wuho cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	wuho
Entrez Gene ID	31566
Full Name	CG15897 gene product from transcript CG15897-RA
Synonyms	CG15897,Dmel\CG15897,wh
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5E5-5E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_132079.4	NP_572307.1	wuho

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	WUHO_ANOGA	XP_001689350.1
Drosophila melanogaster (fruit fly)	wuho	NP_572307.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CG15897, here named wuho (wh) contains 5 WD40 repeats; has a critical function in cellular differentiation for germline cells during gametogenesis
Title: A new Drosophila gene wh (wuho) with WD40 repeats is essential for spermatogenesis and has maximal expression in hub cells.

The following wuho gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wuho cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03124	NM_132079.4 Latest version!	Drosophila melanogaster wuho (wuho), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wuho ( NM_132079.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572307.1 Drosophila melanogaster wuho (wuho), mRNA.

Price & Availability↑

CloneID	OFa03124
Clone ID Related Accession (Same CDS sequence)	NM_132079.4
Accession Version	NM_132079.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	wuho
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132079.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCACAA CAATTTCGTT CGCGGAACCC GAAATCGTGC TCGGCCATGG CCGCAGGGTG 
CTCTTCGTGA ACCCCGACGA CCTGCAGATA TTCAAGGAGA TCGAGCTGCC GCCCGACCTT 
GGCCTGAAAG GCCATACATC CCAGTCGCAG GAATCGTGTA CAGCCGCAGC AGCCGCATCC 
ACAGCCACAG CCGCATCCGG TCAGGCACCG GGTGGCAAGG AGCAACAGCT TGCCAATCAG 
CCAGAGGAGG GCGGAACCTC TGCCAGCGCC TCCGGTTTGG GATGTGCCAC CAGCACATCG 
GTACAGAATG TGGCCTACTC GCCAGACGGA CAGCTGCTGG CCGTGACCAC CAGTGGAAAA 
CAGAAAGCTC TCCTGCTCTA TCGTTCACGG CCGGAGAACG CCCGCCTGCT CTCCGCCCGC 
CCACTGGCCC GGGCGTCCAG TGCCCTGCGC TTCTGCAGCG ACAGCAGCTC CATTCTGGTC 
ACAGACAAGA CTGGCGATTG CTATCAGTAC GACTGCGTTG AGGTGGAGGC TCCGCCGCGC 
CTGCTGTTGG GCCATTTAAG TGTGGTATAC GACATCCTGT GGTCGGAGGA CCAGCAGCAT 
ATCATCACCT GCGATCGGGA TGATAAGATA CGCGTGACCA ACTATCCCGC CACCTTTGAC 
ATCCACAGCT ATTGCCTGGG CCACAGGGAG TTTGTCTCAG GCCTGGCACT GCTCACGGAG 
CAGCATATCG CCTCCGCTTC GGGGGACAAG ACCTTGCGCG TATGGAACTA CATTCAGGGC 
AAAGAGCTGC TGCAGCACGA GCTACCAGCA CCGGCGGTTC GTTTATTGGT ACGCCAGCTG 
GAGCCGGAGA AGGTCTTCCA GGCGGCGGTG CTCTTCTACG AGCACGTGGA CGCCCTGGGA 
TTGTATCGCC TGGAGCGCAG CTCCGATGAC ACCTGGAGCG TGACGGCCAC ACAACTGGTT 
TGCGCCGAAG CGGGCTCATG GAGCATTAGC AACTTTACTT TAACCAGCGA CCGGATCTAC 
ATCACCGGCG CAGAGAACGA ACGCTTAAGC TTGCGCGTCT ACGACATCGC AACTGGCCAG 
CCGGCGAGCA GCGGCGTGCC CGAGGGCTGG CTGAAGATGG TGCTAGACGG ATTGGGTGCC 
AACGAGGAGG GCGCACCGCC ATTCATTCCC GAGGACTTGT CCGTTTGGTT TAAGAAGCGC 
TTCGACAACG TTAGCGACTA TTTGGAGCGC AAGAAGCGGC GCATCGAGGA GCAGCAGCAG 
CAGAAGTGCG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572307.1
CDS	55..1329
Translation	MCTTISFAEPEIVLGHGRRVLFVNPDDLQIFKEIELPPDLGLKGHTSQSQESCTAAAAASTATAASGQAPGGKEQQLANQPEEGGTSASASGLGCATSTSVQNVAYSPDGQLLAVTTSGKQKALLLYRSRPENARLLSARPLARASSALRFCSDSSSILVTDKTGDCYQYDCVEVEAPPRLLLGHLSVVYDILWSEDQQHIITCDRDDKIRVTNYPATFDIHSYCLGHREFVSGLALLTEQHIASASGDKTLRVWNYIQGKELLQHELPAPAVRLLVRQLEPEKVFQAAVLFYEHVDALGLYRLERSSDDTWSVTATQLVCAEAGSWSISNFTLTSDRIYITGAENERLSLRVYDIATGQPASSGVPEGWLKMVLDGLGANEEGAPPFIPEDLSVWFKKRFDNVSDYLERKKRRIEEQQQQKCG

Target ORF information:

RefSeq Version	NM_132079.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster wuho (wuho), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132079.4

ORF Insert Sequence:

ATGTGCACAA CAATTTCGTT CGCGGAACCC GAAATCGTGC TCGGCCATGG CCGCAGGGTG 
CTCTTCGTGA ACCCCGACGA CCTGCAGATA TTCAAGGAGA TCGAGCTGCC GCCCGACCTT 
GGCCTGAAAG GCCATACATC CCAGTCGCAG GAATCGTGTA CAGCCGCAGC AGCCGCATCC 
ACAGCCACAG CCGCATCCGG TCAGGCACCG GGTGGCAAGG AGCAACAGCT TGCCAATCAG 
CCAGAGGAGG GCGGAACCTC TGCCAGCGCC TCCGGTTTGG GATGTGCCAC CAGCACATCG 
GTACAGAATG TGGCCTACTC GCCAGACGGA CAGCTGCTGG CCGTGACCAC CAGTGGAAAA 
CAGAAAGCTC TCCTGCTCTA TCGTTCACGG CCGGAGAACG CCCGCCTGCT CTCCGCCCGC 
CCACTGGCCC GGGCGTCCAG TGCCCTGCGC TTCTGCAGCG ACAGCAGCTC CATTCTGGTC 
ACAGACAAGA CTGGCGATTG CTATCAGTAC GACTGCGTTG AGGTGGAGGC TCCGCCGCGC 
CTGCTGTTGG GCCATTTAAG TGTGGTATAC GACATCCTGT GGTCGGAGGA CCAGCAGCAT 
ATCATCACCT GCGATCGGGA TGATAAGATA CGCGTGACCA ACTATCCCGC CACCTTTGAC 
ATCCACAGCT ATTGCCTGGG CCACAGGGAG TTTGTCTCAG GCCTGGCACT GCTCACGGAG 
CAGCATATCG CCTCCGCTTC GGGGGACAAG ACCTTGCGCG TATGGAACTA CATTCAGGGC 
AAAGAGCTGC TGCAGCACGA GCTACCAGCA CCGGCGGTTC GTTTATTGGT ACGCCAGCTG 
GAGCCGGAGA AGGTCTTCCA GGCGGCGGTG CTCTTCTACG AGCACGTGGA CGCCCTGGGA 
TTGTATCGCC TGGAGCGCAG CTCCGATGAC ACCTGGAGCG TGACGGCCAC ACAACTGGTT 
TGCGCCGAAG CGGGCTCATG GAGCATTAGC AACTTTACTT TAACCAGCGA CCGGATCTAC 
ATCACCGGCG CAGAGAACGA ACGCTTAAGC TTGCGCGTCT ACGACATCGC AACTGGCCAG 
CCGGCGAGCA GCGGCGTGCC CGAGGGCTGG CTGAAGATGG TGCTAGACGG ATTGGGTGCC 
AACGAGGAGG GCGCACCGCC ATTCATTCCC GAGGACTTGT CCGTTTGGTT TAAGAAGCGC 
TTCGACAACG TTAGCGACTA TTTGGAGCGC AAGAAGCGGC GCATCGAGGA GCAGCAGCAG 
CAGAAGTGCG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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wuho cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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