sqh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sqh
Entrez Gene ID	31554
Full Name	spaghetti squash
Synonyms	CG3595,DmMRLC_C,Dmel\CG3595,MLC,MLRC,MRLC,Myo,Myo II,Myo-II,Myo-II RLC,MyoII,NMM,RLC,RLC2,RMLC,Sqh,myo II,myoII,myosin,myosin II,sgh,spg,squ,ssq
General protein information	Preferred Names spaghetti squash Names CG3595 gene product from transcript CG3595-RA CG3595-PA CG3595-PB CG3595-PC CG3595-PD CG3595-PE Myo-II regulatory light chain MyoII regulatory light chain Myosin-II Myosin-II light chain Spaghetti-Squash light chain of nonmuscle myosin II myosin myosin 2 myosin II myosin II regulatory light chain myosin LC myosin light chain myosin regulatory light chain myosinII non-muscle myosin II non-muscle myosin II regulatory light chain non-muscle myosin-II nonmuscle myosin II nonmuscle myosin II regulatory light chain phospho-Myosin light chain 2 regulatory light chain regulatory light chain of myosin regulatory light chain of myosin II regulatory light chain of nonmuscle myosin II regulatory light chain of nonmuscle myosin-II regulatory myosin light chain sqh-PA sqh-PB sqh-PC sqh-PD sqh-PE squash type II myosin regulatory light chain
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5E1-5E1\|1-13 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298001.1	NP_001284930.1	spaghetti squash, isoform D
NM_001297999.1	NP_001284928.1	spaghetti squash, isoform B
NM_078502.3	NP_511057.1	spaghetti squash, isoform A
NM_001298002.1	NP_001284931.1	spaghetti squash, isoform E
NM_001298000.1	NP_001284929.1	spaghetti squash, isoform C

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MYL12A	NP_001015640.1
Caenorhabditis elegans (roundworm)	mlc-4	NP_497700.1
Mus musculus (house mouse)	Myl12a	NP_080340.2
Danio rerio (zebrafish)	myl12.2	NP_001124061.1
Gallus gallus (chicken)	MYL12A	NP_990672.1
Drosophila melanogaster (fruit fly)	sqh	NP_511057.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000927	XP_003437136.1
Xenopus tropicalis (tropical clawed frog)	myl12b	NP_001004813.1
Homo sapiens (human)	MYL12B	NP_291024.1
Pan troglodytes (chimpanzee)	MYL12B	XP_512040.1
	MYL12B	XP_001084275.1
Canis lupus familiaris (dog)	MYL12A	XP_848839.1
Rattus norvegicus (Norway rat)	Myl12a	NP_001128489.1
Gallus gallus (chicken)	LOC770011	XP_004939810.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mechanical forces regulate myosin dynamics during embryonic wound repair.
Title: Tension regulates myosin dynamics during

the regulation of NMII takes place downstream of guidance receptor signalling and is critical to ensure efficient collective migration.
Title: Myosin II governs collective cell migration behaviour downstream of guidance receptor signalling.

Computer simulation coupled with laser ablation suggests that Dunk-dependent maintenance of cortical myosin enables mechanical tension build-up, thereby providing a mechanism to guide myosin flow and define the hexagonal symmetry of the furrows.
Title: Flow-dependent myosin recruitment during Drosophila cellularization requires zygotic dunk activity.

Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis
Title: Modular activation of Rho1 by GPCR signalling imparts polarized myosin II activation during morphogenesis.

flw is a key regulator of basal myosin oscillations and cell contractions underlying egg chamber elongation.
Title: Myosin light-chain phosphatase regulates basal actomyosin oscillations during morphogenesis.

The following sqh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sqh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03156	NM_001298002.1 Latest version!	Drosophila melanogaster spaghetti squash (sqh), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa03156	NM_001298001.1 Latest version!	Drosophila melanogaster spaghetti squash (sqh), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa03156	NM_001298000.1 Latest version!	Drosophila melanogaster spaghetti squash (sqh), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa03156	NM_001297999.1 Latest version!	Drosophila melanogaster spaghetti squash (sqh), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa03156	NM_078502.3 Latest version!	Drosophila melanogaster spaghetti squash (sqh), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sqh ( NM_001298001.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284930.1 Drosophila melanogaster spaghetti squash (sqh), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03156
Clone ID Related Accession (Same CDS sequence)	NM_001298001.1 , NM_001297999.1 , NM_078502.3 , NM_001298002.1 , NM_001298000.1
Accession Version	NM_001298001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti squash, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284930.1
CDS	227..751
Translation	MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKDEQ

Target ORF information:

RefSeq Version	NM_001298001.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti squash (sqh), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298001.1

ORF Insert Sequence:

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqh ( NM_001297999.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284928.1 Drosophila melanogaster spaghetti squash (sqh), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03156
Clone ID Related Accession (Same CDS sequence)	NM_001298001.1 , NM_001297999.1 , NM_078502.3 , NM_001298002.1 , NM_001298000.1
Accession Version	NM_001297999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti squash, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284928.1
CDS	223..747
Translation	MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKDEQ

Target ORF information:

RefSeq Version	NM_001297999.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti squash (sqh), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297999.1

ORF Insert Sequence:

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqh ( NM_078502.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511057.1 Drosophila melanogaster spaghetti squash (sqh), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03156
Clone ID Related Accession (Same CDS sequence)	NM_001298001.1 , NM_001297999.1 , NM_078502.3 , NM_001298002.1 , NM_001298000.1
Accession Version	NM_078502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti squash, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078502.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511057.1
CDS	247..771
Translation	MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKDEQ

Target ORF information:

RefSeq Version	NM_078502.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti squash (sqh), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078502.3

ORF Insert Sequence:

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqh ( NM_001298002.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284931.1 Drosophila melanogaster spaghetti squash (sqh), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa03156
Clone ID Related Accession (Same CDS sequence)	NM_001298001.1 , NM_001297999.1 , NM_078502.3 , NM_001298002.1 , NM_001298000.1
Accession Version	NM_001298002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti squash, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284931.1
CDS	154..678
Translation	MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKDEQ

Target ORF information:

RefSeq Version	NM_001298002.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti squash (sqh), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298002.1

ORF Insert Sequence:

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sqh ( NM_001298000.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284929.1 Drosophila melanogaster spaghetti squash (sqh), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa03156
Clone ID Related Accession (Same CDS sequence)	NM_001298001.1 , NM_001297999.1 , NM_078502.3 , NM_001298002.1 , NM_001298000.1
Accession Version	NM_001298000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spaghetti squash, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284929.1
CDS	533..1057
Translation	MSSRKTAGRRATTKKRAQRATSNVFAMFDQAQIAEFKEAFNMIDQNRDGFVEKEDLHDMLASLGKNPTDDYLDGMMNEAPGPINFTMFLTLFGERLQGTDPEDVIKNAFGCFDEENMGVLPEDRLRELLTTMGDRFTDEDVDEMYREAPIKNGLFDYLEFTRILKHGAKDKDEQ

Target ORF information:

RefSeq Version	NM_001298000.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spaghetti squash (sqh), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298000.1

ORF Insert Sequence:

ATGTCATCCC GTAAGACCGC CGGACGTCGC GCCACCACCA AGAAGCGCGC CCAACGCGCC 
ACCTCCAATG TGTTCGCCAT GTTCGATCAG GCGCAGATTG CCGAGTTCAA GGAGGCATTC 
AACATGATCG ACCAGAACCG TGATGGCTTC GTCGAGAAGG AGGATCTGCA CGACATGCTG 
GCCTCGCTGG GCAAGAACCC AACGGACGAC TATCTCGATG GCATGATGAA CGAGGCGCCC 
GGGCCCATCA ACTTCACCAT GTTCCTCACG CTCTTCGGCG AGCGGCTGCA GGGCACCGAT 
CCCGAGGATG TGATCAAGAA CGCCTTCGGC TGCTTCGACG AGGAGAATAT GGGCGTCCTG 
CCCGAGGATC GCCTGAGGGA GCTGCTGACC ACCATGGGTG ATCGGTTCAC AGACGAGGAT 
GTGGACGAGA TGTATCGGGA GGCGCCCATC AAAAACGGTC TGTTCGACTA CCTCGAATTC 
ACGCGCATCC TTAAGCACGG TGCCAAGGAC AAGGATGAGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sqh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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