brn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	brn
Entrez Gene ID	31358
Full Name	brainiac
Synonyms	Brn,CG4934,Dmel\CG4934,EG:EG0007.6,brc,fs(1)107,fs(1)A107,l(1)6P6
General protein information	Preferred Names brainiac Names CG4934 gene product from transcript CG4934-RA CG4934-PA brn-PA gene B
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4A5-4A5\|1-5.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057553.5	NP_476901.1	brainiac

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	brn	NP_476901.1
Xenopus tropicalis (tropical clawed frog)	LOC100485796	XP_002941820.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002097	XP_320943.5
Xenopus tropicalis (tropical clawed frog)	b3galt2	XP_002941825.1
Caenorhabditis elegans (roundworm)	bre-5	NP_001255612.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Egghead and brainiac are essential for glycosphingolipid biosynthesis
Title: Egghead and brainiac are essential for glycosphingolipid biosynthesis in vivo.

encodes a beta 1,4-mannosyltransferase predicted to form the immediate precursor glycosphingolipid substrate for brainiac
Title: Drosophila egghead encodes a beta 1,4-mannosyltransferase predicted to form the immediate precursor glycosphingolipid substrate for brainiac.

Brainiac has a function as glycolipid-specific beta 1,3N-acetylglucosaminyltransferase.
Title: The Drosophila melanogaster brainiac protein is a glycolipid-specific beta 1,3N-acetylglucosaminyltransferase.

Role of brainiac as glycosyltransferase involved in glycosphingolipid synthesis
Title: The Drosophila gene brainiac encodes a glycosyltransferase putatively involved in glycosphingolipid synthesis.

The following brn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00751	NM_057553.5 Latest version!	Drosophila melanogaster brainiac (brn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

brn ( NM_057553.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476901.1 Drosophila melanogaster brainiac (brn), mRNA.

Price & Availability↑

CloneID	OFa00751
Clone ID Related Accession (Same CDS sequence)	NM_057553.5
Accession Version	NM_057553.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	brainiac
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057553.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAAGTA AACACCGCAA ACTGCTGCTG CGCTGCCTCC TGGTGCTGCC CCTCATCCTT 
CTGGTGGACT ATTGCGGCCT GCTGACCCAC CTGCACGAGC TGAACTTCGA GCGGCACTTT 
CACTACCCGC TGAACGATGA CACTGGATCT GGGTCCGCAT CCTCTGGACT GGACAAGTTC 
GCCTACCTGC GAGTGCCCTC GTTCACGGCA GAGGTGCCGG TTGACCAGCC TGCGCGGCTC 
ACGATGCTCA TAAAGAGCGC GGTGGGCAAT TCGCGACGTC GGGAGGCCAT TCGCCGGACT 
TGGGGCTACG AGGGTCGATT CTCAGACGTC CATCTGCGTC GCGTGTTCCT GCTGGGCACG 
GCCGAGGATA GTGAGAAAGA CGTGGCTTGG GAGTCCCGCG AGCATGGCGA CATTCTGCAG 
GCGGAGTTCA CGGACGCCTA CTTCAACAAC ACGCTGAAAA CCATGCTGGG TATGCGCTGG 
GCTAGCGATC AGTTCAATCG CAGCGAGTTC TATCTTTTCG TGGACGACGA CTACTATGTT 
TCGGCCAAGA ACGTGCTCAA GTTTCTGGGC AGGGGGCGTC AGAGCCACCA GCCGGAGTTG 
CTGTTCGCCG GGCACGTGTT TCAGACATCC CCGCTCCGCC ACAAGTTCAG CAAGTGGTAC 
GTTTCGTTGG AGGAATACCC CTTCGATCGG TGGCCACCGT ACGTGACCGC CGGCGCGTTC 
ATTCTGTCGC AGAAGGCACT GCGCCAGCTG TATGCCGCCA GCGTTCACCT GCCGCTCTTC 
CGGTTCGACG ACGTCTACCT GGGCATAGTG GCCTTGAAAG CGGGCATTTC CCTTCAGCAC 
TGCGACGACT TCCGCTTCCA TCGGCCGGCG TACAAAGGTC CGGACAGCTA TAGTTCGGTG 
ATAGCCTCGC ACGAATTCGG CGATCCCGAG GAGATGACGC GGGTATGGAA CGAGTGCCGA 
TCCGCCAATT ACGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476901.1
CDS	145..1122
Translation	MQSKHRKLLLRCLLVLPLILLVDYCGLLTHLHELNFERHFHYPLNDDTGSGSASSGLDKFAYLRVPSFTAEVPVDQPARLTMLIKSAVGNSRRREAIRRTWGYEGRFSDVHLRRVFLLGTAEDSEKDVAWESREHGDILQAEFTDAYFNNTLKTMLGMRWASDQFNRSEFYLFVDDDYYVSAKNVLKFLGRGRQSHQPELLFAGHVFQTSPLRHKFSKWYVSLEEYPFDRWPPYVTAGAFILSQKALRQLYAASVHLPLFRFDDVYLGIVALKAGISLQHCDDFRFHRPAYKGPDSYSSVIASHEFGDPEEMTRVWNECRSANYA

Target ORF information:

RefSeq Version	NM_057553.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster brainiac (brn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057553.5

ORF Insert Sequence:

ATGCAAAGTA AACACCGCAA ACTGCTGCTG CGCTGCCTCC TGGTGCTGCC CCTCATCCTT 
CTGGTGGACT ATTGCGGCCT GCTGACCCAC CTGCACGAGC TGAACTTCGA GCGGCACTTT 
CACTACCCGC TGAACGATGA CACTGGATCT GGGTCCGCAT CCTCTGGACT GGACAAGTTC 
GCCTACCTGC GAGTGCCCTC GTTCACGGCA GAGGTGCCGG TTGACCAGCC TGCGCGGCTC 
ACGATGCTCA TAAAGAGCGC GGTGGGCAAT TCGCGACGTC GGGAGGCCAT TCGCCGGACT 
TGGGGCTACG AGGGTCGATT CTCAGACGTC CATCTGCGTC GCGTGTTCCT GCTGGGCACG 
GCCGAGGATA GTGAGAAAGA CGTGGCTTGG GAGTCCCGCG AGCATGGCGA CATTCTGCAG 
GCGGAGTTCA CGGACGCCTA CTTCAACAAC ACGCTGAAAA CCATGCTGGG TATGCGCTGG 
GCTAGCGATC AGTTCAATCG CAGCGAGTTC TATCTTTTCG TGGACGACGA CTACTATGTT 
TCGGCCAAGA ACGTGCTCAA GTTTCTGGGC AGGGGGCGTC AGAGCCACCA GCCGGAGTTG 
CTGTTCGCCG GGCACGTGTT TCAGACATCC CCGCTCCGCC ACAAGTTCAG CAAGTGGTAC 
GTTTCGTTGG AGGAATACCC CTTCGATCGG TGGCCACCGT ACGTGACCGC CGGCGCGTTC 
ATTCTGTCGC AGAAGGCACT GCGCCAGCTG TATGCCGCCA GCGTTCACCT GCCGCTCTTC 
CGGTTCGACG ACGTCTACCT GGGCATAGTG GCCTTGAAAG CGGGCATTTC CCTTCAGCAC 
TGCGACGACT TCCGCTTCCA TCGGCCGGCG TACAAAGGTC CGGACAGCTA TAGTTCGGTG 
ATAGCCTCGC ACGAATTCGG CGATCCCGAG GAGATGACGC GGGTATGGAA CGAGTGCCGA 
TCCGCCAATT ACGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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brn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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