crn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	crn
Entrez Gene ID	31208
Full Name	crooked neck
Synonyms	62D9.a,CG18842,CG3193,Crn,Dmel\CG3193,EG:30B8.1,N10,N5,RC63,l(1)2,l(1)2Fa,l(1)CRN,l(1)N7,l(1)crn,l30,yok
General protein information	Preferred Names crooked neck Names CG3193 gene product from transcript CG3193-RA CG3193-PA crn-PA yolky
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2F2-2F2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057770.5	NP_477118.1	crooked neck

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CRNKL1	NP_001193451.1
Rattus norvegicus (Norway rat)	LOC102548514	XP_006235227.1
Arabidopsis thaliana (thale cress)	AT3G13210	NP_187927.1
Oryza sativa (rice)	Os06g0523800	NP_001174825.1
Homo sapiens (human)	CRNKL1	NP_057736.4
Pan troglodytes (chimpanzee)	CRNKL1	XP_514541.2
Gallus gallus (chicken)	CRNKL1	XP_419315.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001879	XP_321188.4
Neurospora crassa	NCU02717	XP_965102.1
Arabidopsis thaliana (thale cress)	AT5G45990	NP_199411.1
Danio rerio (zebrafish)	crnkl1	NP_957240.1
Caenorhabditis elegans (roundworm)	M03F8.3	NP_001122979.1
Schizosaccharomyces pombe (fission yeast)	cwf4	NP_596573.1
Magnaporthe oryzae (rice blast fungus)	MGG_05720	XP_003710622.1
Oryza sativa (rice)	Os05g0289400	NP_001055097.1
Xenopus tropicalis (tropical clawed frog)	crnkl1	NP_001016895.1
Arabidopsis thaliana (thale cress)	AT5G41770	NP_198992.2
	LOC699931	XP_002808010.1
Canis lupus familiaris (dog)	CRNKL1	XP_005634771.1
Mus musculus (house mouse)	Crnkl1	NP_080096.1
Rattus norvegicus (Norway rat)	Crnkl1	NP_446249.1
Drosophila melanogaster (fruit fly)	crn	NP_477118.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Through an RNAi-based candidate gene screen, the study identifies the spliceosome factor Crooked neck (Crn) as a regulator of Su(fu) level.
Title: Hedgehog signaling downregulates suppressor of fused through the HIB/SPOP-Crn axis in Drosophila.

Data show that Crn does not have a cell cycle function in the embryo, but is found in a 900 kDa complex together with snRNPs, suggesting that Crn participates in the assembly of the splicing machinery in vivo.
Title: Drosophila crooked-neck protein co-fractionates in a multiprotein complex with splicing factors.

The following crn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00583	NM_057770.5 Latest version!	Drosophila melanogaster crooked neck (crn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crn ( NM_057770.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477118.1 Drosophila melanogaster crooked neck (crn), mRNA.

Price & Availability↑

CloneID	OFa00583
Clone ID Related Accession (Same CDS sequence)	NM_057770.5
Accession Version	NM_057770.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crooked neck
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057770.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCGGC CACAGAAGAT GCCCAAGGTG GCCAAGGTCA AAAACAAAGC GCCGGCGGAG 
GTGCAAATTA CGGCGGAGCA GTTGCTCCGT GAGGCCAAGG AGCGAGATCT GGAGATTCTG 
CCGCCGCCGC CAAAGCAGAA GATCTCCGAT CCCGCCGAGT TGGCAGACTA TCAGCAGCGG 
AAGCGAAAGA CCTTCGAGGA CAATCTCCGC AAGAACCGCA TGGTAGTCAG CCATTGGATC 
AAATATGCTC AGTGGGAGGA GCAGCAGCAG GAGATCCAGC GGGCGCGCTC TATCTGGGAG 
CGGGCGTTGG ACAATGAGCA CCGGAACGTG ACCCTCTGGC TGAAATACGC CGAGATGGAG 
ATGAAGAACA AGCAGGTGAA TCATGCTCGC AATCTGTGGG ATCGGGCAGT GACCATCATG 
CCGCGAGTCA ACCAGTTCTG GTACAAGTAC ACCTACATGG AGGAGATGCT GGAGAATGTG 
GCAGGGGCGC GACAGGTGTT CGAGCGCTGG ATGGAGTGGC AACCGGAGGA GCAGGCCTGG 
CAGACCTACG TCAACTTCGA GCTGCGCTAC AAGGAGATCG ACCGGGCACG GGAGATCTAC 
GAGCGATTTG TCTATGTGCA CCCGGACGTG AAGAACTGGA TAAAGTTCGC CCGCTTTGAG 
GAGTCTCATG GGTTTATCCA CGGCTCTCGG CGGGTATTTG AGCGCGCTGT GGAGTTCTTT 
GGTGACGACT ACATCGAGGA GCGCCTGTTC ATCGCCTTTG CACGTTTCGA GGAGGGTCAA 
AAGGAGCACG ACAGGGCGCG CATCATCTAC AAGTACGCTT TAGATCACCT ACCTAAAGAT 
CGCACACAGG AGCTGTTCAA GGCCTATACG AAACACGAGA AAAAGTACGG CGACAGAGCG 
GGTATTGAGG ATGTGATTGT TTCCAAGCGC AAGTACCAGT ACGAGCAGGA GGTGGCCGCC 
AATCCCACCA ACTACGATGC CTGGTTCGAC TATTTGCGCT TGATTGAGGC TGAGGGCGAT 
CGGGATCAGA TCCGTGAGAC GTACGAACGG GCCATTTCCA ACGTGCCGCC TGCCAATGAA 
AAGAACTTCT GGCGACGCTA CATTTACCTT TGGATCAATT ATGCTTTGTA CGAGGAGCTT 
GAAGCGGAGG ACGCGGAACG CACGCGGCAG ATCTACAAGA CCTGTCTGGA GCTAATACCT 
CACAAGCAGT TCACCTTCAG CAAGCTTTGG CTGCTGTACG CCCAATTCGA GATCCGCTGC 
AAGGAGCTGC AGCGAGCACG CAAAGCTCTG GGCTTGGCCA TCGGCATGTG TCCCAGAGAT 
AAGCTCTTCA GAGGCTATAT TGATCTGGAG ATTCAGTTGC GTGAGTTCGA GCGCTGCCGC 
ATGCTGTACG AGAAGTTCCT GGAGTTTGGA CCGGAGAATT GTGTCACTTG GATGAAGTTT 
GCCGAGCTGG AGAATCTGTT GGGCGACACG GACCGGGCGA GAGCCATTTT CGAGCTGGCG 
GTGCAGCAGC CGCGCCTGGA CATGCCAGAG CTGCTGTGGA AGGCGTACAT CGACTTCGAG 
GTAGCCCTAG GCGAAACAGA GCTAGCCAGA CAGCTTTACG AGCGACTCCT TGAGCGGACG 
CAGCATGTGA AAGTGTGGAT GTCGTTTGCC AAGTTCGAGA TGGGCCTCAG CCACGGCGAC 
AGCGGGCCAG ATGCGGAGCT GAACGTGCAG CTGGCAAGGA GAATCTATGA GCGAGCCAAC 
GAGATGTTGC GTCAGCTGGG AGACAAGGAG TCGCGTGTTC TGTTGCTAGA GGCATGGCGC 
GACTTTGAAC GCGACGCCTC CGATTCGCAG GAGATGCAAA AGGTAATGGA CAAGATGCCG 
CGGCGCATCA AGAAGCGGCA AAAGATCGTC TCGGACAATG GCGTCGAGGA AGGTTGGGAG 
GAGGTATTCG ACTACATCTT TCCCGAGGAC GAAATGGCTC GACCCAATCT CAAGTTGCTG 
GCTGCAGCTA AGATGTGGAA GACACAAAAA GACAACACGG TGGACGACCC GCCCGCTACA 
GCTATTGCAT CTGAGCCAGA ACCTGCAGCT GATGCTGCCC CAGCGGATAC GACGGATAGC 
GGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477118.1
CDS	121..2229
Translation	MERPQKMPKVAKVKNKAPAEVQITAEQLLREAKERDLEILPPPPKQKISDPAELADYQQRKRKTFEDNLRKNRMVVSHWIKYAQWEEQQQEIQRARSIWERALDNEHRNVTLWLKYAEMEMKNKQVNHARNLWDRAVTIMPRVNQFWYKYTYMEEMLENVAGARQVFERWMEWQPEEQAWQTYVNFELRYKEIDRAREIYERFVYVHPDVKNWIKFARFEESHGFIHGSRRVFERAVEFFGDDYIEERLFIAFARFEEGQKEHDRARIIYKYALDHLPKDRTQELFKAYTKHEKKYGDRAGIEDVIVSKRKYQYEQEVAANPTNYDAWFDYLRLIEAEGDRDQIRETYERAISNVPPANEKNFWRRYIYLWINYALYEELEAEDAERTRQIYKTCLELIPHKQFTFSKLWLLYAQFEIRCKELQRARKALGLAIGMCPRDKLFRGYIDLEIQLREFERCRMLYEKFLEFGPENCVTWMKFAELENLLGDTDRARAIFELAVQQPRLDMPELLWKAYIDFEVALGETELARQLYERLLERTQHVKVWMSFAKFEMGLSHGDSGPDAELNVQLARRIYERANEMLRQLGDKESRVLLLEAWRDFERDASDSQEMQKVMDKMPRRIKKRQKIVSDNGVEEGWEEVFDYIFPEDEMARPNLKLLAAAKMWKTQKDNTVDDPPATAIASEPEPAADAAPADTTDSGD

Target ORF information:

RefSeq Version	NM_057770.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crooked neck (crn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057770.5

ORF Insert Sequence:

ATGGAGCGGC CACAGAAGAT GCCCAAGGTG GCCAAGGTCA AAAACAAAGC GCCGGCGGAG 
GTGCAAATTA CGGCGGAGCA GTTGCTCCGT GAGGCCAAGG AGCGAGATCT GGAGATTCTG 
CCGCCGCCGC CAAAGCAGAA GATCTCCGAT CCCGCCGAGT TGGCAGACTA TCAGCAGCGG 
AAGCGAAAGA CCTTCGAGGA CAATCTCCGC AAGAACCGCA TGGTAGTCAG CCATTGGATC 
AAATATGCTC AGTGGGAGGA GCAGCAGCAG GAGATCCAGC GGGCGCGCTC TATCTGGGAG 
CGGGCGTTGG ACAATGAGCA CCGGAACGTG ACCCTCTGGC TGAAATACGC CGAGATGGAG 
ATGAAGAACA AGCAGGTGAA TCATGCTCGC AATCTGTGGG ATCGGGCAGT GACCATCATG 
CCGCGAGTCA ACCAGTTCTG GTACAAGTAC ACCTACATGG AGGAGATGCT GGAGAATGTG 
GCAGGGGCGC GACAGGTGTT CGAGCGCTGG ATGGAGTGGC AACCGGAGGA GCAGGCCTGG 
CAGACCTACG TCAACTTCGA GCTGCGCTAC AAGGAGATCG ACCGGGCACG GGAGATCTAC 
GAGCGATTTG TCTATGTGCA CCCGGACGTG AAGAACTGGA TAAAGTTCGC CCGCTTTGAG 
GAGTCTCATG GGTTTATCCA CGGCTCTCGG CGGGTATTTG AGCGCGCTGT GGAGTTCTTT 
GGTGACGACT ACATCGAGGA GCGCCTGTTC ATCGCCTTTG CACGTTTCGA GGAGGGTCAA 
AAGGAGCACG ACAGGGCGCG CATCATCTAC AAGTACGCTT TAGATCACCT ACCTAAAGAT 
CGCACACAGG AGCTGTTCAA GGCCTATACG AAACACGAGA AAAAGTACGG CGACAGAGCG 
GGTATTGAGG ATGTGATTGT TTCCAAGCGC AAGTACCAGT ACGAGCAGGA GGTGGCCGCC 
AATCCCACCA ACTACGATGC CTGGTTCGAC TATTTGCGCT TGATTGAGGC TGAGGGCGAT 
CGGGATCAGA TCCGTGAGAC GTACGAACGG GCCATTTCCA ACGTGCCGCC TGCCAATGAA 
AAGAACTTCT GGCGACGCTA CATTTACCTT TGGATCAATT ATGCTTTGTA CGAGGAGCTT 
GAAGCGGAGG ACGCGGAACG CACGCGGCAG ATCTACAAGA CCTGTCTGGA GCTAATACCT 
CACAAGCAGT TCACCTTCAG CAAGCTTTGG CTGCTGTACG CCCAATTCGA GATCCGCTGC 
AAGGAGCTGC AGCGAGCACG CAAAGCTCTG GGCTTGGCCA TCGGCATGTG TCCCAGAGAT 
AAGCTCTTCA GAGGCTATAT TGATCTGGAG ATTCAGTTGC GTGAGTTCGA GCGCTGCCGC 
ATGCTGTACG AGAAGTTCCT GGAGTTTGGA CCGGAGAATT GTGTCACTTG GATGAAGTTT 
GCCGAGCTGG AGAATCTGTT GGGCGACACG GACCGGGCGA GAGCCATTTT CGAGCTGGCG 
GTGCAGCAGC CGCGCCTGGA CATGCCAGAG CTGCTGTGGA AGGCGTACAT CGACTTCGAG 
GTAGCCCTAG GCGAAACAGA GCTAGCCAGA CAGCTTTACG AGCGACTCCT TGAGCGGACG 
CAGCATGTGA AAGTGTGGAT GTCGTTTGCC AAGTTCGAGA TGGGCCTCAG CCACGGCGAC 
AGCGGGCCAG ATGCGGAGCT GAACGTGCAG CTGGCAAGGA GAATCTATGA GCGAGCCAAC 
GAGATGTTGC GTCAGCTGGG AGACAAGGAG TCGCGTGTTC TGTTGCTAGA GGCATGGCGC 
GACTTTGAAC GCGACGCCTC CGATTCGCAG GAGATGCAAA AGGTAATGGA CAAGATGCCG 
CGGCGCATCA AGAAGCGGCA AAAGATCGTC TCGGACAATG GCGTCGAGGA AGGTTGGGAG 
GAGGTATTCG ACTACATCTT TCCCGAGGAC GAAATGGCTC GACCCAATCT CAAGTTGCTG 
GCTGCAGCTA AGATGTGGAA GACACAAAAA GACAACACGG TGGACGACCC GCCCGCTACA 
GCTATTGCAT CTGAGCCAGA ACCTGCAGCT GATGCTGCCC CAGCGGATAC GACGGATAGC 
GGTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

crn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報