Pat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pat1
Entrez Gene ID	31593
Full Name	Protein interacting with APP tail-1
Synonyms	CG10695,Dmel\CG10695,PAT1,pat1
General protein information	Preferred Names Protein interacting with APP tail-1 Names CG10695 gene product from transcript CG10695-RA CG10695-PA CG10695-PB CG10695-PC Pat1-PA Pat1-PB Pat1-PC protein interacting with APP tail-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 6C1-6C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272356.2	NP_001259285.1	protein interacting with APP tail-1, isoform B
NM_132100.5	NP_572328.1	protein interacting with APP tail-1, isoform A
NM_001272357.2	NP_001259286.1	protein interacting with APP tail-1, isoform C

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	APPBP2	XP_001136833.2
Mus musculus (house mouse)	Appbp2	NP_080101.1
Gallus gallus (chicken)	APPBP2	NP_001025894.1
Homo sapiens (human)	APPBP2	NP_006371.2
	APPBP2	XP_001107544.2
Canis lupus familiaris (dog)	APPBP2	XP_537708.2
Bos taurus (cattle)	APPBP2	XP_002695688.2
Rattus norvegicus (Norway rat)	Appbp2	NP_001094439.1
Danio rerio (zebrafish)	appbp2	NP_997798.1
Drosophila melanogaster (fruit fly)	Pat1	NP_572328.1
Xenopus tropicalis (tropical clawed frog)	appbp2	XP_002933870.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

suggests that the role of PAT1 in cargo transport in the cell is linked to PAT1 function as a positive regulator of Kinesin motility.
Title: Drosophila PAT1 is required for Kinesin-1 to transport cargo and to maximize its motility.

The following Pat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00952	NM_132100.5 Latest version!	Drosophila melanogaster protein interacting with APP tail-1, transcript variant A (Pat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00952	NM_001272357.2 Latest version!	Drosophila melanogaster protein interacting with APP tail-1, transcript variant C (Pat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00953	NM_001272356.2 Latest version!	Drosophila melanogaster protein interacting with APP tail-1, transcript variant B (Pat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pat1 ( NM_132100.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572328.1 Drosophila melanogaster protein interacting with APP tail-1, transcript variant A (Pat1), mRNA.

Price & Availability↑

CloneID	OFa00952
Clone ID Related Accession (Same CDS sequence)	NM_132100.5 , NM_001272357.2
Accession Version	NM_132100.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein interacting with APP tail-1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132100.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCC AACAGCTCGT GGTCTTGATC CCCAAAATAA ATCCCAACTC CCCGAAGACC 
CTATACGACC TGTCGCTGCA GGCCCTCATC GGGAGTATGA ACACCAAGAT ACCGGCGCTG 
GACAGGATCA GTCGACTGGC TGAGCTGCCA CGCAACTTGC TCATCGATGT TTACGAAATG 
ATGTCACAGC AGGAGTCCTT GAAGGACACG CTGCTGGAGG AGCTGGCCCA GCTTGAGGTA 
TTCGCTCGAC TCGTTCGCTA TCCATTTGCT CGCAGTCAAT TGCTGCGCAT AATGGCCTCA 
CTAATGGCCA GCCAAAAGAT GCTGGCCAAA AGACTCAGCG AGAACTATGT GTCGCGCTAT 
GAGGTGGTGG CCGGTCACGA AGAAGAGGAA CTGGAGTCGA GTATGGATAC AGTTGAGGAT 
CGCTCAACGG AGTTCAGTTC ACTGTATACC TACGATTCCG AATCTACAAA TGCAGACAGC 
ATCAGTGATT TTAATGACGA ATTCAGTGCT GATATCGGAA GCATAGTAGG TCTCCCACAC 
CGGAGAGCAA CACAGGAGGA ACTCGATGCG ATCAATGATT CTGGCCTCGA GGAGCTGTCC 
GCCGTTGAGC TGCAGGTCAT AGATCTGGGC CTGCGCCTCG GATCGTTCCT CTCGGAGGCT 
GGATGGATGC AGGAGAGCAT TACCGTGCTG GCATGCCTTA ATGTAAGACT GAAGGATCTG 
CCGACCCACA AACACTGGTT GCAGTTTCGA CTCGACTGCC TGCAGCGCCT TTTGTATGCC 
GAATCGGCCC ATTGCAACTT CAAGGAGGCA GAGAAAACCT ACGCGGAATT GATGGGTCTG 
AACAAATGGC TCAATAAGAG CGTGCCAAAC CAGCTGGTGG CCATAACCTA CAGTCAGATT 
TCGGCCATGT ACTTTGCCCG CAATGAGTAC AAGAACAGTC ATTTGTGGAG CGGTCTCGCC 
ATGCGATTTT TAAAAGGATA TGCGAATCCA CGCACCATCA TCGATGTTCT GCGCCAGGCG 
GCGAAGGCCT GTGTCGTTAA GCGGGACTTT GCCCGGGCCA ATCTGCTCAT CTGCCAGGCG 
GTGCGACGCG CACGCGAGTA CTTTGGCCCA ACGCACCAGA AATACGGCGA TGCACTGCTG 
GACTACGGAT TTTTCCTACT CAACGTGGAC TCGGTGTTCC AGTCTGTAAA TATATACAAA 
GAAGCACTGG CCGTGCGTCG TGGTATTTTT GGGAATATGA ACTTTCACGT GGCCATTGCC 
CATGAGGATT TGTCGTATGC CTACTACGTT CACGAGTACA GCACCGGTGA CTTTAGCTGT 
GCCCAGGATC ACGTGGACAA GGCTGTGAAT ATAATGCAGC ATCTGGTGCC AAGCAATCAT 
CTAATGTTGG CATCGGCGAA GCGCGTAAAG GCACTCCTGC TGGAGGAGAT TGCGCTGGAC 
AAGATGGCTG ATGGCATAGA TGAGGAGGAT CTGCTGCTTC AATCCGAGGA GCTGCACAAT 
TTCGCCCTGC TTCTCTCGCT GCAAGTGTTT GGCGAGGTGA ATGTACAGAC GGCGAAGCAC 
TATGGAAATC TGGGGCGGTT ATACCAAACG ATGAATCGTT TCGAAGAGGC GGAACGAATG 
CACAAAAAGG CGATCAAAAT TAAGTCGGAA CTGCTGGGTC ACTTTGACTA CGAGGTTGGC 
CTGTCCATTG GGCATTTGGC CTCGCTGTAC AATTACCAGA TGAAGAAATA CCGTGATGCC 
GAACAGCTTT ACATGCGCAG CATTGATATA AGCCTGCGTC TGTTTGGGAA CTCATACTCT 
GGCCTGGAGT ACGATTATTT GGGTCTGTGC CACGTCTACG AAACATTGCA TAACTTTGAG 
AAGTATTTGA AGTATGCGCA CAAGCTGGAA AACTGGCAGA TGTTGCGTGG CCAAAACCTC 
ACACAAAATA AGTCCAGCTA TCCCGCCATC GAGGTGGACT ATTCTATCGA GGAGGTCAAG 
GCCAAGTACT TCAGCATGTG CGCCTCGAAA AAAAGCGCAC ACCCCTCGGT GCACGAGGAG 
GACACGGTGA ACGAAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572328.1
CDS	369..2429
Translation	MAGQQLVVLIPKINPNSPKTLYDLSLQALIGSMNTKIPALDRISRLAELPRNLLIDVYEMMSQQESLKDTLLEELAQLEVFARLVRYPFARSQLLRIMASLMASQKMLAKRLSENYVSRYEVVAGHEEEELESSMDTVEDRSTEFSSLYTYDSESTNADSISDFNDEFSADIGSIVGLPHRRATQEELDAINDSGLEELSAVELQVIDLGLRLGSFLSEAGWMQESITVLACLNVRLKDLPTHKHWLQFRLDCLQRLLYAESAHCNFKEAEKTYAELMGLNKWLNKSVPNQLVAITYSQISAMYFARNEYKNSHLWSGLAMRFLKGYANPRTIIDVLRQAAKACVVKRDFARANLLICQAVRRAREYFGPTHQKYGDALLDYGFFLLNVDSVFQSVNIYKEALAVRRGIFGNMNFHVAIAHEDLSYAYYVHEYSTGDFSCAQDHVDKAVNIMQHLVPSNHLMLASAKRVKALLLEEIALDKMADGIDEEDLLLQSEELHNFALLLSLQVFGEVNVQTAKHYGNLGRLYQTMNRFEEAERMHKKAIKIKSELLGHFDYEVGLSIGHLASLYNYQMKKYRDAEQLYMRSIDISLRLFGNSYSGLEYDYLGLCHVYETLHNFEKYLKYAHKLENWQMLRGQNLTQNKSSYPAIEVDYSIEEVKAKYFSMCASKKSAHPSVHEEDTVNEN

Target ORF information:

RefSeq Version	NM_132100.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein interacting with APP tail-1, transcript variant A (Pat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132100.5

ORF Insert Sequence:

ATGGCTGGCC AACAGCTCGT GGTCTTGATC CCCAAAATAA ATCCCAACTC CCCGAAGACC 
CTATACGACC TGTCGCTGCA GGCCCTCATC GGGAGTATGA ACACCAAGAT ACCGGCGCTG 
GACAGGATCA GTCGACTGGC TGAGCTGCCA CGCAACTTGC TCATCGATGT TTACGAAATG 
ATGTCACAGC AGGAGTCCTT GAAGGACACG CTGCTGGAGG AGCTGGCCCA GCTTGAGGTA 
TTCGCTCGAC TCGTTCGCTA TCCATTTGCT CGCAGTCAAT TGCTGCGCAT AATGGCCTCA 
CTAATGGCCA GCCAAAAGAT GCTGGCCAAA AGACTCAGCG AGAACTATGT GTCGCGCTAT 
GAGGTGGTGG CCGGTCACGA AGAAGAGGAA CTGGAGTCGA GTATGGATAC AGTTGAGGAT 
CGCTCAACGG AGTTCAGTTC ACTGTATACC TACGATTCCG AATCTACAAA TGCAGACAGC 
ATCAGTGATT TTAATGACGA ATTCAGTGCT GATATCGGAA GCATAGTAGG TCTCCCACAC 
CGGAGAGCAA CACAGGAGGA ACTCGATGCG ATCAATGATT CTGGCCTCGA GGAGCTGTCC 
GCCGTTGAGC TGCAGGTCAT AGATCTGGGC CTGCGCCTCG GATCGTTCCT CTCGGAGGCT 
GGATGGATGC AGGAGAGCAT TACCGTGCTG GCATGCCTTA ATGTAAGACT GAAGGATCTG 
CCGACCCACA AACACTGGTT GCAGTTTCGA CTCGACTGCC TGCAGCGCCT TTTGTATGCC 
GAATCGGCCC ATTGCAACTT CAAGGAGGCA GAGAAAACCT ACGCGGAATT GATGGGTCTG 
AACAAATGGC TCAATAAGAG CGTGCCAAAC CAGCTGGTGG CCATAACCTA CAGTCAGATT 
TCGGCCATGT ACTTTGCCCG CAATGAGTAC AAGAACAGTC ATTTGTGGAG CGGTCTCGCC 
ATGCGATTTT TAAAAGGATA TGCGAATCCA CGCACCATCA TCGATGTTCT GCGCCAGGCG 
GCGAAGGCCT GTGTCGTTAA GCGGGACTTT GCCCGGGCCA ATCTGCTCAT CTGCCAGGCG 
GTGCGACGCG CACGCGAGTA CTTTGGCCCA ACGCACCAGA AATACGGCGA TGCACTGCTG 
GACTACGGAT TTTTCCTACT CAACGTGGAC TCGGTGTTCC AGTCTGTAAA TATATACAAA 
GAAGCACTGG CCGTGCGTCG TGGTATTTTT GGGAATATGA ACTTTCACGT GGCCATTGCC 
CATGAGGATT TGTCGTATGC CTACTACGTT CACGAGTACA GCACCGGTGA CTTTAGCTGT 
GCCCAGGATC ACGTGGACAA GGCTGTGAAT ATAATGCAGC ATCTGGTGCC AAGCAATCAT 
CTAATGTTGG CATCGGCGAA GCGCGTAAAG GCACTCCTGC TGGAGGAGAT TGCGCTGGAC 
AAGATGGCTG ATGGCATAGA TGAGGAGGAT CTGCTGCTTC AATCCGAGGA GCTGCACAAT 
TTCGCCCTGC TTCTCTCGCT GCAAGTGTTT GGCGAGGTGA ATGTACAGAC GGCGAAGCAC 
TATGGAAATC TGGGGCGGTT ATACCAAACG ATGAATCGTT TCGAAGAGGC GGAACGAATG 
CACAAAAAGG CGATCAAAAT TAAGTCGGAA CTGCTGGGTC ACTTTGACTA CGAGGTTGGC 
CTGTCCATTG GGCATTTGGC CTCGCTGTAC AATTACCAGA TGAAGAAATA CCGTGATGCC 
GAACAGCTTT ACATGCGCAG CATTGATATA AGCCTGCGTC TGTTTGGGAA CTCATACTCT 
GGCCTGGAGT ACGATTATTT GGGTCTGTGC CACGTCTACG AAACATTGCA TAACTTTGAG 
AAGTATTTGA AGTATGCGCA CAAGCTGGAA AACTGGCAGA TGTTGCGTGG CCAAAACCTC 
ACACAAAATA AGTCCAGCTA TCCCGCCATC GAGGTGGACT ATTCTATCGA GGAGGTCAAG 
GCCAAGTACT TCAGCATGTG CGCCTCGAAA AAAAGCGCAC ACCCCTCGGT GCACGAGGAG 
GACACGGTGA ACGAAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pat1 ( NM_001272357.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259286.1 Drosophila melanogaster protein interacting with APP tail-1, transcript variant C (Pat1), mRNA.

Price & Availability↑

CloneID	OFa00952
Clone ID Related Accession (Same CDS sequence)	NM_132100.5 , NM_001272357.2
Accession Version	NM_001272357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein interacting with APP tail-1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272357.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCC AACAGCTCGT GGTCTTGATC CCCAAAATAA ATCCCAACTC CCCGAAGACC 
CTATACGACC TGTCGCTGCA GGCCCTCATC GGGAGTATGA ACACCAAGAT ACCGGCGCTG 
GACAGGATCA GTCGACTGGC TGAGCTGCCA CGCAACTTGC TCATCGATGT TTACGAAATG 
ATGTCACAGC AGGAGTCCTT GAAGGACACG CTGCTGGAGG AGCTGGCCCA GCTTGAGGTA 
TTCGCTCGAC TCGTTCGCTA TCCATTTGCT CGCAGTCAAT TGCTGCGCAT AATGGCCTCA 
CTAATGGCCA GCCAAAAGAT GCTGGCCAAA AGACTCAGCG AGAACTATGT GTCGCGCTAT 
GAGGTGGTGG CCGGTCACGA AGAAGAGGAA CTGGAGTCGA GTATGGATAC AGTTGAGGAT 
CGCTCAACGG AGTTCAGTTC ACTGTATACC TACGATTCCG AATCTACAAA TGCAGACAGC 
ATCAGTGATT TTAATGACGA ATTCAGTGCT GATATCGGAA GCATAGTAGG TCTCCCACAC 
CGGAGAGCAA CACAGGAGGA ACTCGATGCG ATCAATGATT CTGGCCTCGA GGAGCTGTCC 
GCCGTTGAGC TGCAGGTCAT AGATCTGGGC CTGCGCCTCG GATCGTTCCT CTCGGAGGCT 
GGATGGATGC AGGAGAGCAT TACCGTGCTG GCATGCCTTA ATGTAAGACT GAAGGATCTG 
CCGACCCACA AACACTGGTT GCAGTTTCGA CTCGACTGCC TGCAGCGCCT TTTGTATGCC 
GAATCGGCCC ATTGCAACTT CAAGGAGGCA GAGAAAACCT ACGCGGAATT GATGGGTCTG 
AACAAATGGC TCAATAAGAG CGTGCCAAAC CAGCTGGTGG CCATAACCTA CAGTCAGATT 
TCGGCCATGT ACTTTGCCCG CAATGAGTAC AAGAACAGTC ATTTGTGGAG CGGTCTCGCC 
ATGCGATTTT TAAAAGGATA TGCGAATCCA CGCACCATCA TCGATGTTCT GCGCCAGGCG 
GCGAAGGCCT GTGTCGTTAA GCGGGACTTT GCCCGGGCCA ATCTGCTCAT CTGCCAGGCG 
GTGCGACGCG CACGCGAGTA CTTTGGCCCA ACGCACCAGA AATACGGCGA TGCACTGCTG 
GACTACGGAT TTTTCCTACT CAACGTGGAC TCGGTGTTCC AGTCTGTAAA TATATACAAA 
GAAGCACTGG CCGTGCGTCG TGGTATTTTT GGGAATATGA ACTTTCACGT GGCCATTGCC 
CATGAGGATT TGTCGTATGC CTACTACGTT CACGAGTACA GCACCGGTGA CTTTAGCTGT 
GCCCAGGATC ACGTGGACAA GGCTGTGAAT ATAATGCAGC ATCTGGTGCC AAGCAATCAT 
CTAATGTTGG CATCGGCGAA GCGCGTAAAG GCACTCCTGC TGGAGGAGAT TGCGCTGGAC 
AAGATGGCTG ATGGCATAGA TGAGGAGGAT CTGCTGCTTC AATCCGAGGA GCTGCACAAT 
TTCGCCCTGC TTCTCTCGCT GCAAGTGTTT GGCGAGGTGA ATGTACAGAC GGCGAAGCAC 
TATGGAAATC TGGGGCGGTT ATACCAAACG ATGAATCGTT TCGAAGAGGC GGAACGAATG 
CACAAAAAGG CGATCAAAAT TAAGTCGGAA CTGCTGGGTC ACTTTGACTA CGAGGTTGGC 
CTGTCCATTG GGCATTTGGC CTCGCTGTAC AATTACCAGA TGAAGAAATA CCGTGATGCC 
GAACAGCTTT ACATGCGCAG CATTGATATA AGCCTGCGTC TGTTTGGGAA CTCATACTCT 
GGCCTGGAGT ACGATTATTT GGGTCTGTGC CACGTCTACG AAACATTGCA TAACTTTGAG 
AAGTATTTGA AGTATGCGCA CAAGCTGGAA AACTGGCAGA TGTTGCGTGG CCAAAACCTC 
ACACAAAATA AGTCCAGCTA TCCCGCCATC GAGGTGGACT ATTCTATCGA GGAGGTCAAG 
GCCAAGTACT TCAGCATGTG CGCCTCGAAA AAAAGCGCAC ACCCCTCGGT GCACGAGGAG 
GACACGGTGA ACGAAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259286.1
CDS	369..2429
Translation	MAGQQLVVLIPKINPNSPKTLYDLSLQALIGSMNTKIPALDRISRLAELPRNLLIDVYEMMSQQESLKDTLLEELAQLEVFARLVRYPFARSQLLRIMASLMASQKMLAKRLSENYVSRYEVVAGHEEEELESSMDTVEDRSTEFSSLYTYDSESTNADSISDFNDEFSADIGSIVGLPHRRATQEELDAINDSGLEELSAVELQVIDLGLRLGSFLSEAGWMQESITVLACLNVRLKDLPTHKHWLQFRLDCLQRLLYAESAHCNFKEAEKTYAELMGLNKWLNKSVPNQLVAITYSQISAMYFARNEYKNSHLWSGLAMRFLKGYANPRTIIDVLRQAAKACVVKRDFARANLLICQAVRRAREYFGPTHQKYGDALLDYGFFLLNVDSVFQSVNIYKEALAVRRGIFGNMNFHVAIAHEDLSYAYYVHEYSTGDFSCAQDHVDKAVNIMQHLVPSNHLMLASAKRVKALLLEEIALDKMADGIDEEDLLLQSEELHNFALLLSLQVFGEVNVQTAKHYGNLGRLYQTMNRFEEAERMHKKAIKIKSELLGHFDYEVGLSIGHLASLYNYQMKKYRDAEQLYMRSIDISLRLFGNSYSGLEYDYLGLCHVYETLHNFEKYLKYAHKLENWQMLRGQNLTQNKSSYPAIEVDYSIEEVKAKYFSMCASKKSAHPSVHEEDTVNEN

Target ORF information:

RefSeq Version	NM_001272357.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein interacting with APP tail-1, transcript variant C (Pat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272357.2

ORF Insert Sequence:

ATGGCTGGCC AACAGCTCGT GGTCTTGATC CCCAAAATAA ATCCCAACTC CCCGAAGACC 
CTATACGACC TGTCGCTGCA GGCCCTCATC GGGAGTATGA ACACCAAGAT ACCGGCGCTG 
GACAGGATCA GTCGACTGGC TGAGCTGCCA CGCAACTTGC TCATCGATGT TTACGAAATG 
ATGTCACAGC AGGAGTCCTT GAAGGACACG CTGCTGGAGG AGCTGGCCCA GCTTGAGGTA 
TTCGCTCGAC TCGTTCGCTA TCCATTTGCT CGCAGTCAAT TGCTGCGCAT AATGGCCTCA 
CTAATGGCCA GCCAAAAGAT GCTGGCCAAA AGACTCAGCG AGAACTATGT GTCGCGCTAT 
GAGGTGGTGG CCGGTCACGA AGAAGAGGAA CTGGAGTCGA GTATGGATAC AGTTGAGGAT 
CGCTCAACGG AGTTCAGTTC ACTGTATACC TACGATTCCG AATCTACAAA TGCAGACAGC 
ATCAGTGATT TTAATGACGA ATTCAGTGCT GATATCGGAA GCATAGTAGG TCTCCCACAC 
CGGAGAGCAA CACAGGAGGA ACTCGATGCG ATCAATGATT CTGGCCTCGA GGAGCTGTCC 
GCCGTTGAGC TGCAGGTCAT AGATCTGGGC CTGCGCCTCG GATCGTTCCT CTCGGAGGCT 
GGATGGATGC AGGAGAGCAT TACCGTGCTG GCATGCCTTA ATGTAAGACT GAAGGATCTG 
CCGACCCACA AACACTGGTT GCAGTTTCGA CTCGACTGCC TGCAGCGCCT TTTGTATGCC 
GAATCGGCCC ATTGCAACTT CAAGGAGGCA GAGAAAACCT ACGCGGAATT GATGGGTCTG 
AACAAATGGC TCAATAAGAG CGTGCCAAAC CAGCTGGTGG CCATAACCTA CAGTCAGATT 
TCGGCCATGT ACTTTGCCCG CAATGAGTAC AAGAACAGTC ATTTGTGGAG CGGTCTCGCC 
ATGCGATTTT TAAAAGGATA TGCGAATCCA CGCACCATCA TCGATGTTCT GCGCCAGGCG 
GCGAAGGCCT GTGTCGTTAA GCGGGACTTT GCCCGGGCCA ATCTGCTCAT CTGCCAGGCG 
GTGCGACGCG CACGCGAGTA CTTTGGCCCA ACGCACCAGA AATACGGCGA TGCACTGCTG 
GACTACGGAT TTTTCCTACT CAACGTGGAC TCGGTGTTCC AGTCTGTAAA TATATACAAA 
GAAGCACTGG CCGTGCGTCG TGGTATTTTT GGGAATATGA ACTTTCACGT GGCCATTGCC 
CATGAGGATT TGTCGTATGC CTACTACGTT CACGAGTACA GCACCGGTGA CTTTAGCTGT 
GCCCAGGATC ACGTGGACAA GGCTGTGAAT ATAATGCAGC ATCTGGTGCC AAGCAATCAT 
CTAATGTTGG CATCGGCGAA GCGCGTAAAG GCACTCCTGC TGGAGGAGAT TGCGCTGGAC 
AAGATGGCTG ATGGCATAGA TGAGGAGGAT CTGCTGCTTC AATCCGAGGA GCTGCACAAT 
TTCGCCCTGC TTCTCTCGCT GCAAGTGTTT GGCGAGGTGA ATGTACAGAC GGCGAAGCAC 
TATGGAAATC TGGGGCGGTT ATACCAAACG ATGAATCGTT TCGAAGAGGC GGAACGAATG 
CACAAAAAGG CGATCAAAAT TAAGTCGGAA CTGCTGGGTC ACTTTGACTA CGAGGTTGGC 
CTGTCCATTG GGCATTTGGC CTCGCTGTAC AATTACCAGA TGAAGAAATA CCGTGATGCC 
GAACAGCTTT ACATGCGCAG CATTGATATA AGCCTGCGTC TGTTTGGGAA CTCATACTCT 
GGCCTGGAGT ACGATTATTT GGGTCTGTGC CACGTCTACG AAACATTGCA TAACTTTGAG 
AAGTATTTGA AGTATGCGCA CAAGCTGGAA AACTGGCAGA TGTTGCGTGG CCAAAACCTC 
ACACAAAATA AGTCCAGCTA TCCCGCCATC GAGGTGGACT ATTCTATCGA GGAGGTCAAG 
GCCAAGTACT TCAGCATGTG CGCCTCGAAA AAAAGCGCAC ACCCCTCGGT GCACGAGGAG 
GACACGGTGA ACGAAAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pat1 ( NM_001272356.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259285.1 Drosophila melanogaster protein interacting with APP tail-1, transcript variant B (Pat1), mRNA.

Price & Availability↑

CloneID	OFa00953
Clone ID Related Accession (Same CDS sequence)	NM_001272356.2
Accession Version	NM_001272356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein interacting with APP tail-1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001272356.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGGCC AACAGCTCGT GGTCTTGATC CCCAAAATAA ATCCCAACTC CCCGAAGACC 
CTATACGACC TGTCGCTGCA GGCCCTCATC GGGAGTATGA ACACCAAGAT ACCGGCGCTG 
GACAGGATCA GTCGACTGGC TGAGCTGCCA CGCAACTTGC TCATCGATGT TTACGAAATG 
ATGTCACAGC AGGAGTCCTT GAAGGACACG CTGCTGGAGG AGCTGGCCCA GCTTGAGGTA 
TTCGCTCGAC TCGTTCGCTA TCCATTTGCT CGCAGTCAAT TGCTGCGCAT AATGGCCTCA 
CTAATGGCCA GCCAAAAGAT GCTGGCCAAA AGACTCAGCG AGAACTATGT GTCGCGCTAT 
GAGGTGGTGG CCGGTCACGA AGAAGAGGAA CTGGAGTCGA GTATGGATAC AGTTGAGGAT 
CGCTCAACGG AGTTCAGTTC ACTGTATACC TACGATTCCG AATCTACAAA TGCAGACAGC 
ATCAGTGATT TTAATGACGA ATTCAGTGCT GATATCGGAA GCATAGTAGG TCTCCCACAC 
CGGAGAGCAA CACAGGAGGA ACTCGATGCG ATCAATGATT CTGGCCTCGA GGAGCTGTCC 
GCCGTTGAGC TGCAGGTCAT AGATCTGGGC CTGCGCCTCG GATCGTTCCT CTCGGAGGCT 
GGATGGATGC AGGAGAGCAT TACCGTGCTG GCATGCCTTA ATGTAAGACT GAAGGATCTG 
CCGACCCACA AACACTGGTT GCAGTTTCGA CTCGACTGCC TGCAGCGCCT TTTGTATGCC 
GAATCGGCCC ATTGCAACTT CAAGGAGGCA GAGAAAACCT ACGCGGAATT GATGGGTCTG 
AACAAATGGC TCAATAAGAG CGTGCCAAAC CAGCTGGTGG CCATAACCTA CAGTCAGATT 
TCGGCCATGT ACTTTGCCCG CAATGAGTAC AAGAACAGTC ATTTGTGGAG CGGTCTCGCC 
ATGCGATTTT TAAAAGGATA TGCGAATCCA CGCACCATCA TCGATGTTCT GCGCCAGGCG 
GCGAAGGCCT GTGTCGTTAA GCGGGACTTT GCCCGGGCCA ATCTGCTCAT CTGCCAGGCG 
GTGCGACGCG CACGCGAGTA CTTTGGCCCA ACGCACCAGA AATACGGCGA TGCACTGCTG 
GACTACGGAT TTTTCCTACT CAACGTGGAC TCGGTGTTCC AGTCTGTAAA TATATACAAA 
GAAGCACTGG CCGTGCGTCG TGGTATTTTT GGGAATATGA ACTTTCACGT GGCCATTGCC 
CATGAGGATT TGTCGTATGC CTACTACGTT CACGAGTACA GCACCGGTGA CTTTAGCTGT 
GCCCAGGATC ACGTGGACAA GGCTGTGAAT ATAATGCAGC ATCTGGTGCC AAGCAATCAT 
CTAATGTTGG CATCGGCGAA GCGCGTAAAG GCACTCCTGC TGGAGGAGAT TGCGCTGGAC 
AAGATGGCTG ATGGCATAGA TGAGGAGGAT CTGCTGCTTC AATCCGAGGA GCTGCACAAT 
TTCGCCCTGC TTCTCTCGCT GCAAGTGTTT GGCGAGGTGA ATGTACAGAC GGCGAAGCAC 
TATGGAAATC TGGGGCGGTT ATACCAAACG ATGAATCGTT TCGAAGAGGC GGAACGAATG 
CACAAAAAGG CGATCAAAAT TAAGTCGGAA CTGCTGGGTC ACTTTGACTA CGAGGTTGGC 
CTGTCCATTG GGCATTTGGC CTCGCTGTAC AATTACCAGA TGAAGAAATA CCGTGATGCC 
GAACAGCTTT ACATGCGCAG CATTGATATA AGCCTGCGTC TGTTTGGGAA CTCATACTCT 
GGCCTGGAGT ACGATTATTT GGGTCTGTGC CACGTCTACG AAACATTGCA TAACTTTGAG 
AAGTATTTGA AGTATGCGCA CAAGCTGGAA AACTGGCAGA TGTTGCGTGG CCAAAACCTC 
ACACAAAATT CCAGCTATCC CGCCATCGAG GTGGACTATT CTATCGAGGA GGTCAAGGCC 
AAGTACTTCA GCATGTGCGC CTCGAAAAAA AGCGCACACC CCTCGGTGCA CGAGGAGGAC 
ACGGTGAACG AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259285.1
CDS	369..2426
Translation	MAGQQLVVLIPKINPNSPKTLYDLSLQALIGSMNTKIPALDRISRLAELPRNLLIDVYEMMSQQESLKDTLLEELAQLEVFARLVRYPFARSQLLRIMASLMASQKMLAKRLSENYVSRYEVVAGHEEEELESSMDTVEDRSTEFSSLYTYDSESTNADSISDFNDEFSADIGSIVGLPHRRATQEELDAINDSGLEELSAVELQVIDLGLRLGSFLSEAGWMQESITVLACLNVRLKDLPTHKHWLQFRLDCLQRLLYAESAHCNFKEAEKTYAELMGLNKWLNKSVPNQLVAITYSQISAMYFARNEYKNSHLWSGLAMRFLKGYANPRTIIDVLRQAAKACVVKRDFARANLLICQAVRRAREYFGPTHQKYGDALLDYGFFLLNVDSVFQSVNIYKEALAVRRGIFGNMNFHVAIAHEDLSYAYYVHEYSTGDFSCAQDHVDKAVNIMQHLVPSNHLMLASAKRVKALLLEEIALDKMADGIDEEDLLLQSEELHNFALLLSLQVFGEVNVQTAKHYGNLGRLYQTMNRFEEAERMHKKAIKIKSELLGHFDYEVGLSIGHLASLYNYQMKKYRDAEQLYMRSIDISLRLFGNSYSGLEYDYLGLCHVYETLHNFEKYLKYAHKLENWQMLRGQNLTQNSSYPAIEVDYSIEEVKAKYFSMCASKKSAHPSVHEEDTVNEN

Target ORF information:

RefSeq Version	NM_001272356.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein interacting with APP tail-1, transcript variant B (Pat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272356.2

ORF Insert Sequence:

ATGGCTGGCC AACAGCTCGT GGTCTTGATC CCCAAAATAA ATCCCAACTC CCCGAAGACC 
CTATACGACC TGTCGCTGCA GGCCCTCATC GGGAGTATGA ACACCAAGAT ACCGGCGCTG 
GACAGGATCA GTCGACTGGC TGAGCTGCCA CGCAACTTGC TCATCGATGT TTACGAAATG 
ATGTCACAGC AGGAGTCCTT GAAGGACACG CTGCTGGAGG AGCTGGCCCA GCTTGAGGTA 
TTCGCTCGAC TCGTTCGCTA TCCATTTGCT CGCAGTCAAT TGCTGCGCAT AATGGCCTCA 
CTAATGGCCA GCCAAAAGAT GCTGGCCAAA AGACTCAGCG AGAACTATGT GTCGCGCTAT 
GAGGTGGTGG CCGGTCACGA AGAAGAGGAA CTGGAGTCGA GTATGGATAC AGTTGAGGAT 
CGCTCAACGG AGTTCAGTTC ACTGTATACC TACGATTCCG AATCTACAAA TGCAGACAGC 
ATCAGTGATT TTAATGACGA ATTCAGTGCT GATATCGGAA GCATAGTAGG TCTCCCACAC 
CGGAGAGCAA CACAGGAGGA ACTCGATGCG ATCAATGATT CTGGCCTCGA GGAGCTGTCC 
GCCGTTGAGC TGCAGGTCAT AGATCTGGGC CTGCGCCTCG GATCGTTCCT CTCGGAGGCT 
GGATGGATGC AGGAGAGCAT TACCGTGCTG GCATGCCTTA ATGTAAGACT GAAGGATCTG 
CCGACCCACA AACACTGGTT GCAGTTTCGA CTCGACTGCC TGCAGCGCCT TTTGTATGCC 
GAATCGGCCC ATTGCAACTT CAAGGAGGCA GAGAAAACCT ACGCGGAATT GATGGGTCTG 
AACAAATGGC TCAATAAGAG CGTGCCAAAC CAGCTGGTGG CCATAACCTA CAGTCAGATT 
TCGGCCATGT ACTTTGCCCG CAATGAGTAC AAGAACAGTC ATTTGTGGAG CGGTCTCGCC 
ATGCGATTTT TAAAAGGATA TGCGAATCCA CGCACCATCA TCGATGTTCT GCGCCAGGCG 
GCGAAGGCCT GTGTCGTTAA GCGGGACTTT GCCCGGGCCA ATCTGCTCAT CTGCCAGGCG 
GTGCGACGCG CACGCGAGTA CTTTGGCCCA ACGCACCAGA AATACGGCGA TGCACTGCTG 
GACTACGGAT TTTTCCTACT CAACGTGGAC TCGGTGTTCC AGTCTGTAAA TATATACAAA 
GAAGCACTGG CCGTGCGTCG TGGTATTTTT GGGAATATGA ACTTTCACGT GGCCATTGCC 
CATGAGGATT TGTCGTATGC CTACTACGTT CACGAGTACA GCACCGGTGA CTTTAGCTGT 
GCCCAGGATC ACGTGGACAA GGCTGTGAAT ATAATGCAGC ATCTGGTGCC AAGCAATCAT 
CTAATGTTGG CATCGGCGAA GCGCGTAAAG GCACTCCTGC TGGAGGAGAT TGCGCTGGAC 
AAGATGGCTG ATGGCATAGA TGAGGAGGAT CTGCTGCTTC AATCCGAGGA GCTGCACAAT 
TTCGCCCTGC TTCTCTCGCT GCAAGTGTTT GGCGAGGTGA ATGTACAGAC GGCGAAGCAC 
TATGGAAATC TGGGGCGGTT ATACCAAACG ATGAATCGTT TCGAAGAGGC GGAACGAATG 
CACAAAAAGG CGATCAAAAT TAAGTCGGAA CTGCTGGGTC ACTTTGACTA CGAGGTTGGC 
CTGTCCATTG GGCATTTGGC CTCGCTGTAC AATTACCAGA TGAAGAAATA CCGTGATGCC 
GAACAGCTTT ACATGCGCAG CATTGATATA AGCCTGCGTC TGTTTGGGAA CTCATACTCT 
GGCCTGGAGT ACGATTATTT GGGTCTGTGC CACGTCTACG AAACATTGCA TAACTTTGAG 
AAGTATTTGA AGTATGCGCA CAAGCTGGAA AACTGGCAGA TGTTGCGTGG CCAAAACCTC 
ACACAAAATT CCAGCTATCC CGCCATCGAG GTGGACTATT CTATCGAGGA GGTCAAGGCC 
AAGTACTTCA GCATGTGCGC CTCGAAAAAA AGCGCACACC CCTCGGTGCA CGAGGAGGAC 
ACGGTGAACG AAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pat1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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