PsGEF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	PsGEF
Entrez Gene ID	31224
Full Name	Protostome-specific GEF
Synonyms	CG14045,CG14047,CG43947,DmPsGEF,Dmel\CG43947,Dmel_CG14045,Dmel_CG14047,EG:BACH48C10.4,EG:BACH7M4.1,EG:BACH7M4.2,FBgn0264598,PsGef,null
General protein information	Preferred Names Protostome-specific GEF Names CG43947 gene product from transcript CG43947-RF CG43947-PA CG43947-PB CG43947-PC CG43947-PE CG43947-PF CG43947-PG CG43947-PH CG43947-PI PsGEF-PA PsGEF-PB PsGEF-PC PsGEF-PE PsGEF-PF PsGEF-PG PsGEF-PH PsGEF-PI protostome-specific GEF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A2-3A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_130645.6	NP_570001.5	Protostome-specific GEF, isoform F
NM_001272258.1	NP_001259187.1	Protostome-specific GEF, isoform G
NM_206610.5	NP_996333.4	Protostome-specific GEF, isoform E
NM_001272259.1	NP_001259188.1	Protostome-specific GEF, isoform H
NM_001169176.2	NP_001162647.1	Protostome-specific GEF, isoform C
NM_130646.3	NP_570002.1	Protostome-specific GEF, isoform A
NM_001272260.1	NP_001259189.1	Protostome-specific GEF, isoform I
NM_001169175.2	NP_001162646.1	Protostome-specific GEF, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	PsGEF	NP_001162647.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004701	XP_554654.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PsGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PsGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03001	NM_001169175.2 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00601	NM_130646.3 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa02999	NM_001272260.1 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa03000	NM_001169176.2 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa02997	NM_001272259.1 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02998	NM_001272258.1 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa00599	NM_130645.6 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa00600	NM_206610.5 Latest version!	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PsGEF ( NM_001169175.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162646.1 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03001
Clone ID Related Accession (Same CDS sequence)	NM_001169175.2
Accession Version	NM_001169175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169175.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGGTA 
GGCAAAGGTG TCTTCGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162646.1
CDS	260..2620
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKEVGKGVFV

Target ORF information:

RefSeq Version	NM_001169175.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169175.2

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGGTA 
GGCAAAGGTG TCTTCGTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PsGEF ( NM_130646.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_570002.1 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00601
Clone ID Related Accession (Same CDS sequence)	NM_130646.3
Accession Version	NM_130646.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130646.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA ACTCAGTTCC CGTTACTCAG TCCGAGTGGC AAGCTCGCGT GAAAAGCGGC 
ACAGAACGGC GAACGGCAGA ACGGCTGGCG AAGCTCGAAA ACGCAGGAAA CGAGGCAAAA 
CGCAGCGAGT TCCTGGGCTG CAGCACTTTT CCACTGAATG AGTTAGTGCA TCCGGATTCG 
GGCGTCTCGG CAGGATCCTA CAAGCTGCAT GCACAGGCAT GTCCTCCGCC TACTAGCCGC 
CATAGCCAAC CAAAAGCCAA CGCAGGCCAG GATCAAAAGC AGGAAACGGA AGCGGCGAAG 
GATCAGGATA AACAGCAGGA CGATTCCCCC GCAGAAATGG CAGCAGCCGT CACAGTCACG 
CCCCAAAAAC CGGTTGCCAC TGGATCCGGT TCGGGATCGA ATTCCGCAGC GATGGCACTG 
AACGACGAGG TGATCAGCAT TAGCATCGAT AGCATGGGCA AAGAGGATCC TCTGCTACCC 
CAGCAGCCCC AGCAGCCCAT GAAGCTCAGC AAGAAGGCAC TCCACCAGCG AGATGCCGAC 
GAGAATCTCT TTTTAAGATT CCTGGAGCTG GATCCGCCAG CGGATGGCAA TGCAAACAGT 
ACCACCACCC AGGCACAAGC GACGGGCTCC CAGTCCTCGG CGTCGAAGGC CAACGAAAGC 
AATGCCAACC ACTTGAACAA TGGGACATCC GGTGGACGCA GACAGTCCAC CATGCCAAAT 
AGCGGAGGAT CTAGCGTAGG AGGCGCTGTC CGCCAGCAGC AGGGACGTAC CCCATTCACC 
ATGACGAAAA GACTTACGCG AACCGAGGAA CGGGGCTTTG GATTCTCTAT TGTCTGGACG 
CATCCGCCAC GGGTGGAGAA AATCGAGGCG GGACTCTCGG CCGATCGGTG TGGCATTTTG 
CCCGGCGACT ATGTGATCTT TGTGGACAAG CATAACGTCG TCACGATGCC CGAGGCTGAT 
GTTCTGAATT TGATACGATC GCAGGGCTCG TCCTTGACGT TGGAGATATT CAGAAGGTCA 
GGCGCGGGCG CCACAACAAT CACGTCGACG GACTTGGGCC AGAACAATGT ACACATAAGC 
ACCAGATTGG GCGCGGCAGT GGGCTTGGGC AGCGAGGAGC ACACCCTGGC CACCACCGCA 
ACAGGAACGA CCACCGTAAT GAGTCTGCAA AGGACCACCA GTACGAGGAT CCAGCCATTG 
GCAAATAGCA TGAGCCGGCC GGCTACCGCA TGCTCGGGAA CCACCTCCTC CATCGAGGCG 
GCCAAAAGGA GGCTTCACCT GCCACAGGTC ACCTTCAGCA AGGAGTCGAT TGTGCCTGTC 
ACGGACAACC GTAGGCGCTT CCTGCTCCAG CTGATTAGTC GGGAGCAGAA CTTCACGGCT 
GCCCTGCACT TCGGAGTGGA CCGCTTTGTG CAGCCGCTTG GCGAACGAAA GGATCTGATC 
TCGCCGAACG ATCATCGCAC TTTGTTCCAG AACATCGACG AGCTGCTCAG GATTGCCGAG 
GACATCCTGG AGCAGTTGTG CAGCAGCGAT CAGCAGGACC AGGAGCCACA AATGAACTTT 
GCCTCGCGGG TCTACCTATC AAAAACTACA GCGATCTGTG CGGCATACAA GAAGTACTGC 
AATGGAATTA AGCGGGCCGA CTGTGTTTTG GTCAATAAGT CCCGACAGAC TGGCTCCGAG 
TTTATAGCCT TCATTACGGA ACCGGCCGTT CCACGTAAAA GACCCGATCT CACCATGTTC 
ATCCATCGAC CACTGCAGCA TTTTCGTGAA ATCCTTAAAC TGATGCAGTT GCTCGCTGGA 
AATTGCCATG TGGATACGGA GGAGCACAAG AACTTTAGCA CGGTTATCAA CGAACTGCAG 
GCTGCCTATC GGGAGATCAC TGTGAGCAGT GGTCTGATGG AACCACTGGG AGAAGGTCGC 
CCCCTGCTCA CCCTCCAGGA TCTGGAGTCC CGGATGGTCT TTACCAAATG CAAGCCTTTT 
ACGTTGGCCG TTCAGGGGCG CCAGTGGATC TTTGGCGGCG ATTTGTCCCG GGTCGAAGGT 
CGCTCCGTTA AGCCCTATTG GACACTACTA TTCAGTGATA TCATCGTTTT TGCCAAGGTC 
AGCCGTGATC GAGTCCTGTT TATCACAGAG GAGCCCATTC CCATCGCCAA TGTGGTTGAC 
TCTTGTTTCC ATATGCGCAA GAAAACCACC GAGTTTCGTC TCACAGTGGA TCCCAATGGA 
CGCCTGGCGG AGAGTCCGAC GGGTTACTGT GCTCCCGATC TCACCCGCAC TCCAAAGCGA 
GGAGCCCGCC GCAAGAGCCT TATTTTAAGG GCACCTTCGC TGGAACTTAA GGCCGTTTGG 
CAGAATCTTC TCCAGCGGCA GATCTTTCTA GTTAACGCCG CCCTTGGCTC CACGCCCTTG 
TCCAGTCCCT TGGACTCGCC GGATGTTCTC AACACCCTGG TGCCGCTGAG TGATATTGGT 
CTGACCACCG CCTCCATGGG GTCGATGAAG TTACCATCGC TGGACAGCAT CCACCTGAAG 
CAGCAGCAGA AACAGCAGGT GCGTCTCTTT CGCAGCAAGC GTTTGGACAG CGGTGATTCC 
TACGAGAATC TTCAGCGTCT GGCCAGTGCG GTTCGACACC ACTGTGCCAG CACCACCACG 
AGCAGCGCCA CCCAGACGCA GTCCCATCCG CAGCCACCTC TCTGCACCAC CTCGCTGCCC 
TCGCGCACCA ACTCGCCCGC CCGGAACCAA CGCCATCCGA ATGGCCAAGG ACATGTGGGT 
GGGTCGGTGC CGAACGGAGT GGGTGGTGTG AGTTTCGGTG GCTGCAATAC AAACAGCTCT 
TGCTTGAGCA GCAGCGCTAG CTTGACTCAA ACCCAGACGC AGGTCCACAC CCAGACTCTA 
AACCATTCAC AGACCCACTC GCAGACCCAA ACCTCTGTGC TGTCCCTCTC CAGTTGCAAG 
TGCTTAACCG TCATTCCGGA GGTTACTAGT GAACCGCCAG TCAATCAACA TGCCTCGCAG 
AAACTCTTGG AGTTTAGCTT GAGCTCAGTG GGTCGATCGT TCATCGATGA GTCTGGCGGT 
GTGGTTGGTG GTCAGGTCTC CCTGACTACG CCAGAGACAC CCACACCCAA CATTTCACCC 
ACCACTTCGC AGCCCTGCAA CTCGGACGCC TTTTCCAATG ACTTCGTGGC AACATCCACG 
ACCGGGAAAG GACCGAATAT ACCGCTAGCT GAATTTGGTG GCTCCTGGGA TCTATTGGAG 
CTCGATCTCC AGCTGCACGA GGTCAATTTA GATCCCAGCT ACGACACGGA TGTGGAGGAG 
TGCATCTTTT TGGGCGACGA GGAGGACAGA GAGGGCACTG AGGGACACGG ACACGACGAG 
GACAGTGACG AGGACGATAG CGTGGTAGTG GACGATCCCT TCGGCATGCT ACCAACCAGA 
CCAACGCCAC CGGACTCGCT GGACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570002.1
CDS	260..4849
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLNSVPVTQSEWQARVKSGTERRTAERLAKLENAGNEAKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFTAALHFGVDRFVQPLGERKDLISPNDHRTLFQNIDELLRIAEDILEQLCSSDQQDQEPQMNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTLLFSDIIVFAKVSRDRVLFITEEPIPIANVVDSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAPDLTRTPKRGARRKSLILRAPSLELKAVWQNLLQRQIFLVNAALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQVRLFRSKRLDSGDSYENLQRLASAVRHHCASTTTSSATQTQSHPQPPLCTTSLPSRTNSPARNQRHPNGQGHVGGSVPNGVGGVSFGGCNTNSSCLSSSASLTQTQTQVHTQTLNHSQTHSQTQTSVLSLSSCKCLTVIPEVTSEPPVNQHASQKLLEFSLSSVGRSFIDESGGVVGGQVSLTTPETPTPNISPTTSQPCNSDAFSNDFVATSTTGKGPNIPLAEFGGSWDLLELDLQLHEVNLDPSYDTDVEECIFLGDEEDREGTEGHGHDEDSDEDDSVVVDDPFGMLPTRPTPPDSLDL

Target ORF information:

RefSeq Version	NM_130646.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130646.3

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA ACTCAGTTCC CGTTACTCAG TCCGAGTGGC AAGCTCGCGT GAAAAGCGGC 
ACAGAACGGC GAACGGCAGA ACGGCTGGCG AAGCTCGAAA ACGCAGGAAA CGAGGCAAAA 
CGCAGCGAGT TCCTGGGCTG CAGCACTTTT CCACTGAATG AGTTAGTGCA TCCGGATTCG 
GGCGTCTCGG CAGGATCCTA CAAGCTGCAT GCACAGGCAT GTCCTCCGCC TACTAGCCGC 
CATAGCCAAC CAAAAGCCAA CGCAGGCCAG GATCAAAAGC AGGAAACGGA AGCGGCGAAG 
GATCAGGATA AACAGCAGGA CGATTCCCCC GCAGAAATGG CAGCAGCCGT CACAGTCACG 
CCCCAAAAAC CGGTTGCCAC TGGATCCGGT TCGGGATCGA ATTCCGCAGC GATGGCACTG 
AACGACGAGG TGATCAGCAT TAGCATCGAT AGCATGGGCA AAGAGGATCC TCTGCTACCC 
CAGCAGCCCC AGCAGCCCAT GAAGCTCAGC AAGAAGGCAC TCCACCAGCG AGATGCCGAC 
GAGAATCTCT TTTTAAGATT CCTGGAGCTG GATCCGCCAG CGGATGGCAA TGCAAACAGT 
ACCACCACCC AGGCACAAGC GACGGGCTCC CAGTCCTCGG CGTCGAAGGC CAACGAAAGC 
AATGCCAACC ACTTGAACAA TGGGACATCC GGTGGACGCA GACAGTCCAC CATGCCAAAT 
AGCGGAGGAT CTAGCGTAGG AGGCGCTGTC CGCCAGCAGC AGGGACGTAC CCCATTCACC 
ATGACGAAAA GACTTACGCG AACCGAGGAA CGGGGCTTTG GATTCTCTAT TGTCTGGACG 
CATCCGCCAC GGGTGGAGAA AATCGAGGCG GGACTCTCGG CCGATCGGTG TGGCATTTTG 
CCCGGCGACT ATGTGATCTT TGTGGACAAG CATAACGTCG TCACGATGCC CGAGGCTGAT 
GTTCTGAATT TGATACGATC GCAGGGCTCG TCCTTGACGT TGGAGATATT CAGAAGGTCA 
GGCGCGGGCG CCACAACAAT CACGTCGACG GACTTGGGCC AGAACAATGT ACACATAAGC 
ACCAGATTGG GCGCGGCAGT GGGCTTGGGC AGCGAGGAGC ACACCCTGGC CACCACCGCA 
ACAGGAACGA CCACCGTAAT GAGTCTGCAA AGGACCACCA GTACGAGGAT CCAGCCATTG 
GCAAATAGCA TGAGCCGGCC GGCTACCGCA TGCTCGGGAA CCACCTCCTC CATCGAGGCG 
GCCAAAAGGA GGCTTCACCT GCCACAGGTC ACCTTCAGCA AGGAGTCGAT TGTGCCTGTC 
ACGGACAACC GTAGGCGCTT CCTGCTCCAG CTGATTAGTC GGGAGCAGAA CTTCACGGCT 
GCCCTGCACT TCGGAGTGGA CCGCTTTGTG CAGCCGCTTG GCGAACGAAA GGATCTGATC 
TCGCCGAACG ATCATCGCAC TTTGTTCCAG AACATCGACG AGCTGCTCAG GATTGCCGAG 
GACATCCTGG AGCAGTTGTG CAGCAGCGAT CAGCAGGACC AGGAGCCACA AATGAACTTT 
GCCTCGCGGG TCTACCTATC AAAAACTACA GCGATCTGTG CGGCATACAA GAAGTACTGC 
AATGGAATTA AGCGGGCCGA CTGTGTTTTG GTCAATAAGT CCCGACAGAC TGGCTCCGAG 
TTTATAGCCT TCATTACGGA ACCGGCCGTT CCACGTAAAA GACCCGATCT CACCATGTTC 
ATCCATCGAC CACTGCAGCA TTTTCGTGAA ATCCTTAAAC TGATGCAGTT GCTCGCTGGA 
AATTGCCATG TGGATACGGA GGAGCACAAG AACTTTAGCA CGGTTATCAA CGAACTGCAG 
GCTGCCTATC GGGAGATCAC TGTGAGCAGT GGTCTGATGG AACCACTGGG AGAAGGTCGC 
CCCCTGCTCA CCCTCCAGGA TCTGGAGTCC CGGATGGTCT TTACCAAATG CAAGCCTTTT 
ACGTTGGCCG TTCAGGGGCG CCAGTGGATC TTTGGCGGCG ATTTGTCCCG GGTCGAAGGT 
CGCTCCGTTA AGCCCTATTG GACACTACTA TTCAGTGATA TCATCGTTTT TGCCAAGGTC 
AGCCGTGATC GAGTCCTGTT TATCACAGAG GAGCCCATTC CCATCGCCAA TGTGGTTGAC 
TCTTGTTTCC ATATGCGCAA GAAAACCACC GAGTTTCGTC TCACAGTGGA TCCCAATGGA 
CGCCTGGCGG AGAGTCCGAC GGGTTACTGT GCTCCCGATC TCACCCGCAC TCCAAAGCGA 
GGAGCCCGCC GCAAGAGCCT TATTTTAAGG GCACCTTCGC TGGAACTTAA GGCCGTTTGG 
CAGAATCTTC TCCAGCGGCA GATCTTTCTA GTTAACGCCG CCCTTGGCTC CACGCCCTTG 
TCCAGTCCCT TGGACTCGCC GGATGTTCTC AACACCCTGG TGCCGCTGAG TGATATTGGT 
CTGACCACCG CCTCCATGGG GTCGATGAAG TTACCATCGC TGGACAGCAT CCACCTGAAG 
CAGCAGCAGA AACAGCAGGT GCGTCTCTTT CGCAGCAAGC GTTTGGACAG CGGTGATTCC 
TACGAGAATC TTCAGCGTCT GGCCAGTGCG GTTCGACACC ACTGTGCCAG CACCACCACG 
AGCAGCGCCA CCCAGACGCA GTCCCATCCG CAGCCACCTC TCTGCACCAC CTCGCTGCCC 
TCGCGCACCA ACTCGCCCGC CCGGAACCAA CGCCATCCGA ATGGCCAAGG ACATGTGGGT 
GGGTCGGTGC CGAACGGAGT GGGTGGTGTG AGTTTCGGTG GCTGCAATAC AAACAGCTCT 
TGCTTGAGCA GCAGCGCTAG CTTGACTCAA ACCCAGACGC AGGTCCACAC CCAGACTCTA 
AACCATTCAC AGACCCACTC GCAGACCCAA ACCTCTGTGC TGTCCCTCTC CAGTTGCAAG 
TGCTTAACCG TCATTCCGGA GGTTACTAGT GAACCGCCAG TCAATCAACA TGCCTCGCAG 
AAACTCTTGG AGTTTAGCTT GAGCTCAGTG GGTCGATCGT TCATCGATGA GTCTGGCGGT 
GTGGTTGGTG GTCAGGTCTC CCTGACTACG CCAGAGACAC CCACACCCAA CATTTCACCC 
ACCACTTCGC AGCCCTGCAA CTCGGACGCC TTTTCCAATG ACTTCGTGGC AACATCCACG 
ACCGGGAAAG GACCGAATAT ACCGCTAGCT GAATTTGGTG GCTCCTGGGA TCTATTGGAG 
CTCGATCTCC AGCTGCACGA GGTCAATTTA GATCCCAGCT ACGACACGGA TGTGGAGGAG 
TGCATCTTTT TGGGCGACGA GGAGGACAGA GAGGGCACTG AGGGACACGG ACACGACGAG 
GACAGTGACG AGGACGATAG CGTGGTAGTG GACGATCCCT TCGGCATGCT ACCAACCAGA 
CCAACGCCAC CGGACTCGCT GGACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PsGEF ( NM_001272260.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259189.1 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant I, mRNA.

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CloneID	OFa02999
Clone ID Related Accession (Same CDS sequence)	NM_001272260.1
Accession Version	NM_001272260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGAGGC CACCGATGAC AGCGTCGGAG 
CGATGCCCAA TAGCAGTAGA AGTGCCGGAG AAGGAAACCC AGTTATCACC GGCTCCAGAC 
TACGTCATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259189.1
CDS	260..4471
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFTAALHFGVDRFVQPLGERKDLISPNDHRTLFQNIDELLRIAEDILEQLCSSDQQDQEPQMNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTLLFSDIIVFAKVSRDRVLFITEEPIPIANVVDSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAPDLTRTPKRGARRKSLILRAPSLELKAVWQNLLQRQIFLVNAALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQRTNNSHGHVHSATGVATDRTADRSHGHNHGIHGGHVEQIELLIDEKCRILNKTGTPKSSALHLANWMKGQLDKQQQQARLAAIARSQENVSAEDEQLIFNSDSEQDERITYWTRQQLEKRTKELNLAKENGGLSARPNFGGKRLSGVEELSMSATSDIYSTSEAEGVTSVISQSHSTTSDSQRPPMTASERCPIAVEVPEKETQLSPAPDYVI

Target ORF information:

RefSeq Version	NM_001272260.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272260.1

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGAGGC CACCGATGAC AGCGTCGGAG 
CGATGCCCAA TAGCAGTAGA AGTGCCGGAG AAGGAAACCC AGTTATCACC GGCTCCAGAC 
TACGTCATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PsGEF ( NM_001169176.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162647.1 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant C, mRNA.

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CloneID	OFa03000
Clone ID Related Accession (Same CDS sequence)	NM_001169176.2
Accession Version	NM_001169176.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001169176.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG GTGCGTCTCT TTCGCAGCAA GCGTTTGGAC 
AGCGGTGATT CCTACGAGAA TCTTCAGCGT CTGGCCAGTG CGGTTCGACA CCACTGTGCC 
AGCACCACCA CGAGCAGCGC CACCCAGACG CAGTCCCATC CGCAGCCACC TCTCTGCACC 
ACCTCGCTGC CCTCGCGCAC CAACTCGCCC GCCCGGAACC AACGCCATCC GAATGGCCAA 
GGACATGTGG GTGGGTCGGT GCCGAACGGA GTGGGTGGTG TGAGTTTCGG TGGCTGCAAT 
ACAAACAGCT CTTGCTTGAG CAGCAGCGCT AGCTTGACTC AAACCCAGAC GCAGGTCCAC 
ACCCAGACTC TAAACCATTC ACAGACCCAC TCGCAGACCC AAACCTCTGT GCTGTCCCTC 
TCCAGTTGCA AGTGCTTAAC CGTCATTCCG GAGGTTACTA GTGAACCGCC AGTCAATCAA 
CATGCCTCGC AGAAACTCTT GGAGTTTAGC TTGAGCTCAG TGGGTCGATC GTTCATCGAT 
GAGTCTGGCG GTGTGGTTGG TGGTCAGGTC TCCCTGACTA CGCCAGAGAC ACCCACACCC 
AACATTTCAC CCACCACTTC GCAGCCCTGC AACTCGGACG CCTTTTCCAA TGACTTCGTG 
GCAACATCCA CGACCGGGAA AGGACCGAAT ATACCGCTAG CTGAATTTGG TGGCTCCTGG 
GATCTATTGG AGCTCGATCT CCAGCTGCAC GAGGTCAATT TAGATCCCAG CTACGACACG 
GATGTGGAGG AGTGCATCTT TTTGGGCGAC GAGGAGGACA GAGAGGGCAC TGAGGGACAC 
GGACACGACG AGGACAGTGA CGAGGACGAT AGCGTGGTAG TGGACGATCC CTTCGGCATG 
CTACCAACCA GACCAACGCC ACCGGACTCG CTGGACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162647.1
CDS	260..4741
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFTAALHFGVDRFVQPLGERKDLISPNDHRTLFQNIDELLRIAEDILEQLCSSDQQDQEPQMNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTLLFSDIIVFAKVSRDRVLFITEEPIPIANVVDSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAPDLTRTPKRGARRKSLILRAPSLELKAVWQNLLQRQIFLVNAALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQVRLFRSKRLDSGDSYENLQRLASAVRHHCASTTTSSATQTQSHPQPPLCTTSLPSRTNSPARNQRHPNGQGHVGGSVPNGVGGVSFGGCNTNSSCLSSSASLTQTQTQVHTQTLNHSQTHSQTQTSVLSLSSCKCLTVIPEVTSEPPVNQHASQKLLEFSLSSVGRSFIDESGGVVGGQVSLTTPETPTPNISPTTSQPCNSDAFSNDFVATSTTGKGPNIPLAEFGGSWDLLELDLQLHEVNLDPSYDTDVEECIFLGDEEDREGTEGHGHDEDSDEDDSVVVDDPFGMLPTRPTPPDSLDL

Target ORF information:

RefSeq Version	NM_001169176.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169176.2

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG GTGCGTCTCT TTCGCAGCAA GCGTTTGGAC 
AGCGGTGATT CCTACGAGAA TCTTCAGCGT CTGGCCAGTG CGGTTCGACA CCACTGTGCC 
AGCACCACCA CGAGCAGCGC CACCCAGACG CAGTCCCATC CGCAGCCACC TCTCTGCACC 
ACCTCGCTGC CCTCGCGCAC CAACTCGCCC GCCCGGAACC AACGCCATCC GAATGGCCAA 
GGACATGTGG GTGGGTCGGT GCCGAACGGA GTGGGTGGTG TGAGTTTCGG TGGCTGCAAT 
ACAAACAGCT CTTGCTTGAG CAGCAGCGCT AGCTTGACTC AAACCCAGAC GCAGGTCCAC 
ACCCAGACTC TAAACCATTC ACAGACCCAC TCGCAGACCC AAACCTCTGT GCTGTCCCTC 
TCCAGTTGCA AGTGCTTAAC CGTCATTCCG GAGGTTACTA GTGAACCGCC AGTCAATCAA 
CATGCCTCGC AGAAACTCTT GGAGTTTAGC TTGAGCTCAG TGGGTCGATC GTTCATCGAT 
GAGTCTGGCG GTGTGGTTGG TGGTCAGGTC TCCCTGACTA CGCCAGAGAC ACCCACACCC 
AACATTTCAC CCACCACTTC GCAGCCCTGC AACTCGGACG CCTTTTCCAA TGACTTCGTG 
GCAACATCCA CGACCGGGAA AGGACCGAAT ATACCGCTAG CTGAATTTGG TGGCTCCTGG 
GATCTATTGG AGCTCGATCT CCAGCTGCAC GAGGTCAATT TAGATCCCAG CTACGACACG 
GATGTGGAGG AGTGCATCTT TTTGGGCGAC GAGGAGGACA GAGAGGGCAC TGAGGGACAC 
GGACACGACG AGGACAGTGA CGAGGACGAT AGCGTGGTAG TGGACGATCC CTTCGGCATG 
CTACCAACCA GACCAACGCC ACCGGACTCG CTGGACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PsGEF ( NM_001272259.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259188.1 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa02997
Clone ID Related Accession (Same CDS sequence)	NM_001272259.1
Accession Version	NM_001272259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGATCA CCGTACGCTC TAGTCCCATT 
GTGCTGGACA AGCTGGCCGT TTGCCGTCAT TGCCACAAGA ATTGCCAGCA GAGCGGACCG 
GGTGGAGCGC GTCCTGGCGG CGGAGGCGGA GTCAACAACT CCAGTTCCAC GCCCGTCCTG 
CTCTGCAATT CGTTGAAAGT GCAGCACAGC CAATCGTCGC CAAATCGCTG CTGCAAGCTT 
AGCAATGGTA TTGCGGGAGC CACGGAAATG GCTAACCCGA AAAACAGCAA TCGAACTGGA 
ACTGGGACAG GAAGCGTGAC CAGCATGTCC AGCAGCACTA TTACGGGGGA ACTGTCCTCC 
AAAGTGGTGG CCAGTAGCAG TCGTCGGGAA ACCGGCGACA CCGAGAGCGA CGTGGCCCAA 
CTGATAACCG ATGAAATTTC CCAATCGCAA TCAGAGGCCA CCGATGACAG CGTCGGAGCG 
ATGCCCAATA GCAGTAGAAG TGCCGGAGAA GGAAACCCAG TTATCACCGG CTCCAGACTA 
CGTCATCTAG ATCCGCCAGT CTCAGTTTCA TCCTTGCCCA ATGGCCACTG CTCCGCTGGC 
GTCGGTGGTG GTTCGCCAAA ACCGCTGCCA CCACCACGTC GCACCCGCGT TGTGGCCCAA 
ATGTGCCAGG AGCCACCAAG GCCAAAGGCC AACCAGCAGG TGAAGGCCAT TGTGACCATT 
ACGGAGACGG CCAGGATAAT GCGGAGCAGT CCAACGAAAG GCTCGGGTTC GGGATCGGCA 
ACGGGCACGG GCTCGGTGGG TGGTTCCCCA GCCAAGTATG CCGCCTGCCA CTGTCGCTGT 
ACTCCAGAGG ACTTTACCCA CGCTCAACTC TCGCCGGACA AAATGGAGCA CCAGACCTGC 
AAGCTACTGG AATCACCCAA GCAAACGACC ACCACACTGG AGCAGAAACA GGAAGACGAG 
CAGTTGTCCC TGATGCTCAT TGGTCTGGCG CAGCTTGCTC CAGCTGCCAC ACTTTGTGGG 
CAGGAAAGGA TCCCCAAGGA GGAGAATAGT ACACCCACCA TAGCCGTGGT GCCGCCCACT 
CCCGATTCGG TGCTCACTAA AACCAGCACT CACGTTTGGG ACAACAGTGG CTGCTCGTCA 
AACGGCGGAG GAACCTCAAC CACCAGCACC GCTACCACTA CAACGAAACA ACCAAGGCAG 
GCCATTATTG AGAATATTCC AGAGGACTCG TGTGACGAGT CCCCATTGGA TGAGGAACCA 
CCCTATCGAC CCATGAGCAG TGCTCTTCGC CGATTCGGCA CCATGTCAAG CTTAGAGAAA 
CTACCTTCCG ATGATCGAAT GGATGAGGCC GATGAGCTAG ATGATGATTT GGATTCATTT 
AAGCCAAATG GCCACGACGG CAACAGTCCC CAAAACGAGG AAGACGACGA GGATGCGGAC 
TCGGAACGAG CTTTGGTACA AAACGACCTG GGGGCCTCCA GTTCGTCCGG AGTGATTGTC 
AATGGCGAGG GCCTGGCCAG TGGTGCGTGG ACAAATCGGG CGGGAGCCTA TGTCTCCGAC 
AAGATGTCCT TCTTCGAGGA ATCGCGCGCT TTCATTGATA AGTACTTGGG TCGCTGGAAT 
GCTGGCGATG CACAACAGCA GCACCAGCAG GGAACCGCCT CGGAGACGGA TGAGCAAATG 
GACGAGTGCA CCTCGGGAGC CACCAGTGGT GAAGAGGTCT GGGGCACACC CACCAGTGGC 
GGGGATAACG ATGATCAGGA CTTGCAGCTG ATCAACTCGG AAAACACACA TTCGTCGCCA 
ACCAAGTCGA GCACCTCCTT GAATGACGAC GACGACACGG AGTTGATGAT GGACGAGCTG 
TTAATGGCGC CACCTATGAC TGCCAGCACA ATTCGGGGAT TGTTGCCACG GGGGAAGCAA 
CGACCAATGG CCACTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259188.1
CDS	260..6280
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFTAALHFGVDRFVQPLGERKDLISPNDHRTLFQNIDELLRIAEDILEQLCSSDQQDQEPQMNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTLLFSDIIVFAKVSRDRVLFITEEPIPIANVVDSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAPDLTRTPKRGARRKSLILRAPSLELKAVWQNLLQRQIFLVNAALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQRTNNSHGHVHSATGVATDRTADRSHGHNHGIHGGHVEQIELLIDEKCRILNKTGTPKSSALHLANWMKGQLDKQQQQARLAAIARSQENVSAEDEQLIFNSDSEQDERITYWTRQQLEKRTKELNLAKENGGLSARPNFGGKRLSGVEELSMSATSDIYSTSEAEGVTSVISQSHSTTSDSQITVRSSPIVLDKLAVCRHCHKNCQQSGPGGARPGGGGGVNNSSSTPVLLCNSLKVQHSQSSPNRCCKLSNGIAGATEMANPKNSNRTGTGTGSVTSMSSSTITGELSSKVVASSSRRETGDTESDVAQLITDEISQSQSEATDDSVGAMPNSSRSAGEGNPVITGSRLRHLDPPVSVSSLPNGHCSAGVGGGSPKPLPPPRRTRVVAQMCQEPPRPKANQQVKAIVTITETARIMRSSPTKGSGSGSATGTGSVGGSPAKYAACHCRCTPEDFTHAQLSPDKMEHQTCKLLESPKQTTTTLEQKQEDEQLSLMLIGLAQLAPAATLCGQERIPKEENSTPTIAVVPPTPDSVLTKTSTHVWDNSGCSSNGGGTSTTSTATTTTKQPRQAIIENIPEDSCDESPLDEEPPYRPMSSALRRFGTMSSLEKLPSDDRMDEADELDDDLDSFKPNGHDGNSPQNEEDDEDADSERALVQNDLGASSSSGVIVNGEGLASGAWTNRAGAYVSDKMSFFEESRAFIDKYLGRWNAGDAQQQHQQGTASETDEQMDECTSGATSGEEVWGTPTSGGDNDDQDLQLINSENTHSSPTKSSTSLNDDDDTELMMDELLMAPPMTASTIRGLLPRGKQRPMATT

Target ORF information:

RefSeq Version	NM_001272259.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272259.1

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGATCA CCGTACGCTC TAGTCCCATT 
GTGCTGGACA AGCTGGCCGT TTGCCGTCAT TGCCACAAGA ATTGCCAGCA GAGCGGACCG 
GGTGGAGCGC GTCCTGGCGG CGGAGGCGGA GTCAACAACT CCAGTTCCAC GCCCGTCCTG 
CTCTGCAATT CGTTGAAAGT GCAGCACAGC CAATCGTCGC CAAATCGCTG CTGCAAGCTT 
AGCAATGGTA TTGCGGGAGC CACGGAAATG GCTAACCCGA AAAACAGCAA TCGAACTGGA 
ACTGGGACAG GAAGCGTGAC CAGCATGTCC AGCAGCACTA TTACGGGGGA ACTGTCCTCC 
AAAGTGGTGG CCAGTAGCAG TCGTCGGGAA ACCGGCGACA CCGAGAGCGA CGTGGCCCAA 
CTGATAACCG ATGAAATTTC CCAATCGCAA TCAGAGGCCA CCGATGACAG CGTCGGAGCG 
ATGCCCAATA GCAGTAGAAG TGCCGGAGAA GGAAACCCAG TTATCACCGG CTCCAGACTA 
CGTCATCTAG ATCCGCCAGT CTCAGTTTCA TCCTTGCCCA ATGGCCACTG CTCCGCTGGC 
GTCGGTGGTG GTTCGCCAAA ACCGCTGCCA CCACCACGTC GCACCCGCGT TGTGGCCCAA 
ATGTGCCAGG AGCCACCAAG GCCAAAGGCC AACCAGCAGG TGAAGGCCAT TGTGACCATT 
ACGGAGACGG CCAGGATAAT GCGGAGCAGT CCAACGAAAG GCTCGGGTTC GGGATCGGCA 
ACGGGCACGG GCTCGGTGGG TGGTTCCCCA GCCAAGTATG CCGCCTGCCA CTGTCGCTGT 
ACTCCAGAGG ACTTTACCCA CGCTCAACTC TCGCCGGACA AAATGGAGCA CCAGACCTGC 
AAGCTACTGG AATCACCCAA GCAAACGACC ACCACACTGG AGCAGAAACA GGAAGACGAG 
CAGTTGTCCC TGATGCTCAT TGGTCTGGCG CAGCTTGCTC CAGCTGCCAC ACTTTGTGGG 
CAGGAAAGGA TCCCCAAGGA GGAGAATAGT ACACCCACCA TAGCCGTGGT GCCGCCCACT 
CCCGATTCGG TGCTCACTAA AACCAGCACT CACGTTTGGG ACAACAGTGG CTGCTCGTCA 
AACGGCGGAG GAACCTCAAC CACCAGCACC GCTACCACTA CAACGAAACA ACCAAGGCAG 
GCCATTATTG AGAATATTCC AGAGGACTCG TGTGACGAGT CCCCATTGGA TGAGGAACCA 
CCCTATCGAC CCATGAGCAG TGCTCTTCGC CGATTCGGCA CCATGTCAAG CTTAGAGAAA 
CTACCTTCCG ATGATCGAAT GGATGAGGCC GATGAGCTAG ATGATGATTT GGATTCATTT 
AAGCCAAATG GCCACGACGG CAACAGTCCC CAAAACGAGG AAGACGACGA GGATGCGGAC 
TCGGAACGAG CTTTGGTACA AAACGACCTG GGGGCCTCCA GTTCGTCCGG AGTGATTGTC 
AATGGCGAGG GCCTGGCCAG TGGTGCGTGG ACAAATCGGG CGGGAGCCTA TGTCTCCGAC 
AAGATGTCCT TCTTCGAGGA ATCGCGCGCT TTCATTGATA AGTACTTGGG TCGCTGGAAT 
GCTGGCGATG CACAACAGCA GCACCAGCAG GGAACCGCCT CGGAGACGGA TGAGCAAATG 
GACGAGTGCA CCTCGGGAGC CACCAGTGGT GAAGAGGTCT GGGGCACACC CACCAGTGGC 
GGGGATAACG ATGATCAGGA CTTGCAGCTG ATCAACTCGG AAAACACACA TTCGTCGCCA 
ACCAAGTCGA GCACCTCCTT GAATGACGAC GACGACACGG AGTTGATGAT GGACGAGCTG 
TTAATGGCGC CACCTATGAC TGCCAGCACA ATTCGGGGAT TGTTGCCACG GGGGAAGCAA 
CGACCAATGG CCACTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PsGEF ( NM_001272258.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259187.1 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant G, mRNA.

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CloneID	OFa02998
Clone ID Related Accession (Same CDS sequence)	NM_001272258.1
Accession Version	NM_001272258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGATCA CCGTACGCTC TAGTCCCATT 
GTGCTGGACA AGCTGGCCGT TTGCCGTCAT TGCCACAAGA ATTGCCAGCA GAGCGGACCG 
GGTGGAGCGC GTCCTGGCGG CGGAGGCGGA GTCAACAACT CCAGTTCCAC GCCCGTCCTG 
CTCTGCAATT CGTTGAAAGT GCAGCACAGC CAATCGTCGC CAAATCGCTG CTGCAAGCTT 
AGCAATGGTA TTGCGGGAGC CACGGAAATG GCTAACCCGA AAAACAGCAA TCGAACTGGA 
ACTGGGACAG GAAGCGTGAC CAGCATGTCC AGCAGCACTA TTACGGGGGA ACTGTCCTCC 
AAAGTGGTGG CCAGTAGCAG TCGTCGGGAA ACCGGCGACA CCGAGAGCGA CGTGGCCCAA 
CTGATAACCG ATGAAATTTC CCAATCGCAA TCAGAGGCCA CCGATGACAG CGTCGGAGCG 
ATGCCCAATA GCAGTAGAAG TGCCGGAGAA GGAAACCCAG TTATCACCGG CTCCAGACTA 
CGTCATCTAG ATCCGCCAGT CTCAGTTTCA TCCTTGCCCA ATGGCCACTG CTCCGCTGGC 
GTCGGTGGTG GTTCGCCAAA ACCGCTGCCA CCACCACGTC GCACCCGCGT TGTGGCCCAA 
ATGTGCCAGG AGCCACCAAG GCCAAAGGCC AACCAGCAGG TGAAGGCCAT TGTGACCATT 
ACGGAGACGG CCAGGATAAT GCGGAGCAGT CCAACGAAAG GCTCGGGTTC GGGATCGGCA 
ACGGGCACGG GCTCGGTGGG TGGTTCCCCA GCCAAGTATG CCGCCTGCCA CTGTCGCTGT 
ACTCCAGAGG ACTTTACCCA CGCTCAACTC TCGCCGGACA AAATGGAGCA CCAGACCTGC 
AAGCTACTGG AATCACCCAA GCAAACGACC ACCACACTGG AGCAGAAACA GGAAGACGAG 
CAGTTGTCCC TGATGCTCAT TGGTCTGGCG CAGCTTGCTC CAGCTGCCAC ACTTTGTGGG 
CAGGAAAGGA TCCCCAAGGA GGAGAATAGT ACACCCACCA TAGCCGTGGT GCCGCCCACT 
CCCGATTCGG TGCTCACTAA AACCAGCACT CACGTTTGGG ACAACAGTGG CTGCTCGTCA 
AACGGCGGAG GAACCTCAAC CACCAGCACC GCTACCACTA CAACGAAACA ACCAAGGCAG 
GCCATTATTG AGAATATTCC AGAGGACTCG TGTGACGAGT CCCCATTGGA TGAGGAACCA 
CCCTATCGAC CCATGAGCAG TGCTCTTCGC CGATTCGGCA CCATGTCAAG CTTAGAGAAA 
CTACCTTCCG ATGATCGAAT GGATGAGGCC GATGAGCTAG ATGATGATTT GGATTCATTT 
AAGCCAAATG GCCACGACGG CAACAGTCCC CAAAACGAGG AAGACGACGA GGATGCGGAC 
TCGGAACGAG CTTTGGTACA AAACGACCTG GGGGCCTCCA GTTCGTCCGG AGTGATTGTC 
AATGGCGAGG GCCTGGCCAG TGGTGCGTGG ACAAATCGGG CGGGAGCCTA TGTCTCCGAC 
AAGATGTCCT TCTTCGAGGA ATCGCGCGCT TTCATTGATA AGTACTTGGG TCGCTGGAAT 
GCTGGCGATG CACAACAGCA GCACCAGCAG GGAACCGCCT CGGAGACGGA TGAGCAAATG 
GACGAGTGCA CCTCGGGAGC CACCAGTGGT GAAGAGGTCT GGGGCACACC CACCAGTGGC 
GGGGATAACG ATGATCAGGA CTTGCAGCTG ATCAACTCGG AAAACACACA TTCGTCGCCA 
ACCAAGTCGA GCACCTCCTT GAATGACGAC GACGACACGG AGTTGATGAT GGACGAGCTG 
TTAATGGCGC CACCTATGAC TGCCAGCACA ATTCGGGGAT TGTTGCCACG ACGTCGCCTT 
GAGCCCCTTT TTGAGGAGGA GACGGAGAGC GACGAAGAGA AGACGCAACA GGACAGCGAT 
GACATCAAAA AGAAGGTCGT AGTAAAATTA GTGAAGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259187.1
CDS	260..6361
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFTAALHFGVDRFVQPLGERKDLISPNDHRTLFQNIDELLRIAEDILEQLCSSDQQDQEPQMNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTLLFSDIIVFAKVSRDRVLFITEEPIPIANVVDSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAPDLTRTPKRGARRKSLILRAPSLELKAVWQNLLQRQIFLVNAALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQRTNNSHGHVHSATGVATDRTADRSHGHNHGIHGGHVEQIELLIDEKCRILNKTGTPKSSALHLANWMKGQLDKQQQQARLAAIARSQENVSAEDEQLIFNSDSEQDERITYWTRQQLEKRTKELNLAKENGGLSARPNFGGKRLSGVEELSMSATSDIYSTSEAEGVTSVISQSHSTTSDSQITVRSSPIVLDKLAVCRHCHKNCQQSGPGGARPGGGGGVNNSSSTPVLLCNSLKVQHSQSSPNRCCKLSNGIAGATEMANPKNSNRTGTGTGSVTSMSSSTITGELSSKVVASSSRRETGDTESDVAQLITDEISQSQSEATDDSVGAMPNSSRSAGEGNPVITGSRLRHLDPPVSVSSLPNGHCSAGVGGGSPKPLPPPRRTRVVAQMCQEPPRPKANQQVKAIVTITETARIMRSSPTKGSGSGSATGTGSVGGSPAKYAACHCRCTPEDFTHAQLSPDKMEHQTCKLLESPKQTTTTLEQKQEDEQLSLMLIGLAQLAPAATLCGQERIPKEENSTPTIAVVPPTPDSVLTKTSTHVWDNSGCSSNGGGTSTTSTATTTTKQPRQAIIENIPEDSCDESPLDEEPPYRPMSSALRRFGTMSSLEKLPSDDRMDEADELDDDLDSFKPNGHDGNSPQNEEDDEDADSERALVQNDLGASSSSGVIVNGEGLASGAWTNRAGAYVSDKMSFFEESRAFIDKYLGRWNAGDAQQQHQQGTASETDEQMDECTSGATSGEEVWGTPTSGGDNDDQDLQLINSENTHSSPTKSSTSLNDDDDTELMMDELLMAPPMTASTIRGLLPRRRLEPLFEEETESDEEKTQQDSDDIKKKVVVKLVKL

Target ORF information:

RefSeq Version	NM_001272258.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272258.1

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGATCA CCGTACGCTC TAGTCCCATT 
GTGCTGGACA AGCTGGCCGT TTGCCGTCAT TGCCACAAGA ATTGCCAGCA GAGCGGACCG 
GGTGGAGCGC GTCCTGGCGG CGGAGGCGGA GTCAACAACT CCAGTTCCAC GCCCGTCCTG 
CTCTGCAATT CGTTGAAAGT GCAGCACAGC CAATCGTCGC CAAATCGCTG CTGCAAGCTT 
AGCAATGGTA TTGCGGGAGC CACGGAAATG GCTAACCCGA AAAACAGCAA TCGAACTGGA 
ACTGGGACAG GAAGCGTGAC CAGCATGTCC AGCAGCACTA TTACGGGGGA ACTGTCCTCC 
AAAGTGGTGG CCAGTAGCAG TCGTCGGGAA ACCGGCGACA CCGAGAGCGA CGTGGCCCAA 
CTGATAACCG ATGAAATTTC CCAATCGCAA TCAGAGGCCA CCGATGACAG CGTCGGAGCG 
ATGCCCAATA GCAGTAGAAG TGCCGGAGAA GGAAACCCAG TTATCACCGG CTCCAGACTA 
CGTCATCTAG ATCCGCCAGT CTCAGTTTCA TCCTTGCCCA ATGGCCACTG CTCCGCTGGC 
GTCGGTGGTG GTTCGCCAAA ACCGCTGCCA CCACCACGTC GCACCCGCGT TGTGGCCCAA 
ATGTGCCAGG AGCCACCAAG GCCAAAGGCC AACCAGCAGG TGAAGGCCAT TGTGACCATT 
ACGGAGACGG CCAGGATAAT GCGGAGCAGT CCAACGAAAG GCTCGGGTTC GGGATCGGCA 
ACGGGCACGG GCTCGGTGGG TGGTTCCCCA GCCAAGTATG CCGCCTGCCA CTGTCGCTGT 
ACTCCAGAGG ACTTTACCCA CGCTCAACTC TCGCCGGACA AAATGGAGCA CCAGACCTGC 
AAGCTACTGG AATCACCCAA GCAAACGACC ACCACACTGG AGCAGAAACA GGAAGACGAG 
CAGTTGTCCC TGATGCTCAT TGGTCTGGCG CAGCTTGCTC CAGCTGCCAC ACTTTGTGGG 
CAGGAAAGGA TCCCCAAGGA GGAGAATAGT ACACCCACCA TAGCCGTGGT GCCGCCCACT 
CCCGATTCGG TGCTCACTAA AACCAGCACT CACGTTTGGG ACAACAGTGG CTGCTCGTCA 
AACGGCGGAG GAACCTCAAC CACCAGCACC GCTACCACTA CAACGAAACA ACCAAGGCAG 
GCCATTATTG AGAATATTCC AGAGGACTCG TGTGACGAGT CCCCATTGGA TGAGGAACCA 
CCCTATCGAC CCATGAGCAG TGCTCTTCGC CGATTCGGCA CCATGTCAAG CTTAGAGAAA 
CTACCTTCCG ATGATCGAAT GGATGAGGCC GATGAGCTAG ATGATGATTT GGATTCATTT 
AAGCCAAATG GCCACGACGG CAACAGTCCC CAAAACGAGG AAGACGACGA GGATGCGGAC 
TCGGAACGAG CTTTGGTACA AAACGACCTG GGGGCCTCCA GTTCGTCCGG AGTGATTGTC 
AATGGCGAGG GCCTGGCCAG TGGTGCGTGG ACAAATCGGG CGGGAGCCTA TGTCTCCGAC 
AAGATGTCCT TCTTCGAGGA ATCGCGCGCT TTCATTGATA AGTACTTGGG TCGCTGGAAT 
GCTGGCGATG CACAACAGCA GCACCAGCAG GGAACCGCCT CGGAGACGGA TGAGCAAATG 
GACGAGTGCA CCTCGGGAGC CACCAGTGGT GAAGAGGTCT GGGGCACACC CACCAGTGGC 
GGGGATAACG ATGATCAGGA CTTGCAGCTG ATCAACTCGG AAAACACACA TTCGTCGCCA 
ACCAAGTCGA GCACCTCCTT GAATGACGAC GACGACACGG AGTTGATGAT GGACGAGCTG 
TTAATGGCGC CACCTATGAC TGCCAGCACA ATTCGGGGAT TGTTGCCACG ACGTCGCCTT 
GAGCCCCTTT TTGAGGAGGA GACGGAGAGC GACGAAGAGA AGACGCAACA GGACAGCGAT 
GACATCAAAA AGAAGGTCGT AGTAAAATTA GTGAAGCTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PsGEF ( NM_130645.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_570001.5 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant F, mRNA.

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CloneID	OFa00599
Clone ID Related Accession (Same CDS sequence)	NM_130645.6
Accession Version	NM_130645.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130645.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGATCA CCGTACGCTC TAGTCCCATT 
GTGCTGGACA AGCTGGCCGT TTGCCGTCAT TGCCACAAGA ATTGCCAGCA GAGCGGACCG 
GGTGGAGCGC GTCCTGGCGG CGGAGGCGGA GTCAACAACT CCAGTTCCAC GCCCGTCCTG 
CTCTGCAATT CGTTGAAAGT GCAGCACAGC CAATCGTCGC CAAATCGCTG CTGCAAGCTT 
AGCAATGGTA TTGCGGGAGC CACGGAAATG GCTAACCCGA AAAACAGCAA TCGAACTGGA 
ACTGGGACAG GAAGCGTGAC CAGCATGTCC AGCAGCACTA TTACGGGGGA ACTGTCCTCC 
AAAGTGGTGG CCAGTAGCAG TCGTCGGGAA ACCGGCGACA CCGAGAGCGA CGTGGCCCAA 
CTGATAACCG ATGAAATTTC CCAATCGCAA TCAGAGGCCA CCGATGACAG CGTCGGAGCG 
ATGCCCAATA GCAGTAGAAG TGCCGGAGAA GGAAACCCAG TTATCACCGG CTCCAGACTA 
CGTCATCTAG ATCCGCCAGT CTCAGTTTCA TCCTTGCCCA ATGGCCACTG CTCCGCTGGC 
GTCGGTGGTG GTTCGCCAAA ACCGCTGCCA CCACCACGTC GCACCCGCGT TGTGGCCCAA 
ATGTGCCAGG AGCCACCAAG GCCAAAGGCC AACCAGCAGG TGAAGGCCAT TGTGACCATT 
ACGGAGACGG CCAGGATAAT GCGGAGCAGT CCAACGAAAG GCTCGGGTTC GGGATCGGCA 
ACGGGCACGG GCTCGGTGGG TGGTTCCCCA GCCAAGTATG CCGCCTGCCA CTGTCGCTGT 
ACTCCAGAGG ACTTTACCCA CGCTCAACTC TCGCCGGACA AAATGGAGCA CCAGACCTGC 
AAGCTACTGG AATCACCCAA GCAAACGACC ACCACACTGG AGCAGAAACA GGAAGACGAG 
CAGTTGTCCC TGATGCTCAT TGGTCTGGCG CAGCTTGCTC CAGCTGCCAC ACTTTGTGGG 
CAGGAAAGGA TCCCCAAGGA GGAGAATAGT ACACCCACCA TAGCCGTGGT GCCGCCCACT 
CCCGATTCGG TGCTCACTAA AACCAGCACT CACGTTTGGG ACAACAGTGG CTGCTCGTCA 
AACGGCGGAG GAACCTCAAC CACCAGCACC GCTACCACTA CAACGAAACA ACCAAGGCAG 
GCCATTATTG AGAATATTCC AGAGGACTCG TGTGACGAGT CCCCATTGGA TGAGGAACCA 
CCCTATCGAC CCATGAGCAG TGCTCTTCGC CGATTCGGCA CCATGTCAAG CTTAGAGAAA 
CTACCTTCCG ATGATCGAAT GGATGAGGCC GATGAGCTAG ATGATGATTT GGATTCATTT 
AAGCCAAATG GCCACGACGG CAACAGTCCC CAAAACGAGG AAGACGACGA GGATGCGGAC 
TCGGAACGAG CTTTGGTACA AAACGACCTG GGGGCCTCCA GTTCGTCCGG AGTGATTGTC 
AATGGCGAGG GCCTGGCCAG TGGTGCGTGG ACAAATCGGG CGGGAGCCTA TGTCTCCGAC 
AAGATGTCCT TCTTCGAGGA ATCGCGCGCT TTCATTGATA AGTACTTGGG TCGCTGGAAT 
GCTGGCGATG CACAACAGCA GCACCAGCAG GGAACCGCCT CGGAGACGGA TGAGCAAATG 
GACGAGTGCA CCTCGGGAGC CACCAGTGGT GAAGAGGTCT GGGGCACACC CACCAGTGGC 
GGGGATAACG ATGATCAGGA CTTGCAGCTG ATCAACTCGG AAAACACACA TTCGTCGCCA 
ACCAAGTCGA GCACCTCCTT GAATGACGAC GACGACACGG AGTTGATGAT GGACGAGCTG 
TTAATGGCGC CACCTATGAC TGCCAGCACA ATTCGGGGAT TGTTGCCACG ACGTCGCCTT 
GAGCCCCTTT TTGAGGAGGA GACGGAGAGC GACGAAGAGA AGACGCAACA GGACAGCGAT 
GACATCAAAA AGGGGGAAGC AACGACCAAT GGCCACTACC TAGAGGATTC GGCTGGAAGT 
TCAAGCGACA GCGAGTCGGT GCCACCAGCC CCACCACTAG TTCCTCGGCC AGTGGTGCCG 
GATCGGGAGG AGGATCCGTC AGCGGAGGGC CAGCAGGAGA ATCTGGCGAT GGAGGACCAG 
GAGGACTTGC ACTCCAGCGT GGAGCGACTG CTTCCGAATC TGGCCACGAG TGCGATGGGG 
CCGCGTCCCT TACTCACCAC CCGTCTACTG CCCACCACGG CTATCGATCC TCAGCCGCCG 
GCAGTACTGG TGGCCTCATC CTGCGAGTAT CTGCTCGATC AGCGCCCCTC GAGCGTACCA 
GGACGCGATC TCGCCGGCGG GAGCATAGGC ACCACCACGA TGACATCCTC TCGGCCGACG 
CCTCTGTCTC CGGCGAAGGC AGCAGCGACA CCGATCGGGA TGAATGCGAC GATGACAACG 
AAGACGACGA CGACCACGAT GATGACGCCG AGTCGAGCTC CCTCATCGAC TATTTCAACA 
ATCAGCAGCG CGAGCGACAC TACCAGCTCC GGCGGCAGAG CCAGCGGCAG ACCTCCGAGA 
TTTGTACCGC CGCCACCGCC TCCGCGTCGC TTGCTCCTCA CGCAGACCGA TCTAATCGCT 
GGTCAGGCCA AAACGGAGCT ACCTCGAAAA AAATCTGGCG CTACTGCTGA CAGTGGCTGC 
TCGGTGGCTG TCGGCGACGC CGCCAGCGCT TCGGACCCAA CCGCTATCGG GTCAAGGACA 
ATGCCATCAA CACCGAGTAC CAGCAGCTTG GCGTCAGCGG TGGCCCCAAA ACGACCGTCA 
TCAACGATAA TAGAGACCTG CCTGCTGGGG GCACCCAGGC CCGCCTCAGC CACTTCATCA 
GCAACAGCCT CGAGAATGGC AAAGCCGATC CGCCAGAGTC CACCCACCCA GCACCAGTCG 
CAGCCGAGGG GTCAGAGCTG CCCGGCTGCA GTGCCAGCAC CATCGACGGC TGCGCCAGAC 
GCACCCACTG TCCCAATGCC CCACGCTGGA TGCGGCCTAA GCCCAAGGCT GGAAATGCGG 
CTGGCCCTCA ACCACGACAT TCTCGGGGAC GAGGACTTGA TCTGCTACGA GCCTGGTCCC 
GATCTAACGA CTATTCTGGG GCACGACCTC TCCACATTTC ATCGTCTGAC GGGTCGCGAT 
TTATTGAGTC GTTCAGCCAC GAACAGGGTG CAGCCAAAAG AGGCTGTCAT ATCGTACTCT 
CAACAACGCA ACTCAAAGAT GGACACGCCG ACGGTGAACC GCCGGCCCCG CCCCCTCTCA 
GCGGGCGCCT TGGGCGGCTC CACCAGCAAC AGCAATCCGA ACAACAGTCG CAGCAGCAGC 
AGCCACGGCA ACAGTCCGCT CGGCGGTGGT TTAACCCGTG CCGGCGTCGA GCCAGTGGGC 
CAACAGCAGC TGGTGGTTCA AGCAGGTGGT GGATCTGCTG GTCGAGTAGG CGGCGGGAGC 
GGAAGCGCCG GCGGCAACAG CACCGCCTGC AGCTCCAACA GAGGCGGGAG CAAATTAGGC 
GATCTGGAAA TCTTAGCGAG ACGCGAGAAG ATATACTCCA TGTCTCAATC GAGGAGTGGC 
TGTCGAGTCA GGGAGACGAC GTCCACGACC ACTCTGATGG CCATGGCTAC GGAGATGCGT 
TCGGGGATGG ATTCGGAGAA ACGCGACTCT TCCTGGCCCA AACGAGCAGC TGCAGCAGCC 
TCTCTGACGA GGACGAGGAG TTCTACTTCG ATGGACGGAG CGGGAGCCAT CAGTACCACA 
ACCACAACCA CCACAACAAT GACTGAGGAG GCCTTCCTTG AGACCGAAGT GGGCAGATCT 
AAGCGCTTGA TAAACTTTAT AAAGCGTCGC AACTCCGAGA TAACCGCCAG TAGCACCAGC 
AGCACCCCCA ACTTCTCCGA CGGCGCCATT GGCGAGCAGC CCCAACCGAG CCACAGCCAG 
AGCAGTCTTC TTCTCCAGAA GCTGCCGCCG GGTCAATCGC AGGCCCAGGA CTCAGTGGAA 
ATGGCCCAAA TGTCGCCGCG CAAGGATAAC GACAAGCCAT CGCTGAATCG CCGCCTCTGG 
AAGCAAATCA CCAAACGCAG ACGCACCAAC TCCGTGTCCC AAATAGTCGC AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570001.5
CDS	260..8476
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFTAALHFGVDRFVQPLGERKDLISPNDHRTLFQNIDELLRIAEDILEQLCSSDQQDQEPQMNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTLLFSDIIVFAKVSRDRVLFITEEPIPIANVVDSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAPDLTRTPKRGARRKSLILRAPSLELKAVWQNLLQRQIFLVNAALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQRTNNSHGHVHSATGVATDRTADRSHGHNHGIHGGHVEQIELLIDEKCRILNKTGTPKSSALHLANWMKGQLDKQQQQARLAAIARSQENVSAEDEQLIFNSDSEQDERITYWTRQQLEKRTKELNLAKENGGLSARPNFGGKRLSGVEELSMSATSDIYSTSEAEGVTSVISQSHSTTSDSQITVRSSPIVLDKLAVCRHCHKNCQQSGPGGARPGGGGGVNNSSSTPVLLCNSLKVQHSQSSPNRCCKLSNGIAGATEMANPKNSNRTGTGTGSVTSMSSSTITGELSSKVVASSSRRETGDTESDVAQLITDEISQSQSEATDDSVGAMPNSSRSAGEGNPVITGSRLRHLDPPVSVSSLPNGHCSAGVGGGSPKPLPPPRRTRVVAQMCQEPPRPKANQQVKAIVTITETARIMRSSPTKGSGSGSATGTGSVGGSPAKYAACHCRCTPEDFTHAQLSPDKMEHQTCKLLESPKQTTTTLEQKQEDEQLSLMLIGLAQLAPAATLCGQERIPKEENSTPTIAVVPPTPDSVLTKTSTHVWDNSGCSSNGGGTSTTSTATTTTKQPRQAIIENIPEDSCDESPLDEEPPYRPMSSALRRFGTMSSLEKLPSDDRMDEADELDDDLDSFKPNGHDGNSPQNEEDDEDADSERALVQNDLGASSSSGVIVNGEGLASGAWTNRAGAYVSDKMSFFEESRAFIDKYLGRWNAGDAQQQHQQGTASETDEQMDECTSGATSGEEVWGTPTSGGDNDDQDLQLINSENTHSSPTKSSTSLNDDDDTELMMDELLMAPPMTASTIRGLLPRRRLEPLFEEETESDEEKTQQDSDDIKKGEATTNGHYLEDSAGSSSDSESVPPAPPLVPRPVVPDREEDPSAEGQQENLAMEDQEDLHSSVERLLPNLATSAMGPRPLLTTRLLPTTAIDPQPPAVLVASSCEYLLDQRPSSVPGRDLAGGSIGTTTMTSSRPTPLSPAKAAATPIGMNATMTTKTTTTTMMTPSRAPSSTISTISSASDTTSSGGRASGRPPRFVPPPPPPRRLLLTQTDLIAGQAKTELPRKKSGATADSGCSVAVGDAASASDPTAIGSRTMPSTPSTSSLASAVAPKRPSSTIIETCLLGAPRPASATSSATASRMAKPIRQSPPTQHQSQPRGQSCPAAVPAPSTAAPDAPTVPMPHAGCGLSPRLEMRLALNHDILGDEDLICYEPGPDLTTILGHDLSTFHRLTGRDLLSRSATNRVQPKEAVISYSQQRNSKMDTPTVNRRPRPLSAGALGGSTSNSNPNNSRSSSSHGNSPLGGGLTRAGVEPVGQQQLVVQAGGGSAGRVGGGSGSAGGNSTACSSNRGGSKLGDLEILARREKIYSMSQSRSGCRVRETTSTTTLMAMATEMRSGMDSEKRDSSWPKRAAAAASLTRTRSSTSMDGAGAISTTTTTTTTMTEEAFLETEVGRSKRLINFIKRRNSEITASSTSSTPNFSDGAIGEQPQPSHSQSSLLLQKLPPGQSQAQDSVEMAQMSPRKDNDKPSLNRRLWKQITKRRRTNSVSQIVAG

Target ORF information:

RefSeq Version	NM_130645.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130645.6

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA AACGCAGCGA GTTCCTGGGC TGCAGCACTT TTCCACTGAA TGAGTTAGTG 
CATCCGGATT CGGGCGTCTC GGCAGGATCC TACAAGCTGC ATGCACAGGC ATGTCCTCCG 
CCTACTAGCC GCCATAGCCA ACCAAAAGCC AACGCAGGCC AGGATCAAAA GCAGGAAACG 
GAAGCGGCGA AGGATCAGGA TAAACAGCAG GACGATTCCC CCGCAGAAAT GGCAGCAGCC 
GTCACAGTCA CGCCCCAAAA ACCGGTTGCC ACTGGATCCG GTTCGGGATC GAATTCCGCA 
GCGATGGCAC TGAACGACGA GGTGATCAGC ATTAGCATCG ATAGCATGGG CAAAGAGGAT 
CCTCTGCTAC CCCAGCAGCC CCAGCAGCCC ATGAAGCTCA GCAAGAAGGC ACTCCACCAG 
CGAGATGCCG ACGAGAATCT CTTTTTAAGA TTCCTGGAGC TGGATCCGCC AGCGGATGGC 
AATGCAAACA GTACCACCAC CCAGGCACAA GCGACGGGCT CCCAGTCCTC GGCGTCGAAG 
GCCAACGAAA GCAATGCCAA CCACTTGAAC AATGGGACAT CCGGTGGACG CAGACAGTCC 
ACCATGCCAA ATAGCGGAGG ATCTAGCGTA GGAGGCGCTG TCCGCCAGCA GCAGGGACGT 
ACCCCATTCA CCATGACGAA AAGACTTACG CGAACCGAGG AACGGGGCTT TGGATTCTCT 
ATTGTCTGGA CGCATCCGCC ACGGGTGGAG AAAATCGAGG CGGGACTCTC GGCCGATCGG 
TGTGGCATTT TGCCCGGCGA CTATGTGATC TTTGTGGACA AGCATAACGT CGTCACGATG 
CCCGAGGCTG ATGTTCTGAA TTTGATACGA TCGCAGGGCT CGTCCTTGAC GTTGGAGATA 
TTCAGAAGGT CAGGCGCGGG CGCCACAACA ATCACGTCGA CGGACTTGGG CCAGAACAAT 
GTACACATAA GCACCAGATT GGGCGCGGCA GTGGGCTTGG GCAGCGAGGA GCACACCCTG 
GCCACCACCG CAACAGGAAC GACCACCGTA ATGAGTCTGC AAAGGACCAC CAGTACGAGG 
ATCCAGCCAT TGGCAAATAG CATGAGCCGG CCGGCTACCG CATGCTCGGG AACCACCTCC 
TCCATCGAGG CGGCCAAAAG GAGGCTTCAC CTGCCACAGG TCACCTTCAG CAAGGAGTCG 
ATTGTGCCTG TCACGGACAA CCGTAGGCGC TTCCTGCTCC AGCTGATTAG TCGGGAGCAG 
AACTTCACGG CTGCCCTGCA CTTCGGAGTG GACCGCTTTG TGCAGCCGCT TGGCGAACGA 
AAGGATCTGA TCTCGCCGAA CGATCATCGC ACTTTGTTCC AGAACATCGA CGAGCTGCTC 
AGGATTGCCG AGGACATCCT GGAGCAGTTG TGCAGCAGCG ATCAGCAGGA CCAGGAGCCA 
CAAATGAACT TTGCCTCGCG GGTCTACCTA TCAAAAACTA CAGCGATCTG TGCGGCATAC 
AAGAAGTACT GCAATGGAAT TAAGCGGGCC GACTGTGTTT TGGTCAATAA GTCCCGACAG 
ACTGGCTCCG AGTTTATAGC CTTCATTACG GAACCGGCCG TTCCACGTAA AAGACCCGAT 
CTCACCATGT TCATCCATCG ACCACTGCAG CATTTTCGTG AAATCCTTAA ACTGATGCAG 
TTGCTCGCTG GAAATTGCCA TGTGGATACG GAGGAGCACA AGAACTTTAG CACGGTTATC 
AACGAACTGC AGGCTGCCTA TCGGGAGATC ACTGTGAGCA GTGGTCTGAT GGAACCACTG 
GGAGAAGGTC GCCCCCTGCT CACCCTCCAG GATCTGGAGT CCCGGATGGT CTTTACCAAA 
TGCAAGCCTT TTACGTTGGC CGTTCAGGGG CGCCAGTGGA TCTTTGGCGG CGATTTGTCC 
CGGGTCGAAG GTCGCTCCGT TAAGCCCTAT TGGACACTAC TATTCAGTGA TATCATCGTT 
TTTGCCAAGG TCAGCCGTGA TCGAGTCCTG TTTATCACAG AGGAGCCCAT TCCCATCGCC 
AATGTGGTTG ACTCTTGTTT CCATATGCGC AAGAAAACCA CCGAGTTTCG TCTCACAGTG 
GATCCCAATG GACGCCTGGC GGAGAGTCCG ACGGGTTACT GTGCTCCCGA TCTCACCCGC 
ACTCCAAAGC GAGGAGCCCG CCGCAAGAGC CTTATTTTAA GGGCACCTTC GCTGGAACTT 
AAGGCCGTTT GGCAGAATCT TCTCCAGCGG CAGATCTTTC TAGTTAACGC CGCCCTTGGC 
TCCACGCCCT TGTCCAGTCC CTTGGACTCG CCGGATGTTC TCAACACCCT GGTGCCGCTG 
AGTGATATTG GTCTGACCAC CGCCTCCATG GGGTCGATGA AGTTACCATC GCTGGACAGC 
ATCCACCTGA AGCAGCAGCA GAAACAGCAG CGTACCAACA ATTCCCACGG ACACGTCCAT 
TCCGCCACAG GAGTGGCAAC CGATCGAACT GCGGATCGCT CGCACGGCCA CAACCATGGC 
ATCCACGGTG GTCATGTGGA ACAGATCGAG CTGCTGATCG ACGAGAAGTG TCGCATTCTG 
AACAAGACAG GCACTCCCAA GTCGAGCGCC TTGCATCTGG CCAACTGGAT GAAGGGTCAG 
CTGGACAAGC AGCAGCAGCA GGCTCGACTG GCGGCGATCG CGCGATCCCA GGAGAATGTG 
AGTGCCGAGG ACGAGCAACT GATTTTCAAC AGCGATAGCG AGCAGGACGA ACGGATCACC 
TACTGGACGC GTCAGCAGCT GGAGAAGCGT ACCAAGGAGC TCAATCTGGC CAAGGAAAAT 
GGCGGGCTAT CGGCTAGGCC CAATTTTGGG GGCAAGCGTC TGAGTGGCGT CGAGGAACTG 
AGCATGAGCG CCACCTCGGA TATCTACTCC ACGTCGGAGG CGGAGGGCGT GACCAGTGTG 
ATCAGTCAGT CGCATAGCAC CACATCCGAC AGCCAGATCA CCGTACGCTC TAGTCCCATT 
GTGCTGGACA AGCTGGCCGT TTGCCGTCAT TGCCACAAGA ATTGCCAGCA GAGCGGACCG 
GGTGGAGCGC GTCCTGGCGG CGGAGGCGGA GTCAACAACT CCAGTTCCAC GCCCGTCCTG 
CTCTGCAATT CGTTGAAAGT GCAGCACAGC CAATCGTCGC CAAATCGCTG CTGCAAGCTT 
AGCAATGGTA TTGCGGGAGC CACGGAAATG GCTAACCCGA AAAACAGCAA TCGAACTGGA 
ACTGGGACAG GAAGCGTGAC CAGCATGTCC AGCAGCACTA TTACGGGGGA ACTGTCCTCC 
AAAGTGGTGG CCAGTAGCAG TCGTCGGGAA ACCGGCGACA CCGAGAGCGA CGTGGCCCAA 
CTGATAACCG ATGAAATTTC CCAATCGCAA TCAGAGGCCA CCGATGACAG CGTCGGAGCG 
ATGCCCAATA GCAGTAGAAG TGCCGGAGAA GGAAACCCAG TTATCACCGG CTCCAGACTA 
CGTCATCTAG ATCCGCCAGT CTCAGTTTCA TCCTTGCCCA ATGGCCACTG CTCCGCTGGC 
GTCGGTGGTG GTTCGCCAAA ACCGCTGCCA CCACCACGTC GCACCCGCGT TGTGGCCCAA 
ATGTGCCAGG AGCCACCAAG GCCAAAGGCC AACCAGCAGG TGAAGGCCAT TGTGACCATT 
ACGGAGACGG CCAGGATAAT GCGGAGCAGT CCAACGAAAG GCTCGGGTTC GGGATCGGCA 
ACGGGCACGG GCTCGGTGGG TGGTTCCCCA GCCAAGTATG CCGCCTGCCA CTGTCGCTGT 
ACTCCAGAGG ACTTTACCCA CGCTCAACTC TCGCCGGACA AAATGGAGCA CCAGACCTGC 
AAGCTACTGG AATCACCCAA GCAAACGACC ACCACACTGG AGCAGAAACA GGAAGACGAG 
CAGTTGTCCC TGATGCTCAT TGGTCTGGCG CAGCTTGCTC CAGCTGCCAC ACTTTGTGGG 
CAGGAAAGGA TCCCCAAGGA GGAGAATAGT ACACCCACCA TAGCCGTGGT GCCGCCCACT 
CCCGATTCGG TGCTCACTAA AACCAGCACT CACGTTTGGG ACAACAGTGG CTGCTCGTCA 
AACGGCGGAG GAACCTCAAC CACCAGCACC GCTACCACTA CAACGAAACA ACCAAGGCAG 
GCCATTATTG AGAATATTCC AGAGGACTCG TGTGACGAGT CCCCATTGGA TGAGGAACCA 
CCCTATCGAC CCATGAGCAG TGCTCTTCGC CGATTCGGCA CCATGTCAAG CTTAGAGAAA 
CTACCTTCCG ATGATCGAAT GGATGAGGCC GATGAGCTAG ATGATGATTT GGATTCATTT 
AAGCCAAATG GCCACGACGG CAACAGTCCC CAAAACGAGG AAGACGACGA GGATGCGGAC 
TCGGAACGAG CTTTGGTACA AAACGACCTG GGGGCCTCCA GTTCGTCCGG AGTGATTGTC 
AATGGCGAGG GCCTGGCCAG TGGTGCGTGG ACAAATCGGG CGGGAGCCTA TGTCTCCGAC 
AAGATGTCCT TCTTCGAGGA ATCGCGCGCT TTCATTGATA AGTACTTGGG TCGCTGGAAT 
GCTGGCGATG CACAACAGCA GCACCAGCAG GGAACCGCCT CGGAGACGGA TGAGCAAATG 
GACGAGTGCA CCTCGGGAGC CACCAGTGGT GAAGAGGTCT GGGGCACACC CACCAGTGGC 
GGGGATAACG ATGATCAGGA CTTGCAGCTG ATCAACTCGG AAAACACACA TTCGTCGCCA 
ACCAAGTCGA GCACCTCCTT GAATGACGAC GACGACACGG AGTTGATGAT GGACGAGCTG 
TTAATGGCGC CACCTATGAC TGCCAGCACA ATTCGGGGAT TGTTGCCACG ACGTCGCCTT 
GAGCCCCTTT TTGAGGAGGA GACGGAGAGC GACGAAGAGA AGACGCAACA GGACAGCGAT 
GACATCAAAA AGGGGGAAGC AACGACCAAT GGCCACTACC TAGAGGATTC GGCTGGAAGT 
TCAAGCGACA GCGAGTCGGT GCCACCAGCC CCACCACTAG TTCCTCGGCC AGTGGTGCCG 
GATCGGGAGG AGGATCCGTC AGCGGAGGGC CAGCAGGAGA ATCTGGCGAT GGAGGACCAG 
GAGGACTTGC ACTCCAGCGT GGAGCGACTG CTTCCGAATC TGGCCACGAG TGCGATGGGG 
CCGCGTCCCT TACTCACCAC CCGTCTACTG CCCACCACGG CTATCGATCC TCAGCCGCCG 
GCAGTACTGG TGGCCTCATC CTGCGAGTAT CTGCTCGATC AGCGCCCCTC GAGCGTACCA 
GGACGCGATC TCGCCGGCGG GAGCATAGGC ACCACCACGA TGACATCCTC TCGGCCGACG 
CCTCTGTCTC CGGCGAAGGC AGCAGCGACA CCGATCGGGA TGAATGCGAC GATGACAACG 
AAGACGACGA CGACCACGAT GATGACGCCG AGTCGAGCTC CCTCATCGAC TATTTCAACA 
ATCAGCAGCG CGAGCGACAC TACCAGCTCC GGCGGCAGAG CCAGCGGCAG ACCTCCGAGA 
TTTGTACCGC CGCCACCGCC TCCGCGTCGC TTGCTCCTCA CGCAGACCGA TCTAATCGCT 
GGTCAGGCCA AAACGGAGCT ACCTCGAAAA AAATCTGGCG CTACTGCTGA CAGTGGCTGC 
TCGGTGGCTG TCGGCGACGC CGCCAGCGCT TCGGACCCAA CCGCTATCGG GTCAAGGACA 
ATGCCATCAA CACCGAGTAC CAGCAGCTTG GCGTCAGCGG TGGCCCCAAA ACGACCGTCA 
TCAACGATAA TAGAGACCTG CCTGCTGGGG GCACCCAGGC CCGCCTCAGC CACTTCATCA 
GCAACAGCCT CGAGAATGGC AAAGCCGATC CGCCAGAGTC CACCCACCCA GCACCAGTCG 
CAGCCGAGGG GTCAGAGCTG CCCGGCTGCA GTGCCAGCAC CATCGACGGC TGCGCCAGAC 
GCACCCACTG TCCCAATGCC CCACGCTGGA TGCGGCCTAA GCCCAAGGCT GGAAATGCGG 
CTGGCCCTCA ACCACGACAT TCTCGGGGAC GAGGACTTGA TCTGCTACGA GCCTGGTCCC 
GATCTAACGA CTATTCTGGG GCACGACCTC TCCACATTTC ATCGTCTGAC GGGTCGCGAT 
TTATTGAGTC GTTCAGCCAC GAACAGGGTG CAGCCAAAAG AGGCTGTCAT ATCGTACTCT 
CAACAACGCA ACTCAAAGAT GGACACGCCG ACGGTGAACC GCCGGCCCCG CCCCCTCTCA 
GCGGGCGCCT TGGGCGGCTC CACCAGCAAC AGCAATCCGA ACAACAGTCG CAGCAGCAGC 
AGCCACGGCA ACAGTCCGCT CGGCGGTGGT TTAACCCGTG CCGGCGTCGA GCCAGTGGGC 
CAACAGCAGC TGGTGGTTCA AGCAGGTGGT GGATCTGCTG GTCGAGTAGG CGGCGGGAGC 
GGAAGCGCCG GCGGCAACAG CACCGCCTGC AGCTCCAACA GAGGCGGGAG CAAATTAGGC 
GATCTGGAAA TCTTAGCGAG ACGCGAGAAG ATATACTCCA TGTCTCAATC GAGGAGTGGC 
TGTCGAGTCA GGGAGACGAC GTCCACGACC ACTCTGATGG CCATGGCTAC GGAGATGCGT 
TCGGGGATGG ATTCGGAGAA ACGCGACTCT TCCTGGCCCA AACGAGCAGC TGCAGCAGCC 
TCTCTGACGA GGACGAGGAG TTCTACTTCG ATGGACGGAG CGGGAGCCAT CAGTACCACA 
ACCACAACCA CCACAACAAT GACTGAGGAG GCCTTCCTTG AGACCGAAGT GGGCAGATCT 
AAGCGCTTGA TAAACTTTAT AAAGCGTCGC AACTCCGAGA TAACCGCCAG TAGCACCAGC 
AGCACCCCCA ACTTCTCCGA CGGCGCCATT GGCGAGCAGC CCCAACCGAG CCACAGCCAG 
AGCAGTCTTC TTCTCCAGAA GCTGCCGCCG GGTCAATCGC AGGCCCAGGA CTCAGTGGAA 
ATGGCCCAAA TGTCGCCGCG CAAGGATAAC GACAAGCCAT CGCTGAATCG CCGCCTCTGG 
AAGCAAATCA CCAAACGCAG ACGCACCAAC TCCGTGTCCC AAATAGTCGC AGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PsGEF ( NM_206610.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996333.4 Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant E, mRNA.

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CloneID	OFa00600
Clone ID Related Accession (Same CDS sequence)	NM_206610.5
Accession Version	NM_206610.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Protostome-specific GEF, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_206610.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA ACTCAGTTCC CGTTACTCAG TCCGAGTGGC AAGCTCGCGT GAAAAGCGGC 
ACAGAACGGC GAACGGCAGA ACGGCTGGCG AAGCTCGAAA ACGCAGGAAA CGAGGCAAAA 
CGCAGCGAGT TCCTGGGCTG CAGCACTTTT CCACTGAATG AGTTAGTGCA TCCGGATTCG 
GGCGTCTCGG CAGGATCCTA CAAGCTGCAT GCACAGGCAT GTCCTCCGCC TACTAGCCGC 
CATAGCCAAC CAAAAGCCAA CGCAGGCCAG GATCAAAAGC AGGAAACGGA AGCGGCGAAG 
GATCAGGATA AACAGCAGGA CGATTCCCCC GCAGAAATGG CAGCAGCCGT CACAGTCACG 
CCCCAAAAAC CGGTTGCCAC TGGATCCGGT TCGGGATCGA ATTCCGCAGC GATGGCACTG 
AACGACGAGG TGATCAGCAT TAGCATCGAT AGCATGGGCA AAGAGGATCC TCTGCTACCC 
CAGCAGCCCC AGCAGCCCAT GAAGCTCAGC AAGAAGGCAC TCCACCAGCG AGATGCCGAC 
GAGAATCTCT TTTTAAGATT CCTGGAGCTG GATCCGCCAG CGGATGGCAA TGCAAACAGT 
ACCACCACCC AGGCACAAGC GACGGGCTCC CAGTCCTCGG CGTCGAAGGC CAACGAAAGC 
AATGCCAACC ACTTGAACAA TGGGACATCC GGTGGACGCA GACAGTCCAC CATGCCAAAT 
AGCGGAGGAT CTAGCGTAGG AGGCGCTGTC CGCCAGCAGC AGGGACGTAC CCCATTCACC 
ATGACGAAAA GACTTACGCG AACCGAGGAA CGGGGCTTTG GATTCTCTAT TGTCTGGACG 
CATCCGCCAC GGGTGGAGAA AATCGAGGCG GGACTCTCGG CCGATCGGTG TGGCATTTTG 
CCCGGCGACT ATGTGATCTT TGTGGACAAG CATAACGTCG TCACGATGCC CGAGGCTGAT 
GTTCTGAATT TGATACGATC GCAGGGCTCG TCCTTGACGT TGGAGATATT CAGAAGGTCA 
GGCGCGGGCG CCACAACAAT CACGTCGACG GACTTGGGCC AGAACAATGT ACACATAAGC 
ACCAGATTGG GCGCGGCAGT GGGCTTGGGC AGCGAGGAGC ACACCCTGGC CACCACCGCA 
ACAGGAACGA CCACCGTAAT GAGTCTGCAA AGGACCACCA GTACGAGGAT CCAGCCATTG 
GCAAATAGCA TGAGCCGGCC GGCTACCGCA TGCTCGGGAA CCACCTCCTC CATCGAGGCG 
GCCAAAAGGA GGCTTCACCT GCCACAGGTC ACCTTCAGCA AGGAGTCGAT TGTGCCTGTC 
ACGGACAACC GTAGGCGCTT CCTGCTCCAG CTGATTAGTC GGGAGCAGAA CTTCACGGCT 
GCCCTGCACT TCGGAGTGGA CCGCTTTGTG CAGCCGCTTG GCGAACGAAA GGATCTGATC 
TCGCCGAACG ATCATCGCAC TTTGTTCCAG AACATCGACG AGCTGCTCAG GATTGCCGAG 
GACATCCTGG AGCAGTTGTG CAGCAGCGAT CAGCAGGACC AGGAGCCACA AATGAACTTT 
GCCTCGCGGG TCTACCTATC AAAAACTACA GCGATCTGTG CGGCATACAA GAAGTACTGC 
AATGGAATTA AGCGGGCCGA CTGTGTTTTG GTCAATAAGT CCCGACAGAC TGGCTCCGAG 
TTTATAGCCT TCATTACGGA ACCGGCCGTT CCACGTAAAA GACCCGATCT CACCATGTTC 
ATCCATCGAC CACTGCAGCA TTTTCGTGAA ATCCTTAAAC TGATGCAGTT GCTCGCTGGA 
AATTGCCATG TGGATACGGA GGAGCACAAG AACTTTAGCA CGGTTATCAA CGAACTGCAG 
GCTGCCTATC GGGAGATCAC TGTGAGCAGT GGTCTGATGG AACCACTGGG AGAAGGTCGC 
CCCCTGCTCA CCCTCCAGGA TCTGGAGTCC CGGATGGTCT TTACCAAATG CAAGCCTTTT 
ACGTTGGCCG TTCAGGGGCG CCAGTGGATC TTTGGCGGCG ATTTGTCCCG GGTCGAAGGT 
CGCTCCGTTA AGCCCTATTG GACACTACTA TTCAGTGATA TCATCGTTTT TGCCAAGGTC 
AGCCGTGATC GAGTCCTGTT TATCACAGAG GAGCCCATTC CCATCGCCAA TGTGGTTGAC 
TCTTGTTTCC ATATGCGCAA GAAAACCACC GAGTTTCGTC TCACAGTGGA TCCCAATGGA 
CGCCTGGCGG AGAGTCCGAC GGGTTACTGT GCTCCCGATC TCACCCGCAC TCCAAAGCGA 
GGAGCCCGCC GCAAGAGCCT TATTTTAAGG GCACCTTCGC TGGAACTTAA GGCCGTTTGG 
CAGAATCTTC TCCAGCGGCA GATCTTTCTA GTTAACGCCG CCCTTGGCTC CACGCCCTTG 
TCCAGTCCCT TGGACTCGCC GGATGTTCTC AACACCCTGG TGCCGCTGAG TGATATTGGT 
CTGACCACCG CCTCCATGGG GTCGATGAAG TTACCATCGC TGGACAGCAT CCACCTGAAG 
CAGCAGCAGA AACAGCAGCG TACCAACAAT TCCCACGGAC ACGTCCATTC CGCCACAGGA 
GTGGCAACCG ATCGAACTGC GGATCGCTCG CACGGCCACA ACCATGGCAT CCACGGTGGT 
CATGTGGAAC AGATCGAGCT GCTGATCGAC GAGAAGTGTC GCATTCTGAA CAAGACAGGC 
ACTCCCAAGT CGAGCGCCTT GCATCTGGCC AACTGGATGA AGGGTCAGCT GGACAAGCAG 
CAGCAGCAGG CTCGACTGGC GGCGATCGCG CGATCCCAGG AGAATGTGAG TGCCGAGGAC 
GAGCAACTGA TTTTCAACAG CGATAGCGAG CAGGACGAAC GGATCACCTA CTGGACGCGT 
CAGCAGCTGG AGAAGCGTAC CAAGGAGCTC AATCTGGCCA AGGAAAATGG CGGGCTATCG 
GCTAGGCCCA ATTTTGGGGG CAAGCGTCTG AGTGGCGTCG AGGAACTGAG CATGAGCGCC 
ACCTCGGATA TCTACTCCAC GTCGGAGGCG GAGGGCGTGA CCAGTGTGAT CAGTCAGTCG 
CATAGCACCA CATCCGACAG CCAGATCACC GTACGCTCTA GTCCCATTGT GCTGGACAAG 
CTGGCCGTTT GCCGTCATTG CCACAAGAAT TGCCAGCAGA GCGGACCGGG TGGAGCGCGT 
CCTGGCGGCG GAGGCGGAGT CAACAACTCC AGTTCCACGC CCGTCCTGCT CTGCAATTCG 
TTGAAAGTGC AGCACAGCCA ATCGTCGCCA AATCGCTGCT GCAAGCTTAG CAATGGTATT 
GCGGGAGCCA CGGAAATGGC TAACCCGAAA AACAGCAATC GAACTGGAAC TGGGACAGGA 
AGCGTGACCA GCATGTCCAG CAGCACTATT ACGGGGGAAC TGTCCTCCAA AGTGGTGGCC 
AGTAGCAGTC GTCGGGAAAC CGGCGACACC GAGAGCGACG TGGCCCAACT GATAACCGAT 
GAAATTTCCC AATCGCAATC AGAGGCCACC GATGACAGCG TCGGAGCGAT GCCCAATAGC 
AGTAGAAGTG CCGGAGAAGG AAACCCAGTT ATCACCGGCT CCAGACTACG TCATCTAGAT 
CCGCCAGTCT CAGTTTCATC CTTGCCCAAT GGCCACTGCT CCGCTGGCGT CGGTGGTGGT 
TCGCCAAAAC CGCTGCCACC ACCACGTCGC ACCCGCGTTG TGGCCCAAAT GTGCCAGGAG 
CCACCAAGGC CAAAGGCCAA CCAGCAGGTG AAGGCCATTG TGACCATTAC GGAGACGGCC 
AGGATAATGC GGAGCAGTCC AACGAAAGGC TCGGGTTCGG GATCGGCAAC GGGCACGGGC 
TCGGTGGGTG GTTCCCCAGC CAAGTATGCC GCCTGCCACT GTCGCTGTAC TCCAGAGGAC 
TTTACCCACG CTCAACTCTC GCCGGACAAA ATGGAGCACC AGACCTGCAA GCTACTGGAA 
TCACCCAAGC AAACGACCAC CACACTGGAG CAGAAACAGG AAGACGAGCA GTTGTCCCTG 
ATGCTCATTG GTCTGGCGCA GCTTGCTCCA GCTGCCACAC TTTGTGGGCA GGAAAGGATC 
CCCAAGGAGG AGAATAGTAC ACCCACCATA GCCGTGGTGC CGCCCACTCC CGATTCGGTG 
CTCACTAAAA CCAGCACTCA CGTTTGGGAC AACAGTGGCT GCTCGTCAAA CGGCGGAGGA 
ACCTCAACCA CCAGCACCGC TACCACTACA ACGAAACAAC CAAGGCAGGC CATTATTGAG 
AATATTCCAG AGGACTCGTG TGACGAGTCC CCATTGGATG AGGAACCACC CTATCGACCC 
ATGAGCAGTG CTCTTCGCCG ATTCGGCACC ATGTCAAGCT TAGAGAAACT ACCTTCCGAT 
GATCGAATGG ATGAGGCCGA TGAGCTAGAT GATGATTTGG ATTCATTTAA GCCAAATGGC 
CACGACGGCA ACAGTCCCCA AAACGAGGAA GACGACGAGG ATGCGGACTC GGAACGAGCT 
TTGGTACAAA ACGACCTGGG GGCCTCCAGT TCGTCCGGAG TGATTGTCAA TGGCGAGGGC 
CTGGCCAGTG GTGCGTGGAC AAATCGGGCG GGAGCCTATG TCTCCGACAA GATGTCCTTC 
TTCGAGGAAT CGCGCGCTTT CATTGATAAG TACTTGGGTC GCTGGAATGC TGGCGATGCA 
CAACAGCAGC ACCAGCAGGG AACCGCCTCG GAGACGGATG AGCAAATGGA CGAGTGCACC 
TCGGGAGCCA CCAGTGGTGA AGAGGTCTGG GGCACACCCA CCAGTGGCGG GGATAACGAT 
GATCAGGACT TGCAGCTGAT CAACTCGGAA AACACACATT CGTCGCCAAC CAAGTCGAGC 
ACCTCCTTGA ATGACGACGA CGACACGGAG TTGATGATGG ACGAGCTGTT AATGGCGCCA 
CCTATGACTG CCAGCACAAT TCGGGGATTG TTGCCACGCT TTTACAGACG TCGCCTTGAG 
CCCCTTTTTG AGGAGGAGAC GGAGAGCGAC GAAGAGAAGA CGCAACAGGA CAGCGATGAC 
ATCAAAAAGG GGGAAGCAAC GACCAATGGC CACTACCTAG AGGATTCGGC TGGAAGTTCA 
AGCGACAGCG AGTCGGTGCC ACCAGCCCCA CCACTAGTTC CTCGGCCAGT GGTGCCGGAT 
CGGGAGGAGG ATCCGTCAGC GGAGGGCCAG CAGGAGAATC TGGCGATGGA GGACCAGGAG 
GACTTGCACT CCAGCGTGGA GCGACTGCTT CCGAATCTGG CCACGAGTGC GATGGGGCCG 
CGTCCCTTAC TCACCACCCG TCTACTGCCC ACCACGGCTA TCGATCCTCA GCCGCCGGCA 
GTACTGGTGG CCTCATCCTG CGAGTATCTG CTCGATCAGC GCCCCTCGAG CGTACCAGGA 
CGCGATCTCG CCGGCGGGAG CATAGGCACC ACCACGATGA CATCCTCTCG GCCGACGCCT 
CTGTCTCCGG CGAAGGCAGC AGCGACACCG ATCGGGATGA ATGCGACGAT GACAACGAAG 
ACGACGACGA CCACGATGAT GACGCCGAGT CGAGCTCCCT CATCGACTAT TTCAACAATC 
AGCAGCGCGA GCGACACTAC CAGCTCCGGC GGCAGAGCCA GCGGCAGACC TCCGAGATTT 
GTACCGCCGC CACCGCCTCC GCGTCGCTTG CTCCTCACGC AGACCGATCT AATCGCTGGT 
CAGGCCAAAA CGGAGCTACC TCGAAAAAAA TCTGGCGCTA CTGCTGACAG TGGCTGCTCG 
GTGGCTGTCG GCGACGCCGC CAGCGCTTCG GACCCAACCG CTATCGGGTC AAGGACAATG 
CCATCAACAC CGAGTACCAG CAGCTTGGCG TCAGCGGTGG CCCCAAAACG ACCGTCATCA 
ACGATAATAG AGACCTGCCT GCTGGGGGCA CCCAGGCCCG CCTCAGCCAC TTCATCAGCA 
ACAGCCTCGA GAATGGCAAA GCCGATCCGC CAGAGTCCAC CCACCCAGCA CCAGTCGCAG 
CCGAGGGGTC AGAGCTGCCC GGCTGCAGTG CCAGCACCAT CGACGGCTGC GCCAGACGCA 
CCCACTGTCC CAATGCCCCA CGCTGGATGC GGCCTAAGCC CAAGGCTGGA AATGCGGCTG 
GCCCTCAACC ACGACATTCT CGGGGACGAG GACTTGATCT GCTACGAGCC TGGTCCCGAT 
CTAACGACTA TTCTGGGGCA CGACCTCTCC ACATTTCATC GTCTGACGGG TCGCGATTTA 
TTGAGTCGTT CAGCCACGAA CAGGGTGCAG CCAAAAGAGG CTGTCATATC GTACTCTCAA 
CAACGCAACT CAAAGATGGA CACGCCGACG GTGAACCGCC GGCCCCGCCC CCTCTCAGCG 
GGCGCCTTGG GCGGCTCCAC CAGCAACAGC AATCCGAACA ACAGTCGCAG CAGCAGCAGC 
CACGGCAACA GTCCGCTCGG CGGTGGTTTA ACCCGTGCCG GCGTCGAGCC AGTGGGCCAA 
CAGCAGCTGG TGGTTCAAGC AGGTGGTGGA TCTGCTGGTC GAGTAGGCGG CGGGAGCGGA 
AGCGCCGGCG GCAACAGCAC CGCCTGCAGC TCCAACAGAG GCGGGAGCAA ATTAGGCGAT 
CTGGAAATCT TAGCGAGACG CGAGAAGATA TACTCCATGT CTCAATCGAG GAGTGGCTGT 
CGAGTCAGGG AGACGACGTC CACGACCACT CTGATGGCCA TGGCTACGGA GATGCGTTCG 
GGGATGGATT CGGAGAAACG CGACTCTTCC TGGCCCAAAC GAGCAGCTGC AGCAGCCTCT 
CTGACGAGGA CGAGGAGTTC TACTTCGATG GACGGAGCGG GAGCCATCAG TACCACAACC 
ACAACCACCA CAACAATGAC TGAGGAGGCC TTCCTTGAGA CCGAAGTGGG CAGATCTAAG 
CGCTTGATAA ACTTTATAAA GCGTCGCAAC TCCGAGATAA CCGCCAGTAG CACCAGCAGC 
ACCCCCAACT TCTCCGACGG CGCCATTGGC GAGCAGCCCC AACCGAGCCA CAGCCAGAGC 
AGTCTTCTTC TCCAGAAGCT GCCGCCGGGT CAATCGCAGG CCCAGGACTC AGTGGAAATG 
GCCCAAATGT CGCCGCGCAA GGATAACGAC AAGCCATCGC TGAATCGCCG CCTCTGGAAG 
CAAATCACCA AACGCAGACG CACCAACTCC GTGTCCCAAA TAGTCGCAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996333.4
CDS	260..8593
Translation	MPTMTRMHRHSSSSAVVEESRGRRRGVGVPGVGDANKENFGVHFMSSPFGNASLIALQDLSNVHGKSPQRRSFSEGSGPRQATPQLAALRCLPRTTGGAVALEDSQLSSSRMGDTTLDRMLDAIIESARKEVRCTKTLPGAGATTSTTILADNAEWSETSVHEMEVRTPTHLKRQRVVRRKNPHKTTTINQTQSHQAKKLEPLQLVPSTKRCLSFSSSSASSDLDEDEQQVAKRSSLASPTTTPPSHCTTTTSSISSSSSSNGGADMEASQRGSIDVSIAFDAKEQQLNVHVIRCRDLQRSHGSGNGSINAYVKVALSGGAQPPGYGGHSSGGSMSSGYQRTAVHRHSGRPYFDQRFNFQISSGEETAGQYLQLAVWHRDRHLNSVPVTQSEWQARVKSGTERRTAERLAKLENAGNEAKRSEFLGCSTFPLNELVHPDSGVSAGSYKLHAQACPPPTSRHSQPKANAGQDQKQETEAAKDQDKQQDDSPAEMAAAVTVTPQKPVATGSGSGSNSAAMALNDEVISISIDSMGKEDPLLPQQPQQPMKLSKKALHQRDADENLFLRFLELDPPADGNANSTTTQAQATGSQSSASKANESNANHLNNGTSGGRRQSTMPNSGGSSVGGAVRQQQGRTPFTMTKRLTRTEERGFGFSIVWTHPPRVEKIEAGLSADRCGILPGDYVIFVDKHNVVTMPEADVLNLIRSQGSSLTLEIFRRSGAGATTITSTDLGQNNVHISTRLGAAVGLGSEEHTLATTATGTTTVMSLQRTTSTRIQPLANSMSRPATACSGTTSSIEAAKRRLHLPQVTFSKESIVPVTDNRRRFLLQLISREQNFTAALHFGVDRFVQPLGERKDLISPNDHRTLFQNIDELLRIAEDILEQLCSSDQQDQEPQMNFASRVYLSKTTAICAAYKKYCNGIKRADCVLVNKSRQTGSEFIAFITEPAVPRKRPDLTMFIHRPLQHFREILKLMQLLAGNCHVDTEEHKNFSTVINELQAAYREITVSSGLMEPLGEGRPLLTLQDLESRMVFTKCKPFTLAVQGRQWIFGGDLSRVEGRSVKPYWTLLFSDIIVFAKVSRDRVLFITEEPIPIANVVDSCFHMRKKTTEFRLTVDPNGRLAESPTGYCAPDLTRTPKRGARRKSLILRAPSLELKAVWQNLLQRQIFLVNAALGSTPLSSPLDSPDVLNTLVPLSDIGLTTASMGSMKLPSLDSIHLKQQQKQQRTNNSHGHVHSATGVATDRTADRSHGHNHGIHGGHVEQIELLIDEKCRILNKTGTPKSSALHLANWMKGQLDKQQQQARLAAIARSQENVSAEDEQLIFNSDSEQDERITYWTRQQLEKRTKELNLAKENGGLSARPNFGGKRLSGVEELSMSATSDIYSTSEAEGVTSVISQSHSTTSDSQITVRSSPIVLDKLAVCRHCHKNCQQSGPGGARPGGGGGVNNSSSTPVLLCNSLKVQHSQSSPNRCCKLSNGIAGATEMANPKNSNRTGTGTGSVTSMSSSTITGELSSKVVASSSRRETGDTESDVAQLITDEISQSQSEATDDSVGAMPNSSRSAGEGNPVITGSRLRHLDPPVSVSSLPNGHCSAGVGGGSPKPLPPPRRTRVVAQMCQEPPRPKANQQVKAIVTITETARIMRSSPTKGSGSGSATGTGSVGGSPAKYAACHCRCTPEDFTHAQLSPDKMEHQTCKLLESPKQTTTTLEQKQEDEQLSLMLIGLAQLAPAATLCGQERIPKEENSTPTIAVVPPTPDSVLTKTSTHVWDNSGCSSNGGGTSTTSTATTTTKQPRQAIIENIPEDSCDESPLDEEPPYRPMSSALRRFGTMSSLEKLPSDDRMDEADELDDDLDSFKPNGHDGNSPQNEEDDEDADSERALVQNDLGASSSSGVIVNGEGLASGAWTNRAGAYVSDKMSFFEESRAFIDKYLGRWNAGDAQQQHQQGTASETDEQMDECTSGATSGEEVWGTPTSGGDNDDQDLQLINSENTHSSPTKSSTSLNDDDDTELMMDELLMAPPMTASTIRGLLPRFYRRRLEPLFEEETESDEEKTQQDSDDIKKGEATTNGHYLEDSAGSSSDSESVPPAPPLVPRPVVPDREEDPSAEGQQENLAMEDQEDLHSSVERLLPNLATSAMGPRPLLTTRLLPTTAIDPQPPAVLVASSCEYLLDQRPSSVPGRDLAGGSIGTTTMTSSRPTPLSPAKAAATPIGMNATMTTKTTTTTMMTPSRAPSSTISTISSASDTTSSGGRASGRPPRFVPPPPPPRRLLLTQTDLIAGQAKTELPRKKSGATADSGCSVAVGDAASASDPTAIGSRTMPSTPSTSSLASAVAPKRPSSTIIETCLLGAPRPASATSSATASRMAKPIRQSPPTQHQSQPRGQSCPAAVPAPSTAAPDAPTVPMPHAGCGLSPRLEMRLALNHDILGDEDLICYEPGPDLTTILGHDLSTFHRLTGRDLLSRSATNRVQPKEAVISYSQQRNSKMDTPTVNRRPRPLSAGALGGSTSNSNPNNSRSSSSHGNSPLGGGLTRAGVEPVGQQQLVVQAGGGSAGRVGGGSGSAGGNSTACSSNRGGSKLGDLEILARREKIYSMSQSRSGCRVRETTSTTTLMAMATEMRSGMDSEKRDSSWPKRAAAAASLTRTRSSTSMDGAGAISTTTTTTTTMTEEAFLETEVGRSKRLINFIKRRNSEITASSTSSTPNFSDGAIGEQPQPSHSQSSLLLQKLPPGQSQAQDSVEMAQMSPRKDNDKPSLNRRLWKQITKRRRTNSVSQIVAG

Target ORF information:

RefSeq Version	NM_206610.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Protostome-specific GEF (PsGEF), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206610.5

ORF Insert Sequence:

ATGCCAACCA TGACACGGAT GCATCGCCAC TCCAGTTCCA GTGCCGTGGT GGAGGAGTCG 
CGTGGCCGGC GCCGTGGAGT GGGCGTGCCA GGAGTCGGAG ACGCGAACAA GGAGAATTTC 
GGGGTGCACT TCATGAGCAG TCCCTTCGGC AATGCCAGTT TGATTGCCCT GCAGGATTTG 
AGCAACGTGC ACGGAAAGAG TCCGCAGCGC AGGAGTTTCA GCGAGGGCAG TGGTCCGCGG 
CAGGCCACGC CACAATTGGC GGCACTGCGC TGCTTGCCAC GCACCACCGG TGGTGCAGTT 
GCTCTGGAGG ATTCTCAGCT CTCCTCATCG CGAATGGGCG ATACCACACT GGACCGCATG 
CTGGATGCCA TCATAGAGTC GGCCAGGAAG GAGGTGAGGT GTACGAAGAC ACTGCCAGGC 
GCCGGCGCCA CCACTTCGAC CACCATTTTG GCGGATAATG CCGAGTGGAG CGAGACCAGT 
GTCCACGAAA TGGAGGTGCG CACGCCCACC CACTTGAAGC GGCAGCGGGT GGTGCGGCGC 
AAGAACCCAC ACAAGACTAC CACCATTAAC CAAACGCAAA GTCACCAAGC CAAGAAGTTG 
GAGCCACTGC AGTTGGTTCC CAGCACCAAA CGCTGCCTGA GCTTCTCATC CAGTTCCGCC 
TCGAGTGATT TGGACGAGGA TGAGCAGCAG GTGGCCAAGA GGAGTTCGCT GGCCTCCCCC 
ACCACCACAC CTCCCTCCCA CTGCACCACC ACCACCAGCA GCATCAGCAG CAGCAGCAGC 
AGTAATGGTG GTGCGGACAT GGAGGCCAGT CAGCGGGGCA GCATCGATGT GAGCATCGCA 
TTCGATGCCA AGGAGCAGCA ACTTAATGTA CATGTGATTC GCTGTCGCGA CTTGCAGCGC 
TCCCATGGCA GTGGAAACGG CAGCATCAAC GCCTACGTCA AGGTGGCCCT GTCCGGAGGA 
GCCCAGCCAC CCGGATACGG TGGCCACTCG TCGGGGGGAA GCATGAGTTC CGGGTACCAA 
CGCACCGCCG TCCATCGGCA CTCGGGCCGC CCGTACTTCG ATCAGCGATT CAACTTCCAG 
ATTTCCAGTG GCGAGGAGAC CGCTGGACAG TATCTTCAGT TGGCCGTGTG GCATCGGGAT 
CGCCATTTAA ACTCAGTTCC CGTTACTCAG TCCGAGTGGC AAGCTCGCGT GAAAAGCGGC 
ACAGAACGGC GAACGGCAGA ACGGCTGGCG AAGCTCGAAA ACGCAGGAAA CGAGGCAAAA 
CGCAGCGAGT TCCTGGGCTG CAGCACTTTT CCACTGAATG AGTTAGTGCA TCCGGATTCG 
GGCGTCTCGG CAGGATCCTA CAAGCTGCAT GCACAGGCAT GTCCTCCGCC TACTAGCCGC 
CATAGCCAAC CAAAAGCCAA CGCAGGCCAG GATCAAAAGC AGGAAACGGA AGCGGCGAAG 
GATCAGGATA AACAGCAGGA CGATTCCCCC GCAGAAATGG CAGCAGCCGT CACAGTCACG 
CCCCAAAAAC CGGTTGCCAC TGGATCCGGT TCGGGATCGA ATTCCGCAGC GATGGCACTG 
AACGACGAGG TGATCAGCAT TAGCATCGAT AGCATGGGCA AAGAGGATCC TCTGCTACCC 
CAGCAGCCCC AGCAGCCCAT GAAGCTCAGC AAGAAGGCAC TCCACCAGCG AGATGCCGAC 
GAGAATCTCT TTTTAAGATT CCTGGAGCTG GATCCGCCAG CGGATGGCAA TGCAAACAGT 
ACCACCACCC AGGCACAAGC GACGGGCTCC CAGTCCTCGG CGTCGAAGGC CAACGAAAGC 
AATGCCAACC ACTTGAACAA TGGGACATCC GGTGGACGCA GACAGTCCAC CATGCCAAAT 
AGCGGAGGAT CTAGCGTAGG AGGCGCTGTC CGCCAGCAGC AGGGACGTAC CCCATTCACC 
ATGACGAAAA GACTTACGCG AACCGAGGAA CGGGGCTTTG GATTCTCTAT TGTCTGGACG 
CATCCGCCAC GGGTGGAGAA AATCGAGGCG GGACTCTCGG CCGATCGGTG TGGCATTTTG 
CCCGGCGACT ATGTGATCTT TGTGGACAAG CATAACGTCG TCACGATGCC CGAGGCTGAT 
GTTCTGAATT TGATACGATC GCAGGGCTCG TCCTTGACGT TGGAGATATT CAGAAGGTCA 
GGCGCGGGCG CCACAACAAT CACGTCGACG GACTTGGGCC AGAACAATGT ACACATAAGC 
ACCAGATTGG GCGCGGCAGT GGGCTTGGGC AGCGAGGAGC ACACCCTGGC CACCACCGCA 
ACAGGAACGA CCACCGTAAT GAGTCTGCAA AGGACCACCA GTACGAGGAT CCAGCCATTG 
GCAAATAGCA TGAGCCGGCC GGCTACCGCA TGCTCGGGAA CCACCTCCTC CATCGAGGCG 
GCCAAAAGGA GGCTTCACCT GCCACAGGTC ACCTTCAGCA AGGAGTCGAT TGTGCCTGTC 
ACGGACAACC GTAGGCGCTT CCTGCTCCAG CTGATTAGTC GGGAGCAGAA CTTCACGGCT 
GCCCTGCACT TCGGAGTGGA CCGCTTTGTG CAGCCGCTTG GCGAACGAAA GGATCTGATC 
TCGCCGAACG ATCATCGCAC TTTGTTCCAG AACATCGACG AGCTGCTCAG GATTGCCGAG 
GACATCCTGG AGCAGTTGTG CAGCAGCGAT CAGCAGGACC AGGAGCCACA AATGAACTTT 
GCCTCGCGGG TCTACCTATC AAAAACTACA GCGATCTGTG CGGCATACAA GAAGTACTGC 
AATGGAATTA AGCGGGCCGA CTGTGTTTTG GTCAATAAGT CCCGACAGAC TGGCTCCGAG 
TTTATAGCCT TCATTACGGA ACCGGCCGTT CCACGTAAAA GACCCGATCT CACCATGTTC 
ATCCATCGAC CACTGCAGCA TTTTCGTGAA ATCCTTAAAC TGATGCAGTT GCTCGCTGGA 
AATTGCCATG TGGATACGGA GGAGCACAAG AACTTTAGCA CGGTTATCAA CGAACTGCAG 
GCTGCCTATC GGGAGATCAC TGTGAGCAGT GGTCTGATGG AACCACTGGG AGAAGGTCGC 
CCCCTGCTCA CCCTCCAGGA TCTGGAGTCC CGGATGGTCT TTACCAAATG CAAGCCTTTT 
ACGTTGGCCG TTCAGGGGCG CCAGTGGATC TTTGGCGGCG ATTTGTCCCG GGTCGAAGGT 
CGCTCCGTTA AGCCCTATTG GACACTACTA TTCAGTGATA TCATCGTTTT TGCCAAGGTC 
AGCCGTGATC GAGTCCTGTT TATCACAGAG GAGCCCATTC CCATCGCCAA TGTGGTTGAC 
TCTTGTTTCC ATATGCGCAA GAAAACCACC GAGTTTCGTC TCACAGTGGA TCCCAATGGA 
CGCCTGGCGG AGAGTCCGAC GGGTTACTGT GCTCCCGATC TCACCCGCAC TCCAAAGCGA 
GGAGCCCGCC GCAAGAGCCT TATTTTAAGG GCACCTTCGC TGGAACTTAA GGCCGTTTGG 
CAGAATCTTC TCCAGCGGCA GATCTTTCTA GTTAACGCCG CCCTTGGCTC CACGCCCTTG 
TCCAGTCCCT TGGACTCGCC GGATGTTCTC AACACCCTGG TGCCGCTGAG TGATATTGGT 
CTGACCACCG CCTCCATGGG GTCGATGAAG TTACCATCGC TGGACAGCAT CCACCTGAAG 
CAGCAGCAGA AACAGCAGCG TACCAACAAT TCCCACGGAC ACGTCCATTC CGCCACAGGA 
GTGGCAACCG ATCGAACTGC GGATCGCTCG CACGGCCACA ACCATGGCAT CCACGGTGGT 
CATGTGGAAC AGATCGAGCT GCTGATCGAC GAGAAGTGTC GCATTCTGAA CAAGACAGGC 
ACTCCCAAGT CGAGCGCCTT GCATCTGGCC AACTGGATGA AGGGTCAGCT GGACAAGCAG 
CAGCAGCAGG CTCGACTGGC GGCGATCGCG CGATCCCAGG AGAATGTGAG TGCCGAGGAC 
GAGCAACTGA TTTTCAACAG CGATAGCGAG CAGGACGAAC GGATCACCTA CTGGACGCGT 
CAGCAGCTGG AGAAGCGTAC CAAGGAGCTC AATCTGGCCA AGGAAAATGG CGGGCTATCG 
GCTAGGCCCA ATTTTGGGGG CAAGCGTCTG AGTGGCGTCG AGGAACTGAG CATGAGCGCC 
ACCTCGGATA TCTACTCCAC GTCGGAGGCG GAGGGCGTGA CCAGTGTGAT CAGTCAGTCG 
CATAGCACCA CATCCGACAG CCAGATCACC GTACGCTCTA GTCCCATTGT GCTGGACAAG 
CTGGCCGTTT GCCGTCATTG CCACAAGAAT TGCCAGCAGA GCGGACCGGG TGGAGCGCGT 
CCTGGCGGCG GAGGCGGAGT CAACAACTCC AGTTCCACGC CCGTCCTGCT CTGCAATTCG 
TTGAAAGTGC AGCACAGCCA ATCGTCGCCA AATCGCTGCT GCAAGCTTAG CAATGGTATT 
GCGGGAGCCA CGGAAATGGC TAACCCGAAA AACAGCAATC GAACTGGAAC TGGGACAGGA 
AGCGTGACCA GCATGTCCAG CAGCACTATT ACGGGGGAAC TGTCCTCCAA AGTGGTGGCC 
AGTAGCAGTC GTCGGGAAAC CGGCGACACC GAGAGCGACG TGGCCCAACT GATAACCGAT 
GAAATTTCCC AATCGCAATC AGAGGCCACC GATGACAGCG TCGGAGCGAT GCCCAATAGC 
AGTAGAAGTG CCGGAGAAGG AAACCCAGTT ATCACCGGCT CCAGACTACG TCATCTAGAT 
CCGCCAGTCT CAGTTTCATC CTTGCCCAAT GGCCACTGCT CCGCTGGCGT CGGTGGTGGT 
TCGCCAAAAC CGCTGCCACC ACCACGTCGC ACCCGCGTTG TGGCCCAAAT GTGCCAGGAG 
CCACCAAGGC CAAAGGCCAA CCAGCAGGTG AAGGCCATTG TGACCATTAC GGAGACGGCC 
AGGATAATGC GGAGCAGTCC AACGAAAGGC TCGGGTTCGG GATCGGCAAC GGGCACGGGC 
TCGGTGGGTG GTTCCCCAGC CAAGTATGCC GCCTGCCACT GTCGCTGTAC TCCAGAGGAC 
TTTACCCACG CTCAACTCTC GCCGGACAAA ATGGAGCACC AGACCTGCAA GCTACTGGAA 
TCACCCAAGC AAACGACCAC CACACTGGAG CAGAAACAGG AAGACGAGCA GTTGTCCCTG 
ATGCTCATTG GTCTGGCGCA GCTTGCTCCA GCTGCCACAC TTTGTGGGCA GGAAAGGATC 
CCCAAGGAGG AGAATAGTAC ACCCACCATA GCCGTGGTGC CGCCCACTCC CGATTCGGTG 
CTCACTAAAA CCAGCACTCA CGTTTGGGAC AACAGTGGCT GCTCGTCAAA CGGCGGAGGA 
ACCTCAACCA CCAGCACCGC TACCACTACA ACGAAACAAC CAAGGCAGGC CATTATTGAG 
AATATTCCAG AGGACTCGTG TGACGAGTCC CCATTGGATG AGGAACCACC CTATCGACCC 
ATGAGCAGTG CTCTTCGCCG ATTCGGCACC ATGTCAAGCT TAGAGAAACT ACCTTCCGAT 
GATCGAATGG ATGAGGCCGA TGAGCTAGAT GATGATTTGG ATTCATTTAA GCCAAATGGC 
CACGACGGCA ACAGTCCCCA AAACGAGGAA GACGACGAGG ATGCGGACTC GGAACGAGCT 
TTGGTACAAA ACGACCTGGG GGCCTCCAGT TCGTCCGGAG TGATTGTCAA TGGCGAGGGC 
CTGGCCAGTG GTGCGTGGAC AAATCGGGCG GGAGCCTATG TCTCCGACAA GATGTCCTTC 
TTCGAGGAAT CGCGCGCTTT CATTGATAAG TACTTGGGTC GCTGGAATGC TGGCGATGCA 
CAACAGCAGC ACCAGCAGGG AACCGCCTCG GAGACGGATG AGCAAATGGA CGAGTGCACC 
TCGGGAGCCA CCAGTGGTGA AGAGGTCTGG GGCACACCCA CCAGTGGCGG GGATAACGAT 
GATCAGGACT TGCAGCTGAT CAACTCGGAA AACACACATT CGTCGCCAAC CAAGTCGAGC 
ACCTCCTTGA ATGACGACGA CGACACGGAG TTGATGATGG ACGAGCTGTT AATGGCGCCA 
CCTATGACTG CCAGCACAAT TCGGGGATTG TTGCCACGCT TTTACAGACG TCGCCTTGAG 
CCCCTTTTTG AGGAGGAGAC GGAGAGCGAC GAAGAGAAGA CGCAACAGGA CAGCGATGAC 
ATCAAAAAGG GGGAAGCAAC GACCAATGGC CACTACCTAG AGGATTCGGC TGGAAGTTCA 
AGCGACAGCG AGTCGGTGCC ACCAGCCCCA CCACTAGTTC CTCGGCCAGT GGTGCCGGAT 
CGGGAGGAGG ATCCGTCAGC GGAGGGCCAG CAGGAGAATC TGGCGATGGA GGACCAGGAG 
GACTTGCACT CCAGCGTGGA GCGACTGCTT CCGAATCTGG CCACGAGTGC GATGGGGCCG 
CGTCCCTTAC TCACCACCCG TCTACTGCCC ACCACGGCTA TCGATCCTCA GCCGCCGGCA 
GTACTGGTGG CCTCATCCTG CGAGTATCTG CTCGATCAGC GCCCCTCGAG CGTACCAGGA 
CGCGATCTCG CCGGCGGGAG CATAGGCACC ACCACGATGA CATCCTCTCG GCCGACGCCT 
CTGTCTCCGG CGAAGGCAGC AGCGACACCG ATCGGGATGA ATGCGACGAT GACAACGAAG 
ACGACGACGA CCACGATGAT GACGCCGAGT CGAGCTCCCT CATCGACTAT TTCAACAATC 
AGCAGCGCGA GCGACACTAC CAGCTCCGGC GGCAGAGCCA GCGGCAGACC TCCGAGATTT 
GTACCGCCGC CACCGCCTCC GCGTCGCTTG CTCCTCACGC AGACCGATCT AATCGCTGGT 
CAGGCCAAAA CGGAGCTACC TCGAAAAAAA TCTGGCGCTA CTGCTGACAG TGGCTGCTCG 
GTGGCTGTCG GCGACGCCGC CAGCGCTTCG GACCCAACCG CTATCGGGTC AAGGACAATG 
CCATCAACAC CGAGTACCAG CAGCTTGGCG TCAGCGGTGG CCCCAAAACG ACCGTCATCA 
ACGATAATAG AGACCTGCCT GCTGGGGGCA CCCAGGCCCG CCTCAGCCAC TTCATCAGCA 
ACAGCCTCGA GAATGGCAAA GCCGATCCGC CAGAGTCCAC CCACCCAGCA CCAGTCGCAG 
CCGAGGGGTC AGAGCTGCCC GGCTGCAGTG CCAGCACCAT CGACGGCTGC GCCAGACGCA 
CCCACTGTCC CAATGCCCCA CGCTGGATGC GGCCTAAGCC CAAGGCTGGA AATGCGGCTG 
GCCCTCAACC ACGACATTCT CGGGGACGAG GACTTGATCT GCTACGAGCC TGGTCCCGAT 
CTAACGACTA TTCTGGGGCA CGACCTCTCC ACATTTCATC GTCTGACGGG TCGCGATTTA 
TTGAGTCGTT CAGCCACGAA CAGGGTGCAG CCAAAAGAGG CTGTCATATC GTACTCTCAA 
CAACGCAACT CAAAGATGGA CACGCCGACG GTGAACCGCC GGCCCCGCCC CCTCTCAGCG 
GGCGCCTTGG GCGGCTCCAC CAGCAACAGC AATCCGAACA ACAGTCGCAG CAGCAGCAGC 
CACGGCAACA GTCCGCTCGG CGGTGGTTTA ACCCGTGCCG GCGTCGAGCC AGTGGGCCAA 
CAGCAGCTGG TGGTTCAAGC AGGTGGTGGA TCTGCTGGTC GAGTAGGCGG CGGGAGCGGA 
AGCGCCGGCG GCAACAGCAC CGCCTGCAGC TCCAACAGAG GCGGGAGCAA ATTAGGCGAT 
CTGGAAATCT TAGCGAGACG CGAGAAGATA TACTCCATGT CTCAATCGAG GAGTGGCTGT 
CGAGTCAGGG AGACGACGTC CACGACCACT CTGATGGCCA TGGCTACGGA GATGCGTTCG 
GGGATGGATT CGGAGAAACG CGACTCTTCC TGGCCCAAAC GAGCAGCTGC AGCAGCCTCT 
CTGACGAGGA CGAGGAGTTC TACTTCGATG GACGGAGCGG GAGCCATCAG TACCACAACC 
ACAACCACCA CAACAATGAC TGAGGAGGCC TTCCTTGAGA CCGAAGTGGG CAGATCTAAG 
CGCTTGATAA ACTTTATAAA GCGTCGCAAC TCCGAGATAA CCGCCAGTAG CACCAGCAGC 
ACCCCCAACT TCTCCGACGG CGCCATTGGC GAGCAGCCCC AACCGAGCCA CAGCCAGAGC 
AGTCTTCTTC TCCAGAAGCT GCCGCCGGGT CAATCGCAGG CCCAGGACTC AGTGGAAATG 
GCCCAAATGT CGCCGCGCAA GGATAACGAC AAGCCATCGC TGAATCGCCG CCTCTGGAAG 
CAAATCACCA AACGCAGACG CACCAACTCC GTGTCCCAAA TAGTCGCAGG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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PsGEF cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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