Efr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Efr
Entrez Gene ID	31553
Full Name	ER GDP-fucose transporter
Synonyms	CG3774,Dmel\CG3774
General protein information	Preferred Names ER GDP-fucose transporter Names CG3774 gene product from transcript CG3774-RA CG3774-PA CG3774-PB ER GFP-fucose transporter Efr-PA Efr-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 5E1-5E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_167062.3	NP_727071.1	ER GDP-fucose transporter, isoform B
NM_132071.3	NP_572299.1	ER GDP-fucose transporter, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc35b4	NP_067410.1
Caenorhabditis elegans (roundworm)	nstp-2	NP_001040936.1
	SLC35B4	XP_001100494.1
Bos taurus (cattle)	SLC35B4	NP_001179870.1
Canis lupus familiaris (dog)	SLC35B4	NP_001171932.1
Rattus norvegicus (Norway rat)	Slc35b4	NP_001100060.1
Danio rerio (zebrafish)	slc35b4	NP_997817.1
Drosophila melanogaster (fruit fly)	Efr	NP_572299.1
Xenopus tropicalis (tropical clawed frog)	slc35b4	XP_002931893.1
Homo sapiens (human)	SLC35B4	NP_116215.1
Pan troglodytes (chimpanzee)	SLC35B4	XP_519393.1
Gallus gallus (chicken)	SLC35B4	XP_414994.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011493	XP_317807.4
Kluyveromyces lactis	KLLA0B03157g	XP_451673.1
Saccharomyces cerevisiae (baker's yeast)	YEA4	NP_010912.1
Schizosaccharomyces pombe (fission yeast)	SPBC1734.09	NP_595426.1
Magnaporthe oryzae (rice blast fungus)	MGG_05631	XP_003710519.1
Neurospora crassa	NCU02980	XP_965367.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Drosophila CG3774 gene has been identified as a putative ortholog of human SLC35B4, which encodes a UDP-GlcNAc/UDP-xylose transporter, and was called as Efr (ER GDP-fucose transporter).
Title: Two pathways for importing GDP-fucose into the endoplasmic reticulum lumen function redundantly in the O-fucosylation of Notch in Drosophila.

The following Efr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Efr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa03147	NM_132071.3 Latest version!	Drosophila melanogaster ER GDP-fucose transporter (Efr), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa03147	NM_167062.3 Latest version!	Drosophila melanogaster ER GDP-fucose transporter (Efr), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Efr ( NM_167062.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727071.1 Drosophila melanogaster ER GDP-fucose transporter (Efr), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03147
Clone ID Related Accession (Same CDS sequence)	NM_167062.3 , NM_132071.3
Accession Version	NM_167062.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER GDP-fucose transporter, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167062.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCTGA ACCTGAAGGC CCTGCTGGGC ATGCTGTTCG TGTTCATCGG CTGCTGCAGC 
AACGTGGTCT TTCTGGAGCT GATCATTCAA ATCGATCCGG GAGCGGGTAA CCTGATCACC 
TTCGCCCAAT TCCTGTTCAT CGCGCTGGAG GGTCTGGTCT TCACCTCCAA GTTCTTTACG 
GTGCGACCGA AGATTGCGCT GAAGGACTAC GTCATCCTGG TGGCCCTGTT CTTTGGGGCG 
AACGTTTGCA ATAACTATGC CTTCAACTTT AACATACCCA TGCCGCTGCA CATGATCTTC 
CGTAGCGGAT CACTGATGGC CAACATGATC ATGGGCATTG TGCTGCTGAA GAAGCGCTAC 
AATCTGCGAC AGTACAGCTC CGTGGCCATG ATCACGGCTG GCATTATATT GTGCACTCTG 
GTCTCCAGTG GCGATGTCAA GGATAATACA CATCACAGCC TCAAGGTGGA TACATCCTAC 
TCCGATTTCT TCTGGTGGAC GGTGGGTATT GGCTTGCTGA CAATCGCCCT GCTGGTCACC 
GCCTACATGG GCATCTACCA GGAGGTGATA TACAAGAAAT ACGGCAAGCA TCCCAGCGAG 
GCCTTATTCT TTACGCACAT GTTACCTTTG CCCGGTTTTC TGATCATGGC CGGCAATATA 
GTCCAACACT TTGGTATCGC ATGGTCATCG GAACCAGTGG CCGTGCCACT GCTGGGAGCC 
ATTGGACTGG AGTGGAAATT CCCGCTGATG TTGTTCTATC TGCTGTGCAA CGTGGTCACC 
CAGTATGTAT GCATCAGTGC CGTCTACGTT CTGACCACCG AATGTGCATC GCTAACCGTA 
ACGCTGGTGG TCACCCTGCG CAAGTTCGTC TCGCTGCTGT TCTCGATCAT TTATTTCCGG 
AATCCATTCA CCCTAAACCA TTGGGTAGGC ACCATCCTGG TATTCTTCGG CACAATACTC 
TTCGCCAATG TGATCAACCA GGTGAGGGAC GCCTATCGGG CCAGGTCGAG CCGGAAGACC 
CACTTCGACA CCGCTCCGCT GGCCAAGAAG GTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727071.1
CDS	261..1319
Translation	MALNLKALLGMLFVFIGCCSNVVFLELIIQIDPGAGNLITFAQFLFIALEGLVFTSKFFTVRPKIALKDYVILVALFFGANVCNNYAFNFNIPMPLHMIFRSGSLMANMIMGIVLLKKRYNLRQYSSVAMITAGIILCTLVSSGDVKDNTHHSLKVDTSYSDFFWWTVGIGLLTIALLVTAYMGIYQEVIYKKYGKHPSEALFFTHMLPLPGFLIMAGNIVQHFGIAWSSEPVAVPLLGAIGLEWKFPLMLFYLLCNVVTQYVCISAVYVLTTECASLTVTLVVTLRKFVSLLFSIIYFRNPFTLNHWVGTILVFFGTILFANVINQVRDAYRARSSRKTHFDTAPLAKKVE

Target ORF information:

RefSeq Version	NM_167062.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER GDP-fucose transporter (Efr), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167062.3

ORF Insert Sequence:

ATGGCTCTGA ACCTGAAGGC CCTGCTGGGC ATGCTGTTCG TGTTCATCGG CTGCTGCAGC 
AACGTGGTCT TTCTGGAGCT GATCATTCAA ATCGATCCGG GAGCGGGTAA CCTGATCACC 
TTCGCCCAAT TCCTGTTCAT CGCGCTGGAG GGTCTGGTCT TCACCTCCAA GTTCTTTACG 
GTGCGACCGA AGATTGCGCT GAAGGACTAC GTCATCCTGG TGGCCCTGTT CTTTGGGGCG 
AACGTTTGCA ATAACTATGC CTTCAACTTT AACATACCCA TGCCGCTGCA CATGATCTTC 
CGTAGCGGAT CACTGATGGC CAACATGATC ATGGGCATTG TGCTGCTGAA GAAGCGCTAC 
AATCTGCGAC AGTACAGCTC CGTGGCCATG ATCACGGCTG GCATTATATT GTGCACTCTG 
GTCTCCAGTG GCGATGTCAA GGATAATACA CATCACAGCC TCAAGGTGGA TACATCCTAC 
TCCGATTTCT TCTGGTGGAC GGTGGGTATT GGCTTGCTGA CAATCGCCCT GCTGGTCACC 
GCCTACATGG GCATCTACCA GGAGGTGATA TACAAGAAAT ACGGCAAGCA TCCCAGCGAG 
GCCTTATTCT TTACGCACAT GTTACCTTTG CCCGGTTTTC TGATCATGGC CGGCAATATA 
GTCCAACACT TTGGTATCGC ATGGTCATCG GAACCAGTGG CCGTGCCACT GCTGGGAGCC 
ATTGGACTGG AGTGGAAATT CCCGCTGATG TTGTTCTATC TGCTGTGCAA CGTGGTCACC 
CAGTATGTAT GCATCAGTGC CGTCTACGTT CTGACCACCG AATGTGCATC GCTAACCGTA 
ACGCTGGTGG TCACCCTGCG CAAGTTCGTC TCGCTGCTGT TCTCGATCAT TTATTTCCGG 
AATCCATTCA CCCTAAACCA TTGGGTAGGC ACCATCCTGG TATTCTTCGG CACAATACTC 
TTCGCCAATG TGATCAACCA GGTGAGGGAC GCCTATCGGG CCAGGTCGAG CCGGAAGACC 
CACTTCGACA CCGCTCCGCT GGCCAAGAAG GTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Efr ( NM_132071.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572299.1 Drosophila melanogaster ER GDP-fucose transporter (Efr), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03147
Clone ID Related Accession (Same CDS sequence)	NM_167062.3 , NM_132071.3
Accession Version	NM_132071.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ER GDP-fucose transporter, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132071.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCTGA ACCTGAAGGC CCTGCTGGGC ATGCTGTTCG TGTTCATCGG CTGCTGCAGC 
AACGTGGTCT TTCTGGAGCT GATCATTCAA ATCGATCCGG GAGCGGGTAA CCTGATCACC 
TTCGCCCAAT TCCTGTTCAT CGCGCTGGAG GGTCTGGTCT TCACCTCCAA GTTCTTTACG 
GTGCGACCGA AGATTGCGCT GAAGGACTAC GTCATCCTGG TGGCCCTGTT CTTTGGGGCG 
AACGTTTGCA ATAACTATGC CTTCAACTTT AACATACCCA TGCCGCTGCA CATGATCTTC 
CGTAGCGGAT CACTGATGGC CAACATGATC ATGGGCATTG TGCTGCTGAA GAAGCGCTAC 
AATCTGCGAC AGTACAGCTC CGTGGCCATG ATCACGGCTG GCATTATATT GTGCACTCTG 
GTCTCCAGTG GCGATGTCAA GGATAATACA CATCACAGCC TCAAGGTGGA TACATCCTAC 
TCCGATTTCT TCTGGTGGAC GGTGGGTATT GGCTTGCTGA CAATCGCCCT GCTGGTCACC 
GCCTACATGG GCATCTACCA GGAGGTGATA TACAAGAAAT ACGGCAAGCA TCCCAGCGAG 
GCCTTATTCT TTACGCACAT GTTACCTTTG CCCGGTTTTC TGATCATGGC CGGCAATATA 
GTCCAACACT TTGGTATCGC ATGGTCATCG GAACCAGTGG CCGTGCCACT GCTGGGAGCC 
ATTGGACTGG AGTGGAAATT CCCGCTGATG TTGTTCTATC TGCTGTGCAA CGTGGTCACC 
CAGTATGTAT GCATCAGTGC CGTCTACGTT CTGACCACCG AATGTGCATC GCTAACCGTA 
ACGCTGGTGG TCACCCTGCG CAAGTTCGTC TCGCTGCTGT TCTCGATCAT TTATTTCCGG 
AATCCATTCA CCCTAAACCA TTGGGTAGGC ACCATCCTGG TATTCTTCGG CACAATACTC 
TTCGCCAATG TGATCAACCA GGTGAGGGAC GCCTATCGGG CCAGGTCGAG CCGGAAGACC 
CACTTCGACA CCGCTCCGCT GGCCAAGAAG GTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572299.1
CDS	464..1522
Translation	MALNLKALLGMLFVFIGCCSNVVFLELIIQIDPGAGNLITFAQFLFIALEGLVFTSKFFTVRPKIALKDYVILVALFFGANVCNNYAFNFNIPMPLHMIFRSGSLMANMIMGIVLLKKRYNLRQYSSVAMITAGIILCTLVSSGDVKDNTHHSLKVDTSYSDFFWWTVGIGLLTIALLVTAYMGIYQEVIYKKYGKHPSEALFFTHMLPLPGFLIMAGNIVQHFGIAWSSEPVAVPLLGAIGLEWKFPLMLFYLLCNVVTQYVCISAVYVLTTECASLTVTLVVTLRKFVSLLFSIIYFRNPFTLNHWVGTILVFFGTILFANVINQVRDAYRARSSRKTHFDTAPLAKKVE

Target ORF information:

RefSeq Version	NM_132071.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ER GDP-fucose transporter (Efr), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132071.3

ORF Insert Sequence:

ATGGCTCTGA ACCTGAAGGC CCTGCTGGGC ATGCTGTTCG TGTTCATCGG CTGCTGCAGC 
AACGTGGTCT TTCTGGAGCT GATCATTCAA ATCGATCCGG GAGCGGGTAA CCTGATCACC 
TTCGCCCAAT TCCTGTTCAT CGCGCTGGAG GGTCTGGTCT TCACCTCCAA GTTCTTTACG 
GTGCGACCGA AGATTGCGCT GAAGGACTAC GTCATCCTGG TGGCCCTGTT CTTTGGGGCG 
AACGTTTGCA ATAACTATGC CTTCAACTTT AACATACCCA TGCCGCTGCA CATGATCTTC 
CGTAGCGGAT CACTGATGGC CAACATGATC ATGGGCATTG TGCTGCTGAA GAAGCGCTAC 
AATCTGCGAC AGTACAGCTC CGTGGCCATG ATCACGGCTG GCATTATATT GTGCACTCTG 
GTCTCCAGTG GCGATGTCAA GGATAATACA CATCACAGCC TCAAGGTGGA TACATCCTAC 
TCCGATTTCT TCTGGTGGAC GGTGGGTATT GGCTTGCTGA CAATCGCCCT GCTGGTCACC 
GCCTACATGG GCATCTACCA GGAGGTGATA TACAAGAAAT ACGGCAAGCA TCCCAGCGAG 
GCCTTATTCT TTACGCACAT GTTACCTTTG CCCGGTTTTC TGATCATGGC CGGCAATATA 
GTCCAACACT TTGGTATCGC ATGGTCATCG GAACCAGTGG CCGTGCCACT GCTGGGAGCC 
ATTGGACTGG AGTGGAAATT CCCGCTGATG TTGTTCTATC TGCTGTGCAA CGTGGTCACC 
CAGTATGTAT GCATCAGTGC CGTCTACGTT CTGACCACCG AATGTGCATC GCTAACCGTA 
ACGCTGGTGG TCACCCTGCG CAAGTTCGTC TCGCTGCTGT TCTCGATCAT TTATTTCCGG 
AATCCATTCA CCCTAAACCA TTGGGTAGGC ACCATCCTGG TATTCTTCGG CACAATACTC 
TTCGCCAATG TGATCAACCA GGTGAGGGAC GCCTATCGGG CCAGGTCGAG CCGGAAGACC 
CACTTCGACA CCGCTCCGCT GGCCAAGAAG GTGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Efr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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