Pdha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdha
Entrez Gene ID	31406
Full Name	lethal (1) G0334
Synonyms	CG 7010,CG7010,Dmel\CG7010,E1-PDH,L(1)G0334,L(1)g0334,PDH,PDHE1,Pdh,l (1) G0334,pdha
General protein information	Preferred Names lethal (1) G0334 Names CG7010 gene product from transcript CG7010-RD CG7010-PA CG7010-PB CG7010-PC CG7010-PD PDH complex l(1)G0334-PA l(1)G0334-PB l(1)G0334-PC l(1)G0334-PD pyruvate dehydrogenase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4C14-4C14

mRNA and Protein(s)

mRNA	Protein	Name
NM_167017.3	NP_726945.1	pyruvate dehydrogenase E1 alpha subunit, isoform C
NM_167018.2	NP_726946.1	pyruvate dehydrogenase E1 alpha subunit, isoform D
NM_167019.2	NP_726947.1	pyruvate dehydrogenase E1 alpha subunit, isoform B
NM_131953.5	NP_572181.4	pyruvate dehydrogenase E1 alpha subunit, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDHA2	NP_005381.1
Pan troglodytes (chimpanzee)	PDHA2	XP_526637.2
Bos taurus (cattle)	PDHA2	NP_001070539.1
	PDHA2	XP_001097880.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003030	XP_311846.5
Rattus norvegicus (Norway rat)	Pdha1l1	XP_006253546.1
Drosophila melanogaster (fruit fly)	l(1)G0334	NP_572181.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pdha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00800	NM_131953.5 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant A (Pdha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00800	NM_167018.2 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant D (Pdha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa00801	NM_167019.2 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant B (Pdha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00802	NM_167017.3 Latest version!	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant C (Pdha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdha ( NM_167018.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726946.1 Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant D (Pdha), mRNA.

Price & Availability↑

CloneID	OFa00800
Clone ID Related Accession (Same CDS sequence)	NM_167018.2 , NM_131953.5
Accession Version	NM_167018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 alpha subunit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_167018.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGTA CTCTGTCACG CGTTAGCGAA TTGCCCATTA TTGTTAAGCA ACTGCAAAAA 
AATGCAGCCC AAGCAGGTGT TTCGAAAACC AACAATTATG CCACGGAGGC CACCGTTCAA 
GTGAATCGGC CCTTCAAGCT GCACCGCCTG GACGAAGGAC CCGCCACGGA AGTGAAGCTG 
ACCAAGGATC AGGCCCTCAA GTACTACACA CAGATGCAGA CGATCCGTCG GTTGGAGACG 
GCCGCCGGTA ATCTGTACAA GGAGAAGATC ATTCGCGGCT TCTGTCATCT GTATTCCGGC 
CAGGAGGCAT GCGCCGTTGG CATGAAGGCG GCGATGCGGG ATGTGGACAA CATCATCTCG 
GCGTACCGTG TCCACGGATG GACCTACCTG ATGGGCGTCT CACCCAGCGG CGTACTGGCT 
GAGCTCACCG GTGTCCAGGG TGGCTGTGCC CGCGGCAAGG GTGGCTCCAT GCACATGTAC 
GCACCGAACT TCTACGGAGG CAATGGCATC GTGGGTGCTC AAGTTCCTTT GGGTGCCGGC 
GTTGGCTTGG CCTGCAAGTA CAAGGGCAAT GGCGGCATGT GCCTGGCCCT CTACGGTGAT 
GGTGCCGCCA ATCAAGGACA GGTGTTCGAG GCTTACAACA TGGCCTACCT GTGGAAACTG 
CCTGTGATCT TTGTCTGCGA GAACAACAAC TACGGAATGG GCACCAGTTC TGAGCGTGCT 
TCCTGCAACA CTGATTACTA TACCCGCGGC GACGCTCTGC CCGGAATCTG GGTGGACGGC 
ATGGATGTGC TGGCCGTGCG CAGCGCCACC GAGTTCGCCA TCAACTATGT GAACACTCAC 
GGACCTTTGG TGATGGAGAC GAACACGTAC CGATACTCTG GACACTCCAT GTCCGATCCG 
GGCACATCGT ACCGCACACG CGAGGAGATC CAGGAGGTGC GCCAGAAGCG TGATCCCATC 
ACCTCCTTCA AGGAGCTGTG CATCGAGCTG GGTCTGATTA CCACCGATGA AGTTAAGGCC 
ATCGATCTGA AGGTACGCAA GGAGGTCGAC GAGGCCACCG CCTTCGCCAA GTCCGATGCC 
GAGCTGGGCG TGAGCCACCT CTGGACGGAC GTGTACTCCA ACAATCTGGA GCCCAAGCTG 
CGCGGCACCA TCGCCTACGA TATTGATCAC ATCCAGGAGC GCAAGGGCGT CAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726946.1
CDS	115..1314
Translation	MLRTLSRVSELPIIVKQLQKNAAQAGVSKTNNYATEATVQVNRPFKLHRLDEGPATEVKLTKDQALKYYTQMQTIRRLETAAGNLYKEKIIRGFCHLYSGQEACAVGMKAAMRDVDNIISAYRVHGWTYLMGVSPSGVLAELTGVQGGCARGKGGSMHMYAPNFYGGNGIVGAQVPLGAGVGLACKYKGNGGMCLALYGDGAANQGQVFEAYNMAYLWKLPVIFVCENNNYGMGTSSERASCNTDYYTRGDALPGIWVDGMDVLAVRSATEFAINYVNTHGPLVMETNTYRYSGHSMSDPGTSYRTREEIQEVRQKRDPITSFKELCIELGLITTDEVKAIDLKVRKEVDEATAFAKSDAELGVSHLWTDVYSNNLEPKLRGTIAYDIDHIQERKGVNH

Target ORF information:

RefSeq Version	NM_167018.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant D (Pdha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167018.2

ORF Insert Sequence:

ATGTTGCGTA CTCTGTCACG CGTTAGCGAA TTGCCCATTA TTGTTAAGCA ACTGCAAAAA 
AATGCAGCCC AAGCAGGTGT TTCGAAAACC AACAATTATG CCACGGAGGC CACCGTTCAA 
GTGAATCGGC CCTTCAAGCT GCACCGCCTG GACGAAGGAC CCGCCACGGA AGTGAAGCTG 
ACCAAGGATC AGGCCCTCAA GTACTACACA CAGATGCAGA CGATCCGTCG GTTGGAGACG 
GCCGCCGGTA ATCTGTACAA GGAGAAGATC ATTCGCGGCT TCTGTCATCT GTATTCCGGC 
CAGGAGGCAT GCGCCGTTGG CATGAAGGCG GCGATGCGGG ATGTGGACAA CATCATCTCG 
GCGTACCGTG TCCACGGATG GACCTACCTG ATGGGCGTCT CACCCAGCGG CGTACTGGCT 
GAGCTCACCG GTGTCCAGGG TGGCTGTGCC CGCGGCAAGG GTGGCTCCAT GCACATGTAC 
GCACCGAACT TCTACGGAGG CAATGGCATC GTGGGTGCTC AAGTTCCTTT GGGTGCCGGC 
GTTGGCTTGG CCTGCAAGTA CAAGGGCAAT GGCGGCATGT GCCTGGCCCT CTACGGTGAT 
GGTGCCGCCA ATCAAGGACA GGTGTTCGAG GCTTACAACA TGGCCTACCT GTGGAAACTG 
CCTGTGATCT TTGTCTGCGA GAACAACAAC TACGGAATGG GCACCAGTTC TGAGCGTGCT 
TCCTGCAACA CTGATTACTA TACCCGCGGC GACGCTCTGC CCGGAATCTG GGTGGACGGC 
ATGGATGTGC TGGCCGTGCG CAGCGCCACC GAGTTCGCCA TCAACTATGT GAACACTCAC 
GGACCTTTGG TGATGGAGAC GAACACGTAC CGATACTCTG GACACTCCAT GTCCGATCCG 
GGCACATCGT ACCGCACACG CGAGGAGATC CAGGAGGTGC GCCAGAAGCG TGATCCCATC 
ACCTCCTTCA AGGAGCTGTG CATCGAGCTG GGTCTGATTA CCACCGATGA AGTTAAGGCC 
ATCGATCTGA AGGTACGCAA GGAGGTCGAC GAGGCCACCG CCTTCGCCAA GTCCGATGCC 
GAGCTGGGCG TGAGCCACCT CTGGACGGAC GTGTACTCCA ACAATCTGGA GCCCAAGCTG 
CGCGGCACCA TCGCCTACGA TATTGATCAC ATCCAGGAGC GCAAGGGCGT CAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdha ( NM_131953.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572181.4 Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant A (Pdha), mRNA.

Price & Availability↑

CloneID	OFa00800
Clone ID Related Accession (Same CDS sequence)	NM_167018.2 , NM_131953.5
Accession Version	NM_131953.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 alpha subunit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_131953.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGTA CTCTGTCACG CGTTAGCGAA TTGCCCATTA TTGTTAAGCA ACTGCAAAAA 
AATGCAGCCC AAGCAGGTGT TTCGAAAACC AACAATTATG CCACGGAGGC CACCGTTCAA 
GTGAATCGGC CCTTCAAGCT GCACCGCCTG GACGAAGGAC CCGCCACGGA AGTGAAGCTG 
ACCAAGGATC AGGCCCTCAA GTACTACACA CAGATGCAGA CGATCCGTCG GTTGGAGACG 
GCCGCCGGTA ATCTGTACAA GGAGAAGATC ATTCGCGGCT TCTGTCATCT GTATTCCGGC 
CAGGAGGCAT GCGCCGTTGG CATGAAGGCG GCGATGCGGG ATGTGGACAA CATCATCTCG 
GCGTACCGTG TCCACGGATG GACCTACCTG ATGGGCGTCT CACCCAGCGG CGTACTGGCT 
GAGCTCACCG GTGTCCAGGG TGGCTGTGCC CGCGGCAAGG GTGGCTCCAT GCACATGTAC 
GCACCGAACT TCTACGGAGG CAATGGCATC GTGGGTGCTC AAGTTCCTTT GGGTGCCGGC 
GTTGGCTTGG CCTGCAAGTA CAAGGGCAAT GGCGGCATGT GCCTGGCCCT CTACGGTGAT 
GGTGCCGCCA ATCAAGGACA GGTGTTCGAG GCTTACAACA TGGCCTACCT GTGGAAACTG 
CCTGTGATCT TTGTCTGCGA GAACAACAAC TACGGAATGG GCACCAGTTC TGAGCGTGCT 
TCCTGCAACA CTGATTACTA TACCCGCGGC GACGCTCTGC CCGGAATCTG GGTGGACGGC 
ATGGATGTGC TGGCCGTGCG CAGCGCCACC GAGTTCGCCA TCAACTATGT GAACACTCAC 
GGACCTTTGG TGATGGAGAC GAACACGTAC CGATACTCTG GACACTCCAT GTCCGATCCG 
GGCACATCGT ACCGCACACG CGAGGAGATC CAGGAGGTGC GCCAGAAGCG TGATCCCATC 
ACCTCCTTCA AGGAGCTGTG CATCGAGCTG GGTCTGATTA CCACCGATGA AGTTAAGGCC 
ATCGATCTGA AGGTACGCAA GGAGGTCGAC GAGGCCACCG CCTTCGCCAA GTCCGATGCC 
GAGCTGGGCG TGAGCCACCT CTGGACGGAC GTGTACTCCA ACAATCTGGA GCCCAAGCTG 
CGCGGCACCA TCGCCTACGA TATTGATCAC ATCCAGGAGC GCAAGGGCGT CAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572181.4
CDS	208..1407
Translation	MLRTLSRVSELPIIVKQLQKNAAQAGVSKTNNYATEATVQVNRPFKLHRLDEGPATEVKLTKDQALKYYTQMQTIRRLETAAGNLYKEKIIRGFCHLYSGQEACAVGMKAAMRDVDNIISAYRVHGWTYLMGVSPSGVLAELTGVQGGCARGKGGSMHMYAPNFYGGNGIVGAQVPLGAGVGLACKYKGNGGMCLALYGDGAANQGQVFEAYNMAYLWKLPVIFVCENNNYGMGTSSERASCNTDYYTRGDALPGIWVDGMDVLAVRSATEFAINYVNTHGPLVMETNTYRYSGHSMSDPGTSYRTREEIQEVRQKRDPITSFKELCIELGLITTDEVKAIDLKVRKEVDEATAFAKSDAELGVSHLWTDVYSNNLEPKLRGTIAYDIDHIQERKGVNH

Target ORF information:

RefSeq Version	NM_131953.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant A (Pdha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131953.5

ORF Insert Sequence:

ATGTTGCGTA CTCTGTCACG CGTTAGCGAA TTGCCCATTA TTGTTAAGCA ACTGCAAAAA 
AATGCAGCCC AAGCAGGTGT TTCGAAAACC AACAATTATG CCACGGAGGC CACCGTTCAA 
GTGAATCGGC CCTTCAAGCT GCACCGCCTG GACGAAGGAC CCGCCACGGA AGTGAAGCTG 
ACCAAGGATC AGGCCCTCAA GTACTACACA CAGATGCAGA CGATCCGTCG GTTGGAGACG 
GCCGCCGGTA ATCTGTACAA GGAGAAGATC ATTCGCGGCT TCTGTCATCT GTATTCCGGC 
CAGGAGGCAT GCGCCGTTGG CATGAAGGCG GCGATGCGGG ATGTGGACAA CATCATCTCG 
GCGTACCGTG TCCACGGATG GACCTACCTG ATGGGCGTCT CACCCAGCGG CGTACTGGCT 
GAGCTCACCG GTGTCCAGGG TGGCTGTGCC CGCGGCAAGG GTGGCTCCAT GCACATGTAC 
GCACCGAACT TCTACGGAGG CAATGGCATC GTGGGTGCTC AAGTTCCTTT GGGTGCCGGC 
GTTGGCTTGG CCTGCAAGTA CAAGGGCAAT GGCGGCATGT GCCTGGCCCT CTACGGTGAT 
GGTGCCGCCA ATCAAGGACA GGTGTTCGAG GCTTACAACA TGGCCTACCT GTGGAAACTG 
CCTGTGATCT TTGTCTGCGA GAACAACAAC TACGGAATGG GCACCAGTTC TGAGCGTGCT 
TCCTGCAACA CTGATTACTA TACCCGCGGC GACGCTCTGC CCGGAATCTG GGTGGACGGC 
ATGGATGTGC TGGCCGTGCG CAGCGCCACC GAGTTCGCCA TCAACTATGT GAACACTCAC 
GGACCTTTGG TGATGGAGAC GAACACGTAC CGATACTCTG GACACTCCAT GTCCGATCCG 
GGCACATCGT ACCGCACACG CGAGGAGATC CAGGAGGTGC GCCAGAAGCG TGATCCCATC 
ACCTCCTTCA AGGAGCTGTG CATCGAGCTG GGTCTGATTA CCACCGATGA AGTTAAGGCC 
ATCGATCTGA AGGTACGCAA GGAGGTCGAC GAGGCCACCG CCTTCGCCAA GTCCGATGCC 
GAGCTGGGCG TGAGCCACCT CTGGACGGAC GTGTACTCCA ACAATCTGGA GCCCAAGCTG 
CGCGGCACCA TCGCCTACGA TATTGATCAC ATCCAGGAGC GCAAGGGCGT CAATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdha ( NM_167019.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726947.1 Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant B (Pdha), mRNA.

Price & Availability↑

CloneID	OFa00801
Clone ID Related Accession (Same CDS sequence)	NM_167019.2
Accession Version	NM_167019.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 alpha subunit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167019.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGACGA TCCGTCGGTT GGAGACGGCC GCCGGTAATC TGTACAAGGA GAAGATCATT 
CGCGGCTTCT GTCATCTGTA TTCCGGCCAG GAGGCATGCG CCGTTGGCAT GAAGGCGGCG 
ATGCGGGATG TGGACAACAT CATCTCGGCG TACCGTGTCC ACGGATGGAC CTACCTGATG 
GGCGTCTCAC CCAGCGGCGT ACTGGCTGAG CTCACCGGTG TCCAGGGTGG CTGTGCCCGC 
GGCAAGGGTG GCTCCATGCA CATGTACGCA CCGAACTTCT ACGGAGGCAA TGGCATCGTG 
GGTGCTCAAG TTCCTTTGGG TGCCGGCGTT GGCTTGGCCT GCAAGTACAA GGGCAATGGC 
GGCATGTGCC TGGCCCTCTA CGGTGATGGT GCCGCCAATC AAGGACAGGT GTTCGAGGCT 
TACAACATGG CCTACCTGTG GAAACTGCCT GTGATCTTTG TCTGCGAGAA CAACAACTAC 
GGAATGGGCA CCAGTTCTGA GCGTGCTTCC TGCAACACTG ATTACTATAC CCGCGGCGAC 
GCTCTGCCCG GAATCTGGGT GGACGGCATG GATGTGCTGG CCGTGCGCAG CGCCACCGAG 
TTCGCCATCA ACTATGTGAA CACTCACGGA CCTTTGGTGA TGGAGACGAA CACGTACCGA 
TACTCTGGAC ACTCCATGTC CGATCCGGGC ACATCGTACC GCACACGCGA GGAGATCCAG 
GAGGTGCGCC AGAAGCGTGA TCCCATCACC TCCTTCAAGG AGCTGTGCAT CGAGCTGGGT 
CTGATTACCA CCGATGAAGT TAAGGCCATC GATCTGAAGG TACGCAAGGA GGTCGACGAG 
GCCACCGCCT TCGCCAAGTC CGATGCCGAG CTGGGCGTGA GCCACCTCTG GACGGACGTG 
TACTCCAACA ATCTGGAGCC CAAGCTGCGC GGCACCATCG CCTACGATAT TGATCACATC 
CAGGAGCGCA AGGGCGTCAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726947.1
CDS	653..1639
Translation	MQTIRRLETAAGNLYKEKIIRGFCHLYSGQEACAVGMKAAMRDVDNIISAYRVHGWTYLMGVSPSGVLAELTGVQGGCARGKGGSMHMYAPNFYGGNGIVGAQVPLGAGVGLACKYKGNGGMCLALYGDGAANQGQVFEAYNMAYLWKLPVIFVCENNNYGMGTSSERASCNTDYYTRGDALPGIWVDGMDVLAVRSATEFAINYVNTHGPLVMETNTYRYSGHSMSDPGTSYRTREEIQEVRQKRDPITSFKELCIELGLITTDEVKAIDLKVRKEVDEATAFAKSDAELGVSHLWTDVYSNNLEPKLRGTIAYDIDHIQERKGVNH

Target ORF information:

RefSeq Version	NM_167019.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant B (Pdha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167019.2

ORF Insert Sequence:

ATGCAGACGA TCCGTCGGTT GGAGACGGCC GCCGGTAATC TGTACAAGGA GAAGATCATT 
CGCGGCTTCT GTCATCTGTA TTCCGGCCAG GAGGCATGCG CCGTTGGCAT GAAGGCGGCG 
ATGCGGGATG TGGACAACAT CATCTCGGCG TACCGTGTCC ACGGATGGAC CTACCTGATG 
GGCGTCTCAC CCAGCGGCGT ACTGGCTGAG CTCACCGGTG TCCAGGGTGG CTGTGCCCGC 
GGCAAGGGTG GCTCCATGCA CATGTACGCA CCGAACTTCT ACGGAGGCAA TGGCATCGTG 
GGTGCTCAAG TTCCTTTGGG TGCCGGCGTT GGCTTGGCCT GCAAGTACAA GGGCAATGGC 
GGCATGTGCC TGGCCCTCTA CGGTGATGGT GCCGCCAATC AAGGACAGGT GTTCGAGGCT 
TACAACATGG CCTACCTGTG GAAACTGCCT GTGATCTTTG TCTGCGAGAA CAACAACTAC 
GGAATGGGCA CCAGTTCTGA GCGTGCTTCC TGCAACACTG ATTACTATAC CCGCGGCGAC 
GCTCTGCCCG GAATCTGGGT GGACGGCATG GATGTGCTGG CCGTGCGCAG CGCCACCGAG 
TTCGCCATCA ACTATGTGAA CACTCACGGA CCTTTGGTGA TGGAGACGAA CACGTACCGA 
TACTCTGGAC ACTCCATGTC CGATCCGGGC ACATCGTACC GCACACGCGA GGAGATCCAG 
GAGGTGCGCC AGAAGCGTGA TCCCATCACC TCCTTCAAGG AGCTGTGCAT CGAGCTGGGT 
CTGATTACCA CCGATGAAGT TAAGGCCATC GATCTGAAGG TACGCAAGGA GGTCGACGAG 
GCCACCGCCT TCGCCAAGTC CGATGCCGAG CTGGGCGTGA GCCACCTCTG GACGGACGTG 
TACTCCAACA ATCTGGAGCC CAAGCTGCGC GGCACCATCG CCTACGATAT TGATCACATC 
CAGGAGCGCA AGGGCGTCAA TCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdha ( NM_167017.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726945.1 Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant C (Pdha), mRNA.

Price & Availability↑

CloneID	OFa00802
Clone ID Related Accession (Same CDS sequence)	NM_167017.3
Accession Version	NM_167017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pyruvate dehydrogenase E1 alpha subunit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167017.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCGTA CTCTGTCACG CGTTAGCGAA TTGCCCATTA TTGTTAAGCA ACTGCAAAAA 
CGTTGCCATA ACAACAACAA CAACAACATC ATCATCAGCG GATGTCGGAT ACTTAGCAAC 
AACAACAACA ACAGCAGCAT CGATATCATC AGGAATGGGA GATATTCATC GTCGTTTTTC 
GGTTTATTCC AGAATGCAGC CCAAGCAGGT GTTTCGAAAA CCAACAATTA TGCCACGGAG 
GCCACCGTTC AAGTGAATCG GCCCTTCAAG CTGCACCGCC TGGACGAAGG ACCCGCCACG 
GAAGTGAAGC TGACCAAGGA TCAGGCCCTC AAGTACTACA CACAGATGCA GACGATCCGT 
CGGTTGGAGA CGGCCGCCGG TAATCTGTAC AAGGAGAAGA TCATTCGCGG CTTCTGTCAT 
CTGTATTCCG GCCAGGAGGC ATGCGCCGTT GGCATGAAGG CGGCGATGCG GGATGTGGAC 
AACATCATCT CGGCGTACCG TGTCCACGGA TGGACCTACC TGATGGGCGT CTCACCCAGC 
GGCGTACTGG CTGAGCTCAC CGGTGTCCAG GGTGGCTGTG CCCGCGGCAA GGGTGGCTCC 
ATGCACATGT ACGCACCGAA CTTCTACGGA GGCAATGGCA TCGTGGGTGC TCAAGTTCCT 
TTGGGTGCCG GCGTTGGCTT GGCCTGCAAG TACAAGGGCA ATGGCGGCAT GTGCCTGGCC 
CTCTACGGTG ATGGTGCCGC CAATCAAGGA CAGGTGTTCG AGGCTTACAA CATGGCCTAC 
CTGTGGAAAC TGCCTGTGAT CTTTGTCTGC GAGAACAACA ACTACGGAAT GGGCACCAGT 
TCTGAGCGTG CTTCCTGCAA CACTGATTAC TATACCCGCG GCGACGCTCT GCCCGGAATC 
TGGGTGGACG GCATGGATGT GCTGGCCGTG CGCAGCGCCA CCGAGTTCGC CATCAACTAT 
GTGAACACTC ACGGACCTTT GGTGATGGAG ACGAACACGT ACCGATACTC TGGACACTCC 
ATGTCCGATC CGGGCACATC GTACCGCACA CGCGAGGAGA TCCAGGAGGT GCGCCAGAAG 
CGTGATCCCA TCACCTCCTT CAAGGAGCTG TGCATCGAGC TGGGTCTGAT TACCACCGAT 
GAAGTTAAGG CCATCGATCT GAAGGTACGC AAGGAGGTCG ACGAGGCCAC CGCCTTCGCC 
AAGTCCGATG CCGAGCTGGG CGTGAGCCAC CTCTGGACGG ACGTGTACTC CAACAATCTG 
GAGCCCAAGC TGCGCGGCAC CATCGCCTAC GATATTGATC ACATCCAGGA GCGCAAGGGC 
GTCAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726945.1
CDS	208..1539
Translation	MLRTLSRVSELPIIVKQLQKRCHNNNNNNIIISGCRILSNNNNNSSIDIIRNGRYSSSFFGLFQNAAQAGVSKTNNYATEATVQVNRPFKLHRLDEGPATEVKLTKDQALKYYTQMQTIRRLETAAGNLYKEKIIRGFCHLYSGQEACAVGMKAAMRDVDNIISAYRVHGWTYLMGVSPSGVLAELTGVQGGCARGKGGSMHMYAPNFYGGNGIVGAQVPLGAGVGLACKYKGNGGMCLALYGDGAANQGQVFEAYNMAYLWKLPVIFVCENNNYGMGTSSERASCNTDYYTRGDALPGIWVDGMDVLAVRSATEFAINYVNTHGPLVMETNTYRYSGHSMSDPGTSYRTREEIQEVRQKRDPITSFKELCIELGLITTDEVKAIDLKVRKEVDEATAFAKSDAELGVSHLWTDVYSNNLEPKLRGTIAYDIDHIQERKGVNH

Target ORF information:

RefSeq Version	NM_167017.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pyruvate dehydrogenase E1 alpha subunit, transcript variant C (Pdha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167017.3

ORF Insert Sequence:

ATGTTGCGTA CTCTGTCACG CGTTAGCGAA TTGCCCATTA TTGTTAAGCA ACTGCAAAAA 
CGTTGCCATA ACAACAACAA CAACAACATC ATCATCAGCG GATGTCGGAT ACTTAGCAAC 
AACAACAACA ACAGCAGCAT CGATATCATC AGGAATGGGA GATATTCATC GTCGTTTTTC 
GGTTTATTCC AGAATGCAGC CCAAGCAGGT GTTTCGAAAA CCAACAATTA TGCCACGGAG 
GCCACCGTTC AAGTGAATCG GCCCTTCAAG CTGCACCGCC TGGACGAAGG ACCCGCCACG 
GAAGTGAAGC TGACCAAGGA TCAGGCCCTC AAGTACTACA CACAGATGCA GACGATCCGT 
CGGTTGGAGA CGGCCGCCGG TAATCTGTAC AAGGAGAAGA TCATTCGCGG CTTCTGTCAT 
CTGTATTCCG GCCAGGAGGC ATGCGCCGTT GGCATGAAGG CGGCGATGCG GGATGTGGAC 
AACATCATCT CGGCGTACCG TGTCCACGGA TGGACCTACC TGATGGGCGT CTCACCCAGC 
GGCGTACTGG CTGAGCTCAC CGGTGTCCAG GGTGGCTGTG CCCGCGGCAA GGGTGGCTCC 
ATGCACATGT ACGCACCGAA CTTCTACGGA GGCAATGGCA TCGTGGGTGC TCAAGTTCCT 
TTGGGTGCCG GCGTTGGCTT GGCCTGCAAG TACAAGGGCA ATGGCGGCAT GTGCCTGGCC 
CTCTACGGTG ATGGTGCCGC CAATCAAGGA CAGGTGTTCG AGGCTTACAA CATGGCCTAC 
CTGTGGAAAC TGCCTGTGAT CTTTGTCTGC GAGAACAACA ACTACGGAAT GGGCACCAGT 
TCTGAGCGTG CTTCCTGCAA CACTGATTAC TATACCCGCG GCGACGCTCT GCCCGGAATC 
TGGGTGGACG GCATGGATGT GCTGGCCGTG CGCAGCGCCA CCGAGTTCGC CATCAACTAT 
GTGAACACTC ACGGACCTTT GGTGATGGAG ACGAACACGT ACCGATACTC TGGACACTCC 
ATGTCCGATC CGGGCACATC GTACCGCACA CGCGAGGAGA TCCAGGAGGT GCGCCAGAAG 
CGTGATCCCA TCACCTCCTT CAAGGAGCTG TGCATCGAGC TGGGTCTGAT TACCACCGAT 
GAAGTTAAGG CCATCGATCT GAAGGTACGC AAGGAGGTCG ACGAGGCCAC CGCCTTCGCC 
AAGTCCGATG CCGAGCTGGG CGTGAGCCAC CTCTGGACGG ACGTGTACTC CAACAATCTG 
GAGCCCAAGC TGCGCGGCAC CATCGCCTAC GATATTGATC ACATCCAGGA GCGCAAGGGC 
GTCAATCACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pdha cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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