Raf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Raf
Entrez Gene ID	31221
Full Name	Raf oncogene
Synonyms	11-29,C110,CG2845,D-RAF,D-Raf,D-raf,D-raf1,DRaf,DRaf1,Dmel\CG2845,Draf,Draf-1,Draf1,EG:BACH48C10.3,Phl,RAF,Raf/phl,Raf1,dRaf,draf,l(1)2Fe,l(1)G0475,l(1)ph,l(1)phl,l(1)pole hole,l(1)polehole,l(1)polehole/draf,l(1)raf,lincRNA.937,ph,ph1,phl,raf,raf-1,raf1,rafl
General protein information	Preferred Names Raf oncogene Names CG2845 gene product from transcript CG2845-RE CG2845-PA CG2845-PE Raf-PA Raf-PE l(1)pole hole pole hole pole-hole polehole raf-like-oncogene-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3A1-3A1\|1-0.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103397.3	NP_001096867.3	Raf oncogene, isoform E
NM_080308.4	NP_525047.1	Raf oncogene, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Braf	NP_647455.3
Rattus norvegicus (Norway rat)	Braf	XP_006236418.1
Gallus gallus (chicken)	BRAF	NP_990633.1
Caenorhabditis elegans (roundworm)	lin-45	NP_741430.3
Pan troglodytes (chimpanzee)	BRAF	XP_003951208.1
	LOC693554	XP_002803541.1
Bos taurus (cattle)	BRAF	XP_005205968.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004699	XP_318144.4
Canis lupus familiaris (dog)	BRAF	XP_005629607.1
Drosophila melanogaster (fruit fly)	phl	NP_525047.1
Xenopus tropicalis (tropical clawed frog)	braf	XP_004912520.1
Danio rerio (zebrafish)	braf	NP_991307.2
Homo sapiens (human)	BRAF	NP_004324.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Raf-activated intestinal stem cells and their progenies are very sensitive to apoptosis
Title: Wildtype adult stem cells, unlike tumor cells, are resistant to cellular damages in Drosophila.

Yorkie (Yki), a transcriptional coactivator in the Hippo pathway that regulates organ size, is required for Raf-induced cardiac hypertrophy in flies
Title: Cardiac hypertrophy induced by active Raf depends on Yorkie-mediated transcription.

move from the oocyte to the follicle cells in process that does not require endocytosis
Title: Germline and somatic vitelline proteins colocalize in aggregates in the follicular epithelium of Drosophila ovaries.

These results suggest that ERK is necessary, but not sufficient, for Raf-mediated cardiac hypertrophy.
Title: Raf-mediated cardiac hypertrophy in adult Drosophila.

RNAi-mediated inhibition of MAPK-signaling by knockdown of Ras85D, phl/D-Raf or Dsor1/MEK affects larval development, adult life span and hemolymph phosphate, suggesting that some in vivo effects involve activation of this signaling pathway by phosphate.
Title: Genetic determinants of phosphate response in Drosophila.

The following Raf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Raf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00597	NM_080308.4 Latest version!	Drosophila melanogaster Raf oncogene (Raf), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00597	NM_001103397.3 Latest version!	Drosophila melanogaster Raf oncogene (Raf), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Raf ( NM_001103397.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096867.3 Drosophila melanogaster Raf oncogene (Raf), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa00597
Clone ID Related Accession (Same CDS sequence)	NM_001103397.3 , NM_080308.4
Accession Version	NM_001103397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Raf oncogene, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103397.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCG AGTCCTCCAC CGAAGGCGAC AGCGATCTAT ACGATCCTTT GGCCGAGGAG 
CTGCACAACG TCCAGTTGGT CAAACATGTG ACCCGCGAGA ATATTGATGC CCTGAATGCC 
AAGTTTGCCA ACCTGCAGGA GCCACCAGCC ATGTACTTAA TAGAATACCA GGAGTTGACC 
TCCAAGCTCC ACGAACTGGA GGCCAAGGAG CAGGAACTAA TGGAGCGACT GAACTCGCAG 
GACCAGCAGG AGGACTCCTC CTTGGTCGAG CGGTTCAAGG AGCAGCCCCA CTATCAAAAT 
CAAACTCAAA TCCTGCAGCA ACAACGGCAA TTGGCGCGAG TGCACCACGG CAACGATCTA 
ACCGATAGCT TGGGCTCTCA GCCGGGCAGC CAATGTGGAA CTTTGACCCG TCAGCCCAAG 
ATCCTTTTGC GAGCCCACCT GCCCAATCAA CAGCGCACTT CAGTGGAGGT AATTTCGGGA 
GTACGACTAT GTGATGCCCT CATGAAGGCC CTGAAACTCC GGCAACTAAC GCCGGATATG 
TGCGAAGTAA GCACAACTCA TTCCGGAAGA CATATCATAC CCTGGCACAC GGATATCGGC 
ACTCTGCATG TGGAGGAGAT CTTTGTCAGG CTGCTGGATA AGTTTCCCAT TAGGACACAC 
ATCAAGCACC AGATCATACG GAAGACCTTC TTCTCGTTGG TATTCTGCGA GGGCTGTCGA 
AGGCTTCTGT TCACCGGGTT CTACTGTAGC CAGTGTAATT TTCGATTCCA TCAGAGGTGT 
GCCAATAGAG TGCCGATGCT GTGCCAGCCC TTTCCCATGG ATAGCTACTA TCAGCTACTG 
CTGGCCGAGA ATCCGGATAA TGGCGTTGGT TTCCCCGGCA GAGGCACTGC TGTCCGCTTC 
AATATGAGCA GCCGGAGTCG CAGTCGTCGT TGCAGCAGCA GTGGCAGCAG CAGCAGCTCG 
AAGCCACCAT CTTCATCCTC CGGCAATCAT CGACAGGGTC GTCCGCCGAG GATCAGCCAA 
GACGATCGAT CCAATTCCGC GCCAAATGTG TGCATCAACA ACATTCGATC GGTCACAAGC 
GAAGTGCAGC GCAGTTTGAT AATGCAGGCC AGACCTCCTT TGCCGCATCC GTGCACAGAT 
CACTCCAACT CCACGCAAGC GTCGCCCACG AGCACCTTGA AACACAATCG TCCCAGGGCC 
AGGTCCGCCG ATGAGAGCAA TAAAAATCTG CTTTTAAGAG ACGCCAAAAG TTCCGAGGAA 
AACTGGAATA TTCTGGCGGA GGAGATTTTA ATTGGGCCGC GCATCGGATC GGGTTCCTTT 
GGAACCGTTT ATCGCGCCCA TTGGCACGGT CCCGTGGCCG TAAAGACACT CAACGTGAAG 
ACACCGAGTC CCGCCCAGTT GCAGGCGTTT AAGAACGAGG TGGCCATGCT GAAAAAGACG 
CGCCACTGCA ATATCCTCCT CTTCATGGGC TGTGTATCCA AACCATCTCT AGCGATTGTG 
ACCCAGTGGT GCGAGGGCAG CAGTCTCTAC AAGCACGTCC ATGTCAGCGA AACCAAGTTT 
AAATTGAACA CGCTCATCGA TATCGGACGT CAGGTGGCCC AGGGCATGGA TTACCTGCAT 
GCCAAGAATA TCATTCATAG AGACCTCAAG TCAAACAACA TCTTTTTGCA CGAGGATCTT 
TCCGTGAAGA TAGGCGACTT CGGATTGGCC ACTGCGAAAA CTCGATGGTC GGGTGAAAAG 
CAAGCCAATC AACCCACGGG CAGTATTTTA TGGATGGCTC CAGAGGTGAT TCGCATGCAG 
GAGCTAAACC CCTACTCCTT CCAGTCGGAC GTTTATGCCT TTGGTATCGT GATGTACGAA 
CTGTTGGCGG AGTGCTTGCC CTACGGTCAT ATTAGCAACA AGGATCAGAT CCTGTTTATG 
GTGGGGCGAG GACTTCTGCG TCCGGACATG AGTCAAGTGC GCTCGGATGC GCCGCAGGCA 
TTGAAGCGCT TGGCCGAGGA TTGCATTAAG TATACCCCCA AGGATCGACC GCTCTTTAGG 
CCGCTGCTCA ATATGCTGGA GAACATGCTG CGCACTTTGC CCAAAATTCA TCGCAGTGCC 
AGTGAACCAA ACTTGACGCA ATCGCAGCTG CAGAACGATG AGTTTCTGTA TCTGCCCAGC 
CCGAAAACGC CGGTGAACTT CAACAACTTT CAGTTCTTCG GCAGCGCTGG GAATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096867.3
CDS	1589..3808
Translation	MSSESSTEGDSDLYDPLAEELHNVQLVKHVTRENIDALNAKFANLQEPPAMYLIEYQELTSKLHELEAKEQELMERLNSQDQQEDSSLVERFKEQPHYQNQTQILQQQRQLARVHHGNDLTDSLGSQPGSQCGTLTRQPKILLRAHLPNQQRTSVEVISGVRLCDALMKALKLRQLTPDMCEVSTTHSGRHIIPWHTDIGTLHVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFRFHQRCANRVPMLCQPFPMDSYYQLLLAENPDNGVGFPGRGTAVRFNMSSRSRSRRCSSSGSSSSSKPPSSSSGNHRQGRPPRISQDDRSNSAPNVCINNIRSVTSEVQRSLIMQARPPLPHPCTDHSNSTQASPTSTLKHNRPRARSADESNKNLLLRDAKSSEENWNILAEEILIGPRIGSGSFGTVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILFMVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASEPNLTQSQLQNDEFLYLPSPKTPVNFNNFQFFGSAGNI

Target ORF information:

RefSeq Version	NM_001103397.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Raf oncogene (Raf), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103397.3

ORF Insert Sequence:

ATGTCCAGCG AGTCCTCCAC CGAAGGCGAC AGCGATCTAT ACGATCCTTT GGCCGAGGAG 
CTGCACAACG TCCAGTTGGT CAAACATGTG ACCCGCGAGA ATATTGATGC CCTGAATGCC 
AAGTTTGCCA ACCTGCAGGA GCCACCAGCC ATGTACTTAA TAGAATACCA GGAGTTGACC 
TCCAAGCTCC ACGAACTGGA GGCCAAGGAG CAGGAACTAA TGGAGCGACT GAACTCGCAG 
GACCAGCAGG AGGACTCCTC CTTGGTCGAG CGGTTCAAGG AGCAGCCCCA CTATCAAAAT 
CAAACTCAAA TCCTGCAGCA ACAACGGCAA TTGGCGCGAG TGCACCACGG CAACGATCTA 
ACCGATAGCT TGGGCTCTCA GCCGGGCAGC CAATGTGGAA CTTTGACCCG TCAGCCCAAG 
ATCCTTTTGC GAGCCCACCT GCCCAATCAA CAGCGCACTT CAGTGGAGGT AATTTCGGGA 
GTACGACTAT GTGATGCCCT CATGAAGGCC CTGAAACTCC GGCAACTAAC GCCGGATATG 
TGCGAAGTAA GCACAACTCA TTCCGGAAGA CATATCATAC CCTGGCACAC GGATATCGGC 
ACTCTGCATG TGGAGGAGAT CTTTGTCAGG CTGCTGGATA AGTTTCCCAT TAGGACACAC 
ATCAAGCACC AGATCATACG GAAGACCTTC TTCTCGTTGG TATTCTGCGA GGGCTGTCGA 
AGGCTTCTGT TCACCGGGTT CTACTGTAGC CAGTGTAATT TTCGATTCCA TCAGAGGTGT 
GCCAATAGAG TGCCGATGCT GTGCCAGCCC TTTCCCATGG ATAGCTACTA TCAGCTACTG 
CTGGCCGAGA ATCCGGATAA TGGCGTTGGT TTCCCCGGCA GAGGCACTGC TGTCCGCTTC 
AATATGAGCA GCCGGAGTCG CAGTCGTCGT TGCAGCAGCA GTGGCAGCAG CAGCAGCTCG 
AAGCCACCAT CTTCATCCTC CGGCAATCAT CGACAGGGTC GTCCGCCGAG GATCAGCCAA 
GACGATCGAT CCAATTCCGC GCCAAATGTG TGCATCAACA ACATTCGATC GGTCACAAGC 
GAAGTGCAGC GCAGTTTGAT AATGCAGGCC AGACCTCCTT TGCCGCATCC GTGCACAGAT 
CACTCCAACT CCACGCAAGC GTCGCCCACG AGCACCTTGA AACACAATCG TCCCAGGGCC 
AGGTCCGCCG ATGAGAGCAA TAAAAATCTG CTTTTAAGAG ACGCCAAAAG TTCCGAGGAA 
AACTGGAATA TTCTGGCGGA GGAGATTTTA ATTGGGCCGC GCATCGGATC GGGTTCCTTT 
GGAACCGTTT ATCGCGCCCA TTGGCACGGT CCCGTGGCCG TAAAGACACT CAACGTGAAG 
ACACCGAGTC CCGCCCAGTT GCAGGCGTTT AAGAACGAGG TGGCCATGCT GAAAAAGACG 
CGCCACTGCA ATATCCTCCT CTTCATGGGC TGTGTATCCA AACCATCTCT AGCGATTGTG 
ACCCAGTGGT GCGAGGGCAG CAGTCTCTAC AAGCACGTCC ATGTCAGCGA AACCAAGTTT 
AAATTGAACA CGCTCATCGA TATCGGACGT CAGGTGGCCC AGGGCATGGA TTACCTGCAT 
GCCAAGAATA TCATTCATAG AGACCTCAAG TCAAACAACA TCTTTTTGCA CGAGGATCTT 
TCCGTGAAGA TAGGCGACTT CGGATTGGCC ACTGCGAAAA CTCGATGGTC GGGTGAAAAG 
CAAGCCAATC AACCCACGGG CAGTATTTTA TGGATGGCTC CAGAGGTGAT TCGCATGCAG 
GAGCTAAACC CCTACTCCTT CCAGTCGGAC GTTTATGCCT TTGGTATCGT GATGTACGAA 
CTGTTGGCGG AGTGCTTGCC CTACGGTCAT ATTAGCAACA AGGATCAGAT CCTGTTTATG 
GTGGGGCGAG GACTTCTGCG TCCGGACATG AGTCAAGTGC GCTCGGATGC GCCGCAGGCA 
TTGAAGCGCT TGGCCGAGGA TTGCATTAAG TATACCCCCA AGGATCGACC GCTCTTTAGG 
CCGCTGCTCA ATATGCTGGA GAACATGCTG CGCACTTTGC CCAAAATTCA TCGCAGTGCC 
AGTGAACCAA ACTTGACGCA ATCGCAGCTG CAGAACGATG AGTTTCTGTA TCTGCCCAGC 
CCGAAAACGC CGGTGAACTT CAACAACTTT CAGTTCTTCG GCAGCGCTGG GAATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Raf ( NM_080308.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525047.1 Drosophila melanogaster Raf oncogene (Raf), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00597
Clone ID Related Accession (Same CDS sequence)	NM_001103397.3 , NM_080308.4
Accession Version	NM_080308.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Raf oncogene, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080308.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCG AGTCCTCCAC CGAAGGCGAC AGCGATCTAT ACGATCCTTT GGCCGAGGAG 
CTGCACAACG TCCAGTTGGT CAAACATGTG ACCCGCGAGA ATATTGATGC CCTGAATGCC 
AAGTTTGCCA ACCTGCAGGA GCCACCAGCC ATGTACTTAA TAGAATACCA GGAGTTGACC 
TCCAAGCTCC ACGAACTGGA GGCCAAGGAG CAGGAACTAA TGGAGCGACT GAACTCGCAG 
GACCAGCAGG AGGACTCCTC CTTGGTCGAG CGGTTCAAGG AGCAGCCCCA CTATCAAAAT 
CAAACTCAAA TCCTGCAGCA ACAACGGCAA TTGGCGCGAG TGCACCACGG CAACGATCTA 
ACCGATAGCT TGGGCTCTCA GCCGGGCAGC CAATGTGGAA CTTTGACCCG TCAGCCCAAG 
ATCCTTTTGC GAGCCCACCT GCCCAATCAA CAGCGCACTT CAGTGGAGGT AATTTCGGGA 
GTACGACTAT GTGATGCCCT CATGAAGGCC CTGAAACTCC GGCAACTAAC GCCGGATATG 
TGCGAAGTAA GCACAACTCA TTCCGGAAGA CATATCATAC CCTGGCACAC GGATATCGGC 
ACTCTGCATG TGGAGGAGAT CTTTGTCAGG CTGCTGGATA AGTTTCCCAT TAGGACACAC 
ATCAAGCACC AGATCATACG GAAGACCTTC TTCTCGTTGG TATTCTGCGA GGGCTGTCGA 
AGGCTTCTGT TCACCGGGTT CTACTGTAGC CAGTGTAATT TTCGATTCCA TCAGAGGTGT 
GCCAATAGAG TGCCGATGCT GTGCCAGCCC TTTCCCATGG ATAGCTACTA TCAGCTACTG 
CTGGCCGAGA ATCCGGATAA TGGCGTTGGT TTCCCCGGCA GAGGCACTGC TGTCCGCTTC 
AATATGAGCA GCCGGAGTCG CAGTCGTCGT TGCAGCAGCA GTGGCAGCAG CAGCAGCTCG 
AAGCCACCAT CTTCATCCTC CGGCAATCAT CGACAGGGTC GTCCGCCGAG GATCAGCCAA 
GACGATCGAT CCAATTCCGC GCCAAATGTG TGCATCAACA ACATTCGATC GGTCACAAGC 
GAAGTGCAGC GCAGTTTGAT AATGCAGGCC AGACCTCCTT TGCCGCATCC GTGCACAGAT 
CACTCCAACT CCACGCAAGC GTCGCCCACG AGCACCTTGA AACACAATCG TCCCAGGGCC 
AGGTCCGCCG ATGAGAGCAA TAAAAATCTG CTTTTAAGAG ACGCCAAAAG TTCCGAGGAA 
AACTGGAATA TTCTGGCGGA GGAGATTTTA ATTGGGCCGC GCATCGGATC GGGTTCCTTT 
GGAACCGTTT ATCGCGCCCA TTGGCACGGT CCCGTGGCCG TAAAGACACT CAACGTGAAG 
ACACCGAGTC CCGCCCAGTT GCAGGCGTTT AAGAACGAGG TGGCCATGCT GAAAAAGACG 
CGCCACTGCA ATATCCTCCT CTTCATGGGC TGTGTATCCA AACCATCTCT AGCGATTGTG 
ACCCAGTGGT GCGAGGGCAG CAGTCTCTAC AAGCACGTCC ATGTCAGCGA AACCAAGTTT 
AAATTGAACA CGCTCATCGA TATCGGACGT CAGGTGGCCC AGGGCATGGA TTACCTGCAT 
GCCAAGAATA TCATTCATAG AGACCTCAAG TCAAACAACA TCTTTTTGCA CGAGGATCTT 
TCCGTGAAGA TAGGCGACTT CGGATTGGCC ACTGCGAAAA CTCGATGGTC GGGTGAAAAG 
CAAGCCAATC AACCCACGGG CAGTATTTTA TGGATGGCTC CAGAGGTGAT TCGCATGCAG 
GAGCTAAACC CCTACTCCTT CCAGTCGGAC GTTTATGCCT TTGGTATCGT GATGTACGAA 
CTGTTGGCGG AGTGCTTGCC CTACGGTCAT ATTAGCAACA AGGATCAGAT CCTGTTTATG 
GTGGGGCGAG GACTTCTGCG TCCGGACATG AGTCAAGTGC GCTCGGATGC GCCGCAGGCA 
TTGAAGCGCT TGGCCGAGGA TTGCATTAAG TATACCCCCA AGGATCGACC GCTCTTTAGG 
CCGCTGCTCA ATATGCTGGA GAACATGCTG CGCACTTTGC CCAAAATTCA TCGCAGTGCC 
AGTGAACCAA ACTTGACGCA ATCGCAGCTG CAGAACGATG AGTTTCTGTA TCTGCCCAGC 
CCGAAAACGC CGGTGAACTT CAACAACTTT CAGTTCTTCG GCAGCGCTGG GAATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525047.1
CDS	589..2808
Translation	MSSESSTEGDSDLYDPLAEELHNVQLVKHVTRENIDALNAKFANLQEPPAMYLIEYQELTSKLHELEAKEQELMERLNSQDQQEDSSLVERFKEQPHYQNQTQILQQQRQLARVHHGNDLTDSLGSQPGSQCGTLTRQPKILLRAHLPNQQRTSVEVISGVRLCDALMKALKLRQLTPDMCEVSTTHSGRHIIPWHTDIGTLHVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFRFHQRCANRVPMLCQPFPMDSYYQLLLAENPDNGVGFPGRGTAVRFNMSSRSRSRRCSSSGSSSSSKPPSSSSGNHRQGRPPRISQDDRSNSAPNVCINNIRSVTSEVQRSLIMQARPPLPHPCTDHSNSTQASPTSTLKHNRPRARSADESNKNLLLRDAKSSEENWNILAEEILIGPRIGSGSFGTVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILFMVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASEPNLTQSQLQNDEFLYLPSPKTPVNFNNFQFFGSAGNI

Target ORF information:

RefSeq Version	NM_080308.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Raf oncogene (Raf), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080308.4

ORF Insert Sequence:

ATGTCCAGCG AGTCCTCCAC CGAAGGCGAC AGCGATCTAT ACGATCCTTT GGCCGAGGAG 
CTGCACAACG TCCAGTTGGT CAAACATGTG ACCCGCGAGA ATATTGATGC CCTGAATGCC 
AAGTTTGCCA ACCTGCAGGA GCCACCAGCC ATGTACTTAA TAGAATACCA GGAGTTGACC 
TCCAAGCTCC ACGAACTGGA GGCCAAGGAG CAGGAACTAA TGGAGCGACT GAACTCGCAG 
GACCAGCAGG AGGACTCCTC CTTGGTCGAG CGGTTCAAGG AGCAGCCCCA CTATCAAAAT 
CAAACTCAAA TCCTGCAGCA ACAACGGCAA TTGGCGCGAG TGCACCACGG CAACGATCTA 
ACCGATAGCT TGGGCTCTCA GCCGGGCAGC CAATGTGGAA CTTTGACCCG TCAGCCCAAG 
ATCCTTTTGC GAGCCCACCT GCCCAATCAA CAGCGCACTT CAGTGGAGGT AATTTCGGGA 
GTACGACTAT GTGATGCCCT CATGAAGGCC CTGAAACTCC GGCAACTAAC GCCGGATATG 
TGCGAAGTAA GCACAACTCA TTCCGGAAGA CATATCATAC CCTGGCACAC GGATATCGGC 
ACTCTGCATG TGGAGGAGAT CTTTGTCAGG CTGCTGGATA AGTTTCCCAT TAGGACACAC 
ATCAAGCACC AGATCATACG GAAGACCTTC TTCTCGTTGG TATTCTGCGA GGGCTGTCGA 
AGGCTTCTGT TCACCGGGTT CTACTGTAGC CAGTGTAATT TTCGATTCCA TCAGAGGTGT 
GCCAATAGAG TGCCGATGCT GTGCCAGCCC TTTCCCATGG ATAGCTACTA TCAGCTACTG 
CTGGCCGAGA ATCCGGATAA TGGCGTTGGT TTCCCCGGCA GAGGCACTGC TGTCCGCTTC 
AATATGAGCA GCCGGAGTCG CAGTCGTCGT TGCAGCAGCA GTGGCAGCAG CAGCAGCTCG 
AAGCCACCAT CTTCATCCTC CGGCAATCAT CGACAGGGTC GTCCGCCGAG GATCAGCCAA 
GACGATCGAT CCAATTCCGC GCCAAATGTG TGCATCAACA ACATTCGATC GGTCACAAGC 
GAAGTGCAGC GCAGTTTGAT AATGCAGGCC AGACCTCCTT TGCCGCATCC GTGCACAGAT 
CACTCCAACT CCACGCAAGC GTCGCCCACG AGCACCTTGA AACACAATCG TCCCAGGGCC 
AGGTCCGCCG ATGAGAGCAA TAAAAATCTG CTTTTAAGAG ACGCCAAAAG TTCCGAGGAA 
AACTGGAATA TTCTGGCGGA GGAGATTTTA ATTGGGCCGC GCATCGGATC GGGTTCCTTT 
GGAACCGTTT ATCGCGCCCA TTGGCACGGT CCCGTGGCCG TAAAGACACT CAACGTGAAG 
ACACCGAGTC CCGCCCAGTT GCAGGCGTTT AAGAACGAGG TGGCCATGCT GAAAAAGACG 
CGCCACTGCA ATATCCTCCT CTTCATGGGC TGTGTATCCA AACCATCTCT AGCGATTGTG 
ACCCAGTGGT GCGAGGGCAG CAGTCTCTAC AAGCACGTCC ATGTCAGCGA AACCAAGTTT 
AAATTGAACA CGCTCATCGA TATCGGACGT CAGGTGGCCC AGGGCATGGA TTACCTGCAT 
GCCAAGAATA TCATTCATAG AGACCTCAAG TCAAACAACA TCTTTTTGCA CGAGGATCTT 
TCCGTGAAGA TAGGCGACTT CGGATTGGCC ACTGCGAAAA CTCGATGGTC GGGTGAAAAG 
CAAGCCAATC AACCCACGGG CAGTATTTTA TGGATGGCTC CAGAGGTGAT TCGCATGCAG 
GAGCTAAACC CCTACTCCTT CCAGTCGGAC GTTTATGCCT TTGGTATCGT GATGTACGAA 
CTGTTGGCGG AGTGCTTGCC CTACGGTCAT ATTAGCAACA AGGATCAGAT CCTGTTTATG 
GTGGGGCGAG GACTTCTGCG TCCGGACATG AGTCAAGTGC GCTCGGATGC GCCGCAGGCA 
TTGAAGCGCT TGGCCGAGGA TTGCATTAAG TATACCCCCA AGGATCGACC GCTCTTTAGG 
CCGCTGCTCA ATATGCTGGA GAACATGCTG CGCACTTTGC CCAAAATTCA TCGCAGTGCC 
AGTGAACCAA ACTTGACGCA ATCGCAGCTG CAGAACGATG AGTTTCTGTA TCTGCCCAGC 
CCGAAAACGC CGGTGAACTT CAACAACTTT CAGTTCTTCG GCAGCGCTGG GAATATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Raf cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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