Marf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Marf
Entrez Gene ID	31581
Full Name	Mitochondrial assembly regulatory factor
Synonyms	CG3869,CG38869,Dmel\CG3869,MARF,Marf-1,Mfn,Mfn-2,anon-WO0125274.3,dMFN,dMfn,dMfn2,dmfn,marf,mfn,mfn2
General protein information	Preferred Names Mitochondrial assembly regulatory factor Names CG3869 gene product from transcript CG3869-RD CG3869-PB CG3869-PC CG3869-PD Marf-PB Marf-PC Marf-PD drosophila mitofusin mitochondria assembly regulatory factor mitochondrial assembly mitochondrial assembly regulatory factor mitofusin mitofusin/MARF regulatory factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5F4-5F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_206635.3	NP_996358.2	mitochondrial assembly regulatory factor, isoform B
NM_206634.2	NP_996357.1	mitochondrial assembly regulatory factor, isoform C
NM_132092.3	NP_572320.2	mitochondrial assembly regulatory factor, isoform D

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MFN2	XP_003307856.1
Canis lupus familiaris (dog)	MFN2	XP_005618048.1
Bos taurus (cattle)	MFN2	NP_001177198.1
Mus musculus (house mouse)	Mfn2	NP_001272849.1
Rattus norvegicus (Norway rat)	Mfn2	NP_570964.4
Danio rerio (zebrafish)	mfn2	NP_001121726.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001802	XP_321258.5
Caenorhabditis elegans (roundworm)	fzo-1	NP_495161.1
Rattus norvegicus (Norway rat)	LOC100911485	XP_003750134.1
Xenopus tropicalis (tropical clawed frog)	mfn2	NP_001016514.1
	MFN2	XP_002802232.1
Gallus gallus (chicken)	MFN2	XP_004947560.1
Homo sapiens (human)	MFN2	NP_001121132.1
Drosophila melanogaster (fruit fly)	Marf	NP_996357.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Enhancing the profusion gene mitofusin/marf is beneficial in an in vivo model of TDP-43 proteinopathies, serving as a potential therapeutic target.
Title: Enhancing Mitofusin/Marf ameliorates neuromuscular dysfunction in Drosophila models of TDP-43 proteinopathies.

lack of ChChd3 leads to inactivation of Hippo activity under normal development, which is also dependent on the transcriptional coactivator Yorkie (Yki). Furthermore, loss of ChChd3 induces oxidative stress and activates the JNK pathway. In addition, depletion of other mitochondrial fusion components, Opa1 or Marf, inactivates the Hippo pathway as well.
Title: Cross-Talk Between Mitochondrial Fusion and the Hippo Pathway in Controlling Cell Proliferation During Drosophila Development.

activation of endoplasmic reticulum stress by defective mitochondria is neurotoxic in pink1 and parkin flies and that the reduction of this signalling is neuroprotective, independently of defective mitochondria.
Title: Mitofusin-mediated ER stress triggers neurodegeneration in pink1/parkin models of Parkinson's disease.

Clu is upstream of and binds to VCP in vivo and promotes VCP-dependent Marf degradation in vitro Marf accumulates in whole muscle lysates of clu-deficient flies and is destabilized upon Clu overexpression. Thus, Clu is essential for mitochondrial homeostasis and functions in concert with Parkin and VCP for Marf degradation to promote damaged mitochondrial clearance.
Title: Drosophila clueless is involved in Parkin-dependent mitophagy by promoting VCP-mediated Marf degradation.

Marf is required for mitochondrial fusion and transport in long axons.
Title: Mitochondrial fusion but not fission regulates larval growth and synaptic development through steroid hormone production.

The following Marf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Marf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03139	NM_206634.2 Latest version!	Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant C (Marf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03139	NM_206635.3 Latest version!	Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant B (Marf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa21382	NM_132092.3 Latest version!	Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant D (Marf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Marf ( NM_206635.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996358.2 Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant B (Marf), mRNA.

Price & Availability↑

CloneID	OFa03139
Clone ID Related Accession (Same CDS sequence)	NM_206635.3 , NM_206634.2
Accession Version	NM_206635.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial assembly regulatory factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206635.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT ACTTGAACCG CACCATCTCG ATGGTGACCG GGCAAACGGG CCCCGCCGAC 
GACGACCGTC ACGCCTCCTC CACGGACACG GTGGACAAAT CCGGACCCGG TTCCCCGCTA 
TCCCGGTTCA ACTCATCGCT GCAACAATCC GGCTCCACAA TGGCCGCCAA TCTGCTACCG 
GAATCGCGGC TCTATCAATC CAACGACAAA TCACCGCTCC AGATCTTTGT GCGCGCCAAA 
AAGAAGATCA ACGATATCTA CGGCGAGATC GAGGAGTATG TCCATGAGAC GACCACCTTT 
ATCAACGCCC TGCACGCGGA AGCGGAGATC GTGGACAAGG CGGAACGGGA GCTGTTCGAA 
AGCTATGTGT ACAAGGTGGC GGCCATTCGC GAAGTACTGC AGCGGGATCA CATGAAGGTG 
GCCTTCTTTG GACGCACCTC CAACGGCAAG AGCTCGGTGA TTAATGCCAT GTTGCGCGAG 
AAGATCCTGC CCAGCGGCAT TGGGCACACC ACGAATTGCT TCTGCCAAGT GGAGGGCAGC 
AATGGCGGCG AGGCGTATCT TATGACAGAG GGCTCCGAGG AGAAGCTAAA TGTGGTGAAC 
ATCAAACAAC TGGCGAATGC CTTGTGCCAG GAGAAGCTCT GCGAGAGCAG TTTGGTGCGC 
ATCTTTTGGC CGAGGGAACG CTGCAGCCTG CTGCGCGACG ATGTCGTCTT TGTGGACTCA 
CCTGGTGTGG ATGTGTCGGC CAATTTGGAC GACTGGATCG ATAACCATTG CCTGAACGCC 
GATGTCTTTG TGCTGGTCCT GAATGCCGAG TCAACGATGA CGCGTGCGGA GAAGCAGTTC 
TTTCACACCG TCTCGCAGAA GCTAAGCAAG CCGAACATCT TCATCCTGAA CAATCGCTGG 
GATGCGTCGG CCAACGAGCC CGAGTGCCAG GAATCGGTTA AGTCTCAGCA CACGGAACGC 
TGCATCGACT TCCTCACCAA GGAGCTAAAG GTGAGCAACG AGAAGGAGGC GGCCGAAAGG 
GTATTCTTCG TTTCCGCCAG GGAAACGCTG CAGGCGCGCA TCGAGGAGGC CAAGGGCAAT 
CCGCCGCACA TGGGTGCCAT TGCCGAGGGC TTTCAGATAC GCTACTTCGA GTTCCAGGAC 
TTCGAGCGCA AGTTCGAGGA GTGCATCTCG CAGAGTGCTG TGAAAACAAA GTTCCAGCAG 
CACAGTTCGC GCGGCAAGAG TGTTTCGGGT GACATGAAAT CAATGTTGGA CAACATTTAC 
GAGCGGATCA CCATATTCCG CAATCTGAAG CAGGACCAGA AGAACCTGCT CACCGAACGC 
ATCCAGGGCA CAGAGACGCA GATGATGCAG GTTACGCGGG AAATGAAGAT GAAGATCCAC 
AACATGGTCG AGGAGGTGGA GGAGAAGGTG TCAAAGGCGC TGAACGAGGA GATCTGGCGC 
CTGGGCGTAC TCATTGACGA GTTCAATATG CCCTTCCATC CGGAGCGTTT GGTGCTGAAC 
ATCTACAAAA AGGAATTGAA TGCCCATGTG GAGAGCGGCC TGGGCAGCAA TCTGCGCGCC 
CGCCTATCCA TGGCCCTGGC CATGAACGTC GAGTCCGCCC AGACGGAGAT GACCGATCGC 
ATGCACGCCC TGGTGCCCAA CGAGCAGCTC CTGGCCACCA GCACCAAGAT GGTGGTGCGC 
ACGCAGCCCT TCGAGATGCT CTACTCACTG AATTGCCAGA ACCTCTGCGC CGACTTCCAG 
GAGGATCTGG AGTTTAAGTT CAGCTGGGGC ATTGCGGCGA TGATCCAGCG CTTCACCGGC 
AAGGTGCGCG AGCGCAGCAA GAAGGGCCAG CCGGCGCTGG TCAATCGACA GAGCAGTATT 
GGACACAGCG TATCGACGCC GACCACGACG CCCGTGGAAG CCACTCCTGT GTGCCTGCTG 
CCCGCTCCCG TTGTGGCTGG CATTACGCCC GAGCAGCTGT CGTTGATCTC ACGCTTCGCG 
GTGTCCTCCA TTGGATCGCA GGGCACCGTT GGTGGTCTCG TCGTAGCCGG TGTCATGCTG 
AAAACTATCG GCTGGCGTGT CCTGGTGGGC GTGGGTGCCC TCTACGGCTG CATCTACCTG 
TACGAGCGCC TCTCGTGGAC AAATTCAGCC AAGGAGCGAA CGTTCAAGTC GCAGTACGTG 
CGCCATGCCA CCAAGAAGCT CAAGATGATT GTCGACCTCA CCTCGGCCAA CTGCAGCCAC 
CAAGTGCAGC AGGAATTGTC GAGCACCTTT GCCCGTTTGT GCCGCACGGT TGATACCGCC 
ACCACGGACA TGAATGATGA GCTCAAGACG CTGGACTCGC AGCTGAACAT CCTGGAGGCG 
AACCAGAAGC AATTGAAGCT GCTTCGCAAC AAGGCCAACT ACATACAGAA CGAGCTGGAC 
ATCTTCGAGC ACAACTATAT ATCGCCGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996358.2
CDS	348..2780
Translation	MAAYLNRTISMVTGQTGPADDDRHASSTDTVDKSGPGSPLSRFNSSLQQSGSTMAANLLPESRLYQSNDKSPLQIFVRAKKKINDIYGEIEEYVHETTTFINALHAEAEIVDKAERELFESYVYKVAAIREVLQRDHMKVAFFGRTSNGKSSVINAMLREKILPSGIGHTTNCFCQVEGSNGGEAYLMTEGSEEKLNVVNIKQLANALCQEKLCESSLVRIFWPRERCSLLRDDVVFVDSPGVDVSANLDDWIDNHCLNADVFVLVLNAESTMTRAEKQFFHTVSQKLSKPNIFILNNRWDASANEPECQESVKSQHTERCIDFLTKELKVSNEKEAAERVFFVSARETLQARIEEAKGNPPHMGAIAEGFQIRYFEFQDFERKFEECISQSAVKTKFQQHSSRGKSVSGDMKSMLDNIYERITIFRNLKQDQKNLLTERIQGTETQMMQVTREMKMKIHNMVEEVEEKVSKALNEEIWRLGVLIDEFNMPFHPERLVLNIYKKELNAHVESGLGSNLRARLSMALAMNVESAQTEMTDRMHALVPNEQLLATSTKMVVRTQPFEMLYSLNCQNLCADFQEDLEFKFSWGIAAMIQRFTGKVRERSKKGQPALVNRQSSIGHSVSTPTTTPVEATPVCLLPAPVVAGITPEQLSLISRFAVSSIGSQGTVGGLVVAGVMLKTIGWRVLVGVGALYGCIYLYERLSWTNSAKERTFKSQYVRHATKKLKMIVDLTSANCSHQVQQELSSTFARLCRTVDTATTDMNDELKTLDSQLNILEANQKQLKLLRNKANYIQNELDIFEHNYISPQ

Target ORF information:

RefSeq Version	NM_206635.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant B (Marf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206635.3

ORF Insert Sequence:

ATGGCGGCCT ACTTGAACCG CACCATCTCG ATGGTGACCG GGCAAACGGG CCCCGCCGAC 
GACGACCGTC ACGCCTCCTC CACGGACACG GTGGACAAAT CCGGACCCGG TTCCCCGCTA 
TCCCGGTTCA ACTCATCGCT GCAACAATCC GGCTCCACAA TGGCCGCCAA TCTGCTACCG 
GAATCGCGGC TCTATCAATC CAACGACAAA TCACCGCTCC AGATCTTTGT GCGCGCCAAA 
AAGAAGATCA ACGATATCTA CGGCGAGATC GAGGAGTATG TCCATGAGAC GACCACCTTT 
ATCAACGCCC TGCACGCGGA AGCGGAGATC GTGGACAAGG CGGAACGGGA GCTGTTCGAA 
AGCTATGTGT ACAAGGTGGC GGCCATTCGC GAAGTACTGC AGCGGGATCA CATGAAGGTG 
GCCTTCTTTG GACGCACCTC CAACGGCAAG AGCTCGGTGA TTAATGCCAT GTTGCGCGAG 
AAGATCCTGC CCAGCGGCAT TGGGCACACC ACGAATTGCT TCTGCCAAGT GGAGGGCAGC 
AATGGCGGCG AGGCGTATCT TATGACAGAG GGCTCCGAGG AGAAGCTAAA TGTGGTGAAC 
ATCAAACAAC TGGCGAATGC CTTGTGCCAG GAGAAGCTCT GCGAGAGCAG TTTGGTGCGC 
ATCTTTTGGC CGAGGGAACG CTGCAGCCTG CTGCGCGACG ATGTCGTCTT TGTGGACTCA 
CCTGGTGTGG ATGTGTCGGC CAATTTGGAC GACTGGATCG ATAACCATTG CCTGAACGCC 
GATGTCTTTG TGCTGGTCCT GAATGCCGAG TCAACGATGA CGCGTGCGGA GAAGCAGTTC 
TTTCACACCG TCTCGCAGAA GCTAAGCAAG CCGAACATCT TCATCCTGAA CAATCGCTGG 
GATGCGTCGG CCAACGAGCC CGAGTGCCAG GAATCGGTTA AGTCTCAGCA CACGGAACGC 
TGCATCGACT TCCTCACCAA GGAGCTAAAG GTGAGCAACG AGAAGGAGGC GGCCGAAAGG 
GTATTCTTCG TTTCCGCCAG GGAAACGCTG CAGGCGCGCA TCGAGGAGGC CAAGGGCAAT 
CCGCCGCACA TGGGTGCCAT TGCCGAGGGC TTTCAGATAC GCTACTTCGA GTTCCAGGAC 
TTCGAGCGCA AGTTCGAGGA GTGCATCTCG CAGAGTGCTG TGAAAACAAA GTTCCAGCAG 
CACAGTTCGC GCGGCAAGAG TGTTTCGGGT GACATGAAAT CAATGTTGGA CAACATTTAC 
GAGCGGATCA CCATATTCCG CAATCTGAAG CAGGACCAGA AGAACCTGCT CACCGAACGC 
ATCCAGGGCA CAGAGACGCA GATGATGCAG GTTACGCGGG AAATGAAGAT GAAGATCCAC 
AACATGGTCG AGGAGGTGGA GGAGAAGGTG TCAAAGGCGC TGAACGAGGA GATCTGGCGC 
CTGGGCGTAC TCATTGACGA GTTCAATATG CCCTTCCATC CGGAGCGTTT GGTGCTGAAC 
ATCTACAAAA AGGAATTGAA TGCCCATGTG GAGAGCGGCC TGGGCAGCAA TCTGCGCGCC 
CGCCTATCCA TGGCCCTGGC CATGAACGTC GAGTCCGCCC AGACGGAGAT GACCGATCGC 
ATGCACGCCC TGGTGCCCAA CGAGCAGCTC CTGGCCACCA GCACCAAGAT GGTGGTGCGC 
ACGCAGCCCT TCGAGATGCT CTACTCACTG AATTGCCAGA ACCTCTGCGC CGACTTCCAG 
GAGGATCTGG AGTTTAAGTT CAGCTGGGGC ATTGCGGCGA TGATCCAGCG CTTCACCGGC 
AAGGTGCGCG AGCGCAGCAA GAAGGGCCAG CCGGCGCTGG TCAATCGACA GAGCAGTATT 
GGACACAGCG TATCGACGCC GACCACGACG CCCGTGGAAG CCACTCCTGT GTGCCTGCTG 
CCCGCTCCCG TTGTGGCTGG CATTACGCCC GAGCAGCTGT CGTTGATCTC ACGCTTCGCG 
GTGTCCTCCA TTGGATCGCA GGGCACCGTT GGTGGTCTCG TCGTAGCCGG TGTCATGCTG 
AAAACTATCG GCTGGCGTGT CCTGGTGGGC GTGGGTGCCC TCTACGGCTG CATCTACCTG 
TACGAGCGCC TCTCGTGGAC AAATTCAGCC AAGGAGCGAA CGTTCAAGTC GCAGTACGTG 
CGCCATGCCA CCAAGAAGCT CAAGATGATT GTCGACCTCA CCTCGGCCAA CTGCAGCCAC 
CAAGTGCAGC AGGAATTGTC GAGCACCTTT GCCCGTTTGT GCCGCACGGT TGATACCGCC 
ACCACGGACA TGAATGATGA GCTCAAGACG CTGGACTCGC AGCTGAACAT CCTGGAGGCG 
AACCAGAAGC AATTGAAGCT GCTTCGCAAC AAGGCCAACT ACATACAGAA CGAGCTGGAC 
ATCTTCGAGC ACAACTATAT ATCGCCGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Marf ( NM_206634.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996357.1 Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant C (Marf), mRNA.

Price & Availability↑

CloneID	OFa03139
Clone ID Related Accession (Same CDS sequence)	NM_206635.3 , NM_206634.2
Accession Version	NM_206634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial assembly regulatory factor, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206634.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT ACTTGAACCG CACCATCTCG ATGGTGACCG GGCAAACGGG CCCCGCCGAC 
GACGACCGTC ACGCCTCCTC CACGGACACG GTGGACAAAT CCGGACCCGG TTCCCCGCTA 
TCCCGGTTCA ACTCATCGCT GCAACAATCC GGCTCCACAA TGGCCGCCAA TCTGCTACCG 
GAATCGCGGC TCTATCAATC CAACGACAAA TCACCGCTCC AGATCTTTGT GCGCGCCAAA 
AAGAAGATCA ACGATATCTA CGGCGAGATC GAGGAGTATG TCCATGAGAC GACCACCTTT 
ATCAACGCCC TGCACGCGGA AGCGGAGATC GTGGACAAGG CGGAACGGGA GCTGTTCGAA 
AGCTATGTGT ACAAGGTGGC GGCCATTCGC GAAGTACTGC AGCGGGATCA CATGAAGGTG 
GCCTTCTTTG GACGCACCTC CAACGGCAAG AGCTCGGTGA TTAATGCCAT GTTGCGCGAG 
AAGATCCTGC CCAGCGGCAT TGGGCACACC ACGAATTGCT TCTGCCAAGT GGAGGGCAGC 
AATGGCGGCG AGGCGTATCT TATGACAGAG GGCTCCGAGG AGAAGCTAAA TGTGGTGAAC 
ATCAAACAAC TGGCGAATGC CTTGTGCCAG GAGAAGCTCT GCGAGAGCAG TTTGGTGCGC 
ATCTTTTGGC CGAGGGAACG CTGCAGCCTG CTGCGCGACG ATGTCGTCTT TGTGGACTCA 
CCTGGTGTGG ATGTGTCGGC CAATTTGGAC GACTGGATCG ATAACCATTG CCTGAACGCC 
GATGTCTTTG TGCTGGTCCT GAATGCCGAG TCAACGATGA CGCGTGCGGA GAAGCAGTTC 
TTTCACACCG TCTCGCAGAA GCTAAGCAAG CCGAACATCT TCATCCTGAA CAATCGCTGG 
GATGCGTCGG CCAACGAGCC CGAGTGCCAG GAATCGGTTA AGTCTCAGCA CACGGAACGC 
TGCATCGACT TCCTCACCAA GGAGCTAAAG GTGAGCAACG AGAAGGAGGC GGCCGAAAGG 
GTATTCTTCG TTTCCGCCAG GGAAACGCTG CAGGCGCGCA TCGAGGAGGC CAAGGGCAAT 
CCGCCGCACA TGGGTGCCAT TGCCGAGGGC TTTCAGATAC GCTACTTCGA GTTCCAGGAC 
TTCGAGCGCA AGTTCGAGGA GTGCATCTCG CAGAGTGCTG TGAAAACAAA GTTCCAGCAG 
CACAGTTCGC GCGGCAAGAG TGTTTCGGGT GACATGAAAT CAATGTTGGA CAACATTTAC 
GAGCGGATCA CCATATTCCG CAATCTGAAG CAGGACCAGA AGAACCTGCT CACCGAACGC 
ATCCAGGGCA CAGAGACGCA GATGATGCAG GTTACGCGGG AAATGAAGAT GAAGATCCAC 
AACATGGTCG AGGAGGTGGA GGAGAAGGTG TCAAAGGCGC TGAACGAGGA GATCTGGCGC 
CTGGGCGTAC TCATTGACGA GTTCAATATG CCCTTCCATC CGGAGCGTTT GGTGCTGAAC 
ATCTACAAAA AGGAATTGAA TGCCCATGTG GAGAGCGGCC TGGGCAGCAA TCTGCGCGCC 
CGCCTATCCA TGGCCCTGGC CATGAACGTC GAGTCCGCCC AGACGGAGAT GACCGATCGC 
ATGCACGCCC TGGTGCCCAA CGAGCAGCTC CTGGCCACCA GCACCAAGAT GGTGGTGCGC 
ACGCAGCCCT TCGAGATGCT CTACTCACTG AATTGCCAGA ACCTCTGCGC CGACTTCCAG 
GAGGATCTGG AGTTTAAGTT CAGCTGGGGC ATTGCGGCGA TGATCCAGCG CTTCACCGGC 
AAGGTGCGCG AGCGCAGCAA GAAGGGCCAG CCGGCGCTGG TCAATCGACA GAGCAGTATT 
GGACACAGCG TATCGACGCC GACCACGACG CCCGTGGAAG CCACTCCTGT GTGCCTGCTG 
CCCGCTCCCG TTGTGGCTGG CATTACGCCC GAGCAGCTGT CGTTGATCTC ACGCTTCGCG 
GTGTCCTCCA TTGGATCGCA GGGCACCGTT GGTGGTCTCG TCGTAGCCGG TGTCATGCTG 
AAAACTATCG GCTGGCGTGT CCTGGTGGGC GTGGGTGCCC TCTACGGCTG CATCTACCTG 
TACGAGCGCC TCTCGTGGAC AAATTCAGCC AAGGAGCGAA CGTTCAAGTC GCAGTACGTG 
CGCCATGCCA CCAAGAAGCT CAAGATGATT GTCGACCTCA CCTCGGCCAA CTGCAGCCAC 
CAAGTGCAGC AGGAATTGTC GAGCACCTTT GCCCGTTTGT GCCGCACGGT TGATACCGCC 
ACCACGGACA TGAATGATGA GCTCAAGACG CTGGACTCGC AGCTGAACAT CCTGGAGGCG 
AACCAGAAGC AATTGAAGCT GCTTCGCAAC AAGGCCAACT ACATACAGAA CGAGCTGGAC 
ATCTTCGAGC ACAACTATAT ATCGCCGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996357.1
CDS	509..2941
Translation	MAAYLNRTISMVTGQTGPADDDRHASSTDTVDKSGPGSPLSRFNSSLQQSGSTMAANLLPESRLYQSNDKSPLQIFVRAKKKINDIYGEIEEYVHETTTFINALHAEAEIVDKAERELFESYVYKVAAIREVLQRDHMKVAFFGRTSNGKSSVINAMLREKILPSGIGHTTNCFCQVEGSNGGEAYLMTEGSEEKLNVVNIKQLANALCQEKLCESSLVRIFWPRERCSLLRDDVVFVDSPGVDVSANLDDWIDNHCLNADVFVLVLNAESTMTRAEKQFFHTVSQKLSKPNIFILNNRWDASANEPECQESVKSQHTERCIDFLTKELKVSNEKEAAERVFFVSARETLQARIEEAKGNPPHMGAIAEGFQIRYFEFQDFERKFEECISQSAVKTKFQQHSSRGKSVSGDMKSMLDNIYERITIFRNLKQDQKNLLTERIQGTETQMMQVTREMKMKIHNMVEEVEEKVSKALNEEIWRLGVLIDEFNMPFHPERLVLNIYKKELNAHVESGLGSNLRARLSMALAMNVESAQTEMTDRMHALVPNEQLLATSTKMVVRTQPFEMLYSLNCQNLCADFQEDLEFKFSWGIAAMIQRFTGKVRERSKKGQPALVNRQSSIGHSVSTPTTTPVEATPVCLLPAPVVAGITPEQLSLISRFAVSSIGSQGTVGGLVVAGVMLKTIGWRVLVGVGALYGCIYLYERLSWTNSAKERTFKSQYVRHATKKLKMIVDLTSANCSHQVQQELSSTFARLCRTVDTATTDMNDELKTLDSQLNILEANQKQLKLLRNKANYIQNELDIFEHNYISPQ

Target ORF information:

RefSeq Version	NM_206634.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant C (Marf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206634.2

ORF Insert Sequence:

ATGGCGGCCT ACTTGAACCG CACCATCTCG ATGGTGACCG GGCAAACGGG CCCCGCCGAC 
GACGACCGTC ACGCCTCCTC CACGGACACG GTGGACAAAT CCGGACCCGG TTCCCCGCTA 
TCCCGGTTCA ACTCATCGCT GCAACAATCC GGCTCCACAA TGGCCGCCAA TCTGCTACCG 
GAATCGCGGC TCTATCAATC CAACGACAAA TCACCGCTCC AGATCTTTGT GCGCGCCAAA 
AAGAAGATCA ACGATATCTA CGGCGAGATC GAGGAGTATG TCCATGAGAC GACCACCTTT 
ATCAACGCCC TGCACGCGGA AGCGGAGATC GTGGACAAGG CGGAACGGGA GCTGTTCGAA 
AGCTATGTGT ACAAGGTGGC GGCCATTCGC GAAGTACTGC AGCGGGATCA CATGAAGGTG 
GCCTTCTTTG GACGCACCTC CAACGGCAAG AGCTCGGTGA TTAATGCCAT GTTGCGCGAG 
AAGATCCTGC CCAGCGGCAT TGGGCACACC ACGAATTGCT TCTGCCAAGT GGAGGGCAGC 
AATGGCGGCG AGGCGTATCT TATGACAGAG GGCTCCGAGG AGAAGCTAAA TGTGGTGAAC 
ATCAAACAAC TGGCGAATGC CTTGTGCCAG GAGAAGCTCT GCGAGAGCAG TTTGGTGCGC 
ATCTTTTGGC CGAGGGAACG CTGCAGCCTG CTGCGCGACG ATGTCGTCTT TGTGGACTCA 
CCTGGTGTGG ATGTGTCGGC CAATTTGGAC GACTGGATCG ATAACCATTG CCTGAACGCC 
GATGTCTTTG TGCTGGTCCT GAATGCCGAG TCAACGATGA CGCGTGCGGA GAAGCAGTTC 
TTTCACACCG TCTCGCAGAA GCTAAGCAAG CCGAACATCT TCATCCTGAA CAATCGCTGG 
GATGCGTCGG CCAACGAGCC CGAGTGCCAG GAATCGGTTA AGTCTCAGCA CACGGAACGC 
TGCATCGACT TCCTCACCAA GGAGCTAAAG GTGAGCAACG AGAAGGAGGC GGCCGAAAGG 
GTATTCTTCG TTTCCGCCAG GGAAACGCTG CAGGCGCGCA TCGAGGAGGC CAAGGGCAAT 
CCGCCGCACA TGGGTGCCAT TGCCGAGGGC TTTCAGATAC GCTACTTCGA GTTCCAGGAC 
TTCGAGCGCA AGTTCGAGGA GTGCATCTCG CAGAGTGCTG TGAAAACAAA GTTCCAGCAG 
CACAGTTCGC GCGGCAAGAG TGTTTCGGGT GACATGAAAT CAATGTTGGA CAACATTTAC 
GAGCGGATCA CCATATTCCG CAATCTGAAG CAGGACCAGA AGAACCTGCT CACCGAACGC 
ATCCAGGGCA CAGAGACGCA GATGATGCAG GTTACGCGGG AAATGAAGAT GAAGATCCAC 
AACATGGTCG AGGAGGTGGA GGAGAAGGTG TCAAAGGCGC TGAACGAGGA GATCTGGCGC 
CTGGGCGTAC TCATTGACGA GTTCAATATG CCCTTCCATC CGGAGCGTTT GGTGCTGAAC 
ATCTACAAAA AGGAATTGAA TGCCCATGTG GAGAGCGGCC TGGGCAGCAA TCTGCGCGCC 
CGCCTATCCA TGGCCCTGGC CATGAACGTC GAGTCCGCCC AGACGGAGAT GACCGATCGC 
ATGCACGCCC TGGTGCCCAA CGAGCAGCTC CTGGCCACCA GCACCAAGAT GGTGGTGCGC 
ACGCAGCCCT TCGAGATGCT CTACTCACTG AATTGCCAGA ACCTCTGCGC CGACTTCCAG 
GAGGATCTGG AGTTTAAGTT CAGCTGGGGC ATTGCGGCGA TGATCCAGCG CTTCACCGGC 
AAGGTGCGCG AGCGCAGCAA GAAGGGCCAG CCGGCGCTGG TCAATCGACA GAGCAGTATT 
GGACACAGCG TATCGACGCC GACCACGACG CCCGTGGAAG CCACTCCTGT GTGCCTGCTG 
CCCGCTCCCG TTGTGGCTGG CATTACGCCC GAGCAGCTGT CGTTGATCTC ACGCTTCGCG 
GTGTCCTCCA TTGGATCGCA GGGCACCGTT GGTGGTCTCG TCGTAGCCGG TGTCATGCTG 
AAAACTATCG GCTGGCGTGT CCTGGTGGGC GTGGGTGCCC TCTACGGCTG CATCTACCTG 
TACGAGCGCC TCTCGTGGAC AAATTCAGCC AAGGAGCGAA CGTTCAAGTC GCAGTACGTG 
CGCCATGCCA CCAAGAAGCT CAAGATGATT GTCGACCTCA CCTCGGCCAA CTGCAGCCAC 
CAAGTGCAGC AGGAATTGTC GAGCACCTTT GCCCGTTTGT GCCGCACGGT TGATACCGCC 
ACCACGGACA TGAATGATGA GCTCAAGACG CTGGACTCGC AGCTGAACAT CCTGGAGGCG 
AACCAGAAGC AATTGAAGCT GCTTCGCAAC AAGGCCAACT ACATACAGAA CGAGCTGGAC 
ATCTTCGAGC ACAACTATAT ATCGCCGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Marf ( NM_132092.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572320.2 Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant D (Marf), mRNA.

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CloneID	OFa21382
Clone ID Related Accession (Same CDS sequence)	NM_132092.3
Accession Version	NM_132092.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial assembly regulatory factor, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132092.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGCCT ACTTGAACCG CACCATCTCG ATGGTGACCG GGCAAACGGG CCCCGCCGAC 
GACGACCGTC ACGCCTCCTC CACGGACACG GTGGACAAAT CCGGACCCGG TTCCCCGCTA 
TCCCGGTTCA ACTCATCGCT GCAACAATCC GGCTCCACAA TGGCCGCCAA TCTGCTACCG 
GAATCGCGGC TCTATCAATC CAACGACAAA TCACCGCTCC AGATCTTTGT GCGCGCCAAA 
AAGAAGATCA ACGATATCTA CGGCGAGATC GAGGAGTATG TCCATGAGAC GACCACCTTT 
ATCAACGCCC TGCACGCGGA AGCGGAGATC GTGGACAAGG CGGAACGGGA GCTGTTCGAA 
AGCTATGTGT ACAAGGTGGC GGCCATTCGC GAAGTACTGC AGCGGGATCA CATGAAGGTG 
GCCTTCTTTG GACGCACCTC CAACGGCAAG AGCTCGGTGA TTAATGCCAT GTTGCGCGAG 
AAGATCCTGC CCAGCGGCAT TGGGCACACC ACGAATTGCT TCTGCCAAGT GGAGGGCAGC 
AATGGCGGCG AGGCGTATCT TATGACAGAG GGCTCCGAGG AGAAGCTAAA TGTGGTGAAC 
ATCAAACAAC TGGCGAATGC CTTGTGCCAG GAGAAGCTCT GCGAGAGCAG TTTGGTGCGC 
ATCTTTTGGC CGAGGGAACG CTGCAGCCTG CTGCGCGACG ATGTCGTCTT TGTGGACTCA 
CCTGGTGTGG ATGTGTCGGC CAATTTGGAC GACTGGATCG ATAACCATTG CCTGAACGCC 
GATGTCTTTG TGCTGGTCCT GAATGCCGAG TCAACGATGA CGCGTGCGGA GAAGCAGTTC 
TTTCACACCG TCTCGCAGAA GCTAAGCAAG CCGAACATCT TCATCCTGAA CAATCGCTGG 
GATGCGTCGG CCAACGAGCC CGAGTGCCAG GAATCGGAAT TGGCTAAGGT TAAGTCTCAG 
CACACGGAAC GCTGCATCGA CTTCCTCACC AAGGAGCTAA AGGTGAGCAA CGAGAAGGAG 
GCGGCCGAAA GGGTATTCTT CGTTTCCGCC AGGGAAACGC TGCAGGCGCG CATCGAGGAG 
GCCAAGGGCA ATCCGCCGCA CATGGGTGCC ATTGCCGAGG GCTTTCAGAT ACGCTACTTC 
GAGTTCCAGG ACTTCGAGCG CAAGTTCGAG GAGTGCATCT CGCAGAGTGC TGTGAAAACA 
AAGTTCCAGC AGCACAGTTC GCGCGGCAAG AGTGTTTCGG GTGACATGAA ATCAATGTTG 
GACAACATTT ACGAGCGGAT CACCATATTC CGCAATCTGA AGCAGGACCA GAAGAACCTG 
CTCACCGAAC GCATCCAGGG CACAGAGACG CAGATGATGC AGGTTACGCG GGAAATGAAG 
ATGAAGATCC ACAACATGGT CGAGGAGGTG GAGGAGAAGG TGTCAAAGGC GCTGAACGAG 
GAGATCTGGC GCCTGGGCGT ACTCATTGAC GAGTTCAATA TGCCCTTCCA TCCGGAGCGT 
TTGGTGCTGA ACATCTACAA AAAGGAATTG AATGCCCATG TGGAGAGCGG CCTGGGCAGC 
AATCTGCGCG CCCGCCTATC CATGGCCCTG GCCATGAACG TCGAGTCCGC CCAGACGGAG 
ATGACCGATC GCATGCACGC CCTGGTGCCC AACGAGCAGC TCCTGGCCAC CAGCACCAAG 
ATGGTGGTGC GCACGCAGCC CTTCGAGATG CTCTACTCAC TGAATTGCCA GAACCTCTGC 
GCCGACTTCC AGGAGGATCT GGAGTTTAAG TTCAGCTGGG GCATTGCGGC GATGATCCAG 
CGCTTCACCG GCAAGGTGCG CGAGCGCAGC AAGAAGGGCC AGCCGGCGCT GGTCAATCGA 
CAGAGCAGTA TTGGACACAG CGTATCGACG CCGACCACGA CGCCCGTGGA AGCCACTCCT 
GTGTGCCTGC TGCCCGCTCC CGTTGTGGCT GGCATTACGC CCGAGCAGCT GTCGTTGATC 
TCACGCTTCG CGGTGTCCTC CATTGGATCG CAGGGCACCG TTGGTGGTCT CGTCGTAGCC 
GGTGTCATGC TGAAAACTAT CGGCTGGCGT GTCCTGGTGG GCGTGGGTGC CCTCTACGGC 
TGCATCTACC TGTACGAGCG CCTCTCGTGG ACAAATTCAG CCAAGGAGCG AACGTTCAAG 
TCGCAGTACG TGCGCCATGC CACCAAGAAG CTCAAGATGA TTGTCGACCT CACCTCGGCC 
AACTGCAGCC ACCAAGTGCA GCAGGAATTG TCGAGCACCT TTGCCCGTTT GTGCCGCACG 
GTTGATACCG CCACCACGGA CATGAATGAT GAGCTCAAGA CGCTGGACTC GCAGCTGAAC 
ATCCTGGAGG CGAACCAGAA GCAATTGAAG CTGCTTCGCA ACAAGGCCAA CTACATACAG 
AACGAGCTGG ACATCTTCGA GCACAACTAT ATATCGCCGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572320.2
CDS	509..2953
Translation	MAAYLNRTISMVTGQTGPADDDRHASSTDTVDKSGPGSPLSRFNSSLQQSGSTMAANLLPESRLYQSNDKSPLQIFVRAKKKINDIYGEIEEYVHETTTFINALHAEAEIVDKAERELFESYVYKVAAIREVLQRDHMKVAFFGRTSNGKSSVINAMLREKILPSGIGHTTNCFCQVEGSNGGEAYLMTEGSEEKLNVVNIKQLANALCQEKLCESSLVRIFWPRERCSLLRDDVVFVDSPGVDVSANLDDWIDNHCLNADVFVLVLNAESTMTRAEKQFFHTVSQKLSKPNIFILNNRWDASANEPECQESELAKVKSQHTERCIDFLTKELKVSNEKEAAERVFFVSARETLQARIEEAKGNPPHMGAIAEGFQIRYFEFQDFERKFEECISQSAVKTKFQQHSSRGKSVSGDMKSMLDNIYERITIFRNLKQDQKNLLTERIQGTETQMMQVTREMKMKIHNMVEEVEEKVSKALNEEIWRLGVLIDEFNMPFHPERLVLNIYKKELNAHVESGLGSNLRARLSMALAMNVESAQTEMTDRMHALVPNEQLLATSTKMVVRTQPFEMLYSLNCQNLCADFQEDLEFKFSWGIAAMIQRFTGKVRERSKKGQPALVNRQSSIGHSVSTPTTTPVEATPVCLLPAPVVAGITPEQLSLISRFAVSSIGSQGTVGGLVVAGVMLKTIGWRVLVGVGALYGCIYLYERLSWTNSAKERTFKSQYVRHATKKLKMIVDLTSANCSHQVQQELSSTFARLCRTVDTATTDMNDELKTLDSQLNILEANQKQLKLLRNKANYIQNELDIFEHNYISPQ

Target ORF information:

RefSeq Version	NM_132092.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial assembly regulatory factor, transcript variant D (Marf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132092.3

ORF Insert Sequence:

ATGGCGGCCT ACTTGAACCG CACCATCTCG ATGGTGACCG GGCAAACGGG CCCCGCCGAC 
GACGACCGTC ACGCCTCCTC CACGGACACG GTGGACAAAT CCGGACCCGG TTCCCCGCTA 
TCCCGGTTCA ACTCATCGCT GCAACAATCC GGCTCCACAA TGGCCGCCAA TCTGCTACCG 
GAATCGCGGC TCTATCAATC CAACGACAAA TCACCGCTCC AGATCTTTGT GCGCGCCAAA 
AAGAAGATCA ACGATATCTA CGGCGAGATC GAGGAGTATG TCCATGAGAC GACCACCTTT 
ATCAACGCCC TGCACGCGGA AGCGGAGATC GTGGACAAGG CGGAACGGGA GCTGTTCGAA 
AGCTATGTGT ACAAGGTGGC GGCCATTCGC GAAGTACTGC AGCGGGATCA CATGAAGGTG 
GCCTTCTTTG GACGCACCTC CAACGGCAAG AGCTCGGTGA TTAATGCCAT GTTGCGCGAG 
AAGATCCTGC CCAGCGGCAT TGGGCACACC ACGAATTGCT TCTGCCAAGT GGAGGGCAGC 
AATGGCGGCG AGGCGTATCT TATGACAGAG GGCTCCGAGG AGAAGCTAAA TGTGGTGAAC 
ATCAAACAAC TGGCGAATGC CTTGTGCCAG GAGAAGCTCT GCGAGAGCAG TTTGGTGCGC 
ATCTTTTGGC CGAGGGAACG CTGCAGCCTG CTGCGCGACG ATGTCGTCTT TGTGGACTCA 
CCTGGTGTGG ATGTGTCGGC CAATTTGGAC GACTGGATCG ATAACCATTG CCTGAACGCC 
GATGTCTTTG TGCTGGTCCT GAATGCCGAG TCAACGATGA CGCGTGCGGA GAAGCAGTTC 
TTTCACACCG TCTCGCAGAA GCTAAGCAAG CCGAACATCT TCATCCTGAA CAATCGCTGG 
GATGCGTCGG CCAACGAGCC CGAGTGCCAG GAATCGGAAT TGGCTAAGGT TAAGTCTCAG 
CACACGGAAC GCTGCATCGA CTTCCTCACC AAGGAGCTAA AGGTGAGCAA CGAGAAGGAG 
GCGGCCGAAA GGGTATTCTT CGTTTCCGCC AGGGAAACGC TGCAGGCGCG CATCGAGGAG 
GCCAAGGGCA ATCCGCCGCA CATGGGTGCC ATTGCCGAGG GCTTTCAGAT ACGCTACTTC 
GAGTTCCAGG ACTTCGAGCG CAAGTTCGAG GAGTGCATCT CGCAGAGTGC TGTGAAAACA 
AAGTTCCAGC AGCACAGTTC GCGCGGCAAG AGTGTTTCGG GTGACATGAA ATCAATGTTG 
GACAACATTT ACGAGCGGAT CACCATATTC CGCAATCTGA AGCAGGACCA GAAGAACCTG 
CTCACCGAAC GCATCCAGGG CACAGAGACG CAGATGATGC AGGTTACGCG GGAAATGAAG 
ATGAAGATCC ACAACATGGT CGAGGAGGTG GAGGAGAAGG TGTCAAAGGC GCTGAACGAG 
GAGATCTGGC GCCTGGGCGT ACTCATTGAC GAGTTCAATA TGCCCTTCCA TCCGGAGCGT 
TTGGTGCTGA ACATCTACAA AAAGGAATTG AATGCCCATG TGGAGAGCGG CCTGGGCAGC 
AATCTGCGCG CCCGCCTATC CATGGCCCTG GCCATGAACG TCGAGTCCGC CCAGACGGAG 
ATGACCGATC GCATGCACGC CCTGGTGCCC AACGAGCAGC TCCTGGCCAC CAGCACCAAG 
ATGGTGGTGC GCACGCAGCC CTTCGAGATG CTCTACTCAC TGAATTGCCA GAACCTCTGC 
GCCGACTTCC AGGAGGATCT GGAGTTTAAG TTCAGCTGGG GCATTGCGGC GATGATCCAG 
CGCTTCACCG GCAAGGTGCG CGAGCGCAGC AAGAAGGGCC AGCCGGCGCT GGTCAATCGA 
CAGAGCAGTA TTGGACACAG CGTATCGACG CCGACCACGA CGCCCGTGGA AGCCACTCCT 
GTGTGCCTGC TGCCCGCTCC CGTTGTGGCT GGCATTACGC CCGAGCAGCT GTCGTTGATC 
TCACGCTTCG CGGTGTCCTC CATTGGATCG CAGGGCACCG TTGGTGGTCT CGTCGTAGCC 
GGTGTCATGC TGAAAACTAT CGGCTGGCGT GTCCTGGTGG GCGTGGGTGC CCTCTACGGC 
TGCATCTACC TGTACGAGCG CCTCTCGTGG ACAAATTCAG CCAAGGAGCG AACGTTCAAG 
TCGCAGTACG TGCGCCATGC CACCAAGAAG CTCAAGATGA TTGTCGACCT CACCTCGGCC 
AACTGCAGCC ACCAAGTGCA GCAGGAATTG TCGAGCACCT TTGCCCGTTT GTGCCGCACG 
GTTGATACCG CCACCACGGA CATGAATGAT GAGCTCAAGA CGCTGGACTC GCAGCTGAAC 
ATCCTGGAGG CGAACCAGAA GCAATTGAAG CTGCTTCGCA ACAAGGCCAA CTACATACAG 
AACGAGCTGG ACATCTTCGA GCACAACTAT ATATCGCCGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Marf cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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