peb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	peb
Entrez Gene ID	31391
Full Name	pebbled
Synonyms	CG12212,Dmel\CG12212,EG:66A1.1,EP55,HNT,Hin,Hind,Hnt,PEB,Peb,anon-4Cg,hnd,hnt
General protein information	Preferred Names pebbled Names CG12212 gene product from transcript CG12212-RB CG12212-PA CG12212-PB hindsight peb-PA peb-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4C10-4C10\|1-7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057326.5	NP_476674.3	pebbled, isoform A
NM_001014722.2	NP_001014722.1	pebbled, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HNT regulates R-cell axon targeting through transcriptional regulation of multiple genes
Title: Hindsight regulates photoreceptor axon targeting through transcriptional control of jitterbug/Filamin and multiple genes involved in axon guidance in Drosophila.

Thus HNT and c are functional homologs at the level of DNA binding, transcriptional regulation and developmental control
Title: Drosophila Hindsight and mammalian RREB-1 are evolutionarily conserved DNA-binding transcriptional attenuators.

Hindsight functions in the determined R1/R6 precursor cells to allow Delta transcription to reach high enough levels at the right time to induce the cone-cell determinants Prospero and D-Pax2 in neighboring cells
Title: Hindsight modulates Delta expression during Drosophila cone cell induction.

essential to reduce cell-cell adhesion when epithelial cells within an interconnected group undergo dynamic changes in cell shape
Title: Regulation of cell adhesion and collective cell migration by hindsight and its human homolog RREB1.

Our studies suggest that Hindsight bridges the two antagonistic pathways, Notch and Hedgehog, in the temporal regulation of follicle-cell proliferation and differentiation.
Title: Hindsight mediates the role of notch in suppressing hedgehog signaling and cell proliferation.

The following peb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the peb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00787	NM_057326.5 Latest version!	Drosophila melanogaster pebbled (peb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa00787	NM_001014722.2 Latest version!	Drosophila melanogaster pebbled (peb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

peb ( NM_057326.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476674.3 Drosophila melanogaster pebbled (peb), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00787
Clone ID Related Accession (Same CDS sequence)	NM_057326.5 , NM_001014722.2
Accession Version	NM_057326.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebbled, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057326.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCGCCG CACAGCAACA GCACAACAAC AGCACAGTGG AATCGGAAAT GGAGCGCCAG 
CGACGGGACT CCACCACATC GGAGTCCTCG CTGGAGCATC TGGATCTGGG ACGTACGCCC 
AAAAAACTGG GCGGTAACAG TGGCTCTACA CAGACCACAT CCACGCCACA TGAGCTGGCT 
ACGGTGACAT CTTCGCGCAA GCGCAAAATT CGCCATTTGC AATTGAATCA CCATCAGCAG 
CAGCAGCATC ACCAGCAGTC GGATCTGCTG AGTGATGAAG ACGTGGCGGA GGCGGAGGCG 
GAGGAGGATG AGGACGAGGA GGATGGCGAC CAGGTGGCCG CCTTGGGCTC AAGGAATCTG 
GGACGGCACA AGCAGCGTCG CAGTGGTGGC GCCACCACAC AGGCATCCAT TGTGATGGAC 
TACAGCAGTG GCGATGCCAG TTCGCTGCGC AAGAAATTTC GTCTGAATCG CTCGGCGGCC 
AGTCTCTCGG AATCGGGATT CGTGGACGCC AGCAGCACCA CTGGCCACAG TGGCTATCTG 
GGTAACAGTA GCAGCGCTAC GAACACTACT GCGACCAGTG GTATTGGTGC TAGCGCAGTG 
GCTCCATCTC CAGTCGGCGG AGCAGCGATC AACGCAGCAT CCAGCAGCAG CGGCAGCAGC 
AGTGGCGGAT CCGGTGGCTC CTCGCCCCAG GGACAGTGCC TCTCCAGCGG TGAATCAGGC 
ATCGGAGCCG GCGACGAGCA TATGAAATAC CTTTGCCCAA TCTGTGAGGT GGTCTCAGCC 
ACGCCGCACG AGTTCACCAA CCACATCCGG TGCCACAATT ACGCCAATGG CGATACGGAG 
AACTTCACCT GCCGCATTTG CTCCAAGGTG CTATCCTCGG CTTCATCCCT GGACAGACAC 
GTGCTGGTTC ACACGGGCGA GCGTCCCTTT AACTGCCGCT ACTGCCACCT GACGTTCACC 
ACCAATGGCA ACATGCACCG TCACATGCGC ACCCACAAAC AGCATCAAGT TGCGCAGTCG 
CAGTCGCAGT CGCAGCAGCA GCAGTCGCTG CAGCAGCAGC AACAGTCGCA GCAGCAGCGC 
CGTCAGCAGC AGCAACATCA GCCGTCGCAG CAGCAGCAGA ATCCCGCCCA GCAGCAATTG 
ATGGGCAACA CGTTATCCGC CCGAGCGGAG AGCTACGAAA GCGATGCCAG CTGCTCCACG 
GACGTGTCCA GTGGACATAG TCACAGTCGC AGCAGCAGTT CCCTTAACAA CAACAACAAC 
AACTCGCACA AGGCTAACAA CAATCTAAAG GACTTGGAGG AGCTGGAGGT TTCGACTGAG 
GATCAGGATA CGGAGAACAA GCAGCGTCGA CTGAAGACCA CCATCAACAA CAACATCATC 
GAGAGTGAGC AGCAGGAGGA TATGGATGAT GAGGAGGCCG ACGATGCGGA TGTGGCCATG 
CTGACCAGCA CTCCCGATGT CGCCACTCTT TTGGCCGGAG CCAGTGCATC TGGCGCCGCC 
TCGCGTTCCC CTACCCCATC GCCATCCGCC TCACCTGCCT TGCTCCTAAG TTGTCCCGCC 
TGCGGAGCCT CCGATTTCGA GACCCTGCCC GCTCTCTGCG TCCATTTGGA TGCAATGCAC 
TCAGACATAC CAGCCAAGTG TCGGGACTGC GAGGTGATCT TTGCCACCCA TCGTCAGTTG 
CAGTCGCACT GTTGTCGCTT ACCCAACGCT CTGGCCGGTG GCTTACCGCC GCTTCTCGGA 
GCCAGCAGTT CGCCATTGCA CAACGAGGAG CCGGAGGATG AGGAGCACGG TGACGATGAG 
GATCTCGAGC AGAAGGAGAG GTTAGCGAGT CAAAGCGAGG ACTTTTTCCA TCAGTTGTAT 
CTGAAGCACA AGACAGCAAA TGGATGCGGA GCCATCTCGC ATCCGCCATC GCCCATCAAA 
CATGAGCCGG CGGACACCAA GGATCTGGCC GATATCCAGA GCATCCTGAA CATGACCAGC 
TCCAGTTCCA GTTTTCTGCG CAACTTTGAG CAGTCCGTGA ACACGCCCAA TTCGAGTCAG 
TACAGCCTGG ACGGTCGGGA TCAGGAGGAG GAGGCACAGG ACGCGTTCAC CTCGGAGTTC 
CGTCGCATGA AGCTGCGCGG CGAGTTTCCC TGCAAGCTGT GCACGGCCGT TTTCCCGAAC 
CTTCGTGCCC TCAAAGGTCA CAATCGGGTA CATTTGGGTG CAGTGGGTCC AGCCGGACCC 
TTTCGCTGCA ACATGTGTCC CTACGCCGTT TGCGACAAGG CCGCCCTGGT GCGTCACATG 
CGTACCCACA ACGGAGATCG TCCGTACGAG TGCGCCGTGT GCAACTATGC CTTCACCACC 
AAGGCGAACT GTGAACGTCA TCTGAGGAAT CGCCATGGGA AGACCAGTCG CGAGGAGGTG 
AAGCGAGCCA TTGTCTACCA TCCGGCGGAG GATGCTGGCT GTGAGGACAG CAAATCGCGT 
CTGGGTGAAG ATCTGGCTGA TACGAGTTTC CGTTCGATCA GTCCCACGCC ACCGCCTCCT 
CCGGTAAATG AGAGCAAATC CCAGCTGAAG CACATGCTTC TCGGCGAGAA TCACCTGGCT 
CCTGTTAACC AACAGCCGCC GCTGAAGATC CAGGTGAAGA GCCTCGACCA ACTAGTGGAC 
AAGAAGCCAT CGGCACCAGC TCCCCAGCAG CAACAGCAGC AGCAGCAGCA GGAGAAGAGC 
GGATCTGCCC TAGACTTCAG CATGGATGTT TTGGATCTCA GCAAGAAGCC AACTGGCGGA 
GCATCGCTGA CACCGGCGGT CACTCGCACA CCGACGCCCG CTGCAGTTGC TCCAGTGACG 
CCTGGCGGCG TTGGAACTCC TGACCTGGCC GCCGCCATTG AGCAGCAACA GCTGCTACTG 
GCCCAGCAGC AACTCTTTGG TGCTGGCGGC GAGTATATGC AGCAGCTATT CCGCAGTCTC 
ATGTTCCAGT CCCAGACGTC GGGATTCCCG TTCTTTCCCT TTATGGCACC ACCACCGCCA 
CAAGCAAATC CGGAGAAGCC ACCGATGGTG AGTCCACCAA ACCGTATCAA TCCCATGCCC 
GTGGGCGTTG GAGTTGGCGT GCCAGTGCCA CCTGGTGGTC CCGTTAAGAT GGTCATCAAG 
AATGGAGTCC TAATGCCCAA GCAGAAACAG CGACGCTATC GCACCGAGCG TCCCTTTGCC 
TGTGAACACT GCTCGGCGAG ATTTACGCTG CGTTCGAACA TGGAGCGCCA TGTAAAGCAG 
CAGCATCCGC AGTTCTATGC ACAACGGCAG AGGAGTGGCC ACCATGTGAT GCGGGGCAGG 
GGTGCCTCCA ATGTGGCCGC TGCTGCCGCC GCTGCTGCCG CTGCCGCAGC TGCAACAGTC 
ATGGCTGGCG GACCAGGATC CTCCGGATTC GGCTCCAATC ACCATCATGG CCATGGCCAT 
GGTTCCCATG GTTCCCATGG TCATGCTCCC ATTTCGGAGC AGGTCAAGTG CGCCATTCTG 
GCGCAGCAAT TGAAGGCTCA CAAGAACACC GATCTGTTGC AGCAGGCTTT GGCCCACGGC 
TCCAGCAGTG TGGCCGGCAA TCCTCTCCTG CACTTTGGCT ACCCACTGAC CAACCCCAGT 
CCCATGCACA ATGGTAGCAG CCAGGGCAAT GGCCAGGCCA CGGCCATGGA TGACGATGAG 
CCCAAGTTGA TTATTGACGA GGATGAAAAC GAGCATGATC ATGAGGTGGA AGCAGAAGAC 
GTGGATGACT TCGAAGAGGA TGAAGACGAG GAGGAGATGG ATGAGCCGGA AGATGAGCCA 
GAATTGATTC TAGATGAGCA GCCGGCCGAA AAGGAAGCCG AAGAGGAGCA GGAGCTGCCC 
AAGCCGCTAG AGCAATTGGG TACCAAAGAA GCGGCACAAA AAATGGCCGA AACCATTTTG 
GAGCAGGCCA TCAAGGCGGG CAAGCCATTG AGTCCACCAC CCACCAAGGA GAATGCCTCG 
CCCGCCAATC CAACTGTGGC CACAACAATG CAAGAGCCAG CCATTACGGC GCCCAGTACT 
AATCCCAGCA GTTTGAAAAC GATGATCGCT CAAGCCGAAT ATGTGGGAAA ATCCCTCAAA 
GAAGTGGCCA GTTCACCGTT CAAGGATGAG TCCCAGGACC TGGTTCCAGT GGCCAAGCTG 
GTGGACAATG CCACCAGCCA GAACATGGGC TTCAACAGCT ATTTCCGGCC CAGCGATGTG 
GCCAATCACA TGGAGCAGAG CGACGAGGAG GGCCTCGTGG CCTCCGGAAG CGCCAGTGAG 
AGCAACAACA GCGGTACCGA GGACGTCACC AGCAGCAGTT CCAGCAGTGA GCCCAAGAAG 
AAGTCCGCCT ACAGCCTGGC GCCCAATCGT GTCTCCTGTC CGTATTGCCA GCGCATGTTC 
CCATGGAGCA GCTCGCTGCG CAGGCACATC CTCACCCACA CTGGCCAGAA GCCCTTCAAG 
TGCTCCCACT GCCCGCTGTT GTTCACCACC AAGAGCAACT GCGATCGCCA TCTCTTGCGC 
AAGCACGGCA ATGTGGAGTC GGCCATGTCC GTGTATGTGC CCACTGAGGA TGTGAGTGAA 
CCGATACCAG TGCCCAAGTC CGTCGAGGAG ATCGAGCTGG AGGAGCAGCG ACGGCGGCAG 
GAGGCAGAGC GCGAGAAGGA GCTGGAGCTG GAGCGCGAGC GGGAGTTGGA GCGAGAGCGG 
GAGCTGGAAA GGGAGCGCCA GCTGGAGAAG GAGAAGGAAC GCGAGCGCCA GCAGCTCATC 
CAAAAGCTGG CGGCTCAAAT GAATGCCGCT GCTACTGCTG CTGCCGTCGT TGCTGCAGCC 
TCAGCGGTAA ATGGTGGCGC ATCTGGTGGA CCCCATGGAC CCATTGCAGA TGCCTTGGCT 
GGCGGAGATC TGCCCTACAA GTGCCACCTG TGCGAGGGTT CCTTTGCCGA GCGTCTGCAG 
TGCCTGGAGC ATATCAAGCA GGCCCATGCC CACGAGTATG CCCTGCTCCT GGCCAAGGGA 
GCCATTGAAA CTGAGTCGCT GGAAGCCAAT CCTCACCAGC AGCCGTCGCA GCAGGCCGTG 
CATTCCGATG ACGAGGCTCC GAATGGCGGC GGTAACCGGG GCAAGTATCC CGACTACAGC 
AACCGCAAGG TTATCTGTGC CTTCTGCTTG CGTCGCTTTT GGTCAACGGA GGATCTGCGT 
CGCCACATGC GCACCCATTC GGGCGAGCGG CCATTCCAGT GTGATATCTG CCTGAGGAAG 
TTCACACTGA AGCACAGCAT GCTGCGGCAC ATGAAGAAGC ACAGTGGTAG GGCCCACAAT 
GGCGATACAC CGGGATCCGA TTGCTCCGAC GATGAGCAGG TGAGCAGCCC GCCCAGCACA 
CCACATCCCA CCCAGCCGAC CAGCGCCAAC AACAACAATA GCTGTCACAA TAACAATAAT 
AATGCCAACA ACAATAACAA CAATAACAAC AACAACAACA ACAACAACAG CAGCTCCAAG 
CTGGGACTAA AGCTCCACGA CCTGCTGGAC AAGGCGAGCG AATGGCGGGC CAGTCGCCTG 
GGTGAGCACA AGGAGAACAT GGGCGAGGCC ACGCCCTCGG GAGCAACGGT CGCTGGCTCG 
GATCTGATTG GCAATCTATT GGGCATCAGT GATCAGGGCA TTCTCAACAA GCTGCTCTCC 
TCCGCCGACG AGGCGGCCAA ACTTCTGGGT GTGGACAACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476674.3
CDS	525..6209
Translation	MLAAQQQHNNSTVESEMERQRRDSTTSESSLEHLDLGRTPKKLGGNSGSTQTTSTPHELATVTSSRKRKIRHLQLNHHQQQQHHQQSDLLSDEDVAEAEAEEDEDEEDGDQVAALGSRNLGRHKQRRSGGATTQASIVMDYSSGDASSLRKKFRLNRSAASLSESGFVDASSTTGHSGYLGNSSSATNTTATSGIGASAVAPSPVGGAAINAASSSSGSSSGGSGGSSPQGQCLSSGESGIGAGDEHMKYLCPICEVVSATPHEFTNHIRCHNYANGDTENFTCRICSKVLSSASSLDRHVLVHTGERPFNCRYCHLTFTTNGNMHRHMRTHKQHQVAQSQSQSQQQQSLQQQQQSQQQRRQQQQHQPSQQQQNPAQQQLMGNTLSARAESYESDASCSTDVSSGHSHSRSSSSLNNNNNNSHKANNNLKDLEELEVSTEDQDTENKQRRLKTTINNNIIESEQQEDMDDEEADDADVAMLTSTPDVATLLAGASASGAASRSPTPSPSASPALLLSCPACGASDFETLPALCVHLDAMHSDIPAKCRDCEVIFATHRQLQSHCCRLPNALAGGLPPLLGASSSPLHNEEPEDEEHGDDEDLEQKERLASQSEDFFHQLYLKHKTANGCGAISHPPSPIKHEPADTKDLADIQSILNMTSSSSSFLRNFEQSVNTPNSSQYSLDGRDQEEEAQDAFTSEFRRMKLRGEFPCKLCTAVFPNLRALKGHNRVHLGAVGPAGPFRCNMCPYAVCDKAALVRHMRTHNGDRPYECAVCNYAFTTKANCERHLRNRHGKTSREEVKRAIVYHPAEDAGCEDSKSRLGEDLADTSFRSISPTPPPPPVNESKSQLKHMLLGENHLAPVNQQPPLKIQVKSLDQLVDKKPSAPAPQQQQQQQQQEKSGSALDFSMDVLDLSKKPTGGASLTPAVTRTPTPAAVAPVTPGGVGTPDLAAAIEQQQLLLAQQQLFGAGGEYMQQLFRSLMFQSQTSGFPFFPFMAPPPPQANPEKPPMVSPPNRINPMPVGVGVGVPVPPGGPVKMVIKNGVLMPKQKQRRYRTERPFACEHCSARFTLRSNMERHVKQQHPQFYAQRQRSGHHVMRGRGASNVAAAAAAAAAAAAATVMAGGPGSSGFGSNHHHGHGHGSHGSHGHAPISEQVKCAILAQQLKAHKNTDLLQQALAHGSSSVAGNPLLHFGYPLTNPSPMHNGSSQGNGQATAMDDDEPKLIIDEDENEHDHEVEAEDVDDFEEDEDEEEMDEPEDEPELILDEQPAEKEAEEEQELPKPLEQLGTKEAAQKMAETILEQAIKAGKPLSPPPTKENASPANPTVATTMQEPAITAPSTNPSSLKTMIAQAEYVGKSLKEVASSPFKDESQDLVPVAKLVDNATSQNMGFNSYFRPSDVANHMEQSDEEGLVASGSASESNNSGTEDVTSSSSSSEPKKKSAYSLAPNRVSCPYCQRMFPWSSSLRRHILTHTGQKPFKCSHCPLLFTTKSNCDRHLLRKHGNVESAMSVYVPTEDVSEPIPVPKSVEEIELEEQRRRQEAEREKELELERERELERERELERERQLEKEKERERQQLIQKLAAQMNAAATAAAVVAAASAVNGGASGGPHGPIADALAGGDLPYKCHLCEGSFAERLQCLEHIKQAHAHEYALLLAKGAIETESLEANPHQQPSQQAVHSDDEAPNGGGNRGKYPDYSNRKVICAFCLRRFWSTEDLRRHMRTHSGERPFQCDICLRKFTLKHSMLRHMKKHSGRAHNGDTPGSDCSDDEQVSSPPSTPHPTQPTSANNNNSCHNNNNNANNNNNNNNNNNNNNSSSKLGLKLHDLLDKASEWRASRLGEHKENMGEATPSGATVAGSDLIGNLLGISDQGILNKLLSSADEAAKLLGVDNK

Target ORF information:

RefSeq Version	NM_057326.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebbled (peb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057326.5

ORF Insert Sequence:

ATGCTCGCCG CACAGCAACA GCACAACAAC AGCACAGTGG AATCGGAAAT GGAGCGCCAG 
CGACGGGACT CCACCACATC GGAGTCCTCG CTGGAGCATC TGGATCTGGG ACGTACGCCC 
AAAAAACTGG GCGGTAACAG TGGCTCTACA CAGACCACAT CCACGCCACA TGAGCTGGCT 
ACGGTGACAT CTTCGCGCAA GCGCAAAATT CGCCATTTGC AATTGAATCA CCATCAGCAG 
CAGCAGCATC ACCAGCAGTC GGATCTGCTG AGTGATGAAG ACGTGGCGGA GGCGGAGGCG 
GAGGAGGATG AGGACGAGGA GGATGGCGAC CAGGTGGCCG CCTTGGGCTC AAGGAATCTG 
GGACGGCACA AGCAGCGTCG CAGTGGTGGC GCCACCACAC AGGCATCCAT TGTGATGGAC 
TACAGCAGTG GCGATGCCAG TTCGCTGCGC AAGAAATTTC GTCTGAATCG CTCGGCGGCC 
AGTCTCTCGG AATCGGGATT CGTGGACGCC AGCAGCACCA CTGGCCACAG TGGCTATCTG 
GGTAACAGTA GCAGCGCTAC GAACACTACT GCGACCAGTG GTATTGGTGC TAGCGCAGTG 
GCTCCATCTC CAGTCGGCGG AGCAGCGATC AACGCAGCAT CCAGCAGCAG CGGCAGCAGC 
AGTGGCGGAT CCGGTGGCTC CTCGCCCCAG GGACAGTGCC TCTCCAGCGG TGAATCAGGC 
ATCGGAGCCG GCGACGAGCA TATGAAATAC CTTTGCCCAA TCTGTGAGGT GGTCTCAGCC 
ACGCCGCACG AGTTCACCAA CCACATCCGG TGCCACAATT ACGCCAATGG CGATACGGAG 
AACTTCACCT GCCGCATTTG CTCCAAGGTG CTATCCTCGG CTTCATCCCT GGACAGACAC 
GTGCTGGTTC ACACGGGCGA GCGTCCCTTT AACTGCCGCT ACTGCCACCT GACGTTCACC 
ACCAATGGCA ACATGCACCG TCACATGCGC ACCCACAAAC AGCATCAAGT TGCGCAGTCG 
CAGTCGCAGT CGCAGCAGCA GCAGTCGCTG CAGCAGCAGC AACAGTCGCA GCAGCAGCGC 
CGTCAGCAGC AGCAACATCA GCCGTCGCAG CAGCAGCAGA ATCCCGCCCA GCAGCAATTG 
ATGGGCAACA CGTTATCCGC CCGAGCGGAG AGCTACGAAA GCGATGCCAG CTGCTCCACG 
GACGTGTCCA GTGGACATAG TCACAGTCGC AGCAGCAGTT CCCTTAACAA CAACAACAAC 
AACTCGCACA AGGCTAACAA CAATCTAAAG GACTTGGAGG AGCTGGAGGT TTCGACTGAG 
GATCAGGATA CGGAGAACAA GCAGCGTCGA CTGAAGACCA CCATCAACAA CAACATCATC 
GAGAGTGAGC AGCAGGAGGA TATGGATGAT GAGGAGGCCG ACGATGCGGA TGTGGCCATG 
CTGACCAGCA CTCCCGATGT CGCCACTCTT TTGGCCGGAG CCAGTGCATC TGGCGCCGCC 
TCGCGTTCCC CTACCCCATC GCCATCCGCC TCACCTGCCT TGCTCCTAAG TTGTCCCGCC 
TGCGGAGCCT CCGATTTCGA GACCCTGCCC GCTCTCTGCG TCCATTTGGA TGCAATGCAC 
TCAGACATAC CAGCCAAGTG TCGGGACTGC GAGGTGATCT TTGCCACCCA TCGTCAGTTG 
CAGTCGCACT GTTGTCGCTT ACCCAACGCT CTGGCCGGTG GCTTACCGCC GCTTCTCGGA 
GCCAGCAGTT CGCCATTGCA CAACGAGGAG CCGGAGGATG AGGAGCACGG TGACGATGAG 
GATCTCGAGC AGAAGGAGAG GTTAGCGAGT CAAAGCGAGG ACTTTTTCCA TCAGTTGTAT 
CTGAAGCACA AGACAGCAAA TGGATGCGGA GCCATCTCGC ATCCGCCATC GCCCATCAAA 
CATGAGCCGG CGGACACCAA GGATCTGGCC GATATCCAGA GCATCCTGAA CATGACCAGC 
TCCAGTTCCA GTTTTCTGCG CAACTTTGAG CAGTCCGTGA ACACGCCCAA TTCGAGTCAG 
TACAGCCTGG ACGGTCGGGA TCAGGAGGAG GAGGCACAGG ACGCGTTCAC CTCGGAGTTC 
CGTCGCATGA AGCTGCGCGG CGAGTTTCCC TGCAAGCTGT GCACGGCCGT TTTCCCGAAC 
CTTCGTGCCC TCAAAGGTCA CAATCGGGTA CATTTGGGTG CAGTGGGTCC AGCCGGACCC 
TTTCGCTGCA ACATGTGTCC CTACGCCGTT TGCGACAAGG CCGCCCTGGT GCGTCACATG 
CGTACCCACA ACGGAGATCG TCCGTACGAG TGCGCCGTGT GCAACTATGC CTTCACCACC 
AAGGCGAACT GTGAACGTCA TCTGAGGAAT CGCCATGGGA AGACCAGTCG CGAGGAGGTG 
AAGCGAGCCA TTGTCTACCA TCCGGCGGAG GATGCTGGCT GTGAGGACAG CAAATCGCGT 
CTGGGTGAAG ATCTGGCTGA TACGAGTTTC CGTTCGATCA GTCCCACGCC ACCGCCTCCT 
CCGGTAAATG AGAGCAAATC CCAGCTGAAG CACATGCTTC TCGGCGAGAA TCACCTGGCT 
CCTGTTAACC AACAGCCGCC GCTGAAGATC CAGGTGAAGA GCCTCGACCA ACTAGTGGAC 
AAGAAGCCAT CGGCACCAGC TCCCCAGCAG CAACAGCAGC AGCAGCAGCA GGAGAAGAGC 
GGATCTGCCC TAGACTTCAG CATGGATGTT TTGGATCTCA GCAAGAAGCC AACTGGCGGA 
GCATCGCTGA CACCGGCGGT CACTCGCACA CCGACGCCCG CTGCAGTTGC TCCAGTGACG 
CCTGGCGGCG TTGGAACTCC TGACCTGGCC GCCGCCATTG AGCAGCAACA GCTGCTACTG 
GCCCAGCAGC AACTCTTTGG TGCTGGCGGC GAGTATATGC AGCAGCTATT CCGCAGTCTC 
ATGTTCCAGT CCCAGACGTC GGGATTCCCG TTCTTTCCCT TTATGGCACC ACCACCGCCA 
CAAGCAAATC CGGAGAAGCC ACCGATGGTG AGTCCACCAA ACCGTATCAA TCCCATGCCC 
GTGGGCGTTG GAGTTGGCGT GCCAGTGCCA CCTGGTGGTC CCGTTAAGAT GGTCATCAAG 
AATGGAGTCC TAATGCCCAA GCAGAAACAG CGACGCTATC GCACCGAGCG TCCCTTTGCC 
TGTGAACACT GCTCGGCGAG ATTTACGCTG CGTTCGAACA TGGAGCGCCA TGTAAAGCAG 
CAGCATCCGC AGTTCTATGC ACAACGGCAG AGGAGTGGCC ACCATGTGAT GCGGGGCAGG 
GGTGCCTCCA ATGTGGCCGC TGCTGCCGCC GCTGCTGCCG CTGCCGCAGC TGCAACAGTC 
ATGGCTGGCG GACCAGGATC CTCCGGATTC GGCTCCAATC ACCATCATGG CCATGGCCAT 
GGTTCCCATG GTTCCCATGG TCATGCTCCC ATTTCGGAGC AGGTCAAGTG CGCCATTCTG 
GCGCAGCAAT TGAAGGCTCA CAAGAACACC GATCTGTTGC AGCAGGCTTT GGCCCACGGC 
TCCAGCAGTG TGGCCGGCAA TCCTCTCCTG CACTTTGGCT ACCCACTGAC CAACCCCAGT 
CCCATGCACA ATGGTAGCAG CCAGGGCAAT GGCCAGGCCA CGGCCATGGA TGACGATGAG 
CCCAAGTTGA TTATTGACGA GGATGAAAAC GAGCATGATC ATGAGGTGGA AGCAGAAGAC 
GTGGATGACT TCGAAGAGGA TGAAGACGAG GAGGAGATGG ATGAGCCGGA AGATGAGCCA 
GAATTGATTC TAGATGAGCA GCCGGCCGAA AAGGAAGCCG AAGAGGAGCA GGAGCTGCCC 
AAGCCGCTAG AGCAATTGGG TACCAAAGAA GCGGCACAAA AAATGGCCGA AACCATTTTG 
GAGCAGGCCA TCAAGGCGGG CAAGCCATTG AGTCCACCAC CCACCAAGGA GAATGCCTCG 
CCCGCCAATC CAACTGTGGC CACAACAATG CAAGAGCCAG CCATTACGGC GCCCAGTACT 
AATCCCAGCA GTTTGAAAAC GATGATCGCT CAAGCCGAAT ATGTGGGAAA ATCCCTCAAA 
GAAGTGGCCA GTTCACCGTT CAAGGATGAG TCCCAGGACC TGGTTCCAGT GGCCAAGCTG 
GTGGACAATG CCACCAGCCA GAACATGGGC TTCAACAGCT ATTTCCGGCC CAGCGATGTG 
GCCAATCACA TGGAGCAGAG CGACGAGGAG GGCCTCGTGG CCTCCGGAAG CGCCAGTGAG 
AGCAACAACA GCGGTACCGA GGACGTCACC AGCAGCAGTT CCAGCAGTGA GCCCAAGAAG 
AAGTCCGCCT ACAGCCTGGC GCCCAATCGT GTCTCCTGTC CGTATTGCCA GCGCATGTTC 
CCATGGAGCA GCTCGCTGCG CAGGCACATC CTCACCCACA CTGGCCAGAA GCCCTTCAAG 
TGCTCCCACT GCCCGCTGTT GTTCACCACC AAGAGCAACT GCGATCGCCA TCTCTTGCGC 
AAGCACGGCA ATGTGGAGTC GGCCATGTCC GTGTATGTGC CCACTGAGGA TGTGAGTGAA 
CCGATACCAG TGCCCAAGTC CGTCGAGGAG ATCGAGCTGG AGGAGCAGCG ACGGCGGCAG 
GAGGCAGAGC GCGAGAAGGA GCTGGAGCTG GAGCGCGAGC GGGAGTTGGA GCGAGAGCGG 
GAGCTGGAAA GGGAGCGCCA GCTGGAGAAG GAGAAGGAAC GCGAGCGCCA GCAGCTCATC 
CAAAAGCTGG CGGCTCAAAT GAATGCCGCT GCTACTGCTG CTGCCGTCGT TGCTGCAGCC 
TCAGCGGTAA ATGGTGGCGC ATCTGGTGGA CCCCATGGAC CCATTGCAGA TGCCTTGGCT 
GGCGGAGATC TGCCCTACAA GTGCCACCTG TGCGAGGGTT CCTTTGCCGA GCGTCTGCAG 
TGCCTGGAGC ATATCAAGCA GGCCCATGCC CACGAGTATG CCCTGCTCCT GGCCAAGGGA 
GCCATTGAAA CTGAGTCGCT GGAAGCCAAT CCTCACCAGC AGCCGTCGCA GCAGGCCGTG 
CATTCCGATG ACGAGGCTCC GAATGGCGGC GGTAACCGGG GCAAGTATCC CGACTACAGC 
AACCGCAAGG TTATCTGTGC CTTCTGCTTG CGTCGCTTTT GGTCAACGGA GGATCTGCGT 
CGCCACATGC GCACCCATTC GGGCGAGCGG CCATTCCAGT GTGATATCTG CCTGAGGAAG 
TTCACACTGA AGCACAGCAT GCTGCGGCAC ATGAAGAAGC ACAGTGGTAG GGCCCACAAT 
GGCGATACAC CGGGATCCGA TTGCTCCGAC GATGAGCAGG TGAGCAGCCC GCCCAGCACA 
CCACATCCCA CCCAGCCGAC CAGCGCCAAC AACAACAATA GCTGTCACAA TAACAATAAT 
AATGCCAACA ACAATAACAA CAATAACAAC AACAACAACA ACAACAACAG CAGCTCCAAG 
CTGGGACTAA AGCTCCACGA CCTGCTGGAC AAGGCGAGCG AATGGCGGGC CAGTCGCCTG 
GGTGAGCACA AGGAGAACAT GGGCGAGGCC ACGCCCTCGG GAGCAACGGT CGCTGGCTCG 
GATCTGATTG GCAATCTATT GGGCATCAGT GATCAGGGCA TTCTCAACAA GCTGCTCTCC 
TCCGCCGACG AGGCGGCCAA ACTTCTGGGT GTGGACAACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

peb ( NM_001014722.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014722.1 Drosophila melanogaster pebbled (peb), transcript variant B, mRNA.

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CloneID	OFa00787
Clone ID Related Accession (Same CDS sequence)	NM_057326.5 , NM_001014722.2
Accession Version	NM_001014722.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pebbled, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001014722.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCGCCG CACAGCAACA GCACAACAAC AGCACAGTGG AATCGGAAAT GGAGCGCCAG 
CGACGGGACT CCACCACATC GGAGTCCTCG CTGGAGCATC TGGATCTGGG ACGTACGCCC 
AAAAAACTGG GCGGTAACAG TGGCTCTACA CAGACCACAT CCACGCCACA TGAGCTGGCT 
ACGGTGACAT CTTCGCGCAA GCGCAAAATT CGCCATTTGC AATTGAATCA CCATCAGCAG 
CAGCAGCATC ACCAGCAGTC GGATCTGCTG AGTGATGAAG ACGTGGCGGA GGCGGAGGCG 
GAGGAGGATG AGGACGAGGA GGATGGCGAC CAGGTGGCCG CCTTGGGCTC AAGGAATCTG 
GGACGGCACA AGCAGCGTCG CAGTGGTGGC GCCACCACAC AGGCATCCAT TGTGATGGAC 
TACAGCAGTG GCGATGCCAG TTCGCTGCGC AAGAAATTTC GTCTGAATCG CTCGGCGGCC 
AGTCTCTCGG AATCGGGATT CGTGGACGCC AGCAGCACCA CTGGCCACAG TGGCTATCTG 
GGTAACAGTA GCAGCGCTAC GAACACTACT GCGACCAGTG GTATTGGTGC TAGCGCAGTG 
GCTCCATCTC CAGTCGGCGG AGCAGCGATC AACGCAGCAT CCAGCAGCAG CGGCAGCAGC 
AGTGGCGGAT CCGGTGGCTC CTCGCCCCAG GGACAGTGCC TCTCCAGCGG TGAATCAGGC 
ATCGGAGCCG GCGACGAGCA TATGAAATAC CTTTGCCCAA TCTGTGAGGT GGTCTCAGCC 
ACGCCGCACG AGTTCACCAA CCACATCCGG TGCCACAATT ACGCCAATGG CGATACGGAG 
AACTTCACCT GCCGCATTTG CTCCAAGGTG CTATCCTCGG CTTCATCCCT GGACAGACAC 
GTGCTGGTTC ACACGGGCGA GCGTCCCTTT AACTGCCGCT ACTGCCACCT GACGTTCACC 
ACCAATGGCA ACATGCACCG TCACATGCGC ACCCACAAAC AGCATCAAGT TGCGCAGTCG 
CAGTCGCAGT CGCAGCAGCA GCAGTCGCTG CAGCAGCAGC AACAGTCGCA GCAGCAGCGC 
CGTCAGCAGC AGCAACATCA GCCGTCGCAG CAGCAGCAGA ATCCCGCCCA GCAGCAATTG 
ATGGGCAACA CGTTATCCGC CCGAGCGGAG AGCTACGAAA GCGATGCCAG CTGCTCCACG 
GACGTGTCCA GTGGACATAG TCACAGTCGC AGCAGCAGTT CCCTTAACAA CAACAACAAC 
AACTCGCACA AGGCTAACAA CAATCTAAAG GACTTGGAGG AGCTGGAGGT TTCGACTGAG 
GATCAGGATA CGGAGAACAA GCAGCGTCGA CTGAAGACCA CCATCAACAA CAACATCATC 
GAGAGTGAGC AGCAGGAGGA TATGGATGAT GAGGAGGCCG ACGATGCGGA TGTGGCCATG 
CTGACCAGCA CTCCCGATGT CGCCACTCTT TTGGCCGGAG CCAGTGCATC TGGCGCCGCC 
TCGCGTTCCC CTACCCCATC GCCATCCGCC TCACCTGCCT TGCTCCTAAG TTGTCCCGCC 
TGCGGAGCCT CCGATTTCGA GACCCTGCCC GCTCTCTGCG TCCATTTGGA TGCAATGCAC 
TCAGACATAC CAGCCAAGTG TCGGGACTGC GAGGTGATCT TTGCCACCCA TCGTCAGTTG 
CAGTCGCACT GTTGTCGCTT ACCCAACGCT CTGGCCGGTG GCTTACCGCC GCTTCTCGGA 
GCCAGCAGTT CGCCATTGCA CAACGAGGAG CCGGAGGATG AGGAGCACGG TGACGATGAG 
GATCTCGAGC AGAAGGAGAG GTTAGCGAGT CAAAGCGAGG ACTTTTTCCA TCAGTTGTAT 
CTGAAGCACA AGACAGCAAA TGGATGCGGA GCCATCTCGC ATCCGCCATC GCCCATCAAA 
CATGAGCCGG CGGACACCAA GGATCTGGCC GATATCCAGA GCATCCTGAA CATGACCAGC 
TCCAGTTCCA GTTTTCTGCG CAACTTTGAG CAGTCCGTGA ACACGCCCAA TTCGAGTCAG 
TACAGCCTGG ACGGTCGGGA TCAGGAGGAG GAGGCACAGG ACGCGTTCAC CTCGGAGTTC 
CGTCGCATGA AGCTGCGCGG CGAGTTTCCC TGCAAGCTGT GCACGGCCGT TTTCCCGAAC 
CTTCGTGCCC TCAAAGGTCA CAATCGGGTA CATTTGGGTG CAGTGGGTCC AGCCGGACCC 
TTTCGCTGCA ACATGTGTCC CTACGCCGTT TGCGACAAGG CCGCCCTGGT GCGTCACATG 
CGTACCCACA ACGGAGATCG TCCGTACGAG TGCGCCGTGT GCAACTATGC CTTCACCACC 
AAGGCGAACT GTGAACGTCA TCTGAGGAAT CGCCATGGGA AGACCAGTCG CGAGGAGGTG 
AAGCGAGCCA TTGTCTACCA TCCGGCGGAG GATGCTGGCT GTGAGGACAG CAAATCGCGT 
CTGGGTGAAG ATCTGGCTGA TACGAGTTTC CGTTCGATCA GTCCCACGCC ACCGCCTCCT 
CCGGTAAATG AGAGCAAATC CCAGCTGAAG CACATGCTTC TCGGCGAGAA TCACCTGGCT 
CCTGTTAACC AACAGCCGCC GCTGAAGATC CAGGTGAAGA GCCTCGACCA ACTAGTGGAC 
AAGAAGCCAT CGGCACCAGC TCCCCAGCAG CAACAGCAGC AGCAGCAGCA GGAGAAGAGC 
GGATCTGCCC TAGACTTCAG CATGGATGTT TTGGATCTCA GCAAGAAGCC AACTGGCGGA 
GCATCGCTGA CACCGGCGGT CACTCGCACA CCGACGCCCG CTGCAGTTGC TCCAGTGACG 
CCTGGCGGCG TTGGAACTCC TGACCTGGCC GCCGCCATTG AGCAGCAACA GCTGCTACTG 
GCCCAGCAGC AACTCTTTGG TGCTGGCGGC GAGTATATGC AGCAGCTATT CCGCAGTCTC 
ATGTTCCAGT CCCAGACGTC GGGATTCCCG TTCTTTCCCT TTATGGCACC ACCACCGCCA 
CAAGCAAATC CGGAGAAGCC ACCGATGGTG AGTCCACCAA ACCGTATCAA TCCCATGCCC 
GTGGGCGTTG GAGTTGGCGT GCCAGTGCCA CCTGGTGGTC CCGTTAAGAT GGTCATCAAG 
AATGGAGTCC TAATGCCCAA GCAGAAACAG CGACGCTATC GCACCGAGCG TCCCTTTGCC 
TGTGAACACT GCTCGGCGAG ATTTACGCTG CGTTCGAACA TGGAGCGCCA TGTAAAGCAG 
CAGCATCCGC AGTTCTATGC ACAACGGCAG AGGAGTGGCC ACCATGTGAT GCGGGGCAGG 
GGTGCCTCCA ATGTGGCCGC TGCTGCCGCC GCTGCTGCCG CTGCCGCAGC TGCAACAGTC 
ATGGCTGGCG GACCAGGATC CTCCGGATTC GGCTCCAATC ACCATCATGG CCATGGCCAT 
GGTTCCCATG GTTCCCATGG TCATGCTCCC ATTTCGGAGC AGGTCAAGTG CGCCATTCTG 
GCGCAGCAAT TGAAGGCTCA CAAGAACACC GATCTGTTGC AGCAGGCTTT GGCCCACGGC 
TCCAGCAGTG TGGCCGGCAA TCCTCTCCTG CACTTTGGCT ACCCACTGAC CAACCCCAGT 
CCCATGCACA ATGGTAGCAG CCAGGGCAAT GGCCAGGCCA CGGCCATGGA TGACGATGAG 
CCCAAGTTGA TTATTGACGA GGATGAAAAC GAGCATGATC ATGAGGTGGA AGCAGAAGAC 
GTGGATGACT TCGAAGAGGA TGAAGACGAG GAGGAGATGG ATGAGCCGGA AGATGAGCCA 
GAATTGATTC TAGATGAGCA GCCGGCCGAA AAGGAAGCCG AAGAGGAGCA GGAGCTGCCC 
AAGCCGCTAG AGCAATTGGG TACCAAAGAA GCGGCACAAA AAATGGCCGA AACCATTTTG 
GAGCAGGCCA TCAAGGCGGG CAAGCCATTG AGTCCACCAC CCACCAAGGA GAATGCCTCG 
CCCGCCAATC CAACTGTGGC CACAACAATG CAAGAGCCAG CCATTACGGC GCCCAGTACT 
AATCCCAGCA GTTTGAAAAC GATGATCGCT CAAGCCGAAT ATGTGGGAAA ATCCCTCAAA 
GAAGTGGCCA GTTCACCGTT CAAGGATGAG TCCCAGGACC TGGTTCCAGT GGCCAAGCTG 
GTGGACAATG CCACCAGCCA GAACATGGGC TTCAACAGCT ATTTCCGGCC CAGCGATGTG 
GCCAATCACA TGGAGCAGAG CGACGAGGAG GGCCTCGTGG CCTCCGGAAG CGCCAGTGAG 
AGCAACAACA GCGGTACCGA GGACGTCACC AGCAGCAGTT CCAGCAGTGA GCCCAAGAAG 
AAGTCCGCCT ACAGCCTGGC GCCCAATCGT GTCTCCTGTC CGTATTGCCA GCGCATGTTC 
CCATGGAGCA GCTCGCTGCG CAGGCACATC CTCACCCACA CTGGCCAGAA GCCCTTCAAG 
TGCTCCCACT GCCCGCTGTT GTTCACCACC AAGAGCAACT GCGATCGCCA TCTCTTGCGC 
AAGCACGGCA ATGTGGAGTC GGCCATGTCC GTGTATGTGC CCACTGAGGA TGTGAGTGAA 
CCGATACCAG TGCCCAAGTC CGTCGAGGAG ATCGAGCTGG AGGAGCAGCG ACGGCGGCAG 
GAGGCAGAGC GCGAGAAGGA GCTGGAGCTG GAGCGCGAGC GGGAGTTGGA GCGAGAGCGG 
GAGCTGGAAA GGGAGCGCCA GCTGGAGAAG GAGAAGGAAC GCGAGCGCCA GCAGCTCATC 
CAAAAGCTGG CGGCTCAAAT GAATGCCGCT GCTACTGCTG CTGCCGTCGT TGCTGCAGCC 
TCAGCGGTAA ATGGTGGCGC ATCTGGTGGA CCCCATGGAC CCATTGCAGA TGCCTTGGCT 
GGCGGAGATC TGCCCTACAA GTGCCACCTG TGCGAGGGTT CCTTTGCCGA GCGTCTGCAG 
TGCCTGGAGC ATATCAAGCA GGCCCATGCC CACGAGTATG CCCTGCTCCT GGCCAAGGGA 
GCCATTGAAA CTGAGTCGCT GGAAGCCAAT CCTCACCAGC AGCCGTCGCA GCAGGCCGTG 
CATTCCGATG ACGAGGCTCC GAATGGCGGC GGTAACCGGG GCAAGTATCC CGACTACAGC 
AACCGCAAGG TTATCTGTGC CTTCTGCTTG CGTCGCTTTT GGTCAACGGA GGATCTGCGT 
CGCCACATGC GCACCCATTC GGGCGAGCGG CCATTCCAGT GTGATATCTG CCTGAGGAAG 
TTCACACTGA AGCACAGCAT GCTGCGGCAC ATGAAGAAGC ACAGTGGTAG GGCCCACAAT 
GGCGATACAC CGGGATCCGA TTGCTCCGAC GATGAGCAGG TGAGCAGCCC GCCCAGCACA 
CCACATCCCA CCCAGCCGAC CAGCGCCAAC AACAACAATA GCTGTCACAA TAACAATAAT 
AATGCCAACA ACAATAACAA CAATAACAAC AACAACAACA ACAACAACAG CAGCTCCAAG 
CTGGGACTAA AGCTCCACGA CCTGCTGGAC AAGGCGAGCG AATGGCGGGC CAGTCGCCTG 
GGTGAGCACA AGGAGAACAT GGGCGAGGCC ACGCCCTCGG GAGCAACGGT CGCTGGCTCG 
GATCTGATTG GCAATCTATT GGGCATCAGT GATCAGGGCA TTCTCAACAA GCTGCTCTCC 
TCCGCCGACG AGGCGGCCAA ACTTCTGGGT GTGGACAACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014722.1
CDS	520..6204
Translation	MLAAQQQHNNSTVESEMERQRRDSTTSESSLEHLDLGRTPKKLGGNSGSTQTTSTPHELATVTSSRKRKIRHLQLNHHQQQQHHQQSDLLSDEDVAEAEAEEDEDEEDGDQVAALGSRNLGRHKQRRSGGATTQASIVMDYSSGDASSLRKKFRLNRSAASLSESGFVDASSTTGHSGYLGNSSSATNTTATSGIGASAVAPSPVGGAAINAASSSSGSSSGGSGGSSPQGQCLSSGESGIGAGDEHMKYLCPICEVVSATPHEFTNHIRCHNYANGDTENFTCRICSKVLSSASSLDRHVLVHTGERPFNCRYCHLTFTTNGNMHRHMRTHKQHQVAQSQSQSQQQQSLQQQQQSQQQRRQQQQHQPSQQQQNPAQQQLMGNTLSARAESYESDASCSTDVSSGHSHSRSSSSLNNNNNNSHKANNNLKDLEELEVSTEDQDTENKQRRLKTTINNNIIESEQQEDMDDEEADDADVAMLTSTPDVATLLAGASASGAASRSPTPSPSASPALLLSCPACGASDFETLPALCVHLDAMHSDIPAKCRDCEVIFATHRQLQSHCCRLPNALAGGLPPLLGASSSPLHNEEPEDEEHGDDEDLEQKERLASQSEDFFHQLYLKHKTANGCGAISHPPSPIKHEPADTKDLADIQSILNMTSSSSSFLRNFEQSVNTPNSSQYSLDGRDQEEEAQDAFTSEFRRMKLRGEFPCKLCTAVFPNLRALKGHNRVHLGAVGPAGPFRCNMCPYAVCDKAALVRHMRTHNGDRPYECAVCNYAFTTKANCERHLRNRHGKTSREEVKRAIVYHPAEDAGCEDSKSRLGEDLADTSFRSISPTPPPPPVNESKSQLKHMLLGENHLAPVNQQPPLKIQVKSLDQLVDKKPSAPAPQQQQQQQQQEKSGSALDFSMDVLDLSKKPTGGASLTPAVTRTPTPAAVAPVTPGGVGTPDLAAAIEQQQLLLAQQQLFGAGGEYMQQLFRSLMFQSQTSGFPFFPFMAPPPPQANPEKPPMVSPPNRINPMPVGVGVGVPVPPGGPVKMVIKNGVLMPKQKQRRYRTERPFACEHCSARFTLRSNMERHVKQQHPQFYAQRQRSGHHVMRGRGASNVAAAAAAAAAAAAATVMAGGPGSSGFGSNHHHGHGHGSHGSHGHAPISEQVKCAILAQQLKAHKNTDLLQQALAHGSSSVAGNPLLHFGYPLTNPSPMHNGSSQGNGQATAMDDDEPKLIIDEDENEHDHEVEAEDVDDFEEDEDEEEMDEPEDEPELILDEQPAEKEAEEEQELPKPLEQLGTKEAAQKMAETILEQAIKAGKPLSPPPTKENASPANPTVATTMQEPAITAPSTNPSSLKTMIAQAEYVGKSLKEVASSPFKDESQDLVPVAKLVDNATSQNMGFNSYFRPSDVANHMEQSDEEGLVASGSASESNNSGTEDVTSSSSSSEPKKKSAYSLAPNRVSCPYCQRMFPWSSSLRRHILTHTGQKPFKCSHCPLLFTTKSNCDRHLLRKHGNVESAMSVYVPTEDVSEPIPVPKSVEEIELEEQRRRQEAEREKELELERERELERERELERERQLEKEKERERQQLIQKLAAQMNAAATAAAVVAAASAVNGGASGGPHGPIADALAGGDLPYKCHLCEGSFAERLQCLEHIKQAHAHEYALLLAKGAIETESLEANPHQQPSQQAVHSDDEAPNGGGNRGKYPDYSNRKVICAFCLRRFWSTEDLRRHMRTHSGERPFQCDICLRKFTLKHSMLRHMKKHSGRAHNGDTPGSDCSDDEQVSSPPSTPHPTQPTSANNNNSCHNNNNNANNNNNNNNNNNNNNSSSKLGLKLHDLLDKASEWRASRLGEHKENMGEATPSGATVAGSDLIGNLLGISDQGILNKLLSSADEAAKLLGVDNK

Target ORF information:

RefSeq Version	NM_001014722.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pebbled (peb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014722.2

ORF Insert Sequence:

ATGCTCGCCG CACAGCAACA GCACAACAAC AGCACAGTGG AATCGGAAAT GGAGCGCCAG 
CGACGGGACT CCACCACATC GGAGTCCTCG CTGGAGCATC TGGATCTGGG ACGTACGCCC 
AAAAAACTGG GCGGTAACAG TGGCTCTACA CAGACCACAT CCACGCCACA TGAGCTGGCT 
ACGGTGACAT CTTCGCGCAA GCGCAAAATT CGCCATTTGC AATTGAATCA CCATCAGCAG 
CAGCAGCATC ACCAGCAGTC GGATCTGCTG AGTGATGAAG ACGTGGCGGA GGCGGAGGCG 
GAGGAGGATG AGGACGAGGA GGATGGCGAC CAGGTGGCCG CCTTGGGCTC AAGGAATCTG 
GGACGGCACA AGCAGCGTCG CAGTGGTGGC GCCACCACAC AGGCATCCAT TGTGATGGAC 
TACAGCAGTG GCGATGCCAG TTCGCTGCGC AAGAAATTTC GTCTGAATCG CTCGGCGGCC 
AGTCTCTCGG AATCGGGATT CGTGGACGCC AGCAGCACCA CTGGCCACAG TGGCTATCTG 
GGTAACAGTA GCAGCGCTAC GAACACTACT GCGACCAGTG GTATTGGTGC TAGCGCAGTG 
GCTCCATCTC CAGTCGGCGG AGCAGCGATC AACGCAGCAT CCAGCAGCAG CGGCAGCAGC 
AGTGGCGGAT CCGGTGGCTC CTCGCCCCAG GGACAGTGCC TCTCCAGCGG TGAATCAGGC 
ATCGGAGCCG GCGACGAGCA TATGAAATAC CTTTGCCCAA TCTGTGAGGT GGTCTCAGCC 
ACGCCGCACG AGTTCACCAA CCACATCCGG TGCCACAATT ACGCCAATGG CGATACGGAG 
AACTTCACCT GCCGCATTTG CTCCAAGGTG CTATCCTCGG CTTCATCCCT GGACAGACAC 
GTGCTGGTTC ACACGGGCGA GCGTCCCTTT AACTGCCGCT ACTGCCACCT GACGTTCACC 
ACCAATGGCA ACATGCACCG TCACATGCGC ACCCACAAAC AGCATCAAGT TGCGCAGTCG 
CAGTCGCAGT CGCAGCAGCA GCAGTCGCTG CAGCAGCAGC AACAGTCGCA GCAGCAGCGC 
CGTCAGCAGC AGCAACATCA GCCGTCGCAG CAGCAGCAGA ATCCCGCCCA GCAGCAATTG 
ATGGGCAACA CGTTATCCGC CCGAGCGGAG AGCTACGAAA GCGATGCCAG CTGCTCCACG 
GACGTGTCCA GTGGACATAG TCACAGTCGC AGCAGCAGTT CCCTTAACAA CAACAACAAC 
AACTCGCACA AGGCTAACAA CAATCTAAAG GACTTGGAGG AGCTGGAGGT TTCGACTGAG 
GATCAGGATA CGGAGAACAA GCAGCGTCGA CTGAAGACCA CCATCAACAA CAACATCATC 
GAGAGTGAGC AGCAGGAGGA TATGGATGAT GAGGAGGCCG ACGATGCGGA TGTGGCCATG 
CTGACCAGCA CTCCCGATGT CGCCACTCTT TTGGCCGGAG CCAGTGCATC TGGCGCCGCC 
TCGCGTTCCC CTACCCCATC GCCATCCGCC TCACCTGCCT TGCTCCTAAG TTGTCCCGCC 
TGCGGAGCCT CCGATTTCGA GACCCTGCCC GCTCTCTGCG TCCATTTGGA TGCAATGCAC 
TCAGACATAC CAGCCAAGTG TCGGGACTGC GAGGTGATCT TTGCCACCCA TCGTCAGTTG 
CAGTCGCACT GTTGTCGCTT ACCCAACGCT CTGGCCGGTG GCTTACCGCC GCTTCTCGGA 
GCCAGCAGTT CGCCATTGCA CAACGAGGAG CCGGAGGATG AGGAGCACGG TGACGATGAG 
GATCTCGAGC AGAAGGAGAG GTTAGCGAGT CAAAGCGAGG ACTTTTTCCA TCAGTTGTAT 
CTGAAGCACA AGACAGCAAA TGGATGCGGA GCCATCTCGC ATCCGCCATC GCCCATCAAA 
CATGAGCCGG CGGACACCAA GGATCTGGCC GATATCCAGA GCATCCTGAA CATGACCAGC 
TCCAGTTCCA GTTTTCTGCG CAACTTTGAG CAGTCCGTGA ACACGCCCAA TTCGAGTCAG 
TACAGCCTGG ACGGTCGGGA TCAGGAGGAG GAGGCACAGG ACGCGTTCAC CTCGGAGTTC 
CGTCGCATGA AGCTGCGCGG CGAGTTTCCC TGCAAGCTGT GCACGGCCGT TTTCCCGAAC 
CTTCGTGCCC TCAAAGGTCA CAATCGGGTA CATTTGGGTG CAGTGGGTCC AGCCGGACCC 
TTTCGCTGCA ACATGTGTCC CTACGCCGTT TGCGACAAGG CCGCCCTGGT GCGTCACATG 
CGTACCCACA ACGGAGATCG TCCGTACGAG TGCGCCGTGT GCAACTATGC CTTCACCACC 
AAGGCGAACT GTGAACGTCA TCTGAGGAAT CGCCATGGGA AGACCAGTCG CGAGGAGGTG 
AAGCGAGCCA TTGTCTACCA TCCGGCGGAG GATGCTGGCT GTGAGGACAG CAAATCGCGT 
CTGGGTGAAG ATCTGGCTGA TACGAGTTTC CGTTCGATCA GTCCCACGCC ACCGCCTCCT 
CCGGTAAATG AGAGCAAATC CCAGCTGAAG CACATGCTTC TCGGCGAGAA TCACCTGGCT 
CCTGTTAACC AACAGCCGCC GCTGAAGATC CAGGTGAAGA GCCTCGACCA ACTAGTGGAC 
AAGAAGCCAT CGGCACCAGC TCCCCAGCAG CAACAGCAGC AGCAGCAGCA GGAGAAGAGC 
GGATCTGCCC TAGACTTCAG CATGGATGTT TTGGATCTCA GCAAGAAGCC AACTGGCGGA 
GCATCGCTGA CACCGGCGGT CACTCGCACA CCGACGCCCG CTGCAGTTGC TCCAGTGACG 
CCTGGCGGCG TTGGAACTCC TGACCTGGCC GCCGCCATTG AGCAGCAACA GCTGCTACTG 
GCCCAGCAGC AACTCTTTGG TGCTGGCGGC GAGTATATGC AGCAGCTATT CCGCAGTCTC 
ATGTTCCAGT CCCAGACGTC GGGATTCCCG TTCTTTCCCT TTATGGCACC ACCACCGCCA 
CAAGCAAATC CGGAGAAGCC ACCGATGGTG AGTCCACCAA ACCGTATCAA TCCCATGCCC 
GTGGGCGTTG GAGTTGGCGT GCCAGTGCCA CCTGGTGGTC CCGTTAAGAT GGTCATCAAG 
AATGGAGTCC TAATGCCCAA GCAGAAACAG CGACGCTATC GCACCGAGCG TCCCTTTGCC 
TGTGAACACT GCTCGGCGAG ATTTACGCTG CGTTCGAACA TGGAGCGCCA TGTAAAGCAG 
CAGCATCCGC AGTTCTATGC ACAACGGCAG AGGAGTGGCC ACCATGTGAT GCGGGGCAGG 
GGTGCCTCCA ATGTGGCCGC TGCTGCCGCC GCTGCTGCCG CTGCCGCAGC TGCAACAGTC 
ATGGCTGGCG GACCAGGATC CTCCGGATTC GGCTCCAATC ACCATCATGG CCATGGCCAT 
GGTTCCCATG GTTCCCATGG TCATGCTCCC ATTTCGGAGC AGGTCAAGTG CGCCATTCTG 
GCGCAGCAAT TGAAGGCTCA CAAGAACACC GATCTGTTGC AGCAGGCTTT GGCCCACGGC 
TCCAGCAGTG TGGCCGGCAA TCCTCTCCTG CACTTTGGCT ACCCACTGAC CAACCCCAGT 
CCCATGCACA ATGGTAGCAG CCAGGGCAAT GGCCAGGCCA CGGCCATGGA TGACGATGAG 
CCCAAGTTGA TTATTGACGA GGATGAAAAC GAGCATGATC ATGAGGTGGA AGCAGAAGAC 
GTGGATGACT TCGAAGAGGA TGAAGACGAG GAGGAGATGG ATGAGCCGGA AGATGAGCCA 
GAATTGATTC TAGATGAGCA GCCGGCCGAA AAGGAAGCCG AAGAGGAGCA GGAGCTGCCC 
AAGCCGCTAG AGCAATTGGG TACCAAAGAA GCGGCACAAA AAATGGCCGA AACCATTTTG 
GAGCAGGCCA TCAAGGCGGG CAAGCCATTG AGTCCACCAC CCACCAAGGA GAATGCCTCG 
CCCGCCAATC CAACTGTGGC CACAACAATG CAAGAGCCAG CCATTACGGC GCCCAGTACT 
AATCCCAGCA GTTTGAAAAC GATGATCGCT CAAGCCGAAT ATGTGGGAAA ATCCCTCAAA 
GAAGTGGCCA GTTCACCGTT CAAGGATGAG TCCCAGGACC TGGTTCCAGT GGCCAAGCTG 
GTGGACAATG CCACCAGCCA GAACATGGGC TTCAACAGCT ATTTCCGGCC CAGCGATGTG 
GCCAATCACA TGGAGCAGAG CGACGAGGAG GGCCTCGTGG CCTCCGGAAG CGCCAGTGAG 
AGCAACAACA GCGGTACCGA GGACGTCACC AGCAGCAGTT CCAGCAGTGA GCCCAAGAAG 
AAGTCCGCCT ACAGCCTGGC GCCCAATCGT GTCTCCTGTC CGTATTGCCA GCGCATGTTC 
CCATGGAGCA GCTCGCTGCG CAGGCACATC CTCACCCACA CTGGCCAGAA GCCCTTCAAG 
TGCTCCCACT GCCCGCTGTT GTTCACCACC AAGAGCAACT GCGATCGCCA TCTCTTGCGC 
AAGCACGGCA ATGTGGAGTC GGCCATGTCC GTGTATGTGC CCACTGAGGA TGTGAGTGAA 
CCGATACCAG TGCCCAAGTC CGTCGAGGAG ATCGAGCTGG AGGAGCAGCG ACGGCGGCAG 
GAGGCAGAGC GCGAGAAGGA GCTGGAGCTG GAGCGCGAGC GGGAGTTGGA GCGAGAGCGG 
GAGCTGGAAA GGGAGCGCCA GCTGGAGAAG GAGAAGGAAC GCGAGCGCCA GCAGCTCATC 
CAAAAGCTGG CGGCTCAAAT GAATGCCGCT GCTACTGCTG CTGCCGTCGT TGCTGCAGCC 
TCAGCGGTAA ATGGTGGCGC ATCTGGTGGA CCCCATGGAC CCATTGCAGA TGCCTTGGCT 
GGCGGAGATC TGCCCTACAA GTGCCACCTG TGCGAGGGTT CCTTTGCCGA GCGTCTGCAG 
TGCCTGGAGC ATATCAAGCA GGCCCATGCC CACGAGTATG CCCTGCTCCT GGCCAAGGGA 
GCCATTGAAA CTGAGTCGCT GGAAGCCAAT CCTCACCAGC AGCCGTCGCA GCAGGCCGTG 
CATTCCGATG ACGAGGCTCC GAATGGCGGC GGTAACCGGG GCAAGTATCC CGACTACAGC 
AACCGCAAGG TTATCTGTGC CTTCTGCTTG CGTCGCTTTT GGTCAACGGA GGATCTGCGT 
CGCCACATGC GCACCCATTC GGGCGAGCGG CCATTCCAGT GTGATATCTG CCTGAGGAAG 
TTCACACTGA AGCACAGCAT GCTGCGGCAC ATGAAGAAGC ACAGTGGTAG GGCCCACAAT 
GGCGATACAC CGGGATCCGA TTGCTCCGAC GATGAGCAGG TGAGCAGCCC GCCCAGCACA 
CCACATCCCA CCCAGCCGAC CAGCGCCAAC AACAACAATA GCTGTCACAA TAACAATAAT 
AATGCCAACA ACAATAACAA CAATAACAAC AACAACAACA ACAACAACAG CAGCTCCAAG 
CTGGGACTAA AGCTCCACGA CCTGCTGGAC AAGGCGAGCG AATGGCGGGC CAGTCGCCTG 
GGTGAGCACA AGGAGAACAT GGGCGAGGCC ACGCCCTCGG GAGCAACGGT CGCTGGCTCG 
GATCTGATTG GCAATCTATT GGGCATCAGT GATCAGGGCA TTCTCAACAA GCTGCTCTCC 
TCCGCCGACG AGGCGGCCAA ACTTCTGGGT GTGGACAACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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peb cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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