Inx2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Inx2
Entrez Gene ID	31646
Full Name	Innexin 2
Synonyms	CG4590,D-inx-2,Dm-inx,Dm-inx2,Dmel\CG4590,FBpp0291074,INX-2,Ix2,inx-2,inx2,kropf,l(1)G0035,l(1)G0036,l(1)G0043,l(1)G0059,l(1)G0118,l(1)G0157,l(1)G0317,l(1)G0364,prp33
General protein information	Preferred Names Innexin 2 Names CG4590 gene product from transcript CG4590-RD CG4590-PA CG4590-PB CG4590-PC CG4590-PD Innexin-2 Inx2-PA Inx2-PB Inx2-PC Inx2-PD innexin innexin 2 innexin2 pas related protein 33
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 6E4-6E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169213.2	NP_001162684.1	innexin 2, isoform C
NM_001272372.1	NP_001259301.1	innexin 2, isoform D
NM_132147.4	NP_572375.1	innexin 2, isoform A
NM_167104.3	NP_727150.1	innexin 2, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Inx2	NP_727150.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001488	XP_001238517.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Inx2 mediated calcium flux in the follicle cells stimulates endocytosis by altering Dynamin (Shibire) distribution which is critical for careful calibration of STAT activation and, thus for border cell specification.
Title: A Gap Junction Protein, Inx2, Modulates Calcium Flux to Specify Border Cell Fate during Drosophila oogenesis.

inx2 is required for the transcriptional activation of dpp and punt in the eye disc.
Title: Drosophila eye size is determined by Innexin 2-dependent Decapentaplegic signalling.

Downregulation of either ogre or inx2 selectively in glia, by targeted expression of RNA interference transgenes, leads to a significant reduction in the size of the larval nervous system and behavioural defects in surviving adults.
Title: Innexins Ogre and Inx2 are required in glial cells for normal postembryonic development of the Drosophila central nervous system.

Data propose that Inx3 is a critical factor for dorsal closure and that it mediates the stability of Inx1, 2 and DE-cadherin by forming a complex.
Title: Innexin 3, a new gene required for dorsal closure in Drosophila embryo.

Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila.
Title: Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila.

The following Inx2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Inx2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00992	NM_167104.3 Latest version!	Drosophila melanogaster innexin 2, transcript variant B (Inx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00992	NM_132147.4 Latest version!	Drosophila melanogaster innexin 2, transcript variant A (Inx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00992	NM_001169213.2 Latest version!	Drosophila melanogaster innexin 2, transcript variant C (Inx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00992	NM_001272372.1 Latest version!	Drosophila melanogaster innexin 2, transcript variant D (Inx2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Inx2 ( NM_001169213.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162684.1 Drosophila melanogaster innexin 2, transcript variant C (Inx2), mRNA.

Price & Availability↑

CloneID	OFa00992
Clone ID Related Accession (Same CDS sequence)	NM_001169213.2 , NM_001272372.1 , NM_132147.4 , NM_167104.3
Accession Version	NM_001169213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	innexin 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169213.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162684.1
CDS	458..1561
Translation	MFDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIPLGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWKSWEGGRLKMLVMDLNSPIVNDECKNDRKKILVDYFIGNLNRHNFYAFRFFVCEALNFVNVIGQIYFVDFFLDGEFSTYGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLIYRIAVVAGPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDLSREMSGDEHSAHKRPFDA

Target ORF information:

RefSeq Version	NM_001169213.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster innexin 2, transcript variant C (Inx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169213.2

ORF Insert Sequence:

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Inx2 ( NM_001272372.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259301.1 Drosophila melanogaster innexin 2, transcript variant D (Inx2), mRNA.

Price & Availability↑

CloneID	OFa00992
Clone ID Related Accession (Same CDS sequence)	NM_001169213.2 , NM_001272372.1 , NM_132147.4 , NM_167104.3
Accession Version	NM_001272372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	innexin 2, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259301.1
CDS	244..1347
Translation	MFDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIPLGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWKSWEGGRLKMLVMDLNSPIVNDECKNDRKKILVDYFIGNLNRHNFYAFRFFVCEALNFVNVIGQIYFVDFFLDGEFSTYGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLIYRIAVVAGPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDLSREMSGDEHSAHKRPFDA

Target ORF information:

RefSeq Version	NM_001272372.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster innexin 2, transcript variant D (Inx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272372.1

ORF Insert Sequence:

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Inx2 ( NM_132147.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572375.1 Drosophila melanogaster innexin 2, transcript variant A (Inx2), mRNA.

Price & Availability↑

CloneID	OFa00992
Clone ID Related Accession (Same CDS sequence)	NM_001169213.2 , NM_001272372.1 , NM_132147.4 , NM_167104.3
Accession Version	NM_132147.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	innexin 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132147.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572375.1
CDS	458..1561
Translation	MFDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIPLGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWKSWEGGRLKMLVMDLNSPIVNDECKNDRKKILVDYFIGNLNRHNFYAFRFFVCEALNFVNVIGQIYFVDFFLDGEFSTYGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLIYRIAVVAGPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDLSREMSGDEHSAHKRPFDA

Target ORF information:

RefSeq Version	NM_132147.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster innexin 2, transcript variant A (Inx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132147.4

ORF Insert Sequence:

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Inx2 ( NM_167104.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727150.1 Drosophila melanogaster innexin 2, transcript variant B (Inx2), mRNA.

Price & Availability↑

CloneID	OFa00992
Clone ID Related Accession (Same CDS sequence)	NM_001169213.2 , NM_001272372.1 , NM_132147.4 , NM_167104.3
Accession Version	NM_167104.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	innexin 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167104.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727150.1
CDS	244..1347
Translation	MFDVFGSVKGLLKIDQVCIDNNVFRMHYKATVIILIAFSLLVTSRQYIGDPIDCIVDEIPLGVMDTYCWIYSTFTVPERLTGITGRDVVQPGVGSHVEGEDEVKYHKYYQWVCFVLFFQAILFYVPRYLWKSWEGGRLKMLVMDLNSPIVNDECKNDRKKILVDYFIGNLNRHNFYAFRFFVCEALNFVNVIGQIYFVDFFLDGEFSTYGSDVLKFTELEPDERIDPMARVFPKVTKCTFHKYGPSGSVQTHDGLCVLPLNIVNEKIYVFLWFWFIILSIMSGISLIYRIAVVAGPKLRHLLLRARSRLAESEEVELVANKCNIGDWFLLYQLGKNIDPLIYKEVISDLSREMSGDEHSAHKRPFDA

Target ORF information:

RefSeq Version	NM_167104.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster innexin 2, transcript variant B (Inx2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167104.3

ORF Insert Sequence:

ATGTTTGATG TCTTTGGGTC CGTCAAGGGC CTGCTGAAGA TCGACCAGGT GTGCATCGAC 
AACAATGTCT TTCGCATGCA CTACAAGGCC ACGGTGATCA TACTGATCGC CTTCTCCCTC 
CTGGTGACCT CGCGCCAATA CATCGGTGAC CCCATCGATT GTATTGTGGA CGAGATCCCA 
CTGGGCGTGA TGGACACCTA CTGCTGGATC TACTCTACGT TTACCGTGCC AGAGAGGCTA 
ACGGGCATCA CCGGACGCGA TGTGGTGCAG CCCGGCGTGG GCTCCCATGT GGAGGGCGAG 
GACGAGGTGA AGTACCACAA GTACTACCAG TGGGTGTGCT TCGTCCTCTT CTTCCAGGCC 
ATCCTGTTCT ACGTACCGCG CTATCTGTGG AAGTCCTGGG AAGGCGGACG CCTCAAGATG 
CTGGTCATGG ATCTCAACAG CCCTATTGTG AACGATGAGT GCAAGAACGA TCGCAAAAAG 
ATCCTGGTCG ACTACTTCAT TGGCAACCTG AACCGCCACA ATTTCTACGC CTTCCGATTC 
TTCGTGTGCG AAGCCCTGAA CTTTGTGAAT GTGATTGGAC AGATCTACTT TGTGGACTTC 
TTCCTCGACG GCGAGTTCAG CACCTACGGC AGTGATGTCT TGAAGTTCAC TGAGCTGGAG 
CCGGATGAGC GCATTGATCC CATGGCGCGG GTCTTTCCGA AGGTCACCAA ATGCACGTTC 
CACAAATACG GTCCATCCGG CAGTGTGCAG ACCCACGACG GTCTGTGCGT GCTGCCCCTG 
AACATTGTCA ACGAAAAGAT CTACGTGTTC CTGTGGTTCT GGTTCATCAT CCTGAGCATC 
ATGTCGGGAA TATCGCTTAT CTACAGAATC GCTGTTGTGG CGGGTCCCAA GCTCCGCCAT 
CTCCTACTCC GAGCCCGTTC CCGTTTGGCT GAAAGCGAGG AGGTCGAACT GGTGGCCAAC 
AAGTGCAACA TCGGCGATTG GTTCCTGCTC TATCAGCTGG GCAAGAACAT CGATCCGCTC 
ATCTACAAGG AGGTGATCTC GGACTTGTCC CGCGAAATGA GCGGCGATGA GCATAGCGCC 
CACAAGCGGC CCTTCGACGC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Inx2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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