CG3546 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG3546
Entrez Gene ID	31389
Full Name	CG3546 gene product from transcript CG3546-RA
Synonyms	Dmel\CG3546
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4C7-4C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_131944.2	NP_572172.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG3546 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG3546 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00785	NM_131944.2 Latest version!	Drosophila melanogaster uncharacterized protein (CG3546), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG3546 ( NM_131944.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572172.1 Drosophila melanogaster uncharacterized protein (CG3546), mRNA.

Price & Availability↑

CloneID	OFa00785
Clone ID Related Accession (Same CDS sequence)	NM_131944.2
Accession Version	NM_131944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_131944.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCCTGG TGGCCACCAG GGTCGCTGGA GAGACCATGA TCGAGGTGGA CGAAAATGGC 
GATGAGTACG AGCTGAAGCG ATTCTATATT CAGGGTCGCC AATTGAAGGG CGATGGCAGT 
CAGTCGCAGT GTGTGGTCAC GAGAAGGAAG CGTTCGGGTC GTGCAGCAGG ATCTGGATCA 
GGATCAGGAT CGGCAGCTCT GTCGGTTAGT TCGCAACCCC TGAAGGTGGA GAGTCTGCCG 
GCGAAGGAAC ATCGTGAGGG AGGAGCTGTT CTGCAGGAGG TGGAGAGCAA TAGCAATAGC 
AATAGACGTT ATGTCGCCCT CACTGGAGTC CACTTGGAGT CGCCTCTGCC CCAGGGTGAT 
GTGCAGAACG ATGACAACGA CGATGACGAC GAGGAGGACG ATGATGGCGA CGATCAGTCT 
TCCAGAAATC AGTCCAACTT TGTGCCCGCT GCCTTGGCTT TGCCCCTGCA AAGCCAGTCC 
ACTGCTGCCC ATCTGAATCA GGGAGTCAGT GTGGTCGCCA ATGCAGCCAG TCATGCCAAT 
GTGGCCGAGG ATGCTGGCAT CGTGGTGGTC CAATCGCATC AGCCGCCCAA AGTCAATCCC 
GATACCCATG CCGTCTTCGA GCTGAAGCCC GTTCAATCGG ATGTTGTTCA CCAGTCAGCC 
GGCGTTAGCT TCTATCCGTT CGTTCAGCCT TGGGAGGTGG TGGACCAGCA TGAGGATGAT 
GACTACGATG AGGACGACTA TGACGAAGAG GACTACGATG ATTTTTACTA CGGCGGCGGC 
AATGGCTACT ACAGAGATCA GCCCGGCTTT AATGGCCTGG GTGTTTTCGA GGAGGAGATC 
ATCAGCGAGG ACCAGCCCGT CTCTACGATA GCCAGTTGGG TAACCGGTGA TGATGACAGC 
GATATTCTTC CCGCTGTCAA TAAGCGGCCC AATCAGAAGA ACAAGAACAA GAAGAACCAG 
AAACAGAAAC AGAAACAGAA GAACAAGAAT CAGCAACAGA AGAACAAGCG ACAGCAGCAG 
AAGAAACGCA AGCAACAGGC TCAAGATGAC GAGCAGCCTG CGAAGCAAAA GAAAAAGCCG 
CAGCAGCAGG ATAAAGATCA GAAAAAGAAG CAGGATAAGG ATCAAAAGAA GAAGAAGCCA 
GAGGAGAATC CAAATCAGCA GGAGATGGAA CAGCTTACTC CCGTGGAAGT GATCAAGAAG 
CCAGTGGCTA TGGAACAATC AGCCGTTGCT TCTTCGGCCA CAAGTAGCAG CAATGTCGCT 
GCATCCTCCA GCAGTGTTTC ATCATCGGGT AGCAATTCTT CGGACAAGAA GAAGAACAAG 
AAGAAGAAGA AGAAGCCTAG CAGCAGCAGC AGCAGCAGTC TTAGCCAGCG CAGGACCAAG 
AAGAAGAAGA AGCAGTCGAG CAGCAGCAGC AGCAGCAGCA GCAGCATCAT CAGCAGCAGC 
AACAAGCGTC GCCGACCCAG CAGCAGTTCC TCCATTGGTG GCTACCGTCG CCGTCGTCCG 
CAGCAGCAGC AGTTGCAGCA GCAACGTCGC CGCAAAACGC AACAGCAGCA AAAGCGCCGT 
CGCCAGCAGC AACAGCAGAA GAGACGCCGC CAGCAGCAGC AACAACAACG CCGCCGATTG 
CGCAACAAGA ATCGTCAATT CTACGGCGAC GAGCCCATCA TCAACTGCAT TTACATCAAC 
AAGGATCCAG TGACGACCAC CACCACGCCG CGTCCCTTCT GGAATATCCT GGGTCGCCAA 
TCGGATTCGA AGCCCAGTGC GGAATCCACT GTTGTCTCTT CCGAAGAATC CCAAACGGAT 
CCCGTCGCCC AGCAGGCGGT TCTTCAGGCC GTTCGTCGCA ACTTTGGCGA ATTCGGCGGA 
CGAAAGCGCA CCAATTTGCG TTTCGTGTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572172.1
CDS	39..1931
Translation	MCLVATRVAGETMIEVDENGDEYELKRFYIQGRQLKGDGSQSQCVVTRRKRSGRAAGSGSGSGSAALSVSSQPLKVESLPAKEHREGGAVLQEVESNSNSNRRYVALTGVHLESPLPQGDVQNDDNDDDDEEDDDGDDQSSRNQSNFVPAALALPLQSQSTAAHLNQGVSVVANAASHANVAEDAGIVVVQSHQPPKVNPDTHAVFELKPVQSDVVHQSAGVSFYPFVQPWEVVDQHEDDDYDEDDYDEEDYDDFYYGGGNGYYRDQPGFNGLGVFEEEIISEDQPVSTIASWVTGDDDSDILPAVNKRPNQKNKNKKNQKQKQKQKNKNQQQKNKRQQQKKRKQQAQDDEQPAKQKKKPQQQDKDQKKKQDKDQKKKKPEENPNQQEMEQLTPVEVIKKPVAMEQSAVASSATSSSNVAASSSSVSSSGSNSSDKKKNKKKKKKPSSSSSSSLSQRRTKKKKKQSSSSSSSSSSIISSSNKRRRPSSSSSIGGYRRRRPQQQQLQQQRRRKTQQQQKRRRQQQQQKRRRQQQQQQRRRLRNKNRQFYGDEPIINCIYINKDPVTTTTTPRPFWNILGRQSDSKPSAESTVVSSEESQTDPVAQQAVLQAVRRNFGEFGGRKRTNLRFVS

Target ORF information:

RefSeq Version	NM_131944.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG3546), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131944.2

ORF Insert Sequence:

ATGTGCCTGG TGGCCACCAG GGTCGCTGGA GAGACCATGA TCGAGGTGGA CGAAAATGGC 
GATGAGTACG AGCTGAAGCG ATTCTATATT CAGGGTCGCC AATTGAAGGG CGATGGCAGT 
CAGTCGCAGT GTGTGGTCAC GAGAAGGAAG CGTTCGGGTC GTGCAGCAGG ATCTGGATCA 
GGATCAGGAT CGGCAGCTCT GTCGGTTAGT TCGCAACCCC TGAAGGTGGA GAGTCTGCCG 
GCGAAGGAAC ATCGTGAGGG AGGAGCTGTT CTGCAGGAGG TGGAGAGCAA TAGCAATAGC 
AATAGACGTT ATGTCGCCCT CACTGGAGTC CACTTGGAGT CGCCTCTGCC CCAGGGTGAT 
GTGCAGAACG ATGACAACGA CGATGACGAC GAGGAGGACG ATGATGGCGA CGATCAGTCT 
TCCAGAAATC AGTCCAACTT TGTGCCCGCT GCCTTGGCTT TGCCCCTGCA AAGCCAGTCC 
ACTGCTGCCC ATCTGAATCA GGGAGTCAGT GTGGTCGCCA ATGCAGCCAG TCATGCCAAT 
GTGGCCGAGG ATGCTGGCAT CGTGGTGGTC CAATCGCATC AGCCGCCCAA AGTCAATCCC 
GATACCCATG CCGTCTTCGA GCTGAAGCCC GTTCAATCGG ATGTTGTTCA CCAGTCAGCC 
GGCGTTAGCT TCTATCCGTT CGTTCAGCCT TGGGAGGTGG TGGACCAGCA TGAGGATGAT 
GACTACGATG AGGACGACTA TGACGAAGAG GACTACGATG ATTTTTACTA CGGCGGCGGC 
AATGGCTACT ACAGAGATCA GCCCGGCTTT AATGGCCTGG GTGTTTTCGA GGAGGAGATC 
ATCAGCGAGG ACCAGCCCGT CTCTACGATA GCCAGTTGGG TAACCGGTGA TGATGACAGC 
GATATTCTTC CCGCTGTCAA TAAGCGGCCC AATCAGAAGA ACAAGAACAA GAAGAACCAG 
AAACAGAAAC AGAAACAGAA GAACAAGAAT CAGCAACAGA AGAACAAGCG ACAGCAGCAG 
AAGAAACGCA AGCAACAGGC TCAAGATGAC GAGCAGCCTG CGAAGCAAAA GAAAAAGCCG 
CAGCAGCAGG ATAAAGATCA GAAAAAGAAG CAGGATAAGG ATCAAAAGAA GAAGAAGCCA 
GAGGAGAATC CAAATCAGCA GGAGATGGAA CAGCTTACTC CCGTGGAAGT GATCAAGAAG 
CCAGTGGCTA TGGAACAATC AGCCGTTGCT TCTTCGGCCA CAAGTAGCAG CAATGTCGCT 
GCATCCTCCA GCAGTGTTTC ATCATCGGGT AGCAATTCTT CGGACAAGAA GAAGAACAAG 
AAGAAGAAGA AGAAGCCTAG CAGCAGCAGC AGCAGCAGTC TTAGCCAGCG CAGGACCAAG 
AAGAAGAAGA AGCAGTCGAG CAGCAGCAGC AGCAGCAGCA GCAGCATCAT CAGCAGCAGC 
AACAAGCGTC GCCGACCCAG CAGCAGTTCC TCCATTGGTG GCTACCGTCG CCGTCGTCCG 
CAGCAGCAGC AGTTGCAGCA GCAACGTCGC CGCAAAACGC AACAGCAGCA AAAGCGCCGT 
CGCCAGCAGC AACAGCAGAA GAGACGCCGC CAGCAGCAGC AACAACAACG CCGCCGATTG 
CGCAACAAGA ATCGTCAATT CTACGGCGAC GAGCCCATCA TCAACTGCAT TTACATCAAC 
AAGGATCCAG TGACGACCAC CACCACGCCG CGTCCCTTCT GGAATATCCT GGGTCGCCAA 
TCGGATTCGA AGCCCAGTGC GGAATCCACT GTTGTCTCTT CCGAAGAATC CCAAACGGAT 
CCCGTCGCCC AGCAGGCGGT TCTTCAGGCC GTTCGTCGCA ACTTTGGCGA ATTCGGCGGA 
CGAAAGCGCA CCAATTTGCG TTTCGTGTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG3546 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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