mei-9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mei-9
Entrez Gene ID	31373
Full Name	meiotic 9
Synonyms	CG3697,DhR1,DmXPF,Dmel\CG3697,MEI-9,Mei-9,Rad1p,mei9,mus(1)110,mus110,xpf/mei-9
General protein information	Preferred Names meiotic 9 Names CG3697 gene product from transcript CG3697-RA CG3697-PA CG3697-PB mei-9-PA mei-9-PB meiotic-9
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4B6-4B6\|1-6.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080329.3	NP_525068.1	meiotic 9, isoform A
NM_001297930.1	NP_001284859.1	meiotic 9, isoform B

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	RAD1	NP_015303.1
Arabidopsis thaliana (thale cress)	UVH1	NP_198931.1
Gallus gallus (chicken)	ERCC4	XP_414734.3
Drosophila melanogaster (fruit fly)	mei-9	NP_525068.1
Magnaporthe oryzae (rice blast fungus)	MGG_02804	XP_003720937.1
Neurospora crassa	NCU07440	XP_964627.2
Bos taurus (cattle)	ERCC4	NP_001192289.1
Mus musculus (house mouse)	Ercc4	NP_056584.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002805	XP_563334.4
Oryza sativa (rice)	Os03g0100400	NP_001048645.1
Xenopus tropicalis (tropical clawed frog)	ercc4	XP_002935520.2
Pan troglodytes (chimpanzee)	ERCC4	XP_510831.2
	ERCC4	XP_001107209.1
Rattus norvegicus (Norway rat)	Ercc4	XP_006245762.1
Schizosaccharomyces pombe (fission yeast)	rad16	NP_587855.2
Homo sapiens (human)	ERCC4	NP_005227.1
Canis lupus familiaris (dog)	ERCC4	XP_536967.3
Rattus norvegicus (Norway rat)	Ercc4l1	XP_003750828.1
Danio rerio (zebrafish)	ercc4	NP_956079.1
Kluyveromyces lactis	KLLA0D12210g	XP_453606.1
Eremothecium gossypii	AGOS_AFR456C	NP_986003.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HDM, MEI-9, MUS312, and ERCC1 form a complex that resolves meiotic recombination intermediates into crossovers.
Title: Drosophila hold'em is required for a subset of meiotic crossovers and interacts with the dna repair endonuclease complex subunits MEI-9 and ERCC1.

These results support a model in which MEI-9 nicks Holliday junctions to generate crossovers during meiotic recombination, and, in the absence of MEI-9 activity, the double Holliday junction intermediate instead undergoes dissolution.
Title: Heteroduplex DNA in meiotic recombination in Drosophila mei-9 mutants.

Drosophila mei-9 has roles in DNA repair and recombination, as shown by mutational analysis
Title: Mutational analysis of the Drosophila DNA repair and recombination gene mei-9.

MUS312 facilitates resolution of meiotic Holliday junction intermediates by MEI-9
Title: Drosophila MUS312 interacts with the nucleotide excision repair endonuclease MEI-9 to generate meiotic crossovers.

The following mei-9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mei-9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00768	NM_080329.3 Latest version!	Drosophila melanogaster meiotic 9 (mei-9), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa00768	NM_001297930.1 Latest version!	Drosophila melanogaster meiotic 9 (mei-9), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mei-9 ( NM_080329.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525068.1 Drosophila melanogaster meiotic 9 (mei-9), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00768
Clone ID Related Accession (Same CDS sequence)	NM_080329.3 , NM_001297930.1
Accession Version	NM_080329.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiotic 9, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 26, 2009 this sequence version replaced NM_080329.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGATT CGTGCGCGGA AAATGCCGCC AAAGGGACGG AAAATGAGCG GCCGAAGGAA 
GTGGAGGCCA GCGCCGACAC CGTCCCACAG GTGGAGGAGG GCGTAGAGGA GTATTTGAAG 
CGTAAGAACA TGGTCCTCCT CGACTACGAA AAGCAAATGT TTCTCGATTT GGTCGAGGCC 
GATGGTCTGC TGGTTTGCGC CAAGGGCCTG AGCTACGACC GCGTTGTAAT CAGCATCCTC 
AAGGCCTACA GCGATTCCGG CAATCTTGTG CTTGTGATCA ACAGTTCTGA CTGGGAGGAG 
CAGTATTACA AGTCCAAAAT CGAACCCAAA TATGTCCACG AAGTGGCCAG CACGGCCACC 
GAAAGAGAGC GCGTTTATCT GGAAGGCGGG CTGCAGTTCA TTAGCACGCG CATCCTGGTG 
GTGGATCTGC TGAAGCAGCG AATCCCCATC GAGCTGATAA GCGGAATCAT TGTCCTGCGG 
GCGCACACCA TAATTGAGAG TTGCCAGGAG GCGTTCGCCT TGCGATTGTT CCGTCAGAAG 
AATAAAACCG GATTCGTGAA GGCTTTCTCC AGCAGCCCGG AGGCCTTCAC CATTGGCTAC 
TCACATGTGG AGCGGACCAT GCGCAATCTG TTCGTAAAGC ATTTGTATAT CTGGCCCCGT 
TTTCATGAGA GCGTGCGCAC CGTCCTGCAG CCATGGAAGA TACAATCAAT CGAGATGCAT 
GTTCCAATTA GCCAGAACAT AACGTCCATT CAATCACACA TTCTGGAGAT AATGAACTTT 
CTCGTGCAGG AAATCAAGCG GATAAATCGT ACTGTCGATA TGGAGGCCGT TACCGTGGAG 
AACTGTGTGA CCAAATCATT CCACAAGATC CTGCAGGCGC AACTGGATTG CATCTGGCAT 
CAGCTTAATT CTCAAACAAA GTTGATAGTA GCAGATCTTA AGATTCTGCG CAGCTTAATG 
ATCTCCACCA TGTACCACGA TGCTGTGAGC GCATATGCCT TTATGAAACG CTACCGGAGC 
ACGGAGTACG CGTTGAGCAA CTCAGGATGG ACTTTACTGG ACGCCGCCGA ACAGATCTTT 
AAACTATCCA GGCAGCGTGT TTTCAATGGC CAGCAGGAGT TCGAACCGGA ACCCTGTCCC 
AAATGGCAAA CTCTTACGGA TTTGCTTACC AAGGAAATAC CCGGCGATAT GCGGCGGAGT 
AGGCGATCGG AACAACAACC GAAAGTGCTG ATCCTCTGCC AGGATGCACG GACGTGTCAT 
CAGTTGAAAC AGTATCTCAC GCAAGGTGGG CCACGTTTTC TACTCCAGCA GGCATTGCAA 
CATGAAGTGC CGGTGGGCAA ACTCAGTGAC AATTACGCCA AAGAGAGCCA AACTCGTTCA 
GCTCCGCCAA AGAATGTTTC ATCCAATAAG GAGCTGCGTA GGGAAGAGGT TTCTGGTTCT 
CAGCCACCTT TAGCTGGTAT GGATGAATTG GCCCAGCTTT TGAGCGAATC GGAAACAGAG 
GGTCAGCACT TCGAAGAGAG TTACATGCTC ACCATGACGC AGCCTGTGGA AGTCGGGCCA 
GCAGCGATAG ACATTAAACC CGATCCGGAT GTGTCTATTT TCGAGACAAT ACCGGAACTA 
GAACAATTTG ATGTCACTGC GGCCCTGGCC TCTGTGCCAC ATCAGCCATA CATTTGCTTG 
CAGACCTTCA AAACGGAGAG GGAGGGCTCC ATGGCACTGG AGCACATGCT GGAGCAACTG 
CAGCCGCACT ATGTTGTCAT GTACAACATG AATGTGACAG CCATTCGGCA ATTGGAGGTT 
TTCGAGGCCA GACGTCGCCT GCCGCCTGCA GATCGCATGA AGGTGTATTT CCTGATCCAT 
GCACGCACAG TGGAGGAGCA AGCCTATCTG ACCAGTTTGC GTCGAGAAAA GGCAGCCTTC 
GAGTTCATTA TCGACACTAA AAGTAAAATG GTGATTCCAA AGTACCAGGA TGGAAAGACC 
GATGAAGCGT TTTTGTTATT AAAGACTTAC GATGATGAGC CGACGGACGA GAATGCCAAG 
AGTCGCCAAG CGGGTGGGCA AGCTCCGCAG GCGACCAAAG AGACGCCAAA AGTGATTGTG 
GATATGCGTG AGTTTCGCTC AGATCTGCCC TGTTTGATTC ACAAGCGTGG TCTGGAAGTG 
CTGCCCTTGA CCATTACGAT TGGCGATTAT ATACTCACAC CGGATATTTG TGTGGAGCGA 
AAGTCCATTT CTGATTTGAT TGGGTCTCTG AATTCGGGCA GATTGTACAA CCAATGCGTG 
CAAATGCAGC GGCATTATGC AAAGCCCATC CTTTTGATTG AGTTCGATCA GAATAAGCCC 
TTTCACCTGC AGGGCAAATT TATGTTATCG CAGCAGACCA GCATGGCCAA TGCAGATATT 
GTGCAGAAAC TGCAACTTCT TACGTTACAT TTTCCCAAGC TGCGACTCAT TTGGTCACCC 
AGTCCGTATG CCACTGCCCA GCTATTCGAG GAGCTGAAGC TGGGTAAACC GGAACCGGAT 
CCTCAAACGG CGGCCGCCTT GGGCAGCGAC GAGCCAACGG CGGGTGAGCA ATTGCATTTC 
AACAGCGGCA TCTACGATTT CCTGCTACGT CTGCCCGGCG TTCATACGCG CAATATACAT 
GGCCTACTCC GGAAGGGCGG CAGCCTGCGG CAATTGTTGC TGCGCAGCCA GAAGGAACTG 
GAGGAGCTGC TGCAGTCGCA GGAGAGCGCC AAGCTGCTGT ACGATATCCT GCATGTGGCC 
CATTTGCCGG AGAAGGACGA GGTTACCGGC TCGACGGCGC TGCTGGCGGC TAGCAAGCAA 
TTTGGCGCCG GATCGCACAA TCGCTTCAGG ATGGCGGCAG CCGCATCGCG AAGGGGTCGC 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525068.1
CDS	188..3073
Translation	MADSCAENAAKGTENERPKEVEASADTVPQVEEGVEEYLKRKNMVLLDYEKQMFLDLVEADGLLVCAKGLSYDRVVISILKAYSDSGNLVLVINSSDWEEQYYKSKIEPKYVHEVASTATERERVYLEGGLQFISTRILVVDLLKQRIPIELISGIIVLRAHTIIESCQEAFALRLFRQKNKTGFVKAFSSSPEAFTIGYSHVERTMRNLFVKHLYIWPRFHESVRTVLQPWKIQSIEMHVPISQNITSIQSHILEIMNFLVQEIKRINRTVDMEAVTVENCVTKSFHKILQAQLDCIWHQLNSQTKLIVADLKILRSLMISTMYHDAVSAYAFMKRYRSTEYALSNSGWTLLDAAEQIFKLSRQRVFNGQQEFEPEPCPKWQTLTDLLTKEIPGDMRRSRRSEQQPKVLILCQDARTCHQLKQYLTQGGPRFLLQQALQHEVPVGKLSDNYAKESQTRSAPPKNVSSNKELRREEVSGSQPPLAGMDELAQLLSESETEGQHFEESYMLTMTQPVEVGPAAIDIKPDPDVSIFETIPELEQFDVTAALASVPHQPYICLQTFKTEREGSMALEHMLEQLQPHYVVMYNMNVTAIRQLEVFEARRRLPPADRMKVYFLIHARTVEEQAYLTSLRREKAAFEFIIDTKSKMVIPKYQDGKTDEAFLLLKTYDDEPTDENAKSRQAGGQAPQATKETPKVIVDMREFRSDLPCLIHKRGLEVLPLTITIGDYILTPDICVERKSISDLIGSLNSGRLYNQCVQMQRHYAKPILLIEFDQNKPFHLQGKFMLSQQTSMANADIVQKLQLLTLHFPKLRLIWSPSPYATAQLFEELKLGKPEPDPQTAAALGSDEPTAGEQLHFNSGIYDFLLRLPGVHTRNIHGLLRKGGSLRQLLLRSQKELEELLQSQESAKLLYDILHVAHLPEKDEVTGSTALLAASKQFGAGSHNRFRMAAAASRRGRR

Target ORF information:

RefSeq Version	NM_080329.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiotic 9 (mei-9), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080329.3

ORF Insert Sequence:

ATGGCCGATT CGTGCGCGGA AAATGCCGCC AAAGGGACGG AAAATGAGCG GCCGAAGGAA 
GTGGAGGCCA GCGCCGACAC CGTCCCACAG GTGGAGGAGG GCGTAGAGGA GTATTTGAAG 
CGTAAGAACA TGGTCCTCCT CGACTACGAA AAGCAAATGT TTCTCGATTT GGTCGAGGCC 
GATGGTCTGC TGGTTTGCGC CAAGGGCCTG AGCTACGACC GCGTTGTAAT CAGCATCCTC 
AAGGCCTACA GCGATTCCGG CAATCTTGTG CTTGTGATCA ACAGTTCTGA CTGGGAGGAG 
CAGTATTACA AGTCCAAAAT CGAACCCAAA TATGTCCACG AAGTGGCCAG CACGGCCACC 
GAAAGAGAGC GCGTTTATCT GGAAGGCGGG CTGCAGTTCA TTAGCACGCG CATCCTGGTG 
GTGGATCTGC TGAAGCAGCG AATCCCCATC GAGCTGATAA GCGGAATCAT TGTCCTGCGG 
GCGCACACCA TAATTGAGAG TTGCCAGGAG GCGTTCGCCT TGCGATTGTT CCGTCAGAAG 
AATAAAACCG GATTCGTGAA GGCTTTCTCC AGCAGCCCGG AGGCCTTCAC CATTGGCTAC 
TCACATGTGG AGCGGACCAT GCGCAATCTG TTCGTAAAGC ATTTGTATAT CTGGCCCCGT 
TTTCATGAGA GCGTGCGCAC CGTCCTGCAG CCATGGAAGA TACAATCAAT CGAGATGCAT 
GTTCCAATTA GCCAGAACAT AACGTCCATT CAATCACACA TTCTGGAGAT AATGAACTTT 
CTCGTGCAGG AAATCAAGCG GATAAATCGT ACTGTCGATA TGGAGGCCGT TACCGTGGAG 
AACTGTGTGA CCAAATCATT CCACAAGATC CTGCAGGCGC AACTGGATTG CATCTGGCAT 
CAGCTTAATT CTCAAACAAA GTTGATAGTA GCAGATCTTA AGATTCTGCG CAGCTTAATG 
ATCTCCACCA TGTACCACGA TGCTGTGAGC GCATATGCCT TTATGAAACG CTACCGGAGC 
ACGGAGTACG CGTTGAGCAA CTCAGGATGG ACTTTACTGG ACGCCGCCGA ACAGATCTTT 
AAACTATCCA GGCAGCGTGT TTTCAATGGC CAGCAGGAGT TCGAACCGGA ACCCTGTCCC 
AAATGGCAAA CTCTTACGGA TTTGCTTACC AAGGAAATAC CCGGCGATAT GCGGCGGAGT 
AGGCGATCGG AACAACAACC GAAAGTGCTG ATCCTCTGCC AGGATGCACG GACGTGTCAT 
CAGTTGAAAC AGTATCTCAC GCAAGGTGGG CCACGTTTTC TACTCCAGCA GGCATTGCAA 
CATGAAGTGC CGGTGGGCAA ACTCAGTGAC AATTACGCCA AAGAGAGCCA AACTCGTTCA 
GCTCCGCCAA AGAATGTTTC ATCCAATAAG GAGCTGCGTA GGGAAGAGGT TTCTGGTTCT 
CAGCCACCTT TAGCTGGTAT GGATGAATTG GCCCAGCTTT TGAGCGAATC GGAAACAGAG 
GGTCAGCACT TCGAAGAGAG TTACATGCTC ACCATGACGC AGCCTGTGGA AGTCGGGCCA 
GCAGCGATAG ACATTAAACC CGATCCGGAT GTGTCTATTT TCGAGACAAT ACCGGAACTA 
GAACAATTTG ATGTCACTGC GGCCCTGGCC TCTGTGCCAC ATCAGCCATA CATTTGCTTG 
CAGACCTTCA AAACGGAGAG GGAGGGCTCC ATGGCACTGG AGCACATGCT GGAGCAACTG 
CAGCCGCACT ATGTTGTCAT GTACAACATG AATGTGACAG CCATTCGGCA ATTGGAGGTT 
TTCGAGGCCA GACGTCGCCT GCCGCCTGCA GATCGCATGA AGGTGTATTT CCTGATCCAT 
GCACGCACAG TGGAGGAGCA AGCCTATCTG ACCAGTTTGC GTCGAGAAAA GGCAGCCTTC 
GAGTTCATTA TCGACACTAA AAGTAAAATG GTGATTCCAA AGTACCAGGA TGGAAAGACC 
GATGAAGCGT TTTTGTTATT AAAGACTTAC GATGATGAGC CGACGGACGA GAATGCCAAG 
AGTCGCCAAG CGGGTGGGCA AGCTCCGCAG GCGACCAAAG AGACGCCAAA AGTGATTGTG 
GATATGCGTG AGTTTCGCTC AGATCTGCCC TGTTTGATTC ACAAGCGTGG TCTGGAAGTG 
CTGCCCTTGA CCATTACGAT TGGCGATTAT ATACTCACAC CGGATATTTG TGTGGAGCGA 
AAGTCCATTT CTGATTTGAT TGGGTCTCTG AATTCGGGCA GATTGTACAA CCAATGCGTG 
CAAATGCAGC GGCATTATGC AAAGCCCATC CTTTTGATTG AGTTCGATCA GAATAAGCCC 
TTTCACCTGC AGGGCAAATT TATGTTATCG CAGCAGACCA GCATGGCCAA TGCAGATATT 
GTGCAGAAAC TGCAACTTCT TACGTTACAT TTTCCCAAGC TGCGACTCAT TTGGTCACCC 
AGTCCGTATG CCACTGCCCA GCTATTCGAG GAGCTGAAGC TGGGTAAACC GGAACCGGAT 
CCTCAAACGG CGGCCGCCTT GGGCAGCGAC GAGCCAACGG CGGGTGAGCA ATTGCATTTC 
AACAGCGGCA TCTACGATTT CCTGCTACGT CTGCCCGGCG TTCATACGCG CAATATACAT 
GGCCTACTCC GGAAGGGCGG CAGCCTGCGG CAATTGTTGC TGCGCAGCCA GAAGGAACTG 
GAGGAGCTGC TGCAGTCGCA GGAGAGCGCC AAGCTGCTGT ACGATATCCT GCATGTGGCC 
CATTTGCCGG AGAAGGACGA GGTTACCGGC TCGACGGCGC TGCTGGCGGC TAGCAAGCAA 
TTTGGCGCCG GATCGCACAA TCGCTTCAGG ATGGCGGCAG CCGCATCGCG AAGGGGTCGC 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mei-9 ( NM_001297930.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284859.1 Drosophila melanogaster meiotic 9 (mei-9), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00768
Clone ID Related Accession (Same CDS sequence)	NM_080329.3 , NM_001297930.1
Accession Version	NM_001297930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiotic 9, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGATT CGTGCGCGGA AAATGCCGCC AAAGGGACGG AAAATGAGCG GCCGAAGGAA 
GTGGAGGCCA GCGCCGACAC CGTCCCACAG GTGGAGGAGG GCGTAGAGGA GTATTTGAAG 
CGTAAGAACA TGGTCCTCCT CGACTACGAA AAGCAAATGT TTCTCGATTT GGTCGAGGCC 
GATGGTCTGC TGGTTTGCGC CAAGGGCCTG AGCTACGACC GCGTTGTAAT CAGCATCCTC 
AAGGCCTACA GCGATTCCGG CAATCTTGTG CTTGTGATCA ACAGTTCTGA CTGGGAGGAG 
CAGTATTACA AGTCCAAAAT CGAACCCAAA TATGTCCACG AAGTGGCCAG CACGGCCACC 
GAAAGAGAGC GCGTTTATCT GGAAGGCGGG CTGCAGTTCA TTAGCACGCG CATCCTGGTG 
GTGGATCTGC TGAAGCAGCG AATCCCCATC GAGCTGATAA GCGGAATCAT TGTCCTGCGG 
GCGCACACCA TAATTGAGAG TTGCCAGGAG GCGTTCGCCT TGCGATTGTT CCGTCAGAAG 
AATAAAACCG GATTCGTGAA GGCTTTCTCC AGCAGCCCGG AGGCCTTCAC CATTGGCTAC 
TCACATGTGG AGCGGACCAT GCGCAATCTG TTCGTAAAGC ATTTGTATAT CTGGCCCCGT 
TTTCATGAGA GCGTGCGCAC CGTCCTGCAG CCATGGAAGA TACAATCAAT CGAGATGCAT 
GTTCCAATTA GCCAGAACAT AACGTCCATT CAATCACACA TTCTGGAGAT AATGAACTTT 
CTCGTGCAGG AAATCAAGCG GATAAATCGT ACTGTCGATA TGGAGGCCGT TACCGTGGAG 
AACTGTGTGA CCAAATCATT CCACAAGATC CTGCAGGCGC AACTGGATTG CATCTGGCAT 
CAGCTTAATT CTCAAACAAA GTTGATAGTA GCAGATCTTA AGATTCTGCG CAGCTTAATG 
ATCTCCACCA TGTACCACGA TGCTGTGAGC GCATATGCCT TTATGAAACG CTACCGGAGC 
ACGGAGTACG CGTTGAGCAA CTCAGGATGG ACTTTACTGG ACGCCGCCGA ACAGATCTTT 
AAACTATCCA GGCAGCGTGT TTTCAATGGC CAGCAGGAGT TCGAACCGGA ACCCTGTCCC 
AAATGGCAAA CTCTTACGGA TTTGCTTACC AAGGAAATAC CCGGCGATAT GCGGCGGAGT 
AGGCGATCGG AACAACAACC GAAAGTGCTG ATCCTCTGCC AGGATGCACG GACGTGTCAT 
CAGTTGAAAC AGTATCTCAC GCAAGGTGGG CCACGTTTTC TACTCCAGCA GGCATTGCAA 
CATGAAGTGC CGGTGGGCAA ACTCAGTGAC AATTACGCCA AAGAGAGCCA AACTCGTTCA 
GCTCCGCCAA AGAATGTTTC ATCCAATAAG GAGCTGCGTA GGGAAGAGGT TTCTGGTTCT 
CAGCCACCTT TAGCTGGTAT GGATGAATTG GCCCAGCTTT TGAGCGAATC GGAAACAGAG 
GGTCAGCACT TCGAAGAGAG TTACATGCTC ACCATGACGC AGCCTGTGGA AGTCGGGCCA 
GCAGCGATAG ACATTAAACC CGATCCGGAT GTGTCTATTT TCGAGACAAT ACCGGAACTA 
GAACAATTTG ATGTCACTGC GGCCCTGGCC TCTGTGCCAC ATCAGCCATA CATTTGCTTG 
CAGACCTTCA AAACGGAGAG GGAGGGCTCC ATGGCACTGG AGCACATGCT GGAGCAACTG 
CAGCCGCACT ATGTTGTCAT GTACAACATG AATGTGACAG CCATTCGGCA ATTGGAGGTT 
TTCGAGGCCA GACGTCGCCT GCCGCCTGCA GATCGCATGA AGGTGTATTT CCTGATCCAT 
GCACGCACAG TGGAGGAGCA AGCCTATCTG ACCAGTTTGC GTCGAGAAAA GGCAGCCTTC 
GAGTTCATTA TCGACACTAA AAGTAAAATG GTGATTCCAA AGTACCAGGA TGGAAAGACC 
GATGAAGCGT TTTTGTTATT AAAGACTTAC GATGATGAGC CGACGGACGA GAATGCCAAG 
AGTCGCCAAG CGGGTGGGCA AGCTCCGCAG GCGACCAAAG AGACGCCAAA AGTGATTGTG 
GATATGCGTG AGTTTCGCTC AGATCTGCCC TGTTTGATTC ACAAGCGTGG TCTGGAAGTG 
CTGCCCTTGA CCATTACGAT TGGCGATTAT ATACTCACAC CGGATATTTG TGTGGAGCGA 
AAGTCCATTT CTGATTTGAT TGGGTCTCTG AATTCGGGCA GATTGTACAA CCAATGCGTG 
CAAATGCAGC GGCATTATGC AAAGCCCATC CTTTTGATTG AGTTCGATCA GAATAAGCCC 
TTTCACCTGC AGGGCAAATT TATGTTATCG CAGCAGACCA GCATGGCCAA TGCAGATATT 
GTGCAGAAAC TGCAACTTCT TACGTTACAT TTTCCCAAGC TGCGACTCAT TTGGTCACCC 
AGTCCGTATG CCACTGCCCA GCTATTCGAG GAGCTGAAGC TGGGTAAACC GGAACCGGAT 
CCTCAAACGG CGGCCGCCTT GGGCAGCGAC GAGCCAACGG CGGGTGAGCA ATTGCATTTC 
AACAGCGGCA TCTACGATTT CCTGCTACGT CTGCCCGGCG TTCATACGCG CAATATACAT 
GGCCTACTCC GGAAGGGCGG CAGCCTGCGG CAATTGTTGC TGCGCAGCCA GAAGGAACTG 
GAGGAGCTGC TGCAGTCGCA GGAGAGCGCC AAGCTGCTGT ACGATATCCT GCATGTGGCC 
CATTTGCCGG AGAAGGACGA GGTTACCGGC TCGACGGCGC TGCTGGCGGC TAGCAAGCAA 
TTTGGCGCCG GATCGCACAA TCGCTTCAGG ATGGCGGCAG CCGCATCGCG AAGGGGTCGC 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284859.1
CDS	73..2958
Translation	MADSCAENAAKGTENERPKEVEASADTVPQVEEGVEEYLKRKNMVLLDYEKQMFLDLVEADGLLVCAKGLSYDRVVISILKAYSDSGNLVLVINSSDWEEQYYKSKIEPKYVHEVASTATERERVYLEGGLQFISTRILVVDLLKQRIPIELISGIIVLRAHTIIESCQEAFALRLFRQKNKTGFVKAFSSSPEAFTIGYSHVERTMRNLFVKHLYIWPRFHESVRTVLQPWKIQSIEMHVPISQNITSIQSHILEIMNFLVQEIKRINRTVDMEAVTVENCVTKSFHKILQAQLDCIWHQLNSQTKLIVADLKILRSLMISTMYHDAVSAYAFMKRYRSTEYALSNSGWTLLDAAEQIFKLSRQRVFNGQQEFEPEPCPKWQTLTDLLTKEIPGDMRRSRRSEQQPKVLILCQDARTCHQLKQYLTQGGPRFLLQQALQHEVPVGKLSDNYAKESQTRSAPPKNVSSNKELRREEVSGSQPPLAGMDELAQLLSESETEGQHFEESYMLTMTQPVEVGPAAIDIKPDPDVSIFETIPELEQFDVTAALASVPHQPYICLQTFKTEREGSMALEHMLEQLQPHYVVMYNMNVTAIRQLEVFEARRRLPPADRMKVYFLIHARTVEEQAYLTSLRREKAAFEFIIDTKSKMVIPKYQDGKTDEAFLLLKTYDDEPTDENAKSRQAGGQAPQATKETPKVIVDMREFRSDLPCLIHKRGLEVLPLTITIGDYILTPDICVERKSISDLIGSLNSGRLYNQCVQMQRHYAKPILLIEFDQNKPFHLQGKFMLSQQTSMANADIVQKLQLLTLHFPKLRLIWSPSPYATAQLFEELKLGKPEPDPQTAAALGSDEPTAGEQLHFNSGIYDFLLRLPGVHTRNIHGLLRKGGSLRQLLLRSQKELEELLQSQESAKLLYDILHVAHLPEKDEVTGSTALLAASKQFGAGSHNRFRMAAAASRRGRR

Target ORF information:

RefSeq Version	NM_001297930.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiotic 9 (mei-9), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297930.1

ORF Insert Sequence:

ATGGCCGATT CGTGCGCGGA AAATGCCGCC AAAGGGACGG AAAATGAGCG GCCGAAGGAA 
GTGGAGGCCA GCGCCGACAC CGTCCCACAG GTGGAGGAGG GCGTAGAGGA GTATTTGAAG 
CGTAAGAACA TGGTCCTCCT CGACTACGAA AAGCAAATGT TTCTCGATTT GGTCGAGGCC 
GATGGTCTGC TGGTTTGCGC CAAGGGCCTG AGCTACGACC GCGTTGTAAT CAGCATCCTC 
AAGGCCTACA GCGATTCCGG CAATCTTGTG CTTGTGATCA ACAGTTCTGA CTGGGAGGAG 
CAGTATTACA AGTCCAAAAT CGAACCCAAA TATGTCCACG AAGTGGCCAG CACGGCCACC 
GAAAGAGAGC GCGTTTATCT GGAAGGCGGG CTGCAGTTCA TTAGCACGCG CATCCTGGTG 
GTGGATCTGC TGAAGCAGCG AATCCCCATC GAGCTGATAA GCGGAATCAT TGTCCTGCGG 
GCGCACACCA TAATTGAGAG TTGCCAGGAG GCGTTCGCCT TGCGATTGTT CCGTCAGAAG 
AATAAAACCG GATTCGTGAA GGCTTTCTCC AGCAGCCCGG AGGCCTTCAC CATTGGCTAC 
TCACATGTGG AGCGGACCAT GCGCAATCTG TTCGTAAAGC ATTTGTATAT CTGGCCCCGT 
TTTCATGAGA GCGTGCGCAC CGTCCTGCAG CCATGGAAGA TACAATCAAT CGAGATGCAT 
GTTCCAATTA GCCAGAACAT AACGTCCATT CAATCACACA TTCTGGAGAT AATGAACTTT 
CTCGTGCAGG AAATCAAGCG GATAAATCGT ACTGTCGATA TGGAGGCCGT TACCGTGGAG 
AACTGTGTGA CCAAATCATT CCACAAGATC CTGCAGGCGC AACTGGATTG CATCTGGCAT 
CAGCTTAATT CTCAAACAAA GTTGATAGTA GCAGATCTTA AGATTCTGCG CAGCTTAATG 
ATCTCCACCA TGTACCACGA TGCTGTGAGC GCATATGCCT TTATGAAACG CTACCGGAGC 
ACGGAGTACG CGTTGAGCAA CTCAGGATGG ACTTTACTGG ACGCCGCCGA ACAGATCTTT 
AAACTATCCA GGCAGCGTGT TTTCAATGGC CAGCAGGAGT TCGAACCGGA ACCCTGTCCC 
AAATGGCAAA CTCTTACGGA TTTGCTTACC AAGGAAATAC CCGGCGATAT GCGGCGGAGT 
AGGCGATCGG AACAACAACC GAAAGTGCTG ATCCTCTGCC AGGATGCACG GACGTGTCAT 
CAGTTGAAAC AGTATCTCAC GCAAGGTGGG CCACGTTTTC TACTCCAGCA GGCATTGCAA 
CATGAAGTGC CGGTGGGCAA ACTCAGTGAC AATTACGCCA AAGAGAGCCA AACTCGTTCA 
GCTCCGCCAA AGAATGTTTC ATCCAATAAG GAGCTGCGTA GGGAAGAGGT TTCTGGTTCT 
CAGCCACCTT TAGCTGGTAT GGATGAATTG GCCCAGCTTT TGAGCGAATC GGAAACAGAG 
GGTCAGCACT TCGAAGAGAG TTACATGCTC ACCATGACGC AGCCTGTGGA AGTCGGGCCA 
GCAGCGATAG ACATTAAACC CGATCCGGAT GTGTCTATTT TCGAGACAAT ACCGGAACTA 
GAACAATTTG ATGTCACTGC GGCCCTGGCC TCTGTGCCAC ATCAGCCATA CATTTGCTTG 
CAGACCTTCA AAACGGAGAG GGAGGGCTCC ATGGCACTGG AGCACATGCT GGAGCAACTG 
CAGCCGCACT ATGTTGTCAT GTACAACATG AATGTGACAG CCATTCGGCA ATTGGAGGTT 
TTCGAGGCCA GACGTCGCCT GCCGCCTGCA GATCGCATGA AGGTGTATTT CCTGATCCAT 
GCACGCACAG TGGAGGAGCA AGCCTATCTG ACCAGTTTGC GTCGAGAAAA GGCAGCCTTC 
GAGTTCATTA TCGACACTAA AAGTAAAATG GTGATTCCAA AGTACCAGGA TGGAAAGACC 
GATGAAGCGT TTTTGTTATT AAAGACTTAC GATGATGAGC CGACGGACGA GAATGCCAAG 
AGTCGCCAAG CGGGTGGGCA AGCTCCGCAG GCGACCAAAG AGACGCCAAA AGTGATTGTG 
GATATGCGTG AGTTTCGCTC AGATCTGCCC TGTTTGATTC ACAAGCGTGG TCTGGAAGTG 
CTGCCCTTGA CCATTACGAT TGGCGATTAT ATACTCACAC CGGATATTTG TGTGGAGCGA 
AAGTCCATTT CTGATTTGAT TGGGTCTCTG AATTCGGGCA GATTGTACAA CCAATGCGTG 
CAAATGCAGC GGCATTATGC AAAGCCCATC CTTTTGATTG AGTTCGATCA GAATAAGCCC 
TTTCACCTGC AGGGCAAATT TATGTTATCG CAGCAGACCA GCATGGCCAA TGCAGATATT 
GTGCAGAAAC TGCAACTTCT TACGTTACAT TTTCCCAAGC TGCGACTCAT TTGGTCACCC 
AGTCCGTATG CCACTGCCCA GCTATTCGAG GAGCTGAAGC TGGGTAAACC GGAACCGGAT 
CCTCAAACGG CGGCCGCCTT GGGCAGCGAC GAGCCAACGG CGGGTGAGCA ATTGCATTTC 
AACAGCGGCA TCTACGATTT CCTGCTACGT CTGCCCGGCG TTCATACGCG CAATATACAT 
GGCCTACTCC GGAAGGGCGG CAGCCTGCGG CAATTGTTGC TGCGCAGCCA GAAGGAACTG 
GAGGAGCTGC TGCAGTCGCA GGAGAGCGCC AAGCTGCTGT ACGATATCCT GCATGTGGCC 
CATTTGCCGG AGAAGGACGA GGTTACCGGC TCGACGGCGC TGCTGGCGGC TAGCAAGCAA 
TTTGGCGCCG GATCGCACAA TCGCTTCAGG ATGGCGGCAG CCGCATCGCG AAGGGGTCGC 
CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mei-9 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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