inc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	inc
Entrez Gene ID	31110
Full Name	insomniac
Synonyms	CG14783,CG14784,CG32810,Dmel\CG32810,EG:196F3.2,ins
General protein information	Preferred Names insomniac Names CG32810 gene product from transcript CG32810-RD CG32810-PB CG32810-PC CG32810-PD inc-PB inc-PC inc-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2B1-2B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_130570.3	NP_569926.2	insomniac, isoform B
NM_001297857.1	NP_001284786.1	insomniac, isoform D
NM_001297858.1	NP_001284787.1	insomniac, isoform C

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000083	XP_311068.3
Xenopus tropicalis (tropical clawed frog)	kctd5	NP_001017294.1
Pan troglodytes (chimpanzee)	LOC453840	XP_003314978.2
Mus musculus (house mouse)	Kctd5	NP_081284.2
Gallus gallus (chicken)	KCTD5	XP_004945339.1
Canis lupus familiaris (dog)	KCTD5	XP_547178.2
Danio rerio (zebrafish)	kctd5a	NP_996932.1
Homo sapiens (human)	KCTD5	NP_061865.1
Bos taurus (cattle)	KCTD5	NP_001096811.1
Rattus norvegicus (Norway rat)	Kctd5	NP_001099238.1
Drosophila melanogaster (fruit fly)	inc	NP_569926.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

inc mutants exhibit defects in synaptic structure and physiology, indicating that Inc is essential for both sleep and synaptic function. Inc and its mouse orthologs exhibit striking biochemical and functional interchangeability within Cul3 complexes.
Title: Conserved properties of Drosophila Insomniac link sleep regulation and synaptic function.

Data show that insomniac protein (INC) recognize its potential partners Cul3 and dGRASP.
Title: Proteins involved in sleep homeostasis: Biophysical characterization of INC and its partners.

these results establish inc and Cul3 as important new players in setting the sleep homeostat and a dopaminergic arousal pathway in Drosophila.
Title: Cul3 and the BTB adaptor insomniac are key regulators of sleep homeostasis and a dopamine arousal pathway in Drosophila.

The following inc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the inc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00488	NM_001297858.1 Latest version!	Drosophila melanogaster insomniac (inc), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00488	NM_130570.3 Latest version!	Drosophila melanogaster insomniac (inc), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa00488	NM_001297857.1 Latest version!	Drosophila melanogaster insomniac (inc), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

inc ( NM_130570.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569926.2 Drosophila melanogaster insomniac (inc), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00488
Clone ID Related Accession (Same CDS sequence)	NM_130570.3 , NM_001297857.1 , NM_001297858.1
Accession Version	NM_130570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insomniac, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130570.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACGG TGTTCATAAA CTCGCGCAAG AGCCCCAACG TGCTGAAAAA ACAGGGCACC 
GACCAGTGGG TCAAGCTGAA CGTAGGCGGT ACCTACTTCC TCACCACAAA GACGACGCTC 
TCCCGTGACC CAAATTCGTT CCTCTCCCGT CTGATTCAGG AGGACTGCGA CTTGATATCA 
GATCGGGACG AGACAGGAGC CTACCTGATC GACAGAGACC CCAAATACTT TGCACCCGTG 
CTCAATTACC TGCGCCACGG CAAGCTTGTG CTCGATGGCG TCTCAGAGGA AGGCGTCCTG 
GAGGAGGCTG AGTTCTACAA CGTGACGCAG CTGATAGCGC TGCTGAAGGA GTGCATCCTG 
CACAGGGATC AGCGACCCCA AACGGACAAG AAGCGCGTTT ATCGTGTGCT GCAGTGCCGC 
GAGCAGGAAC TAACCCAGAT GATCTCAACG CTGTCGGATG GCTGGAGGTT CGAGCAGCTG 
ATCAGCATGC AGTACACGAA CTACGGGCCC TTCGAAAACA ATGAGTTCCT GTGCGTGGTA 
TCCAAAGAGT GTGGCACAAC GGCCGGCCGG GAGCTGGAGC TCAACGACCG GGCCAAGGTC 
CTGCAGCAGA AGGGATCGCG AATTCTTGGA ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569926.2
CDS	394..1029
Translation	MSTVFINSRKSPNVLKKQGTDQWVKLNVGGTYFLTTKTTLSRDPNSFLSRLIQEDCDLISDRDETGAYLIDRDPKYFAPVLNYLRHGKLVLDGVSEEGVLEEAEFYNVTQLIALLKECILHRDQRPQTDKKRVYRVLQCREQELTQMISTLSDGWRFEQLISMQYTNYGPFENNEFLCVVSKECGTTAGRELELNDRAKVLQQKGSRILGI

Target ORF information:

RefSeq Version	NM_130570.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insomniac (inc), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130570.3

ORF Insert Sequence:

ATGAGCACGG TGTTCATAAA CTCGCGCAAG AGCCCCAACG TGCTGAAAAA ACAGGGCACC 
GACCAGTGGG TCAAGCTGAA CGTAGGCGGT ACCTACTTCC TCACCACAAA GACGACGCTC 
TCCCGTGACC CAAATTCGTT CCTCTCCCGT CTGATTCAGG AGGACTGCGA CTTGATATCA 
GATCGGGACG AGACAGGAGC CTACCTGATC GACAGAGACC CCAAATACTT TGCACCCGTG 
CTCAATTACC TGCGCCACGG CAAGCTTGTG CTCGATGGCG TCTCAGAGGA AGGCGTCCTG 
GAGGAGGCTG AGTTCTACAA CGTGACGCAG CTGATAGCGC TGCTGAAGGA GTGCATCCTG 
CACAGGGATC AGCGACCCCA AACGGACAAG AAGCGCGTTT ATCGTGTGCT GCAGTGCCGC 
GAGCAGGAAC TAACCCAGAT GATCTCAACG CTGTCGGATG GCTGGAGGTT CGAGCAGCTG 
ATCAGCATGC AGTACACGAA CTACGGGCCC TTCGAAAACA ATGAGTTCCT GTGCGTGGTA 
TCCAAAGAGT GTGGCACAAC GGCCGGCCGG GAGCTGGAGC TCAACGACCG GGCCAAGGTC 
CTGCAGCAGA AGGGATCGCG AATTCTTGGA ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

inc ( NM_001297857.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284786.1 Drosophila melanogaster insomniac (inc), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa00488
Clone ID Related Accession (Same CDS sequence)	NM_130570.3 , NM_001297857.1 , NM_001297858.1
Accession Version	NM_001297857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insomniac, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACGG TGTTCATAAA CTCGCGCAAG AGCCCCAACG TGCTGAAAAA ACAGGGCACC 
GACCAGTGGG TCAAGCTGAA CGTAGGCGGT ACCTACTTCC TCACCACAAA GACGACGCTC 
TCCCGTGACC CAAATTCGTT CCTCTCCCGT CTGATTCAGG AGGACTGCGA CTTGATATCA 
GATCGGGACG AGACAGGAGC CTACCTGATC GACAGAGACC CCAAATACTT TGCACCCGTG 
CTCAATTACC TGCGCCACGG CAAGCTTGTG CTCGATGGCG TCTCAGAGGA AGGCGTCCTG 
GAGGAGGCTG AGTTCTACAA CGTGACGCAG CTGATAGCGC TGCTGAAGGA GTGCATCCTG 
CACAGGGATC AGCGACCCCA AACGGACAAG AAGCGCGTTT ATCGTGTGCT GCAGTGCCGC 
GAGCAGGAAC TAACCCAGAT GATCTCAACG CTGTCGGATG GCTGGAGGTT CGAGCAGCTG 
ATCAGCATGC AGTACACGAA CTACGGGCCC TTCGAAAACA ATGAGTTCCT GTGCGTGGTA 
TCCAAAGAGT GTGGCACAAC GGCCGGCCGG GAGCTGGAGC TCAACGACCG GGCCAAGGTC 
CTGCAGCAGA AGGGATCGCG AATTCTTGGA ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284786.1
CDS	1826..2461
Translation	MSTVFINSRKSPNVLKKQGTDQWVKLNVGGTYFLTTKTTLSRDPNSFLSRLIQEDCDLISDRDETGAYLIDRDPKYFAPVLNYLRHGKLVLDGVSEEGVLEEAEFYNVTQLIALLKECILHRDQRPQTDKKRVYRVLQCREQELTQMISTLSDGWRFEQLISMQYTNYGPFENNEFLCVVSKECGTTAGRELELNDRAKVLQQKGSRILGI

Target ORF information:

RefSeq Version	NM_001297857.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insomniac (inc), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297857.1

ORF Insert Sequence:

ATGAGCACGG TGTTCATAAA CTCGCGCAAG AGCCCCAACG TGCTGAAAAA ACAGGGCACC 
GACCAGTGGG TCAAGCTGAA CGTAGGCGGT ACCTACTTCC TCACCACAAA GACGACGCTC 
TCCCGTGACC CAAATTCGTT CCTCTCCCGT CTGATTCAGG AGGACTGCGA CTTGATATCA 
GATCGGGACG AGACAGGAGC CTACCTGATC GACAGAGACC CCAAATACTT TGCACCCGTG 
CTCAATTACC TGCGCCACGG CAAGCTTGTG CTCGATGGCG TCTCAGAGGA AGGCGTCCTG 
GAGGAGGCTG AGTTCTACAA CGTGACGCAG CTGATAGCGC TGCTGAAGGA GTGCATCCTG 
CACAGGGATC AGCGACCCCA AACGGACAAG AAGCGCGTTT ATCGTGTGCT GCAGTGCCGC 
GAGCAGGAAC TAACCCAGAT GATCTCAACG CTGTCGGATG GCTGGAGGTT CGAGCAGCTG 
ATCAGCATGC AGTACACGAA CTACGGGCCC TTCGAAAACA ATGAGTTCCT GTGCGTGGTA 
TCCAAAGAGT GTGGCACAAC GGCCGGCCGG GAGCTGGAGC TCAACGACCG GGCCAAGGTC 
CTGCAGCAGA AGGGATCGCG AATTCTTGGA ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

inc ( NM_001297858.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284787.1 Drosophila melanogaster insomniac (inc), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00488
Clone ID Related Accession (Same CDS sequence)	NM_130570.3 , NM_001297857.1 , NM_001297858.1
Accession Version	NM_001297858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	insomniac, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCACGG TGTTCATAAA CTCGCGCAAG AGCCCCAACG TGCTGAAAAA ACAGGGCACC 
GACCAGTGGG TCAAGCTGAA CGTAGGCGGT ACCTACTTCC TCACCACAAA GACGACGCTC 
TCCCGTGACC CAAATTCGTT CCTCTCCCGT CTGATTCAGG AGGACTGCGA CTTGATATCA 
GATCGGGACG AGACAGGAGC CTACCTGATC GACAGAGACC CCAAATACTT TGCACCCGTG 
CTCAATTACC TGCGCCACGG CAAGCTTGTG CTCGATGGCG TCTCAGAGGA AGGCGTCCTG 
GAGGAGGCTG AGTTCTACAA CGTGACGCAG CTGATAGCGC TGCTGAAGGA GTGCATCCTG 
CACAGGGATC AGCGACCCCA AACGGACAAG AAGCGCGTTT ATCGTGTGCT GCAGTGCCGC 
GAGCAGGAAC TAACCCAGAT GATCTCAACG CTGTCGGATG GCTGGAGGTT CGAGCAGCTG 
ATCAGCATGC AGTACACGAA CTACGGGCCC TTCGAAAACA ATGAGTTCCT GTGCGTGGTA 
TCCAAAGAGT GTGGCACAAC GGCCGGCCGG GAGCTGGAGC TCAACGACCG GGCCAAGGTC 
CTGCAGCAGA AGGGATCGCG AATTCTTGGA ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284787.1
CDS	394..1029
Translation	MSTVFINSRKSPNVLKKQGTDQWVKLNVGGTYFLTTKTTLSRDPNSFLSRLIQEDCDLISDRDETGAYLIDRDPKYFAPVLNYLRHGKLVLDGVSEEGVLEEAEFYNVTQLIALLKECILHRDQRPQTDKKRVYRVLQCREQELTQMISTLSDGWRFEQLISMQYTNYGPFENNEFLCVVSKECGTTAGRELELNDRAKVLQQKGSRILGI

Target ORF information:

RefSeq Version	NM_001297858.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster insomniac (inc), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297858.1

ORF Insert Sequence:

ATGAGCACGG TGTTCATAAA CTCGCGCAAG AGCCCCAACG TGCTGAAAAA ACAGGGCACC 
GACCAGTGGG TCAAGCTGAA CGTAGGCGGT ACCTACTTCC TCACCACAAA GACGACGCTC 
TCCCGTGACC CAAATTCGTT CCTCTCCCGT CTGATTCAGG AGGACTGCGA CTTGATATCA 
GATCGGGACG AGACAGGAGC CTACCTGATC GACAGAGACC CCAAATACTT TGCACCCGTG 
CTCAATTACC TGCGCCACGG CAAGCTTGTG CTCGATGGCG TCTCAGAGGA AGGCGTCCTG 
GAGGAGGCTG AGTTCTACAA CGTGACGCAG CTGATAGCGC TGCTGAAGGA GTGCATCCTG 
CACAGGGATC AGCGACCCCA AACGGACAAG AAGCGCGTTT ATCGTGTGCT GCAGTGCCGC 
GAGCAGGAAC TAACCCAGAT GATCTCAACG CTGTCGGATG GCTGGAGGTT CGAGCAGCTG 
ATCAGCATGC AGTACACGAA CTACGGGCCC TTCGAAAACA ATGAGTTCCT GTGCGTGGTA 
TCCAAAGAGT GTGGCACAAC GGCCGGCCGG GAGCTGGAGC TCAACGACCG GGCCAAGGTC 
CTGCAGCAGA AGGGATCGCG AATTCTTGGA ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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inc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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