w cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	w
Entrez Gene ID	31271
Full Name	white
Synonyms	BACN33B1.1,CG2759,DMWHITE,Dmel\CG2759,EG:BACN33B1.1,Hid,W,c23,e(g),m(g),mw,w(AT)[[13]]
General protein information	Preferred Names white Names CG2759 gene product from transcript CG2759-RA CG2759-PA enhancer of garnet mini-white modifier of garnet w-PA white[+]
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3B6-3B6\|1-1.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057439.2	NP_476787.1	white

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCG2	NP_004818.2
Pan troglodytes (chimpanzee)	ABCG2	XP_526633.3
Canis lupus familiaris (dog)	ABCG2	NP_001041486.1
Gallus gallus (chicken)	ABCG2	XP_421638.4
Anopheles gambiae (African malaria mosquito)	WHITE_ANOGA	XP_310530.3
Caenorhabditis elegans (roundworm)	wht-7	NP_001040881.1
Oryza sativa (rice)	Os03g0157400	NP_001049014.1
	ABCG2	NP_001028091.1
Bos taurus (cattle)	ABCG2	NP_001032555.2
Rattus norvegicus (Norway rat)	Abcg2	NP_852046.1
Drosophila melanogaster (fruit fly)	w	NP_476787.1
Caenorhabditis elegans (roundworm)	wht-4	NP_494495.2
Kluyveromyces lactis	KLLA0D04554g	XP_453265.1
Arabidopsis thaliana (thale cress)	AT5G06530	NP_850781.2
Xenopus tropicalis (tropical clawed frog)	abcg2	NP_001039227.1
Mus musculus (house mouse)	Abcg2	NP_036050.1
Saccharomyces cerevisiae (baker's yeast)	ADP1	NP_009937.2
Eremothecium gossypii	AGOS_AER190W	NP_985047.1
Caenorhabditis elegans (roundworm)	wht-1	NP_498322.1
Neurospora crassa	NCU02544	XP_965675.1
Danio rerio (zebrafish)	abcg2a	NP_001036240.1
Magnaporthe oryzae (rice blast fungus)	MGG_01563	XP_003714538.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that age-dependent behavioral/physiological changes differ between wide-type flies (Canton-S flies) and genetically engineered flies null mutation of white gene (w1118 flies); old w1118 flies exhibit decreased path length per minute and reduced 0.2 s path increment compared with young flies, whereas old Canton-S flies exhibit same path length per minute and same 0.2 s path increment compared with young flies.
Title: Different age-dependent performance in Drosophila wild-type Canton-S and the white mutant w1118 flies.

data suggest that a White-cGMP interaction modulates the timing of locomotor recovery from anoxia
Title: White - cGMP Interaction Promotes Fast Locomotor Recovery from Anoxia in Adult Drosophila.

White modulates the timing of locomotor recovery from anoxia.
Title: Timing of Locomotor Recovery from Anoxia Modulated by the white Gene in Drosophila.

the negative effect of transcription of the Drosophila genes white and yellow remained, but weakened with increased distance between the leading promoter and enhancer and with decreased distance between the enhancer and target promoter
Title: [The Effect of Transcription on Enhancer Activity in Drosophila melanogaster].

this study we have used the CRISPR/Cas9 system to introduce site-specific mutations in the D. suzukii white (w) and Sex lethal (Sxl) genes.
Title: CRISPR/Cas9-mediated mutagenesis of the white and Sex lethal loci in the invasive pest, Drosophila suzukii.

The following w gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the w cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00653	NM_057439.2 Latest version!	Drosophila melanogaster white (w), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

w ( NM_057439.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476787.1 Drosophila melanogaster white (w), mRNA.

Price & Availability↑

CloneID	OFa00653
Clone ID Related Accession (Same CDS sequence)	NM_057439.2
Accession Version	NM_057439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	white
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Mar 19, 2002 this sequence version replaced NM_057439.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCCAAG AGGATCAGGA GCTATTAATT CGCGGAGGCA GCAAACACCC ATCTGCCGAG 
CATCTGAACA ATGGTGACAG CGGAGCGGCT TCGCAGAGCT GCATTAACCA GGGCTTCGGG 
CAGGCCAAAA ACTACGGCAC GCTCCGGCCA CCCAGTCCGC CGGAGGACTC CGGTTCAGGG 
AGCGGCCAAC TAGCCGAGAA CCTCACCTAT GCCTGGCACA ATATGGACAT CTTTGGGGCG 
GTCAATCAGC CGGGCTCCGG ATGGCGGCAG CTGGTCAACC GGACACGCGG ACTATTCTGC 
AACGAGCGAC ACATACCGGC GCCCAGGAAA CATTTGCTCA AGAACGTTTG CGGCGTGGCC 
TATCCGGGCG AACTTTTGGC CGTGATGGGC AGTTCCGGTG CCGGAAAGAC GACCCTGCTG 
AATGCCCTTG CCTTTCGATC GCCGCAGGGC ATCCAAGTAT CGCCATCCGG GATGCGACTG 
CTCAATGGCC AACCTGTGGA CGCCAAGGAG ATGCAGGCCA GGTGCGCCTA TGTCCAGCAG 
GATGACCTCT TTATCGGCTC CCTAACGGCC AGGGAACACC TGATTTTCCA AGCCATGGTG 
CGGATGCCAC GACATCTGAC CTATCGGCAG CGAGTGGCCC GCGTGGATCA GGTGATCCAG 
GAGCTTTCGC TCAGCAAATG TCAGCACACG ATCATCGGTG TGCCCGGCAG GGTGAAAGGT 
CTGTCCGGCG GAGAAAGGAA GCGTCTGGCA TTCGCCTCCG AGGCTCTAAC CGATCCGCCG 
CTTCTGATCT GCGATGAGCC CACCTCCGGA CTGGACTCCT TTACCGCCCA CAGCGTCGTC 
CAGGTGCTGA AGAAGCTGTC GCAGAAGGGC AAGACCGTCA TCCTGACCAT TCATCAGCCG 
TCTTCCGAGC TGTTTGAGCT CTTTGACAAG ATCCTTCTGA TGGCCGAGGG CAGGGTAGCT 
TTCTTGGGCA CTCCCAGCGA AGCCGTCGAC TTCTTTTCCT ACGTGGGTGC CCAGTGTCCT 
ACCAACTACA ATCCGGCGGA CTTTTACGTA CAGGTGTTGG CCGTTGTGCC CGGACGGGAG 
ATCGAGTCCC GTGATCGGAT CGCCAAGATA TGCGACAATT TTGCCATTAG CAAAGTAGCC 
CGGGATATGG AGCAGTTGTT GGCCACCAAA AATCTGGAGA AGCCACTGGA GCAGCCGGAG 
AATGGGTACA CCTACAAGGC CACCTGGTTC ATGCAGTTCC GGGCGGTCCT GTGGCGATCC 
TGGCTGTCGG TGCTCAAGGA ACCACTCCTC GTAAAAGTGC GACTTATTCA GACAACGATG 
GTTGCCATCT TGATTGGCCT CATCTTTTTG GGCCAACAAC TCACGCAAGT GGGTGTGATG 
AATATCAACG GAGCCATCTT CCTCTTCCTG ACCAACATGA CCTTTCAAAA CGTCTTTGCC 
ACGATAAATG TGTTCACCTC AGAGCTGCCA GTTTTTATGA GGGAGGCCCG AAGTCGACTT 
TATCGCTGTG ACACATACTT TCTGGGCAAA ACGATTGCCG AATTGCCGCT TTTTCTCACA 
GTGCCACTGG TCTTCACGGC GATTGCCTAT CCGATGATCG GACTGCGGGC CGGAGTGCTG 
CACTTCTTCA ACTGCCTGGC GCTGGTCACT CTGGTGGCCA ATGTGTCAAC GTCCTTCGGA 
TATCTAATAT CCTGCGCCAG CTCCTCGACC TCGATGGCGC TGTCTGTGGG TCCGCCGGTT 
ATCATACCAT TCCTGCTCTT TGGCGGCTTC TTCTTGAACT CGGGCTCGGT GCCAGTATAC 
CTCAAATGGT TGTCGTACCT CTCATGGTTC CGTTACGCCA ACGAGGGTCT GCTGATTAAC 
CAATGGGCGG ACGTGGAGCC GGGCGAAATT AGCTGCACAT CGTCGAACAC CACGTGCCCC 
AGTTCGGGCA AGGTCATCCT GGAGACGCTT AACTTCTCCG CCGCCGATCT GCCGCTGGAC 
TACGTGGGTC TGGCCATTCT CATCGTGAGC TTCCGGGTGC TCGCATATCT GGCTCTAAGA 
CTTCGGGCCC GACGCAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476787.1
CDS	159..2222
Translation	MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNYGTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCPTNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE

Target ORF information:

RefSeq Version	NM_057439.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster white (w), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057439.2

ORF Insert Sequence:

ATGGGCCAAG AGGATCAGGA GCTATTAATT CGCGGAGGCA GCAAACACCC ATCTGCCGAG 
CATCTGAACA ATGGTGACAG CGGAGCGGCT TCGCAGAGCT GCATTAACCA GGGCTTCGGG 
CAGGCCAAAA ACTACGGCAC GCTCCGGCCA CCCAGTCCGC CGGAGGACTC CGGTTCAGGG 
AGCGGCCAAC TAGCCGAGAA CCTCACCTAT GCCTGGCACA ATATGGACAT CTTTGGGGCG 
GTCAATCAGC CGGGCTCCGG ATGGCGGCAG CTGGTCAACC GGACACGCGG ACTATTCTGC 
AACGAGCGAC ACATACCGGC GCCCAGGAAA CATTTGCTCA AGAACGTTTG CGGCGTGGCC 
TATCCGGGCG AACTTTTGGC CGTGATGGGC AGTTCCGGTG CCGGAAAGAC GACCCTGCTG 
AATGCCCTTG CCTTTCGATC GCCGCAGGGC ATCCAAGTAT CGCCATCCGG GATGCGACTG 
CTCAATGGCC AACCTGTGGA CGCCAAGGAG ATGCAGGCCA GGTGCGCCTA TGTCCAGCAG 
GATGACCTCT TTATCGGCTC CCTAACGGCC AGGGAACACC TGATTTTCCA AGCCATGGTG 
CGGATGCCAC GACATCTGAC CTATCGGCAG CGAGTGGCCC GCGTGGATCA GGTGATCCAG 
GAGCTTTCGC TCAGCAAATG TCAGCACACG ATCATCGGTG TGCCCGGCAG GGTGAAAGGT 
CTGTCCGGCG GAGAAAGGAA GCGTCTGGCA TTCGCCTCCG AGGCTCTAAC CGATCCGCCG 
CTTCTGATCT GCGATGAGCC CACCTCCGGA CTGGACTCCT TTACCGCCCA CAGCGTCGTC 
CAGGTGCTGA AGAAGCTGTC GCAGAAGGGC AAGACCGTCA TCCTGACCAT TCATCAGCCG 
TCTTCCGAGC TGTTTGAGCT CTTTGACAAG ATCCTTCTGA TGGCCGAGGG CAGGGTAGCT 
TTCTTGGGCA CTCCCAGCGA AGCCGTCGAC TTCTTTTCCT ACGTGGGTGC CCAGTGTCCT 
ACCAACTACA ATCCGGCGGA CTTTTACGTA CAGGTGTTGG CCGTTGTGCC CGGACGGGAG 
ATCGAGTCCC GTGATCGGAT CGCCAAGATA TGCGACAATT TTGCCATTAG CAAAGTAGCC 
CGGGATATGG AGCAGTTGTT GGCCACCAAA AATCTGGAGA AGCCACTGGA GCAGCCGGAG 
AATGGGTACA CCTACAAGGC CACCTGGTTC ATGCAGTTCC GGGCGGTCCT GTGGCGATCC 
TGGCTGTCGG TGCTCAAGGA ACCACTCCTC GTAAAAGTGC GACTTATTCA GACAACGATG 
GTTGCCATCT TGATTGGCCT CATCTTTTTG GGCCAACAAC TCACGCAAGT GGGTGTGATG 
AATATCAACG GAGCCATCTT CCTCTTCCTG ACCAACATGA CCTTTCAAAA CGTCTTTGCC 
ACGATAAATG TGTTCACCTC AGAGCTGCCA GTTTTTATGA GGGAGGCCCG AAGTCGACTT 
TATCGCTGTG ACACATACTT TCTGGGCAAA ACGATTGCCG AATTGCCGCT TTTTCTCACA 
GTGCCACTGG TCTTCACGGC GATTGCCTAT CCGATGATCG GACTGCGGGC CGGAGTGCTG 
CACTTCTTCA ACTGCCTGGC GCTGGTCACT CTGGTGGCCA ATGTGTCAAC GTCCTTCGGA 
TATCTAATAT CCTGCGCCAG CTCCTCGACC TCGATGGCGC TGTCTGTGGG TCCGCCGGTT 
ATCATACCAT TCCTGCTCTT TGGCGGCTTC TTCTTGAACT CGGGCTCGGT GCCAGTATAC 
CTCAAATGGT TGTCGTACCT CTCATGGTTC CGTTACGCCA ACGAGGGTCT GCTGATTAAC 
CAATGGGCGG ACGTGGAGCC GGGCGAAATT AGCTGCACAT CGTCGAACAC CACGTGCCCC 
AGTTCGGGCA AGGTCATCCT GGAGACGCTT AACTTCTCCG CCGCCGATCT GCCGCTGGAC 
TACGTGGGTC TGGCCATTCT CATCGTGAGC TTCCGGGTGC TCGCATATCT GGCTCTAAGA 
CTTCGGGCCC GACGCAAGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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w cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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