dtn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dtn
Entrez Gene ID	31555
Full Name	detonator
Synonyms	CG14446,Dmel\CG14446,MRE2,lincRNA.S8753
General protein information	Preferred Names detonator Names CG14446 gene product from transcript CG14446-RC CG14446-PB CG14446-PC CG14446-PD CG14446-PE dtn-PB dtn-PC dtn-PD dtn-PE mRNA-like ncRNA in embryogenesis 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5E1-5E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258618.2	NP_001245547.1	detonator, isoform C
NM_132072.4	NP_572300.2	detonator, isoform B
NM_001298003.1	NP_001284932.1	detonator, isoform D
NM_001298004.1	NP_001284933.1	detonator, isoform E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tmem132e	NP_001127882.1
Drosophila melanogaster (fruit fly)	CG14446	NP_001245547.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004670	XP_318358.5
Pan troglodytes (chimpanzee)	TMEM132E	XP_523601.3
Bos taurus (cattle)	TMEM132E	XP_002695701.2
Gallus gallus (chicken)	TMEM132E	XP_001234668.3
Danio rerio (zebrafish)	tmem132e	XP_692496.5
Mus musculus (house mouse)	Tmem132e	NP_075927.2
Homo sapiens (human)	TMEM132E	NP_997196.1
	TMEM132E	XP_001113490.1
Canis lupus familiaris (dog)	TMEM132E	XP_548267.3
Xenopus tropicalis (tropical clawed frog)	LOC100491853	XP_002940574.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dtn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dtn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00935	NM_001258618.2 Latest version!	Drosophila melanogaster detonator (dtn), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa00935	NM_132072.4 Latest version!	Drosophila melanogaster detonator (dtn), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21376	NM_001298003.1 Latest version!	Drosophila melanogaster detonator (dtn), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa21377	NM_001298004.1 Latest version!	Drosophila melanogaster detonator (dtn), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dtn ( NM_001258618.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245547.1 Drosophila melanogaster detonator (dtn), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00935
Clone ID Related Accession (Same CDS sequence)	NM_001258618.2 , NM_132072.4
Accession Version	NM_001258618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	detonator, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258618.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT 
AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC 
AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG 
CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG 
CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA 
CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG 
AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT 
CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT 
AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG 
GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC 
CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG 
CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT 
GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC 
AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG 
ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT 
CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245547.1
CDS	529..4491
Translation	MMRYCLIIALQLSLAYSVEVHFEAPDSGFFLKHARQPPVTPEIANVQTSSAVPPLRQRSSYDSVLSLDRFTVVETTQPVSIRASYGPFSTKQTVPARYIVPDTMDSQSGDYMNNATLLELQQPNMHLDISAHLVRSSVSQDAPVLRVLFHAGADPGGHLQRQKVCVLLHVAMGSEQPLKGRCMPEGEDGVCVAEVVVPLGWWPQLPAPTQDGSGQAPPKVPQRYAQVSYSVFEPPLRNPEQCEPKVQIQPLTTFAQVPLLAARTPYRMLRADDAVTFLLPQHPLYPLSRLHVPVFLHQYPDQRVAAFTVRARVKAGLKILGATASTDQWSVSVEKENPKHTTARVTAFRKEAESSLESGGNLTSEVFEVFSWLLEVAEDTNDIVDGGKIVWSVTHVYDTPKDKDSGELVIPDDNKKRLIAKLEINKDDIQAVLPMAKIWEVMNTAVLTGRQVAQAMKVFIVSQAGKVADVTLQSSCHAEDESVIKVSSSCSSVYVDGSEQRGSSNASVIVKYGTYVGVAKFIVWMPEFPLEVYISDFRLSQIKGWKVTDDNHYLHNKQSRRRKKRSYVWGQHGTNYYNNLGADKTICRARYQQSPVEVYAKFLAVDQNSGRTSYFISRRTGLRVTDLVQPLLRVADPKIASLKGRILQGKSMGRTDVQVLSPITGRVIGTKEIRVGSDKVNLSKLIVRVISGLQLTISPDNTIENGYLAETSVTHKLTAQYQEGLLDIDLEFTDGSKTPLRDIAVEDYFLLVESLDTEVVAFAPMLASHHPRVIAVGEGNGNLLRVTLLLSEECRQRRGTLSSSKQNKSNAAPLASALASIEVDFNNVDIVSKQEAIQNDGTVGRERKNYRQTGDLADIIVGIPLRDSSQLYEPTVQARQHRGSIQAVHKGHHGKGLAGTGTMTTMELGMYVLLTAFCFAIIVFVISCVVYASKFRPAMIESGLDPLSAGKSNGSGGSGGFRDVRLKESTTNAHDWVWLGRSTIDRQSIVAETNTHLNPRDSRMRITSNPIVNYDNGRRVSSFDQQPKLQTHIVPASNLNKQHADHYLANERKDNALDYKPPVPPHRNVGARACLPVQPVLGTGSVKDAAPNKRHSQLYRREHLQNSDSNANSQQSTPQPNQQLPTERPHPLAQAHPHPHPHQHQRSHSHSHNFSQEPLIKAAHNKIKQQQQQQQQQQHEELHNAEKLVEYTNPHQKNAFQFDSLTPKRVTKAAASSPATPSTSAVQGKAKENHSMASSTGDAANSNGTDEIIRLPASAVSQEPTTKSSRVKRATVVGNPMFSATVDESVAPGERLGLDDLDMDYEQIMHYFDNLKESNA

Target ORF information:

RefSeq Version	NM_001258618.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster detonator (dtn), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258618.2

ORF Insert Sequence:

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT 
AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC 
AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG 
CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG 
CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA 
CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG 
AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT 
CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT 
AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG 
GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC 
CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG 
CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT 
GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC 
AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG 
ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT 
CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dtn ( NM_132072.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572300.2 Drosophila melanogaster detonator (dtn), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00935
Clone ID Related Accession (Same CDS sequence)	NM_001258618.2 , NM_132072.4
Accession Version	NM_132072.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	detonator, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132072.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT 
AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC 
AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG 
CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG 
CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA 
CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG 
AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT 
CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT 
AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG 
GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC 
CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG 
CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT 
GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC 
AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG 
ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT 
CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572300.2
CDS	235..4197
Translation	MMRYCLIIALQLSLAYSVEVHFEAPDSGFFLKHARQPPVTPEIANVQTSSAVPPLRQRSSYDSVLSLDRFTVVETTQPVSIRASYGPFSTKQTVPARYIVPDTMDSQSGDYMNNATLLELQQPNMHLDISAHLVRSSVSQDAPVLRVLFHAGADPGGHLQRQKVCVLLHVAMGSEQPLKGRCMPEGEDGVCVAEVVVPLGWWPQLPAPTQDGSGQAPPKVPQRYAQVSYSVFEPPLRNPEQCEPKVQIQPLTTFAQVPLLAARTPYRMLRADDAVTFLLPQHPLYPLSRLHVPVFLHQYPDQRVAAFTVRARVKAGLKILGATASTDQWSVSVEKENPKHTTARVTAFRKEAESSLESGGNLTSEVFEVFSWLLEVAEDTNDIVDGGKIVWSVTHVYDTPKDKDSGELVIPDDNKKRLIAKLEINKDDIQAVLPMAKIWEVMNTAVLTGRQVAQAMKVFIVSQAGKVADVTLQSSCHAEDESVIKVSSSCSSVYVDGSEQRGSSNASVIVKYGTYVGVAKFIVWMPEFPLEVYISDFRLSQIKGWKVTDDNHYLHNKQSRRRKKRSYVWGQHGTNYYNNLGADKTICRARYQQSPVEVYAKFLAVDQNSGRTSYFISRRTGLRVTDLVQPLLRVADPKIASLKGRILQGKSMGRTDVQVLSPITGRVIGTKEIRVGSDKVNLSKLIVRVISGLQLTISPDNTIENGYLAETSVTHKLTAQYQEGLLDIDLEFTDGSKTPLRDIAVEDYFLLVESLDTEVVAFAPMLASHHPRVIAVGEGNGNLLRVTLLLSEECRQRRGTLSSSKQNKSNAAPLASALASIEVDFNNVDIVSKQEAIQNDGTVGRERKNYRQTGDLADIIVGIPLRDSSQLYEPTVQARQHRGSIQAVHKGHHGKGLAGTGTMTTMELGMYVLLTAFCFAIIVFVISCVVYASKFRPAMIESGLDPLSAGKSNGSGGSGGFRDVRLKESTTNAHDWVWLGRSTIDRQSIVAETNTHLNPRDSRMRITSNPIVNYDNGRRVSSFDQQPKLQTHIVPASNLNKQHADHYLANERKDNALDYKPPVPPHRNVGARACLPVQPVLGTGSVKDAAPNKRHSQLYRREHLQNSDSNANSQQSTPQPNQQLPTERPHPLAQAHPHPHPHQHQRSHSHSHNFSQEPLIKAAHNKIKQQQQQQQQQQHEELHNAEKLVEYTNPHQKNAFQFDSLTPKRVTKAAASSPATPSTSAVQGKAKENHSMASSTGDAANSNGTDEIIRLPASAVSQEPTTKSSRVKRATVVGNPMFSATVDESVAPGERLGLDDLDMDYEQIMHYFDNLKESNA

Target ORF information:

RefSeq Version	NM_132072.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster detonator (dtn), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132072.4

ORF Insert Sequence:

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT 
AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC 
AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG 
CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG 
CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA 
CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG 
AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT 
CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT 
AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG 
GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC 
CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG 
CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT 
GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC 
AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG 
ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT 
CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dtn ( NM_001298003.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284932.1 Drosophila melanogaster detonator (dtn), transcript variant D, mRNA.

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CloneID	OFa21376
Clone ID Related Accession (Same CDS sequence)	NM_001298003.1
Accession Version	NM_001298003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	detonator, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GCAGATTCCC GCATGCGCAT CACTAGCAAT CCGATTGTCA ACTACGACAA TGGACGCCGT 
GTTAGCTCCT TTGACCAACA GCCCAAGCTG CAGACACATA TCGTGCCGGC CTCCAATCTA 
AACAAGCAGC ACGCCGACCA CTATTTGGCC AACGAGCGCA AGGATAATGC CCTGGACTAC 
AAGCCACCAG TGCCGCCGCA CAGGAATGTG GGCGCTCGAG CCTGCCTGCC GGTACAGCCT 
GTGCTCGGTA CTGGATCTGT AAAGGATGCG GCACCCAACA AGCGACACAG TCAGCTGTAC 
AGACGTGAGC ATTTGCAGAA CAGCGATAGC AATGCCAATA GCCAACAGTC GACACCGCAG 
CCGAATCAGC AATTGCCAAC AGAACGCCCA CATCCACTGG CCCAAGCCCA TCCACATCCT 
CATCCACATC AGCATCAGCG CAGCCATAGC CATAGCCACA ACTTCAGTCA GGAGCCACTC 
ATTAAGGCGG CGCACAACAA AATCAAGCAG CAGCAACAAC AACAACAGCA GCAGCAACAC 
GAGGAGCTGC ACAACGCCGA GAAGCTGGTG GAGTACACGA ATCCGCACCA GAAGAACGCG 
TTCCAGTTCG ATTCGCTGAC ACCAAAGAGA GTTACCAAAG CAGCCGCGTC CTCGCCGGCA 
ACGCCGTCCA CATCAGCAGT CCAGGGCAAA GCTAAAGAAA ACCATAGCAT GGCCAGCAGC 
ACTGGGGATG CGGCCAACTC AAATGGAACG GACGAGATTA TAAGGCTGCC AGCCAGCGCC 
GTCAGCCAGG AGCCCACCAC GAAATCGTCG CGTGTTAAGC GCGCCACCGT GGTGGGCAAT 
CCGATGTTCT CGGCCACCGT CGATGAGTCT GTCGCCCCCG GAGAGCGTCT TGGCCTGGAC 
GATCTCGACA TGGATTACGA GCAGATCATG CACTACTTTG ACAACCTAAA GGAGTCAAAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284932.1
CDS	240..4205
Translation	MMRYCLIIALQLSLAYSVEVHFEAPDSGFFLKHARQPPVTPEIANVQTSSAVPPLRQRSSYDSVLSLDRFTVVETTQPVSIRASYGPFSTKQTVPARYIVPDTMDSQSGDYMNNATLLELQQPNMHLDISAHLVRSSVSQDAPVLRVLFHAGADPGGHLQRQKVCVLLHVAMGSEQPLKGRCMPEGEDGVCVAEVVVPLGWWPQLPAPTQDGSGQAPPKVPQRYAQVSYSVFEPPLRNPEQCEPKVQIQPLTTFAQVPLLAARTPYRMLRADDAVTFLLPQHPLYPLSRLHVPVFLHQYPDQRVAAFTVRARVKAGLKILGATASTDQWSVSVEKENPKHTTARVTAFRKEAESSLESGGNLTSEVFEVFSWLLEVAEDTNDIVDGGKIVWSVTHVYDTPKDKDSGELVIPDDNKKRLIAKLEINKDDIQAVLPMAKIWEVMNTAVLTGRQVAQAMKVFIVSQAGKVADVTLQSSCHAEDESVIKVSSSCSSVYVDGSEQRGSSNASVIVKYGTYVGVAKFIVWMPEFPLEVYISDFRLSQIKGWKVTDDNHYLHNKQSRRRKKRSYVWGQHGTNYYNNLGADKTICRARYQQSPVEVYAKFLAVDQNSGRTSYFISRRTGLRVTDLVQPLLRVADPKIASLKGRILQGKSMGRTDVQVLSPITGRVIGTKEIRVGSDKVNLSKLIVRVISGLQLTISPDNTIENGYLAETSVTHKLTAQYQEGLLDIDLEFTDGSKTPLRDIAVEDYFLLVESLDTEVVAFAPMLASHHPRVIAVGEGNGNLLRVTLLLSEECRQRRGTLSSSKQNKSNAAPLASALASIEVDFNNVDIVSKQEAIQNDGTVGRERKNYRQTGDLADIIVGIPLRDSSQLYEPTVQARQHRGSIQAVHKGHHGKGLAGTGTMTTMELGMYVLLTAFCFAIIVFVISCVVYASKFRPAMIESGLDPLSAGKSNGSGGSGGFRDVRLKESTTNAHDWVWLGRSTIDRQSIVAETNTHLNPRADSRMRITSNPIVNYDNGRRVSSFDQQPKLQTHIVPASNLNKQHADHYLANERKDNALDYKPPVPPHRNVGARACLPVQPVLGTGSVKDAAPNKRHSQLYRREHLQNSDSNANSQQSTPQPNQQLPTERPHPLAQAHPHPHPHQHQRSHSHSHNFSQEPLIKAAHNKIKQQQQQQQQQQHEELHNAEKLVEYTNPHQKNAFQFDSLTPKRVTKAAASSPATPSTSAVQGKAKENHSMASSTGDAANSNGTDEIIRLPASAVSQEPTTKSSRVKRATVVGNPMFSATVDESVAPGERLGLDDLDMDYEQIMHYFDNLKESNA

Target ORF information:

RefSeq Version	NM_001298003.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster detonator (dtn), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298003.1

ORF Insert Sequence:

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GCAGATTCCC GCATGCGCAT CACTAGCAAT CCGATTGTCA ACTACGACAA TGGACGCCGT 
GTTAGCTCCT TTGACCAACA GCCCAAGCTG CAGACACATA TCGTGCCGGC CTCCAATCTA 
AACAAGCAGC ACGCCGACCA CTATTTGGCC AACGAGCGCA AGGATAATGC CCTGGACTAC 
AAGCCACCAG TGCCGCCGCA CAGGAATGTG GGCGCTCGAG CCTGCCTGCC GGTACAGCCT 
GTGCTCGGTA CTGGATCTGT AAAGGATGCG GCACCCAACA AGCGACACAG TCAGCTGTAC 
AGACGTGAGC ATTTGCAGAA CAGCGATAGC AATGCCAATA GCCAACAGTC GACACCGCAG 
CCGAATCAGC AATTGCCAAC AGAACGCCCA CATCCACTGG CCCAAGCCCA TCCACATCCT 
CATCCACATC AGCATCAGCG CAGCCATAGC CATAGCCACA ACTTCAGTCA GGAGCCACTC 
ATTAAGGCGG CGCACAACAA AATCAAGCAG CAGCAACAAC AACAACAGCA GCAGCAACAC 
GAGGAGCTGC ACAACGCCGA GAAGCTGGTG GAGTACACGA ATCCGCACCA GAAGAACGCG 
TTCCAGTTCG ATTCGCTGAC ACCAAAGAGA GTTACCAAAG CAGCCGCGTC CTCGCCGGCA 
ACGCCGTCCA CATCAGCAGT CCAGGGCAAA GCTAAAGAAA ACCATAGCAT GGCCAGCAGC 
ACTGGGGATG CGGCCAACTC AAATGGAACG GACGAGATTA TAAGGCTGCC AGCCAGCGCC 
GTCAGCCAGG AGCCCACCAC GAAATCGTCG CGTGTTAAGC GCGCCACCGT GGTGGGCAAT 
CCGATGTTCT CGGCCACCGT CGATGAGTCT GTCGCCCCCG GAGAGCGTCT TGGCCTGGAC 
GATCTCGACA TGGATTACGA GCAGATCATG CACTACTTTG ACAACCTAAA GGAGTCAAAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dtn ( NM_001298004.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284933.1 Drosophila melanogaster detonator (dtn), transcript variant E, mRNA.

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CloneID	OFa21377
Clone ID Related Accession (Same CDS sequence)	NM_001298004.1
Accession Version	NM_001298004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	detonator, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT 
AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC 
AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG 
CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG 
CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA 
CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG 
AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT 
CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT 
AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG 
GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC 
CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG 
CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT 
GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC 
AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG 
ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT 
CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC 
TGAGTACAGG AGATCATTGC TAAAATTCGC GAGATATTGT CGACGTTCCA TCACCGATAT 
CAAACGGTTG CCACGCCCCT GACCTCGCCC ACTCGACAGC AGATGCAGCT GCTCGACGAA 
CTGGTCGCCG GCGTGGCAAC GGCCACAGCC ATCACCGCAG CCAAGCCAAC ACCATTGGTC 
CTGGATGGTG GAGAGGGAGA CGAGTCGGCT GATGCATTCG ATGCCTACGA TGGAGCGTTG 
GACATCGAGA GCAGGCAGTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284933.1
CDS	240..4463
Translation	MMRYCLIIALQLSLAYSVEVHFEAPDSGFFLKHARQPPVTPEIANVQTSSAVPPLRQRSSYDSVLSLDRFTVVETTQPVSIRASYGPFSTKQTVPARYIVPDTMDSQSGDYMNNATLLELQQPNMHLDISAHLVRSSVSQDAPVLRVLFHAGADPGGHLQRQKVCVLLHVAMGSEQPLKGRCMPEGEDGVCVAEVVVPLGWWPQLPAPTQDGSGQAPPKVPQRYAQVSYSVFEPPLRNPEQCEPKVQIQPLTTFAQVPLLAARTPYRMLRADDAVTFLLPQHPLYPLSRLHVPVFLHQYPDQRVAAFTVRARVKAGLKILGATASTDQWSVSVEKENPKHTTARVTAFRKEAESSLESGGNLTSEVFEVFSWLLEVAEDTNDIVDGGKIVWSVTHVYDTPKDKDSGELVIPDDNKKRLIAKLEINKDDIQAVLPMAKIWEVMNTAVLTGRQVAQAMKVFIVSQAGKVADVTLQSSCHAEDESVIKVSSSCSSVYVDGSEQRGSSNASVIVKYGTYVGVAKFIVWMPEFPLEVYISDFRLSQIKGWKVTDDNHYLHNKQSRRRKKRSYVWGQHGTNYYNNLGADKTICRARYQQSPVEVYAKFLAVDQNSGRTSYFISRRTGLRVTDLVQPLLRVADPKIASLKGRILQGKSMGRTDVQVLSPITGRVIGTKEIRVGSDKVNLSKLIVRVISGLQLTISPDNTIENGYLAETSVTHKLTAQYQEGLLDIDLEFTDGSKTPLRDIAVEDYFLLVESLDTEVVAFAPMLASHHPRVIAVGEGNGNLLRVTLLLSEECRQRRGTLSSSKQNKSNAAPLASALASIEVDFNNVDIVSKQEAIQNDGTVGRERKNYRQTGDLADIIVGIPLRDSSQLYEPTVQARQHRGSIQAVHKGHHGKGLAGTGTMTTMELGMYVLLTAFCFAIIVFVISCVVYASKFRPAMIESGLDPLSAGKSNGSGGSGGFRDVRLKESTTNAHDWVWLGRSTIDRQSIVAETNTHLNPRDSRMRITSNPIVNYDNGRRVSSFDQQPKLQTHIVPASNLNKQHADHYLANERKDNALDYKPPVPPHRNVGARACLPVQPVLGTGSVKDAAPNKRHSQLYRREHLQNSDSNANSQQSTPQPNQQLPTERPHPLAQAHPHPHPHQHQRSHSHSHNFSQEPLIKAAHNKIKQQQQQQQQQQHEELHNAEKLVEYTNPHQKNAFQFDSLTPKRVTKAAASSPATPSTSAVQGKAKENHSMASSTGDAANSNGTDEIIRLPASAVSQEPTTKSSRVKRATVVGNPMFSATVDESVAPGERLGLDDLDMDYEQIMHYFDNLKESNA*VQEIIAKIREILSTFHHRYQTVATPLTSPTRQQMQLLDELVAGVATATAITAAKPTPLVLDGGEGDESADAFDAYDGALDIESRQF

Target ORF information:

RefSeq Version	NM_001298004.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster detonator (dtn), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298004.1

ORF Insert Sequence:

ATGATGAGAT ACTGCCTCAT AATTGCTTTG CAGCTGTCGT TGGCATACTC CGTGGAGGTG 
CACTTCGAGG CGCCGGACAG CGGCTTCTTT CTGAAGCACG CCCGCCAACC ACCTGTGACG 
CCGGAGATCG CCAATGTGCA GACATCCTCG GCGGTGCCGC CGTTACGGCA ACGATCCTCC 
TATGATTCCG TGTTATCTCT GGACAGATTC ACTGTGGTCG AGACCACGCA GCCGGTGTCC 
ATACGCGCCA GCTATGGACC GTTTTCCACG AAACAAACGG TGCCAGCCCG CTACATTGTG 
CCGGACACAA TGGACAGCCA GTCGGGCGAT TATATGAACA ATGCCACTCT ACTGGAGCTC 
CAGCAGCCAA ATATGCACCT GGACATATCC GCCCATTTGG TTCGCTCCAG TGTATCGCAG 
GATGCTCCCG TGCTTCGGGT ACTATTCCAC GCCGGAGCCG ATCCGGGTGG TCATTTGCAG 
CGCCAGAAGG TCTGTGTACT CCTGCACGTG GCGATGGGTT CGGAGCAGCC GCTCAAAGGA 
CGCTGCATGC CGGAGGGCGA GGACGGTGTC TGCGTAGCCG AAGTGGTCGT GCCCCTGGGC 
TGGTGGCCCC AGCTGCCGGC GCCCACACAA GATGGCAGTG GTCAGGCGCC ACCCAAAGTA 
CCGCAGCGGT ATGCCCAGGT CTCGTACAGT GTCTTTGAAC CGCCTTTGCG TAATCCCGAG 
CAATGCGAGC CGAAGGTGCA GATCCAACCG TTGACCACAT TCGCCCAGGT GCCTTTGTTG 
GCCGCCAGGA CTCCCTATCG TATGTTGCGC GCCGATGATG CCGTCACCTT TCTCCTGCCA 
CAACATCCGC TGTATCCACT CTCCCGTCTA CATGTGCCCG TCTTTTTACA TCAATATCCC 
GATCAGCGGG TGGCCGCCTT CACTGTGAGA GCTCGTGTTA AGGCGGGCTT AAAGATTCTG 
GGCGCCACCG CCTCCACAGA CCAATGGAGC GTTTCTGTAG AGAAGGAAAA TCCCAAACAC 
ACTACCGCTC GCGTTACTGC TTTCCGCAAA GAGGCCGAAT CCAGTCTGGA AAGTGGTGGC 
AACCTAACCA GCGAGGTGTT TGAAGTATTC TCATGGCTGC TCGAGGTGGC CGAGGACACC 
AATGATATTG TGGACGGTGG CAAGATTGTG TGGTCCGTGA CGCATGTCTA TGACACGCCA 
AAGGACAAGG ATTCCGGGGA ACTGGTTATA CCCGATGACA ACAAGAAGCG ACTAATTGCC 
AAGCTGGAGA TCAACAAGGA TGACATTCAG GCGGTGCTAC CGATGGCCAA AATCTGGGAG 
GTGATGAACA CGGCGGTGCT AACGGGCAGA CAGGTGGCCC AGGCGATGAA GGTGTTCATC 
GTTTCGCAGG CAGGCAAGGT GGCCGATGTG ACGCTACAGA GCTCCTGTCA CGCCGAAGAT 
GAAAGTGTAA TCAAGGTCTC CTCCTCCTGC AGCTCTGTTT ATGTGGATGG CTCCGAGCAG 
CGCGGCTCAT CCAATGCCTC GGTCATTGTC AAGTATGGCA CCTATGTGGG CGTGGCCAAG 
TTCATCGTTT GGATGCCCGA GTTTCCGCTG GAAGTTTACA TATCCGATTT CCGTCTCTCC 
CAAATCAAAG GATGGAAAGT AACCGATGAC AATCACTATC TACACAACAA GCAGTCTCGT 
CGAAGAAAGA AGAGATCCTA CGTTTGGGGT CAGCATGGAA CGAACTACTA TAACAATCTG 
GGAGCGGACA AGACGATTTG TCGCGCTCGC TACCAGCAAA GTCCCGTGGA GGTGTATGCC 
AAGTTCCTGG CCGTGGACCA GAACTCTGGA CGCACCAGCT ACTTTATCTC ACGGCGTACA 
GGCTTAAGAG TGACGGATCT GGTGCAACCG CTGCTCCGTG TGGCCGATCC CAAGATCGCA 
TCTCTGAAGG GTCGAATTCT GCAGGGCAAG TCCATGGGAC GCACCGATGT CCAGGTCCTG 
TCACCCATAA CGGGTCGAGT AATTGGCACC AAGGAAATCC GTGTGGGCAG CGACAAGGTG 
AACCTTTCCA AGCTCATCGT GCGCGTTATT TCGGGTCTGC AGCTTACCAT CAGTCCGGAC 
AACACGATTG AGAACGGCTA CCTGGCGGAG ACTTCTGTCA CACACAAGCT AACTGCTCAG 
TACCAGGAGG GATTGCTTGA CATCGATCTG GAGTTTACCG ATGGCTCCAA AACTCCTTTG 
CGAGATATTG CCGTGGAGGA CTACTTCCTG CTGGTGGAGA GTTTGGATAC CGAGGTGGTG 
GCATTTGCTC CCATGCTGGC TTCCCATCAT CCACGTGTCA TAGCCGTCGG CGAGGGCAAT 
GGTAACCTGC TGCGCGTGAC CCTTCTATTA TCCGAGGAAT GCCGTCAGCG ACGTGGCACG 
TTGAGCAGCT CCAAACAGAA CAAATCGAAT GCAGCACCAT TGGCCAGTGC ATTGGCATCT 
ATTGAAGTGG ATTTCAACAA TGTGGATATC GTGAGCAAGC AGGAGGCTAT CCAAAATGAT 
GGCACTGTGG GCAGGGAGAG AAAGAACTAT AGGCAAACAG GAGATCTGGC CGATATTATA 
GTGGGCATCC CCTTGAGGGA CTCCAGCCAG CTGTACGAGC CTACTGTTCA GGCGCGTCAG 
CATCGGGGCA GCATTCAGGC GGTTCATAAG GGTCATCATG GCAAGGGACT AGCTGGCACA 
GGCACCATGA CCACCATGGA ACTGGGCATG TACGTTCTGC TCACGGCGTT CTGCTTCGCA 
ATTATTGTGT TTGTGATCTC TTGCGTTGTG TATGCCTCCA AATTTCGGCC AGCAATGATT 
GAATCTGGCT TGGATCCTCT GTCGGCGGGC AAGAGCAATG GTTCTGGTGG ATCGGGTGGC 
TTCCGGGATG TGCGCCTGAA GGAGTCGACA ACGAATGCAC ACGATTGGGT CTGGCTGGGA 
CGCTCCACCA TCGACCGCCA GTCCATTGTG GCCGAGACCA ACACGCATCT GAATCCGAGA 
GATTCCCGCA TGCGCATCAC TAGCAATCCG ATTGTCAACT ACGACAATGG ACGCCGTGTT 
AGCTCCTTTG ACCAACAGCC CAAGCTGCAG ACACATATCG TGCCGGCCTC CAATCTAAAC 
AAGCAGCACG CCGACCACTA TTTGGCCAAC GAGCGCAAGG ATAATGCCCT GGACTACAAG 
CCACCAGTGC CGCCGCACAG GAATGTGGGC GCTCGAGCCT GCCTGCCGGT ACAGCCTGTG 
CTCGGTACTG GATCTGTAAA GGATGCGGCA CCCAACAAGC GACACAGTCA GCTGTACAGA 
CGTGAGCATT TGCAGAACAG CGATAGCAAT GCCAATAGCC AACAGTCGAC ACCGCAGCCG 
AATCAGCAAT TGCCAACAGA ACGCCCACAT CCACTGGCCC AAGCCCATCC ACATCCTCAT 
CCACATCAGC ATCAGCGCAG CCATAGCCAT AGCCACAACT TCAGTCAGGA GCCACTCATT 
AAGGCGGCGC ACAACAAAAT CAAGCAGCAG CAACAACAAC AACAGCAGCA GCAACACGAG 
GAGCTGCACA ACGCCGAGAA GCTGGTGGAG TACACGAATC CGCACCAGAA GAACGCGTTC 
CAGTTCGATT CGCTGACACC AAAGAGAGTT ACCAAAGCAG CCGCGTCCTC GCCGGCAACG 
CCGTCCACAT CAGCAGTCCA GGGCAAAGCT AAAGAAAACC ATAGCATGGC CAGCAGCACT 
GGGGATGCGG CCAACTCAAA TGGAACGGAC GAGATTATAA GGCTGCCAGC CAGCGCCGTC 
AGCCAGGAGC CCACCACGAA ATCGTCGCGT GTTAAGCGCG CCACCGTGGT GGGCAATCCG 
ATGTTCTCGG CCACCGTCGA TGAGTCTGTC GCCCCCGGAG AGCGTCTTGG CCTGGACGAT 
CTCGACATGG ATTACGAGCA GATCATGCAC TACTTTGACA ACCTAAAGGA GTCAAATGCC 
TGAGTACAGG AGATCATTGC TAAAATTCGC GAGATATTGT CGACGTTCCA TCACCGATAT 
CAAACGGTTG CCACGCCCCT GACCTCGCCC ACTCGACAGC AGATGCAGCT GCTCGACGAA 
CTGGTCGCCG GCGTGGCAAC GGCCACAGCC ATCACCGCAG CCAAGCCAAC ACCATTGGTC 
CTGGATGGTG GAGAGGGAGA CGAGTCGGCT GATGCATTCG ATGCCTACGA TGGAGCGTTG 
GACATCGAGA GCAGGCAGTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dtn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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