dtn cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following dtn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dtn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa00935 NM_001258618.2
Latest version!
Drosophila melanogaster detonator (dtn), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa00935 NM_132072.4
Latest version!
Drosophila melanogaster detonator (dtn), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa21376 NM_001298003.1
Latest version!
Drosophila melanogaster detonator (dtn), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa21377 NM_001298004.1
Latest version!
Drosophila melanogaster detonator (dtn), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa00935
    Clone ID Related Accession (Same CDS sequence) NM_001258618.2 , NM_132072.4
    Accession Version NM_001258618.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product detonator, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258618.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT
    AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC
    AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG
    CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG
    CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA
    CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG
    AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT
    CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT
    AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG
    GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC
    CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG
    CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT
    GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC
    AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG
    ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT
    CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001245547.1
    CDS529..4491
    Translation

    Target ORF information:

    RefSeq Version NM_001258618.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster detonator (dtn), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001258618.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT
    AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC
    AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG
    CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG
    CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA
    CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG
    AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT
    CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT
    AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG
    GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC
    CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG
    CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT
    GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC
    AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG
    ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT
    CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00935
    Clone ID Related Accession (Same CDS sequence) NM_001258618.2 , NM_132072.4
    Accession Version NM_132072.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product detonator, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132072.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT
    AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC
    AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG
    CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG
    CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA
    CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG
    AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT
    CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT
    AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG
    GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC
    CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG
    CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT
    GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC
    AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG
    ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT
    CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_572300.2
    CDS235..4197
    Translation

    Target ORF information:

    RefSeq Version NM_132072.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster detonator (dtn), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_132072.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT
    AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC
    AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG
    CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG
    CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA
    CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG
    AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT
    CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT
    AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG
    GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC
    CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG
    CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT
    GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC
    AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG
    ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT
    CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21376
    Clone ID Related Accession (Same CDS sequence) NM_001298003.1
    Accession Version NM_001298003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3966bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product detonator, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GCAGATTCCC GCATGCGCAT CACTAGCAAT CCGATTGTCA ACTACGACAA TGGACGCCGT
    GTTAGCTCCT TTGACCAACA GCCCAAGCTG CAGACACATA TCGTGCCGGC CTCCAATCTA
    AACAAGCAGC ACGCCGACCA CTATTTGGCC AACGAGCGCA AGGATAATGC CCTGGACTAC
    AAGCCACCAG TGCCGCCGCA CAGGAATGTG GGCGCTCGAG CCTGCCTGCC GGTACAGCCT
    GTGCTCGGTA CTGGATCTGT AAAGGATGCG GCACCCAACA AGCGACACAG TCAGCTGTAC
    AGACGTGAGC ATTTGCAGAA CAGCGATAGC AATGCCAATA GCCAACAGTC GACACCGCAG
    CCGAATCAGC AATTGCCAAC AGAACGCCCA CATCCACTGG CCCAAGCCCA TCCACATCCT
    CATCCACATC AGCATCAGCG CAGCCATAGC CATAGCCACA ACTTCAGTCA GGAGCCACTC
    ATTAAGGCGG CGCACAACAA AATCAAGCAG CAGCAACAAC AACAACAGCA GCAGCAACAC
    GAGGAGCTGC ACAACGCCGA GAAGCTGGTG GAGTACACGA ATCCGCACCA GAAGAACGCG
    TTCCAGTTCG ATTCGCTGAC ACCAAAGAGA GTTACCAAAG CAGCCGCGTC CTCGCCGGCA
    ACGCCGTCCA CATCAGCAGT CCAGGGCAAA GCTAAAGAAA ACCATAGCAT GGCCAGCAGC
    ACTGGGGATG CGGCCAACTC AAATGGAACG GACGAGATTA TAAGGCTGCC AGCCAGCGCC
    GTCAGCCAGG AGCCCACCAC GAAATCGTCG CGTGTTAAGC GCGCCACCGT GGTGGGCAAT
    CCGATGTTCT CGGCCACCGT CGATGAGTCT GTCGCCCCCG GAGAGCGTCT TGGCCTGGAC
    GATCTCGACA TGGATTACGA GCAGATCATG CACTACTTTG ACAACCTAAA GGAGTCAAAT
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284932.1
    CDS240..4205
    Translation

    Target ORF information:

    RefSeq Version NM_001298003.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster detonator (dtn), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GCAGATTCCC GCATGCGCAT CACTAGCAAT CCGATTGTCA ACTACGACAA TGGACGCCGT
    GTTAGCTCCT TTGACCAACA GCCCAAGCTG CAGACACATA TCGTGCCGGC CTCCAATCTA
    AACAAGCAGC ACGCCGACCA CTATTTGGCC AACGAGCGCA AGGATAATGC CCTGGACTAC
    AAGCCACCAG TGCCGCCGCA CAGGAATGTG GGCGCTCGAG CCTGCCTGCC GGTACAGCCT
    GTGCTCGGTA CTGGATCTGT AAAGGATGCG GCACCCAACA AGCGACACAG TCAGCTGTAC
    AGACGTGAGC ATTTGCAGAA CAGCGATAGC AATGCCAATA GCCAACAGTC GACACCGCAG
    CCGAATCAGC AATTGCCAAC AGAACGCCCA CATCCACTGG CCCAAGCCCA TCCACATCCT
    CATCCACATC AGCATCAGCG CAGCCATAGC CATAGCCACA ACTTCAGTCA GGAGCCACTC
    ATTAAGGCGG CGCACAACAA AATCAAGCAG CAGCAACAAC AACAACAGCA GCAGCAACAC
    GAGGAGCTGC ACAACGCCGA GAAGCTGGTG GAGTACACGA ATCCGCACCA GAAGAACGCG
    TTCCAGTTCG ATTCGCTGAC ACCAAAGAGA GTTACCAAAG CAGCCGCGTC CTCGCCGGCA
    ACGCCGTCCA CATCAGCAGT CCAGGGCAAA GCTAAAGAAA ACCATAGCAT GGCCAGCAGC
    ACTGGGGATG CGGCCAACTC AAATGGAACG GACGAGATTA TAAGGCTGCC AGCCAGCGCC
    GTCAGCCAGG AGCCCACCAC GAAATCGTCG CGTGTTAAGC GCGCCACCGT GGTGGGCAAT
    CCGATGTTCT CGGCCACCGT CGATGAGTCT GTCGCCCCCG GAGAGCGTCT TGGCCTGGAC
    GATCTCGACA TGGATTACGA GCAGATCATG CACTACTTTG ACAACCTAAA GGAGTCAAAT
    GCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa21377
    Clone ID Related Accession (Same CDS sequence) NM_001298004.1
    Accession Version NM_001298004.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4224bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product detonator, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT
    AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC
    AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG
    CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG
    CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA
    CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG
    AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT
    CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT
    AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG
    GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC
    CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG
    CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT
    GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC
    AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG
    ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT
    CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC
    TGAGTACAGG AGATCATTGC TAAAATTCGC GAGATATTGT CGACGTTCCA TCACCGATAT
    CAAACGGTTG CCACGCCCCT GACCTCGCCC ACTCGACAGC AGATGCAGCT GCTCGACGAA
    CTGGTCGCCG GCGTGGCAAC GGCCACAGCC ATCACCGCAG CCAAGCCAAC ACCATTGGTC
    CTGGATGGTG GAGAGGGAGA CGAGTCGGCT GATGCATTCG ATGCCTACGA TGGAGCGTTG
    GACATCGAGA GCAGGCAGTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001284933.1
    CDS240..4463
    Translation

    Target ORF information:

    RefSeq Version NM_001298004.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster detonator (dtn), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001298004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
    CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG
    CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC
    TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC
    ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG
    CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC
    CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG
    GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG
    CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA
    CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC
    TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA
    CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG
    CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG
    GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA
    CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC
    GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG
    GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC
    ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC
    AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC
    AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA
    AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC
    AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG
    GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC
    GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT
    GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG
    CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG
    TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC
    CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT
    CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG
    GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC
    AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA
    GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA
    TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG
    TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG
    AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC
    AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG
    TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG
    CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG
    GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT
    GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG
    TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT
    ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT
    GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA
    GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG
    CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA
    GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA
    ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT
    GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC
    TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA
    CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA
    GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT
    AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC
    AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG
    CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG
    CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA
    CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG
    AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT
    CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT
    AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG
    GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC
    CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG
    CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT
    GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC
    AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG
    ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT
    CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC
    TGAGTACAGG AGATCATTGC TAAAATTCGC GAGATATTGT CGACGTTCCA TCACCGATAT
    CAAACGGTTG CCACGCCCCT GACCTCGCCC ACTCGACAGC AGATGCAGCT GCTCGACGAA
    CTGGTCGCCG GCGTGGCAAC GGCCACAGCC ATCACCGCAG CCAAGCCAAC ACCATTGGTC
    CTGGATGGTG GAGAGGGAGA CGAGTCGGCT GATGCATTCG ATGCCTACGA TGGAGCGTTG
    GACATCGAGA GCAGGCAGTT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.