQQQQQQQQQHHLQHQQHLQRWLDQESLATARTSSVNRPLILVADTAPTNLTLVA

Target ORF information:

RefSeq Version	NM_078514.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brinker (brk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078514.4

ORF Insert Sequence:

ATGGATAGCA GCAGCGAACA GTTGAACGGA TCGGGAGCTT TGAATTTCAA GCGGCCCAAG 
GATTCTTCGG AGAATGCCAC AAACAGCCAC ACAAATAATG GCAATTCTTC GGGCAGCCCC 
AAAATGGGAA GTCGTCGGAT TTTCACGCCC CACTTTAAGC TGCAAGTCCT CGAATCATAC 
AGGAATGATA ATGATTGCAA GGGCAATCAA CGGGCCACGG CCAGGAAATA CAACATTCAC 
CGCCGGCAAA TCCAAAAATG GTTGCAATGC GAGTCAAATT TGCGATCATC GGTGGCCAAC 
AATCAGCAAC AGCAGCAGCA GCAGCAACAG CAACAGCAGC AACAACAGCA GCAGCAGCAA 
CTACTCCCAC AGCAATCGGT ATCGCCGACA CCGGCGGTCA AGGTGTTCCA TCAGCTGAGC 
CATCCGCTGG TGCACCAGTT GCATCACCAT CACGCCGCCG CGGTGGGTCA TCATCATCAT 
CATGCCGCCC ACCATCATGC CGCCCATCAT CATCACGCTG CAGCAGCAGC GGCGGCAGCA 
GCAGCGGCAG CAGCAGCAGC AGCAGCCCAC CATCATGCCG CCCACCATTT GCTGGCAGCC 
AATGGCATGG TTCCACACCC CCTGGCCGCC CATCCCCACT TGCATGTCCC GGTGGCAATG 
CATCCTCAAA TGCAGCATCA GAAGGAGCAA CAGCAGCAGC AGCAGCAGTT GCAGCAGGAG 
CAGCAGCAAC AGGATCAGCA GCAGTCACAG CAGGAGACAC CTGCAACCAT TGCTACCAAT 
GGCAGCAATC AGGGATCCTC GAATGTCCTT TCGGCGGCCA AAATAGCCGC TGTTGTTGCC 
GCCGCCATGG CCACCAGCAA TGGCAATCCC ACACCGACAG CAACAATCCC CGCCAGCAGC 
AGCAGCAGCA GCAACACATT GCCGAGCAGC AATAACAGTA GTTGCCACAA CAGCAGCAGC 
AGCAGCAATA GCTGCAACAG CAACCAAGTG CCAATACAAG TGCCAATCCT GAGCGGATCC 
CCTGGGTCCA CATCGTCAGC CAGTCACATT CCACATGTGC CCTTTGCCTA CCATCACAAT 
CTGCATGGCT ATTTGGAGAA TCGACTGGAG GCGGTTGCCA CACCAGCCCC CATGGACCTC 
TCTCTCGGAT CCTCTGCCCG CCGTCAAATG CAGTTGCACG AAAAGGATCC ATCTGGTGTG 
GATCTCACTT TCCGCAAACG TAAGGTCATC ACTAGTCCGA TGCAGCCGGA TAAGATTAGT 
AAGCTGGAGG AGGTGATCAA GAAGGAGCCC GAAACGGAAA CGGAGAACGA GGATGTGGAG 
GTGGACGTGG AGACGGAGCA GCCGGAGGAG CACAAGCTGC CCTCTAAACA GGTCAAGCTA 
TTTAAGCCCT ATTTGCTGGA CGATGATGAG GAGCAGGATC ATCATCATCA TCACCATCAC 
CATCGGCAGG AGGATTTGGA TGAGGGAGCT GCCGAGGAGG AGCAGGACGA CGAGGAAGAG 
TCCCGCTATG CCGACGATGA TGAGGTGGAC TCCAAGGAGG CGGCCGACAA GAAACAGCGT 
CGCCTCAAGA AAAAGCCATC GGCAATCAAC GAACAAAGGG AACCCATCAT CTGGAGCAAC 
CATCCGTATC CCGGTGGCTG TGTATCGCCC GGCAGTTCGA TCACCAGCAG CTTTCAGTGC 
CCCACCAGTA TGCAACAGCA GCAGACATTT CCCGTCGCCG GCGGGAGTCC TAATCAGCAA 
TTTCAGGACA ACTGCAGCTC CAGCAAAGCC ACCACGCCCC TCAGCCCATT CTCGGCGCCC 
GCCCTCTCGC CCACCGGCTT CTGTTGCCCC AAAGGATCGC CCGTTTCCGG TTACGAGAGC 
AGCTCATCCA CCTACAGCGA CAGCGGCAGC AACTACAGCC TCAATCTCCA GCTGCATGCC 
GTCTACAACG ACAATCTGAT GTACATGCAA CAGCAGCAGC AGCAGCAGCA ACAGCAGCAT 
CACCTGCAAC ATCAGCAGCA CCTGCAGCGC TGGCTGGATC AGGAGTCCTT GGCCACTGCG 
AGGACATCAT CCGTCAACCG ACCACTGATT CTCGTGGCGG ATACTGCGCC CACAAACCTG 
ACCCTGGTGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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brk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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