dhd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dhd
Entrez Gene ID	31444
Full Name	deadhead
Synonyms	4193,CG4193,Dhd,DmTrx-1,Dmdhd,Dmel\CG4193,Dmeldhd,Trx-1
General protein information	Preferred Names deadhead Names CG4193 gene product from transcript CG4193-RB CG4193-PA CG4193-PB dhd-PA dhd-PB thioredoxin thioredoxin-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4F4-4F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297953.1	NP_001284882.1	deadhead, isoform B
NM_078491.3	NP_511046.1	deadhead, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

thioredoxin DHD plays a critical role in early development to facilitate the switch from protamine-based sperm chromatin structures to the somatic nucleosomal chromatin.
Title: Thioredoxin-dependent disulfide bond reduction is required for protamine eviction from sperm chromatin.

Drosophila thioredoxin can function as an anti-aging agent and as a suppressor of Parkin-associated endothelin receptor-like receptor - and poly-glutamine-induced neurotoxicity
Title: Thioredoxin suppresses Parkin-associated endothelin receptor-like receptor-induced neurotoxicity and extends longevity in Drosophila.

dimer dissociation constants K(d) were found to be 2.2mM for reduced DmTrx and above 10mM for oxidized DmTrx as well as for the protein in the presence of reduced glutathione
Title: Comparative structural analysis of oxidized and reduced thioredoxin from Drosophila melanogaster.

evidence for distinct biological function of Trx-1
Title: Thioredoxin-2 but not thioredoxin-1 is a substrate of thioredoxin peroxidase-1 from Drosophila melanogaster: isolation and characterization of a second thioredoxin in D. Melanogaster and evidence for distinct biological functions of Trx-1 and Trx-2.

The following dhd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dhd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00839	NM_001297953.1 Latest version!	Drosophila melanogaster deadhead (dhd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa00839	NM_078491.3 Latest version!	Drosophila melanogaster deadhead (dhd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dhd ( NM_001297953.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284882.1 Drosophila melanogaster deadhead (dhd), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00839
Clone ID Related Accession (Same CDS sequence)	NM_001297953.1 , NM_078491.3
Accession Version	NM_001297953.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deadhead, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCCG TACGCACCAT GAACGACTAT CACAAGCGCA TCGAGGCGGC CGACGACAAG 
CTAATCGTGC TGGATTTCTA TGCGACATGG TGTGGTCCCT GCAAGGAAAT GGAGAGCACC 
GTCAAATCGC TGGCCAGAAA ATACTCCAGC AAGGCGGTGG TGCTCAAGAT CGATGTGGAC 
AAATTCGAGG AGCTGACGGA GCGCTACAAG GTGCGCAGCA TGCCAACGTT TGTCTTTTTG 
CGCCAAAATC GACGCTTGGC CTCCTTTGCC GGCGCCGACG AGCACAAGCT GACCAACATG 
ATGGCCAAGC TGGTGAAGGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284882.1
CDS	290..613
Translation	MASVRTMNDYHKRIEAADDKLIVLDFYATWCGPCKEMESTVKSLARKYSSKAVVLKIDVDKFEELTERYKVRSMPTFVFLRQNRRLASFAGADEHKLTNMMAKLVKA

Target ORF information:

RefSeq Version	NM_001297953.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deadhead (dhd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297953.1

ORF Insert Sequence:

ATGGCATCCG TACGCACCAT GAACGACTAT CACAAGCGCA TCGAGGCGGC CGACGACAAG 
CTAATCGTGC TGGATTTCTA TGCGACATGG TGTGGTCCCT GCAAGGAAAT GGAGAGCACC 
GTCAAATCGC TGGCCAGAAA ATACTCCAGC AAGGCGGTGG TGCTCAAGAT CGATGTGGAC 
AAATTCGAGG AGCTGACGGA GCGCTACAAG GTGCGCAGCA TGCCAACGTT TGTCTTTTTG 
CGCCAAAATC GACGCTTGGC CTCCTTTGCC GGCGCCGACG AGCACAAGCT GACCAACATG 
ATGGCCAAGC TGGTGAAGGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dhd ( NM_078491.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511046.1 Drosophila melanogaster deadhead (dhd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00839
Clone ID Related Accession (Same CDS sequence)	NM_001297953.1 , NM_078491.3
Accession Version	NM_078491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deadhead, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078491.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATCCG TACGCACCAT GAACGACTAT CACAAGCGCA TCGAGGCGGC CGACGACAAG 
CTAATCGTGC TGGATTTCTA TGCGACATGG TGTGGTCCCT GCAAGGAAAT GGAGAGCACC 
GTCAAATCGC TGGCCAGAAA ATACTCCAGC AAGGCGGTGG TGCTCAAGAT CGATGTGGAC 
AAATTCGAGG AGCTGACGGA GCGCTACAAG GTGCGCAGCA TGCCAACGTT TGTCTTTTTG 
CGCCAAAATC GACGCTTGGC CTCCTTTGCC GGCGCCGACG AGCACAAGCT GACCAACATG 
ATGGCCAAGC TGGTGAAGGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511046.1
CDS	107..430
Translation	MASVRTMNDYHKRIEAADDKLIVLDFYATWCGPCKEMESTVKSLARKYSSKAVVLKIDVDKFEELTERYKVRSMPTFVFLRQNRRLASFAGADEHKLTNMMAKLVKA

Target ORF information:

RefSeq Version	NM_078491.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deadhead (dhd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078491.3

ORF Insert Sequence:

ATGGCATCCG TACGCACCAT GAACGACTAT CACAAGCGCA TCGAGGCGGC CGACGACAAG 
CTAATCGTGC TGGATTTCTA TGCGACATGG TGTGGTCCCT GCAAGGAAAT GGAGAGCACC 
GTCAAATCGC TGGCCAGAAA ATACTCCAGC AAGGCGGTGG TGCTCAAGAT CGATGTGGAC 
AAATTCGAGG AGCTGACGGA GCGCTACAAG GTGCGCAGCA TGCCAACGTT TGTCTTTTTG 
CGCCAAAATC GACGCTTGGC CTCCTTTGCC GGCGCCGACG AGCACAAGCT GACCAACATG 
ATGGCCAAGC TGGTGAAGGC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dhd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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