N cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	N
Entrez Gene ID	31293
Full Name	Notch
Synonyms	1.1,16-178,16-55,Ax,CG3936,CT13012,Chp,Co,Dmel\CG3936,EG:140G11.1,EG:163A10.2,NECD,NICD,RICN,anon-EST:Liang-1.12,clone 1.12,co,dNotch,fa,l(1)3Cb,l(1)Ax,l(1)N,n[fah],nd,notch,shd,spl,swb
General protein information	Preferred Names Notch Names CG3936 gene product from transcript CG3936-RA CG3936-PA CG3936-PB N-PA N-PB NICD abruptex chopped confluens dNotch facet notch notchoid split strawberry
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3C7-3C9\|1-3.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057511.4	NP_476859.2	notch, isoform A
NM_001258581.2	NP_001245510.1	notch, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NOTCH1	XP_002691666.2
Gallus gallus (chicken)	NOTCH1	XP_415420.4
Pan troglodytes (chimpanzee)	NOTCH1	XP_520371.2
	LOC709947	XP_002808138.1
Rattus norvegicus (Norway rat)	Notch1	NP_001099191.1
Drosophila melanogaster (fruit fly)	N	NP_001245510.1
Mus musculus (house mouse)	Notch1	NP_032740.3
Canis lupus familiaris (dog)	NOTCH1	XP_005625490.1
Homo sapiens (human)	NOTCH1	NP_060087.3
Danio rerio (zebrafish)	notch1b	NP_571377.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001015	XP_309208.5
Xenopus tropicalis (tropical clawed frog)	notch1	NP_001090757.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Grh, AbdA, and Exd function together at multiple motifs on the apoptotic enhancer. Exd-Grh-Notch work with different Hox genes through region specific enhancers to pattern respective segments of larval central nervous system.
Title: Combinatorial action of Grainyhead, Extradenticle and Notch in regulating Hox mediated apoptosis in Drosophila larval CNS.

our data indicate that the MLF/DnaJ-1-dependent increase in Lz level allows the repression of Notch expression and signaling to prevent aberrant blood cell development. Thus our findings establish a functional link between MLF and the co-chaperone DnaJ-1 to control RUNX transcription factor activity and Notch signaling during blood cell development in vivo.
Title: Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.

Notch signaling directly regulates ban expression at the transcriptional level to impact cell growth.
Title: The bantam microRNA acts through Numb to exert cell growth control and feedback regulation of Notch in tumor-forming stem cells in the Drosophila brain.

Nerfin-1 represses Notch activity in medulla neurons and prevents them from dedifferentiation.
Title: Prevention of medulla neuron dedifferentiation by Nerfin-1 requires inhibition of Notch activity.

To identify structural principles underlying polyQ tracts in disordered regulatory domains, study analyzed deep evolution of metazoan Notch polyQ tracts. Results suggest that intrinsically disordered interference arrays featuring carboxamide and polyQ enrichment may constitute coupled proteodynamic modulators of solenoids.
Title: Evolving Notch polyQ tracts reveal possible solenoid interference elements.

The following N gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00677	NM_001258581.2 Latest version!	Drosophila melanogaster notch, transcript variant B (N), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa00677	NM_057511.4 Latest version!	Drosophila melanogaster notch, transcript variant A (N), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

N ( NM_057511.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476859.2 Drosophila melanogaster notch, transcript variant A (N), mRNA.

Price & Availability↑

CloneID	OFa00677
Clone ID Related Accession (Same CDS sequence)	NM_057511.4 , NM_001258581.2
Accession Version	NM_057511.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	notch, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057511.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCGC AGCGCAGCCG ACGTCGCAGT CGCGCACCAA ACACTTGGAT TTGTTTTTGG 
ATTAACAAAA TGCACGCCGT TGCGTCGCTG CCGGCGTCGC TGCCTCTGCT GCTGTTGACG 
CTGGCGTTTG CGAATTTGCC AAACACCGTT CGCGGAACTG ATACCGCGTT GGTGGCCGCT 
TCCTGCACAA GTGTCGGTTG CCAGAATGGC GGCACATGCG TTACACAACT CAATGGCAAA 
ACCTACTGCG CATGCGATTC GCACTATGTC GGCGACTACT GTGAACACCG CAATCCGTGC 
AATTCGATGC GTTGCCAAAA TGGTGGCACC TGTCAGGTGA CCTTTCGCAA CGGCCGTCCG 
GGCATCTCGT GCAAGTGTCC TTTGGGCTTC GACGAGTCCC TGTGCGAAAT TGCGGTACCG 
AATGCCTGCG ATCATGTGAC CTGCCTCAAT GGAGGCACCT GTCAGCTAAA GACACTGGAG 
GAGTACACGT GTGCGTGTGC CAATGGCTAT ACAGGTGAAC GATGCGAGAC GAAGAATCTG 
TGCGCAAGTT CTCCCTGCCG GAATGGAGCC ACCTGCACCG CTTTGGCCGG AAGCAGCAGC 
TTCACCTGCT CCTGTCCGCC GGGCTTCACC GGTGACACCT GTTCCTATGA CATCGAGGAG 
TGCCAGTCGA ATCCGTGCAA ATACGGCGGC ACATGTGTCA ACACCCACGG ATCTTACCAG 
TGCATGTGTC CCACGGGCTA CACGGGAAAG GATTGCGACA CCAAGTACAA GCCCTGCTCG 
CCATCGCCGT GCCAGAATGG CGGAATTTGC CGGTCGAACG GTCTGTCCTA CGAGTGCAAG 
TGCCCCAAAG GTTTCGAGGG CAAGAACTGC GAACAGAACT ACGACGATTG CCTGGGACAT 
CTTTGCCAGA ACGGAGGAAC CTGCATCGAT GGCATCTCGG ACTACACCTG CCGCTGTCCA 
CCGAACTTCA CGGGCAGATT CTGCCAGGAC GATGTGGACG AGTGCGCCCA ACGGGATCAT 
CCGGTGTGCC AGAACGGAGC CACCTGTACA AACACTCACG GATCGTACAG CTGCATCTGC 
GTGAATGGCT GGGCGGGCTT GGACTGCAGC AACAACACGG ATGACTGCAA ACAGGCGGCC 
TGTTTCTACG GAGCCACGTG CATCGATGGC GTTGGCAGCT TCTATTGCCA GTGTACGAAG 
GGCAAGACGG GTCTGTTGTG CCACCTGGAC GATGCCTGTA CCTCGAATCC CTGCCATGCG 
GACGCCATTT GTGACACGAG TCCGATAAAC GGCTCCTATG CCTGTTCCTG TGCCACCGGC 
TACAAGGGCG TGGATTGTTC AGAGGACATA GATGAATGCG ATCAGGGATC ACCGTGCGAA 
CACAATGGCA TATGCGTGAA CACACCGGGC AGCTACAGAT GCAATTGCTC CCAGGGCTTT 
ACGGGTCCGC GCTGCGAGAC GAACATCAAT GAGTGCGAAT CGCATCCATG CCAGAACGAG 
GGATCTTGCC TGGATGATCC GGGAACGTTC CGATGCGTCT GTATGCCAGG ATTCACGGGC 
ACACAGTGCG AAATCGACAT TGACGAATGC CAATCGAATC CCTGCTTGAA CGATGGAACT 
TGCCACGACA AGATCAATGG CTTTAAATGC AGTTGTGCCC TGGGCTTTAC GGGCGCACGA 
TGTCAAATCA ATATAGACGA CTGTCAGTCG CAACCATGCC GGAATCGTGG CATCTGTCAC 
GACTCTATAG CGGGCTACAG CTGCGAATGT CCGCCGGGAT ATACGGGCAC CAGTTGCGAG 
ATCAATATCA ACGATTGCGA TTCGAATCCC TGCCATCGGG GCAAGTGCAT CGACGATGTG 
AACAGTTTCA AGTGCCTCTG TGATCCCGGC TACACGGGCT ACATATGCCA GAAGCAGATC 
AACGAATGCG AATCGAATCC GTGCCAGTTC GATGGCCATT GCCAGGATCG TGTGGGCAGC 
TACTATTGTC AGTGCCAGGC GGGAACCAGT GGCAAGAATT GCGAGGTCAA TGTGAACGAA 
TGCCACAGTA ATCCCTGCAA CAATGGTGCC ACCTGCATCG ATGGCATCAA TTCGTACAAG 
TGCCAGTGTG TGCCCGGTTT TACGGGTCAG CACTGCGAGA AGAACGTCGA CGAGTGCATT 
AGCAGTCCGT GTGCCAACAA TGGTGTCTGC ATAGATCAGG TGAATGGCTA CAAGTGCGAG 
TGTCCGCGTG GATTCTACGA TGCCCATTGC CTGAGCGATG TGGACGAGTG CGCATCGAAT 
CCGTGCGTGA ACGAGGGACG TTGCGAGGAT GGAATCAATG AGTTCATCTG CCACTGTCCG 
CCAGGATACA CGGGCAAGCG TTGTGAGCTG GACATAGACG AGTGCAGCAG CAATCCCTGC 
CAGCATGGTG GCACCTGTTA TGATAAGCTC AACGCCTTCA GTTGCCAATG CATGCCGGGC 
TATACGGGCC AAAAGTGCGA AACGAATATT GACGACTGTG TGACCAATCC GTGCGGAAAT 
GGAGGCACCT GCATTGACAA GGTCAACGGC TACAAGTGCG TCTGCAAAGT GCCCTTCACG 
GGCAGGGATT GTGAAAGCAA AATGGATCCC TGCGCCAGCA ATCGTTGCAA AAACGAGGCG 
AAATGCACGC CAAGCTCGAA TTTCCTGGAC TTCTCCTGCA CCTGCAAACT GGGCTATACG 
GGTCGCTATT GCGATGAGGA CATAGACGAA TGCTCACTGA GTTCACCCTG TCGGAATGGA 
GCTAGCTGTT TGAATGTTCC CGGATCGTAT AGGTGCCTCT GCACCAAGGG CTATGAGGGC 
AGAGATTGCG CCATCAATAC GGACGATTGT GCCTCGTTTC CGTGCCAGAA CGGTGGAACC 
TGTCTGGATG GGATCGGTGA CTATAGCTGC CTGTGCGTGG ATGGCTTCGA TGGCAAGCAC 
TGCGAGACGG ACATCAATGA GTGCTTGAGT CAGCCGTGCC AGAATGGAGC CACGTGTAGT 
CAGTATGTGA ATAGCTATAC CTGCACCTGT CCACTCGGAT TCTCCGGCAT CAATTGTCAG 
ACGAACGATG AGGATTGCAC GGAGAGCTCG TGCTTAAACG GTGGCAGTTG CATCGATGGC 
ATCAATGGCT ACAACTGTAG CTGTTTGGCT GGATATTCCG GTGCCAATTG TCAGTACAAG 
TTGAATAAGT GCGATTCGAA TCCCTGTTTG AACGGAGCCA CCTGCCACGA GCAAAACAAC 
GAGTACACAT GCCATTGTCC CAGTGGCTTT ACGGGCAAAC AGTGTTCCGA ATATGTGGAT 
TGGTGTGGTC AATCGCCGTG CGAGAATGGA GCGACCTGTA GCCAGATGAA GCATCAGTTT 
AGCTGCAAAT GCTCGGCAGG ATGGACGGGT AAGCTGTGCG ATGTTCAGAC GATTTCGTGT 
CAGGATGCAG CAGATCGGAA GGGTCTGAGC CTGCGGCAGT TGTGCAACAA TGGCACGTGC 
AAGGATTATG GAAATAGTCA TGTGTGCTAC TGCTCCCAGG GATACGCGGG TAGCTATTGC 
CAAAAGGAGA TCGACGAGTG CCAATCGCAA CCGTGCCAGA ATGGTGGAAC GTGCCGAGAT 
CTCATCGGAG CCTACGAATG CCAGTGTCGC CAGGGATTTC AGGGTCAGAA TTGCGAACTG 
AACATCGATG ACTGTGCACC GAATCCCTGC CAGAATGGCG GCACTTGCCA CGACCGTGTG 
ATGAACTTTA GCTGCAGCTG TCCACCGGGA ACGATGGGCA TCATATGCGA GATCAACAAG 
GATGATTGCA AACCGGGAGC ATGCCACAAC AACGGCAGCT GCATCGATCG CGTTGGAGGC 
TTCGAGTGCG TCTGTCAGCC CGGTTTTGTG GGTGCCCGCT GCGAGGGCGA CATCAACGAG 
TGCCTAAGCA ATCCCTGTTC GAATGCCGGC ACACTGGACT GCGTCCAGTT GGTCAATAAC 
TATCACTGCA ACTGTCGACC TGGACATATG GGTCGGCACT GTGAGCACAA GGTGGACTTT 
TGTGCCCAGA GTCCGTGCCA GAATGGTGGC AATTGTAATA TCCGACAGAG TGGCCACCAC 
TGCATCTGTA ATAATGGATT CTACGGTAAG AACTGTGAGC TGAGTGGTCA GGATTGTGAT 
TCGAATCCCT GCCGTGTGGG TAACTGTGTT GTCGCGGACG AGGGATTCGG CTATAGATGC 
GAGTGTCCAA GGGGCACACT GGGTGAGCAC TGCGAGATTG ATACGCTAGA CGAATGTTCG 
CCGAATCCCT GTGCCCAGGG AGCTGCCTGC GAGGATTTGC TCGGTGATTA CGAATGCCTC 
TGTCCGAGCA AATGGAAAGG CAAACGCTGC GATATCTATG ATGCCAACTA TCCGGGATGG 
AATGGCGGTT CGGGATCCGG AAATGATCGC TATGCCGCTG ATTTGGAGCA ACAACGTGCC 
ATGTGCGATA AGCGAGGTTG CACCGAAAAG CAGGGCAACG GCATTTGCGA TTCCGATTGC 
AATACGTATG CCTGCAACTT TGATGGAAAC GATTGCTCAC TGGGCATCAA TCCGTGGGCC 
AATTGCACGG CCAACGAGTG CTGGAACAAA TTCAAGAACG GCAAATGCAA CGAGGAGTGC 
AACAATGCTG CCTGCCACTA CGATGGTCAC GATTGCGAAC GGAAATTGAA GAGTTGCGAT 
AGCCTTTTCG ATGCCTACTG CCAGAAGCAT TACGGCGATG GTTTCTGCGA CTATGGATGC 
AATAATGCGG AATGCAGTTG GGACGGACTC GACTGTGAGA ACAAGACCCA GTCACCGGTT 
TTGGCCGAGG GTGCCATGTC CGTGGTGATG CTAATGAATG TGGAGGCATT CCGCGAGATT 
CAGGCTCAGT TCCTTAGGAA TATGAGCCAC ATGCTGCGGA CAACGGTGCG GTTGAAGAAG 
GACGCTCTTG GTCATGACAT CATTATCAAT TGGAAGGACA ATGTGCGCGT GCCGGAGATC 
GAGGATACGG ACTTTGCGAG AAAAAACAAG ATTCTGTACA CCCAACAGGT CCATCAGACG 
GGCATTCAAA TCTATTTGGA GATTGACAAT CGCAAATGCA CCGAATGCTT TACGCACGCC 
GTTGAGGCAG CCGAATTTCT GGCTGCCACG GCGGCCAAAC ATCAGCTGCG GAACGATTTT 
CAGATACACA GTGTTAGGGG CATCAAGAAT CCCGGCGATG AGGACAATGG TGAGCCGCCG 
GCGAATGTGA AATACGTAAT TACTGGCATT ATACTGGTCA TCATTGCATT GGCCTTCTTT 
GGCATGGTCT TGAGTACGCA AAGAAAGCGG GCACATGGCG TCACCTGGTT CCCGGAGGGA 
TTCCGTGCAC CGGCGGCGGT TATGTCGCGA CGTCGACGTG ATCCACACGG CCAAGAGATG 
CGGAATCTCA ACAAGCAGGT GGCCATGCAA TCGCAGGGTG TTGGCCAGCC AGGTGCTCAT 
TGGTCCGACG ATGAATCGGA TATGCCGTTG CCCAAGCGAC AGCGCAGTGA TCCCGTTTCC 
GGTGTCGGTC TGGGCAACAA TGGTGGCTAT GCCAGTGATC ATACAATGGT CAGCGAATAT 
GAGGAGGCTG ATCAGCGGGT CTGGTCGCAG GCCCATCTGG ATGTGGTCGA TGTGCGGGCC 
ATAATGACAC CGCCGGCGCA TCAGGATGGT GGCAAGCACG ATGTGGATGC ACGTGGACCT 
TGCGGACTGA CTCCGCTAAT GATTGCTGCC GTACGAGGCG GTGGCCTGGA CACTGGCGAG 
GATATCGAGA ACAATGAGGA CAGTACTGCC CAGGTGATAT CGGATCTATT GGCACAGGGA 
GCCGAGTTAA ATGCCACAAT GGATAAAACG GGCGAGACAT CATTGCATTT GGCAGCACGT 
TTTGCTCGAG CAGATGCCGC CAAGAGATTA TTGGACGCCG GTGCGGATGC CAATTGTCAG 
GATAACACGG GCAGGACACC GCTGCACGCT GCTGTGGCCG CCGATGCCAT GGGTGTATTT 
CAGATATTGC TTAGGAATAG GGCTACCAAT CTAAATGCTA GAATGCATGA CGGCACAACG 
CCACTAATAC TGGCCGCTCG TCTAGCCATC GAGGGCATGG TGGAGGATTT GATCACAGCC 
GATGCGGATA TTAATGCAGC GGATAATTCC GGCAAGACCG CTCTCCATTG GGCAGCGGCG 
GTTAACAATA CCGAAGCGGT GAACATTCTG CTAATGCATC ATGCCAATCG CGATGCCCAG 
GACGATAAGG ATGAGACACC GCTGTTTCTG GCCGCACGCG AGGGTAGCTA TGAGGCGTGC 
AAGGCGCTGT TGGATAATTT TGCCAATCGC GAGATTACCG ATCACATGGA TCGATTGCCA 
CGCGATGTGG CCAGCGAGCG ACTGCATCAC GATATTGTCA GGCTGCTGGA CGAGCATGTG 
CCGCGATCTC CGCAAATGCT GAGCATGACA CCGCAGGCAA TGATCGGATC CCCGCCGCCG 
GGTCAACAGC AGCCGCAGTT GATCACACAG CCGACGGTCA TTTCCGCCGG AAATGGTGGC 
AACAATGGCA ATGGCAATGC CAGCGGAAAG CAGAGCAACC AAACGGCCAA ACAGAAGGCG 
GCCAAGAAGG CCAAACTAAT CGAGGGCAGT CCGGACAATG GGCTGGATGC CACGGGCAGT 
TTGCGACGCA AGGCAAGTTC AAAAAAGACA AGTGCGGCCT CAAAAAAGGC CGCCAACTTG 
AATGGCCTAA ATCCGGGCCA GCTGACGGGG GGAGTGTCGG GCGTGCCGGG TGTACCGCCA 
ACGAATTCAG CGGCACAAGC AGCTGCAGCG GCAGCGGCAG CCGTGGCGGC CATGTCCCAC 
GAACTGGAGG GTTCGCCGGT GGGCGTGGGC ATGGGCGGGA ATCTGCCCAG TCCGTACGAC 
ACCAGTTCGA TGTACTCGAA TGCGATGGCC GCACCGCTGG CGAACGGCAA TCCGAACACG 
GGCGCCAAGC AGCCGCCGAG CTATGAGGAT TGCATCAAGA ATGCGCAATC GATGCAATCG 
CTGCAGGGCA ATGGTCTGGA TATGATCAAG CTGGATAATT ATGCCTACTC GATGGGCTCG 
CCATTTCAGC AGGAGCTGCT CAATGGCCAA GGACTCGGGA TGAATGGTAA TGGCCAGCGA 
AATGGAGTCG GTCCCGGCGT TCTGCCCGGC GGCCTATGCG GAATGGGCGG TCTGAGTGGA 
GCCGGCAATG GAAATAGCCA CGAACAGGGA CTGAGTCCGC CGTACTCGAA TCAGTCGCCG 
CCGCATTCGG TGCAGAGCAG CCTGGCGCTA TCGCCCCACG CCTACTTGGG CTCGCCATCG 
CCGGCCAAGT CGCGACCCAG TCTACCCACC TCGCCCACCC ACATTCAGGC GATGAGGCAT 
GCCACACAGC AGAAGCAATT CGGTGGCAGT AATCTGAATA GTTTGCTGGG CGGTGCCAAT 
GGCGGGGGCG TGGTCGGCGG AGGAGGAGGT GGTGGTGGTG GTGTTGGCCA GGGGCCACAA 
AACTCACCCG TAAGCTTGGG AATCATCTCG CCGACGGGCA GCGATATGGG CATCATGCTC 
GCCCCGCCCC AATCCTCGAA GAATAGTGCA ATAATGCAAA CGATATCACC CCAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAA CATCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAGC AACAGCAGCA GCAACTCGGA GGCCTGGAGT TCGGTTCAGC GGGCTTGGAC 
CTGAATGGAT TTTGTGGATC TCCGGACTCA TTTCACTCGG GTCAAATGAA TCCGCCCTCG 
ATACAAAGTT CAATGTCCGG CTCATCGCCG TCGACCAATA TGTTGTCGCC GTCGTCGCAG 
CACAATCAGC AGGCCTTCTA CCAGTACCTA ACGCCTTCGA GCCAACATTC CGGCGGCCAT 
ACGCCGCAGC ATTTGGTCCA AACGTTGGAT AGCTATCCGA CGCCCTCGCC GGAGTCACCT 
GGCCACTGGT CCTCCTCGTC GCCGCGATCG AATTCCGATT GGAGCGAGGG CGTTCAGTCG 
CCGGCGGCAA ATAATCTTTA CATATCCGGT GGCCATCAGG CTAACAAGGG TTCCGAGGCC 
ATCTACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476859.2
CDS	799..8910
Translation	MQSQRSRRRSRAPNTWICFWINKMHAVASLPASLPLLLLTLAFANLPNTVRGTDTALVAASCTSVGCQNGGTCVTQLNGKTYCACDSHYVGDYCEHRNPCNSMRCQNGGTCQVTFRNGRPGISCKCPLGFDESLCEIAVPNACDHVTCLNGGTCQLKTLEEYTCACANGYTGERCETKNLCASSPCRNGATCTALAGSSSFTCSCPPGFTGDTCSYDIEECQSNPCKYGGTCVNTHGSYQCMCPTGYTGKDCDTKYKPCSPSPCQNGGICRSNGLSYECKCPKGFEGKNCEQNYDDCLGHLCQNGGTCIDGISDYTCRCPPNFTGRFCQDDVDECAQRDHPVCQNGATCTNTHGSYSCICVNGWAGLDCSNNTDDCKQAACFYGATCIDGVGSFYCQCTKGKTGLLCHLDDACTSNPCHADAICDTSPINGSYACSCATGYKGVDCSEDIDECDQGSPCEHNGICVNTPGSYRCNCSQGFTGPRCETNINECESHPCQNEGSCLDDPGTFRCVCMPGFTGTQCEIDIDECQSNPCLNDGTCHDKINGFKCSCALGFTGARCQINIDDCQSQPCRNRGICHDSIAGYSCECPPGYTGTSCEININDCDSNPCHRGKCIDDVNSFKCLCDPGYTGYICQKQINECESNPCQFDGHCQDRVGSYYCQCQAGTSGKNCEVNVNECHSNPCNNGATCIDGINSYKCQCVPGFTGQHCEKNVDECISSPCANNGVCIDQVNGYKCECPRGFYDAHCLSDVDECASNPCVNEGRCEDGINEFICHCPPGYTGKRCELDIDECSSNPCQHGGTCYDKLNAFSCQCMPGYTGQKCETNIDDCVTNPCGNGGTCIDKVNGYKCVCKVPFTGRDCESKMDPCASNRCKNEAKCTPSSNFLDFSCTCKLGYTGRYCDEDIDECSLSSPCRNGASCLNVPGSYRCLCTKGYEGRDCAINTDDCASFPCQNGGTCLDGIGDYSCLCVDGFDGKHCETDINECLSQPCQNGATCSQYVNSYTCTCPLGFSGINCQTNDEDCTESSCLNGGSCIDGINGYNCSCLAGYSGANCQYKLNKCDSNPCLNGATCHEQNNEYTCHCPSGFTGKQCSEYVDWCGQSPCENGATCSQMKHQFSCKCSAGWTGKLCDVQTISCQDAADRKGLSLRQLCNNGTCKDYGNSHVCYCSQGYAGSYCQKEIDECQSQPCQNGGTCRDLIGAYECQCRQGFQGQNCELNIDDCAPNPCQNGGTCHDRVMNFSCSCPPGTMGIICEINKDDCKPGACHNNGSCIDRVGGFECVCQPGFVGARCEGDINECLSNPCSNAGTLDCVQLVNNYHCNCRPGHMGRHCEHKVDFCAQSPCQNGGNCNIRQSGHHCICNNGFYGKNCELSGQDCDSNPCRVGNCVVADEGFGYRCECPRGTLGEHCEIDTLDECSPNPCAQGAACEDLLGDYECLCPSKWKGKRCDIYDANYPGWNGGSGSGNDRYAADLEQQRAMCDKRGCTEKQGNGICDSDCNTYACNFDGNDCSLGINPWANCTANECWNKFKNGKCNEECNNAACHYDGHDCERKLKSCDSLFDAYCQKHYGDGFCDYGCNNAECSWDGLDCENKTQSPVLAEGAMSVVMLMNVEAFREIQAQFLRNMSHMLRTTVRLKKDALGHDIIINWKDNVRVPEIEDTDFARKNKILYTQQVHQTGIQIYLEIDNRKCTECFTHAVEAAEFLAATAAKHQLRNDFQIHSVRGIKNPGDEDNGEPPANVKYVITGIILVIIALAFFGMVLSTQRKRAHGVTWFPEGFRAPAAVMSRRRRDPHGQEMRNLNKQVAMQSQGVGQPGAHWSDDESDMPLPKRQRSDPVSGVGLGNNGGYASDHTMVSEYEEADQRVWSQAHLDVVDVRAIMTPPAHQDGGKHDVDARGPCGLTPLMIAAVRGGGLDTGEDIENNEDSTAQVISDLLAQGAELNATMDKTGETSLHLAARFARADAAKRLLDAGADANCQDNTGRTPLHAAVAADAMGVFQILLRNRATNLNARMHDGTTPLILAARLAIEGMVEDLITADADINAADNSGKTALHWAAAVNNTEAVNILLMHHANRDAQDDKDETPLFLAAREGSYEACKALLDNFANREITDHMDRLPRDVASERLHHDIVRLLDEHVPRSPQMLSMTPQAMIGSPPPGQQQPQLITQPTVISAGNGGNNGNGNASGKQSNQTAKQKAAKKAKLIEGSPDNGLDATGSLRRKASSKKTSAASKKAANLNGLNPGQLTGGVSGVPGVPPTNSAAQAAAAAAAAVAAMSHELEGSPVGVGMGGNLPSPYDTSSMYSNAMAAPLANGNPNTGAKQPPSYEDCIKNAQSMQSLQGNGLDMIKLDNYAYSMGSPFQQELLNGQGLGMNGNGQRNGVGPGVLPGGLCGMGGLSGAGNGNSHEQGLSPPYSNQSPPHSVQSSLALSPHAYLGSPSPAKSRPSLPTSPTHIQAMRHATQQKQFGGSNLNSLLGGANGGGVVGGGGGGGGGVGQGPQNSPVSLGIISPTGSDMGIMLAPPQSSKNSAIMQTISPQQQQQQQQQQQQQHQQQQQQQQQQQQQQQQQLGGLEFGSAGLDLNGFCGSPDSFHSGQMNPPSIQSSMSGSSPSTNMLSPSSQHNQQAFYQYLTPSSQHSGGHTPQHLVQTLDSYPTPSPESPGHWSSSSPRSNSDWSEGVQSPAANNLYISGGHQANKGSEAIYI

Target ORF information:

RefSeq Version	NM_057511.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster notch, transcript variant A (N), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057511.4

ORF Insert Sequence:

ATGCAATCGC AGCGCAGCCG ACGTCGCAGT CGCGCACCAA ACACTTGGAT TTGTTTTTGG 
ATTAACAAAA TGCACGCCGT TGCGTCGCTG CCGGCGTCGC TGCCTCTGCT GCTGTTGACG 
CTGGCGTTTG CGAATTTGCC AAACACCGTT CGCGGAACTG ATACCGCGTT GGTGGCCGCT 
TCCTGCACAA GTGTCGGTTG CCAGAATGGC GGCACATGCG TTACACAACT CAATGGCAAA 
ACCTACTGCG CATGCGATTC GCACTATGTC GGCGACTACT GTGAACACCG CAATCCGTGC 
AATTCGATGC GTTGCCAAAA TGGTGGCACC TGTCAGGTGA CCTTTCGCAA CGGCCGTCCG 
GGCATCTCGT GCAAGTGTCC TTTGGGCTTC GACGAGTCCC TGTGCGAAAT TGCGGTACCG 
AATGCCTGCG ATCATGTGAC CTGCCTCAAT GGAGGCACCT GTCAGCTAAA GACACTGGAG 
GAGTACACGT GTGCGTGTGC CAATGGCTAT ACAGGTGAAC GATGCGAGAC GAAGAATCTG 
TGCGCAAGTT CTCCCTGCCG GAATGGAGCC ACCTGCACCG CTTTGGCCGG AAGCAGCAGC 
TTCACCTGCT CCTGTCCGCC GGGCTTCACC GGTGACACCT GTTCCTATGA CATCGAGGAG 
TGCCAGTCGA ATCCGTGCAA ATACGGCGGC ACATGTGTCA ACACCCACGG ATCTTACCAG 
TGCATGTGTC CCACGGGCTA CACGGGAAAG GATTGCGACA CCAAGTACAA GCCCTGCTCG 
CCATCGCCGT GCCAGAATGG CGGAATTTGC CGGTCGAACG GTCTGTCCTA CGAGTGCAAG 
TGCCCCAAAG GTTTCGAGGG CAAGAACTGC GAACAGAACT ACGACGATTG CCTGGGACAT 
CTTTGCCAGA ACGGAGGAAC CTGCATCGAT GGCATCTCGG ACTACACCTG CCGCTGTCCA 
CCGAACTTCA CGGGCAGATT CTGCCAGGAC GATGTGGACG AGTGCGCCCA ACGGGATCAT 
CCGGTGTGCC AGAACGGAGC CACCTGTACA AACACTCACG GATCGTACAG CTGCATCTGC 
GTGAATGGCT GGGCGGGCTT GGACTGCAGC AACAACACGG ATGACTGCAA ACAGGCGGCC 
TGTTTCTACG GAGCCACGTG CATCGATGGC GTTGGCAGCT TCTATTGCCA GTGTACGAAG 
GGCAAGACGG GTCTGTTGTG CCACCTGGAC GATGCCTGTA CCTCGAATCC CTGCCATGCG 
GACGCCATTT GTGACACGAG TCCGATAAAC GGCTCCTATG CCTGTTCCTG TGCCACCGGC 
TACAAGGGCG TGGATTGTTC AGAGGACATA GATGAATGCG ATCAGGGATC ACCGTGCGAA 
CACAATGGCA TATGCGTGAA CACACCGGGC AGCTACAGAT GCAATTGCTC CCAGGGCTTT 
ACGGGTCCGC GCTGCGAGAC GAACATCAAT GAGTGCGAAT CGCATCCATG CCAGAACGAG 
GGATCTTGCC TGGATGATCC GGGAACGTTC CGATGCGTCT GTATGCCAGG ATTCACGGGC 
ACACAGTGCG AAATCGACAT TGACGAATGC CAATCGAATC CCTGCTTGAA CGATGGAACT 
TGCCACGACA AGATCAATGG CTTTAAATGC AGTTGTGCCC TGGGCTTTAC GGGCGCACGA 
TGTCAAATCA ATATAGACGA CTGTCAGTCG CAACCATGCC GGAATCGTGG CATCTGTCAC 
GACTCTATAG CGGGCTACAG CTGCGAATGT CCGCCGGGAT ATACGGGCAC CAGTTGCGAG 
ATCAATATCA ACGATTGCGA TTCGAATCCC TGCCATCGGG GCAAGTGCAT CGACGATGTG 
AACAGTTTCA AGTGCCTCTG TGATCCCGGC TACACGGGCT ACATATGCCA GAAGCAGATC 
AACGAATGCG AATCGAATCC GTGCCAGTTC GATGGCCATT GCCAGGATCG TGTGGGCAGC 
TACTATTGTC AGTGCCAGGC GGGAACCAGT GGCAAGAATT GCGAGGTCAA TGTGAACGAA 
TGCCACAGTA ATCCCTGCAA CAATGGTGCC ACCTGCATCG ATGGCATCAA TTCGTACAAG 
TGCCAGTGTG TGCCCGGTTT TACGGGTCAG CACTGCGAGA AGAACGTCGA CGAGTGCATT 
AGCAGTCCGT GTGCCAACAA TGGTGTCTGC ATAGATCAGG TGAATGGCTA CAAGTGCGAG 
TGTCCGCGTG GATTCTACGA TGCCCATTGC CTGAGCGATG TGGACGAGTG CGCATCGAAT 
CCGTGCGTGA ACGAGGGACG TTGCGAGGAT GGAATCAATG AGTTCATCTG CCACTGTCCG 
CCAGGATACA CGGGCAAGCG TTGTGAGCTG GACATAGACG AGTGCAGCAG CAATCCCTGC 
CAGCATGGTG GCACCTGTTA TGATAAGCTC AACGCCTTCA GTTGCCAATG CATGCCGGGC 
TATACGGGCC AAAAGTGCGA AACGAATATT GACGACTGTG TGACCAATCC GTGCGGAAAT 
GGAGGCACCT GCATTGACAA GGTCAACGGC TACAAGTGCG TCTGCAAAGT GCCCTTCACG 
GGCAGGGATT GTGAAAGCAA AATGGATCCC TGCGCCAGCA ATCGTTGCAA AAACGAGGCG 
AAATGCACGC CAAGCTCGAA TTTCCTGGAC TTCTCCTGCA CCTGCAAACT GGGCTATACG 
GGTCGCTATT GCGATGAGGA CATAGACGAA TGCTCACTGA GTTCACCCTG TCGGAATGGA 
GCTAGCTGTT TGAATGTTCC CGGATCGTAT AGGTGCCTCT GCACCAAGGG CTATGAGGGC 
AGAGATTGCG CCATCAATAC GGACGATTGT GCCTCGTTTC CGTGCCAGAA CGGTGGAACC 
TGTCTGGATG GGATCGGTGA CTATAGCTGC CTGTGCGTGG ATGGCTTCGA TGGCAAGCAC 
TGCGAGACGG ACATCAATGA GTGCTTGAGT CAGCCGTGCC AGAATGGAGC CACGTGTAGT 
CAGTATGTGA ATAGCTATAC CTGCACCTGT CCACTCGGAT TCTCCGGCAT CAATTGTCAG 
ACGAACGATG AGGATTGCAC GGAGAGCTCG TGCTTAAACG GTGGCAGTTG CATCGATGGC 
ATCAATGGCT ACAACTGTAG CTGTTTGGCT GGATATTCCG GTGCCAATTG TCAGTACAAG 
TTGAATAAGT GCGATTCGAA TCCCTGTTTG AACGGAGCCA CCTGCCACGA GCAAAACAAC 
GAGTACACAT GCCATTGTCC CAGTGGCTTT ACGGGCAAAC AGTGTTCCGA ATATGTGGAT 
TGGTGTGGTC AATCGCCGTG CGAGAATGGA GCGACCTGTA GCCAGATGAA GCATCAGTTT 
AGCTGCAAAT GCTCGGCAGG ATGGACGGGT AAGCTGTGCG ATGTTCAGAC GATTTCGTGT 
CAGGATGCAG CAGATCGGAA GGGTCTGAGC CTGCGGCAGT TGTGCAACAA TGGCACGTGC 
AAGGATTATG GAAATAGTCA TGTGTGCTAC TGCTCCCAGG GATACGCGGG TAGCTATTGC 
CAAAAGGAGA TCGACGAGTG CCAATCGCAA CCGTGCCAGA ATGGTGGAAC GTGCCGAGAT 
CTCATCGGAG CCTACGAATG CCAGTGTCGC CAGGGATTTC AGGGTCAGAA TTGCGAACTG 
AACATCGATG ACTGTGCACC GAATCCCTGC CAGAATGGCG GCACTTGCCA CGACCGTGTG 
ATGAACTTTA GCTGCAGCTG TCCACCGGGA ACGATGGGCA TCATATGCGA GATCAACAAG 
GATGATTGCA AACCGGGAGC ATGCCACAAC AACGGCAGCT GCATCGATCG CGTTGGAGGC 
TTCGAGTGCG TCTGTCAGCC CGGTTTTGTG GGTGCCCGCT GCGAGGGCGA CATCAACGAG 
TGCCTAAGCA ATCCCTGTTC GAATGCCGGC ACACTGGACT GCGTCCAGTT GGTCAATAAC 
TATCACTGCA ACTGTCGACC TGGACATATG GGTCGGCACT GTGAGCACAA GGTGGACTTT 
TGTGCCCAGA GTCCGTGCCA GAATGGTGGC AATTGTAATA TCCGACAGAG TGGCCACCAC 
TGCATCTGTA ATAATGGATT CTACGGTAAG AACTGTGAGC TGAGTGGTCA GGATTGTGAT 
TCGAATCCCT GCCGTGTGGG TAACTGTGTT GTCGCGGACG AGGGATTCGG CTATAGATGC 
GAGTGTCCAA GGGGCACACT GGGTGAGCAC TGCGAGATTG ATACGCTAGA CGAATGTTCG 
CCGAATCCCT GTGCCCAGGG AGCTGCCTGC GAGGATTTGC TCGGTGATTA CGAATGCCTC 
TGTCCGAGCA AATGGAAAGG CAAACGCTGC GATATCTATG ATGCCAACTA TCCGGGATGG 
AATGGCGGTT CGGGATCCGG AAATGATCGC TATGCCGCTG ATTTGGAGCA ACAACGTGCC 
ATGTGCGATA AGCGAGGTTG CACCGAAAAG CAGGGCAACG GCATTTGCGA TTCCGATTGC 
AATACGTATG CCTGCAACTT TGATGGAAAC GATTGCTCAC TGGGCATCAA TCCGTGGGCC 
AATTGCACGG CCAACGAGTG CTGGAACAAA TTCAAGAACG GCAAATGCAA CGAGGAGTGC 
AACAATGCTG CCTGCCACTA CGATGGTCAC GATTGCGAAC GGAAATTGAA GAGTTGCGAT 
AGCCTTTTCG ATGCCTACTG CCAGAAGCAT TACGGCGATG GTTTCTGCGA CTATGGATGC 
AATAATGCGG AATGCAGTTG GGACGGACTC GACTGTGAGA ACAAGACCCA GTCACCGGTT 
TTGGCCGAGG GTGCCATGTC CGTGGTGATG CTAATGAATG TGGAGGCATT CCGCGAGATT 
CAGGCTCAGT TCCTTAGGAA TATGAGCCAC ATGCTGCGGA CAACGGTGCG GTTGAAGAAG 
GACGCTCTTG GTCATGACAT CATTATCAAT TGGAAGGACA ATGTGCGCGT GCCGGAGATC 
GAGGATACGG ACTTTGCGAG AAAAAACAAG ATTCTGTACA CCCAACAGGT CCATCAGACG 
GGCATTCAAA TCTATTTGGA GATTGACAAT CGCAAATGCA CCGAATGCTT TACGCACGCC 
GTTGAGGCAG CCGAATTTCT GGCTGCCACG GCGGCCAAAC ATCAGCTGCG GAACGATTTT 
CAGATACACA GTGTTAGGGG CATCAAGAAT CCCGGCGATG AGGACAATGG TGAGCCGCCG 
GCGAATGTGA AATACGTAAT TACTGGCATT ATACTGGTCA TCATTGCATT GGCCTTCTTT 
GGCATGGTCT TGAGTACGCA AAGAAAGCGG GCACATGGCG TCACCTGGTT CCCGGAGGGA 
TTCCGTGCAC CGGCGGCGGT TATGTCGCGA CGTCGACGTG ATCCACACGG CCAAGAGATG 
CGGAATCTCA ACAAGCAGGT GGCCATGCAA TCGCAGGGTG TTGGCCAGCC AGGTGCTCAT 
TGGTCCGACG ATGAATCGGA TATGCCGTTG CCCAAGCGAC AGCGCAGTGA TCCCGTTTCC 
GGTGTCGGTC TGGGCAACAA TGGTGGCTAT GCCAGTGATC ATACAATGGT CAGCGAATAT 
GAGGAGGCTG ATCAGCGGGT CTGGTCGCAG GCCCATCTGG ATGTGGTCGA TGTGCGGGCC 
ATAATGACAC CGCCGGCGCA TCAGGATGGT GGCAAGCACG ATGTGGATGC ACGTGGACCT 
TGCGGACTGA CTCCGCTAAT GATTGCTGCC GTACGAGGCG GTGGCCTGGA CACTGGCGAG 
GATATCGAGA ACAATGAGGA CAGTACTGCC CAGGTGATAT CGGATCTATT GGCACAGGGA 
GCCGAGTTAA ATGCCACAAT GGATAAAACG GGCGAGACAT CATTGCATTT GGCAGCACGT 
TTTGCTCGAG CAGATGCCGC CAAGAGATTA TTGGACGCCG GTGCGGATGC CAATTGTCAG 
GATAACACGG GCAGGACACC GCTGCACGCT GCTGTGGCCG CCGATGCCAT GGGTGTATTT 
CAGATATTGC TTAGGAATAG GGCTACCAAT CTAAATGCTA GAATGCATGA CGGCACAACG 
CCACTAATAC TGGCCGCTCG TCTAGCCATC GAGGGCATGG TGGAGGATTT GATCACAGCC 
GATGCGGATA TTAATGCAGC GGATAATTCC GGCAAGACCG CTCTCCATTG GGCAGCGGCG 
GTTAACAATA CCGAAGCGGT GAACATTCTG CTAATGCATC ATGCCAATCG CGATGCCCAG 
GACGATAAGG ATGAGACACC GCTGTTTCTG GCCGCACGCG AGGGTAGCTA TGAGGCGTGC 
AAGGCGCTGT TGGATAATTT TGCCAATCGC GAGATTACCG ATCACATGGA TCGATTGCCA 
CGCGATGTGG CCAGCGAGCG ACTGCATCAC GATATTGTCA GGCTGCTGGA CGAGCATGTG 
CCGCGATCTC CGCAAATGCT GAGCATGACA CCGCAGGCAA TGATCGGATC CCCGCCGCCG 
GGTCAACAGC AGCCGCAGTT GATCACACAG CCGACGGTCA TTTCCGCCGG AAATGGTGGC 
AACAATGGCA ATGGCAATGC CAGCGGAAAG CAGAGCAACC AAACGGCCAA ACAGAAGGCG 
GCCAAGAAGG CCAAACTAAT CGAGGGCAGT CCGGACAATG GGCTGGATGC CACGGGCAGT 
TTGCGACGCA AGGCAAGTTC AAAAAAGACA AGTGCGGCCT CAAAAAAGGC CGCCAACTTG 
AATGGCCTAA ATCCGGGCCA GCTGACGGGG GGAGTGTCGG GCGTGCCGGG TGTACCGCCA 
ACGAATTCAG CGGCACAAGC AGCTGCAGCG GCAGCGGCAG CCGTGGCGGC CATGTCCCAC 
GAACTGGAGG GTTCGCCGGT GGGCGTGGGC ATGGGCGGGA ATCTGCCCAG TCCGTACGAC 
ACCAGTTCGA TGTACTCGAA TGCGATGGCC GCACCGCTGG CGAACGGCAA TCCGAACACG 
GGCGCCAAGC AGCCGCCGAG CTATGAGGAT TGCATCAAGA ATGCGCAATC GATGCAATCG 
CTGCAGGGCA ATGGTCTGGA TATGATCAAG CTGGATAATT ATGCCTACTC GATGGGCTCG 
CCATTTCAGC AGGAGCTGCT CAATGGCCAA GGACTCGGGA TGAATGGTAA TGGCCAGCGA 
AATGGAGTCG GTCCCGGCGT TCTGCCCGGC GGCCTATGCG GAATGGGCGG TCTGAGTGGA 
GCCGGCAATG GAAATAGCCA CGAACAGGGA CTGAGTCCGC CGTACTCGAA TCAGTCGCCG 
CCGCATTCGG TGCAGAGCAG CCTGGCGCTA TCGCCCCACG CCTACTTGGG CTCGCCATCG 
CCGGCCAAGT CGCGACCCAG TCTACCCACC TCGCCCACCC ACATTCAGGC GATGAGGCAT 
GCCACACAGC AGAAGCAATT CGGTGGCAGT AATCTGAATA GTTTGCTGGG CGGTGCCAAT 
GGCGGGGGCG TGGTCGGCGG AGGAGGAGGT GGTGGTGGTG GTGTTGGCCA GGGGCCACAA 
AACTCACCCG TAAGCTTGGG AATCATCTCG CCGACGGGCA GCGATATGGG CATCATGCTC 
GCCCCGCCCC AATCCTCGAA GAATAGTGCA ATAATGCAAA CGATATCACC CCAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAA CATCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAGC AACAGCAGCA GCAACTCGGA GGCCTGGAGT TCGGTTCAGC GGGCTTGGAC 
CTGAATGGAT TTTGTGGATC TCCGGACTCA TTTCACTCGG GTCAAATGAA TCCGCCCTCG 
ATACAAAGTT CAATGTCCGG CTCATCGCCG TCGACCAATA TGTTGTCGCC GTCGTCGCAG 
CACAATCAGC AGGCCTTCTA CCAGTACCTA ACGCCTTCGA GCCAACATTC CGGCGGCCAT 
ACGCCGCAGC ATTTGGTCCA AACGTTGGAT AGCTATCCGA CGCCCTCGCC GGAGTCACCT 
GGCCACTGGT CCTCCTCGTC GCCGCGATCG AATTCCGATT GGAGCGAGGG CGTTCAGTCG 
CCGGCGGCAA ATAATCTTTA CATATCCGGT GGCCATCAGG CTAACAAGGG TTCCGAGGCC 
ATCTACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

N ( NM_001258581.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245510.1 Drosophila melanogaster notch, transcript variant B (N), mRNA.

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CloneID	OFa00677
Clone ID Related Accession (Same CDS sequence)	NM_057511.4 , NM_001258581.2
Accession Version	NM_001258581.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	notch, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258581.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAATCGC AGCGCAGCCG ACGTCGCAGT CGCGCACCAA ACACTTGGAT TTGTTTTTGG 
ATTAACAAAA TGCACGCCGT TGCGTCGCTG CCGGCGTCGC TGCCTCTGCT GCTGTTGACG 
CTGGCGTTTG CGAATTTGCC AAACACCGTT CGCGGAACTG ATACCGCGTT GGTGGCCGCT 
TCCTGCACAA GTGTCGGTTG CCAGAATGGC GGCACATGCG TTACACAACT CAATGGCAAA 
ACCTACTGCG CATGCGATTC GCACTATGTC GGCGACTACT GTGAACACCG CAATCCGTGC 
AATTCGATGC GTTGCCAAAA TGGTGGCACC TGTCAGGTGA CCTTTCGCAA CGGCCGTCCG 
GGCATCTCGT GCAAGTGTCC TTTGGGCTTC GACGAGTCCC TGTGCGAAAT TGCGGTACCG 
AATGCCTGCG ATCATGTGAC CTGCCTCAAT GGAGGCACCT GTCAGCTAAA GACACTGGAG 
GAGTACACGT GTGCGTGTGC CAATGGCTAT ACAGGTGAAC GATGCGAGAC GAAGAATCTG 
TGCGCAAGTT CTCCCTGCCG GAATGGAGCC ACCTGCACCG CTTTGGCCGG AAGCAGCAGC 
TTCACCTGCT CCTGTCCGCC GGGCTTCACC GGTGACACCT GTTCCTATGA CATCGAGGAG 
TGCCAGTCGA ATCCGTGCAA ATACGGCGGC ACATGTGTCA ACACCCACGG ATCTTACCAG 
TGCATGTGTC CCACGGGCTA CACGGGAAAG GATTGCGACA CCAAGTACAA GCCCTGCTCG 
CCATCGCCGT GCCAGAATGG CGGAATTTGC CGGTCGAACG GTCTGTCCTA CGAGTGCAAG 
TGCCCCAAAG GTTTCGAGGG CAAGAACTGC GAACAGAACT ACGACGATTG CCTGGGACAT 
CTTTGCCAGA ACGGAGGAAC CTGCATCGAT GGCATCTCGG ACTACACCTG CCGCTGTCCA 
CCGAACTTCA CGGGCAGATT CTGCCAGGAC GATGTGGACG AGTGCGCCCA ACGGGATCAT 
CCGGTGTGCC AGAACGGAGC CACCTGTACA AACACTCACG GATCGTACAG CTGCATCTGC 
GTGAATGGCT GGGCGGGCTT GGACTGCAGC AACAACACGG ATGACTGCAA ACAGGCGGCC 
TGTTTCTACG GAGCCACGTG CATCGATGGC GTTGGCAGCT TCTATTGCCA GTGTACGAAG 
GGCAAGACGG GTCTGTTGTG CCACCTGGAC GATGCCTGTA CCTCGAATCC CTGCCATGCG 
GACGCCATTT GTGACACGAG TCCGATAAAC GGCTCCTATG CCTGTTCCTG TGCCACCGGC 
TACAAGGGCG TGGATTGTTC AGAGGACATA GATGAATGCG ATCAGGGATC ACCGTGCGAA 
CACAATGGCA TATGCGTGAA CACACCGGGC AGCTACAGAT GCAATTGCTC CCAGGGCTTT 
ACGGGTCCGC GCTGCGAGAC GAACATCAAT GAGTGCGAAT CGCATCCATG CCAGAACGAG 
GGATCTTGCC TGGATGATCC GGGAACGTTC CGATGCGTCT GTATGCCAGG ATTCACGGGC 
ACACAGTGCG AAATCGACAT TGACGAATGC CAATCGAATC CCTGCTTGAA CGATGGAACT 
TGCCACGACA AGATCAATGG CTTTAAATGC AGTTGTGCCC TGGGCTTTAC GGGCGCACGA 
TGTCAAATCA ATATAGACGA CTGTCAGTCG CAACCATGCC GGAATCGTGG CATCTGTCAC 
GACTCTATAG CGGGCTACAG CTGCGAATGT CCGCCGGGAT ATACGGGCAC CAGTTGCGAG 
ATCAATATCA ACGATTGCGA TTCGAATCCC TGCCATCGGG GCAAGTGCAT CGACGATGTG 
AACAGTTTCA AGTGCCTCTG TGATCCCGGC TACACGGGCT ACATATGCCA GAAGCAGATC 
AACGAATGCG AATCGAATCC GTGCCAGTTC GATGGCCATT GCCAGGATCG TGTGGGCAGC 
TACTATTGTC AGTGCCAGGC GGGAACCAGT GGCAAGAATT GCGAGGTCAA TGTGAACGAA 
TGCCACAGTA ATCCCTGCAA CAATGGTGCC ACCTGCATCG ATGGCATCAA TTCGTACAAG 
TGCCAGTGTG TGCCCGGTTT TACGGGTCAG CACTGCGAGA AGAACGTCGA CGAGTGCATT 
AGCAGTCCGT GTGCCAACAA TGGTGTCTGC ATAGATCAGG TGAATGGCTA CAAGTGCGAG 
TGTCCGCGTG GATTCTACGA TGCCCATTGC CTGAGCGATG TGGACGAGTG CGCATCGAAT 
CCGTGCGTGA ACGAGGGACG TTGCGAGGAT GGAATCAATG AGTTCATCTG CCACTGTCCG 
CCAGGATACA CGGGCAAGCG TTGTGAGCTG GACATAGACG AGTGCAGCAG CAATCCCTGC 
CAGCATGGTG GCACCTGTTA TGATAAGCTC AACGCCTTCA GTTGCCAATG CATGCCGGGC 
TATACGGGCC AAAAGTGCGA AACGAATATT GACGACTGTG TGACCAATCC GTGCGGAAAT 
GGAGGCACCT GCATTGACAA GGTCAACGGC TACAAGTGCG TCTGCAAAGT GCCCTTCACG 
GGCAGGGATT GTGAAAGCAA AATGGATCCC TGCGCCAGCA ATCGTTGCAA AAACGAGGCG 
AAATGCACGC CAAGCTCGAA TTTCCTGGAC TTCTCCTGCA CCTGCAAACT GGGCTATACG 
GGTCGCTATT GCGATGAGGA CATAGACGAA TGCTCACTGA GTTCACCCTG TCGGAATGGA 
GCTAGCTGTT TGAATGTTCC CGGATCGTAT AGGTGCCTCT GCACCAAGGG CTATGAGGGC 
AGAGATTGCG CCATCAATAC GGACGATTGT GCCTCGTTTC CGTGCCAGAA CGGTGGAACC 
TGTCTGGATG GGATCGGTGA CTATAGCTGC CTGTGCGTGG ATGGCTTCGA TGGCAAGCAC 
TGCGAGACGG ACATCAATGA GTGCTTGAGT CAGCCGTGCC AGAATGGAGC CACGTGTAGT 
CAGTATGTGA ATAGCTATAC CTGCACCTGT CCACTCGGAT TCTCCGGCAT CAATTGTCAG 
ACGAACGATG AGGATTGCAC GGAGAGCTCG TGCTTAAACG GTGGCAGTTG CATCGATGGC 
ATCAATGGCT ACAACTGTAG CTGTTTGGCT GGATATTCCG GTGCCAATTG TCAGTACAAG 
TTGAATAAGT GCGATTCGAA TCCCTGTTTG AACGGAGCCA CCTGCCACGA GCAAAACAAC 
GAGTACACAT GCCATTGTCC CAGTGGCTTT ACGGGCAAAC AGTGTTCCGA ATATGTGGAT 
TGGTGTGGTC AATCGCCGTG CGAGAATGGA GCGACCTGTA GCCAGATGAA GCATCAGTTT 
AGCTGCAAAT GCTCGGCAGG ATGGACGGGT AAGCTGTGCG ATGTTCAGAC GATTTCGTGT 
CAGGATGCAG CAGATCGGAA GGGTCTGAGC CTGCGGCAGT TGTGCAACAA TGGCACGTGC 
AAGGATTATG GAAATAGTCA TGTGTGCTAC TGCTCCCAGG GATACGCGGG TAGCTATTGC 
CAAAAGGAGA TCGACGAGTG CCAATCGCAA CCGTGCCAGA ATGGTGGAAC GTGCCGAGAT 
CTCATCGGAG CCTACGAATG CCAGTGTCGC CAGGGATTTC AGGGTCAGAA TTGCGAACTG 
AACATCGATG ACTGTGCACC GAATCCCTGC CAGAATGGCG GCACTTGCCA CGACCGTGTG 
ATGAACTTTA GCTGCAGCTG TCCACCGGGA ACGATGGGCA TCATATGCGA GATCAACAAG 
GATGATTGCA AACCGGGAGC ATGCCACAAC AACGGCAGCT GCATCGATCG CGTTGGAGGC 
TTCGAGTGCG TCTGTCAGCC CGGTTTTGTG GGTGCCCGCT GCGAGGGCGA CATCAACGAG 
TGCCTAAGCA ATCCCTGTTC GAATGCCGGC ACACTGGACT GCGTCCAGTT GGTCAATAAC 
TATCACTGCA ACTGTCGACC TGGACATATG GGTCGGCACT GTGAGCACAA GGTGGACTTT 
TGTGCCCAGA GTCCGTGCCA GAATGGTGGC AATTGTAATA TCCGACAGAG TGGCCACCAC 
TGCATCTGTA ATAATGGATT CTACGGTAAG AACTGTGAGC TGAGTGGTCA GGATTGTGAT 
TCGAATCCCT GCCGTGTGGG TAACTGTGTT GTCGCGGACG AGGGATTCGG CTATAGATGC 
GAGTGTCCAA GGGGCACACT GGGTGAGCAC TGCGAGATTG ATACGCTAGA CGAATGTTCG 
CCGAATCCCT GTGCCCAGGG AGCTGCCTGC GAGGATTTGC TCGGTGATTA CGAATGCCTC 
TGTCCGAGCA AATGGAAAGG CAAACGCTGC GATATCTATG ATGCCAACTA TCCGGGATGG 
AATGGCGGTT CGGGATCCGG AAATGATCGC TATGCCGCTG ATTTGGAGCA ACAACGTGCC 
ATGTGCGATA AGCGAGGTTG CACCGAAAAG CAGGGCAACG GCATTTGCGA TTCCGATTGC 
AATACGTATG CCTGCAACTT TGATGGAAAC GATTGCTCAC TGGGCATCAA TCCGTGGGCC 
AATTGCACGG CCAACGAGTG CTGGAACAAA TTCAAGAACG GCAAATGCAA CGAGGAGTGC 
AACAATGCTG CCTGCCACTA CGATGGTCAC GATTGCGAAC GGAAATTGAA GAGTTGCGAT 
AGCCTTTTCG ATGCCTACTG CCAGAAGCAT TACGGCGATG GTTTCTGCGA CTATGGATGC 
AATAATGCGG AATGCAGTTG GGACGGACTC GACTGTGAGA ACAAGACCCA GTCACCGGTT 
TTGGCCGAGG GTGCCATGTC CGTGGTGATG CTAATGAATG TGGAGGCATT CCGCGAGATT 
CAGGCTCAGT TCCTTAGGAA TATGAGCCAC ATGCTGCGGA CAACGGTGCG GTTGAAGAAG 
GACGCTCTTG GTCATGACAT CATTATCAAT TGGAAGGACA ATGTGCGCGT GCCGGAGATC 
GAGGATACGG ACTTTGCGAG AAAAAACAAG ATTCTGTACA CCCAACAGGT CCATCAGACG 
GGCATTCAAA TCTATTTGGA GATTGACAAT CGCAAATGCA CCGAATGCTT TACGCACGCC 
GTTGAGGCAG CCGAATTTCT GGCTGCCACG GCGGCCAAAC ATCAGCTGCG GAACGATTTT 
CAGATACACA GTGTTAGGGG CATCAAGAAT CCCGGCGATG AGGACAATGG TGAGCCGCCG 
GCGAATGTGA AATACGTAAT TACTGGCATT ATACTGGTCA TCATTGCATT GGCCTTCTTT 
GGCATGGTCT TGAGTACGCA AAGAAAGCGG GCACATGGCG TCACCTGGTT CCCGGAGGGA 
TTCCGTGCAC CGGCGGCGGT TATGTCGCGA CGTCGACGTG ATCCACACGG CCAAGAGATG 
CGGAATCTCA ACAAGCAGGT GGCCATGCAA TCGCAGGGTG TTGGCCAGCC AGGTGCTCAT 
TGGTCCGACG ATGAATCGGA TATGCCGTTG CCCAAGCGAC AGCGCAGTGA TCCCGTTTCC 
GGTGTCGGTC TGGGCAACAA TGGTGGCTAT GCCAGTGATC ATACAATGGT CAGCGAATAT 
GAGGAGGCTG ATCAGCGGGT CTGGTCGCAG GCCCATCTGG ATGTGGTCGA TGTGCGGGCC 
ATAATGACAC CGCCGGCGCA TCAGGATGGT GGCAAGCACG ATGTGGATGC ACGTGGACCT 
TGCGGACTGA CTCCGCTAAT GATTGCTGCC GTACGAGGCG GTGGCCTGGA CACTGGCGAG 
GATATCGAGA ACAATGAGGA CAGTACTGCC CAGGTGATAT CGGATCTATT GGCACAGGGA 
GCCGAGTTAA ATGCCACAAT GGATAAAACG GGCGAGACAT CATTGCATTT GGCAGCACGT 
TTTGCTCGAG CAGATGCCGC CAAGAGATTA TTGGACGCCG GTGCGGATGC CAATTGTCAG 
GATAACACGG GCAGGACACC GCTGCACGCT GCTGTGGCCG CCGATGCCAT GGGTGTATTT 
CAGATATTGC TTAGGAATAG GGCTACCAAT CTAAATGCTA GAATGCATGA CGGCACAACG 
CCACTAATAC TGGCCGCTCG TCTAGCCATC GAGGGCATGG TGGAGGATTT GATCACAGCC 
GATGCGGATA TTAATGCAGC GGATAATTCC GGCAAGACCG CTCTCCATTG GGCAGCGGCG 
GTTAACAATA CCGAAGCGGT GAACATTCTG CTAATGCATC ATGCCAATCG CGATGCCCAG 
GACGATAAGG ATGAGACACC GCTGTTTCTG GCCGCACGCG AGGGTAGCTA TGAGGCGTGC 
AAGGCGCTGT TGGATAATTT TGCCAATCGC GAGATTACCG ATCACATGGA TCGATTGCCA 
CGCGATGTGG CCAGCGAGCG ACTGCATCAC GATATTGTCA GGCTGCTGGA CGAGCATGTG 
CCGCGATCTC CGCAAATGCT GAGCATGACA CCGCAGGCAA TGATCGGATC CCCGCCGCCG 
GGTCAACAGC AGCCGCAGTT GATCACACAG CCGACGGTCA TTTCCGCCGG AAATGGTGGC 
AACAATGGCA ATGGCAATGC CAGCGGAAAG CAGAGCAACC AAACGGCCAA ACAGAAGGCG 
GCCAAGAAGG CCAAACTAAT CGAGGGCAGT CCGGACAATG GGCTGGATGC CACGGGCAGT 
TTGCGACGCA AGGCAAGTTC AAAAAAGACA AGTGCGGCCT CAAAAAAGGC CGCCAACTTG 
AATGGCCTAA ATCCGGGCCA GCTGACGGGG GGAGTGTCGG GCGTGCCGGG TGTACCGCCA 
ACGAATTCAG CGGCACAAGC AGCTGCAGCG GCAGCGGCAG CCGTGGCGGC CATGTCCCAC 
GAACTGGAGG GTTCGCCGGT GGGCGTGGGC ATGGGCGGGA ATCTGCCCAG TCCGTACGAC 
ACCAGTTCGA TGTACTCGAA TGCGATGGCC GCACCGCTGG CGAACGGCAA TCCGAACACG 
GGCGCCAAGC AGCCGCCGAG CTATGAGGAT TGCATCAAGA ATGCGCAATC GATGCAATCG 
CTGCAGGGCA ATGGTCTGGA TATGATCAAG CTGGATAATT ATGCCTACTC GATGGGCTCG 
CCATTTCAGC AGGAGCTGCT CAATGGCCAA GGACTCGGGA TGAATGGTAA TGGCCAGCGA 
AATGGAGTCG GTCCCGGCGT TCTGCCCGGC GGCCTATGCG GAATGGGCGG TCTGAGTGGA 
GCCGGCAATG GAAATAGCCA CGAACAGGGA CTGAGTCCGC CGTACTCGAA TCAGTCGCCG 
CCGCATTCGG TGCAGAGCAG CCTGGCGCTA TCGCCCCACG CCTACTTGGG CTCGCCATCG 
CCGGCCAAGT CGCGACCCAG TCTACCCACC TCGCCCACCC ACATTCAGGC GATGAGGCAT 
GCCACACAGC AGAAGCAATT CGGTGGCAGT AATCTGAATA GTTTGCTGGG CGGTGCCAAT 
GGCGGGGGCG TGGTCGGCGG AGGAGGAGGT GGTGGTGGTG GTGTTGGCCA GGGGCCACAA 
AACTCACCCG TAAGCTTGGG AATCATCTCG CCGACGGGCA GCGATATGGG CATCATGCTC 
GCCCCGCCCC AATCCTCGAA GAATAGTGCA ATAATGCAAA CGATATCACC CCAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAA CATCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAGC AACAGCAGCA GCAACTCGGA GGCCTGGAGT TCGGTTCAGC GGGCTTGGAC 
CTGAATGGAT TTTGTGGATC TCCGGACTCA TTTCACTCGG GTCAAATGAA TCCGCCCTCG 
ATACAAAGTT CAATGTCCGG CTCATCGCCG TCGACCAATA TGTTGTCGCC GTCGTCGCAG 
CACAATCAGC AGGCCTTCTA CCAGTACCTA ACGCCTTCGA GCCAACATTC CGGCGGCCAT 
ACGCCGCAGC ATTTGGTCCA AACGTTGGAT AGCTATCCGA CGCCCTCGCC GGAGTCACCT 
GGCCACTGGT CCTCCTCGTC GCCGCGATCG AATTCCGATT GGAGCGAGGG CGTTCAGTCG 
CCGGCGGCAA ATAATCTTTA CATATCCGGT GGCCATCAGG CTAACAAGGG TTCCGAGGCC 
ATCTACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245510.1
CDS	799..8910
Translation	MQSQRSRRRSRAPNTWICFWINKMHAVASLPASLPLLLLTLAFANLPNTVRGTDTALVAASCTSVGCQNGGTCVTQLNGKTYCACDSHYVGDYCEHRNPCNSMRCQNGGTCQVTFRNGRPGISCKCPLGFDESLCEIAVPNACDHVTCLNGGTCQLKTLEEYTCACANGYTGERCETKNLCASSPCRNGATCTALAGSSSFTCSCPPGFTGDTCSYDIEECQSNPCKYGGTCVNTHGSYQCMCPTGYTGKDCDTKYKPCSPSPCQNGGICRSNGLSYECKCPKGFEGKNCEQNYDDCLGHLCQNGGTCIDGISDYTCRCPPNFTGRFCQDDVDECAQRDHPVCQNGATCTNTHGSYSCICVNGWAGLDCSNNTDDCKQAACFYGATCIDGVGSFYCQCTKGKTGLLCHLDDACTSNPCHADAICDTSPINGSYACSCATGYKGVDCSEDIDECDQGSPCEHNGICVNTPGSYRCNCSQGFTGPRCETNINECESHPCQNEGSCLDDPGTFRCVCMPGFTGTQCEIDIDECQSNPCLNDGTCHDKINGFKCSCALGFTGARCQINIDDCQSQPCRNRGICHDSIAGYSCECPPGYTGTSCEININDCDSNPCHRGKCIDDVNSFKCLCDPGYTGYICQKQINECESNPCQFDGHCQDRVGSYYCQCQAGTSGKNCEVNVNECHSNPCNNGATCIDGINSYKCQCVPGFTGQHCEKNVDECISSPCANNGVCIDQVNGYKCECPRGFYDAHCLSDVDECASNPCVNEGRCEDGINEFICHCPPGYTGKRCELDIDECSSNPCQHGGTCYDKLNAFSCQCMPGYTGQKCETNIDDCVTNPCGNGGTCIDKVNGYKCVCKVPFTGRDCESKMDPCASNRCKNEAKCTPSSNFLDFSCTCKLGYTGRYCDEDIDECSLSSPCRNGASCLNVPGSYRCLCTKGYEGRDCAINTDDCASFPCQNGGTCLDGIGDYSCLCVDGFDGKHCETDINECLSQPCQNGATCSQYVNSYTCTCPLGFSGINCQTNDEDCTESSCLNGGSCIDGINGYNCSCLAGYSGANCQYKLNKCDSNPCLNGATCHEQNNEYTCHCPSGFTGKQCSEYVDWCGQSPCENGATCSQMKHQFSCKCSAGWTGKLCDVQTISCQDAADRKGLSLRQLCNNGTCKDYGNSHVCYCSQGYAGSYCQKEIDECQSQPCQNGGTCRDLIGAYECQCRQGFQGQNCELNIDDCAPNPCQNGGTCHDRVMNFSCSCPPGTMGIICEINKDDCKPGACHNNGSCIDRVGGFECVCQPGFVGARCEGDINECLSNPCSNAGTLDCVQLVNNYHCNCRPGHMGRHCEHKVDFCAQSPCQNGGNCNIRQSGHHCICNNGFYGKNCELSGQDCDSNPCRVGNCVVADEGFGYRCECPRGTLGEHCEIDTLDECSPNPCAQGAACEDLLGDYECLCPSKWKGKRCDIYDANYPGWNGGSGSGNDRYAADLEQQRAMCDKRGCTEKQGNGICDSDCNTYACNFDGNDCSLGINPWANCTANECWNKFKNGKCNEECNNAACHYDGHDCERKLKSCDSLFDAYCQKHYGDGFCDYGCNNAECSWDGLDCENKTQSPVLAEGAMSVVMLMNVEAFREIQAQFLRNMSHMLRTTVRLKKDALGHDIIINWKDNVRVPEIEDTDFARKNKILYTQQVHQTGIQIYLEIDNRKCTECFTHAVEAAEFLAATAAKHQLRNDFQIHSVRGIKNPGDEDNGEPPANVKYVITGIILVIIALAFFGMVLSTQRKRAHGVTWFPEGFRAPAAVMSRRRRDPHGQEMRNLNKQVAMQSQGVGQPGAHWSDDESDMPLPKRQRSDPVSGVGLGNNGGYASDHTMVSEYEEADQRVWSQAHLDVVDVRAIMTPPAHQDGGKHDVDARGPCGLTPLMIAAVRGGGLDTGEDIENNEDSTAQVISDLLAQGAELNATMDKTGETSLHLAARFARADAAKRLLDAGADANCQDNTGRTPLHAAVAADAMGVFQILLRNRATNLNARMHDGTTPLILAARLAIEGMVEDLITADADINAADNSGKTALHWAAAVNNTEAVNILLMHHANRDAQDDKDETPLFLAAREGSYEACKALLDNFANREITDHMDRLPRDVASERLHHDIVRLLDEHVPRSPQMLSMTPQAMIGSPPPGQQQPQLITQPTVISAGNGGNNGNGNASGKQSNQTAKQKAAKKAKLIEGSPDNGLDATGSLRRKASSKKTSAASKKAANLNGLNPGQLTGGVSGVPGVPPTNSAAQAAAAAAAAVAAMSHELEGSPVGVGMGGNLPSPYDTSSMYSNAMAAPLANGNPNTGAKQPPSYEDCIKNAQSMQSLQGNGLDMIKLDNYAYSMGSPFQQELLNGQGLGMNGNGQRNGVGPGVLPGGLCGMGGLSGAGNGNSHEQGLSPPYSNQSPPHSVQSSLALSPHAYLGSPSPAKSRPSLPTSPTHIQAMRHATQQKQFGGSNLNSLLGGANGGGVVGGGGGGGGGVGQGPQNSPVSLGIISPTGSDMGIMLAPPQSSKNSAIMQTISPQQQQQQQQQQQQQHQQQQQQQQQQQQQQQQQLGGLEFGSAGLDLNGFCGSPDSFHSGQMNPPSIQSSMSGSSPSTNMLSPSSQHNQQAFYQYLTPSSQHSGGHTPQHLVQTLDSYPTPSPESPGHWSSSSPRSNSDWSEGVQSPAANNLYISGGHQANKGSEAIYI

Target ORF information:

RefSeq Version	NM_001258581.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster notch, transcript variant B (N), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258581.2

ORF Insert Sequence:

ATGCAATCGC AGCGCAGCCG ACGTCGCAGT CGCGCACCAA ACACTTGGAT TTGTTTTTGG 
ATTAACAAAA TGCACGCCGT TGCGTCGCTG CCGGCGTCGC TGCCTCTGCT GCTGTTGACG 
CTGGCGTTTG CGAATTTGCC AAACACCGTT CGCGGAACTG ATACCGCGTT GGTGGCCGCT 
TCCTGCACAA GTGTCGGTTG CCAGAATGGC GGCACATGCG TTACACAACT CAATGGCAAA 
ACCTACTGCG CATGCGATTC GCACTATGTC GGCGACTACT GTGAACACCG CAATCCGTGC 
AATTCGATGC GTTGCCAAAA TGGTGGCACC TGTCAGGTGA CCTTTCGCAA CGGCCGTCCG 
GGCATCTCGT GCAAGTGTCC TTTGGGCTTC GACGAGTCCC TGTGCGAAAT TGCGGTACCG 
AATGCCTGCG ATCATGTGAC CTGCCTCAAT GGAGGCACCT GTCAGCTAAA GACACTGGAG 
GAGTACACGT GTGCGTGTGC CAATGGCTAT ACAGGTGAAC GATGCGAGAC GAAGAATCTG 
TGCGCAAGTT CTCCCTGCCG GAATGGAGCC ACCTGCACCG CTTTGGCCGG AAGCAGCAGC 
TTCACCTGCT CCTGTCCGCC GGGCTTCACC GGTGACACCT GTTCCTATGA CATCGAGGAG 
TGCCAGTCGA ATCCGTGCAA ATACGGCGGC ACATGTGTCA ACACCCACGG ATCTTACCAG 
TGCATGTGTC CCACGGGCTA CACGGGAAAG GATTGCGACA CCAAGTACAA GCCCTGCTCG 
CCATCGCCGT GCCAGAATGG CGGAATTTGC CGGTCGAACG GTCTGTCCTA CGAGTGCAAG 
TGCCCCAAAG GTTTCGAGGG CAAGAACTGC GAACAGAACT ACGACGATTG CCTGGGACAT 
CTTTGCCAGA ACGGAGGAAC CTGCATCGAT GGCATCTCGG ACTACACCTG CCGCTGTCCA 
CCGAACTTCA CGGGCAGATT CTGCCAGGAC GATGTGGACG AGTGCGCCCA ACGGGATCAT 
CCGGTGTGCC AGAACGGAGC CACCTGTACA AACACTCACG GATCGTACAG CTGCATCTGC 
GTGAATGGCT GGGCGGGCTT GGACTGCAGC AACAACACGG ATGACTGCAA ACAGGCGGCC 
TGTTTCTACG GAGCCACGTG CATCGATGGC GTTGGCAGCT TCTATTGCCA GTGTACGAAG 
GGCAAGACGG GTCTGTTGTG CCACCTGGAC GATGCCTGTA CCTCGAATCC CTGCCATGCG 
GACGCCATTT GTGACACGAG TCCGATAAAC GGCTCCTATG CCTGTTCCTG TGCCACCGGC 
TACAAGGGCG TGGATTGTTC AGAGGACATA GATGAATGCG ATCAGGGATC ACCGTGCGAA 
CACAATGGCA TATGCGTGAA CACACCGGGC AGCTACAGAT GCAATTGCTC CCAGGGCTTT 
ACGGGTCCGC GCTGCGAGAC GAACATCAAT GAGTGCGAAT CGCATCCATG CCAGAACGAG 
GGATCTTGCC TGGATGATCC GGGAACGTTC CGATGCGTCT GTATGCCAGG ATTCACGGGC 
ACACAGTGCG AAATCGACAT TGACGAATGC CAATCGAATC CCTGCTTGAA CGATGGAACT 
TGCCACGACA AGATCAATGG CTTTAAATGC AGTTGTGCCC TGGGCTTTAC GGGCGCACGA 
TGTCAAATCA ATATAGACGA CTGTCAGTCG CAACCATGCC GGAATCGTGG CATCTGTCAC 
GACTCTATAG CGGGCTACAG CTGCGAATGT CCGCCGGGAT ATACGGGCAC CAGTTGCGAG 
ATCAATATCA ACGATTGCGA TTCGAATCCC TGCCATCGGG GCAAGTGCAT CGACGATGTG 
AACAGTTTCA AGTGCCTCTG TGATCCCGGC TACACGGGCT ACATATGCCA GAAGCAGATC 
AACGAATGCG AATCGAATCC GTGCCAGTTC GATGGCCATT GCCAGGATCG TGTGGGCAGC 
TACTATTGTC AGTGCCAGGC GGGAACCAGT GGCAAGAATT GCGAGGTCAA TGTGAACGAA 
TGCCACAGTA ATCCCTGCAA CAATGGTGCC ACCTGCATCG ATGGCATCAA TTCGTACAAG 
TGCCAGTGTG TGCCCGGTTT TACGGGTCAG CACTGCGAGA AGAACGTCGA CGAGTGCATT 
AGCAGTCCGT GTGCCAACAA TGGTGTCTGC ATAGATCAGG TGAATGGCTA CAAGTGCGAG 
TGTCCGCGTG GATTCTACGA TGCCCATTGC CTGAGCGATG TGGACGAGTG CGCATCGAAT 
CCGTGCGTGA ACGAGGGACG TTGCGAGGAT GGAATCAATG AGTTCATCTG CCACTGTCCG 
CCAGGATACA CGGGCAAGCG TTGTGAGCTG GACATAGACG AGTGCAGCAG CAATCCCTGC 
CAGCATGGTG GCACCTGTTA TGATAAGCTC AACGCCTTCA GTTGCCAATG CATGCCGGGC 
TATACGGGCC AAAAGTGCGA AACGAATATT GACGACTGTG TGACCAATCC GTGCGGAAAT 
GGAGGCACCT GCATTGACAA GGTCAACGGC TACAAGTGCG TCTGCAAAGT GCCCTTCACG 
GGCAGGGATT GTGAAAGCAA AATGGATCCC TGCGCCAGCA ATCGTTGCAA AAACGAGGCG 
AAATGCACGC CAAGCTCGAA TTTCCTGGAC TTCTCCTGCA CCTGCAAACT GGGCTATACG 
GGTCGCTATT GCGATGAGGA CATAGACGAA TGCTCACTGA GTTCACCCTG TCGGAATGGA 
GCTAGCTGTT TGAATGTTCC CGGATCGTAT AGGTGCCTCT GCACCAAGGG CTATGAGGGC 
AGAGATTGCG CCATCAATAC GGACGATTGT GCCTCGTTTC CGTGCCAGAA CGGTGGAACC 
TGTCTGGATG GGATCGGTGA CTATAGCTGC CTGTGCGTGG ATGGCTTCGA TGGCAAGCAC 
TGCGAGACGG ACATCAATGA GTGCTTGAGT CAGCCGTGCC AGAATGGAGC CACGTGTAGT 
CAGTATGTGA ATAGCTATAC CTGCACCTGT CCACTCGGAT TCTCCGGCAT CAATTGTCAG 
ACGAACGATG AGGATTGCAC GGAGAGCTCG TGCTTAAACG GTGGCAGTTG CATCGATGGC 
ATCAATGGCT ACAACTGTAG CTGTTTGGCT GGATATTCCG GTGCCAATTG TCAGTACAAG 
TTGAATAAGT GCGATTCGAA TCCCTGTTTG AACGGAGCCA CCTGCCACGA GCAAAACAAC 
GAGTACACAT GCCATTGTCC CAGTGGCTTT ACGGGCAAAC AGTGTTCCGA ATATGTGGAT 
TGGTGTGGTC AATCGCCGTG CGAGAATGGA GCGACCTGTA GCCAGATGAA GCATCAGTTT 
AGCTGCAAAT GCTCGGCAGG ATGGACGGGT AAGCTGTGCG ATGTTCAGAC GATTTCGTGT 
CAGGATGCAG CAGATCGGAA GGGTCTGAGC CTGCGGCAGT TGTGCAACAA TGGCACGTGC 
AAGGATTATG GAAATAGTCA TGTGTGCTAC TGCTCCCAGG GATACGCGGG TAGCTATTGC 
CAAAAGGAGA TCGACGAGTG CCAATCGCAA CCGTGCCAGA ATGGTGGAAC GTGCCGAGAT 
CTCATCGGAG CCTACGAATG CCAGTGTCGC CAGGGATTTC AGGGTCAGAA TTGCGAACTG 
AACATCGATG ACTGTGCACC GAATCCCTGC CAGAATGGCG GCACTTGCCA CGACCGTGTG 
ATGAACTTTA GCTGCAGCTG TCCACCGGGA ACGATGGGCA TCATATGCGA GATCAACAAG 
GATGATTGCA AACCGGGAGC ATGCCACAAC AACGGCAGCT GCATCGATCG CGTTGGAGGC 
TTCGAGTGCG TCTGTCAGCC CGGTTTTGTG GGTGCCCGCT GCGAGGGCGA CATCAACGAG 
TGCCTAAGCA ATCCCTGTTC GAATGCCGGC ACACTGGACT GCGTCCAGTT GGTCAATAAC 
TATCACTGCA ACTGTCGACC TGGACATATG GGTCGGCACT GTGAGCACAA GGTGGACTTT 
TGTGCCCAGA GTCCGTGCCA GAATGGTGGC AATTGTAATA TCCGACAGAG TGGCCACCAC 
TGCATCTGTA ATAATGGATT CTACGGTAAG AACTGTGAGC TGAGTGGTCA GGATTGTGAT 
TCGAATCCCT GCCGTGTGGG TAACTGTGTT GTCGCGGACG AGGGATTCGG CTATAGATGC 
GAGTGTCCAA GGGGCACACT GGGTGAGCAC TGCGAGATTG ATACGCTAGA CGAATGTTCG 
CCGAATCCCT GTGCCCAGGG AGCTGCCTGC GAGGATTTGC TCGGTGATTA CGAATGCCTC 
TGTCCGAGCA AATGGAAAGG CAAACGCTGC GATATCTATG ATGCCAACTA TCCGGGATGG 
AATGGCGGTT CGGGATCCGG AAATGATCGC TATGCCGCTG ATTTGGAGCA ACAACGTGCC 
ATGTGCGATA AGCGAGGTTG CACCGAAAAG CAGGGCAACG GCATTTGCGA TTCCGATTGC 
AATACGTATG CCTGCAACTT TGATGGAAAC GATTGCTCAC TGGGCATCAA TCCGTGGGCC 
AATTGCACGG CCAACGAGTG CTGGAACAAA TTCAAGAACG GCAAATGCAA CGAGGAGTGC 
AACAATGCTG CCTGCCACTA CGATGGTCAC GATTGCGAAC GGAAATTGAA GAGTTGCGAT 
AGCCTTTTCG ATGCCTACTG CCAGAAGCAT TACGGCGATG GTTTCTGCGA CTATGGATGC 
AATAATGCGG AATGCAGTTG GGACGGACTC GACTGTGAGA ACAAGACCCA GTCACCGGTT 
TTGGCCGAGG GTGCCATGTC CGTGGTGATG CTAATGAATG TGGAGGCATT CCGCGAGATT 
CAGGCTCAGT TCCTTAGGAA TATGAGCCAC ATGCTGCGGA CAACGGTGCG GTTGAAGAAG 
GACGCTCTTG GTCATGACAT CATTATCAAT TGGAAGGACA ATGTGCGCGT GCCGGAGATC 
GAGGATACGG ACTTTGCGAG AAAAAACAAG ATTCTGTACA CCCAACAGGT CCATCAGACG 
GGCATTCAAA TCTATTTGGA GATTGACAAT CGCAAATGCA CCGAATGCTT TACGCACGCC 
GTTGAGGCAG CCGAATTTCT GGCTGCCACG GCGGCCAAAC ATCAGCTGCG GAACGATTTT 
CAGATACACA GTGTTAGGGG CATCAAGAAT CCCGGCGATG AGGACAATGG TGAGCCGCCG 
GCGAATGTGA AATACGTAAT TACTGGCATT ATACTGGTCA TCATTGCATT GGCCTTCTTT 
GGCATGGTCT TGAGTACGCA AAGAAAGCGG GCACATGGCG TCACCTGGTT CCCGGAGGGA 
TTCCGTGCAC CGGCGGCGGT TATGTCGCGA CGTCGACGTG ATCCACACGG CCAAGAGATG 
CGGAATCTCA ACAAGCAGGT GGCCATGCAA TCGCAGGGTG TTGGCCAGCC AGGTGCTCAT 
TGGTCCGACG ATGAATCGGA TATGCCGTTG CCCAAGCGAC AGCGCAGTGA TCCCGTTTCC 
GGTGTCGGTC TGGGCAACAA TGGTGGCTAT GCCAGTGATC ATACAATGGT CAGCGAATAT 
GAGGAGGCTG ATCAGCGGGT CTGGTCGCAG GCCCATCTGG ATGTGGTCGA TGTGCGGGCC 
ATAATGACAC CGCCGGCGCA TCAGGATGGT GGCAAGCACG ATGTGGATGC ACGTGGACCT 
TGCGGACTGA CTCCGCTAAT GATTGCTGCC GTACGAGGCG GTGGCCTGGA CACTGGCGAG 
GATATCGAGA ACAATGAGGA CAGTACTGCC CAGGTGATAT CGGATCTATT GGCACAGGGA 
GCCGAGTTAA ATGCCACAAT GGATAAAACG GGCGAGACAT CATTGCATTT GGCAGCACGT 
TTTGCTCGAG CAGATGCCGC CAAGAGATTA TTGGACGCCG GTGCGGATGC CAATTGTCAG 
GATAACACGG GCAGGACACC GCTGCACGCT GCTGTGGCCG CCGATGCCAT GGGTGTATTT 
CAGATATTGC TTAGGAATAG GGCTACCAAT CTAAATGCTA GAATGCATGA CGGCACAACG 
CCACTAATAC TGGCCGCTCG TCTAGCCATC GAGGGCATGG TGGAGGATTT GATCACAGCC 
GATGCGGATA TTAATGCAGC GGATAATTCC GGCAAGACCG CTCTCCATTG GGCAGCGGCG 
GTTAACAATA CCGAAGCGGT GAACATTCTG CTAATGCATC ATGCCAATCG CGATGCCCAG 
GACGATAAGG ATGAGACACC GCTGTTTCTG GCCGCACGCG AGGGTAGCTA TGAGGCGTGC 
AAGGCGCTGT TGGATAATTT TGCCAATCGC GAGATTACCG ATCACATGGA TCGATTGCCA 
CGCGATGTGG CCAGCGAGCG ACTGCATCAC GATATTGTCA GGCTGCTGGA CGAGCATGTG 
CCGCGATCTC CGCAAATGCT GAGCATGACA CCGCAGGCAA TGATCGGATC CCCGCCGCCG 
GGTCAACAGC AGCCGCAGTT GATCACACAG CCGACGGTCA TTTCCGCCGG AAATGGTGGC 
AACAATGGCA ATGGCAATGC CAGCGGAAAG CAGAGCAACC AAACGGCCAA ACAGAAGGCG 
GCCAAGAAGG CCAAACTAAT CGAGGGCAGT CCGGACAATG GGCTGGATGC CACGGGCAGT 
TTGCGACGCA AGGCAAGTTC AAAAAAGACA AGTGCGGCCT CAAAAAAGGC CGCCAACTTG 
AATGGCCTAA ATCCGGGCCA GCTGACGGGG GGAGTGTCGG GCGTGCCGGG TGTACCGCCA 
ACGAATTCAG CGGCACAAGC AGCTGCAGCG GCAGCGGCAG CCGTGGCGGC CATGTCCCAC 
GAACTGGAGG GTTCGCCGGT GGGCGTGGGC ATGGGCGGGA ATCTGCCCAG TCCGTACGAC 
ACCAGTTCGA TGTACTCGAA TGCGATGGCC GCACCGCTGG CGAACGGCAA TCCGAACACG 
GGCGCCAAGC AGCCGCCGAG CTATGAGGAT TGCATCAAGA ATGCGCAATC GATGCAATCG 
CTGCAGGGCA ATGGTCTGGA TATGATCAAG CTGGATAATT ATGCCTACTC GATGGGCTCG 
CCATTTCAGC AGGAGCTGCT CAATGGCCAA GGACTCGGGA TGAATGGTAA TGGCCAGCGA 
AATGGAGTCG GTCCCGGCGT TCTGCCCGGC GGCCTATGCG GAATGGGCGG TCTGAGTGGA 
GCCGGCAATG GAAATAGCCA CGAACAGGGA CTGAGTCCGC CGTACTCGAA TCAGTCGCCG 
CCGCATTCGG TGCAGAGCAG CCTGGCGCTA TCGCCCCACG CCTACTTGGG CTCGCCATCG 
CCGGCCAAGT CGCGACCCAG TCTACCCACC TCGCCCACCC ACATTCAGGC GATGAGGCAT 
GCCACACAGC AGAAGCAATT CGGTGGCAGT AATCTGAATA GTTTGCTGGG CGGTGCCAAT 
GGCGGGGGCG TGGTCGGCGG AGGAGGAGGT GGTGGTGGTG GTGTTGGCCA GGGGCCACAA 
AACTCACCCG TAAGCTTGGG AATCATCTCG CCGACGGGCA GCGATATGGG CATCATGCTC 
GCCCCGCCCC AATCCTCGAA GAATAGTGCA ATAATGCAAA CGATATCACC CCAGCAACAG 
CAGCAGCAGC AGCAACAGCA ACAGCAGCAA CATCAGCAGC AGCAACAGCA GCAGCAACAG 
CAGCAGCAGC AACAGCAGCA GCAACTCGGA GGCCTGGAGT TCGGTTCAGC GGGCTTGGAC 
CTGAATGGAT TTTGTGGATC TCCGGACTCA TTTCACTCGG GTCAAATGAA TCCGCCCTCG 
ATACAAAGTT CAATGTCCGG CTCATCGCCG TCGACCAATA TGTTGTCGCC GTCGTCGCAG 
CACAATCAGC AGGCCTTCTA CCAGTACCTA ACGCCTTCGA GCCAACATTC CGGCGGCCAT 
ACGCCGCAGC ATTTGGTCCA AACGTTGGAT AGCTATCCGA CGCCCTCGCC GGAGTCACCT 
GGCCACTGGT CCTCCTCGTC GCCGCGATCG AATTCCGATT GGAGCGAGGG CGTTCAGTCG 
CCGGCGGCAA ATAATCTTTA CATATCCGGT GGCCATCAGG CTAACAAGGG TTCCGAGGCC 
ATCTACATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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N cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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