shf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	shf
Entrez Gene ID	31617
Full Name	shifted
Synonyms	CG3134,CG3135,CT10514,D-Wif,DmWIF,DmWif,Dmel\CG3135,Shf,shf-ov
General protein information	Preferred Names shifted Names CG3135 gene product from transcript CG3135-RC CG3135-PA CG3135-PB CG3135-PC CG3135-PD WNT inhibitory factor shf-PA shf-PB shf-PC shf-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 6C13-6D1\|1-17.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_132121.3	NP_572349.1	shifted, isoform A
NM_001298036.1	NP_001284965.1	shifted, isoform D
NM_001298034.1	NP_001284963.1	shifted, isoform B
NM_001298035.1	NP_001284964.1	shifted, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WIF1	NP_001186536.1
Rattus norvegicus (Norway rat)	Wif1	NP_446190.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000117	XP_311028.4
Danio rerio (zebrafish)	wif1	NP_571304.1
Homo sapiens (human)	WIF1	NP_009122.2
Pan troglodytes (chimpanzee)	WIF1	XP_001163469.1
	WIF1	XP_001116962.1
Canis lupus familiaris (dog)	WIF1	XP_538269.2
Mus musculus (house mouse)	Wif1	NP_036045.1
Drosophila melanogaster (fruit fly)	shf	NP_572349.1
Xenopus tropicalis (tropical clawed frog)	wif1	NP_001106386.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of balancing between hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif
Title: Balancing Hedgehog, a retention and release equilibrium given by Dally, Ihog, Boi and shifted/DmWif.

We show that production of this PGC attractant depends upon shf as well as a second hh pathway gene g gamma1.
Title: The hedgehog pathway gene shifted functions together with the hmgcr-dependent isoprenoid biosynthetic pathway to orchestrate germ cell migration.

study concludes that the WIF domain is responsible for the divergence between Drosophila Shf and human Wif-1, channeling the recognition either toward Hh or Wg signal, whereas the EGF repeats give the protein the ability to interact with glypicans
Title: The WIF domain of the human and Drosophila Wif-1 secreted factors confers specificity for Wnt or Hedgehog.

Shifted is a secreted protein that acts over a long distance and is required for the normal accumulation of Hh protein and its movement in the wing.
Title: Shifted, the Drosophila ortholog of Wnt inhibitory factor-1, controls the distribution and movement of Hedgehog.

Shf is the ortholog of the human Wnt inhibitory factor (WIF), a secreted antagonist of the Wingless pathway. Shf is required for Hh stability and for lipid-modified Hh diffusion.
Title: The Drosophila ortholog of the human Wnt inhibitor factor Shifted controls the diffusion of lipid-modified Hedgehog.

The following shf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00966	NM_132121.3 Latest version!	Drosophila melanogaster shifted (shf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21390	NM_001298035.1 Latest version!	Drosophila melanogaster shifted (shf), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21391	NM_001298036.1 Latest version!	Drosophila melanogaster shifted (shf), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa21392	NM_001298034.1 Latest version!	Drosophila melanogaster shifted (shf), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shf ( NM_132121.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572349.1 Drosophila melanogaster shifted (shf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00966
Clone ID Related Accession (Same CDS sequence)	NM_132121.3
Accession Version	NM_132121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shifted, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132121.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGGTGTGTA TGATATAGAC GCTTCCAACC CTACTTCACT AACAACTTTG 
CAAGAATGCA GCTTGAAGTG CGGCAAGAAC GGGTACTGCA ACGAGCACCA CATCTGCAAG 
TGCAACGTGG GCTACACGGG CCAATACTGC GAGACGGCCT TCTGTTTTCC GCAGTGTCTC 
AATGGCGGTA ACTGCACGGC GCCGTCCGTC TGCACCTGTC CGGAGGGCTA CCAGGGCACG 
CAGTGCGAAG GTGGTATTTG CAAGGACAAG TGCTTGAATG GCGGCAAGTG TATACAGAAG 
GACAAGTGCC AGTGCTCCAA GGGTTACTAC GGCCTGCGTT GCGAGTACTC CAAGTGTGTG 
ATCCCCTGCA AGAACGAGGG ACGGTGCATC GGGAACAACC TGTGCCGCTG CCCCAACGGA 
CTACGAGGCG ATCACTGCGA GATCGGGCGC AAGCAGCGCT CCATCTGCAA GTGTCGCAAT 
GGCACCTGCG TCTCCCACAA GCACTGCAAG TGCCATCCGG GATTCTATGG ACGCCACTGC 
AACGGTCGCA AGCGACGACA TGTCCACCGA AACGATGACT CCAAGTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572349.1
CDS	261..1631
Translation	MTHQGIGCLVKWLYLVLIVHTLLCIGQLECRQQHHNRNNNNNNRRADSSSSEEGHGNTSDGLDNFADQDASFVGHGHQPRRGQRKKQQGGGGGGSGGGGGNGGGGGSRHNRNEESGISLWINEQQLKMLTALYFPQGYSERLYAIHNSRVTNDLRDTTLYNFLVIPSEVNYVNFTWKSGRRKYFYDFDRLQTMDESILKAPTLSIRKSGRIPQEQKNFSIFLPCTGNSSGTASFNVGLKIQTRHNKPLSGTPIRLNFKKECAHRGVYDIDASNPTSLTTLQECSLKCGKNGYCNEHHICKCNVGYTGQYCETAFCFPQCLNGGNCTAPSVCTCPEGYQGTQCEGGICKDKCLNGGKCIQKDKCQCSKGYYGLRCEYSKCVIPCKNEGRCIGNNLCRCPNGLRGDHCEIGRKQRSICKCRNGTCVSHKHCKCHPGFYGRHCNGRKRRHVHRNDDSKF

Target ORF information:

RefSeq Version	NM_132121.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shifted (shf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132121.3

ORF Insert Sequence:

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGGTGTGTA TGATATAGAC GCTTCCAACC CTACTTCACT AACAACTTTG 
CAAGAATGCA GCTTGAAGTG CGGCAAGAAC GGGTACTGCA ACGAGCACCA CATCTGCAAG 
TGCAACGTGG GCTACACGGG CCAATACTGC GAGACGGCCT TCTGTTTTCC GCAGTGTCTC 
AATGGCGGTA ACTGCACGGC GCCGTCCGTC TGCACCTGTC CGGAGGGCTA CCAGGGCACG 
CAGTGCGAAG GTGGTATTTG CAAGGACAAG TGCTTGAATG GCGGCAAGTG TATACAGAAG 
GACAAGTGCC AGTGCTCCAA GGGTTACTAC GGCCTGCGTT GCGAGTACTC CAAGTGTGTG 
ATCCCCTGCA AGAACGAGGG ACGGTGCATC GGGAACAACC TGTGCCGCTG CCCCAACGGA 
CTACGAGGCG ATCACTGCGA GATCGGGCGC AAGCAGCGCT CCATCTGCAA GTGTCGCAAT 
GGCACCTGCG TCTCCCACAA GCACTGCAAG TGCCATCCGG GATTCTATGG ACGCCACTGC 
AACGGTCGCA AGCGACGACA TGTCCACCGA AACGATGACT CCAAGTTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shf ( NM_001298035.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284964.1 Drosophila melanogaster shifted (shf), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa21390
Clone ID Related Accession (Same CDS sequence)	NM_001298035.1
Accession Version	NM_001298035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shifted, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGAATGCAG CTTGAAGTGC GGCAAGAACG GGTACTGCAA CGAGCACCAC 
ATCTGCAAGT GCAACGTGGG CTACACGGGC CAATACTGCG AGACGGCCTT CTGTTTTCCG 
CAGTGTCTCA ATGGCGGTAA CTGCACGGCG CCGTCCGTCT GCACCTGTCC GGAGGGCTAC 
CAGGGCACGC AGTGCGAAGG TGGTATTTGC AAGGACAAGT GCTTGAATGG CGGCAAGTGT 
ATACAGAAGG ACAAGTGCCA GTGCTCCAAG GGTTACTACG GCCTGCGTTG CGAGTACTCC 
AAGTGTGTGA TCCCCTGCAA GAACGAGGGA CGGTGCATCG GGAACAACCT GTGCCGCTGC 
CCCAACGGAC TACGAGGCGA TCACTGCGAG ATCGGGCGCA AGCAGCGCTC CATCTGCAAG 
TGTCGCAATG GCACCTGCGT CTCCCACAAG CACTGCAAGT GCCATCCGGG ATTCTATGGA 
CGCCACTGCA ACGGTCGCAA GCGACGACAT GTCCACCGAA ACGATGACTC CAAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284964.1
CDS	261..1580
Translation	MTHQGIGCLVKWLYLVLIVHTLLCIGQLECRQQHHNRNNNNNNRRADSSSSEEGHGNTSDGLDNFADQDASFVGHGHQPRRGQRKKQQGGGGGGSGGGGGNGGGGGSRHNRNEESGISLWINEQQLKMLTALYFPQGYSERLYAIHNSRVTNDLRDTTLYNFLVIPSEVNYVNFTWKSGRRKYFYDFDRLQTMDESILKAPTLSIRKSGRIPQEQKNFSIFLPCTGNSSGTASFNVGLKIQTRHNKPLSGTPIRLNFKKECAHRECSLKCGKNGYCNEHHICKCNVGYTGQYCETAFCFPQCLNGGNCTAPSVCTCPEGYQGTQCEGGICKDKCLNGGKCIQKDKCQCSKGYYGLRCEYSKCVIPCKNEGRCIGNNLCRCPNGLRGDHCEIGRKQRSICKCRNGTCVSHKHCKCHPGFYGRHCNGRKRRHVHRNDDSKF

Target ORF information:

RefSeq Version	NM_001298035.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shifted (shf), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298035.1

ORF Insert Sequence:

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGAATGCAG CTTGAAGTGC GGCAAGAACG GGTACTGCAA CGAGCACCAC 
ATCTGCAAGT GCAACGTGGG CTACACGGGC CAATACTGCG AGACGGCCTT CTGTTTTCCG 
CAGTGTCTCA ATGGCGGTAA CTGCACGGCG CCGTCCGTCT GCACCTGTCC GGAGGGCTAC 
CAGGGCACGC AGTGCGAAGG TGGTATTTGC AAGGACAAGT GCTTGAATGG CGGCAAGTGT 
ATACAGAAGG ACAAGTGCCA GTGCTCCAAG GGTTACTACG GCCTGCGTTG CGAGTACTCC 
AAGTGTGTGA TCCCCTGCAA GAACGAGGGA CGGTGCATCG GGAACAACCT GTGCCGCTGC 
CCCAACGGAC TACGAGGCGA TCACTGCGAG ATCGGGCGCA AGCAGCGCTC CATCTGCAAG 
TGTCGCAATG GCACCTGCGT CTCCCACAAG CACTGCAAGT GCCATCCGGG ATTCTATGGA 
CGCCACTGCA ACGGTCGCAA GCGACGACAT GTCCACCGAA ACGATGACTC CAAGTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shf ( NM_001298036.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284965.1 Drosophila melanogaster shifted (shf), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa21391
Clone ID Related Accession (Same CDS sequence)	NM_001298036.1
Accession Version	NM_001298036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shifted, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGCTCCCGA TCCAGAATGC AGCTTGAAGT GCGGCAAGAA CGGGTACTGC 
AACGAGCACC ACATCTGCAA GTGCAACGTG GGCTACACGG GCCAATACTG CGAGACGGCC 
TTCTGTTTTC CGCAGTGTCT CAATGGCGGT AACTGCACGG CGCCGTCCGT CTGCACCTGT 
CCGGAGGGCT ACCAGGGCAC GCAGTGCGAA GGTGGTATTT GCAAGGACAA GTGCTTGAAT 
GGCGGCAAGT GTATACAGAA GGACAAGTGC CAGTGCTCCA AGGGTTACTA CGGCCTGCGT 
TGCGAGTACT CCAAGTGTGT GATCCCCTGC AAGAACGAGG GACGGTGCAT CGGGAACAAC 
CTGTGCCGCT GCCCCAACGG ACTACGAGGC GATCACTGCG AGATCGGGCG CAAGCAGCGC 
TCCATCTGCA AGTGTCGCAA TGGCACCTGC GTCTCCCACA AGCACTGCAA GTGCCATCCG 
GGATTCTATG GACGCCACTG CAACGGTCGC AAGCGACGAC ATGTCCACCG AAACGATGAC 
TCCAAGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284965.1
CDS	261..1592
Translation	MTHQGIGCLVKWLYLVLIVHTLLCIGQLECRQQHHNRNNNNNNRRADSSSSEEGHGNTSDGLDNFADQDASFVGHGHQPRRGQRKKQQGGGGGGSGGGGGNGGGGGSRHNRNEESGISLWINEQQLKMLTALYFPQGYSERLYAIHNSRVTNDLRDTTLYNFLVIPSEVNYVNFTWKSGRRKYFYDFDRLQTMDESILKAPTLSIRKSGRIPQEQKNFSIFLPCTGNSSGTASFNVGLKIQTRHNKPLSGTPIRLNFKKECAHRAPDPECSLKCGKNGYCNEHHICKCNVGYTGQYCETAFCFPQCLNGGNCTAPSVCTCPEGYQGTQCEGGICKDKCLNGGKCIQKDKCQCSKGYYGLRCEYSKCVIPCKNEGRCIGNNLCRCPNGLRGDHCEIGRKQRSICKCRNGTCVSHKHCKCHPGFYGRHCNGRKRRHVHRNDDSKF

Target ORF information:

RefSeq Version	NM_001298036.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shifted (shf), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298036.1

ORF Insert Sequence:

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGCTCCCGA TCCAGAATGC AGCTTGAAGT GCGGCAAGAA CGGGTACTGC 
AACGAGCACC ACATCTGCAA GTGCAACGTG GGCTACACGG GCCAATACTG CGAGACGGCC 
TTCTGTTTTC CGCAGTGTCT CAATGGCGGT AACTGCACGG CGCCGTCCGT CTGCACCTGT 
CCGGAGGGCT ACCAGGGCAC GCAGTGCGAA GGTGGTATTT GCAAGGACAA GTGCTTGAAT 
GGCGGCAAGT GTATACAGAA GGACAAGTGC CAGTGCTCCA AGGGTTACTA CGGCCTGCGT 
TGCGAGTACT CCAAGTGTGT GATCCCCTGC AAGAACGAGG GACGGTGCAT CGGGAACAAC 
CTGTGCCGCT GCCCCAACGG ACTACGAGGC GATCACTGCG AGATCGGGCG CAAGCAGCGC 
TCCATCTGCA AGTGTCGCAA TGGCACCTGC GTCTCCCACA AGCACTGCAA GTGCCATCCG 
GGATTCTATG GACGCCACTG CAACGGTCGC AAGCGACGAC ATGTCCACCG AAACGATGAC 
TCCAAGTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shf ( NM_001298034.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284963.1 Drosophila melanogaster shifted (shf), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa21392
Clone ID Related Accession (Same CDS sequence)	NM_001298034.1
Accession Version	NM_001298034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shifted, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGGTGTGTA TGATATAGAC GCTTCCAACC CTACTTCACT AACAACTTTG 
CAAGCTCCCG ATCCAGAATG CAGCTTGAAG TGCGGCAAGA ACGGGTACTG CAACGAGCAC 
CACATCTGCA AGTGCAACGT GGGCTACACG GGCCAATACT GCGAGACGGC CTTCTGTTTT 
CCGCAGTGTC TCAATGGCGG TAACTGCACG GCGCCGTCCG TCTGCACCTG TCCGGAGGGC 
TACCAGGGCA CGCAGTGCGA AGGTGGTATT TGCAAGGACA AGTGCTTGAA TGGCGGCAAG 
TGTATACAGA AGGACAAGTG CCAGTGCTCC AAGGGTTACT ACGGCCTGCG TTGCGAGTAC 
TCCAAGTGTG TGATCCCCTG CAAGAACGAG GGACGGTGCA TCGGGAACAA CCTGTGCCGC 
TGCCCCAACG GACTACGAGG CGATCACTGC GAGATCGGGC GCAAGCAGCG CTCCATCTGC 
AAGTGTCGCA ATGGCACCTG CGTCTCCCAC AAGCACTGCA AGTGCCATCC GGGATTCTAT 
GGACGCCACT GCAACGGTCG CAAGCGACGA CATGTCCACC GAAACGATGA CTCCAAGTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284963.1
CDS	261..1643
Translation	MTHQGIGCLVKWLYLVLIVHTLLCIGQLECRQQHHNRNNNNNNRRADSSSSEEGHGNTSDGLDNFADQDASFVGHGHQPRRGQRKKQQGGGGGGSGGGGGNGGGGGSRHNRNEESGISLWINEQQLKMLTALYFPQGYSERLYAIHNSRVTNDLRDTTLYNFLVIPSEVNYVNFTWKSGRRKYFYDFDRLQTMDESILKAPTLSIRKSGRIPQEQKNFSIFLPCTGNSSGTASFNVGLKIQTRHNKPLSGTPIRLNFKKECAHRGVYDIDASNPTSLTTLQAPDPECSLKCGKNGYCNEHHICKCNVGYTGQYCETAFCFPQCLNGGNCTAPSVCTCPEGYQGTQCEGGICKDKCLNGGKCIQKDKCQCSKGYYGLRCEYSKCVIPCKNEGRCIGNNLCRCPNGLRGDHCEIGRKQRSICKCRNGTCVSHKHCKCHPGFYGRHCNGRKRRHVHRNDDSKF

Target ORF information:

RefSeq Version	NM_001298034.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shifted (shf), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298034.1

ORF Insert Sequence:

ATGACACATC AGGGCATCGG CTGCCTGGTC AAGTGGTTAT ATCTGGTGCT GATCGTGCAC 
ACGCTCCTCT GCATCGGCCA GCTCGAGTGT CGCCAGCAGC ACCACAATCG GAATAACAAT 
AATAATAACA GAAGAGCGGA CTCGTCCAGC TCAGAGGAGG GCCACGGCAA CACCAGCGAT 
GGCCTGGACA ACTTCGCAGA TCAGGACGCC AGCTTCGTTG GCCATGGACA CCAGCCGCGT 
CGGGGTCAAC GAAAGAAACA GCAGGGAGGT GGAGGAGGAG GCAGCGGCGG CGGCGGCGGA 
AACGGAGGAG GCGGTGGAAG CCGGCACAAT CGCAACGAGG AGAGCGGCAT CTCGTTGTGG 
ATCAATGAGC AGCAGCTCAA AATGCTTACA GCGCTCTACT TCCCACAGGG CTACTCGGAG 
CGCCTGTACG CCATTCACAA CAGCCGGGTG ACCAATGATC TGAGGGACAC GACGCTGTAC 
AACTTCCTGG TAATCCCATC GGAGGTCAAC TACGTTAACT TCACATGGAA GTCGGGGCGC 
CGCAAATACT TCTACGACTT TGACCGCCTG CAGACCATGG ATGAAAGTAT CTTGAAGGCG 
CCAACGCTGT CCATTCGCAA GAGCGGACGC ATTCCGCAGG AGCAGAAAAA TTTTAGCATA 
TTCCTGCCCT GCACCGGCAA CAGCTCCGGC ACCGCGTCCT TCAACGTCGG CCTCAAGATC 
CAGACGCGCC ACAACAAGCC GCTCTCGGGC ACACCCATTC GCCTCAACTT CAAAAAGGAA 
TGCGCCCACA GAGGTGTGTA TGATATAGAC GCTTCCAACC CTACTTCACT AACAACTTTG 
CAAGCTCCCG ATCCAGAATG CAGCTTGAAG TGCGGCAAGA ACGGGTACTG CAACGAGCAC 
CACATCTGCA AGTGCAACGT GGGCTACACG GGCCAATACT GCGAGACGGC CTTCTGTTTT 
CCGCAGTGTC TCAATGGCGG TAACTGCACG GCGCCGTCCG TCTGCACCTG TCCGGAGGGC 
TACCAGGGCA CGCAGTGCGA AGGTGGTATT TGCAAGGACA AGTGCTTGAA TGGCGGCAAG 
TGTATACAGA AGGACAAGTG CCAGTGCTCC AAGGGTTACT ACGGCCTGCG TTGCGAGTAC 
TCCAAGTGTG TGATCCCCTG CAAGAACGAG GGACGGTGCA TCGGGAACAA CCTGTGCCGC 
TGCCCCAACG GACTACGAGG CGATCACTGC GAGATCGGGC GCAAGCAGCG CTCCATCTGC 
AAGTGTCGCA ATGGCACCTG CGTCTCCCAC AAGCACTGCA AGTGCCATCC GGGATTCTAT 
GGACGCCACT GCAACGGTCG CAAGCGACGA CATGTCCACC GAAACGATGA CTCCAAGTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

shf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報